Professor Brett Nixon

Professor Brett Nixon

NHMRC Reseach Fellow

School of Environmental and Life Sciences (Biological Sciences)

Spot the difference

An investigator of indomitable spirit, Professor Brett Nixon is pursuing the mysteries of sperm dysfunction from multiple, linked perspectives.

Brett Nixon highlight 

What do adult humans, wild rabbits, platypus and Australian saltwater crocodiles have in common? Plenty, according to Professor Brett Nixon. The esteemed educator and innovator studies all four seemingly disparate populations in his research, shedding light on the surprising connections between male factor infertility, contraception and the conservation of endangered animals.

"It's almost paradoxical," he remarks.

"If we can understand the cellular defects present in the sperm of infertile males, then we may be able to develop methods to replicate them in pest animal species to create a new form of contraceptive."

"Physiological insights provided by studying these cells can help us augment the success of captive breeding too."

Though there are "countless things" that can go wrong during the process of fertilisation, Brett is opting to focus mainly on its beginning. Comparing and contrasting samples of sperm that are able to bind to eggs with samples of sperm that can't, the proteomics expert is working to get a grasp on the structure, function and interactions of proteins that differ between the two.

"I specifically look for the proteins that are missing or appear to be behaving abnormally," he explains.

"They may hold a key role in helping to define the underlying cause of male infertility." 

Spanning the life sciences and medicine fields, Brett's efforts are also concentrated on improving artificial reproductive technologies (ARTs).

"This long term interest stems from early childhood experiences in which my family were using ARTs to improve the quality of our herd of beef and dairy cattle," the Gloucester native recalls.

"The basic idea was that we would collect eggs from the good mothers and put them together with the semen from the best bulls from around the world."

"The embryos generated were subsequently transplanted into surrogates."

"I still remember looking down a microscope for the first time and witnessing sperm binding to eggs and kicking off the fertilisation process – it was the most amazing sight."

The ties that bind

Brett's career began in 1995, when he undertook a PhD at the University of Newcastle. Completed in Canberra at the Invasive Animal CRC, the ambitious four-year probe centred on figuring out ways to sterilise foxes, rabbits and mice.

"I identified a handful of core proteins involved in a sperm's recognition of an egg and tried to raise an immune response against them to prevent fertilisation from happening," he elaborates.

"This approach was essentially trying to recapitulate a phenomenon seen in a subset of male infertility patients whereby their immune system attacks their sperm and prevents them from binding to eggs."

Seeking to continue his research on male factor infertility, Brett spent the next two years at Emory University in the United States.

"By the time I arrived, scholars within its Department of Cell Biology had developed a mouse model in which they had knocked out one of the key proteins believed to be involved in sperm-egg recognition," he states.

"So in theory, as the mouse can no longer produce that protein, its sperm shouldn't be able to recognise the egg."

"Yet it turns out they were still fully fertile."

Disheartened but not defeated, Brett and his colleagues looked to unravel this improbable twist.

"We started to dissect how a critical element could be eliminated and the sperm still retain its ability to initiate fertilisation," he comments.

"What we have since learnt is that there is likely a lot of redundancy in that initial interaction, so if one protein is removed from the equation, others are available to stand up and take its place."

"There must be a handful that participate, a reflection of the overall importance of this event in the initiation of a new life."

Mechanics and Mother Nature

Brett returned to Australia in 2001, signing on to work alongside Laureate Professor John Aitken at the University of Newcastle. "Too good and exciting an opportunity" to pass up, the past 14 years have been some of the Australian Research Council Future Fellow's most memorable – and productive.

"My team recently conducted a global analysis of the proteins present in fertile and infertile patients," he shares. 

"Collaborating with Dr Mark Baker, I came up with a small number of proteins that change consistently between the two."

"At the moment I'm focusing on just one, however."

"We think it's the master regulator that primes the cell for its interactions with the egg."

Faithful to his pastoral origins, Brett has also put in a grant to build up research on sperm-egg recognition in other species. Currently in the process of collecting pilot data for the project, the multitasking academic is specifically interested in applying ARTs to improve the quality of saltwater crocodiles bred at Rockhampton's Koorana Crocodile Farm.

"There is an inherent danger in putting a male crocodile with a female."

"They may fight, resulting in the loss of one or both animals."

"It would be a real advance for the industry if we could just house the latter as is now standard practice in other livestock industries."

Additionally concerned about conservation, Brett is also teaming up with Taronga Zoo on this project.

"We might be able to contribute to improving the success of captive breeding of crocodilian species, many of which are currently threatened or endangered," he suggests.

Asking the big questions

Brett is simultaneously endeavouring to understand why mammals have developed such a "complicated" fertilisation process. Fascinated from an "evolutionary point of view," he's particularly intrigued by the similarities and differences in sperm maturation events that happen in species such as platypus and echidnas versus that of higher mammals and other vertebrates.

"In our own species, sperm maturation takes place over several weeks!" he declares.

"The cells that leave the testes are functionally immature and do not gain the ability to engage in fertilisation until they travel through the male and female reproductive tracts."

"We don't yet know why this is and it is particularly curious given that in species such as the birds, sperm come out of the testes ready to go."

Always willing to lend a helping hand, Brett is not short of praise when it comes to talking about his predecessors and successors either.

"Laureate Professor John Aitken, Conjoint Professor Russell Jones and Professor Eileen McLaughlin have been inspiring mentors," he muses,

"They've pointed me in the right direction when things were wavering."

"I also count myself very fortunate to work with a wonderful group of students, research assistants, and academics."

"One of the most rewarding aspects of my job is the interaction I have with these individuals and watching my students develop research careers of their own."

