
Professor Brett Nixon
NHMRC Research Fellow
School of Environmental and Life Sciences (Biological Sciences)
- Email:brett.nixon@newcastle.edu.au
- Phone:(02) 4921 6977
Spot the difference
An investigator of indomitable spirit, Professor Brett Nixon is pursuing the mysteries of sperm dysfunction from multiple, linked perspectives.
What do adult humans, wild rabbits, platypus and Australian saltwater crocodiles have in common? Plenty, according to Professor Brett Nixon. The esteemed educator and innovator studies all four seemingly disparate populations in his research, shedding light on the surprising connections between male factor infertility, contraception and the conservation of endangered animals.
"It's almost paradoxical," he remarks.
"If we can understand the cellular defects present in the sperm of infertile males, then we may be able to develop methods to replicate them in pest animal species to create a new form of contraceptive."
"Physiological insights provided by studying these cells can help us augment the success of captive breeding too."
Though there are "countless things" that can go wrong during the process of fertilisation, Brett is opting to focus mainly on its beginning. Comparing and contrasting samples of sperm that are able to bind to eggs with samples of sperm that can't, the proteomics expert is working to get a grasp on the structure, function and interactions of proteins that differ between the two.
"I specifically look for the proteins that are missing or appear to be behaving abnormally," he explains.
"They may hold a key role in helping to define the underlying cause of male infertility."
Spanning the life sciences and medicine fields, Brett's efforts are also concentrated on improving artificial reproductive technologies (ARTs).
"This long term interest stems from early childhood experiences in which my family were using ARTs to improve the quality of our herd of beef and dairy cattle," the Gloucester native recalls.
"The basic idea was that we would collect eggs from the good mothers and put them together with the semen from the best bulls from around the world."
"The embryos generated were subsequently transplanted into surrogates."
"I still remember looking down a microscope for the first time and witnessing sperm binding to eggs and kicking off the fertilisation process – it was the most amazing sight."
The ties that bind
Brett's career began in 1995, when he undertook a PhD at the University of Newcastle. Completed in Canberra at the Invasive Animal CRC, the ambitious four-year probe centred on figuring out ways to sterilise foxes, rabbits and mice.
"I identified a handful of core proteins involved in a sperm's recognition of an egg and tried to raise an immune response against them to prevent fertilisation from happening," he elaborates.
"This approach was essentially trying to recapitulate a phenomenon seen in a subset of male infertility patients whereby their immune system attacks their sperm and prevents them from binding to eggs."
Seeking to continue his research on male factor infertility, Brett spent the next two years at Emory University in the United States.
"By the time I arrived, scholars within its Department of Cell Biology had developed a mouse model in which they had knocked out one of the key proteins believed to be involved in sperm-egg recognition," he states.
"So in theory, as the mouse can no longer produce that protein, its sperm shouldn't be able to recognise the egg."
"Yet it turns out they were still fully fertile."
Disheartened but not defeated, Brett and his colleagues looked to unravel this improbable twist.
"We started to dissect how a critical element could be eliminated and the sperm still retain its ability to initiate fertilisation," he comments.
"What we have since learnt is that there is likely a lot of redundancy in that initial interaction, so if one protein is removed from the equation, others are available to stand up and take its place."
"There must be a handful that participate, a reflection of the overall importance of this event in the initiation of a new life."
Mechanics and Mother Nature
Brett returned to Australia in 2001, signing on to work alongside Laureate Professor John Aitken at the University of Newcastle. "Too good and exciting an opportunity" to pass up, the past 14 years have been some of the Australian Research Council Future Fellow's most memorable – and productive.
"My team recently conducted a global analysis of the proteins present in fertile and infertile patients," he shares.
"Collaborating with Dr Mark Baker, I came up with a small number of proteins that change consistently between the two."
"At the moment I'm focusing on just one, however."
"We think it's the master regulator that primes the cell for its interactions with the egg."
Faithful to his pastoral origins, Brett has also put in a grant to build up research on sperm-egg recognition in other species. Currently in the process of collecting pilot data for the project, the multitasking academic is specifically interested in applying ARTs to improve the quality of saltwater crocodiles bred at Rockhampton's Koorana Crocodile Farm.
"There is an inherent danger in putting a male crocodile with a female."
"They may fight, resulting in the loss of one or both animals."
"It would be a real advance for the industry if we could just house the latter as is now standard practice in other livestock industries."
Additionally concerned about conservation, Brett is also teaming up with Taronga Zoo on this project.
"We might be able to contribute to improving the success of captive breeding of crocodilian species, many of which are currently threatened or endangered," he suggests.
Asking the big questions
Brett is simultaneously endeavouring to understand why mammals have developed such a "complicated" fertilisation process. Fascinated from an "evolutionary point of view," he's particularly intrigued by the similarities and differences in sperm maturation events that happen in species such as platypus and echidnas versus that of higher mammals and other vertebrates.
"In our own species, sperm maturation takes place over several weeks!" he declares.
"The cells that leave the testes are functionally immature and do not gain the ability to engage in fertilisation until they travel through the male and female reproductive tracts."
"We don't yet know why this is and it is particularly curious given that in species such as the birds, sperm come out of the testes ready to go."
Always willing to lend a helping hand, Brett is not short of praise when it comes to talking about his predecessors and successors either.
"Laureate Professor John Aitken, Conjoint Professor Russell Jones and Professor Eileen McLaughlin have been inspiring mentors," he muses,
"They've pointed me in the right direction when things were wavering."
"I also count myself very fortunate to work with a wonderful group of students, research assistants, and academics."
"One of the most rewarding aspects of my job is the interaction I have with these individuals and watching my students develop research careers of their own."
Spot the difference
An investigator of indomitable spirit, Professor Brett Nixon is pursuing the mysteries of sperm dysfunction from multiple, linked perspectives
Career Summary
Biography
Research Expertise
I obtained my PhD from the University of Newcastle (awarded 1999) working on a collaborative project with the Vertebrate Biocontrol Cooperative Research Centre focused on characterising protein expression and function in male gametogenesis. This work helped to define to role of the male reproductive tract in post-testicular sperm maturation. I then undertook post-doctoral research in the Department of Cell Biology, Emory University, GA, USA (1999-2000). This work centred on the characterisation of knockout mouse models bearing targeted deletions of a key proteins involved in gamete interactions. In 2001, I was recruited to the University of Newcastle as an Associate Lecturer in Biological Sciences and promoted to the position of Lecturer in 2004, Senior Lecturer in 2007, Associate Professor in 2011, and Professor in 2016. Since appointment I have established an active research program focusing on the use of human and animal models to characterise the processes of gamete maturation. In spermatozoa, I am currently working on a number of key molecules that control cell function and regulate gamete interactions. In folliculogenesis, I have focused on cell signalling pathways that contribute to the initiation of primordial follicle activation and the effects of environmental ovotoxicants on these pathways. These projects are of strategic importance in the design of post-testicular methods of fertility regulation, the development of assisted conception systems for the preservation of endangered species and elucidation of the causes of human male infertility. My research niche complements existing programs in male germ cell development and ovarian biology within the Reproductive Science Group at the University of Newcastle. It also represents a strategically important subprogram of the University’s Priority Research Centre (PRC) for Reproductive Science.
Teaching Expertise
Undergraduate Teaching Since my appointment in 2001, I have made a significant and sustained contribution to the teaching effort of the Discipline of Biological Sciences through the delivery of lectures and supervision of practical classes and tutorials across a broad range of courses (Levels 1000 – 4000). I strive for excellence in teaching and generate consistently high SEC and SET evaluations and qualitative feedback. My current teaching commitment includes: BIOL1001 Cells, Molecules, and Organisms BIOL1001 Gifted and Talented Program (Course coordinator) BIOL2001 Molecular Laboratory Skills for Biological Sciences BIOL2010 Biochemistry (Course coordinator) BIOL2050 Molecular Genetics BIOL3200 Cellular Biotechnology BIOL3250 Biotechnology Placement BIOL4110 - BIOL4220 BSc (Biology) Honours / BBiotech Honours (Program coordinator) Research Training Since 2001, I have supervised 6 PhD, 1 MPhil and 13 Honours students to successful completion of their research higher degrees. I am currently the principal supervisor of 3 PhD and 1 Honours student and the co-supervisor of an additional 3 PhD students. My commitment to research training is evidenced by the recent award of RHD Supervisor of the Year (2009), the University of Newcastle’s highest accolade for student supervision. The quality of my students is attested by the award of numerous platform and poster prizes at national and international meetings, including: best student poster at Lorne Protein (2003), Oozoa award for best student oral presentation at the Society for Reproduction (SRB) annual meeting (2008 & 2010), best student poster at the Australian Society for Biochemistry and Molecular Biology annual meeting (2010), best student poster at the International Symposium on Spermatology (Japan, 2010), and the SRB - New Investigator award (2011).
Administrative Expertise
Service to the University I am dedicated to building achievements in relation to University, Community and Professional Service. Since appointment to my current position I have made a significant commitment to University Service and related professional activities. Specifically, I focused on initiatives that enrich the student learning experience and promote a vibrant research culture, thus contributing to the status of the University's teaching and research profile. My committee membership aligns with my recognised strengths in teaching and research, and has included: Assistant Dean Teaching and Learning, Faculty of Science and IT Student Recruitment Committee, Faculty of Science and IT Honours Committee Chair, School of Environmental and Life Sciences Postgraduate Committee, Discipline of Biological Sciences Analytical and Biomolecular Research Facility Advisory Committee OH&S Committee, Reproductive Sciences Group Service to the profession Elected member of Council / Chair of Awards Committee for Society for Reproductive Biology (2014 - ongoing) Member of Grant Review Panel for Reproductive Medicine, Obstetrics & Gynaecology, and Paediatrics, National Health and Medical Research Council of Australia (2013-ongoing) Associate Editor, Molecular Human Reproduction (ranked 3/28 in Reproductive Biology) (2011 - ongoing) I review for a number of grant bodies such as NHMRC and Medical Research Council (UK). I also review for 20 specialist journals including Reproduction, Biology of Reproduction, Human Reproduction, Developmental Biology (~20 manuscripts/year).
Collaborations
I have collaborative partnerships with a number of pre-eminent national and international scientists within the field of gamete biology. Similarly, I have established collaborations with IVF clinics to support the extension of my research program into the clinical domain. This nexus is critical for our ability to drive translational outcomes in understanding the aetiology of human male infertility.
Qualifications
- PhD, University of Newcastle
- Bachelor of Science (Honours), University of Newcastle
- Bachelor of Science, University of Newcastle
Keywords
- Andrology
- Biology
- Gamete Biology
- Reproductive Biology
Professional Experience
UON Appointment
Title | Organisation / Department |
---|---|
Professor | University of Newcastle School of Environmental and Life Sciences Australia |
Professor | University of Newcastle School of Environmental and Life Sciences Australia |
Academic appointment
Dates | Title | Organisation / Department |
---|---|---|
1/1/2015 - | Fellow | ARC (Australian Research Council) |
1/1/2013 - | Elected member of Council / Chair of Awards Committee | Society for Reproductive Biology Australia |
1/1/2011 - | Editor - Molecular Human Reproduction | Molecular Human Reproduction Journal Australia |
1/1/2008 - | Membership - Society for the Study of Reproduction | Society for the Study of Reproduction Australia |
1/1/1997 - | Membership - Society for Reproductive Biology | Society for Reproductive Biology Australia |
1/1/1999 - 1/12/2000 | NIH Post-Doctoral Research Fellow | Emory University, School of Medicine, Atlanta, GA Department of Cell Biology United States |
1/7/2011 - | Associate Professor | University of Newcastle School of Environmental and Life Sciences Australia |
1/1/2008 - 1/6/2011 | Senior Lecturer | University of Newcastle School of Environmental and Life Sciences Australia |
1/1/2004 - 1/1/2008 | Lecturer | University of Newcastle School of Environmental and Life Sciences Australia |
1/1/2001 - 1/1/2004 | Associate Lecturer | University of Newcastle School of Environmental and Life Sciences Australia |
Professional appointment
Dates | Title | Organisation / Department |
---|---|---|
1/1/2017 - 1/1/2021 |
Editorial Board Member Editorial Board Member |
Asian Journal of Andrology China |
1/1/2017 - 1/1/2021 |
Associate Editor Associate Editor |
Frontiers in Endocrinology (Specialty Section - Reproduction) Switzerland |
1/1/2016 - 31/12/2018 |
Co-chair Program Organising Committee Co-chair Program Organising Committee |
Society for Reproductive Biology Australia |
Awards
Award
Year | Award |
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2019 |
Society for Reproductive Biology Presidents Lecturer Society for Reproductive Biology |
Distinction
Year | Award |
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2015 |
Fellow of the Society for Reproductive Biology Society for Reproductive Biology |
2013 |
Vice-Chancellor’s Award for Teaching Excellence and Learning Support University of Newcastle |
2013 |
Vice-Chancellor’s citation for outstanding contributions to student learning University of Newcastle |
2009 |
Faculty of Science and IT Research Higher Degree Supervisor of the Year Unknown |
2009 |
The University of Newcastle Supervisor of the Year Unknown |
Prize
Year | Award |
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2018 |
Excellent Editorial Board Member Award Asian Journal of Andrology |
2017 |
Outstanding Paper Award Asian Journal of Andrology |
Recipient
Year | Award |
---|---|
2014 |
Citation for Outstanding Contributions to Student Learning Office for Learning and Teaching |
Recognition
Year | Award |
---|---|
2011 |
Certificate of Recognition for excellent contribution to peer review Unknown |
1998 |
Finalist, Junior Scientist Competition Unknown |
Research Award
Year | Award |
---|---|
2012 |
Award for Excellence in Reproductive Biology Research Society for Reproductive Biology |
2007 |
New Investigator Grant Hunter Medical Research Institute |
2006 |
Lalor Foundation Award Unknown |
1998 |
Student Travel Grant Unknown |
Invitations
Participant
Year | Title / Rationale |
---|---|
2007 |
The Biology of Extracellular Molecular Chaperones Organisation: Novartis Foundation |
Publications
For publications that are currently unpublished or in-press, details are shown in italics.
