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Emeritus Professor John Rostas

Emeritus Professor

Faculty of Health and Medicine

Career Summary

Biography

I have a distinguished research record in the study of molecular mechanisms of synaptic plasticity in many experimental systems, particularly in studies of brain development and maturation. I have always maintained a multidisciplinary approach in my research and active collaborations with colleagues in different disciplines both in Australia and overseas.

I have an international reputation in two main areas: 1. I am an internationally recognised authority on the role and regulation of the important regulatory enzyme CaMKII, as evidenced by my invitation to be a keynote speaker on this topic in an international symposium at the joint meeting of the International and European Societies for Neurochemistry in Innsbruck, Austria (August, 2005). 2. I am internationally recognised for developing the maturing chicken forebrain as an animal model for the slow maturation of brain observed in humans and for using biochemical, electrophysiological, anatomical and behavioural techniques in chickens to investigate the fundamental biological changes that occur during maturation of the brain.

Evidence for my recognition is an invitation to be a major speaker, and to chair a session, at the forthcoming Brain Mechanisms, Cognition and Behaviour in Birds Satellite Conference to the IBRO (International Brain Research Organisation) World Congress of Neuroscience (July 2007). I am also an invited Editor for the special issue of Brain Research Bulletin that will publish papers from this conference.

When comparing my scientific research track record against those of others, it must be noted that I have always performed my research while holding full academic roles that required major educational and administrative input. The extent of my activities in education and curriculum development is reflected by two peer reviewed publications in the area and invitations to speak at international conferences on problem based learning.

In the last 10 years I have continuously held senior academic administrative responsibilities (Assistant and Deputy Dean of the Faculty of Medicine and Health Sciences, Executive Director of the Hunter Medical Research Institute, Deputy Head of Faculty of Health) and been appointed to State and Commonwealth government advisory panels and chairmanships, including 9 years on the NHMRC Project Grants Committee.

Despite these responsibilities I have continued to publish 3-5 papers per year in high quality, international peer reviewed journals (~50% of papers appeared in journals with an impact factor above 4.8 and 70% above 3). In all, I have published more than 95 papers in high ranking international journals. The fact that much of my research has been performed in chickens accounts for the lower citation rate compared with similar work performed in mammals. My research papers have had a consistent citation rate of 70-90 per year for the last decade. I have received multiple invitations to speak at international and national conferences including the inaugural Lawrie Austin Plenary Lecture at the 2002 meeting of the Australian Neuroscience Society.

Research Expertise
I have a distinguished research record in the study of molecular mechanisms of synaptic plasticity in many experimental systems, particularly in studies of brain development and maturation. I have always maintained a multidisciplinary approach to research and active collaborations with colleagues in different disciplines both in Australia and overseas.

My research expertise is focused in two areas: 1. I am an internationally recognised authority on the role and regulation of the important regulatory enzyme CaMKII. In this field work from my laboratory was the first to show : " That developmental changes in subcellular distribution of CaMKII occur independently of the developmental change in expression level of the enzyme and that the subcellular translocation is indirectly regulated by thyroid hormone " The existence of giant mossy fibre nerve terminals in the chicken hippocampus and of LTP and LTD at these synapses, and to show that CaMKII activation is required for presynaptic LTD at these synapses. " That CaMKII regulation in vivo cannot be explained by currently known mechanisms and that there is a new class of regulatory mechanism operating in vivo. This new class of control mechanisms apparently occur only a specific functionally important locations in neurons (e.g. at the PSD in association with certain receptors) and require interaction with specific local proteins. If this is true, these proteins will provide highly selective drug targets through which the activity of CaMKII could be altered at specific locations in the neuron without altering CaMKII activity elsewhere in neurons or in other cells. This could lead to neuro/psycho-active drugs with fewer side-effects. These activation of these new control mechanisms could also be used as biomarkers of disease or neuropathology. 2. I am internationally recognised for developing the maturing chicken forebrain as an animal model for the slow maturation of brain observed in humans. Work from my laboratory has demonstrated the protracted maturation of neuronal function in chicken brain using biochemical, electrophysiological and anatomical techniques and identified some fundamental changes in biological mechanisms during maturation. The chicken model for brain maturation may provide opportunities to identify the major biological factors controlling the maturation process and to test a variety of drugs or other interventions on the normal progress of this maturation. This is of relevance to a variety of neurodevelopmental disorders.

Teaching Expertise
Supervised 14 postgraduate research students and 7 postdoctoral research fellows. Developed and taught integrated, problem based curricula for Bachelor of Medicine and Bachelor of Biomedical Science. Developed training programs for research planning and research grant preparation for postgraduate research students and university and hospital staff. Invited to speak on curriculum design and evaluation at major international centres in Europe and North America. Peer reviewed publications on problem based curriculum design: Rostas, J.A.P. and Olson,L.G. (1997). Teaching basic sciences in an integrated curriculum: making it work. In: Imperatives in Medical Education: The Newcastle Approach. Eds. R. Henry, K. Byrne and C. Engel. University of Newcastle, Australia, pp 83-94. Rostas, J.A.P. and Rolfe, I. (1997). Training working problem tutors for medical students. In: Imperatives in Medical Education: The Newcastle Approach, Eds. R. Henry, K. Byrne and C. Engel. University of Newcastle, Australia, pp 189-199. External Examiner for University of Papua New Guinea Medical School (1996), Royal Australian College of Obstetricians and Gynaecologists (1989-1994), and PhD theses for Sydney, Melbourne, Monash, New England and RMIT Universities.

Administrative Expertise
SELECTED SENIOR ADMINISTRATIVE APPOINTMENTS (since 1992) 2006 - Deputy Head of Faculty (Research), and Professor of Biomedical Sciences, Faculty of Health, University of Newcastle - responsible for coordinating research and research training across the Faculty at all campuses 1999 - 2006 Founding Executive Director, Hunter Medical Research Institute and Professor of Biomedical Sciences, Faculty of Health, The University of Newcastle - established the scientific organisation and governance foundations of HMRI, its recognition by the NSW Government as the third largest medical research institute in the state, and its infrastructure funding by NSW government on an equal footing with Sydney institutes. 1997 - 2001 Assistant Dean (Research), Faculty of Medicine & Health Sciences, University of Newcastle Chairmanship or Sole Responsibility 2001 - 2006 Chair, Complaints Subcommittee of NH&MRC Projects Grants Committee 2003 - 2006 Deputy Chair, NH&MRC Project Grants Committee 2003 - 2004 Chair, BioFirst High Bandwidth Network Implementation Committee, NSW Government 2002 - 2003 Chair, BioFirst High Bandwidth Network Committee, NSW Government 1997 - 2001 Assistant Dean (Research) and Chair of the Faculty Research Management Committee, Faculty of Medicine and Health Sciences, University of Newcastle 1996 - 1997 Chair, Local Organising Committee for 17th Annual Conference of the Australian Neuroscience Society 1992 - 1995 Chair, International Society for Neurochemistry Committee for Aid for Neurochemistry 1984 - 1995 Editor and member of Council Executive for Australian Neuroscience Society 2006 - Deputy Chair, Research Training Committee of The University of Newcastle Committee Membership 1997 - 2006 Project Grants Committee, National Health and Medical Research Council of Australia 2003 - 2006 Ministerial Advisory Committee on Medical and Health Research, NSW Government 2001 - 2006 Ministerial Advisory Committee on Biotechnology, NSW Government 2001 - 2006 BioFirst Awards Committee, NSW Government 1999 - 2004 Research and Development Advisory Committee, NSW Department of Health 1998 - 2006 Council of Asian Pacific Society for Neurochemistry 2003 - 2005 Cancer Research Advisory Board, Cancer Institute of NSW 1991 - 1995 Council of the International Society for Neurochemistry 1999 - 2004 Reference Group for Medical Research, Australian Business


Qualifications

  • PhD, Monash University
  • Bachelor of Science (Honours), Monash University

Keywords

  • biochemistry
  • cell biology
  • neuroscience

Fields of Research

Code Description Percentage
110199 Medical Biochemistry and Metabolomics not elsewhere classified 40
170299 Cognitive Sciences not elsewhere classified 20
110999 Neurosciences not elsewhere classified 40

Professional Experience

Academic appointment

Dates Title Organisation / Department
1/01/2003 - 1/08/2006 Ministerial Advisory Committee on Medical and Health Research NSW Government
Australia
1/09/1999 - 1/08/2006 Executive Director of Hunter Medical Research Institute University of Newcastle
Australia
1/09/1991 - 1/09/1995 Council of the International Society for Neurochemistry and Chair of its Committee for Aid for Neurochemistry International Society for Neurochemistry
United Kingdom

Membership

Dates Title Organisation / Department
1/01/2006 - 31/12/2006 Chair of Grant Review Panel - Molecular and Cellular Neuroscience NHMRC Committee
Australia
1/01/1995 -  member - Neurochemical Research (scientific journal) Neurochemical Research
Australia

Invitations

Participant

Year Title / Rationale
2005 Keynote speaker and Symposium Co-Chair
Organisation: International Society for Neurochemistry Description: Our proposal for a Symposium at the biennial conference (held jointly with the European Society for Neurochemistry) was chosen for inclusion in the final program from a field of more than 200 proposals. The four speakers came from UK, Netherlands, USA and Australia (me).
2002 Lawrie Austin Plenary Lecture
Organisation: Australian Neuroscience Society Description: It is an honour to be invited by the ANS Council to present this plenary lecture named after one of the founders of the Scociety. I was the first recipient of this honour which was launched on the occasion of the joint meeting of ANS with the International Society for Developmental Neuroscience in Sydney.
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Publications

For publications that are currently unpublished or in-press, details are shown in italics.


