Dr Jude Weidenhofer

Lecturer

School of Biomedical Sciences and Pharmacy (Medical Biochemistry)

Career Summary

Biography

I completed my PhD investigating the molecular and cellular role of the amygdala in the aetiology of schizophrenia in 2006 at the University of Newcastle. Following a post-doctoral position at the Children's Oncology Unit at the Children's Hospital at Westmead investigating the role of TPD52 in breast development and breast cancer I returned to the University of Newcastle in 2008. Whilst this position was as a teaching focused academic, I initiated my own research direction under the supervision of Prof. Leonie Ashman which resulted in successful funding from the Prostate Cancer Foundation of Australia. Since 2011 I have lead my own research group investigating the regulation of the expression of tetraspanin proteins in breast and prostate cancers as well as the role of CD151 in glomerular disease.

Research Expertise
My current research focus is on understanding the role of three tetraspanin proteins, CD151, CD9 and CD82 in breast and prostate cancers. These proteins show de-regulated expression in these cancers which is consistent with their proposed roles as tumour and metastasis enhancers (CD151) or suppressors (CD9 & CD82). Our aim is to identify the molecular mechanisms underlying their altered expression in cancer with a current focus on the role of miRNA and the ubiquitin system. We hope that by improving our understanding of how the expression of these proteins is controlled we can gain valuable insight into how they can be used as both prognostic indicators as well as novel therapeutic targets, particularly for metastatic disease. In addition we are investigating the role that CD151 plays in the development and maintenance of the glomerular filtration barrier in the kidney. In particular we aim to identify biomarkers of glomerular disease through analyzing mice in which CD151 expression has been lost and identifying the mechanisms involved in the development of and susceptibility to disease.

Teaching Expertise
My current teaching focus is on genetics, bioinformatics and cancer cell biology. I also have experience in DNA, transcription, translation, protein synthesis and function, protein analysis and molecular biology. I have experience teaching B.Biomed Sci, M. Pharmacy and B. Medicine.

Administrative Expertise
I have been on the organising committee for several symposiums and research seminar series as well as other research related events. I am currently the NSW State representative for the Australian Society for Biochemistry and Molecular Biology (ASBMB). In a teaching capacity I have co-ordinated several courses at the University of Newcastle.


Qualifications

  • Doctor of Philosophy, University of Newcastle

Keywords

  • Bioinformatics
  • Breast Cancer
  • Cancer Cell Biology
  • Gene & Protein expression regulation
  • Genetics
  • Molecular Biology
  • Prostate Cancer
  • Protein biochemistry
  • Tetraspanin proteins

Fields of Research

CodeDescriptionPercentage
060405Gene Expression (incl. Microarray and other genome-wide approaches)25
060802Animal Cell and Molecular Biology25
111299Oncology and Carcinogenesis not elsewhere classified50

Professional Experience

UON Appointment

DatesTitleOrganisation / Department
1/01/2015 - LecturerUniversity of Newcastle
School of Biomedical Sciences and Pharmacy
Australia
27/07/2013 - 28/07/2013Casual LecturerUniversity of Newcastle
School of Biomedical Sciences and Pharmacy
Australia

Academic appointment

DatesTitleOrganisation / Department
1/07/2006 - 1/05/2008Post-doctoral ResearcherThe Children's Hospital at Westmead
Molecular Oncogoly Group
Australia
1/01/2006 - Membership - Australian Society of Medical ResearchAustralian Society of Medical Research
Australia
1/01/2006 - Membership - Australian Society of Biochemistry and Molecular BiologyAustralian Society of Biochemistry and Molecular Biology
Australia

Professional appointment

DatesTitleOrganisation / Department
1/03/2012 - NSW State RepresentativeAustralian Society of Biochemistry and Molecular Biology
Australia

Invitations

Speaker

YearTitle / Rationale
2012Role and regulation of tetraspanins CD151 and CD9 in breast and prostate cancers
Organisation: Institut Andre Lwoff
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Publications

For publications that are currently unpublished or in-press, details are shown in italics.


