2024 |
Hedley KE, Cuskelly A, Callister RJ, Horvat JC, Hodgson DM, Tadros MA, 'The medulla oblongata shows a sex-specific inflammatory response to systemic neonatal lipopolysaccharide', Journal of Neuroimmunology, 389 (2024) [C1]
Early life inflammation has been linked to long-term modulation of behavioural outcomes due to the central nervous system, but it is now becoming apparent it is also linked to dys... [more]
Early life inflammation has been linked to long-term modulation of behavioural outcomes due to the central nervous system, but it is now becoming apparent it is also linked to dysfunction of visceral physiology. The medulla oblongata contains a number of nuclei critical for homeostasis, therefore we utilised the well-established model of neonatal lipopolysaccharide (LPS) exposure to examine the immediate and long-term impacts of systemic inflammation on the medulla oblongata. Wistar rats were injected with LPS or saline on postnatal days 3 and 5, with tissues collected on postnatal days 7 or 90 in order to assess expression of inflammatory mediators and microglial morphology in autonomic regions of the medulla oblongata. We observed a distinct sex-specific response of all measured inflammatory mediators at both ages, as well as significant neonatal sex differences in inflammatory mediators within saline groups. At both ages, microglial morphology had significant changes in branch length and soma size in a sex-specific manner in response to LPS exposure. This data not only highlights the strong sex-specific response of neonates to LPS administration, but also the significant life-long impact on the medulla oblongata and the potential altered control of visceral organs.
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Nova |
2023 |
Davis OC, Dickie AC, Mustapa MB, Boyle KA, Browne TJ, Gradwell MA, et al., 'Calretinin-expressing islet cells: a source of pre- and post-synaptic inhibition of non-peptidergic nociceptor input to the mouse spinal cord.', bioRxiv, (2023)
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2023 |
Poppi LA, Bigland MJ, Cresswell ET, Tabatabaee H, Lorincz D, Drury HR, et al., 'Molecular and Functional Changes to Postsynaptic Cholinergic Signaling in the Vestibular Sensory Organs of Aging C57BL/6 Mice.', J Gerontol A Biol Sci Med Sci, 78 920-929 (2023) [C1]
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Nova |
2023 |
Davis OC, Dickie AC, Mustapa MB, Boyle KA, Browne TJ, Gradwell MA, et al., 'Calretinin-expressing islet cells are a source of pre- and post-synaptic inhibition of non-peptidergic nociceptor input to the mouse spinal cord.', Scientific reports, 13 11561 (2023) [C1]
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Nova |
2022 |
Hedley KE, Callister RJ, Callister R, Horvat JC, Tadros MA, 'Alterations in brainstem respiratory centers following peripheral inflammation: A systematic review', JOURNAL OF NEUROIMMUNOLOGY, 369 (2022) [C1]
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Nova |
2022 |
Stitt IM, Wellings TP, Drury HR, Jobling P, Callister RJ, Brichta AM, Lim R, 'Properties of Deiters? neurons and inhibitory synaptic transmission in the mouse lateral vestibular nucleus', JOURNAL OF NEUROPHYSIOLOGY, 128 131-147 (2022) [C1]
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Nova |
2022 |
Gradwell MA, Boyle KA, Browne TJ, Bell AM, Leonardo J, Reyes FSP, et al., 'Diversity of inhibitory and excitatory parvalbumin interneuron circuits in the dorsal horn', PAIN, 163 E432-E452 (2022) [C1]
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Nova |
2022 |
Iredale JA, Stoddard JG, Drury HR, Browne TJ, Elton A, Madden JF, et al., 'Recording Network Activity in Spinal Nociceptive Circuits using Microelectrode Arrays', JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, (2022) [C1]
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Nova |
2022 |
Gradwell MA, Smith KM, Dayas CV, Smith DW, Hughes DI, Callister RJ, Graham BA, 'Altered Intrinsic Properties and Inhibitory Connectivity in Aged Parvalbumin-Expressing Dorsal Horn Neurons', FRONTIERS IN NEURAL CIRCUITS, 16 (2022) [C1]
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Nova |
2021 |
Quinn RK, Drury HR, Lim R, Callister RJ, Tadros MA, 'Differentiation of Sensory Neuron Lineage During the Late First and Early Second Trimesters of Human Foetal Development', NEUROSCIENCE, 467 28-38 (2021) [C1]
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Nova |
2021 |
Quinn RK, Drury HR, Cresswell ET, Tadros MA, Nayagam BA, Callister RJ, et al., 'Expression and Physiology of Voltage-Gated Sodium Channels in Developing Human Inner Ear', FRONTIERS IN NEUROSCIENCE, 15 (2021) [C1]
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Nova |
2021 |
Browne TJ, Smith KM, Gradwell MA, Iredale JA, Dayas CV, Callister RJ, et al., 'Spinoparabrachial projection neurons form distinct classes in the mouse dorsal horn', PAIN, 162 1977-1994 (2021) [C1]
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Nova |
2020 |
Madden JF, Davis OC, Boyle KA, Iredale JA, Browne TJ, Callister RJ, et al., 'Functional and Molecular Analysis of Proprioceptive Sensory Neuron Excitability in Mice', Frontiers in Molecular Neuroscience, 13 1-13 (2020) [C1]
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Nova |
2020 |
Callister RJ, Brichta AM, Schaefer AT, Graham BA, Stuart DG, 'Pioneers in CNS inhibition: 2. Charles Sherrington and John Eccles on inhibition in spinal and supraspinal structures', BRAIN RESEARCH, 1734 (2020) [C1]
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Nova |
2020 |
Gradwell MA, Callister RJ, Graham BA, 'Reviewing the case for compromised spinal inhibition in neuropathic pain', Journal of Neural Transmission, 127 481-503 (2020) [C1]
A striking and debilitating property of the nervous system is that damage to this tissue can cause chronic intractable pain, which persists long after resolution of the initial in... [more]
A striking and debilitating property of the nervous system is that damage to this tissue can cause chronic intractable pain, which persists long after resolution of the initial insult. This neuropathic form of pain can arise from trauma to peripheral nerves, the spinal cord, or brain. It can also result from neuropathies associated with disease states such as diabetes, human immunodeficiency virus/AIDS, herpes, multiple sclerosis, cancer, and chemotherapy. Regardless of the origin, treatments for neuropathic pain remain inadequate. This continues to drive research into the underlying mechanisms. While the literature shows that dysfunction in numerous loci throughout the CNS can contribute to chronic pain, the spinal cord and in particular inhibitory signalling in this region have remained major research areas. This review focuses on local spinal inhibition provided by dorsal horn interneurons, and how such inhibition is disrupted during the development and maintenance of neuropathic pain.
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Nova |
2020 |
Browne TJ, Hughes DI, Dayas CV, Callister RJ, Graham BA, 'Projection Neuron Axon Collaterals in the Dorsal Horn: Placing a New Player in Spinal Cord Pain Processing', FRONTIERS IN PHYSIOLOGY, 11 (2020) [C1]
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Nova |
2020 |
Mayhew JA, Cummins MJ, Cresswell ET, Callister RJ, Smith DW, Graham BA, 'Age-related gene expression changes in lumbar spinal cord: Implications for neuropathic pain', Molecular Pain, 16 (2020) [C1]
Clinically, pain has an uneven incidence throughout lifespan and impacts more on the elderly. In contrast, preclinical models of pathological pain have typically used juvenile or ... [more]
Clinically, pain has an uneven incidence throughout lifespan and impacts more on the elderly. In contrast, preclinical models of pathological pain have typically used juvenile or young adult animals to highlight the involvement of glial populations, proinflammatory cytokines, and chemokines in the onset and maintenance of pathological signalling in the spinal dorsal horn. The potential impact of this mismatch is also complicated by the growing appreciation that the aged central nervous system exists in a state of chronic inflammation because of enhanced proinflammatory cytokine/chemokine signalling and glial activation. To address this issue, we investigated the impact of aging on the expression of genes that have been associated with neuropathic pain, glial signalling, neurotransmission and neuroinflammation. We used qRT-PCR to quantify gene expression and focussed on the dorsal horn of the spinal cord as this is an important perturbation site in neuropathic pain. To control for global vs region-specific age-related changes in gene expression, the ventral half of the spinal cord was examined. Our results show that expression of proinflammatory chemokines, pattern recognition receptors, and neurotransmitter system components was significantly altered in aged (24¿32 months) versus young mice (2¿4 months). Notably, the magnitude and direction of these changes were spinal-cord region dependent. For example, expression of the chemokine, Cxcl13, increased 119-fold in dorsal spinal cord, but only 2-fold in the ventral spinal cord of old versus young mice. Therefore, we propose the dorsal spinal cord of old animals is subject to region-specific alterations that prime circuits for the development of pathological pain, potentially in the absence of the peripheral triggers normally associated with these conditions.
