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Career Summary

Biography

I am an electrophysiologist and use both in vitro and in vivo preparations in my experiments. My in vivo work allows the study of nerve cell properties and responses to “natural” stimuli, only possible using an intact preparation (an approach I pioneered during my PhD). My in vitro work permits detailed study of synaptic and intrinsic membrane properties in nerve cells using spinal cord or brain slice preparations. My in vitro studies also allows single channel analysis of individual receptor properties. All this work has been undertaken in mice so I can take advantage of the availability of mutant and transgenic animals for analyzing the role of specific receptor types (e.g. I have studied glycine receptor function in the mutant mice Spastic, Spasmodic, and Oscillator) or the properties of specific nerve cell types (e.g. my more recent work characterizing green fluorescent protein-expressing nerve cells in the spinal cords of transgenic mice). Because of this broad expertise I was invited to join the teaching faculty of the Australian Course in Advance Neuroscience (ACAN) in 2011. ACAN is recognized as “the pre-eminent course in the Asia-Pacific region for young scientists who would like to become experts in cellular neurophysiology”. Similarly my research expertise and standing in pain research has recently been acknowledged with an invitation for me to organize a topical workshop for the 2012 International Association for the Study of Pain World Congress (held biennially). This prestigious meeting brings together over 6,000 of the world’s leading professionals in the field of pain research and treatment to discuss current progress and new perspectives on pain management.

Qualifications

  • Doctor of Philosophy, University of Newcastle, 12/12/2006
  • Bachelor of Biomedical Sciences, University of Newcastle
  • Bachelor of Biomedical Sciences (Hons), University of Newcastle, 14/11/2001

Research

Research keywords

  • Neurophysiology
  • Pain
  • Spinal cord
  • Synaptic transmission

Research expertise

My research expertise is based around electrophysiological techniques that range from real-time monitoring of conformational changes in cell membrane associated protiens (single channel recording) through to systems-level analysis of neuronal processing (in vivo recordings). I have pioneered the approach of making patch-clamp recordings in mouse spinal cord under in vivo conditions to analyse pain processing mechanisms in the whole animal. I also routinely make in vitro recordings for mouse spinal cord slices, alowing phamacological analyses of siganl processing in the dorsal horn.

Fields of Research

CodeDescriptionPercentage
111600Medical Physiology55
110900Neurosciences35
170200Cognitive Science10

Memberships

Body relevant to professional practice.

  • Member - Australian Neuroscience Society (ANS)
  • Member - International Association for the Study of Pain (IASP)

Appointments

Fellowship
National Health & Medical Research Council (Australia)
01/01/2008 - 01/12/2011

Collaboration

Since establishing my laboratory at Newcastle I have developed research projects built around three research themes.

Theme 1: Targeted study of specific spinal nerve cell types involved in pain processing

Theme 2: Immune and nervous system interactions in painful inflammatory conditions

Theme 3: Drug-induced synaptic plasticity in the lateral hypothalamus

Each theme has attracted funding in its own right and I have established several local (Newcastle), national (Sydney, Adelaide), and international (Glasgow, Singapore) collaborations with researchers who have expertise in other, complimentary disciplines.

Administrative

Administrative expertise

My position is research-based.

Teaching

Teaching keywords

  • Biomedical Science
  • Neuroscience

Teaching expertise

I proide teaching into a number of coursees where the topics covered can be broadly grouped as relating to anatomy and physiology. I coordinate a course in Exercise Physiology and act as the sole lecturer in that course. Other areas of particular experitse include CNS function, synaptic transmission and pain.

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Highlighted Publications

For publications that are currently unpublished or in-press, details are shown in italics.