Chapter (2 outputs)

Year Citation Altmetrics Link
2012 Skelding KA, Rostas JA, 'The role of molecular regulation and targeting in regulating calcium/calmodulin stimulated protein kinases', Calcium Signaling, Springer New York LLC, New York, NY 703-730 (2012) [B1]
Citations Scopus - 5Web of Science - 5
Co-authors Kathryn Skelding
2010 Atkinson RJ, Rosso OA, Figliola A, Serrano E, Moscato PA, Hunter M, Rostas JA, 'Use of the domestic chicken to investigate mechanisms of brain maturation', Translational Neuroscience and Its Advancement of Animal Research Ethics, Nova Science Publishers, Hauppauge 29-53 (2010) [B1]
Co-authors Pablo Moscato, Mick Hunter

Journal article (143 outputs)

Year Citation Altmetrics Link
2014 Skelding KA, Arellano JM, Powis DA, Rostas JA, 'Excitotoxic stimulation of brain microslices as an in vitro model of stroke.', J Vis Exp, e51291 (2014) [C1]
DOI 10.3791/51291
Co-authors Kathryn Skelding
2014 Hoffman A, Carpenter H, Kahl R, Watt LF, Dickson PW, Rostas JAP, et al., 'Dephosphorylation of CaMKII at T253 controls the metaphase-anaphase transition', Cellular Signalling, 26 748-756 (2014) [C1]

Calcium/calmodulin-stimulated protein kinase II (CaMKII) is a multi-functional serine/threonine protein kinase that controls a range of cellular functions, including proliferation... [more]

Calcium/calmodulin-stimulated protein kinase II (CaMKII) is a multi-functional serine/threonine protein kinase that controls a range of cellular functions, including proliferation. The biological properties of CaMKII are regulated by multi-site phosphorylation and targeting via interactions with specific proteins. To investigate the role specific CaMKII phosphorylation sites play in controlling cell proliferation and cell cycle progression, we examined phosphorylation of CaMKII at two sites (T253 and T286) at various stages of the cell cycle, and also examined the effects of overexpression of wild-type (WT), T286D phosphomimic, T253D phosphomimic and T253V phosphonull forms of CaMKIIa in MDA-MB-231 breast cancer and SHSY5Y neuroblastoma cells on cellular proliferation and cell cycle progression. We demonstrate herein that whilst there is no change in total CaMKII expression or T286 phosphorylation throughout the cell cycle, a marked dephosphorylation of CaMKII at T253 occurs during the G2 and/or M phases. Additionally, we show by molecular inhibition, as well as pharmacological activation, that protein phosphatase 2A (PP2A) is the phosphatase responsible for this dephosphorylation. Furthermore, we show that inducible overexpression of WT, T286D and T253V forms of CaMKIIa in MDA-MB-231 and SHSY5Y cells increases cellular proliferation, with no alteration in cell cycle profiles. By contrast, overexpression of a T253D phosphomimic form of CaMKIIa significantly decreases proliferation, and cells accumulate in mitosis, specifically in metaphase. Taken together, these results strongly suggest that the dephosphorylation of CaMKII at T253 is involved in controlling the cell cycle, specifically the metaphase-anaphase transition. © 2014 Elsevier Inc.