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Nova |
2020 |
Browne TJ, Gradwell MA, Iredale JA, Maden JF, Callister RJ, Hughes DI, et al., 'Transgenic Cross-Referencing of Inhibitory and Excitatory Interneuron Populations to Dissect Neuronal Heterogeneity in the Dorsal Horn', Frontiers in Molecular Neuroscience, 13 1-20 (2020) [C1]
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Nova |
2019 |
Mayhew JA, Callister RJ, Walker FR, Smith DW, Graham BA, 'Aging alters signaling properties in the mouse spinal dorsal horn', MOLECULAR PAIN, 15 (2019) [C1]
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Nova |
2019 |
Boyle KA, Gradwell MA, Yasaka T, Dickie AC, Polgár E, Ganley RP, et al., 'Defining a Spinal Microcircuit that Gates Myelinated Afferent Input: Implications for Tactile Allodynia', Cell Reports, 28 526-540.e6 (2019) [C1]
Chronic pain presents a major unmet clinical problem. The development of more effective treatments is hindered by our limited understanding of the neuronal circuits underlying sen... [more]
Chronic pain presents a major unmet clinical problem. The development of more effective treatments is hindered by our limited understanding of the neuronal circuits underlying sensory perception. Here, we show that parvalbumin (PV)-expressing dorsal horn interneurons modulate the passage of sensory information conveyed by low-threshold mechanoreceptors (LTMRs) directly via presynaptic inhibition and also gate the polysynaptic relay of LTMR input to pain circuits by inhibiting lamina II excitatory interneurons whose axons project into lamina I. We show changes in the functional properties of these PV interneurons following peripheral nerve injury and that silencing these cells unmasks a circuit that allows innocuous touch inputs to activate pain circuits by increasing network activity in laminae I¿IV. Such changes are likely to result in the development of tactile allodynia and could be targeted for more effective treatment of mechanical pain. In this study, Boyle et al. identify parvalbumin-expressing spinal interneurons as a principal source of axoaxonic synapses onto cutaneous myelinated afferents and inhibitory inputs onto lamina II vertical cells. Following peripheral nerve injury, disinhibition of these targets facilitates the aberrant recruitment of pain circuits, leading to tactile allodynia.
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Nova |
2019 |
de Oliveira RB, Petiz LL, Lim R, Lipski J, Gravina FS, Brichta AM, et al., 'Crosstalk between mitochondria, calcium channels and actin cytoskeleton modulates noradrenergic activity of locus coeruleus neurons.', Journal of neurochemistry, 149 471-487 (2019) [C1]
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Nova |
2019 |
Smith KM, Browne TJ, Davis OC, Coyle A, Boyle KA, Watanabe M, et al., 'Calretinin positive neurons form an excitatory amplifier network in the spinal cord dorsal horn', ELIFE, 8 (2019) [C1]
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Nova |
2018 |
Rank MM, Galea MP, Callister R, Callister RJ, 'Is more always better? How different doses of exercise after incomplete spinal cord injury affects the membrane properties of deep dorsal horn interneurons', Experimental Neurology, 300 201-211 (2018) [C1]
Interneurons in the deep dorsal horn (DDH) of the spinal cord process somatosensory input, and form an important link between upper and lower motoneurons to subsequently shape mot... [more]
Interneurons in the deep dorsal horn (DDH) of the spinal cord process somatosensory input, and form an important link between upper and lower motoneurons to subsequently shape motor output. Exercise training after SCI is known to improve functional motor recovery, but little is known about the mechanisms within spinal cord neurons that underlie these improvements. Here we investigate how the properties of DDH interneurons are affected by spinal cord injury (SCI) alone, and SCI in combination with different ¿doses¿ of treadmill exercise training (3, 6, and 9 wks). In an adult mouse hemisection model of SCI we used whole-cell patch-clamp electrophysiology to record intrinsic, AP firing and gain modulation properties from DDH interneurons in a horizontal spinal cord slice preparation. We find that neurons within two segments of the injury, both ipsi- and contralateral to the hemisection, are similarly affected by SCI and SCI plus exercise. The passive intrinsic membrane properties input resistance (Rin) and rheobase are sensitive to the effects of recovery time and exercise training after SCI thus altering DDH interneuron excitability. Conversely, select active membrane properties are largely unaffected by either SCI or exercise training. SCI itself causes a mismatch in the expression of voltage-gated subthreshold currents and AP discharge firing type. Over time after SCI, and especially with exercise training (9 wks), this mismatched expression is exacerbated. Lastly, amplification properties (i.e. gain of frequency-current relationship) of DDH interneurons are altered by SCI alone and recover spontaneously with no clear effect of exercise training. These results suggest a larger ¿dose¿ of exercise training (9 wks) has a strong and selective effect on specific membrane properties, and on the output of interneurons in the vicinity of a SCI. These electrophysiological data provide new insights into the plasticity of DDH interneurons and the mechanisms by which exercise therapy after SCI can improve recovery.
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Nova |
2018 |
Mateer SW, Mathe A, Bruce J, Liu G, Maltby S, Fricker M, et al., 'IL-6 Drives Neutrophil-Mediated Pulmonary Inflammation Associated with Bacteremia in Murine Models of Colitis', American Journal of Pathology, 188 1625-1639 (2018) [C1]
Inflammatory bowel disease (IBD) is associated with several immune-mediated extraintestinal manifestations. More than half of all IBD patients have some form of respiratory pathol... [more]
Inflammatory bowel disease (IBD) is associated with several immune-mediated extraintestinal manifestations. More than half of all IBD patients have some form of respiratory pathology, most commonly neutrophil-mediated diseases, such as bronchiectasis and chronic bronchitis. Using murine models of colitis, we aimed to identify the immune mechanisms driving pulmonary manifestations of IBD. We found increased neutrophil numbers in lung tissue associated with the pulmonary vasculature in both trinitrobenzenesulfonic acid¿ and dextran sulfate sodium¿induced models of colitis. Analysis of systemic inflammation identified that neutrophilia was associated with bacteremia and pyrexia in animal models of colitis. We further identified IL-6 as a systemic mediator of neutrophil recruitment from the bone marrow of dextran sulfate sodium animals. Functional inhibition of IL-6 led to reduced systemic and pulmonary neutrophilia, but it did not attenuate established colitis pathology. These data suggest that systemic bacteremia and pyrexia drive IL-6 secretion, which is a critical driver for pulmonary manifestation of IBD. Targeting IL-6 may reduce neutrophil-associated extraintestinal manifestations in IBD patients.