YearCitationAltmetricsLink
2004Graham BA, Brichta AM, Callister RJ, 'In vivo responses of mouse superficial dorsal horn neurones to both current injection and peripheral cutaneous stimulation', Journal of Physiology, 561.3 749-763 (2004) [C1]
2003Graham BA, Schofield PR, Sah P, Callister RJ, 'Altered inhibitory synaptic transmission in superficial dorsal horn neurones in spastic and oscillator mice', The Journal of Physiology, 551.3 905-916 (2003) [C1]
2006Graham BA, Schofield PR, Sah P, Margrie TW, Callister RJ, 'Distinct physiological mechanisms underlie altered glycinergic synaptic transmission in the murine mutants, spastic, spasmodic, and oscillator', Journal of Neuroscience, 26 4880-4890 (2006) [C1]
2007Graham BA, Brichta AM, Callister RJ, 'Pinch-current injection defines two discharge profiles in mouse superficial dorsal horn neurones, in vitro', Journal of Physiology, 578 787-798 (2007) [C1]
2007Graham BA, Brichta AM, Callister RJ, 'Moving from an averaged to specific view of spinal cord pain processing circuits', Journal of Neurophysiology, 98 1057-1063 (2007) [C1]
2007Graham BA, Brichta AM, Schofield PR, Callister RJ, 'Altered potassium channel function in the superficial dorsal horn of the spastic mouse', Journal of Physiology, 584 121-136 (2007) [C1]
2008Graham BA, Brichta AM, Callister RJ, 'Recording temperature affects the excitability of mouse superficial dorsal horn neurons, in vitro', Journal of Neurophysiology, 99 2048-2059 (2008) [C1]

Publications

For publications that are currently unpublished or in-press, details are shown in italics.

Click on a category title below to expand the list of citations for that specific category.

Chapter (1 outputs)

YearCitationAltmetricsLink
2012Graham BA, Callister RJ, 'Pain', The Mouse Nervous System, Academic Press, San Diego 589-606 (2012) [B1]

Co-authors: ROBERT CALLISTER

Journal article (35 outputs)