DOI 10.1016/j.cellsig.2013.12.015
Citations Scopus - 4Web of Science - 4
Co-authors Nikki Verrills, Phil Dickson, Kathryn Skelding
2014 Abdul Majeed ABB, Pearsall E, Carpenter H, Brzozowski J, Dickson PW, Rostas JAP, Skelding KA, 'CaMKII Kinase Activity, Targeting and Control of Cellular Functions: Effect of Single and Double Phosphorylation of CaMKIIa', Calcium Signaling, 1 36-51 (2014) [C1]
Co-authors Phil Dickson, Kathryn Skelding
2013 Skelding KA, Majeed ABA, Carpenter H, Dickson PW, Rostas JA, 'Can functional outcomes of CaMKII double phosphorylation be predicted from outcomes following single phosphorylation of CaMKII?', JOURNAL OF NEUROCHEMISTRY, 125 162-162 (2013) [E3]
Co-authors Kathryn Skelding, Phil Dickson
2012 Skelding KA, Dickson PW, Verrills NM, Rostas JA, 'Progression through mitosis can be controlled by dephosphorylation of CaMKII at T253', JOURNAL OF NEUROCHEMISTRY, 123 31-31 (2012) [E3]
Co-authors Kathryn Skelding, Nikki Verrills, Phil Dickson
2012 Rostas JA, Skelding KA, Fluechter L, Dickson PW, Spratt NJ, 'CaMKII is Differentially Regulated in Striatum and Cortex', JOURNAL OF NEUROCHEMISTRY, 123 63-63 (2012) [E3]
Co-authors Phil Dickson, Neil Spratt, Kathryn Skelding
2012 Majeed ABA, Rostas JAP, Carpenter H, Dickson PW, Skelding KA, 'Multi-site phosphorylation of CaMKII regulates CaMKII function co-operatively', JOURNAL OF NEUROCHEMISTRY, 123 116-116 (2012) [E3]
Co-authors Kathryn Skelding, Phil Dickson
2012 Skelding KA, Spratt NJ, Fluechter L, Dickson PW, Rostas JA, 'alpha CaMKII is differentially regulated in brain regions that exhibit differing sensitivities to ischemia and excitotoxicity', Journal of Cerebral Blood Flow and Metabolism, 32 2181-2192 (2012) [C1]
Citations Scopus - 8Web of Science - 8
Co-authors Kathryn Skelding, Neil Spratt, Phil Dickson
2011 Skelding KA, Rostas JA, Verrills NM, 'Controlling the cell cycle: The role of calcium/calmodulin-stimulated protein kinases I and II', Cell Cycle, 10 631-639 (2011) [C1]
DOI 10.4161/cc.10.4.14798
Citations Scopus - 36Web of Science - 37
Co-authors Kathryn Skelding, Nikki Verrills
2010 Atkinson RJ, Rostas JA, Hunter M, 'Changes in mid-to-late latency auditory evoked responses in the chicken during neural maturation', Developmental Psychobiology, 52 24-34 (2010) [C1]
DOI 10.1002/dev.20408
Co-authors Mick Hunter
2010 Skelding KA, Suzuki T, Gordon SL, Xue J, Verrills NM, Dickson PW, Rostas JA, 'Regulation of CaMKII by phospho-Thr253 or phospho-Thr286 sensitive targeting alters cellular function', Cellular Signalling, 22 759-769 (2010) [C1]
DOI 10.1016/j.cellsig.2009.12.011
Citations Scopus - 11Web of Science - 11
Co-authors Nikki Verrills, Kathryn Skelding, Phil Dickson
2009 Skelding KA, Liao X, Verrills NM, Fluechter L, Dickson PW, Rostas JA, 'CaMKII phosphorylation at T253 alters neuronal growth rates and morphology', Journal of Neurochemistry, 110, Suppl. 2 42 (2009) [E3]
DOI 10.1111/j.1471-4159.2009.06238.x
Co-authors Phil Dickson, Nikki Verrills, Kathryn Skelding
2009 Rostas JA, Skelding KA, Verrills NM, Suzuki PW, Dickson T, 'CaMKII binding partners vary with cell type and phosphorylation state', Journal of Neurochemistry, 110, Suppl. 2 40-41 (2009) [E3]
DOI 10.1111/j.1471-4159.2009.06238.x
Co-authors Nikki Verrills, Kathryn Skelding
2009 Rosso OA, Mendes ADS, Rostas JA, Hunter M, Moscato PA, 'Distinguishing childhood absence epilepsy patients from controls by the analysis of their background brain electrical activity', Journal of Neuroscience Methods, 177 461-468 (2009) [C1]
DOI 10.1016/j.jneumeth.2008.10.017
Citations Scopus - 9Web of Science - 9
Co-authors Pablo Moscato, Mick Hunter, Alexandre Mendes
2009 Rosso OA, Mendes ADS, Berretta RE, Rostas JA, Hunter M, Moscato PA, 'Distinguishing childhood absence epilepsy patients from controls by the analysis of their background brain electrical activity (II): A combinatorial optimization approach for electrode selection', Journal of Neuroscience Methods, 181 257-267 (2009) [C1]
DOI 10.1016/j.jneumeth.2009.04.028
Citations Scopus - 13Web of Science - 14
Co-authors Mick Hunter, Regina Berretta, Pablo Moscato, Alexandre Mendes
2009 Skelding KA, Rostas JA, 'Regulation of CaMKII in vivo: The importance of targeting and the intracellular microenvironment', Neurochemical Research, 34 1792-1804 (2009) [C1]
DOI 10.1007/s11064-009-9985-9
Citations Scopus - 24Web of Science - 25
Co-authors Kathryn Skelding
2008 Gurd JW, Rawof S, Zhen Huo J, Dykstra C, Bissoon N, Teves L, et al., 'Ischemia and status epilepitcus result in enhanced phosphorylation of calcium and calmodulin-stimulated protein kinase II on threonine 253', Brain Research, 1218 158-165 (2008) [C1]
DOI 10.1016/j.brainres.2008.04.040
Citations Scopus - 7Web of Science - 7
2008 Atkinson RJ, Migues PV, Hunter M, Rostas JA, 'Molecular changes in the intermediate medial mesopallium after a one trial avoidance learning in immature and mature chickens', Journal of Neurochemistry, 104 891-902 (2008) [C1]
DOI 10.1111/j.1471-4159.2007.05060.x
Citations Scopus - 4Web of Science - 4
Co-authors Mick Hunter
2008 Skelding KA, Verrills NM, Fluechter L, Sim AT, Dickson PW, Rostas JA, 'Development of a novel method for the identification of CaMKII binding proteins', Journal of Neurochemistry, 106 51 (2008) [E3]
Co-authors Phil Dickson, Alistair Sim, Nikki Verrills, Kathryn Skelding
2008 Atkinson RJ, Migues PV, Cammarota M, Kavanagh JM, Hunter M, Rostas JA, 'Biochemical, behavioural and electrophysiological investigations of brain maturation in chickens', Brain Research Bulletin, 76 217-223 (2008) [C1]
DOI 10.1016/j.brainresbull.2008.02.036
Citations Scopus - 5Web of Science - 4
Co-authors Mick Hunter
2008 Gibbs ME, Gibbs C, Csillag A, Matsushima T, Rogers L, Rostas JA, 'Brain mechanisms, cognition and behaviour in birds', Brain Research Bulletin, 76 167-169 (2008) [C3]
DOI 10.1016/j.brainresbull.2008.03.012
Citations Scopus - 5Web of Science - 4
2007 Migues PV, Cammarota MP, Kavanagh J, Atkinson RJ, Powis DA, Rostas JA, 'Maturational changes in the subunit composition of AMPA receptors and the functional consequences of their activation in chicken forebrain', Developmental Neuroscience, 29 232-240 (2007) [C1]
DOI 10.1159/000096408
Citations Scopus - 14Web of Science - 14
2006 Sim AT, Herd LM, Proctor DT, Baldwin ML, Meunier FA, Rostas JA, 'High throughput analysis of endogenous glutamate release using a fluorescence plate reader', Journal of Neuroscience Methods, 153 43-47 (2006) [C1]
DOI 10.1016/j.jneumeth.2005.10.004
Citations Scopus - 5Web of Science - 6
Co-authors Lynn Herd, Alistair Sim
2006 Baldwin ML, Cammarota MP, Sim AT, Rostas JA, 'Src family tyrosine kinases differentially modulate exocytosis from rat brain nerve terminals', Neurochemistry International, 49 80-86 (2006) [C1]
DOI 10.1016/j.neuint.2006.01.002
Citations Scopus - 12Web of Science - 11
Co-authors Alistair Sim
2006 Migues PV, Lehmann IT, Fluechter L, Cammarota MP, Gurd JW, Sim AT, et al., 'Phosphorylation of CaMKII at Thr253 occurs in vivo and enhances binding to isolated postsynaptic densities', Journal of Neurochemistry, 98 289-299 (2006) [C1]
DOI 10.1111/j.1471-4159.2006.03876.x
Citations Scopus - 25Web of Science - 23
Co-authors Phil Dickson, Alistair Sim
2005 Rosso OA, Hyslop WA, Gerlach RH, Smith RL, Rostas JA, Hunter M, 'Quantitative EEG analysis of the maturational changes associated with childhood absence epilepsy', Physica A -Statistical Mechanics and Its Applications, 356 184-189 (2005) [C1]
DOI 10.1016/j.physa.2005.05.034
Citations Scopus - 13Web of Science - 13
Co-authors Mick Hunter
2005 Hunter M, Smith RLL, Hyslop W, Rosso OA, Gerlach R, Rostas JAP, et al., 'The Australian EEG database', CLINICAL EEG AND NEUROSCIENCE, 36 76-81 (2005) [C2]
Citations Scopus - 25Web of Science - 19
Co-authors Frans Henskens, Mick Hunter
2004 Xue J, Tsang C, Gai W-P, Malladi C, Trimble W, Rostas JA, Robinson P, 'Septin 3 (G-septin) is a developmentally regulated phosphoprotein enriched in presynaptic nerve terminals', Journal of Neurochemistry, 91(3) 579-590 (2004) [C1]
DOI 10.1111/j.1471-4159.2004.02755.x
Citations Scopus - 53Web of Science - 49
2004 Xue J, Milburn P, Hanna B, Graham M, Rostas JA, Robinson P, 'Phosphorylation of septin 3 on Ser-91 by cGMP-dependent protein kinase-I in nerve terminals', Biochem J, 381 753-760 (2004) [C1]
DOI 10.1042/BJ20040455
Citations Scopus - 23Web of Science - 16
2004 Lengyel I, Voss K, Cammarota MP, Bradshaw K, Brent VA, Murphy KPSJ, et al., 'Autonomous activity of CaMKII is only transiently increased following the induction of long-term potentiation in the rat hippocampus', European Journal of Neuroscience, 20 3063-3072 (2004) [C1]
DOI 10.1111/j.1460-9568.2004.03748.x
Citations Scopus - 61Web of Science - 59
2003 Hunter M, Rostas JA, Hyslop WA, 'The John Hunter Hospital EEG database project', Australian Journal of Psychology, 55 104 (2003) [C3]
Co-authors Mick Hunter
2003 Baldwin ML, Rostas JA, Sim AT, 'Two modes of excytosis from synaptosomes are differentially regulated by protein phosphatase types 2A and 2B', Journal of Neurochemistry, 85 1190-1199 (2003) [C1]
DOI 10.1046/j.1471-4159.2003.01779.x
Citations Scopus - 27Web of Science - 26
Co-authors Alistair Sim
2003 Cammarota MP, Bevilaqua L, Rostas JA, Dunkley PR, 'Histamine activates tyrosine hydroxylase in bovine adrenal chromaffin cells through a pathway that involves ERK1/2 but not p38 or JNK', Journal of Neurochemistry, 84 453-458 (2003) [C1]
DOI 10.1046/j.1471-4159.2003.01517.x
Citations Scopus - 25Web of Science - 25
Co-authors Peter Dunkley
2003 Sim AT, Baldwin ML, Rostas JA, Holst J, Ludowyke RI, 'The role of serine/threonine protein phosphatases in exocytosis', Biochemical Journal, 373 641-659 (2003) [C1]
DOI 10.1042/BJ20030484
Citations Scopus - 37Web of Science - 36
Co-authors Alistair Sim
2003 Atkinson RJ, Bevilaqua L, Rostas JA, Hunter M, 'Discriminative taste aversion learning: A learning task for older chickens', Neurobiology of Learning and Memory, 79 25-31 (2003)
DOI 10.1016/S1074-7427(02)00011-4
Citations Scopus - 5Web of Science - 5
Co-authors Mick Hunter
2001 Lengyel I, Voss K, Brent V, Cammarota M, Murphy K, Rostas JAP, Bliss TVP, 'Activation and autophosphorylation of CaMPK-II under conditions that do or do not induce LTP', JOURNAL OF NEUROCHEMISTRY, 78 13-13 (2001)
2001 Lengyel I, Cammarota MP, Brent VA, Rostas JA, 'Autonomous activity and autophosphorylation of CAMPK-II in rat hippocampal slices: effects of tissue preparation', Journal of Neurochemistry, 76 149-154 (2001) [C1]
Citations Scopus - 9Web of Science - 9
2001 Cammarota MP, Bevilaqua L, Dunkley PR, Rostas JA, 'Angiotensin II Promotes the Phosphorylation of Cyclic AMP-Responsive Element Binding Protein (CREB) at Ser133 Through an ERK1/2-Dependent Mechanism', Journal of Neurochemistry, 79 1122-1128 (2001) [C1]
Citations Scopus - 33Web of Science - 31
Co-authors Peter Dunkley
2001 Lengyel I, Nairn AC, McCluskey A, Toth G, Penke B, Rostas JAP, 'Auto-inhibition of Ca2+/calmodulin-dependent protein kinase II by its ATP-binding domain', Journal of Neurochemistry, 76 1066-1072 (2001) [C1]
Citations Scopus - 11Web of Science - 9
Co-authors Adam Mccluskey
2001 Cammarota MP, Bevilaqua L, Dunkley PR, Rostas JA, 'Angiotensin II Promotes a Rapid and Reversible Increase in SRC-Tyrosine Kinase Activity in Bovine Adrenal Chromaffin Cells: Effect on MAPK Pathways', Proceedings of the Australian Neuroscience Society, 12 197 (2001) [C3]
Co-authors Peter Dunkley