Spot the difference

An investigator of indomitable spirit, Professor Brett Nixon is pursuing the mysteries of sperm dysfunction from multiple, linked perspectives

Read more

Career Summary

Biography

Research Expertise

I obtained my PhD from the University of Newcastle (awarded 1999) working on a collaborative project with the Vertebrate Biocontrol Cooperative Research Centre focused on characterising protein expression and function in male gametogenesis. This work helped to define to role of the male reproductive tract in post-testicular sperm maturation. I then undertook post-doctoral research in the Department of Cell Biology, Emory University, GA, USA (1999-2000). This work centred on the characterisation of knockout mouse models bearing targeted deletions of a key proteins involved in gamete interactions. In 2001, I was recruited to the University of Newcastle as an Associate Lecturer in Biological Sciences and promoted to the position of Lecturer in 2004, Senior Lecturer in 2007, Associate Professor in 2011, and Professor in 2016. Since appointment I have established an active research program focusing on the use of human and animal models to characterise the processes of gamete maturation. In spermatozoa, I am currently working on a number of key molecules that control cell function and regulate gamete interactions. In folliculogenesis, I have focused on cell signalling pathways that contribute to the initiation of primordial follicle activation and the effects of environmental ovotoxicants on these pathways. These projects are of strategic importance in the design of post-testicular methods of fertility regulation, the development of assisted conception systems for the preservation of endangered species and elucidation of the causes of human male infertility. My research niche complements existing programs in male germ cell development and ovarian biology within the Reproductive Science Group at the University of Newcastle. It also represents a strategically important subprogram of the University’s Priority Research Centre (PRC) for Reproductive Science.

Teaching Expertise
Undergraduate Teaching Since my appointment in 2001, I have made a significant and sustained contribution to the teaching effort of the Discipline of Biological Sciences through the delivery of lectures and supervision of practical classes and tutorials across a broad range of courses (Levels 1000 – 4000). I strive for excellence in teaching and generate consistently high SEC and SET evaluations and qualitative feedback. My current teaching commitment includes: BIOL1001 Cells, Molecules, and Organisms BIOL1001 Gifted and Talented Program (Course coordinator) BIOL2001 Molecular Laboratory Skills for Biological Sciences BIOL2010 Biochemistry (Course coordinator) BIOL2050 Molecular Genetics BIOL3200 Cellular Biotechnology BIOL3250 Biotechnology Placement BIOL4110 - BIOL4220 BSc (Biology) Honours / BBiotech Honours (Program coordinator) Research Training Since 2001, I have supervised 6 PhD, 1 MPhil and 13 Honours students to successful completion of their research higher degrees. I am currently the principal supervisor of 3 PhD and 1 Honours student and the co-supervisor of an additional 3 PhD students. My commitment to research training is evidenced by the recent award of RHD Supervisor of the Year (2009), the University of Newcastle’s highest accolade for student supervision. The quality of my students is attested by the award of numerous platform and poster prizes at national and international meetings, including: best student poster at Lorne Protein (2003), Oozoa award for best student oral presentation at the Society for Reproduction (SRB) annual meeting (2008 & 2010), best student poster at the Australian Society for Biochemistry and Molecular Biology annual meeting (2010), best student poster at the International Symposium on Spermatology (Japan, 2010), and the SRB - New Investigator award (2011).

Administrative Expertise
Service to the University I am dedicated to building achievements in relation to University, Community and Professional Service. Since appointment to my current position I have made a significant commitment to University Service and related professional activities. Specifically, I focused on initiatives that enrich the student learning experience and promote a vibrant research culture, thus contributing to the status of the University's teaching and research profile. My committee membership aligns with my recognised strengths in teaching and research, and has included: Assistant Dean Teaching and Learning, Faculty of Science and IT Student Recruitment Committee, Faculty of Science and IT Honours Committee Chair, School of Environmental and Life Sciences Postgraduate Committee, Discipline of Biological Sciences Analytical and Biomolecular Research Facility Advisory Committee OH&S Committee, Reproductive Sciences Group Service to the profession Elected member of Council / Chair of Awards Committee for Society for Reproductive Biology (2014 - ongoing) Member of Grant Review Panel for Reproductive Medicine, Obstetrics & Gynaecology, and Paediatrics, National Health and Medical Research Council of Australia (2013-ongoing) Associate Editor, Molecular Human Reproduction (ranked 3/28 in Reproductive Biology) (2011 - ongoing) I review for a number of grant bodies such as NHMRC and Medical Research Council (UK). I also review for 20 specialist journals including Reproduction, Biology of Reproduction, Human Reproduction, Developmental Biology (~20 manuscripts/year).

Collaborations
I have collaborative partnerships with a number of pre-eminent national and international scientists within the field of gamete biology. Similarly, I have established collaborations with IVF clinics to support the extension of my research program into the clinical domain. This nexus is critical for our ability to drive translational outcomes in understanding the aetiology of human male infertility.


Qualifications

  • PhD, University of Newcastle
  • Bachelor of Science (Honours), University of Newcastle
  • Bachelor of Science, University of Newcastle

Keywords

  • Andrology
  • Biology
  • Gamete Biology
  • Reproductive Biology

Fields of Research

Code Description Percentage
060899 Zoology not elsewhere classified 15
111499 Paediatrics and Reproductive Medicine not elsewhere classified 50
060199 Biochemistry and Cell Biology not elsewhere classified 35

Professional Experience

UON Appointment

Title Organisation / Department
Professor University of Newcastle
School of Environmental and Life Sciences
Australia

Academic appointment

Dates Title Organisation / Department
1/1/2015 -  Fellow ARC (Australian Research Council)
1/1/2013 -  Elected member of Council / Chair of Awards Committee Society for Reproductive Biology
Australia
1/1/2011 -  Editor - Molecular Human Reproduction Molecular Human Reproduction Journal
Australia
1/1/2008 -  Membership - Society for the Study of Reproduction Society for the Study of Reproduction
Australia
1/1/1997 -  Membership - Society for Reproductive Biology Society for Reproductive Biology
Australia
1/1/1999 - 1/12/2000 NIH Post-Doctoral Research Fellow Emory University, School of Medicine, Atlanta, GA
Department of Cell Biology
United States
1/7/2011 -  Associate Professor University of Newcastle
School of Environmental and Life Sciences
Australia
1/1/2008 - 1/6/2011 Senior Lecturer University of Newcastle
School of Environmental and Life Sciences
Australia
1/1/2004 - 1/1/2008 Lecturer University of Newcastle
School of Environmental and Life Sciences
Australia
1/1/2001 - 1/1/2004 Associate Lecturer University of Newcastle
School of Environmental and Life Sciences
Australia