YearCitationAltmetricsLink
2015Callister RJ, Graham BA, 'Spicing up the gabapentionoids: Facilitating gabapentin entry in spinal pain circuits', Neuroscience Letters, 584 395-396 (2015)
2014Stuart DG, Schaefer AT, Massion J, Graham BA, Callister RJ, 'Pioneers in CNS inhibition: 1. Ivan M. Sechenov, the first to clearly demonstrate inhibition arising in the brain', BRAIN RESEARCH, 1548 20-48 (2014) [C1]
2014Yeoh JW, James MH, Graham BA, Dayas CV, 'Electrophysiological characteristics of paraventricular thalamic (PVT) neurons in response to cocaine and cocaine- and amphetamine-regulated transcript (CART)', FRONTIERS IN BEHAVIORAL NEUROSCIENCE, 8 (2014) [C1]
2014Yeoh JW, Campbell EJ, James MH, Graham BA, Dayas CV, 'Orexin antagonists for neuropsychiatric disease: progress and potential pitfalls.', Front Neurosci, 8 36 (2014) [C1]
2014Harris BM, Hughes DI, Bolton PS, Tadros MA, Callister RJ, Graham BA, 'Contrasting alterations to synaptic and intrinsic properties in upper-cervical superficial dorsal horn neurons following acute neck muscle inflammation', MOLECULAR PAIN, 10 (2014) [C1]
2014Farrell KE, Keely S, Graham BA, Callister R, Callister RJ, 'A Systematic Review of the Evidence for Central Nervous System Plasticity in Animal Models of Inflammatory-mediated Gastrointestinal Pain', INFLAMMATORY BOWEL DISEASES, 20 176-195 (2014) [C1]
2014Callister RJ, Graham BA, 'Spicing up the gabapentionoids: Facilitating gabapentin entry in spinal pain circuits', Neuroscience Letters, (2014)
2014Tadros MA, Farrell KE, Schofield PR, Brichta AM, Graham BA, Fuglevand AJ, Callister RJ, 'Intrinsic and synaptic homeostatic plasticity in motoneurons from mice with glycine receptor mutations.', J Neurophysiol, 111 1487-1498 (2014)
2014Farrell KE, Graham BA, Keely S, Callister RJ, 'Understanding the mechanisms underlying chronic pain in IBD: A new method for studying visceral inputs from the gastrointestinal tract', JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY, 29 142-142 (2014)
2014Stuart DG, Schaefer AT, Massion J, Graham BA, Callister RJ, 'Pioneers in CNS inhibition: 1. Ivan M. Sechenov, the first to clearly demonstrate inhibition arising in the brain', Brain Research, 1548 20-48 (2014)
2014Wellings TP, Graham BA, Camp AJ, Callister RJ, Brichta AM, Lim R, 'Calcium binding proteins subdivide medial vestibular nucleus neurons', J Vestib Res, 24 191-192 (2014)
2014Smith KM, Madden JF, Callister RJ, Hughes DI, Graham BA, 'The search for novel analgesics: re-examining spinal cord circuits with new tools.', Front Pharmacol, 5 22 (2014)
2013Hughes DI, Boyle KA, Kinnon CM, Bilsland C, Quayle JA, Callister RJ, Graham BA, 'HCN4 subunit expression in fast-spiking interneurons of the rat spinal cord and hippocampus', Neuroscience, 237 7-18 (2013) [C1]
2012Yeoh JW, James MH, Jobling P, Bains JS, Graham BA, Dayas CV, 'Cocaine potentiates excitatory drive in the perifornical/lateral hypothalamus', Journal of Physiology, 590 3677-3689 (2012) [C1]
2012Tadros MA, Harris B, Anderson WB, Brichta AM, Graham BA, Callister RJ, 'Are all spinal segments equal: Intrinsic membrane properties of superficial dorsal horn neurons in the developing and mature mouse spinal cord', Journal of Physiology, 590 2409-2425 (2012) [C1]
2012Hughes DI, Sikander S, Kinnon CM, Boyle KA, Watanabe M, Callister RJ, Graham BA, 'Morphological, neurochemical and electrophysiological features of parvalbumin-expressing cells: A likely source of axo-axonic inputs in the mouse spinal dorsal horn', Journal of Physiology, 590 3927-3951 (2012) [C1]

Co-authors: ROBERT CALLISTER

2012James MH, Yeoh JW, Graham B, Dayas C, 'Insights for Developing Pharmacological Treatments for Psychostimulant Relapse Targeting Hypothalamic Peptide Systems.', Journal of Addiction Research and Therapy, 01 1-14 (2012)