2001 Cammarota MP, Bevilaqua L, Dunkley PR, Rostas JA, 'Angiotensin II Promotes a Src and ERK 1/2 Dependent Increase in SER133-CREB Phosphorylation', Journal of Neurochemistry, 78 Supl. 1 143 (2001) [C3]
Co-authors Peter Dunkley
2000 Hunter M, Battilana M, Bragg T, Rostas J, 'EEG as a measure of developmental changes in the chicken brain', Developmental Psychobiology, 36 23-28 (2000) [C1]
Citations Scopus - 14Web of Science - 12
Co-authors Mick Hunter
2000 Lengyel I, Fieuw-Makaroff S, Hall AL, Sim AT, Rostas JA, Dunkley PR, 'Modulation of the Phosphorylation and Activity of Calcium/Calmodulin-Dependent Protein Kinase II by Zinc', Journal of Neurochemistry, 75 No. 2 594-605 (2000) [C1]
Citations Scopus - 57Web of Science - 55
Co-authors Alistair Sim, Peter Dunkley
2000 Margrie TW, Rostas JA, Sah P, 'Inhibition of transmitter release and long-term depression in the avian hippocampus', Neuroscience Letters, 284 17-20 (2000) [C1]
Citations Scopus - 4Web of Science - 3
2000 Xue J, Wang X, Malladi CS, Kinoshita M, Milburn PJ, Lengyel I, et al., 'Phosphorylatin of a new brain-specific septin, G-Septin, by cyclic-GMP dependent protein kinase', Biological Chemistry, 275 10047-10056 (2000) [C1]
Citations Scopus - 48Web of Science - 42
1999 Lengyel I, Hall AL, Fieuw-Makaroff S, Sim ATR, Rostas JAP, Dunkley PR, 'Characterization of the zinc-dependent autophosphorylation sites on calcium/calmodulin-dependent protein kinase II', JOURNAL OF NEUROCHEMISTRY, 73 S33-S33 (1999)
Co-authors Alistair Sim, Peter Dunkley
1999 Lengyel I, Brent V, Rostas JAP, 'Autonomous CaMPK-II activity in rat hippocapal slices: Effects of tissue handling', JOURNAL OF NEUROCHEMISTRY, 73 S36-S36 (1999)
1999 Rostas JAP, Sievert AD, McCoy K, Lengyel I, 'Mossy fibre-like synapses in chicken hippocampus', JOURNAL OF NEUROCHEMISTRY, 73 S40-S40 (1999)
1999 Lengyel I, Olson LG, Nichol K, Brain K, Wang X, Robinson PJ, et al., 'Phosphorylation of proteins in chick ciliary ganglion under conditions that induce long-lasting changes in synaptic transmission: phosphoprotein targets for nitric oxide action', Neuroscience, 90 No. 2 607-619 (1999) [C1]
Citations Scopus - 5Web of Science - 3
1998 Rostas JAP, 'Nitric oxide responsive protein phosphorylation in neuronal tissue', JOURNAL OF NEUROCHEMISTRY, 70 S4-S4 (1998)
1998 Capon RJ, Rooney F, Murray LM, Collins E, Sim AT, Rostas JA, et al., 'Dragmacidins: New protein phosphatase inhibitors from a Southern Australian deep-water marine sponge Spongosorites sp', Journal of Natural Products, 61 660-662 (1998) [C1]
Citations Scopus - 120Web of Science - 111
Co-authors Alistair Sim
1998 Wang X, Rostas JA, 'Protein phosphorylation in fast and slow chicken skeletal muscles: Effect of denervation', Muscle and Nerve, 21 504-513 (1998) [C1]
Citations Scopus - 2Web of Science - 2
1998 Margrie TW, Rostas JA, Sah P, 'Presynaptic long-term depression at a central glutamatergic synapse: a role for CaMKII', Nature Neuroscience, 1(5) 378-383 (1998) [C1]
Citations Scopus - 13Web of Science - 26
1998 Lengyel I, Nichol KA, Bennett MR, Heath JW, Little GJ, Rostas JA, 'Alpha and beta subunits of CaM-kinase II are localized in different neurons in chick ciliary ganglion', NeuroReport, 9(12) 2753-2755 (1998) [C1]
Citations Scopus - 2Web of Science - 2
1998 Sim AT, Collins E, Mudge LM, Rostas JA, 'Developmental regulation of protein phosphatase types 1 and 2A in post-hatch chicken brain', Neurochemical Research, 23(4) 487-491 (1998) [C1]
Citations Scopus - 12Web of Science - 11
Co-authors Alistair Sim
1998 Margrie TW, Rostas JA, Sah P, 'Long-term potentiation of synaptic transmission in the avian hippocampus', The Journal of Neuroscience, 18(4) 1207-1216 (1998) [C1]
Citations Scopus - 42Web of Science - 40
1997 Sansom AJ, Brent VA, Jarvie PE, Darlington CL, Smith PF, Laverty R, Rostas JAP, 'In vitro phosphorylation of medial vestibular nucleus and prepositus hypoglossi proteins during behavioural recovery from unilateral vestibular deafferentation in the guinea pig', BRAIN RESEARCH, 778 166-177 (1997)
DOI 10.1016/S0006-8993(97)01059-7
Citations Scopus - 18Web of Science - 17
1997 Margrie TW, Rostas JAP, 'Subcellular translocation of Ca2+(calmodulin-dependent protein kinase II: Fact or artifact?', JOURNAL OF NEUROCHEMISTRY, 69 435-436 (1997)
Citations Web of Science - 2
1997 Margrie TW, Rostas JAP, Sah P, 'NMDA receptor independent long-term potentiation in the avian hippocampus', JOURNAL OF NEUROCHEMISTRY, 69 S66-S66 (1997)
1997 Lengyel I, Olesen L, Wang X, Nichol KA, Bennett MR, Rostas PJ, Rostas JAP, 'Nitric oxide responsive phosphoproteins (NORPs) in chicken ciliary ganglion', JOURNAL OF NEUROCHEMISTRY, 69 S187-S187 (1997)
1997 Sansom AJ, Brent VA, Jarvie PE, Darlington CL, Smith CL, Laverty R, Rostas JAP, 'In vitro phosphorylation of medial vestibular nucleus and prepositus hypoglossi proteins during behavioural recovery from unilateral vestibular deafferentation in the guinea pig', JOURNAL OF NEUROCHEMISTRY, 69 S208-S208 (1997)
1997 Margrie TW, Rostas JAP, Suzuki T, 'Subcellular translocation of Ca2+/calmodulin-dependent protein kinase II: Fact or artifact? [1] (multiple letters)', Journal of Neurochemistry, 69 435-437 (1997)
Citations Scopus - 4
1996 Wang X, Rostas JAP, 'Effect of hypothyroidism on the subcellular distribution of Ca2+ calmodulin-stimulated protein kinase II in chicken brain during posthatch development', JOURNAL OF NEUROCHEMISTRY, 66 1625-1632 (1996)
Citations Scopus - 7Web of Science - 8
1996 Rostas JAP, Brent VA, Voss K, Errington ML, Bliss TVP, Gurd JW, 'Enhanced tyrosine phosphorylation of the 2B subunit of the N-methyl-D-aspartate receptor in long-term potentiation', PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 93 10452-10456 (1996)
DOI 10.1073/pnas.93.19.10452
Citations Scopus - 194Web of Science - 196
1996 Lengyel I, Nichol KA, Sim ATR, Bennett MR, Dunkley PR, Rostas JAP, 'Characterization of protein kinase and phosphatase systems in chick ciliary ganglion', NEUROSCIENCE, 70 577-588 (1996)
DOI 10.1016/0306-4522(95)00356-8
Citations Scopus - 8Web of Science - 9
Co-authors Peter Dunkley, Alistair Sim
1995 KAVANAGH JM, BUNN SJ, BOYD TL, ROSTAS JAP, 'DEVELOPMENTAL-CHANGES IN GLUTAMATE-RECEPTOR STIMULATED INOSITOL PHOSPHOLIPID-METABOLISM AND CA-45(2+)-ACCUMULATION IN POSTHATCH CHICKEN FOREBRAIN', NEUROSCIENCE LETTERS, 194 161-164 (1995)
DOI 10.1016/0304-3940(95)11749-M
Citations Scopus - 8Web of Science - 8
1995 HEATH JW, LITTLE GJ, ROBINSON CS, SCHULMAN H, ROSTAS JAP, 'LM AND EM IMMUNOLOCALIZATION OF ALPHA-CAMPK-II AND BETA-CAMPK-II', JOURNAL OF NEUROCHEMISTRY, 65 S22-S22 (1995)
1995 SIM ATR, RATCLIFFE E, ROSTAS JAP, 'DIFFERENTIAL REGULATION OF MEMBRANE-BOUND PROTEIN PHOSPHATASES IN BRAIN', JOURNAL OF NEUROCHEMISTRY, 65 S93-S93 (1995)
Co-authors Alistair Sim
1994 SIM ATR, RATCLIFFE E, MUMBY MC, VILLAMORUZZI E, ROSTAS JAP, 'DIFFERENTIAL ACTIVITIES OF PROTEIN PHOSPHATASE TYPE-1 AND TYPE-2A IN CYTOSOLIC AND PARTICULATE FRACTIONS FROM RAT FOREBRAIN', JOURNAL OF NEUROCHEMISTRY, 62 1552-1559 (1994) [C1]
Citations Scopus - 47Web of Science - 50
Co-authors Alistair Sim
1994 SIM ATR, RATCLIFFE E, ROSTAS JAP, 'SELECTIVE REGULATION OF PROTEIN PHOSPHATASE-I DURING DEVELOPMENT IN CHICKEN FOREBRAIN AND CEREBELLUM', JOURNAL OF NEUROCHEMISTRY, 63 S73-S73 (1994)
Co-authors Alistair Sim
1994 MARTIN H, ROSTAS J, PATEL Y, AITKEN A, 'SUBCELLULAR-LOCALIZATION OF 14-3-3-ISOFORMS IN RAT-BRAIN USING SPECIFIC ANTIBODIES', JOURNAL OF NEUROCHEMISTRY, 63 2259-2265 (1994)
Citations Scopus - 126Web of Science - 122
1993 MURRAY L, SIM ATR, ROSTAS JAP, CAPON RJ, 'ISOPALINURIN - A MILD PROTEIN PHOSPHATASE INHIBITOR FROM A SOUTHERN AUSTRALIAN MARINE SPONGE, DYSIDEA SP', AUSTRALIAN JOURNAL OF CHEMISTRY, 46 1291-1294 (1993)
Citations Scopus - 10Web of Science - 9
Co-authors Alistair Sim
1993 MURRAY L, SIM ATR, ROSTAS JAP, CAPON RJ, 'ISOPALINURIN - A MILD PROTEIN PHOSPHATASE INHIBITOR FROM A SOUTHERN AUSTRALIAN MARINE SPONGE, DYSIDEA SP (VOL 46, PG 1291-1294, 1993)', AUSTRALIAN JOURNAL OF CHEMISTRY, 46 1824-1824 (1993)
Co-authors Alistair Sim
1993 CAPON RJ, ELSBURY K, BUTLER MS, LU CC, HOOPER JNA, ROSTAS JAP, et al., 'EXTRAORDINARY LEVELS OF CADMIUM AND ZINC IN A MARINE SPONGE, TEDANIA-CHARCOTI TOPSENT - INORGANIC CHEMICAL DEFENSE AGENTS', EXPERIENTIA, 49 263-264 (1993)
DOI 10.1007/BF01923536
Citations Scopus - 24Web of Science - 24
Co-authors Alistair Sim
1993 SHAHIN S, BARTLETT PF, MILLAR TJ, MCLENNAN I, ROSTAS JAP, 'DISTRIBUTION OF THE SLOW CARDIAC ISOFORM OF SKELETAL-MUSCLE CA2+-ATPASE IN DEVELOPING AND MATURE TISSUES OF CHICKENS DETERMINED USING A MONOCLONAL-ANTIBODY', JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 41 215-224 (1993)
1993 KAVANAGH JM, BOYD TL, BUNN SJ, ROSTAS JAP, 'QUISQUALATE RECEPTOR FUNCTION IN CHICKEN FOREBRAIN', JOURNAL OF NEUROCHEMISTRY, 61 S16-S16 (1993)
1993 SIM ATR, RATCLIFFE E, DUNKLEY PR, ROSTAS JAP, 'MEMBRANE-BOUND FORMS OF PROTEIN PHOSPHATASE TYPE-1 AND TYPE-2A HAVE LOWER ACTIVITY THAN CYTOSOLIC FORMS IN BRAIN', JOURNAL OF NEUROCHEMISTRY, 61 S136-S136 (1993)
Co-authors Alistair Sim, Peter Dunkley
1993 WANG X, ROSTAS JAP, 'MATURATION CHANGES IN CAM-PKII IN CHICKEN CEREBELLUM', JOURNAL OF NEUROCHEMISTRY, 61 S219-S219 (1993)
1993 LENGYEL I, NICHOL KA, BENNETT MR, ROSTAS JAP, DUNKLEY PR, 'PROTEIN-PHOSPHORYLATION IN CHICK CILIARY GANGLION', JOURNAL OF NEUROCHEMISTRY, 61 S221-S221 (1993)
Co-authors Peter Dunkley
1993 KAVANAGH JM, ROSTAS JAP, 'AMPA RECEPTOR FUNCTION IN CHICKEN FOREBRAIN', JOURNAL OF NEUROCHEMISTRY, 61 S269-S269 (1993)
1993 SIM ATR, LLOYD HGE, JARVIE PE, MORRISON M, ROSTAS JAP, DUNKLEY PR, 'SYNAPTOSOMAL AMINO-ACID RELEASE - EFFECT OF INHIBITING PROTEIN PHOSPHATASES WITH OKADAIC ACID', NEUROSCIENCE LETTERS, 160 181-184 (1993)
DOI 10.1016/0304-3940(93)90408-D
Citations Scopus - 18Web of Science - 19
Co-authors Peter Dunkley, Alistair Sim
1992 ROSTAS JAP, DUNKLEY PR, 'MULTIPLE FORMS AND DISTRIBUTION OF CALCIUM/CALMODULIN-STIMULATED PROTEIN KINASE-II IN BRAIN', JOURNAL OF NEUROCHEMISTRY, 59 1191-1202 (1992)
DOI 10.1111/j.1471-4159.1992.tb08428.x
Citations Scopus - 40Web of Science - 49
Co-authors Peter Dunkley
1992 HEATH JW, GLENFIELD PJ, ROSTAS JAP, 'STRUCTURAL MATURATION OF SYNAPSES IN THE RAT SUPERIOR CERVICAL-GANGLION CONTINUES BEYOND 4 WEEKS OF AGE', NEUROSCIENCE LETTERS, 142 17-21 (1992)
DOI 10.1016/0304-3940(92)90610-J
Citations Scopus - 2Web of Science - 3
1992 KAVANAGH JM, POWIS DA, DODD PR, ROSTAS JAP, 'NMDA RECEPTOR FUNCTION IN CHICKEN FOREBRAIN DURING MATURATION', MOLECULAR NEUROPHARMACOLOGY, 2 193-195 (1992)
1991 WEINBERGER RP, ROSTAS JAP, 'EFFECT OF ZINC ON CALMODULIN-STIMULATED PROTEIN KINASE-II AND PROTEIN-PHOSPHORYLATION IN RAT CEREBRAL-CORTEX', JOURNAL OF NEUROCHEMISTRY, 57 605-614 (1991)
DOI 10.1111/j.1471-4159.1991.tb03791.x
Citations Scopus - 21Web of Science - 25
1991 SIM ATR, DUNKLEY PR, JARVIE PE, ROSTAS JAP, 'MODULATION OF SYNAPTOSOMAL PROTEIN-PHOSPHORYLATION DEPHOSPHORYLATION BY CALCIUM IS ANTAGONIZED BY INHIBITION OF PROTEIN PHOSPHATASES WITH OKADAIC ACID', NEUROSCIENCE LETTERS, 126 203-206 (1991)
DOI 10.1016/0304-3940(91)90554-7
Citations Scopus - 27Web of Science - 29
Co-authors Peter Dunkley, Alistair Sim
1991 KAVANAGH JM, DODD PR, ROSTAS JAP, '[H-3] MK-801 BINDING IN IMMATURE AND MATURE CHICKEN FOREBRAIN', NEUROSCIENCE LETTERS, 134 83-87 (1991)
DOI 10.1016/0304-3940(91)90514-T
Citations Web of Science - 13
1991 Kavanagh JM, Dodd PR, Rostas JAP, '[3H]MK-801 binding in immature and mature chicken forebrain', Neuroscience Letters, 134 83-87 (1991)