Chapter (12 outputs)
Year | Citation | Altmetrics | Link | ||||||||
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2020 |
Nixon B, Bromfield E, 'New Horizons in Male Subfertility and Infertility', Male and Sperm Factors that Maximize IVF Success, Cambridge University Press, Cambridge, UK (2020)
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2019 |
Gould T, Jamaluddin M, Petit J, King SJ, Nixon B, Scott R, et al., 'Finding Needles in Haystacks: The Use of Quantitative Proteomics for the Early Detection of Colorectal Cancer', Advances in the Molecular Understanding of Colorectal Cancer, IntechOpen, Switzerland 1-32 (2019) [B1]
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2018 |
Jones RC, Nixon B, Grützner F, 'Reproduction in Monotremes', Encyclopedia of Reproduction, Academic Press, New York City, New York, United States 602-608 (2018) [B1]
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2017 |
Nixon B, Bromfield EG, Cui J, De Iuliis GN, 'Heat shock protein A2 (HSPA2): regulatory roles in germ cell development and sperm', The Role of Heat Shock Proteins in Reproductive System Development and Function, Springer International Publishing, Cham, Switzerland 67-93 (2017) [B1]
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2017 |
Nixon B, Dun MD, Aitken RJ, 'Proteomic Analysis of Human Spermatozoa', Immune Fertility: Impact of Immune Reactions on Human Fertility, Springer Nature, Switzerland 3-22 (2017) [B1]
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2016 |
Holt JE, Stanger SJ, Nixon B, McLaughlin EA, 'Non-coding RNA in spermatogenesis and epididymal maturation', Advances in Experimental Medicine and Biology, Springer, Dordrecht 95-120 (2016) [B1]
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2012 |
Redgrove KA, Aitken RJ, Nixon B, 'More than a simple lock and key mechanism: unraveling the intricacies of sperm-oocyte interactions', Binding Protein, InTech, Rijeka, Croatia 73-122 (2012) [B1]
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2010 |
Aitken RJ, Baker MA, De Iuliis GN, Nixon B, 'New insights into sperm physiology and pathology', Fertility Control, Springer, Berlin 99-115 (2010) [B1]
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2010 |
Dun MD, Mitchell LA, Aitken RJ, Nixon B, 'Sperm-zona pellucida interaction: Molecular mechanisms and the potential for contraceptive intervention', Fertility Control, Springer, Berlin 139-178 (2010) [B1]
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Show 9 more chapters |
Journal article (142 outputs)
Year | Citation | Altmetrics | Link | ||||||||
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2020 |
Liu G, Baird AW, Parsons MJ, Fan K, Skerrett-Byrne DA, Nair PM, et al., 'Platelet activating factor receptor acts to limit colitis-induced liver inflammation', FASEB JOURNAL, 34 7718-7732 (2020) [C1]
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2020 |
Nixon B, Cafe SL, Eamens AL, De Iuliis GN, Bromfield EG, Martin JH, et al., 'Molecular insights into the divergence and diversity of post-testicular maturation strategies', Molecular and Cellular Endocrinology, 517 110955-110955 (2020) [C1]
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2020 |
Fennell KA, Busby RGG, Li S, Bodden C, Stanger SJ, Nixon B, et al., 'Limitations to intergenerational inheritance: subchronic paternal stress preconception does not influence offspring anxiety', Scientific Reports, 10 (2020) [C1]
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2020 |
Aitken RJ, De Iuliis GN, Nixon B, 'The Sins of Our Forefathers: Paternal Impacts on De Novo Mutation Rate and Development', Annual Review of Genetics, 54 1-24 (2020) [C1]
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2020 |
Griffin RA, Swegen A, Baker M, Aitken RJ, Skerrett-Byrne DA, Rodriguez AS, et al., 'Mass spectrometry reveals distinct proteomic profiles in high- And low-quality stallion spermatozoa', Reproduction, 160 695-707 (2020) [C1] © 2020 Society for Reproduction and Fertility The horse breeding industry relies upon optimal stallion fertility. Conventional sperm assessments provide limited information regard... [more] © 2020 Society for Reproduction and Fertility The horse breeding industry relies upon optimal stallion fertility. Conventional sperm assessments provide limited information regarding ejaculate quality and are not individually predictive of fertilizing potential. The aim of this study was to harness mass spectrometry to compare the proteomic profiles of high- and low-quality stallion spermatozoa, with the ultimate goal of identifying fertility biomarker candidates. Extended stallion semen (n = 12) was fractionated using Percoll density gradients to isolate low-quality and high-quality sperm populations. Motility and morphological assessments were carried out, and proteomic analyses was conducted using UHPLC-MS/MS. High-quality spermatozoa recorded higher total (95.2 ± 0.52% vs 70.6 ± 4.20%; P = 0.001) and progressive motilities (43.4 ± 3.42% vs 27.3 ± 4.32%; P = 0.05), and a higher proportion of morphologically normal cells (50.2 ± 4.34% vs 38.8 ± 2.72%; P = 0.05). In total, 1069 proteins were quantified by UHPLC-MS/MS, of which 22 proteins were significantly more abundant in the high-quality sperm population (P = 0.05). A-kinase anchor protein 4 (AKAP4) and Hexokinase 1 (HK1) were considered possible biomarker candidates and their differential expression was confirmed by immunoblot. Protein expression was significantly correlated with total (AKAP4 R2 = 0.38, P = 0.01; HK1 R2 = 0.46, P = 0.001) and progressive motilities (AKAP4 R2 = 0.51, P = 0.001; HK1 R2 = 0.55, P = 0.01), percentage rapid (AKAP4 R2 = 0.29, P = 0.05; HK1 R2 = 0.58, P = 0.001), straight-line velocity (HK1 R2 = 0.50, P = 0.01) and straightness (HK1 R2 = 0.40, P = 0.01). Furthermore, AKAP4 was highly susceptible to adduction by 4-hydroxynonenal (4HNE), which resulted in a global reduction in the phosphorylation profiles following capacitation. In conclusion, the proteomic profiles of high- and low-quality stallion spermatozoa differ substantially, and proteins such as AKAP4 and HK1 could serve as biomarkers of ejaculate quality.
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2020 |
Walters JLH, Gadella BM, Sutherland JM, Nixon B, Bromfield EG, 'Male Infertility: Shining a Light on Lipids and Lipid-Modulating Enzymes in the Male Germline', Journal of Clinical Medicine, 9 (2020) [C1]
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2020 |
Cafe SL, Nixon B, Dun MD, Roman SD, Bernstein IR, Bromfield EG, 'Oxidative Stress Dysregulates Protein Homeostasis within the Male Germ Line', Antioxidants and Redox Signaling, 32 487-503 (2020) [C1]
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2020 |
Campbell L, Cafe SL, Upton R, Doody JS, Nixon B, Clulow J, Clulow S, 'A model protocol for the cryopreservation and recovery of motile lizard sperm using the phosphodiesterase inhibitor caffeine', CONSERVATION PHYSIOLOGY, 8 (2020) [C1]
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2020 |
Peters AE, Mihalas BP, Bromfield EG, Roman SD, Nixon B, Sutherland JM, 'Autophagy in Female Fertility: A Role in Oxidative Stress and Aging', ANTIOXIDANTS & REDOX SIGNALING, 32 550-568 (2020) [C1]
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2020 |
Nowicka-bauer K, Nixon B, 'Molecular changes induced by oxidative stress that impair human sperm motility', Antioxidants, 9 (2020) [C1]
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2020 |
Tamessar CT, Trigg NA, Nixon B, Skerrett-Byrne DA, Sharkey DJ, Robertson SA, et al., 'Roles of male reproductive tract extracellular vesicles in reproduction', AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY, (2020) [C1]
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2020 |
Walters JLH, Anderson AL, Martins da Silva SJ, Aitken RJ, De Iuliis GN, Sutherland JM, et al., 'Mechanistic Insight into the Regulation of Lipoxygenase-Driven Lipid Peroxidation Events in Human Spermatozoa and Their Impact on Male Fertility.', Antioxidants (Basel), 10 (2020)
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2020 |
Mihalas BP, Redgrove KA, Bernstein IR, Robertson MJ, McCluskey A, Nixon B, et al., 'Dynamin 2-dependent endocytosis is essential for mouse oocyte development and fertility', FASEB Journal, 34 5162-5177 (2020) [C1]
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2020 |
Chu C, Yu L, Henry-Berger J, Ru Y-F, Kocer A, Champroux A, et al., 'Knockout of glutathione peroxidase 5 down-regulates the piRNAs in the caput epididymidis of aged mice.', Asian Journal of Andrology, 22 590-601 (2020) [C1]
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2020 |
Fraser BA, Miller K, Trigg NA, Smith ND, Western PS, Nixon B, Aitken RJ, 'A novel approach to nonsurgical sterilization; application of menadione-modified gonocyte-targeting M13 bacteriophage for germ cell ablation in utero', PHARMACOLOGY RESEARCH & PERSPECTIVES, 8 (2020) [C1]
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2020 |
Murray HC, Enjeti AK, Kahl RGS, Flanagan HM, Sillar J, Skerrett-Byrne DA, et al., 'Quantitative phosphoproteomics uncovers synergy between DNA-PK and FLT3 inhibitors in acute myeloid leukaemia.', Leukemia, (2020)
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2020 |
Dun MD, Mannan A, Rigby CJ, Butler S, Toop HD, Beck D, et al., 'Shwachman Bodian Diamond syndrome (SBDS) protein is a direct inhibitor of protein phosphatase 2A (PP2A) activity and overexpressed in acute myeloid leukaemia', Leukemia, 34 3393-3397 (2020) [C1]
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2020 |
Lord T, Nixon B, 'Metabolic Changes Accompanying Spermatogonial Stem Cell Differentiation', Developmental Cell, 52 399-411 (2020) [C1]
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2019 |
Nixon B, De Iuliis GN, Dun MD, Zhou W, Trigg NA, Eamens AL, 'Profiling of epididymal small non-protein-coding RNAs', Andrology, 7 669-680 (2019) [C1]
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2019 |
Martin JH, Aitken RJ, Bromfield E, Cafe SL, Sutherland JM, Frost ER, et al., 'Investigation into the presence and functional significance of proinsulin C-peptide in the female germline', Biology of Reproduction, 100 1275-1289 (2019) [C1]
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2019 |
Nixon B, De Iuliis GN, Hart HM, Zhou W, Mathe A, Bernstein IR, et al., 'Proteomic profiling of mouse epididymosomes reveals their contributions to post-testicular sperm maturation', Molecular and Cellular Proteomics, 18 S91-S108 (2019) [C1]
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2019 |
Gaikwad AS, Anderson AL, Merriner DJ, O'Connor AE, Houston BJ, Aitken RJ, et al., 'GLIPR1L1 is an IZUMO-binding protein required for optimal fertilization in the mouse.', BMC Biology, 17 (2019) [C1]
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2019 |
Houston BJ, Nixon B, McEwan KE, Martin J, King BV, Aitken RJ, De Iuliis GN, 'Whole-body exposures to radiofrequency-electromagnetic energy can cause DNA damage in mouse spermatozoa via an oxidative mechanism', Scientific Reports, 9 (2019) [C1]
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2019 |
Brown P, RELISH Consortium, Zhou Y, 'Large expert-curated database for benchmarking document similarity detection in biomedical literature search', Database, 2019 (2019) [C1]
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2019 |
Duchatel RJ, Jackson ER, Alvaro F, Nixon B, Hondermarck H, Dun MD, 'Signal Transduction in Diffuse Intrinsic Pontine Glioma', PROTEOMICS, 19 (2019) [C1]
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2019 |
Nixon B, Johnston SD, Skerrett-Byrne DA, Anderson AL, Stanger SJ, Bromfield EG, et al., 'Modification of Crocodile Spermatozoa Refutes the Tenet That Post-testicular Sperm Maturation Is Restricted To Mammals', MOLECULAR & CELLULAR PROTEOMICS, 18 S59-S76 (2019) [C1]
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2019 |
Nixon B, Bernstein IR, Cafe SL, Delehedde M, Sergeant N, Anderson AL, et al., 'A Kinase Anchor Protein 4 is vulnerable to oxidative adduction in male germ cells', Frontiers in Cell and Developmental Biology, 7 (2019) [C1]
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2019 |
Zhou W, Stanger SJ, Anderson AL, Bernstein IR, De Iuliis GN, McCluskey A, et al., 'Mechanisms of tethering and cargo transfer during epididymosome-sperm interactions.', BMC biology, 17 35-35 (2019) [C1]
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2019 |
Bromfield E, Walters JLH, Cafe S, Bernstein I, Stanger SR, Anderson AL, et al., 'Differential cell death decisions in the testis: evidence for an exclusive window of ferroptosis in round spermatids', Molecular Human Reproduction, 25 241-256 (2019) [C1]
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2019 |
Xavier MJ, Roman SD, Aitken RJ, Nixon B, 'Transgenerational inheritance: how impacts to the epigenetic and genetic information of parents affect offspring health', HUMAN REPRODUCTION UPDATE, 25 519-541 (2019) [C1]
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2019 |
Martin JH, Aitken RJ, Bromfield EG, Nixon B, 'DNA damage and repair in the female germline: contributions to ART.', Human Reproduction Update, 25 180-201 (2019) [C1]
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2019 |
Nixon B, Johnston SD, Skerrett-Byrne DA, Anderson AL, Stanger SJ, Bromfield EG, et al., 'Modification of Crocodile Spermatozoa Refutes the Tenet That Post-testicular Sperm Maturation Is Restricted To Mammals.', Mol Cell Proteomics, 18 Suppl 1 S58-S76 (2019)
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2019 |
Nixon B, De Iuliis GN, Hart HM, Zhou W, Mathe A, Bernstein IR, et al., 'Proteomic Profiling of Mouse Epididymosomes Reveals their Contributions to Post-testicular Sperm Maturation.', Mol Cell Proteomics, 18 Suppl 1 S91-S108 (2019)
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2019 |
Mihalas BP, Camlin NJ, Xavier MJ, Peters AE, Holt JE, Sutherland JM, et al., 'The small non-coding RNA profile of mouse oocytes is modified during aging', AGING-US, 11 2968-2997 (2019) [C1]
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2019 |
Xavier MJ, Nixon B, Roman SD, Scott RJ, Drevet JR, Aitken RJ, 'Paternal impacts on development: Identification of genomic regions vulnerable to oxidative DNA damage in human spermatozoa', Human Reproduction, 34 1876-1890 (2019) [C1] © 2019 The Author(s) 2019. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For permissions, pleas... [more] © 2019 The Author(s) 2019. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com. STUDY QUESTION: Do all regions of the paternal genome within the gamete display equivalent vulnerability to oxidative DNA damage? SUMMARY ANSWER: Oxidative DNA damage is not randomly distributed in mature human spermatozoa but is instead targeted, with particular chromosomes being especially vulnerable to oxidative stress. WHAT IS KNOWN ALREADY: Oxidative DNA damage is frequently encountered in the spermatozoa of male infertility patients. Such lesions can influence the incidence of de novo mutations in children, yet it remains to be established whether all regions of the sperm genome display equivalent susceptibility to attack by reactive oxygen species. STUDY DESIGN, SIZE, DURATION: Human spermatozoa obtained from normozoospermic males (n = 8) were split into equivalent samples and subjected to either hydrogen peroxide (H2O2) treatment or vehicle controls before extraction of oxidized DNA using a modified DNA immunoprecipitation (MoDIP) protocol. Specific regions of the genome susceptible to oxidative damage were identified by next-generation sequencing and validated in the spermatozoa of normozoospermic males (n = 18) and in patients undergoing infertility evaluation (n = 14). PARTICIPANTS/MATERIALS, SETTING, METHODS: Human spermatozoa were obtained from normozoospermic males and divided into two identical samples prior to being incubated with either H2O2 (5 mm, 1 h) to elicit oxidative stress or an equal volume of vehicle (untreated controls). Alternatively, spermatozoa were obtained from fertility patients assessed as having high basal levels of oxidative stress within their spermatozoa. All semen samples were subjected to MoDIP to selectively isolate oxidized DNA, prior to sequencing of the resultant DNA fragments using a next-generation whole-genomic sequencing platform. Bioinformatic analysis was then employed to identify genomic regions vulnerable to oxidative damage, several of which were selected for real-time quantitative PCR (qPCR) validation. MAIN RESULTS AND THE ROLE OF CHANCE: Approximately 9000 genomic regions, 150-1000 bp in size, were identified as highly vulnerable to oxidative damage in human spermatozoa. Specific chromosomes showed differential susceptibility to damage, with chromosome 15 being particularly sensitive to oxidative attack while the sex chromosomes were protected. Susceptible regions generally lay outside protamine-and histone-packaged domains. Furthermore, we confirmed that these susceptible genomic sites experienced a dramatic (2-15-fold) increase in their burden of oxidative DNA damage in patients undergoing infertility evaluation compared to normal healthy donors. LIMITATIONS, REASONS FOR CAUTION: The limited number of samples analysed in this study warrants external validation, as do the implications of our findings. Selection of male fertility patients was based on high basal levels of oxidative stress within their spermatozoa as opposed to specific sub-classes of male factor infertility. WIDER IMPLICATIONS OF THE FINDINGS: The identification of genomic regions susceptible to oxidation in the male germ line will be of value in focusing future analyses into the mutational load carried by children in response to paternal factors such as age, the treatment of male infertility using ART and paternal exposure to environmental toxicants. STUDY FUNDING/COMPETING INTEREST(S): Project support was provided by the University of Newcastle's (UoN) Priority Research Centre for Reproductive Science. M.J.X. was a recipient of a UoN International Postgraduate Research Scholarship. B.N. is the recipient of a National Health and Medical Research Council of Australia Senior Research Fellowship. Authors declare no conflict of interest.