Chapter (1 outputs)

YearCitationAltmetricsLink
2009Weidenhofer J, Byrne JA, 'Isolation of nucleic acids from hard tissues', Handbook of Nucleic Acid Purification, CRC PressI Llc, United States 427-448 (2009)

Journal article (14 outputs)

YearCitationAltmetricsLink
2014Vuong QV, Sadeqzadeh E, Hirun S, Goldsmith CD, Zammitt N, Bowyer MB, et al., 'Phenolic Compounds, Antioxidant and Anti-Cancer Properties of the Australian Maroon Bush Scaevola spinescens (Goodeniaceae)', Journal of Bioanalysis & Biomedicine, S12 (2014) [C1]
DOI10.4172/1948-593X.S12-002
Co-authorsC Scarlett, Vanquan Vuong, Rick Thorne, Michael Bowyer, Jennette Sakoff
2014Roselli S, Kahl RGS, Copeland BT, Naylor MJ, Weidenhofer J, Muller WJ, Ashman LK, 'Deletion of Cd151 reduces mammary tumorigenesis in the MMTV/PyMT mouse model', BMC Cancer, 14 (2014) [C1]

Background: Tetraspanins are transmembrane proteins that serve as scaffolds for multiprotein complexes containing, for example, integrins, growth factor receptors and matrix metalloproteases, and modify their functions in cell adhesion, migration and transmembrane signaling. CD151 is part of the tetraspanin family and it forms tight complexes with ß1 and ß4 integrins, both of which have been shown to be required for tumorigenesis and/or metastasis in transgenic mouse models of breast cancer. High levels of the tetraspanin CD151 have been linked to poor patient outcome in several human cancers including breast cancer. In addition, CD151 has been implicated as a promoter of tumor angiogenesis and metastasis in various model systems.Methods: Here we investigated the effect of Cd151 deletion on mammary tumorigenesis by crossing Cd151-deficient mice with a spontaneously metastasising transgenic model of breast cancer induced by the polyoma middle T antigen (PyMT) driven by the murine mammary tumor virus promoter (MMTV).Results: Cd151 deletion did not affect the normal development and differentiation of the mammary gland. While there was a trend towards delayed tumor onset in Cd151 -/- PyMT mice compared to Cd151 +/+ PyMT littermate controls, this result was only approaching significance (Log-rank test P-value =0.0536). Interestingly, Cd151 deletion resulted in significantly reduced numbers and size of primary tumors but did not appear to affect the number or size of metastases in the MMTV/PyMT mice. Intriguingly, no differences in the expression of markers of cell proliferation, apoptosis and blood vessel density was observed in the primary tumors.Conclusion: The findings from this study provide additional evidence that CD151 acts to enhance tumor formation initiated by a range of oncogenes and strongly support its relevance as a potential therapeutic target to delay breast cancer progression. © 2014 Roselli et al.; licensee BioMed Central Ltd.