Previous studies have shown that the rate of calcium uptake stimulated by N-methyl-d-aspartate (NMDA) increased during the maturation phase of synapse development in chicken foreb... [more]

Previous studies have shown that the rate of calcium uptake stimulated by N-methyl-d-aspartate (NMDA) increased during the maturation phase of synapse development in chicken forebrain. To investigate whether this change in function is due to a change in the properties of NMDA receptor associated ion channels, we measured the binding of [3H]MK-801 (a ligand which binds to the NMDA receptor associated ion channel) to membranes from immature and mature chicken brain. The binding properties of MK-801 in chicken brain were similar to those in mammalian brain. There was no significant difference in any of the binding parameters measured at the two ages, i.e. KD, Bmax and optimal glutamate concentration for and maximal enhancement by glutamate of MK-801 binding. These results suggest that there is no change in the NMDA operated ion-channels during maturation. Thus the maturational change in NMDA receptor function could be due to: a change in the agonist portion of the NMDA receptor, a change in the regulation of the receptor/ionophore complex, perhaps by the postsynaptic density whose structure and composition changes during the same period, or a change in the number of voltage-sensitive calcium channels recruited as a result of NMDA receptor activation. © 1991.

DOI 10.1016/0304-3940(91)90514-T
Citations Scopus - 12
1991 JEITNER TM, JARVIE PE, COSTA M, ROSTAS JAP, DUNKLEY PR, 'PROTEIN-PHOSPHORYLATION IN GUINEA-PIG MYENTERIC GANGLIA AND BRAIN - PRESENCE OF CALMODULIN KINASE-II, PROTEIN-KINASE-C AND CYCLIC-AMP KINASE AND CHARACTERIZATION OF MAJOR PHOSPHOPROTEINS', NEUROSCIENCE, 40 555-569 (1991)
DOI 10.1016/0306-4522(91)90141-A
Citations Scopus - 11Web of Science - 14
Co-authors Peter Dunkley
1991 KOSZKA C, BRENT VA, ROSTAS JAP, 'DEVELOPMENTAL-CHANGES IN PHOSPHORYLATION OF MAP-2 AND SYNAPSIN-I IN CYTOSOL AND TAXOL POLYMERIZED MICROTUBULES FROM CHICKEN BRAIN', NEUROCHEMICAL RESEARCH, 16 637-644 (1991)
DOI 10.1007/BF00965549
Citations Scopus - 13Web of Science - 9
1991 ROSTAS JAP, KAVANAGH JM, DODD PR, HEATH JW, POWIS DA, 'MECHANISMS OF SYNAPTIC PLASTICITY - CHANGES IN POSTSYNAPTIC DENSITIES AND GLUTAMATE RECEPTORS IN CHICKEN FOREBRAIN DURING MATURATION', MOLECULAR NEUROBIOLOGY, 5 203-216 (1991)
DOI 10.1007/BF02935546
Citations Scopus - 26Web of Science - 22
1990 SHAHIN S, JEFFREY PL, ROSTAS JAP, 'THY-1-LIKE IMMUNOREACTIVITY IN DEVELOPING CHICKEN SKELETAL-MUSCLE - IDENTIFICATION OF A CROSS-REACTIVE SLOW-FIBER SPECIFIC MOLECULE THAT IS NOT THY-1', JOURNAL OF NEUROSCIENCE RESEARCH, 26 45-54 (1990)
DOI 10.1002/jnr.490260106
Citations Scopus - 1Web of Science - 3
1990 Wang X, Rostas JA, 'Development of Ca2(+)-calmodulin activated protein kinase II and cAMP activated protein kinase in the central nervous system', Sheng li ke xue jin zhan [Progress in physiology], 21 58-60 (1990)
1990 WEBB GR, REDMAN S, HENNRIKUS D, ROSTAS JAP, SANSONFISHER RW, 'THE PREVALENCE AND SOCIODEMOGRAPHIC CORRELATES OF HIGH-RISK AND PROBLEM DRINKING AT AN INDUSTRIAL WORKSITE', BRITISH JOURNAL OF ADDICTION, 85 495-507 (1990)
Citations Scopus - 27Web of Science - 21
Co-authors Rob Sanson-Fisher
1989 SHAHIN S, LASHER RS, MILLAR TJ, ROSTAS JAP, 'A MONOCLONAL-ANTIBODY AGAINST THE SLOW ISOFORM OF SKELETAL-MUSCLE CA-2+-ATPASE SELECTIVELY STAINS A SUBPOPULATION OF NEURONS IN THE CENTRAL NERVOUS-SYSTEM', NEUROSCIENCE LETTERS, 106 163-168 (1989)
DOI 10.1016/0304-3940(89)90220-6
Citations Scopus - 2Web of Science - 3
1989 ROSTAS JAP, BRENT VA, SECCOMBE M, WEINBERGER RP, DUNKLEY PR, 'PURIFICATION AND CHARACTERIZATION OF CALMODULIN-STIMULATED PROTEIN KINASE-II FROM 2-DAY AND ADULT CHICKEN FOREBRAIN', JOURNAL OF MOLECULAR NEUROSCIENCE, 1 93-104 (1989)
DOI 10.1007/BF02918895
Citations Scopus - 9Web of Science - 10
Co-authors Peter Dunkley
1989 Rostas JAP, Brent VA, Seccombe M, Weinberger RP, Dunkley PR, 'Purification and characterization of calmodulin-stimulated protein kinase II from two-day and adult chicken forebrain', Journal of Molecular Neuroscience, 1 93-104 (1989)

Soluble calmodulin-stimulated protein kinase II has been purified from 2-day and adult chicken forebrain At both ages the holoenzyme eluted from a Superose-6B column with an appar... [more]

Soluble calmodulin-stimulated protein kinase II has been purified from 2-day and adult chicken forebrain At both ages the holoenzyme eluted from a Superose-6B column with an apparent molecular weight of approximately 700,000 daltons and contained three subunits. The subunits were found to be the counterparts of the alpha, beta, and betá subunits of the enzyme purified from adult rat brain in that they had one-dimensional phosphopeptide maps that were indistinguishable from those of the corresponding subunit in the rat enzyme and they migrated in SDS-polyacrylamide gels with the same apparent molecular weights. However, the doublet formed by the beta subunit was much more clearly resolved in the chicken enzyme and the betá subunit, which was much more abundant in the adult chicken than in the adult rat, was also found to be a doublet. The ratio of the concentrations of the alpha and beta subunits changed during development. By autoradiography following autophosphorylation, the alpha:beta ratios of the 2-day and adult enzymes were 0.89±0.07 and 1.92±0.26, reapectively; by silver staining the alpha:beta ratios were 0.95±0.11 and 1.85±0.17, respectively. The concentration of the betá subunit was equal to that of the beta subunit at both ages. Autophosphorylation produced a decrease in the electrophoretic mobility of the alpha and beta subunits in SDS-polyacrylamide gels and a marked decrease in the calcium dependence of the substrate phosphorylation activity of the enzyme at both ages. The purified enzyme from chicken brain appeared to be more stable under standard in vitro assay conditions than the rat enzyme, and this was particularly so for the enzyme from 2-day forebrain. © 1989 Humana Press Inc.

DOI 10.1007/BF02918895
Citations Scopus - 1
Co-authors Peter Dunkley
1988 DUNKLEY PR, HEATH JW, HARRISON SM, JARVIE PE, GLENFIELD PJ, ROSTAS JAP, 'A RAPID PERCOLL GRADIENT PROCEDURE FOR ISOLATION OF SYNAPTOSOMES DIRECTLY FROM AN S-1 FRACTION - HOMOGENEITY AND MORPHOLOGY OF SUBCELLULAR-FRACTIONS', BRAIN RESEARCH, 441 59-71 (1988)
DOI 10.1016/0006-8993(88)91383-2
Citations Scopus - 307Web of Science - 322
Co-authors Peter Dunkley
1988 Weinberger RP, Rostas JA, 'Developmental changes in protein phosphorylation in chicken forebrain. I. cAMP-stimulated phosphorylation.', Brain Research, 471 249-257 (1988)

The net level of cyclic AMP-stimulated protein phosphorylation was investigated in cytosolic and membrane fractions from chicken forebrain between embryonic day 13 (E13) and 52 da... [more]

The net level of cyclic AMP-stimulated protein phosphorylation was investigated in cytosolic and membrane fractions from chicken forebrain between embryonic day 13 (E13) and 52 days post-hatching. Throughout this period the majority of the net level of cAMP-stimulated phosphorylation of endogenous proteins was in the cytosolic fractions. Between day -8 (E13) and adult, the net level of cAMP-stimulated phosphorylation of endogenous proteins in the cytosol (S3) and crude synaptic plasma membrane (P2-M) fractions fell by 3 and 4 fold, respectively, when expressed per mg protein and rose by 5 and 10 fold, respectively, when expressed per fraction. The changes in specific activity were completed by 6-15 days post-hatching. The occluded cytosol (P2-S) fraction showed little change in the net level of cAMP-stimulated phosphorylation of endogenous proteins per mg protein. Major changes in phosphoprotein patterns involving both decreases and increases in phosphorylation occurred in all fractions from day -8 (E13) to day 6 post-hatch; thereafter the phosphoprotein bands and their relative intensities were unchanged. Three bands (P90 in S3; P41 and P31 in P2-M) contained major cAMP-stimulated phosphoproteins in embryonic brain but were absent after hatching. When cAMP-stimulated phosphorylation activity was measured in S3 and P-2M using an exogenous peptide substrate (Kemptide) there was no change in kinase activity per mg protein between day -8 (E13) and 30 days post-hatch. This suggests that the decrease in the net level of cAMP stimulated phosphorylation of endogenous proteins was due to the decrease in levels of endogenous phosphoproteins rather than protein kinase activity.(ABSTRACT TRUNCATED AT 250 WORDS)

Citations Scopus - 1
1988 Weinberger RP, Rostas JA, 'Developmental changes in protein phosphorylation in chicken forebrain. II. Calmodulin stimulated phosphorylation.', Brain Research, 471 259-272 (1988)

The development of calmodulin stimulated protein phosphorylation, with particular reference to calmodulin-stimulated protein kinase II (CMK II), was investigated in 3 subcellular ... [more]