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2019 |
Trigg NA, Eamens AL, Nixon B, 'The contribution of epididymosomes to the sperm small RNA profile.', Reproduction (Cambridge, England), 157 R209-R223 (2019) [C1]
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2018 |
Zhou W, De Iuliis GN, Dun MD, Nixon B, 'Characteristics of the epididymal luminal environment responsible for sperm maturation and storage', Frontiers in Endocrinology, 9 (2018) [C1]
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2018 |
Wang TE, Li SH, Minabe S, Andreson AL, Dun MD, Maeda KI, et al., 'Mouse quiescin sulfhydryl oxidases exhibit distinct epididymal luminal distribution with segment-specific sperm surface associations', Biology of Reproduction, 99 1022-1033 (2018) [C1]
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2018 |
Xavier MJ, Nixon B, Roman SD, Aitken RJ, 'Improved methods of DNA extraction from human spermatozoa that mitigate experimentally-induced oxidative DNA damage', PLOS ONE, 13 (2018) [C1]
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2018 |
Zhou W, Sipilä P, De Iuliis G, Dun MD, Nixon B, 'Analysis of Epididymal Protein Synthesis and Secretion', Jove-Journal of Visualized Experiments, 138 (2018) [C1]
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2018 |
Martin J, Bromfield EG, Aitken RJ, Lord T, Nixon B, 'Double Strand Break DNA Repair occurs via Non-Homologous End-Joining in Mouse MII Oocytes', Scientific Reports, 8 1-15 (2018) [C1]
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2018 |
Houston BJ, Nixon B, King BV, Aitken RJ, De Iuliis GN, 'Probing the Origins of 1,800 MHz Radio Frequency Electromagnetic Radiation Induced Damage in Mouse Immortalized Germ Cells and Spermatozoa in vitro', FRONTIERS IN PUBLIC HEALTH, 6 (2018) [C1]
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2018 |
Walters JLH, De Iuliis G, Nixon B, Bromfield EG, 'Oxidative Stress in the Male Germline: A Review of Novel Strategies to Reduce 4-Hydroxynonenal Production', Antioxidants, 7 (2018) [C1]
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2018 |
Walters JLH, De Iuliis GN, Dun MD, Aitken RJ, McLaughlin EA, Nixon B, Bromfield EG, 'Pharmacological inhibition of arachidonate 15-lipoxygenase protects human spermatozoa against oxidative stress.', Biology of reproduction, 98 784-794 (2018) [C1]
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2018 |
Mihalas BP, Bromfield EG, Sutherland JM, De Iuliis GN, McLaughlin EA, John Aitken R, Nixon B, 'Oxidative damage in naturally aged mouse oocytes is exacerbated by dysregulation of proteasomal activity', Journal of Biological Chemistry, 293 18944-18964 (2018) [C1]
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2018 |
Houston BJ, Nixon B, Martin JH, De Iuliis GN, Trigg NA, Bromfield EG, et al., 'Heat exposure induces oxidative stress and DNA damage in the male germ line', BIOLOGY OF REPRODUCTION, 98 593-606 (2018) [C1]
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2017 |
Zhou W, De Iuliis GN, Turner AP, Reid AT, Anderson AL, McCluskey A, et al., 'Developmental expression of the dynamin family of mechanoenzymes in the mouse epididymis', BIOLOGY OF REPRODUCTION, 96 159-173 (2017) [C1]
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2017 |
Bromfield EG, Aitken RJ, McLaughlin EA, Nixon B, 'Proteolytic degradation of heat shock protein A2 occurs in response to oxidative stress in male germ cells of the mouse', MOLECULAR HUMAN REPRODUCTION, 23 91-105 (2017) [C1]
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2017 |
Hall SE, Nixon B, Aitken RJ, 'Non-surgical sterilisation methods may offer a sustainable solution to feral horse (Equus caballus) overpopulation', Reproduction, Fertility and Development, 29 1655-1666 (2017) [C1]
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2017 |
Katen AL, Sipila P, Mitchell LA, Stanger SJ, Nixon B, Roman SD, 'Epididymal CYP2E1 plays a critical role in acrylamide-induced DNA damage in spermatozoa and paternally mediated embryonic resorptions', BIOLOGY OF REPRODUCTION, 96 921-935 (2017) [C1]
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2017 |
Bromfield EG, Mihalas BP, Dun MD, Aitken RJ, McLaughlin EA, Walters JLH, Nixon B, 'Inhibition of arachidonate 15-lipoxygenase prevents 4-hydroxynonenal-induced proteindamage in male germ cells', Biology of Reproduction, 96 598-609 (2017) [C1]
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2017 |
Johnston SD, Qualischefski E, Cooper J, McLeod R, Lever J, Nixon B, et al., 'Cryopreservation of saltwater crocodile (Crocodylus porosus) spermatozoa', Reproduction, Fertility and Development, 29 2235-2244 (2017) [C1]
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2017 |
Mihalas BP, De Iuliis GN, Redgrove KA, McLaughlin EA, Nixon B, 'The lipid peroxidation product 4-hydroxynonenal contributes to oxidative stress-mediated deterioration of the ageing oocyte', SCIENTIFIC REPORTS, 7 (2017) [C1]
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2017 |
Mihalas BP, Redgrove KA, McLaughlin EA, Nixon B, 'Molecular mechanisms responsible for increased vulnerability of the ageing oocyte to oxidative damage', Oxidative Medicine and Cellular Longevity, 2017 1-22 (2017) [C1]
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2017 |
Hutcheon K, McLaughlin EA, Stanger SJ, Bernstein IR, Dun MD, Eamens AL, Nixon B, 'Analysis of the small non-protein-coding RNA profile of mouse spermatozoa reveals specific enrichment of piRNAs within mature spermatozoa', RNA Biology, 14 1776-1790 (2017) [C1] © 2017 Taylor & Francis Group, LLC. Post-testicular sperm maturation and storage within the epididymis is a key determinant of gamete quality and fertilization competence. H... [more] © 2017 Taylor & Francis Group, LLC. Post-testicular sperm maturation and storage within the epididymis is a key determinant of gamete quality and fertilization competence. Here we demonstrate that mouse spermatozoa possess a complex small non-protein-coding RNA (sRNA) profile, the composition of which is markedly influenced by their epididymal transit. Thus, although microRNAs (miRNAs) are highly represented in the spermatozoa of the proximal epididymis, this sRNA class is largely diminished in mature spermatozoa of the distal epididymis. Coincident with this, a substantial enrichment in Piwi-interacting RNA (piRNA) abundance in cauda spermatozoa was detected. Further, features of cauda piRNAs, including; predominantly 29¿31 nts in length; preference for uracil at their 5' terminus; no adenine enrichment at piRNA nt 10, and; predominantly mapping to intergenic regions of the mouse genome, indicate that these piRNAs are generated by the PIWIL1-directed primary piRNA production pathway. Accordingly, PIWIL1 was detected via immunoblotting and mass spectrometry in epididymal spermatozoa. These data provide insight into the complexity and dynamic nature of the sRNA profile of spermatozoa and raise the intriguing prospect that piRNAs are generated in situ in maturing spermatozoa. Such information is of particular interest in view of the potential role for paternal sRNAs in influencing conception, embryo development and intergenerational inheritance.
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2017 |
Zhou W, Anderson AL, Turner AP, De Iuliis GN, McCluskey A, McLaughlin EA, Nixon B, 'Characterization of a novel role for the dynamin mechanoenzymes in the regulation of human sperm acrosomal exocytosis.', Molecular Human Reproduction, 23 657-673 (2017) [C1]
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2017 |
Martin JH, Bromfield EG, Aitken RJ, Nixon B, 'Biochemical alterations in the oocyte in support of early embryonic development', CELLULAR AND MOLECULAR LIFE SCIENCES, 74 469-485 (2017) [C1]
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2017 |
Hall SE, Aitken RJ, Nixon B, Smith ND, Gibb Z, 'Electrophilic aldehyde products of lipid peroxidation selectively adduct to heat shock protein 90 and arylsulfatase A in stallion spermatozoa', BIOLOGY OF REPRODUCTION, 96 107-121 (2017) [C1]
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2016 |
Redgrove KA, Bernstein IR, Pye VJ, Mihalas BP, Sutherland JM, Nixon B, et al., 'Dynamin 2 is essential for mammalian spermatogenesis', SCIENTIFIC REPORTS, 6 (2016) [C1]
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2016 |
Nixon B, Anderson AL, Smith ND, McLeod R, Johnston SD, 'The Australian saltwater crocodile (Crocodylus porosus) provides evidence that the capacitation of spermatozoa may extend beyond the mammalian lineage', Proceedings of the Royal Society B: Biological Sciences, 283 (2016) [C1] © 2016 The Author(s) Published by the Royal Society. All rights reserved. Although mammalian spermatozoa only acquire functional maturity as they are conveyed through the male (ep... [more] © 2016 The Author(s) Published by the Royal Society. All rights reserved. Although mammalian spermatozoa only acquire functional maturity as they are conveyed through the male (epididymal maturation) and female (capacitation) reproductive tracts, the degree of post-testicular development necessary to achieve fertilization in other vertebrate species remains far less clear. Indeed, despite reports that the epididymis of birds and reptiles is capable of secreting proteins that bind and modify the sperm surface characteristics, it remains unclear whether capacitation is a pre-requisite for fertilization in these species. Using the ancient reptilian Australian saltwater crocodile as a model, this study was undertaken to explore whether reptile sperm do undergo capacitation-like changes following ejaculation. Our studies revealed that crocodile spermatozoa experienced a rapid and sustained, cyclic-AMP mediated increase in progressive motility following incubation under conditions optimized for the induction of capacitation in mammalian species such as the mouse and human. This response was coupled with elevated levels of phosphorylation associated with both protein kinase A and tyrosine kinase substrates, the latter of which were predominantly localized within the sperm flagellum. In findings that also accord with mammalian spermatozoa, we confirmed a homologue of outer dense fibre 2 as one of the principal substrates for tyrosine phosphorylation. Overall, our findings support the concept that crocodile spermatozoa do undergo a process that is homologous to capacitation in preparation for fertilization of an ovum.
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2016 |
Houston B, Nixon B, King BV, De Iuliis GN, Aitken RJ, 'The effects of radiofrequency electromagnetic radiation on sperm function', Reproduction, 152 R263-R276 (2016) [C1]
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2016 |
Bromfield EG, McLaughlin EA, Aitken RJ, Nixon B, 'Heat shock protein member A2 forms a stable complex with angiotensin convertingenzymeand protein disulfide isomerase A6 in human spermatozoa', Molecular Human Reproduction, 22 93-109 (2016) [C1]
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2016 |
Martin JH, Nixon B, Lord T, Bromfield EG, Aitken RJ, 'Identification of a key role for permeability glycoprotein in enhancing the cellular defense mechanisms of fertilized oocytes', DEVELOPMENTAL BIOLOGY, 417 63-76 (2016) [C1]
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2016 |
Nixon B, Ecroyd H, Dacheux J-L, Dacheux F, Labas V, Johnston SD, Jones RC, 'Formation and Dissociation of Sperm Bundles in Monotremes', Biology of Reproduction, 95 1-11 (2016) [C1]
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2016 |
Stanger SJ, Law EA, Jamsai D, O'Bryan MK, Nixon B, McLaughlin EA, et al., 'A novel germ cell protein, SPIF (sperm PKA interacting factor), is essential for the formation of a PKA/TCP11 complex that undergoes conformational and phosphorylation changes upon capacitation', FASEB Journal, 30 2777-2791 (2016) [C1] © 2016 FASEB. Spermatozoa require the process of capacitation to enable them to fertilize an egg. PKA is crucial to capacitation and the development of hyperactivated motility. Sp... [more] © 2016 FASEB. Spermatozoa require the process of capacitation to enable them to fertilize an egg. PKA is crucial to capacitation and the development of hyperactivated motility. Sperm PKA is activated by cAMP generated by the germ cell-enriched adenylyl cyclase encoded by Adcy10. Male mice lacking Adcy10 are sterile, because their spermatozoa are immotile. The current studywas designed to identify binding partners of the sperm-specific (Ca2) catalytic subunit of PKA (PRKACA) by using it as the "bait" in a yeast 2-hybrid system. This approach was used to identify a novel germ cell-enriched protein, sperm PKA interacting factor (SPIF), in 25% of the positive clones. Homozygous Spif-nullmice were embryonically lethal. SPIF was coexpressed and coregulated with PRKACA and with t-complex protein (TCP)-11, a protein associated with PKA signaling. We established that these 3 proteins form part of a novel complex in mouse spermatozoa. Upon capacitation, the SPIF protein becomes tyrosine phosphorylatedin > 95% of sperm. Anapparent molecular rearrangement in the complex occurs, bringing PRKACA and TCP11 into proximity. Taken together, these results suggest a role for the novel complex of SPIF, PRKACA, and TCP11 during spermcapacitation, fertilization, and embryogenesis.
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2016 |
Aitken RJ, Muscio L, Whiting S, Connaughton HS, Fraser BA, Nixon B, et al., 'Analysis of the effects of polyphenols on human spermatozoa reveals unexpected impacts on mitochondrial membrane potential, oxidative stress and DNA integrity; implications for assisted reproductive technology', Biochemical Pharmacology, 121 78-96 (2016) [C1]
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2016 |
Reilly JN, McLaughlin EA, Stanger SJ, Anderson AL, Hutcheon K, Church K, et al., 'Characterisation of mouse epididymosomes reveals a complex profile of microRNAs and a potential mechanism for modification of the sperm epigenome', SCIENTIFIC REPORTS, 6 (2016) [C1]
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2016 |
Katen AL, Chambers CG, Nixon B, Roman SD, 'Chronic Acrylamide Exposure in Male Mice Results in Elevated DNA Damage in the Germline and Heritable Induction of CYP2E1 in the Testes', BIOLOGY OF REPRODUCTION, 95 (2016) [C1]
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2016 |
Katen AL, Stanger SJ, Anderson AL, Nixon B, Roman SD, 'Chronic acrylamide exposure in male mice induces DNA damage to spermatozoa; Potential for amelioration by resveratrol', REPRODUCTIVE TOXICOLOGY, 63 1-12 (2016) [C1]
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2015 |
Anderson AL, Stanger SJ, Mihalas BP, Tyagi S, Holt JE, McLaughlin EA, Nixon B, 'Assessment of microRNA expression in mouse epididymal epithelial cells and spermatozoa by next generation sequencing', Genomics Data, 6 208-211 (2015) [C1] © 2015 Elsevier Inc. The mammalian epididymis is a highly specialized region of the male reproductive tract that is lined with a continuous layer of epithelial cells that display ... [more] © 2015 Elsevier Inc. The mammalian epididymis is a highly specialized region of the male reproductive tract that is lined with a continuous layer of epithelial cells that display a remarkable level of regionalized secretory and absorptive activity. The luminal environment created by this combined secretory and absorptive activity is directly responsible for promoting the functional maturation of spermatozoa and their maintenance in a quiescent and viable state prior to ejaculation. This study was designed to identify the complement of microRNAs (miRNAs) that are expressed within the mouse epididymal epithelial cells and the maturing populations of spermatozoa. Through the use of Next Generation Sequencing technology we have demonstrated that both epididymal epithelial cells and spermatozoa harbour a complex repertoire of miRNAs that have substantially different expression profiles along the length of the tract. These data, deposited in the Gene Expression Omnibus (GEO) with the accession numbers GSE70197 and GSE70198, afford valuable insight into the post-transcriptional control of gene expression within the epididymis and provide the first evidence for the dynamic transformation of the miRNA content of maturing sperm cells. Ultimately such information promises to inform our understanding of the aetiology of male infertility. Herein we provide a detailed description of the methodology used to generate these important data.