Professional appointment

Dates Title Organisation / Department
1/1/2017 - 1/1/2021 Editorial Board Member

Editorial Board Member

Asian Journal of Andrology
China
1/1/2017 - 1/1/2021 Associate Editor

Associate Editor

Frontiers in Endocrinology (Specialty Section - Reproduction)
Switzerland
1/1/2016 - 31/12/2018 Co-chair Program Organising Committee

Co-chair Program Organising Committee

Society for Reproductive Biology
Australia

Awards

Award

Year Award
2019 Society for Reproductive Biology Presidents Lecturer
Society for Reproductive Biology

Distinction

Year Award
2015 Fellow of the Society for Reproductive Biology
Society for Reproductive Biology
2013 Vice-Chancellor’s Award for Teaching Excellence and Learning Support
University of Newcastle
2013 Vice-Chancellor’s citation for outstanding contributions to student learning
University of Newcastle
2009 Faculty of Science and IT Research Higher Degree Supervisor of the Year
Unknown
2009 The University of Newcastle Supervisor of the Year
Unknown

Prize

Year Award
2018 Excellent Editorial Board Member Award
Asian Journal of Andrology
2017 Outstanding Paper Award
Asian Journal of Andrology

Recipient

Year Award
2014 Citation for Outstanding Contributions to Student Learning
Office for Learning and Teaching

Recognition

Year Award
2011 Certificate of Recognition for excellent contribution to peer review
Unknown
1998 Finalist, Junior Scientist Competition
Unknown

Research Award

Year Award
2012 Award for Excellence in Reproductive Biology Research
Society for Reproductive Biology
2007 New Investigator Grant
Hunter Medical Research Institute
2006 Lalor Foundation Award
Unknown
1998 Student Travel Grant
Unknown

Invitations

Participant

Year Title / Rationale
2007 The Biology of Extracellular Molecular Chaperones
Organisation: Novartis Foundation
Edit

Publications

For publications that are currently unpublished or in-press, details are shown in italics.


Chapter (12 outputs)

Year Citation Altmetrics Link
2020 Nixon B, Bromfield E, 'New Horizons in Male Subfertility and Infertility', Male and Sperm Factors that Maximize IVF Success, Cambridge University Press, Cambridge, UK (2020)
Co-authors Elizabeth Bromfield
2019 Gould T, Jamaluddin M, Petit J, King SJ, Nixon B, Scott R, et al., 'Finding Needles in Haystacks: The Use of Quantitative Proteomics for the Early Detection of Colorectal Cancer', Advances in the Molecular Understanding of Colorectal Cancer, IntechOpen, Switzerland 1-32 (2019) [B1]
DOI 10.5772/intechopen.80942
Co-authors Peter Pockney, Rodney Scott, Muhammad Jamaluddin, Matt Dun
2018 Jones RC, Nixon B, Grützner F, 'Reproduction in Monotremes', Encyclopedia of Reproduction, Academic Press, New York City, New York, United States 602-608 (2018) [B1]
DOI 10.1016/B978-0-12-809633-8.20606-7
Citations Scopus - 1
2018 Nixon B, Bromfield EG, 'Sperm Capacitation', Reference Module in Biomedical Sciences. Encyclopedia of Reproduction, Academic Press, Amsterdam 272-278 (2018)
DOI 10.1016/B978-0-12-801238-3.64464-1
Citations Scopus - 1
Co-authors Elizabeth Bromfield
2017 Nixon B, Bromfield EG, Cui J, De Iuliis GN, 'Heat shock protein A2 (HSPA2): regulatory roles in germ cell development and sperm', The Role of Heat Shock Proteins in Reproductive System Development and Function, Springer International Publishing, Cham, Switzerland 67-93 (2017) [B1]
DOI 10.1007/978-3-319-51409-3_4
Citations Scopus - 14Web of Science - 11
Co-authors Geoffry DeiuliIs, Elizabeth Bromfield
2017 Nixon B, Dun MD, Aitken RJ, 'Proteomic Analysis of Human Spermatozoa', Immune Fertility: Impact of Immune Reactions on Human Fertility, Springer Nature, Switzerland 3-22 (2017) [B1]
DOI 10.1007/978-3-319-40788-3_1
Co-authors Matt Dun, John Aitken
2016 Holt JE, Stanger SJ, Nixon B, McLaughlin EA, 'Non-coding RNA in spermatogenesis and epididymal maturation', Advances in Experimental Medicine and Biology, Springer, Dordrecht 95-120 (2016) [B1]
DOI 10.1007/978-94-017-7417-8_6
Citations Scopus - 11Web of Science - 10
Co-authors Eileen Mclaughlin, Janet Bristow
2012 Redgrove KA, Aitken RJ, Nixon B, 'More than a simple lock and key mechanism: unraveling the intricacies of sperm-oocyte interactions', Binding Protein, InTech, Rijeka, Croatia 73-122 (2012) [B1]
DOI 10.5772/2897
Co-authors Kate Redgrove, John Aitken
2010 Aitken RJ, Baker MA, De Iuliis GN, Nixon B, 'New insights into sperm physiology and pathology', Fertility Control, Springer, Berlin 99-115 (2010) [B1]
Citations Scopus - 54
Co-authors Geoffry DeiuliIs, John Aitken, Mark Baker
2010 Dun MD, Mitchell LA, Aitken RJ, Nixon B, 'Sperm-zona pellucida interaction: Molecular mechanisms and the potential for contraceptive intervention', Fertility Control, Springer, Berlin 139-178 (2010) [B1]
Citations Scopus - 12
Co-authors John Aitken, Matt Dun
2009 Nixon B, Aitken RJ, 'Proteomics of human spermatozoa', Immune Infertility: The Impact of Immune Reactions on Human Infertility, Springer, Berlin 3-12 (2009) [B1]
DOI 10.1007/978-3-642-01379-9
Citations Scopus - 1
Co-authors John Aitken
2003 Jones RC, Clulow J, Kidd G, Chaturapanich G, Nixon B, Dacheux J-L, 'The avian epididymis: a simple conduit or a sophisticated adaptation?', The Third International Conference on the Epididymis, The Van Doren Co, Charlottesville, Virginia, USA 138-148 (2003)
Co-authors John Clulow
Show 9 more chapters