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Nova |
2018 |
Poppi LA, Tabatabaee H, Drury HR, Jobling P, Callister RJ, Migliaccio AA, et al., 'ACh-induced hyperpolarization and decreased resistance in mammalian type II vestibular hair cells', Journal of Neurophysiology, 119 312-325 (2018) [C1]
In the mammalian vestibular periphery, electrical activation of the efferent vestibular system (EVS) has two effects on afferent activity: 1) it increases background afferent disc... [more]
In the mammalian vestibular periphery, electrical activation of the efferent vestibular system (EVS) has two effects on afferent activity: 1) it increases background afferent discharge and 2) decreases afferent sensitivity to rotational stimuli. Although the cellular mechanisms underlying these two contrasting afferent responses remain obscure, we postulated that the reduction in afferent sensitivity was attributed, in part, to the activation of a9- containing nicotinic acetylcholine (ACh) receptors (a9*nAChRs) and small-conductance potassium channels (SK) in vestibular type II hair cells, as demonstrated in the peripheral vestibular system of other vertebrates. To test this hypothesis, we examined the effects of the predominant EVS neurotransmitter ACh on vestibular type II hair cells from wild-type (wt) and a9-subunit nAChR knockout (a9 -/- ) mice. Immunostaining for choline acetyltransferase revealed there were no obvious gross morphological differences in the peripheral EVS innervation among any of these strains. ACh application onto wt type II hair cells, at resting potentials, produced a fast inward current followed by a slower outward current, resulting in membrane hyperpolarization and decreased membrane resistance. Hyperpolarization and decreased resistance were due to gating of SK channels. Consistent with activation of a9*nAChRs and SK channels, these ACh-sensitive currents were antagonized by the a9*nAChR blocker strychnine and SK blockers apamin and tamapin. Type II hair cells from a9 -/- mice, however, failed to respond to ACh at all. These results confirm the critical importance of a9nAChRs in efferent modulation of mammalian type II vestibular hair cells. Application of exogenous ACh reduces electrical impedance, thereby decreasing type II hair cell sensitivity. NEW & NOTEWORTHY Expression of a9 nicotinic subunit was crucial for fast cholinergic modulation of mammalian vestibular type II hair cells. These findings show a multifaceted efferent mechanism for altering hair cell membrane potential and decreasing membrane resistance that should reduce sensitivity to hair bundle displacements.
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Nova |
2018 |
Tadros MA, Graham BA, Callister RJ, 'Moving functional classification of dorsal horn neurons from art to science', JOURNAL OF PHYSIOLOGY-LONDON, 596 1543-1544 (2018)
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2018 |
Poppi LA, Tabatabaee H, Jobling P, Callister RJ, Migliaccio AA, Jordan PM, et al., 'ACh-induced hyperpolarization and decreased resistance in mammalian type II vestibular hair cells (vol 119, pg 312, 2018)', JOURNAL OF NEUROPHYSIOLOGY, 120 385-385 (2018)
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2018 |
Tadros MA, Zouikr I, Hodgson DM, Callister RJ, 'Excitability of rat superficial dorsal horn neurons following a neonatal immune challenge', Frontiers in Neurology, 9 (2018) [C1]
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Nova |
2017 |
Gradwell MA, Boyle KA, Callister RJ, Hughes DI, Graham BA, 'Heteromeric a/ß glycine receptors regulate excitability in parvalbumin-expressing dorsal horn neurons through phasic and tonic glycinergic inhibition', Journal of Physiology, 595 7185-7202 (2017) [C1]
Key points: Spinal parvalbumin-expressing interneurons have been identified as a critical source of inhibition to regulate sensory thresholds by gating mechanical inputs in the do... [more]
Key points: Spinal parvalbumin-expressing interneurons have been identified as a critical source of inhibition to regulate sensory thresholds by gating mechanical inputs in the dorsal horn. This study assessed the inhibitory regulation of the parvalbumin-expressing interneurons, showing that synaptic and tonic glycinergic currents dominate, blocking neuronal or glial glycine transporters enhances tonic glycinergic currents, and these manipulations reduce excitability. Synaptically released glycine also enhanced tonic glycinergic currents and resulted in decreased parvalbumin-expressing interneuron excitability. Analysis of the glycine receptor properties mediating inhibition of parvalbumin neurons, as well as single channel recordings, indicates that heteromeric a/ß subunit-containing receptors underlie both synaptic and tonic glycinergic currents. Our findings indicate that glycinergic inhibition provides critical control of excitability in parvalbumin-expressing interneurons in the dorsal horn and represents a pharmacological target to manipulate spinal sensory processing. Abstract: The dorsal horn (DH) of the spinal cord is an important site for modality-specific processing of sensory information and is essential for contextually relevant sensory experience. Parvalbumin-expressing inhibitory interneurons (PV+ INs) have functional properties and connectivity that enables them to segregate tactile and nociceptive information. Here we examine inhibitory drive to PV+ INs using targeted patch-clamp recording in spinal cord slices from adult transgenic mice that express enhanced green fluorescent protein in PV+ INs. Analysis of inhibitory synaptic currents showed glycinergic transmission is the dominant form of phasic inhibition to PV+ INs. In addition, PV+ INs expressed robust glycine-mediated tonic currents; however, we found no evidence for tonic GABAergic currents. Manipulation of extracellular glycine by blocking either, or both, the glial and neuronal glycine transporters markedly decreased PV+ IN excitability, as assessed by action potential discharge. This decreased excitability was replicated when tonic glycinergic currents were increased by electrically activating glycinergic synapses. Finally, we show that both phasic and tonic forms of glycinergic inhibition are mediated by heteromeric a/ß glycine receptors. This differs from GABAA receptors in the dorsal horn, where different receptor stoichiometries underlie phasic and tonic inhibition. Together these data suggest both phasic and tonic glycinergic inhibition regulate the output of PV+ INs and contribute to the processing and segregation of tactile and nociceptive information. The shared stoichiometry for phasic and tonic glycine receptors suggests pharmacology is unlikely to be able to selectively target each form of inhibition in PV+ INs.
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Nova |
2017 |
Farrell KE, Keely S, Walker MM, Brichta AM, Graham BA, Callister RJ, 'Altered intrinsic and synaptic properties of lumbosacral dorsal horn neurons in a mouse model of colitis', Neuroscience, 362 152-167 (2017) [C1]
Visceral pain in inflammatory and functional gastrointestinal conditions is a major clinical problem. The exact mechanisms underlying the development of pain, during and after vis... [more]
Visceral pain in inflammatory and functional gastrointestinal conditions is a major clinical problem. The exact mechanisms underlying the development of pain, during and after visceral inflammation are unknown. However, clinical and pre-clinical evidence suggests plasticity within the spinal cord dorsal horn is a contributing factor. Here we use an in vivo preparation and patch-clamp electrophysiology to test whether the synaptic and intrinsic properties of superficial dorsal horn (SDH) neurons are altered 5 days after the induction of mild colitis in adult male mice (i.e. during acute inflammation of the colon). Whole-cell recordings were made from lumbosacral (L6-S1) superficial dorsal horn neurons (SDH), in animals under isoflurane anesthesia. Noxious colorectal distension (CRD) was used to identify SDH neurons with colonic inputs, while stimulation of the hind paw and tail was employed to assess convergent cutaneous input. Following inflammation, a significantly increased proportion of SDH neurons received both colonic and cutaneous inputs, compared to neurons in naïve animals. In addition, the nature and magnitude of responses to CRD and cutaneous stimulation differed in inflamed animals, as was spontaneous excitatory synaptic drive. Conversely, several measures of intrinsic excitability were altered in a manner that would decrease SDH network excitability following colitis. We propose that during inflammation, sensitization of colonic afferents results in increased signaling to the SDH. This is accompanied by plasticity in SDH neurons whereby their intrinsic properties are changed to compensate for altered afferent activity.