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2015 |
Bromfield E, Aitken RJ, Nixon B, 'Novel characterization of the HSPA2-stabilizing protein BAG6 in human spermatozoa', Molecular Human Reproduction, 21 755-769 (2015) [C1] © The Author 2015. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. While a large cohort of sperm ... [more] © The Author 2015. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. While a large cohort of sperm surface receptors underpin sperm-oocyte adhesion processes, our recent work has revealed that the molecular chaperone Heat Shock Protein A2 (HSPA2) is a key regulator of zona pellucida-receptor complex assembly in our own species. Indeed, in the infertile population, spermatozoa that fail to interact with the zona pellucida of the oocyte consistently lack HSPA2 protein expression. While the mechanisms behind this protein deficiency are under consideration, BCL2-associated athanogene 6 (BAG6) has been identified as a key regulator of HSPA2 stability in mouse germ cells. However, in the human, the presence of BAG family proteins remains completely uncharacterized. Consequently, this study aimed to determine the presence of BAG6 in human sperm cells and to characterize its putative interaction with HSPA2 throughout sperm cell development. BAG6 was shown to co-localize with HSPA2 in human testicular germ cells and epididymal spermatozoa. Similarly, BAG6 was identified in the equatorial region of non-capacitated spermatozoa but underwent a marked relocation to the anterior region of the head upon the induction of capacitation in these cells. Protein-protein interaction assays revealed the stable interaction of BAG6 and HSPA2 proteins in mature spermatozoa. Furthermore, examination of the spermatozoa of infertile men with zona pellucida binding defects, related to a lack of HSPA2, revealed a concomitant deficiency in BAG6 protein expression. In view of the findings described in this study, we propose that BAG6 is likely a key regulator of HSPA2 stability/function in human germ cells. Moreover, its under-representation in spermatozoa with zona pellucida binding deficiency suggests that BAG6 may be an important candidate to study for a further understanding of male idiopathic infertility.
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2015 |
Nixon B, Stanger SJ, Mihalas BP, Reilly JN, Anderson AL, Dun MD, et al., 'Next generation sequencing analysis reveals segmental patterns of microRNA expression in mouse epididymal epithelial cells', PLoS ONE, 10 (2015) [C1] © 2015 Nixon et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and repro... [more] © 2015 Nixon et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. The functional maturation of mammalian spermatozoa is accomplished as the cells descend through the highly specialized microenvironment of the epididymis. This dynamic environment is, in turn, created by the combined secretory and absorptive activity of the surrounding epithelium and displays an extraordinary level of regionalization. Although the regulatory network responsible for spatial coordination of epididymal function remains unclear, recent evidence has highlighted a novel role for the RNA interference pathway. Indeed, as noncanonical regulators of gene expression, small noncoding RNAs have emerged as key elements of the circuitry involved in regulating epididymal function and hence sperm maturation. Herein we have employed next generation sequencing technology to profile the genome-wide miRNA signatures of mouse epididymal cells and characterize segmental patterns of expression. An impressive profile of some 370 miRNAs were detected in the mouse epididymis, with a subset of these specifically identified within the epithelial cells that line the tubule (218). A majority of the latter miRNAs (75%) were detected at equivalent levels along the entire length of the mouse epididymis. We did however identify a small cohort of miRNAs that displayed highly regionalized patterns of expression, including miR-204-5p and miR-196b-5p, which were down- and up-regulated by approximately 39- and 45-fold between the caput/caudal regions, respectively. In addition we identified 79 miRNAs (representing ~ 21% of all miRNAs) as displaying conserved expression within all regions of the mouse, rat and human epididymal tissue. These included 8/14 members of let-7 family of miRNAs that have been widely implicated in the control of androgen signaling and the repression of cell proliferation and oncogenic pathways. Overall these data provide novel insights into the sophistication of the miRNA network that regulates the function of the male reproductive tract.
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2015 |
Aitken RJ, Cummins JM, Nixon B, 'The 12 (th) International Symposium on Spermatology.', Asian J Androl, 17 519-520 (2015) [C3]
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2015 |
Aitken RJ, Baker MA, Nixon B, 'Are sperm capacitation and apoptosis the opposite ends of a continuum driven by oxidative stress?', Asian J Androl, 17 633-639 (2015) [C1]
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2015 |
Nixon B, Stanger SJ, Mihalas BP, Reilly JN, Anderson AL, Tyagi S, et al., 'The MicroRNA Signature of Mouse Spermatozoa Is Substantially Modified During Epididymal Maturation', Biology of Reproduction, 93 (2015) [C1] © 2015 by the Society for the Study of Reproduction, Inc. In recent years considerable effort has been devoted to understanding the epigenetic control of sperm development, leadin... [more] © 2015 by the Society for the Study of Reproduction, Inc. In recent years considerable effort has been devoted to understanding the epigenetic control of sperm development, leading to an increased appreciation of the importance of RNA interference pathways, and in particular miRNAs, as key regulators of spermatogenesis and epididymal maturation. It has also been shown that sperm are endowed with an impressive array of miRNA that have been implicated in various aspects of fertilization and embryo development. However, to date there have been no reports on whether the sperm miRNA signature is static or whether it is influenced by their prolonged maturation within the male reproductive tract. To investigate this phenomenon, we employed next-generation sequencing to systematically profile the miRNA signature of maturing mouse spermatozoa. In so doing we have provided the first evidence for the posttesticular modification of the sperm miRNA profile under normal physiological conditions. Such modifications include the apparent loss and acquisition of an impressive cohort of some 113 and 115 miRNAs, respectively, between the proximal and distal epididymal segments. Interestingly, the majority of these changes occur late in maturation and include the uptake of novel miRNA species in addition to a significant increase in many miRNAs natively expressed in immature sperm. Because sperm are not capable of de novo transcription, these findings identify the epididymis as an important site in establishing the sperm epigenome with the potential to influence the peri-conceptual environment of the female reproductive tract, contribute to the inheritance of acquired characteristics, and/or alter the developmental trajectory of the resulting offspring.
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2015 |
Reid AT, Anderson AL, Roman SD, McLaughlin EA, McCluskey A, Robinson PJ, et al., 'Glycogen synthase kinase 3 regulates acrosomal exocytosis in mouse spermatozoa via dynamin phosphorylation', FASEB JOURNAL, 29 2872-2882 (2015) [C1]
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2015 |
Bromfield EG, Aitken RJ, Anderson AL, McLaughlin EA, Nixon B, 'The impact of oxidative stress on chaperone-mediated human sperm-egg interaction.', Hum Reprod, 30 2597-2613 (2015) [C1]
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2015 |
Nixon B, Bromfield EG, Dun MD, Redgrove KA, McLaughlin EA, Aitken RJ, 'The role of the molecular chaperone heat shock protein A2 (HSPA2) in regulating human sperm-egg recognition', ASIAN JOURNAL OF ANDROLOGY, 17 568-573 (2015) [C1]
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2014 |
Nixon BJ, Katen AL, Stanger SJ, Schjenken JE, Nixon B, Roman SD, 'Mouse spermatocytes express CYP2E1 and respond to acrylamide exposure', PLoS ONE, 9 (2014) [C1] Metabolism of xenobiotics by cytochrome P450s (encoded by the CYP genes) often leads to bio-activation, producing reactive metabolites that interfere with cellular processes and c... [more] Metabolism of xenobiotics by cytochrome P450s (encoded by the CYP genes) often leads to bio-activation, producing reactive metabolites that interfere with cellular processes and cause DNA damage. In the testes, DNA damage induced by xenobiotics has been associated with impaired spermatogenesis and adverse effects on reproductive health. We previously reported that chronic exposure to the reproductive toxicant, acrylamide, produced high levels of DNA damage in spermatocytes of Swiss mice. CYP2E1 metabolises acrylamide to glycidamide, which, unlike acrylamide, readily forms adducts with DNA. Thus, to investigate the mechanisms of acrylamide toxicity in mouse male germ cells, we examined the expression of the CYP, CYP2E1, which metabolises acrylamide. Using Q-PCR and immunohistochemistry, we establish that CYP2E1 is expressed in germ cells, in particular in spermatocytes. Additionally, CYP2E1 gene expression was upregulated in these cells following in vitro acrylamide exposure (1 µM, 18 h). Spermatocytes were isolated and treated with 1 µM acrylamide or 0.5 µM glycidamide for 18 hours and the presence of DNA-adducts was investigated using the comet assay, modified to detect DNA-adducts. Both compounds produced significant levels of DNA damage in spermatocytes, with a greater response observed following glycidamide exposure. A modified comet assay indicated that direct adduction of DNA by glycidamide was a major source of DNA damage. Oxidative stress played a small role in eliciting this damage, as a relatively modest effect was found in a comet assay modified to detect oxidative adducts following glycidamide exposure, and glutathione levels remained unchanged following treatment with either compound. Our results indicate that the male germ line has the capacity to respond to xenobiotic exposure by inducing detoxifying enzymes, and the DNA damage elicited by acrylamide in male germ cells is likely due to the formation of glycidamide adducts. © 2014 Nixon et al.
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2014 |
Bromfield EG, Aitken RJ, Gibb Z, Lambourne SR, Nixon B, 'Capacitation in the presence of methyl-beta-cyclodextrin results in enhanced zona pellucida-binding ability of stallion spermatozoa', REPRODUCTION, 147 153-166 (2014) [C1]
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2014 |
Nixon B, Ewen KA, Krivanek KM, Clulow J, Kidd G, Ecroyd H, Jones RC, 'Post-testicular sperm maturation and identification of an epididymal protein in the Japanese quail (Coturnix coturnix japonica)', REPRODUCTION, 147 265-277 (2014) [C1]
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2013 |
McIver SC, Roman SD, Nixon B, Loveland KL, McLaughlin EA, 'The rise of testicular germ cell tumours: The search for causes, risk factors and novel therapeutic targets', F1000 Research, 2 1-11 (2013) [C1]
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2013 |
Lord T, Nixon B, Jones KT, Aitken RJ, 'Melatonin Prevents Postovulatory Oocyte Aging in the Mouse and Extends the Window for Optimal Fertilization In Vitro', Biology of Reproduction, 88 1-9 (2013) [C1]
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2013 |
Nixon BJ, Stanger SJ, Nixon B, Roman SD, 'Erratum to Chronic Exposure to Acrylamide Induces DNA Damage in Male Germ Cells of Mice [Toxicological Sciences, 129, 1 (2012) 135-145] doi:10.1093/toxsci/kfs178', Toxicological Sciences, 132 250 (2013)
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2013 |
Redgrove KA, Anderson AL, McLaughlin EA, O'Bryan MK, Aitken RJ, Nixon B, 'Investigation of the mechanisms by which the molecular chaperone HSPA2 regulates the expression of sperm surface receptors involved in human spermoocyte recognition', MOLECULAR HUMAN REPRODUCTION, 19 120-135 (2013) [C1]
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2013 |
Aitken RJ, Nixon B, 'Sperm capacitation: a distant landscape glimpsed but unexplored', Molecular Human Reproduction, 19 785-793 (2013) [C1]
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2013 |
Bromfield EG, Nixon B, 'The function of chaperone proteins in the assemblage of protein complexes involved in gamete adhesion and fusion processes', Reproduction, 145 R31-R42 (2013) [C1]
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2013 |
McIver SC, Loveland KL, Roman SD, Nixon B, Kitazawa R, McLaughlin EA, 'The chemokine CXCL12 and its receptor CXCR4 are implicated in human seminoma metastasis', ANDROLOGY, 1 517-529 (2013) [C1]
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2012 |