DOI10.1186/1471-2407-14-509
Co-authorsLeonie Ashman
2014Bond DB, Brzozowski J, Skelding KA, Roselli SR, Weidenhofer J, 'Use of tetraspanins CD151 and CD9 as biomarkers for breast cancer', Breast Cancer Management, 3 123-126 (2014) [C3]
DOI10.2217/bmt.14.2
Co-authorsKathryn Skelding
2014Mate K, Riveros C, Weidenhofer J, Goldie B, Scott J, Moscato P, et al., 'Strategies for Enhancing Communication between Students, Academics and Researchers participating in Large-Scale Undergraduate Research Projects', International Journal of Innovation in Science and Mathematics Education, 22 14-29 (2014) [C1]
Co-authorsLiz Milward, Karen Mate, Pablo Moscato
2013Mate K, Sim A, Weidenhofer J, Milward L, Scott J, 'Investigation of the human disease osteogenesis imperfecta: A research-based introduction to concepts and skills in biomolecular analysis', Biochemistry & Molecular Biology Education, 41 103-109 (2013) [C1]
DOI10.1002/bmb.20672Author URL
CitationsScopus - 1
Co-authorsAlistair Sim, Karen Mate, Liz Milward
2012Tynan R, Weidenhofer JC, Hinwood M, Cairns MJ, Day TA, Walker FR, 'A comparative examination of the anti-inflammatory effects of SSRI and SNRI antidepressants on LPS stimulated microglia', Brain Behavior and Immunity, 26 469-479 (2012) [C1]
CitationsScopus - 54Web of Science - 49
Co-authorsRohan Walker, Murray Cairns, Trevor Day
2009Weidenhofer JC, Ashman LK, 'CD151 (CD151 molecule (Raph blood group))', Atlas of Genetics and Cytogenetics in Oncology and Haematology, - - (2009) [C1]
Co-authorsLeonie Ashman
2009Weidenhofer JC, Scott R, Tooney PA, 'Investigation of the expression of genes affecting cytomatrix active zone function in the amygdala in schizophrenia: Effects of antipsychotic drugs', Journal of Psychiatric Research, 43 282-290 (2009) [C1]
DOI10.1016/j.jpsychires.2008.04.001
CitationsScopus - 6Web of Science - 6
Co-authorsRodney Scott, Paul Tooney
2008Shehata M, Bieche I, Boutros R, Weidenhofer J, Fanayan S, Spalding L, et al., 'Nonredundant Functions for Tumor Protein D52-Like Proteins Support Specific Targeting of TPD52', Clinical Cancer Research, 14 5050-5060 (2008) [C1]
DOI10.1158/1078-0432.CCR-07-4994
CitationsScopus - 18Web of Science - 16
2008Shehata M, Weidenhofer J, Thamotharampillai K, Byrne JA, 'Tumor protein D52 overexpression and gene amplification in cancer - from a mosaic of microarrays', Critical Reviews in Oncogenesis, 14 33-55 (2008) [C1]
CitationsScopus - 11
2006Weidenhofer JC, Bowden NA, Scott R, Tooney PA, 'Altered gene expression in the amygdala in schizophrenia: Up-regulation of genes located in the cytomatrix active zone', Molecular and Cellular Neuroscience, 31 243-250 (2006) [C1]
DOI10.1016/j.mcn.2005.09.013
CitationsScopus - 34Web of Science - 34
Co-authorsNikola Bowden, Paul Tooney, Rodney Scott
2006Weidenhofer JC, Yip JR, Zavitsanou K, Huang XF, Chahl LA, Tooney PA, 'Immunohistochemical localisation of the NK1 receptor in the human amygdala: Preliminary investigation in schizophrenia', Progress in Neuro-Psychopharmacology and Biological Psychiatry, 30 1313-1321 (2006) [C1]
DOI10.1016/j.pnpbp.2006.05.008
CitationsScopus - 6Web of Science - 5
Co-authorsPaul Tooney
2006Bowden NA, Weidenhofer JC, Scott R, Schall U, Todd J, Michie PT, Tooney PA, 'Preliminary investigation of gene expression profiles in peripheral blood lymphocytes in schizophrenia', Schizophrenia Research, 82 175-183 (2006) [C1]
DOI10.1016/j.schres.2005.11.012
CitationsScopus - 77Web of Science - 67
Co-authorsUlrich Schall, Nikola Bowden, Rodney Scott, Juanita Todd, Pat Michie, Paul Tooney
2006Weidenhofer J, Bowden NA, Scott RJ, Tooney PA, 'Dysfunction of genes regulating membrane exocytosis in schizophrenia', AUSTRALIAN AND NEW ZEALAND JOURNAL OF PSYCHIATRY, 40 A129-A129 (2006)
Author URL
Co-authorsPaul Tooney, Rodney Scott, Nikola Bowden
Show 11 more journal articles

Conference (13 outputs)