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Nova |
2017 |
Battistuzzo CR, Rank MM, Flynn JR, Morgan DL, Callister R, Callister RJ, Galea MP, 'Effects Of treadmill training on hindlimb muscles of spinal cord injured mice', Muscle and Nerve, 55 232-242 (2017) [C1]
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Nova |
2017 |
Flynn JR, Conn VL, Boyle KA, Hughes DI, Watanabe M, Velasquez T, et al., 'Anatomical and Molecular Properties of Long Descending Propriospinal Neurons in Mice', FRONTIERS IN NEUROANATOMY, 11 (2017) [C1]
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Nova |
2017 |
Marks E, Naudin C, Nolan G, Goggins BJ, Burns G, Mateer SW, et al., 'Regulation of IL-12p40 by HIF controls Th1/Th17 responses to prevent mucosal inflammation', MUCOSAL IMMUNOLOGY, 10 1224-1236 (2017) [C1]
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Nova |
2016 |
Tadros MA, Fuglevand AJ, Brichta AM, Callister RJ, 'Intrinsic excitability differs between murine hypoglossal and spinal motoneurons.', Journal of neurophysiology, 115 2672-2680 (2016) [C1]
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Nova |
2016 |
Farrell KE, Rank MM, Keely S, Brichta AM, Graham BA, Callister RJ, 'In vivo characterization of colorectal and cutaneous inputs to lumbosacral dorsal horn neurons in the mouse spinal cord', Neuroscience, 316 13-25 (2016) [C1]
Chronic abdominal pain is a common symptom of inflammatory bowel disease and often persists in the absence of gut inflammation. Although the mechanisms responsible for ongoing pai... [more]
Chronic abdominal pain is a common symptom of inflammatory bowel disease and often persists in the absence of gut inflammation. Although the mechanisms responsible for ongoing pain are unknown, clinical and preclinical evidence suggests lumbosacral spinal cord dorsal horn neurons contribute to these symptoms. At present, we know little about the intrinsic and synaptic properties of this population of neurons in either normal or inflammed conditions. Therefore, we developed an in vivo preparation to make patch-clamp recordings from superficial dorsal horn (SDH) neurons receiving colonic inputs in naïve male mice. Recordings were made in the lumbosacral spinal cord (L6-S1) under isoflurane anesthesia. Noxious colorectal distension (CRD) was used to determine whether SDH neurons received inputs from mechanical stimulation/distension of the colon. Responses to hind paw/tail cutaneous stimulation and intrinsic and synaptic properties were also assessed, as well as action potential discharge properties. Approximately 11% of lumbosacral SDH neurons in the cohort of neurons sampled responded to CRD and a majority of these responses were subthreshold. Most CRD-responsive neurons (80%) also responded to cutaneous stimuli, compared with <50% of CRD-non-responsive neurons. Furthermore, CRD-responsive neurons had more hyperpolarized resting membrane potentials, larger rheobase currents, and reduced levels of excitatory drive, compared to CRD-non-responsive neurons. Our results demonstrate that CRD-responsive neurons can be distinguished from CRD-non-responsive neurons by several differences in their membrane properties and excitatory synaptic inputs. We also demonstrate that SDH neurons with colonic inputs show predominately subthreshold responses to CRD and exhibit a high degree of viscerosomatic convergence.
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Nova |
2016 |
Battistuzzo CR, Rank MM, Flynn JR, Morgan DL, Callister R, Callister RJ, Galea MP, 'Gait recovery following spinal cord injury in mice: Limited effect of treadmill training', Journal of Spinal Cord Medicine, 39 335-343 (2016) [C1]
Several studies in rodents with complete spinal cord transections have demonstrated that treadmill training improves stepping movements. However, results from studies in incomplet... [more]
Several studies in rodents with complete spinal cord transections have demonstrated that treadmill training improves stepping movements. However, results from studies in incomplete spinal cord injured animals have been conflicting and questions regarding the training dosage after injury remain unresolved. Objectives: To assess the effects of treadmill-training regimen (20 minutes daily, 5 days a week) for 3, 6 or 9 weeks on the recovery of locomotion in hemisected SCI mice. Methods: A randomized and blinded controlled experimental trial used a mouse model of incomplete spinal cord injury (SCI). After a left hemisection at T10, adult male mice were randomized to trained or untrained groups. The trained group commenced treadmill training one week after surgery and continued for 3, 6 or 9 weeks. Quantitative kinematic gait analysis was used to assess the spatiotemporal characteristics of the left hindlimb prior to injury and at 1, 4, 7 and 10 weeks post-injury. Results: One week after injury there was no movement of the left hindlimb and some animals dragged their foot. Treadmill training led to significant improvements in step duration, but had limited effect on the hindlimb movement pattern. Locomotor improvements in trained animals were most evident at the hip and knee joints whereas recovery of ankle movement was limited, even after 9 weeks of treadmill training. Conclusion: These results demonstrate that treadmill training may lead to only modest improvement in recovery of hindlimb movement after incomplete spinal cord injury in mice.
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Nova |
2016 |
Smith KM, Boyle KA, Mustapa M, Jobling P, Callister RJ, Hughes DI, Graham BA, 'Distinct forms of synaptic inhibition and neuromodulation regulate calretinin-positive neuron excitability in the spinal cord dorsal horn', Neuroscience, 326 10-21 (2016) [C1]
The dorsal horn (DH) of the spinal cord contains a heterogenous population of neurons that process incoming sensory signals before information ascends to the brain. We have recent... [more]
The dorsal horn (DH) of the spinal cord contains a heterogenous population of neurons that process incoming sensory signals before information ascends to the brain. We have recently characterized calretinin-expressing (CR+) neurons in the DH and shown that they can be divided into excitatory and inhibitory subpopulations. The excitatory population receives high-frequency excitatory synaptic input and expresses delayed firing action potential discharge, whereas the inhibitory population receives weak excitatory drive and exhibits tonic or initial bursting discharge. Here, we characterize inhibitory synaptic input and neuromodulation in the two CR+ populations, in order to determine how each is regulated. We show that excitatory CR+ neurons receive mixed inhibition from GABAergic and glycinergic sources, whereas inhibitory CR+ neurons receive inhibition, which is dominated by glycine. Noradrenaline and serotonin produced robust outward currents in excitatory CR+ neurons, predicting an inhibitory action on these neurons, but neither neuromodulator produced a response in CR+ inhibitory neurons. In contrast, enkephalin (along with selective mu and delta opioid receptor agonists) produced outward currents in inhibitory CR+ neurons, consistent with an inhibitory action but did not affect the excitatory CR+ population. Our findings show that the pharmacology of inhibitory inputs and neuromodulator actions on CR+ cells, along with their excitatory inputs can define these two subpopulations further, and this could be exploited to modulate discrete aspects of sensory processing selectively in the DH.
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Nova |
2015 |
Rank MM, Flynn JR, Battistuzzo CR, Galea MP, Callister R, Callister RJ, 'Functional changes in deep dorsal horn interneurons following spinal cord injury are enhanced with different durations of exercise training', JOURNAL OF PHYSIOLOGY-LONDON, 593 331-345 (2015) [C1]
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Nova |
2015 |
Rank MM, Flynn JR, Galea MP, Callister R, Callister RJ, 'Electrophysiological characterization of spontaneous recovery in deep dorsal horn interneurons after incomplete spinal cord injury', Experimental Neurology, 271 468-478 (2015) [C1]
In the weeks and months following an incomplete spinal cord injury (SCI) significant spontaneous recovery of function occurs in the absence of any applied therapeutic intervention... [more]
In the weeks and months following an incomplete spinal cord injury (SCI) significant spontaneous recovery of function occurs in the absence of any applied therapeutic intervention. The anatomical correlates of this spontaneous plasticity are well characterized, however, the functional changes that occur in spinal cord interneurons after injury are poorly understood. Here we use a T10 hemisection model of SCI in adult mice (9-10 wks old) combined with whole-cell patch clamp electrophysiology and a horizontal spinal cord slice preparation to examine changes in intrinsic membrane and synaptic properties of deep dorsal horn (DDH) interneurons. We made these measurements during short-term (4 wks) and long-term (10 wks) spontaneous recovery after SCI. Several important intrinsic membrane properties are altered in the short-term, but recover to values resembling those of uninjured controls in the longer term. AP discharge patterns are reorganized at both short-term and long-term recovery time points. This is matched by reorganization in the expression of voltage-activated potassium and calcium subthreshold-currents that shape AP discharge. Excitatory synaptic inputs onto DDH interneurons are significantly restructured in long-term SCI mice. Plots of sEPSC peak amplitude vs. rise times suggest considerable dendritic expansion or synaptic reorganization occurs especially during long-term recovery from SCI. Connectivity between descending dorsal column pathways and DDH interneurons is reduced in the short-term, but amplified in long-term recovery. Our results suggest considerable plasticity in both intrinsic and synaptic mechanisms occurs spontaneously in DDH interneurons following SCI and takes a minimum of 10 wks after the initial injury to stabilize.