Reid AT, Lord T, Stanger SJ, Roman SD, McCluskey A, Robinson PJ, et al., 'Dynamin regulates specific membrane fusion events necessary for acrosomal exocytosis in mouse spermatozoa', Journal of Biological Chemistry, 287 37659-37672 (2012) [C1]
|
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2012 |
Sutherland JM, Keightley RA, Nixon B, Roman SD, Robker RL, Russell DL, McLaughlin EA, 'Suppressor of cytokine signaling 4 (SOCS4): Moderator of ovarian primordial follicle activation', Journal of Cellular Physiology, 227 1188-1198 (2012) [C1]
|
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2012 |
Sobinoff AP, Pye VJ, Nixon B, Roman SD, McLaughlin EA, 'Jumping the gun: Smoking constituent BaP causes premature primordial follicle activation and impairs oocyte fusibility through oxidative stress', Toxicology and Applied Pharmacology, 260 70-80 (2012) [C1]
|
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2012 |
Dun MD, Anderson AL, Bromfield EG, Asquith KL, Emmett BJ, McLaughlin EA, et al., 'Investigation of the expression and functional significance of the novel mouse sperm protein, a disintegrin and metalloprotease with thrombospondin type 1 motifs number 10 (ADAMTS10)', International Journal of Andrology, 35 572-589 (2012) [C1]
|
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2012 |
Sobinoff AP, Nixon B, Roman SD, McLaughlin EA, 'Staying alive: PI3K pathway promotes primordial follicle activation and survival in response to 3MC-induced ovotoxicity', Toxicological Sciences, 128 258-271 (2012) [C1]
|
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2012 |
Nixon BJ, Stanger SJ, Nixon B, Roman SD, 'Chronic exposure to acrylamide induces DNA damage in male germ cells of mice', Toxicological Sciences, 129 135-145 (2012) [C1]
|
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2012 |
McIver SC, Roman SD, Nixon B, McLaughlin EA, 'miRNA and mammalian male germ cells', Human Reproduction Update, 18 44-59 (2012) [C1]
|
||||||||||
2012 |
Dun MD, Aitken RJ, Nixon B, 'The role of molecular chaperones in spermatogenesis and the post-testicular maturation of mammalian spermatozoa', Human Reproduction Update, 18 420-435 (2012) [C1]
|
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2012 |
McIver SC, Stanger SJ, Santarelli DMF, Roman SD, Nixon B, McLaughlin EA, 'A unique combination of male germ cell mirnas coordinates gonocyte differentiation', PLoS One, 7 1-14 (2012) [C1]
|
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2012 |
Redgrove KA, Nixon B, Baker MA, Hetherington L, Baker G, Liu D-Y, Aitken RJ, 'The molecular chaperone HSPA2 plays a key role in regulating the expression of sperm surface receptors that mediate sperm-egg recognition', Plos One, 7 1-16 (2012) [C1]
|
||||||||||
2012 |
Baker MA, Nixon B, Naumovski N, Aitken RJ, 'Proteomic insights into the maturation and capacitation of mammalian spermatozoa', Systems Biology in Reproductive Medicine, 58 211-217 (2012) [C1]
|
||||||||||
2011 |
Redgrove KA, Anderson AL, Dun MD, McLaughlin EA, O'Bryan MK, Aitken RJ, Nixon B, 'Involvement of multimeric protein complexes in mediating the capacitation-dependent binding of human spermatozoa to homologous zonae pellucidae', Developmental Biology, 356 460-474 (2011) [C1]
|
||||||||||
2011 |
Dun MD, Smith ND, Baker MA, Lin M, Aitken RJ, Nixon B, 'The chaperonin containing TCP1 complex (CCT/TRiC) is involved in mediating sperm-oocyte interaction', Journal of Biological Chemistry, 286 36875-36887 (2011) [C1]
|
||||||||||
2011 |
Nixon B, Mitchell LA, Anderson AL, McLaughlin EA, O'Bryan MK, Aitken RJ, 'Proteomic and functional analysis of human sperm detergent resistant membranes', Journal of Cellular Physiology, 226 2651-2665 (2011) [C1]
|
||||||||||
2011 |
Ainsworth CJ, Nixon B, Aitken RJ, 'The electrophoretic separation of spermatozoa: An analysis of genotype, surface carbohydrate composition and potential for capacitation', International Journal of Andrology, 34 e422-e434 (2011) [C1]
|
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2011 |
Nixon B, Ecroyd HW, Dacheux J-L, Jones RC, 'Monotremes provide a key to understanding the evolutionary significance of epididymal sperm maturation', Journal of Andrology, 32 665-671 (2011) [C1]
|
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2011 |
Sobinoff AP, Mahony M, Nixon B, Roman SD, McLaughlin EA, 'Understanding the villain: DMBA-induced preantral ovotoxicity involves selective follicular destruction and primordial follicle activation through PI3K/Akt and mTOR signaling', Toxicological Sciences, 123 563-575 (2011) [C1]
|
||||||||||
2010 |
Gibbs GM, Lo JCY, Nixon B, Jamsai D, O'Connor AE, Rijal S, et al., 'Glioma pathogenesis-related 1-Like 1 is testis enriched, dynamically modified, and redistributed during male germ cell maturation and has a potential role in sperm-oocyte binding', Endocrinology, 151 2331-2342 (2010) [C1]
|
||||||||||
2010 |
Reid AT, Redgrove KA, Aitken RJ, Nixon B, 'Cellular mechanisms regulating sperm-zona pellucida interaction', Asian Journal of Andrology, 13 88-96 (2010) [C1]
|
||||||||||
2010 |
Sobinoff AP, Pye VJ, Nixon B, Roman SD, McLaughlin EA, 'Adding insult to injury: Effects of xenobiotic-induced preantral ovotoxicity on ovarian development and oocyte fusibility', Toxicological Sciences, 118 653-666 (2010) [C1]
|
||||||||||
2010 |
Nixon B, Bielanowicz AJ, Anderson AL, Walsh AK, Hall TE, McCloghry AK, Aitken RJ, 'Elucidation of the signaling pathways that underpin capacitation-associated surface phosphotyrosine expression in mouse spermatozoa', Journal of Cellular Physiology, 224 71-83 (2010) [C1]
|
||||||||||
2009 |
Bennetts LE, De Iuliis GN, Nixon B, Kime M, Zelski K, McVicar CM, et al., 'Impact of Estrogenic Compounds on DNA Integrity in Human Spermatozoa: Evidence for Cross-Linking and Redox Cycling Activities', JOURNAL OF UROLOGY, 181 914-915 (2009) [C1]
|
||||||||||
2009 |
De Iuliis GN, Thomson LK, Mitchell LA, Read JM, Koppers AJ, Hedges A, et al., 'DNA damage in human spermatozoa is highly correlated with the efficiency of chromatin remodeling and the formation of 8-hydroxy-2'-deoxyguanosine, a marker of oxidative stress', Biology of Reproduction, 81 517-524 (2009) [C1]
|
||||||||||
2009 |
Nixon B, Bielanowicz AJ, McLaughlin EA, Tanphaichitr N, Ensslin MA, Aitken RJ, 'Composition and significance of detergent resistant membranes in mouse spermatozoa', Journal of Cellular Physiology, 218 122-134 (2009) [C1]
|
||||||||||
2009 |
Nixon B, Aitken RJ, 'The biological significance of detergent-resistant membranes in spermatozoa', Journal of Reproductive Immunology, 83 8-13 (2009) [C1]
|
||||||||||
2009 |
Dacheux J-L, Dacheux F, Labas V, Ecroyd H, Nixon B, Jones RC, 'New proteins identified in epididymal fluid from the platypus (Ornithorhynchus anatinus)', Reproduction Fertility and Development, 21 1002-1007 (2009) [C1]
|
||||||||||
2009 |
Ecroyd H, Nixon B, Dacheux J-L, Jones RC, 'Testicular descent, sperm maturation and capacitation: Lessons from our most distant relatives, the monotremes', Reproduction Fertility and Development, 21 992-1001 (2009) [C1]
|
||||||||||
2008 |
Mitchell LA, Nixon B, Baker MA, Aitken RJ, 'Investigation of the role of SRC in capacitation-associated tyrosine phosphorylation of human spermatozoa', Molecular Human Reproduction, 14 235-243 (2008) [C1]
|
||||||||||
2008 |
Grutzner F, Nixon B, Jones RC, 'Reproductive biology in egg-laying mammals', Sexual Development, 2 115-127 (2008) [C1]
|
||||||||||
2008 |
Nixon B, Jones RC, Holland MK, 'Molecular and functional characterization of the rabbit epididymal secretory protein 52, REP52', Biology of Reproduction, 78 910-920 (2008) [C1]
|
||||||||||
2008 |
Walsh AK, Whelan D, Bielanowicz AJ, Skinner BL, Aitken RJ, O'Bryan MK, Nixon B, 'Identification of the molecular chaperone, heat shock protein 1 (chaperonin 10), in the reproductive tract and in capacitating spermatozoa in the male mouse', Biology of Reproduction, 78 983-993 (2008) [C1]
|
||||||||||
2008 |
Warren WC, Hillier LW, Graves JAM, Birney E, Ponting CP, Grutzner F, et al., 'Genome analysis of the platypus reveals unique signatures of evolution', Nature, 453 175-U1 (2008) [C1]
|
||||||||||
2008 |
Priestley MN, Dunstan RH, O'Connor WA, Van Zwieten L, Nixon B, Macfarlane GR, 'Effects of 4-nonylphenol and 17 alpha-ethynylestradiol exposure in the Sydney rock oyster, Saccostrea glomerata: Vitellogenin induction and gonadal development', Aquatic Toxicology, 88 39-47 (2008) [C1]
|
||||||||||
2007 |
Aitken RJ, Nixon B, Lin M, Koppers AJ, Lee YH, Baker MA, 'Proteomic changes in mammalian spermatozoa during epididymal maturation', Asian Journal of Andrology, 9 554-564 (2007) [C1]
|
||||||||||
2007 |
Jones RC, Dacheux JL, Nixon B, Ecroyd HW, 'Role of the epididymis in sperm competition', Asian Journal of Andrology, 9 493-499 (2007) [C1]
|
||||||||||
2007 |
Mitchell LA, Nixon B, Aitken RJ, 'Analysis of chaperone proteins associated with human spermatozoa during capacitation', Molecular Human Reproduction, 13 605-613 (2007) [C1]
|
||||||||||
2007 |
Nixon B, Aitken RJ, McLaughlin EA, 'New insights into the molecular mechanisms of sperm-egg interaction', Cellular and Molecular Life Sciences, 64 1805-1823 (2007) [C1]
|
||||||||||
2007 |
Ainsworth CJ, Nixon B, Jansen RPS, Aitken RJ, 'First recorded pregnancy and normal birth after ICSI using electrophoretically isolated spermatozoa', Human Reproduction, 22 197-200 (2007) [C1]
|
||||||||||
2006 |
Nixon B, Macintyre DA, Mitchell LA, Gibbs GM, O'Bryan M, Aitken RJ, 'The identification of mouse sperm-surface-associated proteins and characterization of their ability to act as decapacitation factors', Biology of Reproduction, 74 275-287 (2006) [C1]
|
||||||||||
2005 |
Nixon B, Paul JW, Spiller CM, Attwell-Heap AG, Ashman LK, Aitken RJ, 'Evidence for the involvement of PECAM-1 in a receptor mediated signal-transduction pathway regulating capacitation-associated tyrosine phosphorylation in human spermatozoa', Journal of Cell Science, 118 4865-4877 (2005) [C1]
|
||||||||||
2005 |
Nixon B, Asquith KL, Aitken RJ, 'The role of molecular chaperones in mouse sperm-egg interactions', Molecular and Cellular Endocrinology, 240 1-10 (2005) [C1]
|
||||||||||
2005 |
Ainsworth CJ, Nixon B, Aitken RJ, 'Development of a novel electrophoretic system for the isolation of human spermatozoa', Human Reproduction, 20 2261-2270 (2005) [C1]
|
||||||||||
2005 |
Asquith KL, Bielanowicz AJ, McLaughlin EA, Nixon B, Aitken RJ, 'Localization and significance of molecular chaperones, heat shock protein 1, and tumor rejection antigen gp96 in the male reproductive tract and during capacitation and acrosome reaction', Biology of Reproduction, 72 328-337 (2005) [C1]
|
||||||||||
2004 |
Asquith KL, Baleato R, McLaughlin EA, Nixon B, Aitken RJ, 'Tyrosine phosphorylation activates surface chaperones facilitating sperm-zona recognition', Journal of Cell Science, 117 3645-3657 (2004) [C1]
|
||||||||||
2004 |
Curry BJ, Su H, Law EG, McLaughlin EA, Nixon B, Aitken RJ, 'Identification of RARhoGAP, a novel putative RhoGAP gene expressed in male germ cells', Genomics, 84 406-418 (2004) [C1]
|
||||||||||
2002 |
Nixon B, Jones RC, Hansen LA, Holland M, 'Rabbit epididymal secretory proteins. I. Characterization and hormonal regulation', Biology of Reproduction, 67 133-139 (2002) [C1]
|
||||||||||
2002 |
Nixon B, Jones RC, Clarke H, Holland M, 'Rabbit epididymal secretory proteins. II. Immunolocalization and sperm association of REP38', Biology of Reproduction, 67 140-146 (2002) [C1]
|
||||||||||
2002 |
Nixon B, Hardy C, Jones RC, Anfrews J, Holland M, 'Rabbit epididymal secretory proteins. III. Molecular cloning and characterization of the complementary DNA for REP38', Biology of Reproduction, 67 147-153 (2002) [C1]
|
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2001 |
Nixon B, Lu QX, Wassler MJ, Foote CI, Ensslin MA, Shur BD, 'Galactosyltransferase function during mammalian fertilization', CELLS TISSUES ORGANS, 168 46-57 (2001) [C1]
|
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Show 139 more journal articles |
Review (2 outputs)
Year | Citation | Altmetrics | Link | ||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
2013 |
McIver SM, Roman SD, Nixon B, Loveland KL, McLaughlin EA, 'The rise of testicular germ cell tumours: the search for causes, risk factors and novel therapeutic targets', F1000Research (2013)
|
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2007 |
Nixon B, Aitken RJ, McLaughlin EA, 'New insights into the molecular mechanisms of sperm-egg interaction', CELLULAR AND MOLECULAR LIFE SCIENCES (2007) [D1]
|
Conference (1 outputs)
Year | Citation | Altmetrics | Link | ||
---|---|---|---|---|---|
2020 |
Duchatel RJ, Jackson ER, Mannan A, Staudt D, Skerrett-Byrne DA, Nixon B, et al., 'Targeting phosphatidylinositol-4,5-bisphosphate 3-kinase (PI3K) and protein kinase C (PKC) activation in diffuse midline glioma (DMG)', ASIA-PACIFIC JOURNAL OF CLINICAL ONCOLOGY (2020)
|
Grants and Funding
Summary
Number of grants | 66 |
---|---|
Total funding | $14,307,807 |
Click on a grant title below to expand the full details for that specific grant.