YearCitationAltmetricsLink
2014Bond DR, Passfield C, Cairns M, Ashman LK, Weidenhofer J, 'Posttranscriptional regulation of tetraspanins CD151 & CD9 in breast & prostate cancers', CANCER RESEARCH, San Diego, CA (2014) [E3]
DOI10.1158/1538-7445.AM2014-4364Author URL
Co-authorsLeonie Ashman, Murray Cairns
2014Sadeqzadeh E, Vuong QV, Goldsmith CD, Nguyen VT, Bhuyan DJ, Trung TD, et al., 'A NATURAL PRODUCT DRUG DISCOVERY PIPELINE FOR NOVEL PANCREATIC CANCER THERAPIES: A NEW CANCER RESEARCH HUB FOR THE HUNTER REGION OF NSW', ASIA-PACIFIC JOURNAL OF CLINICAL ONCOLOGY (2014) [E3]
Author URL
Co-authorsTroy Gaston, Vanquan Vuong, Michael Bowyer, Rick Thorne, C Scarlett, Natalie Moltschaniwskyj, Anita Chalmers
2013Weidenhofer J, Bond D, Copeland B, Ashman L, 'Tetraspanin protein turnover rates vary in prostate cancer cell lines', BJU INTERNATIONAL (2013) [E3]
Author URL
Co-authorsLeonie Ashman
2013Bond D, Cairns M, Ashman L, Weidenhofer J, 'Post-transcriptional regulation of CD151 & CD9 by miRNAs in prostate cancer', BJU INTERNATIONAL (2013) [E3]
Author URL
Co-authorsMurray Cairns, Leonie Ashman
2012Bond DR, Cairns MJ, Ashman LK, Weidenhofer JC, 'Investigating micro-RNA regulation of tetraspanins CD151 and CD9 in prostate cancer', Febs Journal, Seville, Spain (2012) [E3]
Co-authorsLeonie Ashman, Murray Cairns
2011Naudin C, Weidenhofer JC, Scott R, Ashman LK, Roselli SM, 'Induction of mindin expression is associated with glomerular basement membrane damage in Cd151(-/-) mice', Nephrology, Adelaide, SA (2011) [E3]
Co-authorsRodney Scott, Leonie Ashman
2006Weidenhofer JC, Bowden NA, Scott R, Tooney PA, 'Dysfunction of genes regulating membrane exocytosis in schizophrenia (Poster presentation)', Australian and New Zealand Journal of Psychiatry (Vol 40, noS2), Fremantle, Western Australia (2006) [E3]
Co-authorsNikola Bowden, Rodney Scott, Paul Tooney
2005Bowden NA, Weidenhofer JC, Scott R, Schall UA, Todd J, Michie PT, Tooney PA, 'Classification of schizophrenia using differential gene expression in peripheral blood lymphocytes', Human Genetics Society of Australasia, Newcastle (2005) [E3]
Co-authorsPat Michie, Rodney Scott, Ulrich Schall, Nikola Bowden, Juanita Todd, Paul Tooney
2004Bowden NA, Weidenhofer JC, Scott R, Todd J, Case V, Schall UA, Tooney PA, 'Altered Expression of Brain Related Genes in Lymphocytes in Schizophrenia', American Journal of Medical Genetics, Ireland (2004) [E3]
DOI10.1002/ajmg.b.30101
Co-authorsUlrich Schall, Nikola Bowden, Paul Tooney, Rodney Scott, Juanita Todd
2004Weidenhofer JC, Bowden NA, Scott R, Tooney PA, 'Gene Profiling in the Amygdala in Schizophrenia', American Journal of Medical Genetics, Ireland (2004) [E3]
Co-authorsPaul Tooney, Rodney Scott, Nikola Bowden
2004Bowden NA, Weidenhofer JC, Scott R, Todd J, Case V, Schall UA, Tooney PA, 'Differental Gene Expression in Peripheral Blood Lymphocytes in Schizophrenia', Proceedings of the Australian Neuroscience Society, Melbourne (2004) [E3]
Co-authorsPaul Tooney, Rodney Scott, Juanita Todd, Ulrich Schall, Nikola Bowden
2004Weidenhofer JC, Bowden NA, Scott R, Tooney PA, 'Altered Gene Expression Profiles in the Amygdala in Schizophrenia', Proceedings of the Australian Neuroscience Society, Melbourne (2004) [E3]
Co-authorsPaul Tooney, Rodney Scott, Nikola Bowden
2004Bowden NA, Weidenhofer JC, Scott R, Todd J, Case V, Schall UA, Tooney PA, 'Distinct Gene Expression Profiles due to Age in Schizophrenia', Proceedings of the Australian Neuroscience Society, Melbourne (2004) [E3]
Co-authorsNikola Bowden, Ulrich Schall, Rodney Scott, Paul Tooney, Juanita Todd
Show 10 more conferences
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Grants and Funding

Summary

Number of grants13
Total funding$1,202,146

Click on a grant title below to expand the full details for that specific grant.