Journal article (135 outputs)

Year Citation Altmetrics Link
2020 Liu G, Baird AW, Parsons MJ, Fan K, Skerrett-Byrne DA, Nair PM, et al., 'Platelet activating factor receptor acts to limit colitis-induced liver inflammation', FASEB JOURNAL, 34 7718-7732 (2020) [C1]
DOI 10.1096/fj.201901779R
Citations Scopus - 1Web of Science - 1
Co-authors Bridie Goggins, Andrea Johns, Simon Keely, Philip Hansbro, David Skerrett-Byrne
2020 Nixon B, Cafe SL, Eamens AL, De Iuliis GN, Bromfield EG, Martin JH, et al., 'Molecular insights into the divergence and diversity of post-testicular maturation strategies', Molecular and Cellular Endocrinology, 517 110955-110955 (2020) [C1]
DOI 10.1016/j.mce.2020.110955
Co-authors Geoffry DeiuliIs, Elizabeth Bromfield, Matt Dun, Andy Eamens, David Skerrett-Byrne
2020 Walters JLH, Gadella BM, Sutherland JM, Nixon B, Bromfield EG, 'Male Infertility: Shining a Light on Lipids and Lipid-Modulating Enzymes in the Male Germline', Journal of Clinical Medicine, 9 (2020) [C1]
DOI 10.3390/jcm9020327
Co-authors Jessie Sutherland, Elizabeth Bromfield
2020 Cafe SL, Nixon B, Dun MD, Roman SD, Bernstein IR, Bromfield EG, 'Oxidative Stress Dysregulates Protein Homeostasis within the Male Germ Line', Antioxidants and Redox Signaling, 32 487-503 (2020) [C1]
DOI 10.1089/ars.2019.7832
Citations Scopus - 1Web of Science - 1
Co-authors Shaun Roman, Elizabeth Bromfield, Matt Dun
2020 Tamessar CT, Trigg NA, Nixon B, Skerrett-Byrne DA, Sharkey DJ, Robertson SA, et al., 'Roles of male reproductive tract extracellular vesicles in reproduction.', Am J Reprod Immunol, e13338 (2020)
DOI 10.1111/aji.13338
Co-authors David Skerrett-Byrne, Elizabeth Bromfield, John Schjenken
2020 Campbell L, Cafe SL, Upton R, Doody JS, Nixon B, Clulow J, Clulow S, 'A model protocol for the cryopreservation and recovery of motile lizard sperm using the phosphodiesterase inhibitor caffeine', CONSERVATION PHYSIOLOGY, 8 (2020) [C1]
DOI 10.1093/conphys/coaa044
Co-authors John Clulow, Rose Upton Uon, Simon Clulow
2020 Chu C, Yu L, Henry-Berger J, Ru Y-F, Kocer A, Champroux A, et al., 'Knockout of glutathione peroxidase 5 down-regulates the piRNAs in the caput epididymidis of aged mice.', Asian J Androl, (2020)
DOI 10.4103/aja.aja_3_20
Co-authors Andy Eamens
2020 Peters AE, Mihalas BP, Bromfield EG, Roman SD, Nixon B, Sutherland JM, 'Autophagy in Female Fertility: A Role in Oxidative Stress and Aging', ANTIOXIDANTS & REDOX SIGNALING, 32 550-568 (2020) [C1]
DOI 10.1089/ars.2019.7986
Citations Scopus - 4Web of Science - 2
Co-authors Shaun Roman, Jessie Sutherland, Elizabeth Bromfield
2020 Nowicka-bauer K, Nixon B, 'Molecular changes induced by oxidative stress that impair human sperm motility', Antioxidants, 9 (2020) [C1]
DOI 10.3390/antiox9020134
Citations Scopus - 4Web of Science - 5
2020 Mihalas BP, Redgrove KA, Bernstein IR, Robertson MJ, McCluskey A, Nixon B, et al., 'Dynamin 2-dependent endocytosis is essential for mouse oocyte development and fertility', FASEB Journal, 34 5162-5177 (2020) [C1]
DOI 10.1096/fj.201902184R
Co-authors Janet Bristow, Kate Redgrove, Adam Mccluskey, Jessie Sutherland, Eileen Mclaughlin
2020 Griffin RA, Swegen A, Baker M, Aitken RJ, Skerrett-Byrne DA, Silva Rodriguez A, et al., 'Mass spectrometry reveals distinct proteomic profiles in high- and low-quality stallion spermatozoa.', Reproduction, (2020)
DOI 10.1530/REP-20-0284
Co-authors Mark Baker, David Skerrett-Byrne, Zamira Gibb
2020 Dun MD, Mannan A, Rigby CJ, Butler S, Toop HD, Beck D, et al., 'Shwachman Bodian Diamond syndrome (SBDS) protein is a direct inhibitor of protein phosphatase 2A (PP2A) activity and overexpressed in acute myeloid leukaemia', Leukemia, (2020)
DOI 10.1038/s41375-020-0814-0
Co-authors David Skerrett-Byrne, Anoop Enjeti, Hubert Hondermarck, Nikki Verrills, Geoffry DeiuliIs, Ryan Duchatel, Sam Faulkner, Matt Dun, Heather Murray
2020 Aitken RJ, De Iuliis GN, Nixon B, 'The Sins of Our Forefathers: Paternal Impacts on De Novo Mutation Rate and Development.', Annu Rev Genet, (2020)
DOI 10.1146/annurev-genet-112618-043617
Co-authors Geoffry DeiuliIs, John Aitken
2020 Lord T, Nixon B, 'Metabolic Changes Accompanying Spermatogonial Stem Cell Differentiation', Developmental Cell, 52 399-411 (2020) [C1]
DOI 10.1016/j.devcel.2020.01.014
Citations Scopus - 1Web of Science - 2
Co-authors Tessa Lord
2019 Nixon B, De Iuliis GN, Dun MD, Zhou W, Trigg NA, Eamens AL, 'Profiling of epididymal small non-protein-coding RNAs', Andrology, 7 669-680 (2019) [C1]
DOI 10.1111/andr.12640
Citations Scopus - 1Web of Science - 1
Co-authors Matt Dun, Andy Eamens, Geoffry DeiuliIs