The development of calmodulin stimulated protein phosphorylation, with particular reference to calmodulin-stimulated protein kinase II (CMK II), was investigated in 3 subcellular fractions of chicken forebrain: cytosol (S3), crude synaptic plasma membranes (P2-M) and occluded cytosol (P2-S). Changes in the level of calmodulin-stimulated phosphorylation of endogenous proteins occurred over a protracted time course and were not complete until after day 52 post-hatching. By day 15 post-hatching, calmodulin-stimulated phosphoproteins characteristic of embryonic fractions had all disappeared and those characteristic of adult tissue were present but not necessarily at their mature levels. The levels of CMK II were estimated from the autophosphorylation of the alpha-subunit which was the only phosphoprotein present at 53,000 Da in the 3 fractions. Overall, calmodulin-stimulated phosphorylation and CMK II levels were low in embryonic brain and high in adult brain but two specific changes in CMK II were observed during development: (1) although CMK II concentrations increased in both membrane and cytosolic fractions until day 23 the kinase was predominantly cytoplasmic (approximately 75%) until day 23, after which it became increasingly membrane bound so that by day 52 post-hatching the majority of CMK II was present in the synaptic membrane fraction, and (2) the relative concentrations of the alpha- and beta-subunits changed from an alpha:beta-value of approximately 1:1 in the 19 day embryo to approximately 1:2 by 15 days post-hatch after which no further change was seen. The occurrence of major changes in the calmodulin stimulated protein phosphorylation system for up to 6-8 weeks after synapse formation is completed in the forebrain, provides further support for the existence of a synapse maturation phase of neuronal differentiation which is distinct from synapse formation. This phase involves only a specific subset of the developmental changes occurring in the calmodulin-stimulated phosphorylation system.

Citations Scopus - 8
1988 ROSTAS JAP, SECCOMBE M, WEINBERGER RP, '2 DEVELOPMENTALLY REGULATED ISOENZYMES OF CALMODULIN-STIMULATED PROTEIN KINASE-II IN RAT FOREBRAIN', JOURNAL OF NEUROCHEMISTRY, 50 945-953 (1988)
DOI 10.1111/j.1471-4159.1988.tb03003.x
Citations Scopus - 40Web of Science - 48
1988 DUNKLEY PR, JARVIE PE, ROSTAS JAP, 'DISTRIBUTION OF CALMODULIN-STIMULATED AND CYCLIC AMP-STIMULATED PROTEIN-KINASES IN SYNAPTOSOMES', JOURNAL OF NEUROCHEMISTRY, 51 57-68 (1988)
DOI 10.1111/j.1471-4159.1988.tb04835.x
Citations Scopus - 14Web of Science - 20
Co-authors Peter Dunkley
1988 WEINBERGER RP, ROSTAS JAP, 'DEVELOPMENTAL-CHANGES IN PROTEIN-PHOSPHORYLATION IN CHICKEN FOREBRAIN .1. CAMP-STIMULATED PHOSPHORYLATION', DEVELOPMENTAL BRAIN RESEARCH, 43 249-257 (1988)
DOI 10.1016/0165-3806(88)90103-4
Citations Scopus - 10Web of Science - 11
1988 WEINBERGER RP, ROSTAS JAP, 'DEVELOPMENTAL-CHANGES IN PROTEIN-PHOSPHORYLATION IN CHICKEN FOREBRAIN .2. CALMODULIN STIMULATED PHOSPHORYLATION', DEVELOPMENTAL BRAIN RESEARCH, 43 259-272 (1988)
DOI 10.1016/0165-3806(88)90104-6
Citations Scopus - 12Web of Science - 19
1988 DICKINSON JA, ROSTAS JAP, NICHOLS B, EBELING W, DICKESON J, MATTHEWS D, et al., 'THE USE OF COTININE TO VALIDATE SMOKING SELF-REPORT IN PRIMARY CARE', COMMUNITY HEALTH STUDIES, 12 472-473 (1988)
1987 SHELDRAKE RF, CROCKER BD, HUSBAND AJ, ROSTAS JAP, 'ISOLATION AND PURIFICATION OF A THY-1-LIKE GLYCOPROTEIN FROM SHEEP BRAIN AND ITS DISTRIBUTION IN OVINE TISSUES', RESEARCH IN VETERINARY SCIENCE, 42 358-364 (1987)
Citations Scopus - 1Web of Science - 3
1987 Rostas JAP, Brent V, Dunkley PR, 'The effect of calmodulin and autophosphorylation on the activity of calmodulin-stimulated protein kinase II', Neuroscience Research Communications, 1 3-8 (1987)
Citations Scopus - 7
Co-authors Peter Dunkley
1986 DUNKLEY PR, JARVIE PE, HEATH JW, KIDD GJ, ROSTAS JAP, 'A RAPID METHOD FOR ISOLATION OF SYNAPTOSOMES ON PERCOLL GRADIENTS', BRAIN RESEARCH, 372 115-129 (1986)
DOI 10.1016/0006-8993(86)91464-2
Citations Scopus - 343Web of Science - 379
Co-authors Peter Dunkley
1986 Sedman GL, Jeffrey PL, Austin L, Rostas JA, 'The metabolic turnover of the major proteins of the postsynaptic density.', Brain Research, 387 221-230 (1986)

We have used the method of Austin, Lowry, Brown and Carter, to measure the steady-state metabolic half-life of tubulin (alpha and beta individually) and actin (beta and gamma toge... [more]

We have used the method of Austin, Lowry, Brown and Carter, to measure the steady-state metabolic half-life of tubulin (alpha and beta individually) and actin (beta and gamma together) in the total cytosolic (S3), microsomal (P3), synaptic plasma membrane (SPM) and synaptic junction (SJ) subcellular fractions from 6-day-old and adult chicken forebrain. In the SPM and SJ fractions we also measured the steady-state metabolic half-life of the major postsynaptic density protein (mPSDp). In SPM and SJ fractions from 6-day-old chickens tubulin and actin turned over approximately twice as slowly (t1/2 approximately equal to 24 days) as tubulin and actin in the S3 fraction (t1/2 approximately equal to 13 days). This difference was unlikely merely to be due to association with membranes since the t1/2 values for the proteins were the same in P3 and S3. The estimated t1/2 values for mPSDp were similar to that for tubulin and actin in SPM and SJ fractions. Similar results were obtained in adult chickens except that all t1/2 values in all fractions were approximately 30% larger. The calculated t1/2 values did not change between labelling periods of 4 and 6.5 h suggesting that the lag phase of incorporation of newly synthesized PSD proteins is sufficiently rapid to not produce this result artefactually. When the brain from a non-labelled chicken was homogenized in the presence of the S3 fraction from a labelled chicken and sub-fractionated the relative specific activities of the SPM and SJ fractions produced were 1-2% of those from the labelled brain. These results support the notion that tubulin and actin are intrinsic components of the PSD.

Citations Scopus - 5
1986 Weinberger RP, Rostas JA, 'Subcellular distribution of a calmodulin-dependent protein kinase activity in rat cerebral cortex during development.', Brain research, 394 37-50 (1986)

The postnatal development of calmodulin-stimulated phosphorylation of endogenous proteins, in particular the autophosphorylated subunits of the calmodulin-stimulated protein kinas... [more]

The postnatal development of calmodulin-stimulated phosphorylation of endogenous proteins, in particular the autophosphorylated subunits of the calmodulin-stimulated protein kinase II, were investigated in subcellular fractions of rat cerebral cortex. The major subunit had a mol. wt. of 53,000 Da (designated 50 kDa) and the minor one a mol. wt. of 63,000 Da (designated 60 kDa). The 50-kDa subunit was found to be the only significant phosphoprotein in each fraction and throughout development at its molecular weight. However, the 60-kDa subunit was found to comigrate with other phosphoproteins that accounted for up to 15% of the radioactivity at this molecular weight and which differed between the fractions. 50-kDa autophosphorylation was found to be 3-fold greater in cytoplasmic fractions at day 10 and by adults was evenly distributed between membrane and cytoplasmic fractions. A similar pattern was also found for the total calmodulin-stimulated phosphorylation. Changes in autophosphorylation activity of the 50-kDa subunit were found to represent changes in kinase activity rather than alterations in phosphatase activity. In the membrane, this change was shown to be due to changes in the amount of enzyme. Although in the adult autophosphorylation activity is evenly distributed between membrane and soluble fractions, when differences in phosphatase activity and lack of autophosphorylation activity of the majority of post-synaptic density-associated kinase is taken into account, it is clear that the vast majority of the enzyme is membrane-bound. Phosphorylation of endogenous substrates paralleled the development of 50-kDa subunit autophosphorylation, most of which occurred between day 14 and day 30, a period which follows the most rapid phase of synaptogenesis. This pattern was different from that of the phosphorylation of myelin basic protein and two substrates of the calcium-phospholipid-dependent protein kinase. There was also a change in the ratio of autophosphorylation activity of the 50-kDa and 60-kDa subunits during development which appears to be due to a change in the amount of the subunits themselves. This ratio was the same in all fractions at any one age. We suggest that this change is due to the existence of at least two developmentally regulated isoenzymes in the cortex.