20144 grants / $378,000

Tetraspanin CD9; more than just an exosome marker - A novel biomarker to target for prostate cancer$300,000

Funding body: Hunter Medical Research Institute

Funding bodyHunter Medical Research Institute
Project TeamDoctor Jude Weidenhofer, Doctor Kathryn Skelding, Doctor Matt Dun, Ms Belinda Goldie, Miss Danielle Bond
SchemeProject Grant
RoleLead
Funding Start2014
Funding Finish2014
GNoG1400921
Type Of FundingGrant - Aust Non Government
Category3AFG
UONY

Developing Synthetic Exosomes to Target and Deliver Anti-Cancer Agents to Prostate Cancer Cells$37,500

Funding body: Hunter Medical Research Institute

Funding bodyHunter Medical Research Institute
Project TeamMr Joshua Brzozowski, Doctor Jude Weidenhofer, Doctor Kathryn Skelding
SchemePostgraduate Research Scholarship
RoleLead
Funding Start2014
Funding Finish2014
GNoG1401405
Type Of FundingGrant - Aust Non Government
Category3AFG
UONY

Identification and evaluation of anti-pancreatic cancer activity of cytotoxic compounds extracted from Australian sea sponges: a pilot study$20,500

Funding body: Hunter Medical Research Institute

Funding bodyHunter Medical Research Institute
Project TeamDoctor Christopher Scarlett, Doctor Quan Vuong, Doctor Jude Weidenhofer, Doctor Rick Thorne, Associate Professor Michael Bowyer, Doctor Troy Gaston
SchemeProject Grant
RoleInvestigator
Funding Start2014
Funding Finish2014
GNoG1401452
Type Of FundingGrant - Aust Non Government
Category3AFG
UONY

A new frontier in breast cancer: Can small molecules in the blood predict outcome?$20,000

Funding body: Hunter Medical Research Institute

Funding bodyHunter Medical Research Institute
Project TeamDoctor Kelly Kiejda, Doctor Jude Weidenhofer, Professor Rodney Scott
SchemeProject Grant
RoleInvestigator
Funding Start2014
Funding Finish2014
GNoG1401454
Type Of FundingGrant - Aust Non Government
Category3AFG
UONY

20131 grants / $24,596

20124 grants / $355,550

Microscopic illumination system for advanced fluorescent protein technology$34,000

Funding body: NHMRC (National Health & Medical Research Council)

Funding bodyNHMRC (National Health & Medical Research Council)
Project TeamDoctor Rick Thorne, Professor Xu Dong Zhang, Conjoint Associate Professor Murray Cairns, Doctor Nikki Verrills, Doctor Charles De Bock, Doctor Jude Weidenhofer, Doctor Severine Roselli, Doctor Kathryn Skelding, Emeritus Professor Leonie Ashman, Professor Hubert Hondermarck
SchemeEquipment Grant
RoleInvestigator
Funding Start2012
Funding Finish2012
GNoG1100983
Type Of FundingOther Public Sector - Commonwealth
Category2OPC
UONY

Does Ubiquitination control the altered expression of tetraspanin proteins in Breast Cancer?$25,000

Funding body: Hunter Medical Research Institute

Funding bodyHunter Medical Research Institute
Project TeamDoctor Jude Weidenhofer, Emeritus Professor Leonie Ashman, Professor Hubert Hondermarck
SchemeProject Grant
RoleLead
Funding Start2012
Funding Finish2012
GNoG1200169
Type Of FundingContract - Aust Non Government
Category3AFC
UONY

PULSE - Education Prize$4,250

Funding body: Hunter Medical Research Institute

Funding bodyHunter Medical Research Institute
Project TeamDoctor Jude Weidenhofer
SchemePULSE Education Prize
RoleLead
Funding Start2012
Funding Finish2012
GNoG1200157
Type Of FundingScheme excluded from IGS
CategoryEXCL
UONY