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Nova |
2015 |
Tadros MA, Lim R, Hughes DI, Brichta AM, Callister RJ, 'Electrical maturation of spinal neurons in the human fetus: Comparison of ventral and dorsal horn', Journal of Neurophysiology, 114 2661-2671 (2015) [C1]
The spinal cord is critical for modifying and relaying sensory information to, and motor commands from, higher centers in the central nervous system to initiate and maintain conte... [more]
The spinal cord is critical for modifying and relaying sensory information to, and motor commands from, higher centers in the central nervous system to initiate and maintain contextually relevant locomotor responses. Our understanding of how spinal sensorimotor circuits are established during in utero development is based largely on studies in rodents. In contrast, there is little functional data on the development of sensory and motor systems in humans. Here, we use patch-clamp electrophysiology to examine the development of neuronal excitability in human fetal spinal cords (10¿18 wk gestation; WG). Transverse spinal cord slices (300 µm thick) were prepared, and recordings were made, from visualized neurons in either the ventral (VH) or dorsal horn (DH) at 32°C. Action potentials (APs) could be elicited in VH neurons throughout the period examined, but only after 16 WG in DH neurons. At this age, VH neurons discharged multiple APs, whereas most DH neurons discharged single APs. In addition, at 16¿18 WG, VH neurons also displayed larger AP and after-hyperpolarization amplitudes than DH neurons. Between 10 and 18 WG, the intrinsic properties of VH neurons changed markedly, with input resistance decreasing and AP and after-hyperpolarization amplitudes increasing. These findings are consistent with the hypothesis that VH motor circuitry matures more rapidly than the DH circuits that are involved in processing tactile and nociceptive information.
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Nova |
2015 |
Tadros MA, Farrell KE, Graham BA, Brichta AM, Callister RJ, 'Properties of sodium currents in neonatal and young adult mouse superficial dorsal horn neurons', Molecular Pain, 11 (2015) [C1]
Background: Superficial dorsal horn (SDH) neurons process nociceptive information and their excitability is partly determined by the properties of voltage-gated sodium channels. R... [more]
Background: Superficial dorsal horn (SDH) neurons process nociceptive information and their excitability is partly determined by the properties of voltage-gated sodium channels. Recently, we showed the excitability and action potential properties of mouse SDH neurons change markedly during early postnatal development. Here we compare sodium currents generated in neonate (P0-5) and young adult (=P21) SDH neurons. Results: Whole cell recordings were obtained from lumbar SDH neurons in transverse spinal cord slices (CsF internal, 32°C). Fast activating and inactivating TTX-sensitive inward currents were evoked by depolarization from a holding potential of 100mV. Poorly clamped currents, based on a deflection in the IV relationship at potentials between 60 and 50mV, were not accepted for analysis. Current density and decay time increased significantly between the first and third weeks of postnatal development, whereas time to peak was similar at both ages. This was accompanied by more subtle changes in activation range and steady state inactivation. Recovery from inactivation was slower and TTX-sensitivity was reduced in young adult neurons. Conclusions: Our study suggests sodium channel expression changes markedly during early postnatal development in mouse SDH neurons. The methods employed in this study can now be applied to future investigations of spinal cord sodium channel plasticity in murine pain models.
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Nova |
2015 |
Tadros MA, Farrell KE, Graham BA, Brichta AM, Callister RJ, 'Properties of sodium currents in neonatal and young adult mouse superficial dorsal horn neurons.', Molecular pain, 11 17 (2015)
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2015 |
Callister RJ, Graham BA, 'Spicing up the gabapentionoids: Facilitating gabapentin entry in spinal pain circuits', Neuroscience Letters, 584 395-396 (2015) [C3]
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2015 |
Smith KM, Boyle KA, Madden JF, Dickinson SA, Jobling P, Callister RJ, et al., 'Functional heterogeneity of calretinin-expressing neurons in the mouse superficial dorsal horn: Implications for spinal pain processing', Journal of Physiology, 593 4319-4339 (2015) [C1]
Neurons in the superficial dorsal horn (SDH) of the spinal cord play an important role in nociceptive, thermal, itch and light touch sensations. Excitatory interneurons comprise ~... [more]
Neurons in the superficial dorsal horn (SDH) of the spinal cord play an important role in nociceptive, thermal, itch and light touch sensations. Excitatory interneurons comprise ~65% of all SDH neurons but surprisingly few studies have investigated their role in spinal sensory processing. Here we use a transgenic mouse to study putative excitatory SDH neurons that express the calcium binding protein calretinin (CR). Our immunocytochemical, morphological and electrophysiological analysis identified two distinct populations of CR-expressing neurons, which we termed 'Typical' and 'Atypical'. Typical CR-expressing neurons comprised ~85% of the population and exhibited characteristic excitatory interneuron properties including delayed firing discharge, large rapid A-type potassium currents, and central, radial or vertical cell morphologies. Atypical neurons exhibited properties consistent with inhibitory interneurons, including tonic firing or initial bursting discharge, Ih currents, and islet cell morphology. Although both Typical and Atypical CR-expressing neurons responded to noxious peripheral stimulation, the excitatory drive onto Typical CR-expressing neurons was much stronger. Furthermore, Atypical CR-expressing cells comprise at least two functionally distinct subpopulations based on their responsiveness to noxious peripheral stimulation and neurochemical profile. Together our data suggest CR expression is not restricted to excitatory neurons in the SDH. Under normal conditions, the contribution of 'Typical' excitatory CR-expressing neurons to overall SDH excitability may be limited by the presence of A-type potassium currents, which limit the effectiveness of their strong excitatory input. Their contribution may, however, be increased in pathological situations where A-type potassium currents are decreased. By contrast, 'Atypical' inhibitory neurons with their excitable phenotype but weak excitatory input may be more easily recruited during increased peripheral stimulation.
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Nova |
2014 |
Tadros MA, Farrell KE, Schofield PR, Brichta AM, Graham BA, Fuglevand AJ, Callister RJ, 'Intrinsic and synaptic homeostatic plasticity in motoneurons from mice with glycine receptor mutations', Journal of Neurophysiology, 111 1487-1498 (2014) [C1]
Inhibitory synaptic inputs to hypoglossal motoneurons (HMs) are important for modulating excitability in brainstem circuits. Here we ask whether reduced inhibition, as occurs in t... [more]
Inhibitory synaptic inputs to hypoglossal motoneurons (HMs) are important for modulating excitability in brainstem circuits. Here we ask whether reduced inhibition, as occurs in three murine mutants with distinct naturally occurring mutations in the glycine receptor (GlyR), leads to intrinsic and/or synaptic homeostatic plasticity. Whole cell recordings were obtained from HMs in transverse brainstem slices from wild-type (wt), spasmodic (spd), spastic (spa), and oscillator (ot) mice (C57Bl/6, approximately postnatal day 21). Passive and action potential (AP) properties in spd and ot HMs were similar to wt. In contrast, spa HMs had lower input resistances, more depolarized resting membrane potentials, higher rheobase currents, smaller AP amplitudes, and slower afterhyperpolarization current decay times. The excitability of HMs, assessed by "gain" in injected current/firing-frequency plots, was similar in all strains whereas the incidence of rebound spiking was increased in spd. The difference between recruitment and derecruitment current (i.e., ¿I) for AP discharge during ramp current injection was more negative in spa and ot. GABAA miniature inhibitory postsynaptic current (mIPSC) amplitude was increased in spa and ot but not spd, suggesting diminished glycinergic drive leads to compensatory adjustments in the other major fast inhibitory synaptic transmitter system in these mutants. Overall, our data suggest long-term reduction in glycinergic drive to HMs results in changes in intrinsic and synaptic properties that are consistent with homeostatic plasticity in spa and ot but not in spd. We propose such plasticity is an attempt to stabilize HM output, which succeeds in spa but fails in ot. © 2014 the American Physiological Society.