Citations Scopus - 7
1986 LEUNG WN, JEFFREY PL, ROSTAS JAP, 'EFFECT OF DENERVATION ON SARCOLEMMAL PROTEINS AND GLYCOPROTEINS OF FAST AND SLOW MAMMALIAN SKELETAL-MUSCLE', EXPERIMENTAL NEUROLOGY, 91 229-245 (1986)
DOI 10.1016/0014-4886(86)90064-6
Citations Scopus - 10Web of Science - 11
1986 ROSTAS JAP, WEINBERGER RP, DUNKLEY PR, 'MULTIPLE POOLS AND MULTIPLE FORMS OF CALMODULIN-STIMULATED PROTEIN-KINASE DURING DEVELOPMENT - RELATIONSHIP TO POSTSYNAPTIC DENSITIES', PROGRESS IN BRAIN RESEARCH, 69 355-371 (1986)
DOI 10.1016/S0079-6123(08)61070-5
Citations Scopus - 22Web of Science - 26
Co-authors Peter Dunkley
1986 WEINBERGER RP, ROSTAS JAP, 'SUBCELLULAR-DISTRIBUTION OF A CALMODULIN-DEPENDENT PROTEIN-KINASE ACTIVITY IN RAT CEREBRAL-CORTEX DURING DEVELOPMENT', DEVELOPMENTAL BRAIN RESEARCH, 29 37-50 (1986)
DOI 10.1016/0165-3806(86)90080-5
Citations Scopus - 22Web of Science - 32
1986 SEDMAN GL, JEFFREY PL, AUSTIN L, ROSTAS JAP, 'THE METABOLIC TURNOVER OF THE MAJOR PROTEINS OF THE POSTSYNAPTIC DENSITY', MOLECULAR BRAIN RESEARCH, 1 221-230 (1986)
DOI 10.1016/0169-328X(86)90028-8
Citations Scopus - 8Web of Science - 10
1986 ROSTAS JAP, BRENT VA, HEATH JW, NEAME RLB, POWIS DA, WEINBERGER RP, DUNKLEY PR, 'THE SUBCELLULAR-DISTRIBUTION OF A MEMBRANE-BOUND CALMODULIN-STIMULATED PROTEIN-KINASE', NEUROCHEMICAL RESEARCH, 11 253-268 (1986)
DOI 10.1007/BF00967973
Citations Scopus - 19Web of Science - 23
Co-authors Peter Dunkley
1985 JEFFREY PL, LEUNG WN, ROSTAS JAP, 'THE EFFECT OF TETRODOTOXIN ON MAMMALIAN SARCOLEMMAL PROTEINS AND GLYCOPROTEINS', BIOCHEMISTRY INTERNATIONAL, 10 715-722 (1985)
1984 LEUNG WN, JEFFREY PL, ROSTAS JAP, 'THE EFFECT OF DENERVATION ON MAMMALIAN SARCOLEMMAL PROTEINS AND GLYCOPROTEINS', MUSCLE & NERVE, 7 35-49 (1984)
DOI 10.1002/mus.880070107
Citations Scopus - 7Web of Science - 12
1984 ROSTAS JAP, BRENT VA, GULDNER FH, 'THE MATURATION OF POST-SYNAPTIC DENSITIES IN CHICKEN FOREBRAIN', NEUROSCIENCE LETTERS, 45 297-304 (1984)
DOI 10.1016/0304-3940(84)90242-8
Citations Scopus - 21Web of Science - 24
1984 SEDMAN GL, JEFFREY PL, ROSTAS JAP, 'THE TURNOVER RATES OF CHICK BRAIN SYNAPTIC JUNCTION PROTEINS', PROCEEDINGS OF THE AUSTRALIAN BIOCHEMICAL SOCIETY, 16 32-32 (1984)
1984 WEINBERGER RP, DUNKLEY PR, ROSTAS JAP, 'DEVELOPMENTAL ACCUMULATION OF A CALMODULIN STIMULATED PROTEIN-KINASE IN SYNAPTIC-MEMBRANES', PROCEEDINGS OF THE AUSTRALIAN BIOCHEMICAL SOCIETY, 16 87-87 (1984)
Citations Web of Science - 1
Co-authors Peter Dunkley
1983 ROSTAS JAP, SHEVENAN TA, SINCLAIR CM, JEFFREY PL, 'THE PURIFICATION AND CHARACTERIZATION OF A THY-1-LIKE GLYCOPROTEIN FROM CHICKEN BRAIN', BIOCHEMICAL JOURNAL, 213 143-152 (1983)
Citations Scopus - 33Web of Science - 41
1983 ROSTAS JAP, BRENT VA, DUNKLEY PR, 'THE MAJOR CALMODULIN-STIMULATED PHOSPHOPROTEIN OF SYNAPTIC JUNCTIONS AND THE MAJOR POST-SYNAPTIC DENSITY PROTEIN ARE DISTINCT', NEUROSCIENCE LETTERS, 43 161-165 (1983)
DOI 10.1016/0304-3940(83)90181-7
Citations Scopus - 14Web of Science - 18
Co-authors Peter Dunkley
1982 LEUNG WN, JEFFREY PL, ROSTAS JAP, 'DENERVATION EXPOSES CRYPTIC CONCANAVALIN A BINDING-SITES IN SKELETAL-MUSCLE MEMBRANES', NEUROSCIENCE LETTERS, 30 31-36 (1982)
DOI 10.1016/0304-3940(82)90007-6
Citations Scopus - 7Web of Science - 8
1981 LEUNG WN, ROSTAS JAP, JEFFREY PL, 'PLASTICITY OF SKELETAL-MUSCLE MEMBRANES IN FOLLOWING DENERVATION', PROCEEDINGS OF THE AUSTRALIAN BIOCHEMICAL SOCIETY, 14 118-118 (1981)
1981 ROSTAS JAP, LEUNG WN, JEFFREY PL, 'GLYCOSYLTRANSFERASE ACTIVITIES IN CHICKEN BRAIN SYNAPTIC JUNCTIONS', NEUROSCIENCE LETTERS, 24 155-160 (1981)
DOI 10.1016/0304-3940(81)90240-8
Citations Scopus - 6Web of Science - 6
1981 ROSTAS JAP, JEFFREY PL, 'MATURATION OF SYNAPSES IN CHICKEN FOREBRAIN', NEUROSCIENCE LETTERS, 25 299-304 (1981)
DOI 10.1016/0304-3940(81)90408-0
Citations Scopus - 12Web of Science - 16
1980 ROSTAS JAP, SHEVENAN TA, JEFFREY PL, 'A DEVELOPMENTALLY REGULATED THY-1-LIKE MEMBRANE GLYCOPROTEIN IN CHICKENS', PROCEEDINGS OF THE AUSTRALIAN BIOCHEMICAL SOCIETY, 13 119-119 (1980)
Citations Web of Science - 1
1979 ROSTAS JAP, KELLY PT, PESIN RH, COTMAN CW, 'PROTEIN AND GLYCOPROTEIN COMPOSITION OF SYNAPTIC JUNCTIONS PREPARED FROM DISCRETE SYNAPTIC REGIONS AND DIFFERENT SPECIES', BRAIN RESEARCH, 168 151-167 (1979)
DOI 10.1016/0006-8993(79)90133-1
Citations Scopus - 52Web of Science - 67
1979 ROSTAS JAP, AUSTIN L, JEFFREY PL, 'SELECTIVE LABELING OF 2 PHASES OF AXONAL-TRANSPORT OF CHOLESTEROL IN THE CHICK OPTIC-SYSTEM', JOURNAL OF NEUROCHEMISTRY, 32 1461-1466 (1979)
DOI 10.1111/j.1471-4159.1979.tb11085.x
Citations Scopus - 5Web of Science - 7
1979 LEUNG WN, ROSTAS JAP, JEFFREY PL, 'ALTERATIONS IN SURFACE-MEMBRANES OF MAMMALIAN SKELETAL-MUSCLE FOLLOWING DENERVATION', PROCEEDINGS OF THE AUSTRALIAN BIOCHEMICAL SOCIETY, 12 93-93 (1979)
1979 JEFFREY PL, LEUNG WN, ROSTAS JAP, 'DENERVATION ALTERATIONS IN SURFACE-MEMBRANES OF MAMMALIAN SKELETAL-MUSCLE', MUSCLE & NERVE, 2 310-310 (1979)
1977 ROSTAS JAP, KELLY PT, COTMAN CW, 'IDENTIFICATION OF MEMBRANE GLYCO-COMPONENTS IN POLYACRYLAMIDE GELS - RAPID METHOD USING I-125-LABELED LECTINS', ANALYTICAL BIOCHEMISTRY, 80 366-372 (1977)
DOI 10.1016/0003-2697(77)90657-1
Citations Scopus - 29Web of Science - 49
1977 HOWE PRC, FENWICK EM, ROSTAS JAP, LIVETT BG, 'IMMUNOCHEMICAL COMPARISON OF SYNAPTIC PLASMA-MEMBRANE AND SYNAPTIC VESICLE MEMBRANE ANTIGENS', JOURNAL OF NEUROCYTOLOGY, 6 339-352 (1977)
DOI 10.1007/BF01175195
Citations Scopus - 7Web of Science - 10
Co-authors Peter Howe
1977 ROSTAS JAP, JEFFREY PL, 'IMMUNOCHEMICAL CHARACTERIZATION OF SYNAPTOSOMAL MEMBRANE ANTIGENS FROM CHICKEN BRAIN - HISTOCHEMICAL LOCALIZATION IN DAY-OLD CHICK', NEUROCHEMICAL RESEARCH, 2 59-85 (1977)
DOI 10.1007/BF00966022
Citations Scopus - 5Web of Science - 8
1977 ROSTAS JAP, JEFFREY PL, 'IMMUNOCHEMICAL CHARACTERIZATION OF SYNAPTOSOMAL MEMBRANE ANTIGENS FROM CHICKEN BRAIN - HISTOCHEMICAL DEMONSTRATION OF CHANGES IN DISTRIBUTION DURING DEVELOPMENT', NEUROCHEMICAL RESEARCH, 2 171-189 (1977)
DOI 10.1007/BF00964095
Citations Scopus - 4Web of Science - 3
1977 ROSTAS JAP, JEFFREY PL, 'IMMUNOCHEMICAL CHARACTERIZATION OF SYNAPTOSOMAL MEMBRANE ANTIGENS FROM CHICKEN BRAIN - QUANTITATIVE-DETERMINATION AND PARTIAL CHARACTERIZATION', NEUROCHEMICAL RESEARCH, 2 395-406 (1977)
DOI 10.1007/BF00965463
Citations Web of Science - 1
1975 ROSTAS JAP, MCGREGOR A, JEFFREY PL, 'TRANSPORT OF CHOLESTEROL IN CHICK OPTIC-SYSTEM', JOURNAL OF NEUROCHEMISTRY, 24 295-302 (1975)
DOI 10.1111/j.1471-4159.1975.tb11879.x
Citations Scopus - 13Web of Science - 14
1975 HOWE PRC, ROSTAS JAP, FENWICK EM, LIVETT BG, 'FURTHER EVIDENCE FOR INCORPORATION OF SYNAPTIC VESICLE PROTEINS INTO SYNAPTOSOMAL PLASMA-MEMBRANES', PROCEEDINGS OF THE AUSTRALIAN BIOCHEMICAL SOCIETY, 8 95-95 (1975)
Citations Web of Science - 1
Co-authors Peter Howe
1975 ROSTAS JAP, JEFFREY PL, 'ANTIGENIC MEMBRANE COMPONENTS IN DEVELOPING NERVE-CELLS', PROCEEDINGS OF THE AUSTRALIAN BIOCHEMICAL SOCIETY, 8 117-117 (1975)
1975 ROSTAS JAP, JEFFREY PL, 'RESTRICTED MOBILITY OF NEURONAL MEMBRANE ANTIGENS', NEUROSCIENCE LETTERS, 1 47-53 (1975)
DOI 10.1016/0304-3940(75)90011-7
Citations Scopus - 3Web of Science - 4
1974 LIVETT BG, ROSTAS JAP, JEFFREY PL, AUSTIN L, 'ANTIGENICITY OF ISOLATED SYNAPTOSOMAL MEMBRANES', EXPERIMENTAL NEUROLOGY, 43 330-338 (1974)
DOI 10.1016/0014-4886(74)90174-5
Citations Scopus - 20Web of Science - 24
1974 ROSTAS JAP, MCGREGOR A, JEFFREY PL, AUSTIN L, 'CHOLESTEROL TRANSPORT IN CRANIAL AND PERIPHERAL-NERVES', PROCEEDINGS OF THE AUSTRALIAN BIOCHEMICAL SOCIETY, 7 35-35 (1974)
1973 ROSTAS JAP, JEFFREY PL, 'UNUSUAL DISRUPTION OF SYNAPTOSOMES BY FRENCH PRESS TREATMENT', PROCEEDINGS OF THE AUSTRALIAN BIOCHEMICAL SOCIETY, 6 46-46 (1973)
Show 140 more journal articles