2019 Martin JH, Aitken RJ, Bromfield E, Cafe SL, Sutherland JM, Frost ER, et al., 'Investigation into the presence and functional significance of proinsulin C-peptide in the female germline', Biology of Reproduction, 100 1275-1289 (2019) [C1]
DOI 10.1093/biolre/ioz008
Co-authors John Aitken, Jessie Sutherland, Tessa Lord, Elizabeth Bromfield
2019 Nixon B, De Iuliis GN, Hart HM, Zhou W, Mathe A, Bernstein IR, et al., 'Proteomic profiling of mouse epididymosomes reveals their contributions to post-testicular sperm maturation', Molecular and Cellular Proteomics, 18 S91-S108 (2019) [C1]
DOI 10.1074/mcp.RA118.000946
Citations Scopus - 20Web of Science - 23
Co-authors Andrea Johns, Matt Dun, Muhammad Jamaluddin, David Skerrett-Byrne, Geoffry DeiuliIs, Elizabeth Bromfield
2019 Gaikwad AS, Anderson AL, Merriner DJ, O'Connor AE, Houston BJ, Aitken RJ, et al., 'GLIPR1L1 is an IZUMO-binding protein required for optimal fertilization in the mouse.', BMC Biology, 17 (2019) [C1]
DOI 10.1186/s12915-019-0701-1
Citations Scopus - 1Web of Science - 1
Co-authors John Aitken
2019 Houston BJ, Nixon B, McEwan KE, Martin J, King BV, Aitken RJ, De Iuliis GN, 'Whole-body exposures to radiofrequency-electromagnetic energy can cause DNA damage in mouse spermatozoa via an oxidative mechanism', Scientific Reports, 9 (2019) [C1]
DOI 10.1038/s41598-019-53983-9
Citations Scopus - 2Web of Science - 1
Co-authors John Aitken, Bruce King, Geoffry DeiuliIs
2019 Brown P, RELISH Consortium, Zhou Y, 'Large expert-curated database for benchmarking document similarity detection in biomedical literature search', Database, 2019 (2019) [C1]
DOI 10.1093/database/baz085
Citations Web of Science - 2
Co-authors Elizabeth Bromfield
2019 Duchatel RJ, Jackson ER, Alvaro F, Nixon B, Hondermarck H, Dun MD, 'Signal Transduction in Diffuse Intrinsic Pontine Glioma', PROTEOMICS, 19 (2019) [C1]
DOI 10.1002/pmic.201800479
Citations Scopus - 4Web of Science - 4
Co-authors Ryan Duchatel, Matt Dun, Hubert Hondermarck
2019 Nixon B, Johnston SD, Skerrett-Byrne DA, Anderson AL, Stanger SJ, Bromfield EG, et al., 'Modification of Crocodile Spermatozoa Refutes the Tenet That Post-testicular Sperm Maturation Is Restricted To Mammals', MOLECULAR & CELLULAR PROTEOMICS, 18 S59-S76 (2019) [C1]
DOI 10.1074/mcp.RA118.000904
Citations Scopus - 4Web of Science - 5
Co-authors David Skerrett-Byrne, Elizabeth Bromfield, Philip Hansbro, Matt Dun
2019 Nixon B, Bernstein IR, Cafe SL, Delehedde M, Sergeant N, Anderson AL, et al., 'A Kinase Anchor Protein 4 is vulnerable to oxidative adduction in male germ cells', Frontiers in Cell and Developmental Biology, 7 (2019) [C1]
DOI 10.3389/fcell.2019.00319
Citations Scopus - 1Web of Science - 2
Co-authors Geoffry DeiuliIs, Tessa Lord, Andy Eamens, Matt Dun, Elizabeth Bromfield
2019 Zhou W, Stanger SJ, Anderson AL, Bernstein IR, De Iuliis GN, McCluskey A, et al., 'Mechanisms of tethering and cargo transfer during epididymosome-sperm interactions.', BMC biology, 17 35-35 (2019) [C1]
DOI 10.1186/s12915-019-0653-5
Citations Scopus - 7Web of Science - 9
Co-authors Geoffry DeiuliIs, Adam Mccluskey, Matt Dun, Eileen Mclaughlin
2019 Bromfield E, Walters JLH, Cafe S, Bernstein I, Stanger SR, Anderson AL, et al., 'Differential cell death decisions in the testis: evidence for an exclusive window of ferroptosis in round spermatids', Molecular Human Reproduction, 25 241-256 (2019) [C1]
DOI 10.1093/molehr/gaz015
Citations Scopus - 5Web of Science - 6
Co-authors John Aitken, Matt Dun, Eileen Mclaughlin, Elizabeth Bromfield
2019 Xavier MJ, Roman SD, Aitken RJ, Nixon B, 'Transgenerational inheritance: how impacts to the epigenetic and genetic information of parents affect offspring health', HUMAN REPRODUCTION UPDATE, 25 519-541 (2019) [C1]
DOI 10.1093/humupd/dmz017
Citations Scopus - 16Web of Science - 13
Co-authors Shaun Roman, John Aitken
2019 Martin JH, Aitken RJ, Bromfield EG, Nixon B, 'DNA damage and repair in the female germline: contributions to ART.', Human Reproduction Update, 25 180-201 (2019) [C1]
DOI 10.1093/humupd/dmy040
Citations Scopus - 8Web of Science - 6
Co-authors Elizabeth Bromfield, John Aitken
2019 Mihalas BP, Camlin NJ, Xavier MJ, Peters AE, Holt JE, Sutherland JM, et al., 'The small non-coding RNA profile of mouse oocytes is modified during aging', AGING-US, 11 2968-2997 (2019) [C1]
DOI 10.18632/aging.101947
Citations Scopus - 1Web of Science - 1
Co-authors Janet Bristow, Eileen Mclaughlin, Andy Eamens, Jessie Sutherland
2019 Xavier MJ, Nixon B, Roman SD, Scott RJ, Drevet JR, Aitken RJ, 'Paternal impacts on development: Identification of genomic regions vulnerable to oxidative DNA damage in human spermatozoa', Human Reproduction, 34 1876-1890 (2019) [C1]