Co-authors: CHRISTOPHER DAYAS

2011Graham BA, Tadros MA, Schofield PR, Callister RJ, 'Probing glycine receptor stoichiometry in superficial dorsal horn neurones using the spasmodic mouse', Journal of Physiology, 589 2459-2474 (2011) [C1]
2011Flynn JR, Graham BA, Galea MP, Callister RJ, 'The role of propriospinal interneurons in recovery from spinal cord injury', Neuropharmacology, 60 809-822 (2011) [C1]
2011Flynn JR, Brichta AM, Galea MP, Callister RJ, Graham BA, 'A horizontal slice preparation for examining the functional connectivity of dorsal column fibres in mouse spinal cord', Journal of Neuroscience Methods, 200 113-120 (2011) [C1]
2010Jobling P, Graham BA, Brichta AM, Callister RJ, 'Cervix stimulation evokes predominantly subthreshold synaptic responses in mouse thoracolumbar and lumbosacral superficial dorsal horn neurons', Journal of Sexual Medicine, 7 2068-2076 (2010) [C1]
2010Callister RJ, Graham BA, 'Early history of glycine receptor biology in mammalian spinal cord circuits', Frontiers in Molecular Neuroscience, 3 1-13 (2010) [C1]
2010De Oliveira R, Graham BA, Howlett MC, Gravina FS, Oliveira MW, Imtiaz MS, et al., 'Ketamine anesthesia helps preserve neuronal viability', Journal of Neuroscience Methods, 189 230-232 (2010) [C1]
2010Graham BA, Clausen PD, Bolton PS, 'A descriptive study of the force and displacement profiles of the toggle-recoil spinal manipulative procedure (adjustment) as performed by chiropractors', Manual Therapy, 15 74-79 (2010) [C1]
2009Anderson WB, Graham BA, Beveridge NJ, Tooney PA, Brichta AM, Callister RJ, 'Different forms of glycine- and GABA(A)-receptor mediated inhibitory synaptic transmission in mouse superficial and deep dorsal horn neurons', Molecular Pain, 5 1-16 (2009) [C1]
2009Tadros MA, Graham BA, Brichta AM, Callister RJ, 'Evidence for a critical period in the development of excitability and potassium currents in mouse lumbar superficial dorsal horn neurons', Journal of Neurophysiology, 101 1800-1812 (2009) [C1]
2008Graham BA, Brichta AM, Callister RJ, 'Recording temperature affects the excitability of mouse superficial dorsal horn neurons, in vitro', Journal of Neurophysiology, 99 2048-2059 (2008) [C1]
2007Graham BA, Brichta AM, Schofield PR, Callister RJ, 'Altered potassium channel function in the superficial dorsal horn of the spastic mouse', Journal of Physiology, 584 121-136 (2007) [C1]
2007Graham BA, Brichta AM, Callister RJ, 'Pinch-current injection defines two discharge profiles in mouse superficial dorsal horn neurones, in vitro', Journal of Physiology, 578 787-798 (2007) [C1]
2007Graham BA, Brichta AM, Callister RJ, 'Moving from an averaged to specific view of spinal cord pain processing circuits', Journal of Neurophysiology, 98 1057-1063 (2007) [C1]
2006Graham BA, Schofield PR, Sah P, Margrie TW, Callister RJ, 'Distinct physiological mechanisms underlie altered glycinergic synaptic transmission in the murine mutants, spastic, spasmodic, and oscillator', Journal of Neuroscience, 26 4880-4890 (2006) [C1]
2004Graham BA, Brichta AM, Callister RJ, 'An in vivo mouse spinal cord preparation for patch-clamp analysis of nociceptive processing', Journal of Neuroscience Methods, 136 221-228 (2004) [C1]
2004Graham BA, Brichta AM, Callister RJ, 'In vivo responses of mouse superficial dorsal horn neurones to both current injection and peripheral cutaneous stimulation', Journal of Physiology, 561.3 749-763 (2004) [C1]
2003Graham BA, Schofield PR, Sah P, Callister RJ, 'Altered inhibitory synaptic transmission in superficial dorsal horn neurones in spastic and oscillator mice', The Journal of Physiology, 551.3 905-916 (2003) [C1]
2002Graham BA, Schofield P, Sah P, Callister RJ, 'GABAAergic and glycinergic synaptic transmission in superficial dorsal horn neurones of wild type, spastic and oscillator mice', Proceedings of the Australian Neuroscience Society, 22:94 n/a (2002) [C3]

Co-authors: ROBERT CALLISTER

Show 32 more

Conference (23 outputs)

YearCitationAltmetricsLink
2013Wellings TP, Callister RJ, Graham BA, Brichta AM, Lim R, 'Sensing balance: the role of central vestibular nuclei', University of Western Sydney Medical School, University of Western Sydney (2013) [E3]
2012Flynn JR, Callister RJ, Graham BA, 'Electrophysiological properties of identified long descending propriospinal neurons in mice', Abstracts. Australian Neuroscience Society 32nd Annual Meeting, Gold Coast, Queensland (2012) [E3]