20212 grants / $484,694
2021 HMRI MRSP - Pregnancy and Reproduction Program$325,846
Funding body: Hunter Medical Research Institute
Funding body | Hunter Medical Research Institute |
---|---|
Project Team | Laureate Professor Roger Smith, Laureate Professor John Aitken, Professor Craig Pennell, Professor Brett Nixon, Associate Professor Kirsty Pringle, Associate Professor Mark Baker, Professor Jon Hirst, Doctor Elizabeth Bromfield, Doctor Jonathan Paul, Doctor Geoffry De Iuliis, Doctor Yogavijayan Kandasamy, Doctor Jessie Sutherland, Professor Lee Smith |
Scheme | NSW MRSP Infrastructure Grant |
Role | Investigator |
Funding Start | 2021 |
Funding Finish | 2021 |
GNo | G2001363 |
Type Of Funding | C2220 - Aust StateTerritoryLocal - Other |
Category | 2220 |
UON | Y |
The Diagnosis and Resolution of Oxidative Stress in the Male Germ Line$158,848
Funding body: Memphasys
Funding body | Memphasys |
---|---|
Project Team | Laureate Professor John Aitken, Doctor Hassan Bakos, Professor Brett Nixon, Alison Coutts, Miss Alena Hungerford |
Scheme | PhD Scholarship |
Role | Investigator |
Funding Start | 2021 |
Funding Finish | 2024 |
GNo | G2001516 |
Type Of Funding | C3111 - Aust For profit |
Category | 3111 |
UON | Y |
20203 grants / $2,312,686
Utilising male fertility as a biomarker of health to understand the biological effects of PFAS$1,319,338
Funding body: NHMRC (National Health & Medical Research Council)
Funding body | NHMRC (National Health & Medical Research Council) |
---|---|
Project Team | Professor Brett Nixon, Associate Professor Brett Turner, Associate Professor Brett Turner, Doctor Geoffry De Iuliis, Doctor Geoffry De Iuliis, Dr Bradley Clarke, Doctor Shaun Roman, Doctor Shaun Roman, Associate Professor Matt Dun, Associate Professor Matt Dun, Doctor Andy Eamens, Doctor Andy Eamens, Dr Mark Green, Dr Mark Green, Dr Bradley Clarke |
Scheme | Targeted Call for Research - Per and Poly-Fluoroalkylated Substances (PFAS) |
Role | Lead |
Funding Start | 2020 |
Funding Finish | 2023 |
GNo | G1900626 |
Type Of Funding | C1100 - Aust Competitive - NHMRC |
Category | 1100 |
UON | Y |
Investigating the role of hypoxic niche microenvironments and hypoxia-induced transcription factors in regulating spermatogonial stem cell function$567,590
Funding body: NHMRC (National Health & Medical Research Council)
Funding body | NHMRC (National Health & Medical Research Council) |
---|---|
Project Team | Doctor Tessa Lord, Professor Brett Nixon |
Scheme | Ideas Grants |
Role | Investigator |
Funding Start | 2020 |
Funding Finish | 2022 |
GNo | G1900404 |
Type Of Funding | Aust Competitive - Commonwealth |
Category | 1CS |
UON | Y |
MRSP 2020 Pregnancy and Reproduction Program$425,758
Funding body: Hunter Medical Research Institute
Funding body | Hunter Medical Research Institute |
---|---|
Project Team | Laureate Professor Roger Smith, Laureate Professor John Aitken, Professor Craig Pennell, Professor Lee Smith, Professor Jon Hirst, Professor Brett Nixon, Associate Professor Kirsty Pringle, Doctor Jonathan Paul, Doctor Jessie Sutherland |
Scheme | NSW MRSP Infrastructure Grant |
Role | Investigator |
Funding Start | 2020 |
Funding Finish | 2021 |
GNo | G1901541 |
Type Of Funding | C2220 - Aust StateTerritoryLocal - Other |
Category | 2220 |
UON | Y |
20194 grants / $1,701,739
Mechanistic and translational studies in male reproductive health$666,292
Funding body: NHMRC (National Health & Medical Research Council)
Funding body | NHMRC (National Health & Medical Research Council) |
---|---|
Project Team | Professor Brett Nixon |
Scheme | Research Fellowships |
Role | Lead |
Funding Start | 2019 |
Funding Finish | 2023 |
GNo | G1800012 |
Type Of Funding | Aust Competitive - Commonwealth |
Category | 1CS |
UON | Y |
Targeted disruption of lipoxygenase enzymes to prevent oxidative stress-mediated pathologies in the male germline$586,586
Funding body: NHMRC (National Health & Medical Research Council)
Funding body | NHMRC (National Health & Medical Research Council) |
---|---|
Project Team | Professor Brett Nixon, Doctor Elizabeth Bromfield, Laureate Professor John Aitken |
Scheme | Project Grant |
Role | Lead |
Funding Start | 2019 |
Funding Finish | 2021 |
GNo | G1800389 |
Type Of Funding | Aust Competitive - Commonwealth |
Category | 1CS |
UON | Y |
Utilising the male germline to define our understanding of the biological effects of radio frequency electromagnetic energy$435,307
Funding body: NHMRC (National Health & Medical Research Council)
Funding body | NHMRC (National Health & Medical Research Council) |
---|---|
Project Team | Doctor Geoffry De Iuliis, Dr King Yuk Chan, chan, King Yuk, Professor Brett Nixon, Professor Rodica Ramer, Ramer, Rodica |
Scheme | Project Grant |
Role | Investigator |
Funding Start | 2019 |
Funding Finish | 2021 |
GNo | G1800050 |
Type Of Funding | Aust Competitive - Commonwealth |
Category | 1CS |
UON | Y |
Research Consultancy$13,554
Funding body: Kazia Laboratories Pty Limited
Funding body | Kazia Laboratories Pty Limited |
---|---|
Project Team | Doctor Elizabeth Bromfield, Professor Brett Nixon |
Scheme | Research Consultancy |
Role | Investigator |
Funding Start | 2019 |
Funding Finish | 2019 |
GNo | G1900374 |
Type Of Funding | C3111 - Aust For profit |
Category | 3111 |
UON | Y |
20183 grants / $2,235,745
Accumulation, Storage, and Release of Sperm in the Oviduct$1,612,829
Funding body: National Institutes of Health
Funding body | National Institutes of Health |
---|---|
Project Team | David Miller, Brett Nixon, Randy Prather, Nicolai Bovin |
Scheme | MRS Research Grant |
Role | Investigator |
Funding Start | 2018 |
Funding Finish | 2022 |
GNo | |
Type Of Funding | International - Competitive |
Category | 3IFA |
UON | N |
Elucidating the role of epididymosomes in the transfer of fertility-modulating proteins and regulatory classes of RNA to maturing spermatozoa$547,155
Funding body: NHMRC (National Health & Medical Research Council)
Funding body | NHMRC (National Health & Medical Research Council) |
---|---|
Project Team | Professor Brett Nixon, Associate Professor Matt Dun |
Scheme | Project Grant |
Role | Lead |
Funding Start | 2018 |
Funding Finish | 2020 |
GNo | G1700434 |
Type Of Funding | Aust Competitive - Commonwealth |
Category | 1CS |
UON | Y |
Capillary Flow Two Dimensional High Pressure Liquid Chromatography (HPLC) system$75,761
Funding body: NHMRC (National Health & Medical Research Council)
Funding body | NHMRC (National Health & Medical Research Council) |
---|---|
Project Team | Professor Brett Nixon, Associate Professor Matt Dun, Associate Professor Nikki Verrills, Professor Hubert Hondermarck, Associate Professor Mark Baker, Doctor Elizabeth Bromfield |
Scheme | Equipment Grant |
Role | Lead |
Funding Start | 2018 |
Funding Finish | 2018 |
GNo | G1800470 |
Type Of Funding | Scheme excluded from IGS |
Category | EXCL |
UON | Y |
20174 grants / $23,400
Jennie Thomas Medical Research Travel Grant$8,900
Funding body: Hunter Medical Research Institute
Funding body | Hunter Medical Research Institute |
---|---|
Project Team | Miss Jacinta Martin, Professor Brett Nixon, Laureate Professor John Aitken, Doctor Elizabeth Bromfield |
Scheme | Jennie Thomas Medical Research Travel Grant |
Role | Lead |
Funding Start | 2017 |
Funding Finish | 2017 |
GNo | G1701521 |
Type Of Funding | C3120 - Aust Philanthropy |
Category | 3120 |
UON | Y |
Jennie Thomas Travel Award$7,000
Funding body: Hunter Medical Research Institute
Funding body | Hunter Medical Research Institute |
---|---|
Project Team | Miss Bettina Mihalas, Professor Brett Nixon, Professor Eileen McLaughlin |
Scheme | Jennie Thomas Medical Research Travel Grant |
Role | Lead |
Funding Start | 2017 |
Funding Finish | 2017 |
GNo | G1701509 |
Type Of Funding | C3120 - Aust Philanthropy |
Category | 3120 |
UON | Y |
Fertilisation Induced Changes to Oocyte Protective Machinery: Investigating Chemotherapeutics $5,000
Funding body: Hunter Medical Research Institute
Funding body | Hunter Medical Research Institute |
---|---|
Project Team | Professor Brett Nixon, Laureate Professor John Aitken, Doctor Elizabeth Bromfield, Miss Jacinta Martin |
Scheme | Greaves Family Postgraduate Top Up Scholarship in Medical Research |
Role | Lead |
Funding Start | 2017 |
Funding Finish | 2017 |
GNo | G1700388 |
Type Of Funding | C3120 - Aust Philanthropy |
Category | 3120 |
UON | Y |
Effects of acrylamide on the male germline$2,500
Funding body: Hunter Medical Research Institute
Funding body | Hunter Medical Research Institute |
---|---|
Project Team | Doctor Shaun Roman, Professor Brett Nixon, Miss Caitlin Chambers |
Scheme | HMRI Student Association 'Future' Postgraduate Medical Research Scholarship |
Role | Investigator |
Funding Start | 2017 |
Funding Finish | 2017 |
GNo | G1700585 |
Type Of Funding | C3120 - Aust Philanthropy |
Category | 3120 |
UON | Y |
20164 grants / $1,123,370
The role of dynamin in spermatogenesis, sperm maturation and sperm-oocyte interactions$567,478
Funding body: NHMRC (National Health & Medical Research Council)
Funding body | NHMRC (National Health & Medical Research Council) |
---|---|
Project Team | Professor Brett Nixon, Professor Eileen McLaughlin |
Scheme | Project Grant |
Role | Lead |
Funding Start | 2016 |
Funding Finish | 2018 |
GNo | G1500178 |
Type Of Funding | Aust Competitive - Commonwealth |
Category | 1CS |
UON | Y |
Investigation of the mechanisms underpinning HSPA2 dysfunction in the spermatozoa of infertile patients$494,958
Funding body: NHMRC (National Health & Medical Research Council)
Funding body | NHMRC (National Health & Medical Research Council) |
---|---|
Project Team | Professor Brett Nixon, Laureate Professor John Aitken, Professor Eileen McLaughlin |
Scheme | Project Grant |
Role | Lead |
Funding Start | 2016 |
Funding Finish | 2018 |
GNo | G1500165 |
Type Of Funding | Aust Competitive - Commonwealth |
Category | 1CS |
UON | Y |
Beckman Coulter Optima Max-TL, Benchtop Ultra Centrifuge, TLA-110 Fixed-Angle Rotor Package, TLS-55 Swinging Bucket Rotor Package$40,934
Funding body: NHMRC (National Health & Medical Research Council)
Funding body | NHMRC (National Health & Medical Research Council) |
---|---|
Project Team | Professor Brett Nixon, Laureate Professor John Aitken, Professor Eileen McLaughlin, Associate Professor Mark Baker, Doctor Jessie Sutherland, Doctor Elizabeth Bromfield |
Scheme | Equipment Grant |
Role | Lead |
Funding Start | 2016 |
Funding Finish | 2016 |
GNo | G1601314 |
Type Of Funding | C2120 - Aust Commonwealth - Other |
Category | 2120 |
UON | Y |
Emlyn and Jennie Thomas Postgraduate Medical Research Scholarship$20,000
Funding body: Hunter Medical Research Institute
Funding body | Hunter Medical Research Institute |
---|---|
Project Team | Miss Bettina Mihalas, Professor Brett Nixon, Professor Eileen McLaughlin |
Scheme | Postgraduate Research Scholarship |
Role | Lead |
Funding Start | 2016 |
Funding Finish | 2017 |
GNo | G1601053 |
Type Of Funding | C3120 - Aust Philanthropy |
Category | 3120 |
UON | Y |
20151 grants / $417,343
Use of quinone adducted peptides in the regulation of fertility$417,343
Funding body: ARC (Australian Research Council)
Funding body | ARC (Australian Research Council) |
---|---|
Project Team | Laureate Professor John Aitken, Professor Brett Nixon |
Scheme | Discovery Projects |
Role | Investigator |
Funding Start | 2015 |
Funding Finish | 2017 |
GNo | G1400318 |
Type Of Funding | Aust Competitive - Commonwealth |
Category | 1CS |
UON | Y |
20148 grants / $1,490,388
Investigation of novel mechanisms for the regulation of sperm-oocyte interactions$901,056
Funding body: ARC (Australian Research Council)
Funding body | ARC (Australian Research Council) |
---|---|
Project Team | Professor Brett Nixon |
Scheme | Future Fellowships |
Role | Lead |
Funding Start | 2014 |
Funding Finish | 2018 |
GNo | G1301259 |
Type Of Funding | Aust Competitive - Commonwealth |
Category | 1CS |
UON | Y |
miRNA regulation of sperm maturation $396,157
Funding body: NHMRC (National Health & Medical Research Council)
Funding body | NHMRC (National Health & Medical Research Council) |
---|---|
Project Team | Professor Brett Nixon, Professor Eileen McLaughlin, Doctor Janet Bristow |
Scheme | Project Grant |
Role | Lead |
Funding Start | 2014 |
Funding Finish | 2016 |
GNo | G1300125 |
Type Of Funding | Aust Competitive - Commonwealth |
Category | 1CS |
UON | Y |
JuLI Stage $71,674
Funding body: NHMRC (National Health & Medical Research Council)
Funding body | NHMRC (National Health & Medical Research Council) |
---|---|
Project Team | Professor Pradeep Tanwar, Professor Eileen McLaughlin, Conjoint Professor Robin Callister, Professor Xu Dong Zhang, Professor Murray Cairns, Professor Brett Nixon, Professor Hubert Hondermarck, Associate Professor Phillip Dickson, Associate Professor Nikki Verrills, Associate Professor Matt Dun, Doctor Jessie Sutherland, Doctor Janani Kumar, Associate Professor Jay Horvat, Associate Professor Susan Hua, Professor Liz Milward, Associate Professor Estelle Sontag, Professor Dirk Van Helden, Doctor Janet Bristow, Doctor Jean-Marie Sontag |
Scheme | Equipment Grant |
Role | Investigator |
Funding Start | 2014 |
Funding Finish | 2014 |
GNo | G1500860 |
Type Of Funding | Other Public Sector - Commonwealth |
Category | 2OPC |
UON | Y |
DVC(R) Research Support for Future Fellow (FT14)$59,501
Funding body: University of Newcastle
Funding body | University of Newcastle |
---|---|
Project Team | Professor Brett Nixon |
Scheme | Future Fellowship Support |
Role | Lead |
Funding Start | 2014 |
Funding Finish | 2018 |
GNo | G1400948 |
Type Of Funding | Internal |
Category | INTE |
UON | Y |
The Role of the GTPase Dynamin in the Female Germline$30,000
Funding body: Hunter Medical Research Institute
Funding body | Hunter Medical Research Institute |
---|---|
Project Team | Doctor Kate Redgrove, Doctor Janet Bristow, Professor Eileen McLaughlin, Professor Brett Nixon |
Scheme | Bridging Grant |
Role | Investigator |
Funding Start | 2014 |
Funding Finish | 2014 |
GNo | G1301333 |
Type Of Funding | Grant - Aust Non Government |
Category | 3AFG |
UON | Y |
The role of dynamin is spermatogenesis and sperm-oocyte interactions$20,000
Funding body: University of Newcastle
Funding body | University of Newcastle |
---|---|
Project Team | Professor Brett Nixon, Professor Eileen McLaughlin, Professor Adam McCluskey |
Scheme | Near Miss Grant |
Role | Lead |
Funding Start | 2014 |
Funding Finish | 2014 |
GNo | G1301400 |
Type Of Funding | Internal |
Category | INTE |
UON | Y |
Development of contraception on demand $10,000
Funding body: University of Newcastle
Funding body | University of Newcastle |
---|---|
Project Team | Professor Brett Nixon, Laureate Professor John Aitken |
Scheme | Near Miss Grant |
Role | Lead |
Funding Start | 2014 |
Funding Finish | 2014 |
GNo | G1301399 |
Type Of Funding | Internal |
Category | INTE |
UON | Y |
Faculty PVC Conference Assistance Grant 2014$2,000
Funding body: University of Newcastle - Faculty of Science & IT
Funding body | University of Newcastle - Faculty of Science & IT |
---|---|
Project Team | Professor Brett Nixon |
Scheme | PVC Conference Assistance Grant |