© 2019 The Author(s) 2019. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For permissions, pleas... [more]

© 2019 The Author(s) 2019. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com. STUDY QUESTION: Do all regions of the paternal genome within the gamete display equivalent vulnerability to oxidative DNA damage? SUMMARY ANSWER: Oxidative DNA damage is not randomly distributed in mature human spermatozoa but is instead targeted, with particular chromosomes being especially vulnerable to oxidative stress. WHAT IS KNOWN ALREADY: Oxidative DNA damage is frequently encountered in the spermatozoa of male infertility patients. Such lesions can influence the incidence of de novo mutations in children, yet it remains to be established whether all regions of the sperm genome display equivalent susceptibility to attack by reactive oxygen species. STUDY DESIGN, SIZE, DURATION: Human spermatozoa obtained from normozoospermic males (n = 8) were split into equivalent samples and subjected to either hydrogen peroxide (H2O2) treatment or vehicle controls before extraction of oxidized DNA using a modified DNA immunoprecipitation (MoDIP) protocol. Specific regions of the genome susceptible to oxidative damage were identified by next-generation sequencing and validated in the spermatozoa of normozoospermic males (n = 18) and in patients undergoing infertility evaluation (n = 14). PARTICIPANTS/MATERIALS, SETTING, METHODS: Human spermatozoa were obtained from normozoospermic males and divided into two identical samples prior to being incubated with either H2O2 (5 mm, 1 h) to elicit oxidative stress or an equal volume of vehicle (untreated controls). Alternatively, spermatozoa were obtained from fertility patients assessed as having high basal levels of oxidative stress within their spermatozoa. All semen samples were subjected to MoDIP to selectively isolate oxidized DNA, prior to sequencing of the resultant DNA fragments using a next-generation whole-genomic sequencing platform. Bioinformatic analysis was then employed to identify genomic regions vulnerable to oxidative damage, several of which were selected for real-time quantitative PCR (qPCR) validation. MAIN RESULTS AND THE ROLE OF CHANCE: Approximately 9000 genomic regions, 150-1000 bp in size, were identified as highly vulnerable to oxidative damage in human spermatozoa. Specific chromosomes showed differential susceptibility to damage, with chromosome 15 being particularly sensitive to oxidative attack while the sex chromosomes were protected. Susceptible regions generally lay outside protamine-and histone-packaged domains. Furthermore, we confirmed that these susceptible genomic sites experienced a dramatic (2-15-fold) increase in their burden of oxidative DNA damage in patients undergoing infertility evaluation compared to normal healthy donors. LIMITATIONS, REASONS FOR CAUTION: The limited number of samples analysed in this study warrants external validation, as do the implications of our findings. Selection of male fertility patients was based on high basal levels of oxidative stress within their spermatozoa as opposed to specific sub-classes of male factor infertility. WIDER IMPLICATIONS OF THE FINDINGS: The identification of genomic regions susceptible to oxidation in the male germ line will be of value in focusing future analyses into the mutational load carried by children in response to paternal factors such as age, the treatment of male infertility using ART and paternal exposure to environmental toxicants. STUDY FUNDING/COMPETING INTEREST(S): Project support was provided by the University of Newcastle's (UoN) Priority Research Centre for Reproductive Science. M.J.X. was a recipient of a UoN International Postgraduate Research Scholarship. B.N. is the recipient of a National Health and Medical Research Council of Australia Senior Research Fellowship. Authors declare no conflict of interest.