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Nova |
2014 |
Zouikr I, Tadros MA, Barouei J, Beagley KW, Clifton VL, Callister RJ, Hodgson DM, 'Altered nociceptive, endocrine, and dorsal horn neuron responses in rats following a neonatal immune challenge', PSYCHONEUROENDOCRINOLOGY, 41 1-12 (2014) [C1]
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Nova |
2014 |
Farrell KE, Keely S, Graham BA, Callister R, Callister RJ, 'A Systematic Review of the Evidence for Central Nervous System Plasticity in Animal Models of Inflammatory-mediated Gastrointestinal Pain', INFLAMMATORY BOWEL DISEASES, 20 176-195 (2014) [C1]
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Nova |
2014 |
Lim R, Drury HR, Tadros MA, Callister RJ, Brichta AM, Camp AJ, 'Preliminary Characterization of Voltage-Activated Whole-Cell Currents in Developing Human Vestibular Hair Cells and Calyx Afferent Terminals', Journal of the Association for Research in Otolaryngology, (2014) [C1]
We present preliminary functional data from human vestibular hair cells and primary afferent calyx terminals during fetal development. Whole-cell recordings were obtained from hai... [more]
We present preliminary functional data from human vestibular hair cells and primary afferent calyx terminals during fetal development. Whole-cell recordings were obtained from hair cells or calyx terminals in semi-intact cristae prepared from human fetuses aged between 11 and 18 weeks gestation (WG). During early fetal development (11-14 WG), hair cells expressed whole-cell conductances that were qualitatively similar but quantitatively smaller than those observed previously in mature rodent type II hair cells. As development progressed (15-18 WG), peak outward conductances increased in putative type II hair cells but did not reach amplitudes observed in adult human hair cells. Type I hair cells express a specific low-voltage activating conductance, G. A similar current was first observed at 15 WG but remained relatively small, even at 18 WG. The presence of a "collapsing" tail current indicates a maturing type I hair cell phenotype and suggests the presence of a surrounding calyx afferent terminal. We were also able to record from calyx afferent terminals in 15-18 WG cristae. In voltage clamp, these terminals exhibited fast inactivating inward as well as slower outward conductances, and in current clamp, discharged a single action potential during depolarizing steps. Together, these data suggest the major functional characteristics of type I and type II hair cells and calyx terminals are present by 18 WG. Our study also describes a new preparation for the functional investigation of key events that occur during maturation of human vestibular organs. © 2014 The Author(s).
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Nova |
2014 |
Farrell KE, Callister RJ, Keely S, 'Understanding and targeting centrally mediated visceral pain in inflammatory bowel disease', Frontiers in Pharmacology, 5 1-4 (2014) [C3]
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Nova |
2014 |
Harris BM, Hughes DI, Bolton PS, Tadros MA, Callister RJ, Graham BA, 'Contrasting alterations to synaptic and intrinsic properties in upper-cervical superficial dorsal horn neurons following acute neck muscle inflammation', MOLECULAR PAIN, 10 (2014) [C1]
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Nova |
2014 |
Stuart DG, Schaefer AT, Massion J, Graham BA, Callister RJ, 'Pioneers in CNS inhibition: 1. Ivan M. Sechenov, the first to clearly demonstrate inhibition arising in the brain', Brain Research, 1548 20-48 (2014) [C1]
This article reviews the contributions of Ivan Michailovich Sechenov [1829-1905] to the neurophysiological concept of central inhibition. He first studied this concept in the frog... [more]
This article reviews the contributions of Ivan Michailovich Sechenov [1829-1905] to the neurophysiological concept of central inhibition. He first studied this concept in the frog and on himself. Later his trainees extended the study of central inhibition to other mammalian species. Outside his own country, Sechenov is better known for his prescient contributions to physiological psychology. In Russia, however, he is also revered as "the father of Russian physiology," because of his contributions to neurophysiology and other aspects of physiology including blood gases and respiration, the physiology and biomechanics of movement, and general physiology concepts that appeared in his textbooks and later works he helped translate from largely German sources. After graduation from Moscow University Medical School in 1856 he spent 31/2 years in Germany and Austria where he attended lectures and conducted research under the direction of several prominent physiologists and biochemists. In his subsequent academic career he held positions at universities in St. Petersburg (1860-1870; 1876-1888), Odessa (1871-1876) and Moscow (1890-1905). From 1860 onwards he was acclaimed as a physiologist in academic circles. He was also well known in Russian society for his public lectures on physiology and his views on physiological psychology. The latter resulted in him being branded "politically unreliable" by the tsarist bureaucracy from 1863 onwards. Sechenov's first (1862) study on central inhibition remains his most memorable. He delayed the withdrawal of a frog's foot from a weak acid solution by chemical or electrical stimulation of selected parts of the central nervous system. He also noted similar effects on his own hand during co-activation of other sensory inputs by tickling or teeth gnashing. © 2013 Elsevier B.V. All rights reserved.
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Nova |
2014 |
Smith KM, Madden JF, Callister RJ, Hughes DI, Graham BA, 'The search for novel analgesics: re-examining spinal cord circuits with new tools.', Front Pharmacol, 5 22 (2014) [C1]
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Nova |
2014 |
Pursey KM, Stanwell PT, Callister RJ, Brain K, Collins CE, Burrows TL, 'Neural responses to visual food cues according to weight status: a systematic review of functional magnetic resonance imaging studies', Frontiers in Nutrition, 1 1-11 (2014) [C1]
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Nova |
2013 |
Hughes DI, Boyle KA, Kinnon CM, Bilsland C, Quayle JA, Callister RJ, Graham BA, 'HCN4 subunit expression in fast-spiking interneurons of the rat spinal cord and hippocampus', Neuroscience, 237 7-18 (2013) [C1]
Hyperpolarisation-activated (Ih) currents are considered important for dendritic integration, synaptic transmission, setting membrane potential and rhythmic action potential (AP) ... [more]
Hyperpolarisation-activated (Ih) currents are considered important for dendritic integration, synaptic transmission, setting membrane potential and rhythmic action potential (AP) discharge in neurons of the central nervous system. Hyperpolarisation-activated cyclic nucleotide-gated (HCN) channels underlie these currents and are composed of homo- and hetero-tetramers of HCN channel subunits (HCN1-4), which confer distinct biophysical properties on the channel. Despite understanding the structure-function relationships of HCN channels with different subunit stoichiometry, our knowledge of their expression in defined neuronal populations remains limited. Recently, we have shown that HCN subunit expression is a feature of a specific population of dorsal horn interneurons that exhibit high-frequency AP discharge. Here we expand on this observation and use neuroanatomical markers to first identify well-characterised neuronal populations in the lumbar spinal cord and hippocampus and subsequently determine whether HCN4 expression correlates with high-frequency AP discharge in these populations. In the spinal cord, HCN4 is expressed in several putative inhibitory interneuron populations including parvalbumin (PV)-expressing islet cells (84.1%; SD: ±2.87), in addition to all putative Renshaw cells and Ia inhibitory interneurons. Similarly, virtually all PV-expressing cells in the hippocampal CA1 subfield (93.5%; ±3.40) and the dentate gyrus (90.9%; ±6.38) also express HCN4. This HCN4 expression profile in inhibitory interneurons mirrors both the prevalence of Ih sub-threshold currents and high-frequency AP discharge. Our findings indicate that HCN4 subunits are expressed in several populations of spinal and hippocampal interneurons, which are known to express both Ih sub-threshold currents and exhibit high-frequency AP discharge. As HCN channel function plays a critical role in pain perception, learning and memory, and sleep as well as the pathogenesis of several neurological diseases, these findings provide important insights into the identity and neurochemical status of cells that could underlie such conditions. © 2013 IBRO.