Co-authors: ROBERT CALLISTER

2012Tadros MA, Lim R, Graham BA, Hughes DI, Brichta AM, Callister RJ, 'Excitability of human ventral horn neurons during early foetal development', Abstracts. Australian Neuroscience Society 32nd Annual Meeting, Gold Coast, Queensland (2012) [E3]
2012Farrell KE, Keely S, Graham BA, Minahan KL, Madden JF, Callister RJ, 'Spinal cord signalling in a mouse model of inflammatory bowel disease', Journal of Gastroenterology and Hepatology, Adelaide, SA (2012) [E3]
2012Jobling P, Madden JF, Graham BA, 'Whole cell patch clamp recordings from muscle spindle afferent neurons in intact dorsal root ganglia isolated from mouse', Abstracts. Australian Neuroscience Society 32nd Annual Meeting, Gold Coast, Queensland (2012) [E3]

Co-authors: PHILLIP JOBLING

2012Tadros MA, Jack R, Lim R, Graham BA, Brichta AM, Hughes DI, Callister RJ, 'Sensorimotor processing in the spinal cord of the developing human fetus', Society for Neuroscience, New Orleans, USA (2012)
2011Jobling P, Smith K, Madden J, Hickey LR, Graham BA, 'Characterisation of pain behaviour, spinal neurochemistry and glial populations in a mouse antigen-induced arthritis model', Posters. Australian Neuroscience Society 31st Annual Meeting, Auckland, New Zealand (2011) [E3]

Co-authors: PHILLIP JOBLING

2011Tadros MA, Lim R, Graham BA, Hughes DI, Brichta AM, Callister RJ, 'Excitability of human ventral horn neurons during early foetal development', Poster Abstracts. Australian Neuroscience Society Annual Meeting, Auckland, NZ (2011) [E3]
2011Graham BA, Sah P, Brichta AM, Callister RJ, Hughes DI, 'Neuroanatomical and neurochemical features of parvalbumin-expressing neurons in the mouse spinal dorsal horn', Posters. Australian Neuroscience Society 31st Annual Meeting, Auckland, New Zealand (2011) [E3]
2010Graham BA, Hughes DI, Lim R, Sah P, Brichta AM, Callister RJ, 'Characterization of calretinin expressing interneurons in the superficial dorsal horn of the mouse spinal cord', 13th World Congress on Pain: Abstracts, Montreal, QC (2010) [E3]
2010Hughes DI, Sah P, Callister RJ, Graham BA, 'Neuroanatomical and electrophysiological features of parvalbumin-expressing neurons in the rodent spinal dorsal horn', 13th World Congress on Pain: Abstracts, Montreal, QC (2010) [E3]

Co-authors: ROBERT CALLISTER

2010Harris BM, Graham BA, Bolton PS, Brichta AM, Callister RJ, 'Influence of acute neck muscle inflammation on the excitability of superficial dorsal horn neurons', 13th World Congress on Pain: Abstracts, Montreal, QC (2010) [E3]
2010Walsh MA, Farrell KE, Graham BA, Brichta AM, Callister RJ, 'Sodium current properties differ in neonate and adult superficial dorsal horn neurons', 13th World Congress on Pain: Abstracts, Montreal, QC (2010) [E3]
2010Callister RJ, Walsh MA, Harris BM, Anderson WB, Brichta AM, Graham BA, 'Segmental and developmental differences in the excitability of mouse superficial dorsal horn neurons', 13th World Congress on Pain: Abstracts, Montreal, QC (2010) [E3]
2009Graham BA, Schofield PR, Callister RJ, 'Glycine receptor mediated synaptic transmission in the superficial dorsal horn of Spasmodic mice', ANS 2009 Abstracts: Posters, Canberra, ACT (2009) [E3]

Co-authors: ROBERT CALLISTER

2009Tadros MA, Anderson WB, Graham BA, Callister RJ, 'Responses to current injection differ between mouse cervical, thoracic and lumbar superficial dorsal horn neurons', ANS 2009 Abstracts: Posters, Canberra, ACT (2009) [E3]