Conference (27 outputs)

Year Citation Altmetrics Link
2015 Rostas J, Hoffman A, Murtha L, Pepperall D, McLeod D, Dickson P, et al., 'Ischaemia-induced neuronal cell death is mediated by molecular targeting of CaMKII phosphorylated at T253', JOURNAL OF NEUROCHEMISTRY (2015) [E3]
Co-authors Kathryn Skelding, Lucy Murtha, Neil Spratt, Phil Dickson, Damian Mcleod
2012 Skelding KA, Abdul Majeed ABB, Dickson PW, Spratt NJ, Rostas JA, 'CAMKII is regulated differently in brains regions with differing sensitivities to ischaemia/excitotoxicity', Abstracts. Australian Neuroscience Society 32nd Annual Meeting (2012) [E3]
Co-authors Phil Dickson, Kathryn Skelding, Neil Spratt
2012 Skelding KA, Dickson PW, Verrills NM, Rostas JA, 'Dephosphorylation of CAMKII at T253 controls progression through metaphase', Abstracts. Australian Neuroscience Society 32nd Annual Meeting (2012) [E3]
Co-authors Nikki Verrills, Phil Dickson, Kathryn Skelding
2012 Abdul Majeed ABB, Skelding KA, Dickson PW, Rostas JA, 'Does phosphorylation of CaMKII at multiple sites regulate CaMKII targeting co-operatively or independently?', Abstracts. Australian Neuroscience Society 32nd Annual Meeting (2012) [E3]
Co-authors Phil Dickson, Kathryn Skelding
2012 Rostas JA, Skelding KA, Fluechter L, Dickson PW, Spratt NJ, 'CaMKII is differentially regulated in striatum and cortex', Journal of Molecular Neuroscience: Abstracts The 21st Annual Meeting of the Israel Society for Neuroscience & The First Binational Australian-Israeli Meeting in Neuroscience (2012) [E3]
Co-authors Phil Dickson, Neil Spratt, Kathryn Skelding
2011 Skelding KA, Chung S, Pepperall D-G, Tomkins AJ, Spratt NJ, Rostas JA, 'The role of CaMKII in neuronal sensitivity to ischaemia', Oral abstracts. Australian Neuroscience Society Annual Meeting (2011) [E3]
Co-authors Kathryn Skelding, Neil Spratt
2010 Rostas JA, Skelding KA, 'The role of targeting in the regulation of CaMKII in vivo', OzBio 2010: The Molecules of Life - from Discovery to Biotechnology Symposium Abstracts (2010) [E3]
Co-authors Kathryn Skelding
2010 Skelding KA, Tomkins AJ, Fluechter L, Pepperall D-G, Spratt NJ, Rostas JA, 'Ischaemia-induced CaMKII phosphorylation in hypertensive and normotensive rats', Proceding of the Australian Neuroscience Society (2010) [E3]
Co-authors Neil Spratt, Kathryn Skelding
2010 Skelding KA, Verrills NM, Dickson PW, Rostas JA, 'Regulation of proliferation of neuroblastoma cells by CaMKII', Proceding of the Australian Neuroscience Society (2010) [E3]
Co-authors Nikki Verrills, Kathryn Skelding, Phil Dickson
2010 Skelding KA, Xue J, Suzuki T, Verrills NM, Dickson PW, Rostas JA, 'Mechanisms of phosphorylation-sensitive CaMKII targeting', Proceding of the Australian Neuroscience Society (2010) [E3]
Co-authors Phil Dickson, Nikki Verrills, Kathryn Skelding
2009 Skelding KA, Liao X, Verrills NM, Fluechter L, Sim AT, Dickson PW, Rostas JA, 'Functional consequences of CaMKII phosphorylation at THR253 in neurons', ANS 2009 Abstracts: Posters (2009) [E3]
Co-authors Alistair Sim, Phil Dickson, Nikki Verrills, Kathryn Skelding
2009 Tomkins AJ, Rostas JA, Pepperall D-G, Calford MB, Spratt NJ, 'Infarction occurs more rapidly in hypertensive rats', ANS 2009 Abstracts: Posters (2009) [E3]
Co-authors Neil Spratt
2009 Rostas JA, 'Regulation of CaMKII activity', ANS 2009 Abstracts: Symposia (2009) [E3]
2009 Rostas JA, Skelding KA, Liao X, Verrills NM, Dickson PW, 'Regulation of CaMKII by targeting', Proceedings of the 2nd Australia-China Biomedical Research Conference (2009) [E3]
Co-authors Phil Dickson, Nikki Verrills, Kathryn Skelding
2008 Skelding KA, Verrills NM, Fluechter L, Sim AT, Dickson PW, Rostas JA, 'Identification of CaMKII binding proteins in brain sensitive to CaMKII phosphorylation state', Proceedings of the Australian Neuroscience Society (2008) [E3]
Co-authors Alistair Sim, Phil Dickson, Nikki Verrills, Kathryn Skelding
2007 Hunter M, Atkinson RJ, Rostas JA, 'Perturbed maturation in chicken forebrain via the induction of mild hypothyroidism: developmental changes measured by auditory event related potentials (Poster)', 7th IBRO 2007 World Congress of Neuroscience Program (2007) [E3]
Co-authors Mick Hunter
2007 Figliola A, Serrano E, Rostas JA, Hunter M, Rosso OA, 'Study of EEG brain maturation signals with multifractal detrended fluctuation analysis', Nonequilibrium Statistical Mechanics and Nonlinear Physics: XV Conference on Nonequilibrium Statistical Mechanics and Nonlinear Physics. AIP Conference Proceedings (2007) [E1]
DOI 10.1063/1.2746746
Citations Scopus - 4Web of Science - 2
Co-authors Mick Hunter
2007 Fernandez JG, Larrondo HA, Figliola A, Serrano E, Rostas JA, Hunter M, Rosso OA, 'Brain maturation changes characterized by algorithmic complexity (lempel and ziv complexity)', Nonequilibrium Statistical Mechanics and Nonlinear Physics: XV Conference on Nonequilibrium Statistical Mechanics and Nonlinear Physics. AIP Conference Proceedings (2007) [E1]
Citations Scopus - 4Web of Science - 5
Co-authors Mick Hunter
2006 Rostas JAP, Migues P, Lehmann IT, Fluechter L, Cammarota M, Gurd JW, et al., 'Phosphorylation of CAMKII at Thr253 occurs in vivo and enhances binding to isolated post synaptic densities', JOURNAL OF NEUROCHEMISTRY (2006)
Co-authors Alistair Sim, Phil Dickson
2006 Rostas JA, Migues PV, Fluechter L, Dickson PW, Sim AT, Rawoff S, et al., 'Phosphorylation of CAMKII at T253 induced by transient global ischaemia', Proceedings of the Australian Neuroscience Society (2006) [E3]
Co-authors Phil Dickson, Alistair Sim
2006 Atkinson RJ, Thomas (Duff) L, Migues PV, Fluechter L, Rostas JA, Hunter M, 'Perturbed maturation in the chicken forebrain by the induction of late onset mild hypo-thyroidsim', Proceedings of the Australian Neuroscience Society (2006) [E3]
Co-authors Mick Hunter
2005 Migues PV, Lehmann IT, Fluechter L, Cammarota M, Sim AT, Gurd JW, et al., 'Phosphorylation of CaMKII on T253 enhances its association with postsynaptic densities', JOURNAL OF NEUROCHEMISTRY (2005)
Co-authors Phil Dickson
2005 Rostas JA, 'New mechanisms of control of CaMKII: Regulating the regulator', JOURNAL OF NEUROCHEMISTRY (2005)
2005 Atkinson RJ, Migues PV, Fluechter L, Hunter M, Rostas JA, 'Biochemical characterisation of maturational changes in components of glutamatergic synapses in the chick IMHV', Australian Neuroscience Society: Proceedings of the 25th Annual (2005) [E3]
Co-authors Mick Hunter
2004 Rostas JA, Baldwin ML, Cammarota MP, Sim AT, 'The regulation of synaptosomal exocytosis by Src tyrosine kinases', Journal of Neurochemistry (2004) [E3]
Co-authors Alistair Sim
2004 Migues PV, Sim AT, Rostas JA, 'Changes in KCI-induced phosphorylation of AMPA and NMDA receptors during chicken brain maturation', Journal of Neurochemistry (2004) [E3]
Co-authors Alistair Sim
2003 Migues PV, Cammarota M, Fluechter L, Bissoon N, Gurd JW, Rostas JAP, 'A new phosphorylation site on CaMKII in vivo', JOURNAL OF NEUROCHEMISTRY (2003)
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Research Supervision

Number of supervisions

Completed7
Current0

Past Supervision

Year Level of Study Research Title / Program / Supervisor Type
2013 Masters Effects of Multi-site Phosphorylation on CaMKII Function
M Philosophy (Medical Biochem), Faculty of Health and Medicine, The University of Newcastle
Principal Supervisor
2008 PhD Brain Maturation in Chickens: Biochemical, Behavioural and Electrophysiological Investigations
PhD (Psychology - Science), Faculty of Science, The University of Newcastle
Co-Supervisor
2004 PhD Differential Regulation of Two Modes of Synaptic Vesicle Exocytosis by Protein Phosphorylation
PhD (Medical Biochemistry), Faculty of Health and Medicine, The University of Newcastle
Co-Supervisor
2000 PhD Identification of brain proteins whose phosphorylation is regulated by NO and cGMP
Biochemistry & Cell Biology, University of Newcastle
Principal Supervisor
1998 PhD Synaptic plasticity in chicken hippocampus.
Biochemistry & Cell Biology, University of Newcastle
Principal Supervisor
1996 PhD Mechanisms responsible for neuronal compensation following unilateral labarynthectomy in the guinea pig.
Behavioural Science N.E.C., Unknown
Co-Supervisor
1996 PhD . Changes in glutamate receptors in chicken forebrain during maturation.
Biochemistry & Cell Biology, University of Newcastle
Principal Supervisor
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Emeritus Professor John Rostas

Position

Emeritus Professor
Faculty of Health and Medicine

Contact Details

Email john.rostas@newcastle.edu.au
Phone (02) 4921 5615
Fax (02) 4921 7903

Office

Room LS3-40
Building Life Sciences
Location Callaghan
University Drive
Callaghan, NSW 2308
Australia
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