DOI 10.1093/humrep/dez153
Citations Scopus - 5Web of Science - 4
Co-authors John Aitken, Shaun Roman, Rodney Scott
2019 Trigg NA, Eamens AL, Nixon B, 'The contribution of epididymosomes to the sperm small RNA profile.', Reproduction (Cambridge, England), 157 R209-R223 (2019) [C1]
DOI 10.1530/rep-18-0480
Citations Scopus - 8Web of Science - 7
Co-authors Andy Eamens
2018 Zhou W, De Iuliis GN, Dun MD, Nixon B, 'Characteristics of the epididymal luminal environment responsible for sperm maturation and storage', Frontiers in Endocrinology, 9 (2018) [C1]
DOI 10.3389/fendo.2018.00059
Citations Scopus - 31Web of Science - 32
Co-authors Geoffry DeiuliIs, Matt Dun
2018 Wang TE, Li SH, Minabe S, Andreson AL, Dun MD, Maeda KI, et al., 'Mouse quiescin sulfhydryl oxidases exhibit distinct epididymal luminal distribution with segment-specific sperm surface associations', Biology of Reproduction, 99 1022-1033 (2018) [C1]
DOI 10.1093/biolre/ioy125
Citations Scopus - 3Web of Science - 4
Co-authors Matt Dun
2018 Xavier MJ, Nixon B, Roman SD, Aitken RJ, 'Improved methods of DNA extraction from human spermatozoa that mitigate experimentally-induced oxidative DNA damage', PLOS ONE, 13 (2018) [C1]
DOI 10.1371/journal.pone.0195003
Citations Scopus - 2Web of Science - 2
Co-authors John Aitken, Shaun Roman
2018 Zhou W, Sipilä P, De Iuliis G, Dun MD, Nixon B, 'Analysis of Epididymal Protein Synthesis and Secretion', Jove-Journal of Visualized Experiments, 138 (2018) [C1]
DOI 10.3791/58308
Citations Scopus - 5Web of Science - 3
Co-authors Geoffry DeiuliIs, Matt Dun
2018 Martin J, Bromfield EG, Aitken RJ, Lord T, Nixon B, 'Double Strand Break DNA Repair occurs via Non-Homologous End-Joining in Mouse MII Oocytes', Scientific Reports, 8 1-15 (2018) [C1]
DOI 10.1038/s41598-018-27892-2.
Citations Scopus - 7Web of Science - 7
Co-authors John Aitken, Tessa Lord, Elizabeth Bromfield
2018 Houston BJ, Nixon B, King BV, Aitken RJ, De Iuliis GN, 'Probing the Origins of 1,800 MHz Radio Frequency Electromagnetic Radiation Induced Damage in Mouse Immortalized Germ Cells and Spermatozoa in vitro', FRONTIERS IN PUBLIC HEALTH, 6 (2018) [C1]
DOI 10.3389/fpubh.2018.00270
Citations Web of Science - 5
Co-authors Bruce King, Geoffry DeiuliIs, John Aitken
2018 Walters JLH, De Iuliis G, Nixon B, Bromfield EG, 'Oxidative Stress in the Male Germline: A Review of Novel Strategies to Reduce 4-Hydroxynonenal Production', Antioxidants, 7 (2018) [C1]
DOI 10.3390/antiox7100132
Citations Scopus - 7Web of Science - 7
Co-authors Elizabeth Bromfield, Geoffry DeiuliIs
2018 Walters JLH, De Iuliis GN, Dun MD, Aitken RJ, McLaughlin EA, Nixon B, Bromfield EG, 'Pharmacological inhibition of arachidonate 15-lipoxygenase protects human spermatozoa against oxidative stress.', Biology of reproduction, 98 784-794 (2018) [C1]
DOI 10.1093/biolre/ioy058
Citations Scopus - 9Web of Science - 9
Co-authors John Aitken, Matt Dun, Elizabeth Bromfield, Eileen Mclaughlin, Geoffry DeiuliIs
2018 Mihalas BP, Bromfield EG, Sutherland JM, De Iuliis GN, McLaughlin EA, John Aitken R, Nixon B, 'Oxidative damage in naturally aged mouse oocytes is exacerbated by dysregulation of proteasomal activity', Journal of Biological Chemistry, 293 18944-18964 (2018) [C1]
DOI 10.1074/jbc.RA118.005751
Citations Scopus - 10Web of Science - 11
Co-authors Eileen Mclaughlin, Elizabeth Bromfield, Jessie Sutherland, John Aitken, Geoffry DeiuliIs
2018 Houston BJ, Nixon B, Martin JH, De Iuliis GN, Trigg NA, Bromfield EG, et al., 'Heat exposure induces oxidative stress and DNA damage in the male germ line', BIOLOGY OF REPRODUCTION, 98 593-606 (2018) [C1]
DOI 10.1093/biolre/ioy009
Citations Scopus - 27Web of Science - 25
Co-authors Geoffry DeiuliIs, John Aitken, Elizabeth Bromfield
2017 Zhou W, De Iuliis GN, Turner AP, Reid AT, Anderson AL, McCluskey A, et al., 'Developmental expression of the dynamin family of mechanoenzymes in the mouse epididymis', BIOLOGY OF REPRODUCTION, 96 159-173 (2017) [C1]
DOI 10.1095/biolreprod.116.145433
Citations Scopus - 8Web of Science - 7
Co-authors Geoffry DeiuliIs, Eileen Mclaughlin, Andrew Reid, Adam Mccluskey
2017 Bromfield EG, Aitken RJ, McLaughlin EA, Nixon B, 'Proteolytic degradation of heat shock protein A2 occurs in response to oxidative stress in male germ cells of the mouse', MOLECULAR HUMAN REPRODUCTION, 23 91-105 (2017) [C1]
DOI 10.1093/molehr/gaw074
Citations Scopus - 21Web of Science - 17
Co-authors John Aitken, Eileen Mclaughlin, Elizabeth Bromfield
2017 Hall SE, Nixon B, Aitken RJ, 'Non-surgical sterilisation methods may offer a sustainable solution to feral horse (Equus caballus) overpopulation', Reproduction, Fertility and Development, 29 1655-1666 (2017) [C1]
DOI 10.1071/RD16200
Citations Scopus - 7Web of Science - 5
Co-authors John Aitken
2017 Katen AL, Sipila P, Mitchell LA, Stanger SJ, Nixon B, Roman SD, 'Epididymal CYP2E1 plays a critical role in acrylamide-induced DNA damage in spermatozoa and paternally mediated embryonic resorptions', BIOLOGY OF REPRODUCTION, 96 921-935 (2017) [C1]
DOI 10.1093/biolre/iox021
Citations Scopus - 2Web of Science - 2
Co-authors Shaun Roman
2017 Bromfield EG, Mihalas BP, Dun MD, Aitken RJ, McLaughlin EA, Walters JLH, Nixon B, 'Inhibition of arachidonate 15-lipoxygenase prevents 4-hydroxynonenal-induced proteindamage in male germ cells', Biology of Reproduction, 96 598-609 (2017) [C1]
DOI 10.1093/biolre/iox005
Citations Scopus - 15Web of Science - 11
Co-authors Elizabeth Bromfield, Matt Dun, John Aitken, Eileen Mclaughlin
2017 Johnston SD, Qualischefski E, Cooper J, McLeod R, Lever J, Nixon B, et al., 'Cryopreservation of saltwater crocodile (Crocodylus porosus) spermatozoa', Reproduction, Fertility and Development, 29 2235-2244 (2017) [C1]
DOI 10.1071/RD16511
Citations Scopus - 3Web of Science - 3
2017 Mihalas BP, De Iuliis GN, Redgrove KA, McLaughlin EA, Nixon B, 'The lipid peroxidation product 4-hydroxynonenal contributes to oxidative stress-mediated deterioration of the ageing oocyte', SCIENTIFIC REPORTS, 7 (2017) [C1]
DOI 10.1038/s41598-017-06372-z
Citations Scopus - 28Web of Science - 29
Co-authors Kate Redgrove, Geoffry DeiuliIs, Eileen Mclaughlin
2017 Mihalas BP, Redgrove KA, McLaughlin EA, Nixon B, 'Molecular mechanisms responsible for increased vulnerability of the ageing oocyte to oxidative damage', Oxidative Medicine and Cellular Longevity, 2017 1-22 (2017) [C1]
DOI 10.1155/2017/4015874
Citations Scopus - 25Web of Science - 27
Co-authors Eileen Mclaughlin, Kate Redgrove
2017 Hutcheon K, McLaughlin EA, Stanger SJ, Bernstein IR, Dun MD, Eamens AL, Nixon B, 'Analysis of the small non-protein-coding RNA profile of mouse spermatozoa reveals specific enrichment of piRNAs within mature spermatozoa', RNA Biology, 14 1776-1790 (2017) [C1]