Co-authors: ROBERT CALLISTER

2008Callister RJ, Brichta AM, Graham BA, 'Beyond the dorsal horn: The use of animal models to discover new sites for pain therapy', Australian and New Zealand Journal of Psychiatry, Newcastle, NSW (2008) [E3]
2008Jobling P, Graham BA, Brichta AM, Callister RJ, 'In vivo patch clamp recording of synaptic events evoked in superficial dorsal horn neurons after stimulation of the female reproductive tract in the mouse', Proceedings of the Australian Neuroscience Society, Hobart, TAS (2008) [E3]
2006Jobling P, Graham BA, Brichta AM, Callister RJ, 'In vivo patch-clamp recording of subthreshold synaptic events evoked in dorsal horn neurons after stimulation of the female reproductive tract in the mouse', Society for Neuroscience, Atlanta, Georgia (2006) [E3]
2006Anderson WB, Graham BA, Jobling P, Lim R, Brichta AM, Callister RJ, 'Glycine receptor diversity in the dorsal horn of the mouse spinal cord', Society for Neuroscience, Atlanta, GA (2006) [E3]
2006Walsh MA, Graham BA, Brichta AM, Callister RJ, 'Postnatal development of electrophysiological properties in mouse supeficial dorsal horn neurones', Proceedings of the Australian Neuroscience Society, Sydney (2006) [E3]
2005Callister RJ, Graham BA, Brichta AM, 'In Vivo Responses of Mouse Spinal Neurones to Electrical and Functionally-Relevant Stimulation', Proceedings of the Australian Neuroscience Society Conference, Perth (2005) [E3]
2005Graham BA, Brichta AM, Callister RJ, 'Effect of Temperature on the Discharge Properties of Mouse Superficial Dorsal Horn Neurons', Proceedings of the Australian Neuroscience Society, Perth (2005) [E3]
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Research Supervision

Number of current supervisions7
Total current UoN PhD EFTSL2.83

For supervisions undertaken at an institution other that the University of Newcastle, the institution name is listed below the program name.

Current Supervision

CommencedProposed
Completion
ProgramSupervisor TypeResearch Title
20142018PhD (Human Physiology)Principal SupervisorThe Sinoatrial Node Pacemaker in Health and Disease
20142018PhD(Experimental Pharmacology)Co-SupervisorNeurobiological Investigation of Interstitial White Matter Neurons in a Maternal Infection Activation Model of Schizophrenia
20142018PhD (Anatomy)Co-SupervisorThe Role of Group III Metabotropic Glutamate Receptors in Psychostimulant Addiction
20132017PhD (Human Physiology)Principal SupervisorThe Role of Calretinin Positive Interneurons in Spinal Sensory Coding
20112015PhD (Anatomy)Co-SupervisorSpinal Cord Signalling in a Mouse Model of IBD
20102014PhD (Anatomy)Co-SupervisorUnderstanding Drug-Induced Changes to the Lateral Hypothalamic Orexin Circuitry
20102014PhD (Anatomy)Principal SupervisorElectrophysiological Investigation of Spinal Cord Injury and Characterisation of Propriospinal Neurons

Past Supervision

YearProgramSupervisor TypeResearch Title
2012M Philosophy (Anatomy)Co-SupervisorThe Effect of Neck Muscle Inflammation on Neuronal Excitability in the Dorsal Horn of the Spinal Cord
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Dr Brett Graham

Work Phone(02) 4921 5397
Fax(02) 4921 7906
Email
PositionSenior Lecturer
School of Biomedical Sciences and Pharmacy
Faculty of Health and Medicine
The University of Newcastle, Australia
Focus AreaAnatomy
Office
MS411,
Medical Sciences,
Callaghan
University Drive
Callaghan NSW 2308
Australia
URL:www.newcastle.edu.au/profile/brett-graham