20113 grants / $410,000

Identifying the mechanisms underlying altered expression and mode of action of tetraspanins CD151 and CD9 in metastasis: progression to targeted treatment for prostate cancer$375,000

Funding body: Prostate Cancer Foundation of Australia

Funding bodyProstate Cancer Foundation of Australia
Project TeamEmeritus Professor Leonie Ashman, Doctor Jude Weidenhofer
SchemeProject Grant
RoleLead
Funding Start2011
Funding Finish2011
GNoG1000863
Type Of FundingAust Competitive - Non Commonwealth
Category1NS
UONY

Investigation of the function of CD151 in the glomerular filtration barrier and identification of genetic modifiers of glomerular diseases$25,000

Funding body: University of Newcastle

Funding bodyUniversity of Newcastle
Project TeamDoctor Severine Roselli, Doctor Jude Weidenhofer, Emeritus Professor Leonie Ashman
SchemeNear Miss Grant
RoleInvestigator
Funding Start2011
Funding Finish2011
GNoG1001053
Type Of FundingInternal
CategoryINTE
UONY

IMPLEN NanoPhotometer pearl$10,000

Funding body: NHMRC (National Health & Medical Research Council)

Funding bodyNHMRC (National Health & Medical Research Council)
Project TeamConjoint Associate Professor Murray Cairns, Associate Professor Paul Tooney, Professor Alan Brichta, Emeritus Professor John Rostas, Emeritus Professor Patricia Michie, Conjoint Professor Keith Jones, Professor Ulli Schall, Associate Professor Phillip Dickson, Doctor Frederick Walker, Doctor Rick Thorne, Doctor Chris Dayas, Doctor Nikki Verrills, Doctor Janet Holt, Doctor Severine Roselli, Doctor Kathryn Skelding, Doctor Jude Weidenhofer, Associate Professor Liz Milward, Doctor Charles De Bock, Doctor Julie Merriman-Jones, Doctor Jing Qin Wu, Doctor Bing Liu, Mr Dan Johnstone, Ms Belinda Goldie, Doctor Natalie Beveridge
SchemeEquipment Grant
RoleInvestigator
Funding Start2011
Funding Finish2011
GNoG1100030
Type Of FundingOther Public Sector - Commonwealth
Category2OPC
UONY

20101 grants / $34,000

ABI 7500 Real Time PCR System $34,000

Funding body: NHMRC (National Health & Medical Research Council)

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Research Supervision

Current Supervision

CommencedResearch Title / Program / Supervisor Type
2014Identification of Biomarkers and Novel Targets for Prostate Cancer from Exosomes
Medical Science, Faculty of Health and Medicine
Principal Supervisor
2014Developing Synthetic Exosomes to Target and Deliver Anti-Cancer Agents to Prostate Cancer Cells
Medical Science, Faculty of Health and Medicine
Principal Supervisor
2011The Tetraspanin CD151's Role in the Kidney and Mapping of Genetic Modifiers of Glomerular Diseases
Medical Science, Faculty of Health and Medicine
Principal Supervisor
2011Post-Transcriptional Regulation of Tetraspanins CD151 and CD9 by Micro-RNA's in Prostate and Breast Cancers
Medical Science, Faculty of Health and Medicine
Principal Supervisor

Past Supervision

YearResearch Title / Program / Supervisor Type
2013Tetraspanins as Biomarkers and Causative Proteins in Prostate Cancer
Medical Science, Faculty of Health and Medicine
Co-Supervisor
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Dr Jude Weidenhofer

Position

Lecturer
School of Biomedical Sciences and Pharmacy
Faculty of Health and Medicine

Focus area

Medical Biochemistry

Contact Details

Emailjudith.weidenhofer@newcastle.edu.au
Phone(02) 4921 8706
Fax(02) 43494538

Office

RoomBE136
BuildingHealth Precinct
LocationOurimbah
10 Chittaway Road
Ourimbah, NSW 2258
Australia
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