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Nova |
2013 |
Flynn JR, Dunn LR, Galea MP, Callister R, Callister RJ, Rank MM, 'Exercise Training after Spinal Cord Injury Selectively Alters Synaptic Properties in Neurons in Adult Mouse Spinal Cord', JOURNAL OF NEUROTRAUMA, 30 891-896 (2013) [C1]
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Nova |
2012 |
Tadros MA, Harris B, Anderson WB, Brichta AM, Graham BA, Callister RJ, 'Are all spinal segments equal: Intrinsic membrane properties of superficial dorsal horn neurons in the developing and mature mouse spinal cord', Journal of Physiology, 590 2409-2425 (2012) [C1]
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Nova |
2012 |
Hughes DI, Sikander S, Kinnon CM, Boyle KA, Watanabe M, Callister RJ, Graham BA, 'Morphological, neurochemical and electrophysiological features of parvalbumin-expressing cells: A likely source of axo-axonic inputs in the mouse spinal dorsal horn', Journal of Physiology, 590 3927-3951 (2012) [C1]
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Nova |
2012 |
Battistuzzo CR, Callister RJ, Callister R, Galea MP, 'A systematic review of exercise training to promote locomotor recovery in animal models of spinal cord injury', Journal of Neurotrauma, 29 1600-1613 (2012) [C1]
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Nova |
2012 |
De Oliveira R, Gravina FS, Lim R, Brichta AM, Callister RJ, Van Helden DF, 'Heterogeneous responses to antioxidants in noradrenergic neurons of the Locus coeruleus indicate differing susceptibility to free radical content', Oxidative Medicine and Cellular Longevity, 2012 820285 (2012) [C1]
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Nova |
2011 |
De Oliveira R, Gravina FS, Lim R, Brichta AM, Callister RJ, Van Helden DF, 'Developmental changes in pacemaker currents in mouse locus coeruleus neurons', Brain Research, 1425 27-36 (2011) [C1]
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Nova |
2011 |
Lim R, McPherson AE, Donne SW, Callister RJ, Brichta AM, 'Potassium accumulation between type I hair cells and calyx terminals in mouse crista', Experimental Brain Research, 210 607-621 (2011) [C1]
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Nova |
2011 |
Graham BA, Tadros MA, Schofield PR, Callister RJ, 'Probing glycine receptor stoichiometry in superficial dorsal horn neurones using the spasmodic mouse', Journal of Physiology, 589 2459-2474 (2011) [C1]
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Nova |
2011 |
Flynn JR, Graham BA, Galea MP, Callister RJ, 'The role of propriospinal interneurons in recovery from spinal cord injury', Neuropharmacology, 60 809-822 (2011) [C1]
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Nova |
2011 |
Pringle KG, Tadros MA, Callister RJ, Lumbers ER, 'The expression and localization of the human placental prorenin/renin-angiotensin system throughout pregnancy: Roles in trophoblast invasion and angiogenesis?', Placenta, 32 956-962 (2011) [C1]
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Nova |
2011 |
Flynn JR, Brichta AM, Galea MP, Callister RJ, Graham BA, 'A horizontal slice preparation for examining the functional connectivity of dorsal column fibres in mouse spinal cord', Journal of Neuroscience Methods, 200 113-120 (2011) [C1]
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Nova |
2010 |
Callister RJ, Graham BA, 'Early history of glycine receptor biology in mammalian spinal cord circuits', Frontiers in Molecular Neuroscience, 3 1-13 (2010) [C1]
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Nova |
2010 |
Jobling P, Graham BA, Brichta AM, Callister RJ, 'Cervix stimulation evokes predominantly subthreshold synaptic responses in mouse thoracolumbar and lumbosacral superficial dorsal horn neurons', Journal of Sexual Medicine, 7 2068-2076 (2010) [C1]
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Nova |
2010 |
Lim R, Camp AJ, Walsh MA, Callister RJ, Brichta AM, 'In vitro whole-cell conductances recorded from developing human cristae.', J Vestib Res, 285-286 (2010)
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2010 |
Lim R, Stitt IM, Camp AJ, Walsh MA, Callister RJ, Brichta AM, 'Inhibitory synaptic transmission in the lateral vestibular nucleus.', J. Vestib. Res, 286-287 (2010)
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2010 |
Lim R, Callister RJ, Brichta AM, 'An increase in glycinergic quantal amplitude and frequency during early vestibular compensation in mouse', Journal of Neurophysiology, 103 16-24 (2010) [C1]
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Nova |
2010 |
De Oliveira R, Graham BA, Howlett MC, Gravina FS, Oliveira MW, Imtiaz MS, et al., 'Ketamine anesthesia helps preserve neuronal viability', Journal of Neuroscience Methods, 189 230-232 (2010) [C1]
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Nova |
2010 |
Camp AJ, Lim R, Anderson WB, Schofield PR, Callister RJ, Brichta AM, 'Attenuated glycine receptor function reduces excitability of mouse medial vestibular nucleus neurons', Neuroscience, 170 348-360 (2010) [C1]
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Nova |
2010 |
De Oliveira R, Howlett MC, Gravina FS, Imtiaz MS, Callister RJ, Brichta AM, Van Helden DF, 'Pacemaker currents in mouse locus coeruleus neurons', Neuroscience, 170 166-177 (2010) [C1]
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Nova |
2009 |
Anderson WB, Graham BA, Beveridge NJ, Tooney PA, Brichta AM, Callister RJ, 'Different forms of glycine- and GABA(A)-receptor mediated inhibitory synaptic transmission in mouse superficial and deep dorsal horn neurons', Molecular Pain, 5 1-16 (2009) [C1]
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Nova |
2009 |
Tadros MA, Graham BA, Brichta AM, Callister RJ, 'Evidence for a critical period in the development of excitability and potassium currents in mouse lumbar superficial dorsal horn neurons', Journal of Neurophysiology, 101 1800-1812 (2009) [C1]
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Nova |
2008 |
Graham BA, Brichta AM, Callister RJ, 'Recording temperature affects the excitability of mouse superficial dorsal horn neurons, in vitro', Journal of Neurophysiology, 99 2048-2059 (2008) [C1]
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Nova |
2007 |
Graham BA, Brichta AM, Callister RJ, 'Moving from an averaged to specific view of spinal cord pain processing circuits', Journal of Neurophysiology, 98 1057-1063 (2007) [C1]
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Nova |
2007 |
Graham BA, Brichta AM, Callister RJ, 'Pinch-current injection defines two discharge profiles in mouse superficial dorsal horn neurones, in vitro', Journal of Physiology, 578 787-798 (2007) [C1]
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Nova |
2007 |
Graham BA, Brichta AM, Schofield PR, Callister RJ, 'Altered potassium channel function in the superficial dorsal horn of the spastic mouse', Journal of Physiology, 584 121-136 (2007) [C1]
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Nova |
2006 |
Graham BA, Schofield PR, Sah P, Margrie TW, Callister RJ, 'Distinct physiological mechanisms underlie altered glycinergic synaptic transmission in the murine mutants, spastic, spasmodic, and oscillator', Journal of Neuroscience, 26 4880-4890 (2006) [C1]
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Nova |
2006 |
Lynch JW, Callister RJ, 'Glycine receptors: A new therapeutic target in pain pathways', Current Opinion in Investigational Drugs, 7 48-53 (2006) [C1]
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2006 |
Camp AJ, Callister RJ, Brichta AM, 'Inhibitory synaptic transmission differs in mouse type A and B medial vestibular nucleus neurons in vitro', Journal of Neurophysiology, 95 3208-3218 (2006) [C1]
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Nova |
2005 |
Lee HY, Camp AJ, Callister RJ, Brichta AM, 'Vestibular primary afferent activity in an in vitro preparation of the mouse inner ear', Journal of Neuroscience Methods, 145 73-87 (2005) [C1]
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Nova |
2005 |
Callister RJ, Pierce PA, McDonagh JC, Stuart DG, 'Slow-tonic muscle fibers and their potential innervation in the turtle, Pseudemys (Trachemys) scripta elegans', Journal of Morphology, 264 62-74 (2005) [C1]
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2004 |
Graham BA, Brichta AM, Callister RJ, 'In vivo responses of mouse superficial dorsal horn neurones to both current injection and peripheral cutaneous stimulation', Journal of Physiology, 561.3 749-763 (2004) [C1]