Role | Lead |
Funding Start | 2014 |
Funding Finish | 2014 |
GNo | G1401230 |
Type Of Funding | Internal |
Category | INTE |
UON | Y |
20136 grants / $381,182
Novel function of heat shock protein 2A in the regulation of human sperm-egg interactions$292,182
Funding body: NHMRC (National Health & Medical Research Council)
Funding body | NHMRC (National Health & Medical Research Council) |
---|---|
Project Team | Professor Brett Nixon, Laureate Professor John Aitken, Professor Eileen McLaughlin |
Scheme | Project Grant |
Role | Lead |
Funding Start | 2013 |
Funding Finish | 2015 |
GNo | G1200193 |
Type Of Funding | Aust Competitive - Commonwealth |
Category | 1CS |
UON | Y |
Leica TP 1020 Automatic Tissue Processor for histology applications$35,000
Funding body: NHMRC (National Health & Medical Research Council)
Funding body | NHMRC (National Health & Medical Research Council) |
---|---|
Project Team | Professor Pradeep Tanwar, Professor Eileen McLaughlin, Professor Xu Dong Zhang, Conjoint Professor Robert Callister, Associate Professor Phillip Dickson, Professor Hubert Hondermarck, Doctor Jean-Marie Sontag, Professor Dirk Van Helden, Associate Professor Doug Smith, Associate Professor Phil Jobling, Associate Professor Estelle Sontag, Associate Professor Paul Tooney, Associate Professor Susan Hua, Doctor Janet Bristow, Associate Professor Jay Horvat, Professor Liz Milward, Professor Adam McCluskey, Professor Brett Nixon, Associate Professor Rebecca Lim, Professor Alan Brichta |
Scheme | Equipment Grant |
Role | Investigator |
Funding Start | 2013 |
Funding Finish | 2013 |
GNo | G1201185 |
Type Of Funding | Other Public Sector - Commonwealth |
Category | 2OPC |
UON | Y |
Novel mechanisms for the regulation of sperm-oocyte interactions$20,000
Funding body: University of Newcastle
Funding body | University of Newcastle |
---|---|
Project Team | Professor Brett Nixon |
Scheme | Near Miss Grant |
Role | Lead |
Funding Start | 2013 |
Funding Finish | 2013 |
GNo | G1300458 |
Type Of Funding | Internal |
Category | INTE |
UON | Y |
NucleoCounter SP100$14,000
Funding body: NHMRC (National Health & Medical Research Council)
Funding body | NHMRC (National Health & Medical Research Council) |
---|---|
Project Team | Laureate Professor John Aitken, Professor Brett Nixon, Doctor Shaun Roman, Associate Professor Mark Baker, Doctor Geoffry De Iuliis, Doctor Lisa Mitchell, Doctor Matthew Jobling, Doctor Zamira Gibb, Mr Nenad Naumovski |
Scheme | Equipment Grant |
Role | Investigator |
Funding Start | 2013 |
Funding Finish | 2013 |
GNo | G1201175 |
Type Of Funding | Other Public Sector - Commonwealth |
Category | 2OPC |
UON | Y |
Dynamin - role in sperm oocyte interaction$10,000
Funding body: University of Newcastle
Funding body | University of Newcastle |
---|---|
Project Team | Professor Eileen McLaughlin, Professor Brett Nixon, Professor Adam McCluskey |
Scheme | Near Miss Grant |
Role | Investigator |
Funding Start | 2013 |
Funding Finish | 2013 |
GNo | G1300483 |
Type Of Funding | Internal |
Category | INTE |
UON | Y |
Dynamin - role in sperm oocyte interaction$10,000
Funding body: Hunter Medical Research Institute
Funding body | Hunter Medical Research Institute |
---|---|
Project Team | Professor Eileen McLaughlin, Professor Brett Nixon, Professor Adam McCluskey |
Scheme | Near Miss |
Role | Investigator |
Funding Start | 2013 |
Funding Finish | 2013 |
GNo | G1300700 |
Type Of Funding | C3112 - Aust Not for profit |
Category | 3112 |
UON | Y |
20122 grants / $17,447
The role of miRNAs in the regulation of sperm maturation$10,000
Funding body: University of Newcastle - Faculty of Science & IT
Funding body | University of Newcastle - Faculty of Science & IT |
---|---|
Project Team | Professor Brett Nixon, Professor Eileen McLaughlin, Doctor Natalie Beveridge |
Scheme | Strategic Initiative Research Fund (SIRF) |
Role | Lead |
Funding Start | 2012 |
Funding Finish | 2012 |
GNo | G1401028 |
Type Of Funding | Internal |
Category | INTE |
UON | Y |
Faculty Visiting Fellowship 2012$7,447
Funding body: University of Newcastle - Faculty of Science & IT
Funding body | University of Newcastle - Faculty of Science & IT |
---|---|
Project Team | Professor Brett Nixon |
Scheme | Visiting Fellowship |
Role | Lead |
Funding Start | 2012 |
Funding Finish | 2012 |
GNo | G1401130 |
Type Of Funding | Internal |
Category | INTE |
UON | Y |
20112 grants / $40,000
SLX4 - a key regulator of male germ cell development and DNA repair$25,000
Funding body: Hunter Medical Research Institute
Funding body | Hunter Medical Research Institute |
---|---|
Project Team | Professor Eileen McLaughlin, Professor Brett Nixon |
Scheme | Project Grant |
Role | Investigator |
Funding Start | 2011 |
Funding Finish | 2011 |
GNo | G1001000 |
Type Of Funding | Contract - Aust Non Government |
Category | 3AFC |
UON | Y |
Eppendorf mastercycler pro with thermomixer comfort and 5430R centrifuge$15,000
Funding body: NHMRC (National Health & Medical Research Council)
Funding body | NHMRC (National Health & Medical Research Council) |
---|---|
Project Team | Professor Eileen McLaughlin, Professor Peter Lewis, Professor Adam McCluskey, Conjoint Professor Keith Jones, Professor Brett Nixon, Doctor Shaun Roman, Doctor Jennette Sakoff, Associate Professor Ian Grainge, Doctor Janet Bristow, Doctor Xiao Yang |
Scheme | Equipment Grant |
Role | Investigator |
Funding Start | 2011 |
Funding Finish | 2011 |
GNo | G1100028 |
Type Of Funding | Other Public Sector - Commonwealth |
Category | 2OPC |
UON | Y |
20107 grants / $1,695,000
An Integrated LC-MS-NMR facility for Applications in Proteomics and Organic Chemistry$500,000
Funding body: ARC (Australian Research Council)
Funding body | ARC (Australian Research Council) |
---|---|
Project Team | Professor Adam McCluskey, Laureate Professor John Aitken, Professor Paul Dastoor, Professor Phillip Robinson, Professor Eileen McLaughlin, Emeritus Professor Geoffrey Lawrance, Emeritus Professor Marcel Maeder, Professor Hugh Dunstan, Doctor Shaun Roman, Conjoint Professor Rob Atkin, Associate Professor Clovia Holdsworth, Associate Professor Mark Baker, Associate Professor Nikki Verrills, Professor Gottfried Otting, Professor Brett Nixon, Doctor Xiaojing Zhou, Ms Megan Chircop, Doctor Warwick Belcher |
Scheme | Linkage Infrastructure Equipment & Facilities (LIEF) |
Role | Investigator |
Funding Start | 2010 |
Funding Finish | 2010 |
GNo | G0190402 |
Type Of Funding | Scheme excluded from IGS |
Category | EXCL |
UON | Y |
Laser microdissection microscopy system for cell and development biology$350,000
Funding body: ARC (Australian Research Council)
Funding body | ARC (Australian Research Council) |
---|---|
Project Team | Professor Eileen McLaughlin, Conjoint Professor Keith Jones, Laureate Professor John Aitken, Professor Brett Nixon, Doctor Shaun Roman, Professor Alan Brichta, Doctor Rick Thorne, Associate Professor Doug Smith, Associate Professor David McCurdy, Emeritus Professor Ray Rose, Professor Christopher Grof, Emeritus Professor Leonie Ashman, Professor Gordon Burns, Associate Professor Brett Graham, Associate Professor Paul Tooney, Laureate Professor Roger Smith, Laureate Professor Paul Foster, Professor Trevor Day, Conjoint Professor Robert Callister |
Scheme | Linkage Infrastructure Equipment & Facilities (LIEF) |
Role | Investigator |
Funding Start | 2010 |
Funding Finish | 2010 |
GNo | G0190369 |
Type Of Funding | Scheme excluded from IGS |
Category | EXCL |
UON | Y |
An Integrated LC-MS-NMR facility for Applications in Proteomics and Organic Chemistry$280,000
Funding body: University of Newcastle
Funding body | University of Newcastle |
---|---|
Project Team | Professor Adam McCluskey, Laureate Professor John Aitken, Professor Paul Dastoor, Professor Phillip Robinson, Professor Eileen McLaughlin, Emeritus Professor Geoffrey Lawrance, Emeritus Professor Marcel Maeder, Professor Hugh Dunstan, Doctor Shaun Roman, Conjoint Professor Rob Atkin, Associate Professor Clovia Holdsworth, Associate Professor Mark Baker, Associate Professor Nikki Verrills, Professor Gottfried Otting, Professor Brett Nixon, Doctor Xiaojing Zhou, Ms Megan Chircop, Doctor Warwick Belcher |
Scheme | Equipment Grant |
Role | Investigator |
Funding Start | 2010 |
Funding Finish | 2010 |
GNo | G1000873 |
Type Of Funding | Internal |
Category | INTE |
UON | Y |
Monotremes provide a key to understanding the molecular biology of mammalian gamete evolution$270,000
Funding body: ARC (Australian Research Council)
Funding body | ARC (Australian Research Council) |
---|---|
Project Team | Professor Brett Nixon, Dr Heath Ecroyd, Conjoint Associate Professor Russell Jones |
Scheme | Discovery Projects |
Role | Lead |
Funding Start | 2010 |
Funding Finish | 2012 |
GNo | G0190056 |
Type Of Funding | Aust Competitive - Commonwealth |
Category | 1CS |
UON | Y |
Laser microdissection microscopy system for cell and development biology$215,000
Funding body: University of Newcastle
Funding body | University of Newcastle |
---|---|
Project Team | Professor Eileen McLaughlin, Conjoint Professor Keith Jones, Laureate Professor John Aitken, Professor Brett Nixon, Doctor Shaun Roman, Professor Alan Brichta, Doctor Rick Thorne, Associate Professor Doug Smith, Associate Professor David McCurdy, Emeritus Professor Ray Rose, Professor Christopher Grof, Emeritus Professor Leonie Ashman, Professor Gordon Burns, Associate Professor Brett Graham, Associate Professor Paul Tooney, Laureate Professor Roger Smith, Laureate Professor Paul Foster, Professor Trevor Day, Conjoint Professor Robert Callister |
Scheme | Equipment Grant |
Role | Investigator |
Funding Start | 2010 |
Funding Finish | 2010 |
GNo | G1000874 |
Type Of Funding | Internal |
Category | INTE |
UON | Y |
Laser microdissection microscopy system for cell and development biology (HMRI contribution towards 2010 ARC LIEF grant)$50,000
Funding body: Hunter Medical Research Institute
Funding body | Hunter Medical Research Institute |
---|---|
Project Team | Professor Eileen McLaughlin, Conjoint Professor Keith Jones, Laureate Professor John Aitken, Professor Brett Nixon, Doctor Shaun Roman, Professor Alan Brichta, Doctor Rick Thorne, Associate Professor Doug Smith, Associate Professor David McCurdy, Emeritus Professor Ray Rose, Professor Christopher Grof, Emeritus Professor Leonie Ashman, Professor Gordon Burns, Associate Professor Brett Graham, Associate Professor Paul Tooney, Laureate Professor Roger Smith, Laureate Professor Paul Foster, Professor Trevor Day, Conjoint Professor Robert Callister |
Scheme | Equipment Grant |
Role | Investigator |
Funding Start | 2010 |
Funding Finish | 2010 |
GNo | G1000144 |
Type Of Funding | Other Public Sector - State |
Category | 2OPS |
UON | Y |
Waters, Ultra high performance liquid chromatography system (nano-Aquity) $30,000
Funding body: NHMRC (National Health & Medical Research Council)
Funding body | NHMRC (National Health & Medical Research Council) |
---|---|
Project Team | Associate Professor Mark Baker, Laureate Professor John Aitken, Professor Adam McCluskey, Professor Brett Nixon, Emeritus Professor John Rostas, Professor Eileen McLaughlin |
Scheme | Equipment Grant |
Role | Investigator |
Funding Start | 2010 |
Funding Finish | 2010 |
GNo | G1000051 |
Type Of Funding | Other Public Sector - Commonwealth |
Category | 2OPC |
UON | Y |
20095 grants / $1,319,000
An Advanced Mass Spectrometry Facility for Applications in Proteomics and Organic Chemistry$495,000
Funding body: ARC (Australian Research Council)
Characterization of the molecular basis of human sperm-oocyte interaction$474,000
Funding body: NHMRC (National Health & Medical Research Council)
Funding body | NHMRC (National Health & Medical Research Council) |
---|---|
Project Team | Professor Brett Nixon, Professor Eileen McLaughlin, Dr M O'Bryan |
Scheme | Project Grant |
Role | Lead |
Funding Start | 2009 |
Funding Finish | 2011 |
GNo | G0188867 |
Type Of Funding | Aust Competitive - Commonwealth |
Category | 1CS |
UON | Y |
Confocal Laser Scanning Microscopy for Live Cell Imaging$275,000
Funding body: ARC (Australian Research Council)
Funding body | ARC (Australian Research Council) |
---|---|
Project Team | Conjoint Professor Keith Jones, Professor Eileen McLaughlin, Laureate Professor John Aitken, Emeritus Professor Ray Rose, Emeritus Professor John Patrick, Conjoint Professor Christina Offler, Associate Professor David McCurdy, Emeritus Professor Leonie Ashman, Professor Gordon Burns, Professor Dirk Van Helden, Associate Professor Nikki Verrills, Professor Brett Nixon, Doctor Shaun Roman, Professor Yong-Ling Ruan, Doctor Rick Thorne, Prof MIKE Calford |
Scheme | Linkage Infrastructure Equipment & Facilities (LIEF) |
Role | Investigator |
Funding Start | 2009 |
Funding Finish | 2009 |
GNo | G0189038 |
Type Of Funding | Scheme excluded from IGS |
Category | EXCL |
UON | Y |
An Advanced Mass Spectrometry Facility for Applications in Proteomics and Organic Chemistry$50,000
Funding body: ARC (Australian Research Council)
LAS-4000 Chemiluminescence imaging system$25,000
Funding body: NHMRC (National Health & Medical Research Council)
Funding body | NHMRC (National Health & Medical Research Council) |
---|---|
Project Team | Professor Eileen McLaughlin, Laureate Professor John Aitken, Professor Peter Lewis, Professor Brett Nixon, Doctor Shaun Roman |
Scheme | Equipment Grant |
Role | Investigator |
Funding Start | 2009 |
Funding Finish | 2009 |
GNo | G0189852 |
Type Of Funding | Other Public Sector - Commonwealth |
Category | 2OPC |
UON | Y |
20084 grants / $405,060
Xenobiotics - ocidative stress in the mammalian ovary$362,625
Funding body: NHMRC (National Health & Medical Research Council)
Funding body | NHMRC (National Health & Medical Research Council) |
---|---|
Project Team | Professor Eileen McLaughlin, Professor Brett Nixon, Doctor Shaun Roman |
Scheme | Project Grant |
Role | Investigator |
Funding Start | 2008 |
Funding Finish | 2010 |
GNo | G0187620 |
Type Of Funding | Aust Competitive - Commonwealth |
Category | 1CS |
UON | Y |
Understanding the aetiology of human male infertility: Elucidation of the mechanisms underpinning sperm-egg interaction$21,650
Funding body: Hunter Medical Research Institute
Funding body | Hunter Medical Research Institute |
---|---|
Project Team | Professor Brett Nixon, Professor Eileen McLaughlin |
Scheme | Project Grant |
Role | Lead |
Funding Start | 2008 |
Funding Finish | 2008 |
GNo | G0188465 |
Type Of Funding | Contract - Aust Non Government |
Category | 3AFC |
UON | Y |
Characterization of the molecular basis of mouse sperm-oocyte interaction$18,285
Funding body: University of Newcastle
Funding body | University of Newcastle |
---|---|
Project Team | Professor Brett Nixon, Professor Eileen McLaughlin |
Scheme | Near Miss Grant |
Role | Lead |
Funding Start | 2008 |
Funding Finish | 2008 |
GNo | G0188405 |
Type Of Funding | Internal |
Category | INTE |
UON | Y |
41st Annual Meeting of the Society for the Study of Reproduction $2,500
Funding body: University of Newcastle
Funding body | University of Newcastle |
---|---|
Project Team | Professor Brett Nixon |
Scheme | Travel Grant |
Role | Lead |
Funding Start | 2008 |
Funding Finish | 2008 |
GNo | G0188643 |
Type Of Funding | Internal |
Category | INTE |
UON | Y |
20074 grants / $51,471
Chemokines and spermatogenesis: roles of SDF-1 and CXCR-4 in the establishment of spermatogenesis$20,000
Funding body: University of Newcastle
Funding body | University of Newcastle |
---|---|
Project Team | Professor Eileen McLaughlin, Doctor Shaun Roman, Professor Brett Nixon |
Scheme | Near Miss Grant |
Role | Investigator |
Funding Start | 2007 |
Funding Finish | 2007 |
GNo | G0187197 |
Type Of Funding | Internal |
Category | INTE |
UON | Y |
Determination of the molecular processes involved in post-testicular development of sperm in the platypus and their relationship with sperm competition$16,471
Funding body: University of Newcastle
Funding body | University of Newcastle |
---|---|
Project Team | Professor Brett Nixon, Conjoint Associate Professor Russell Jones |
Scheme | Near Miss Grant |
Role | Lead |
Funding Start | 2007 |
Funding Finish | 2007 |
GNo | G0187188 |
Type Of Funding | Internal |
Category | INTE |
UON | Y |
Validation of novel model to explain the functional activation of mouse spermatozoa$7,998
Funding body: University of Newcastle
Funding body | University of Newcastle |
---|---|
Project Team | Professor Brett Nixon |
Scheme | Pilot Grant |
Role | Lead |
Funding Start | 2007 |
Funding Finish | 2007 |
GNo | G0187878 |
Type Of Funding | Internal |
Category | INTE |
UON | Y |
Validation of novel model to explain the functional activation of mouse spermatozoa$7,002
Funding body: University of Newcastle - Faculty of Science & IT
Funding body | University of Newcastle - Faculty of Science & IT |
---|---|
Project Team | Professor Brett Nixon |
Scheme | Pilot Grant |
Role | Lead |
Funding Start | 2007 |
Funding Finish | 2007 |
GNo | G0188360 |
Type Of Funding | Internal |
Category | INTE |
UON | Y |
20061 grants / $544,282
PRC - Priority Research Centre for Reproductive Science$544,282
Funding body: University of Newcastle
Funding body | University of Newcastle |
---|---|
Project Team | Laureate Professor John Aitken, Laureate Professor Roger Smith, Professor Eileen McLaughlin, Professor Brett Nixon, Doctor Shaun Roman, Conjoint Associate Professor Vicki Clifton, Conjoint Professor Warwick Giles, Professor Jon Hirst, Conjoint Associate Professor Rick Nicholson, Professor Ian Symonds |
Scheme | Priority Research Centre |
Role | Investigator |
Funding Start | 2006 |
Funding Finish | 2013 |
GNo | G0186945 |
Type Of Funding | Internal |
Category | INTE |
UON | Y |
20051 grants / $9,000
The initiation of fertilisation: Characterisation of the sperm receptor complex for the oocyte$9,000
Funding body: University of Newcastle
Funding body | University of Newcastle |
---|---|
Project Team | Professor Brett Nixon, Laureate Professor John Aitken |
Scheme | Project Grant |
Role | Lead |
Funding Start | 2005 |
Funding Finish | 2005 |
GNo | G0184742 |
Type Of Funding | Internal |
Category | INTE |
UON | Y |
20043 grants / $29,000
Characterisation of a novel recepto-mediated signal transduction pathway in human spermatozoa.$14,000
Funding body: University of Newcastle
Funding body | University of Newcastle |
---|---|
Project Team | Professor Brett Nixon, Emeritus Professor Leonie Ashman |
Scheme | Project Grant |
Role | Lead |
Funding Start | 2004 |
Funding Finish | 2004 |
GNo | G0183444 |
Type Of Funding | Internal |
Category | INTE |
UON | Y |
Molecular basis of sperm-egg interaction: Identification of the receptor complex for ZP3$8,000
Funding body: University of Newcastle
Funding body | University of Newcastle |
---|---|
Project Team | Professor Brett Nixon, Doctor Benjamin Curry |
Scheme | Project Grant |
Role | Lead |
Funding Start | 2004 |
Funding Finish | 2004 |
GNo | G0183415 |
Type Of Funding | Internal |
Category | INTE |
UON | Y |
The role of the father in recurrent early pregnancy loss$7,000
Funding body: Hunter Medical Research Institute
Funding body | Hunter Medical Research Institute |
---|---|
Project Team | Professor Brett Nixon |
Scheme | Adamstown Lions Club |
Role | Lead |
Funding Start | 2004 |
Funding Finish | 2004 |
GNo | G0183756 |
Type Of Funding | Contract - Aust Non Government |
Category | 3AFC |
UON | Y |
20021 grants / $12,000
The role of protein tyrosine phosphorylation in mouse sperm-oocyte interaction$12,000
Funding body: University of Newcastle
Funding body | University of Newcastle |
---|---|
Project Team | Professor Brett Nixon |
Scheme | Early Career Researcher Grant |
Role | Lead |
Funding Start | 2002 |
Funding Finish | 2002 |
GNo | G0182255 |
Type Of Funding | Internal |
Category | INTE |
UON | Y |
20011 grants / $15,000
The role of protein tyrosine phosphorylation in mouse sperm-oocyte interaction$15,000
Funding body: University of Newcastle
Funding body | University of Newcastle |
---|---|
Project Team | Professor Brett Nixon |
Scheme | Early Career Researcher Grant |
Role | Lead |
Funding Start | 2001 |
Funding Finish | 2001 |
GNo | G0180831 |
Type Of Funding | Internal |
Category | INTE |
UON | Y |
Research Supervision
Number of supervisions
Current Supervision
Commenced | Level of Study | Research Title | Program | Supervisor Type |
---|---|---|---|---|
2021 | Masters | Neoantigen Immunopeptidomics for the Development of Immunotherapies for the Treatment of Diffuse Intrinsic Pontine Glioma | M Philosophy (Medical Biochem), College of Health, Medicine and Wellbeing, The University of Newcastle | Co-Supervisor |
2020 | PhD | Exploring ALOX15 as a Molecular Conduit between Infertility and Systemic Ill Health in Men | PhD (Biological Sciences), College of Engineering, Science and Environment, The University of Newcastle | Co-Supervisor |
2020 | PhD | Improving Development of Cultivated Spermatogonial Stem Cells Using Novel Growth Factor Receptors | PhD (Biological Sciences), College of Engineering, Science and Environment, The University of Newcastle | Co-Supervisor |
2020 | PhD | The Diagnosis and Resolution of Oxidative Stress in the Male Germ Line | PhD (Biological Sciences), College of Engineering, Science and Environment, The University of Newcastle | Co-Supervisor |
2020 | PhD | Novel Roles for Seminal Fluid Extracellular Vesicles | PhD (Biological Sciences), College of Engineering, Science and Environment, The University of Newcastle | Co-Supervisor |
2019 | PhD | Exploring Protein Aggregation in the Male Germ Line in Response to Oxidative Stress | PhD (Biological Sciences), College of Engineering, Science and Environment, The University of Newcastle | Co-Supervisor |
2019 | PhD | The Development of Novel Materials for the Selection of High Quality Spermatozoa and the Assessment of Cellular Oxidative Status Through the Analysis of Lipid Peroxide Metabolites | PhD (Biological Sciences), College of Engineering, Science and Environment, The University of Newcastle | Co-Supervisor |
2019 | PhD | The Role of Autophagy in Oocyte Development and Ageing | PhD (Medical Biochemistry), College of Health, Medicine and Wellbeing, The University of Newcastle | Co-Supervisor |
2019 | Masters | Developing a Novel Diagnostic Test for Early Pregnancy of the Mare and Find out the Embryo Endometrial Interaction | M Philosophy (Biological Sc), College of Engineering, Science and Environment, The University of Newcastle | Principal Supervisor |
2019 | Masters | The Application of Chemical Cross-linking Mass Spectrometry towards Understanding the Protein Interactome in Human Spermatozoa | M Philosophy (Biological Sc), College of Engineering, Science and Environment, The University of Newcastle | Co-Supervisor |
2017 | PhD | Development of a Novel Injectable Chemical Agent for the Sterilisation of Cats and Dogs | PhD (Biological Sciences), College of Engineering, Science and Environment, The University of Newcastle | Co-Supervisor |
2017 | PhD | The Impact of Environmental Insult on Mouse Epididymal Spermatozoa | PhD (Biological Sciences), College of Engineering, Science and Environment, The University of Newcastle | Principal Supervisor |
2017 | PhD | Regulation of Sperm Function and Oxidative Stress by Lipoxygenase Enzymes | PhD (Biological Sciences), College of Engineering, Science and Environment, The University of Newcastle | Co-Supervisor |
Past Supervision
Year | Level of Study | Research Title | Program | Supervisor Type |
---|---|---|---|---|
2020 | PhD | The Impact of the Environmental Hazard, Acrylamide, on the Male Reproductive Tract and Transgenerational Phenotype | PhD (Biological Sciences), College of Engineering, Science and Environment, The University of Newcastle | Co-Supervisor |
2019 | PhD | Molecular Mechanisms Contributing to the Age-Related Decline in Oocyte Quality | PhD (Biological Sciences), College of Engineering, Science and Environment, The University of Newcastle | Principal Supervisor |
2019 | PhD | Quality Control Mechanisms Responsible for the Maintenance of Genomic Integrity in the Female Germline | PhD (Biological Sciences), College of Engineering, Science and Environment, The University of Newcastle | Principal Supervisor |
2019 | PhD | Functional Characterisation of Dynamin in Spermatozoa Epididymal Maturation and Acrosomal Exocytosis | PhD (Biological Sciences), College of Engineering, Science and Environment, The University of Newcastle | Principal Supervisor |
2018 | PhD | Investigating the Effects of Mobile Phone Radiation and Heating on Spermatogenesis and Sperm Function | PhD (Biological Sciences), College of Engineering, Science and Environment, The University of Newcastle | Co-Supervisor |
2018 | PhD | Post-Meiotic DNA Damage in Spermatozoa and Consequences to Offspring | PhD (Biological Sciences), College of Engineering, Science and Environment, The University of Newcastle | Co-Supervisor |
2018 | Masters | Investigating the Profile of miRNAs in the Mammalian Male Reproductive Tract | M Philosophy (Biological Sc), College of Engineering, Science and Environment, The University of Newcastle | Principal Supervisor |
2017 | PhD | The Consequences of Paternal Acrylamide Exposure and Potential for Amelioration | PhD (Biological Sciences), College of Engineering, Science and Environment, The University of Newcastle | Co-Supervisor |
2017 | PhD | Investigation of Equine Fertility Mechanisms through Proteomic Analysis of Stallion Spermatozoa, Early Equine Embryos and Pregnant Mare Uterine Fluid | PhD (Biological Sciences), College of Engineering, Science and Environment, The University of Newcastle | Co-Supervisor |
2017 | PhD | Towards Non-Surgical Sterilisation of Feral Horses: Biomarkers of Folliculogenesis and Covalent Modification of Proteins Using Electrophilic Aldehydes | PhD (Biological Sciences), College of Engineering, Science and Environment, The University of Newcastle | Co-Supervisor |
2016 | Masters | Insulin Production in the Mouse Uterus | M Philosophy (Biological Sc), College of Engineering, Science and Environment, The University of Newcastle | Co-Supervisor |
2015 | PhD | Functional Characterisation of Dynamin in Mouse Spermatozoa | PhD (Biological Sciences), College of Engineering, Science and Environment, The University of Newcastle | Principal Supervisor |
2015 | PhD | The Function of Molecular Chaperones in Human Sperm-Egg Recognition | PhD (Biological Sciences), College of Engineering, Science and Environment, The University of Newcastle | Principal Supervisor |
2015 | PhD | The Role of Reactive Oxygen Species and Oxidative Stress in Post-Ovulatory Ageing and Apoptosis of the Mammalian Oocyte | PhD (Biological Sciences), College of Engineering, Science and Environment, The University of Newcastle | Co-Supervisor |
2014 | PhD | The Consequences of Acrylamide Exposure of the Male Germ Line | PhD (Biological Sciences), College of Engineering, Science and Environment, The University of Newcastle | Co-Supervisor |
2013 | PhD | Regulation of the Transition from Gonocytes to Spermatogonia | PhD (Biological Sciences), College of Engineering, Science and Environment, The University of Newcastle | Co-Supervisor |
2013 | PhD | New Insights into the Molecular Basis of Human Sperm-Egg Interaction | PhD (Biological Sciences), College of Engineering, Science and Environment, The University of Newcastle | Principal Supervisor |
2012 | PhD | The Molecular Basis of Sperm - Oocyte Interactions | PhD (Biological Sciences), College of Engineering, Science and Environment, The University of Newcastle | Principal Supervisor |
2012 | PhD | Xenobiotics; Effects on Female Fertility | PhD (Biological Sciences), College of Engineering, Science and Environment, The University of Newcastle | Co-Supervisor |
2010 | PhD | Significance of Mitochondrial Reactive Oxygen Species Production in Male Infertility | PhD (Biological Sciences), College of Engineering, Science and Environment, The University of Newcastle | Co-Supervisor |
2009 | PhD | The Molecular Basis of Human Sperm-Zona Pellucida Interaction | PhD (Biological Sciences), College of Engineering, Science and Environment, The University of Newcastle | Co-Supervisor |
2007 | Masters | Identification of NADPH Oxidase in Mouse Spermatozoa | M Philosophy (Biological Sc), College of Engineering, Science and Environment, The University of Newcastle | Co-Supervisor |
2007 | PhD | The Development of a Novel Electrophoretic System for the Isolation of Human Spermatozoa | PhD (Biological Sciences), College of Engineering, Science and Environment, The University of Newcastle | Co-Supervisor |
2005 | PhD | The Molecular Basis of Sperm-Oocyte Interaction in the Mouse | PhD (Biological Sciences), College of Engineering, Science and Environment, The University of Newcastle | Co-Supervisor |
Research Collaborations
The map is a representation of a researchers co-authorship with collaborators across the globe. The map displays the number of publications against a country, where there is at least one co-author based in that country. Data is sourced from the University of Newcastle research publication management system (NURO) and may not fully represent the authors complete body of work.
Country | Count of Publications | |
---|---|---|
Australia | 149 | |
New Zealand | 17 | |
United States | 14 | |
France | 11 | |
Netherlands | 10 | |
More... |
News
NHMRC awards $9.3 million to 13 University of Newcastle projects
December 18, 2019
NHMRC awards $12.4 million to 14 Newcastle projects
December 12, 2018
Funding to develop targeted health solutions
August 13, 2018
New hope to stop the female biological clock
July 25, 2017
The male reproductive mechanism causing genetic change
August 31, 2016
Saltwater crocodile sperm may hold secret to male fertility
May 4, 2016
ARC Discovery Project funding success
November 19, 2014
Professor Brett Nixon
Position
NHMRC Research Fellow
Reproductive Science Group, Priority Research Centre for Reproductive Science
School of Environmental and Life Sciences
College of Engineering, Science and Environment
Focus area
Biological Sciences
Contact Details
brett.nixon@newcastle.edu.au | |
Phone | (02) 4921 6977 |
Fax | (02) 4921 6923 |
Office
Room | LS4.40 |
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Building | Life Sciences. |
Location | Callaghan University Drive Callaghan, NSW 2308 Australia |