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Nova |
2004 |
Callister RJ, Sesodia S, Enoka RM, Nemeth PM, Reinking RM, Stuart DG, 'Fatigue of Rat Hindlimb Motor Units: Biochemical -Physiological Associations', Muscle Nerve, 30 714-726 (2004) [C1]
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2004 |
Graham BA, Brichta AM, Callister RJ, 'An in vivo mouse spinal cord preparation for patch-clamp analysis of nociceptive processing', Journal of Neuroscience Methods, 136 221-228 (2004) [C1]
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2004 |
McDonagh J, Callister RJ, Favron M, Stuart D, 'Resistance to disuse atrophy in a turtle hindlimb muscle', Journal of Comparative Physiology A-Neuroethology Sensory Neural and Behavioural Physiology, 190(4) 321-329 (2004) [C1]
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2003 |
Graham BA, Schofield PR, Sah P, Callister RJ, 'Altered inhibitory synaptic transmission in superficial dorsal horn neurones in spastic and oscillator mice', The Journal of Physiology, 551.3 905-916 (2003) [C1]
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Nova |
2003 |
Callister RJ, Reinking RM, Stuart DG, 'Effects of fatigue on the catchlike property in a turtle hindlimb muscle', Journal of Comparative Physiology A, 189 857-866 (2003) [C1]
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2002 |
Camp AJ, Lee H, Callister RJ, Brichta AM, 'Afferent responses to mechanical stimulation and drug application in mouse in-vitro labyrinth', Journal of Vestibular Research, 11 175 (2002) [C3]
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2002 |
Brichta AM, Camp AJ, Lee H, Callister RJ, 'Intra-axonal recordings from canal afferents in the mouse in-vitro labyrinth', Journal of Vestibular Research, 11 177-178 (2002) [C3]
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2002 |
Graham BA, Schofield P, Sah P, Callister RJ, 'GABAAergic and glycinergic synaptic transmission in superficial dorsal horn neurones of wild type, spastic and oscillator mice', Proceedings of the Australian Neuroscience Society, 22:94 n/a (2002) [C3]
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2001 |
Faber ESL, Callister RJ, Sah P, 'Morphological and Electrophysiological Properties of Principal Neurons in the Rat Lateral Amygdala In Vitro', Journal of Neurophysiology, 85 714-723 (2001) [C1]
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Nova |
1999 |
Callister RJ, Schofield P, Sah P, 'The use of murine mutants to study glycine receptor function', Clinical and Experimental Pharmacology and Physiology., 26 929-931 (1999) [C1]
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1999 |
Callister RJ, Peterson E, Brichta AM, 'Neuromuscular strategies underlying ballistic movements', Progress in Brain Research, 123 233-243 (1999) [C1]
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1997 |
Callister RJ, Sah P, 'The removal of acetylcholine by diffusion at nicotinic synapses in the rat otic ganglion', JOURNAL OF PHYSIOLOGY-LONDON, 505 165-175 (1997)
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1997 |
Callister RJ, Keast JR, Sah P, 'Ca2+-activated K+ channels in rat otic ganglion cells: Role of Ca2+ entry via Ca2+ channels and nicotinic receptors', JOURNAL OF PHYSIOLOGY-LONDON, 500 571-582 (1997)
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1997 |
Callister RJ, Keast JR, Sah P, 'Ca
1. Intracellular recordings were made from neurones in the rat otic ganglion in vitro in order to investigate their morphological, physiological and synaptic properties. We took a... [more]
1. Intracellular recordings were made from neurones in the rat otic ganglion in vitro in order to investigate their morphological, physiological and synaptic properties. We took advantage of the simple structure of these cells to test for a possible role of calcium influx via nicotinic acetylcholine receptors during synaptic transmission. 2. Cells filled with biocytin comprised a homogeneous population with ovoid somata and sparse dendritic trees. Neurones had resting membrane potentials of -53 ± 0.7 mV (n = 69), input resistances of 112 ± 7 MO, and membrane time constants of 14 ± 0.9 ms (n = 60). Upon depolarization, all cells fired overshooting action potentials which were followed by an apamin-sensitive after-hyperpolarization (AHP). In response to a prolonged current injection, all neurones fired tonically. 3. The repolarization phase of action potentials had a calcium component which was mediated by N-type calcium channels. Application of ¿-conotoxin abolished both the repolarizing hump and the after-hyperpolarization suggesting that calcium influx via N-type channels activates SK-type calcium-activated potassium channels which underlie the AHP. The majority (70%) of neurones received innervation from a single preganglionic fibre which generated a suprathreshold excitatory postsynaptic potential mediated by nicotinic acetylcholine receptors. The other 30% of neurones also had one or more subthreshold nicotinic inputs. 5. Calcium influx via synaptic nicotinic receptors contributed to the BHP current, indicating that this calcium has access to the calcium-activated potassium channels and therefore plays a role in regulating cell excitability.
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1996 |
Callister RJ, Walmsley B, 'Amplitude and time course of evoked and spontaneous synaptic currents in rat submandibular ganglion cells', JOURNAL OF PHYSIOLOGY-LONDON, 490 149-157 (1996)
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1996 |
Callister RJ, Donnelly RP, Pierce PA, Stuart DG, 'Motor pool organization of the external gastrocnemius muscle in the turtle, Pseudemys (Trachemys) scripta elegans', JOURNAL OF MORPHOLOGY, 227 171-183 (1996)
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1995 |
LAIDLAW DH, CALLISTER RJ, STUART DG, 'FIBER-TYPE COMPOSITION OF HINDLIMB MUSCLES IN THE TURTLE, PSEUDEMYS (TRACHEMYS) SCRIPTA ELEGANS', JOURNAL OF MORPHOLOGY, 225 193-211 (1995)
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1995 |
CALLISTER RJ, LAIDLAW DH, STUART DG, 'A COMMENTARY ON THE SEGMENTAL MOTOR SYSTEM OF THE TURTLE - IMPLICATIONS FOR THE STUDY OF ITS CELLULAR MECHANISMS AND INTERACTIONS', JOURNAL OF MORPHOLOGY, 225 213-227 (1995)
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1992 |
CALLISTER RJ, CALLISTER R, PETERSON EH, 'DESIGN AND CONTROL OF THE HEAD RETRACTOR MUSCLE IN A TURTLE, PSEUDEMYS-(TRACHEMYS)-SCRIPTA .1. ARCHITECTURE AND HISTOCHEMISTRY OF SINGLE MUSCLE-FIBERS', JOURNAL OF COMPARATIVE NEUROLOGY, 325 405-421 (1992)
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1992 |
CALLISTER RJ, PETERSON EH, 'DESIGN AND CONTROL OF THE HEAD RETRACTOR MUSCLE IN A TURTLE, PSEUDEMYS-(TRACHEMYS)-SCRIPTA .2. EFFERENT INNERVATION', JOURNAL OF COMPARATIVE NEUROLOGY, 325 422-434 (1992)
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1991 |
CALLISTER R, CALLISTER RJ, STARON RS, FLECK SJ, TESCH P, DUDLEY GA, 'PHYSIOLOGICAL-CHARACTERISTICS OF ELITE JUDO ATHLETES', INTERNATIONAL JOURNAL OF SPORTS MEDICINE, 12 196-203 (1991)
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1990 |
CALLISTER R, CALLISTER RJ, FLECK SJ, DUDLEY GA, 'PHYSIOLOGICAL AND PERFORMANCE RESPONSES TO OVERTRAINING IN ELITE JUDO ATHLETES', MEDICINE AND SCIENCE IN SPORTS AND EXERCISE, 22 816-824 (1990)
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1989 |
CALLISTER RJ, CALLISTER R, PETERSON EH, 'HISTOCHEMICAL CLASSIFICATION OF NECK AND LIMB MUSCLE-FIBERS IN A TURTLE, PSEUDEMYS-SCRIPTA, A STUDY USING MICROPHOTOMETRY AND CLUSTER-ANALYSIS TECHNIQUES', JOURNAL OF MORPHOLOGY, 199 269-286 (1989)
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1987 |
Brichta AM, Callister RJ, Peterson EH, 'Quantitative analysis of cervical musculature in rats: Histochemical composition and motor pool organization', J. Comp. Neurol, 255 351-368 (1987) [C1]
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1987 |
CALLISTER RJ, BRICHTA AM, PETERSON EH, 'QUANTITATIVE-ANALYSIS OF CERVICAL MUSCULATURE IN RATS - HISTOCHEMICAL COMPOSITION AND MOTOR POOL ORGANIZATION .2. DEEP DORSAL MUSCLES', JOURNAL OF COMPARATIVE NEUROLOGY, 255 369-385 (1987)
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1986 |
CALLISTER RJ, CALLISTER R, PETERSON EH, 'FIBER TOPOGRAPHY IN A DUALLY INNERVATED NECK MUSCLE', AMERICAN ZOOLOGIST, 26 A82-A82 (1986)
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