© 2017 Taylor & Francis Group, LLC. Post-testicular sperm maturation and storage within the epididymis is a key determinant of gamete quality and fertilization competence. H... [more]

© 2017 Taylor & Francis Group, LLC. Post-testicular sperm maturation and storage within the epididymis is a key determinant of gamete quality and fertilization competence. Here we demonstrate that mouse spermatozoa possess a complex small non-protein-coding RNA (sRNA) profile, the composition of which is markedly influenced by their epididymal transit. Thus, although microRNAs (miRNAs) are highly represented in the spermatozoa of the proximal epididymis, this sRNA class is largely diminished in mature spermatozoa of the distal epididymis. Coincident with this, a substantial enrichment in Piwi-interacting RNA (piRNA) abundance in cauda spermatozoa was detected. Further, features of cauda piRNAs, including; predominantly 29¿31 nts in length; preference for uracil at their 5' terminus; no adenine enrichment at piRNA nt 10, and; predominantly mapping to intergenic regions of the mouse genome, indicate that these piRNAs are generated by the PIWIL1-directed primary piRNA production pathway. Accordingly, PIWIL1 was detected via immunoblotting and mass spectrometry in epididymal spermatozoa. These data provide insight into the complexity and dynamic nature of the sRNA profile of spermatozoa and raise the intriguing prospect that piRNAs are generated in situ in maturing spermatozoa. Such information is of particular interest in view of the potential role for paternal sRNAs in influencing conception, embryo development and intergenerational inheritance.

DOI 10.1080/15476286.2017.1356569
Citations Scopus - 17Web of Science - 18
Co-authors Matt Dun, Eileen Mclaughlin, Andy Eamens
2017 Zhou W, Anderson AL, Turner AP, De Iuliis GN, McCluskey A, McLaughlin EA, Nixon B, 'Characterization of a novel role for the dynamin mechanoenzymes in the regulation of human sperm acrosomal exocytosis.', Molecular Human Reproduction, 23 657-673 (2017) [C1]
DOI 10.1093/molehr/gax044
Citations Scopus - 5Web of Science - 6
Co-authors Geoffry DeiuliIs, Eileen Mclaughlin, Adam Mccluskey
2017 Martin JH, Bromfield EG, Aitken RJ, Nixon B, 'Biochemical alterations in the oocyte in support of early embryonic development', CELLULAR AND MOLECULAR LIFE SCIENCES, 74 469-485 (2017) [C1]
DOI 10.1007/s00018-016-2356-1
Citations Scopus - 7Web of Science - 7
Co-authors Elizabeth Bromfield, John Aitken
2017 Hall SE, Aitken RJ, Nixon B, Smith ND, Gibb Z, 'Electrophilic aldehyde products of lipid peroxidation selectively adduct to heat shock protein 90 and arylsulfatase A in stallion spermatozoa', BIOLOGY OF REPRODUCTION, 96 107-121 (2017) [C1]
DOI 10.1095/biolreprod.116.145292
Citations Scopus - 7Web of Science - 7
Co-authors John Aitken, Zamira Gibb
2016 Redgrove KA, Bernstein IR, Pye VJ, Mihalas BP, Sutherland JM, Nixon B, et al., 'Dynamin 2 is essential for mammalian spermatogenesis', SCIENTIFIC REPORTS, 6 (2016) [C1]
DOI 10.1038/srep35084
Citations Scopus - 6Web of Science - 8
Co-authors Janet Bristow, Kate Redgrove, Jessie Sutherland, Eileen Mclaughlin, Adam Mccluskey
2016 Nixon B, Anderson AL, Smith ND, McLeod R, Johnston SD, 'The Australian saltwater crocodile (Crocodylus porosus) provides evidence that the capacitation of spermatozoa may extend beyond the mammalian lineage', Proceedings of the Royal Society B: Biological Sciences, 283 (2016) [C1]

© 2016 The Author(s) Published by the Royal Society. All rights reserved. Although mammalian spermatozoa only acquire functional maturity as they are conveyed through the male (ep... [more]

© 2016 The Author(s) Published by the Royal Society. All rights reserved. Although mammalian spermatozoa only acquire functional maturity as they are conveyed through the male (epididymal maturation) and female (capacitation) reproductive tracts, the degree of post-testicular development necessary to achieve fertilization in other vertebrate species remains far less clear. Indeed, despite reports that the epididymis of birds and reptiles is capable of secreting proteins that bind and modify the sperm surface characteristics, it remains unclear whether capacitation is a pre-requisite for fertilization in these species. Using the ancient reptilian Australian saltwater crocodile as a model, this study was undertaken to explore whether reptile sperm do undergo capacitation-like changes following ejaculation. Our studies revealed that crocodile spermatozoa experienced a rapid and sustained, cyclic-AMP mediated increase in progressive motility following incubation under conditions optimized for the induction of capacitation in mammalian species such as the mouse and human. This response was coupled with elevated levels of phosphorylation associated with both protein kinase A and tyrosine kinase substrates, the latter of which were predominantly localized within the sperm flagellum. In findings that also accord with mammalian spermatozoa, we confirmed a homologue of outer dense fibre 2 as one of the principal substrates for tyrosine phosphorylation. Overall, our findings support the concept that crocodile spermatozoa do undergo a process that is homologous to capacitation in preparation for fertilization of an ovum.

DOI 10.1098/rspb.2016.0495
Citations Scopus - 12Web of Science - 10
2016 Martin JH, Bromfield EG, Aitken RJ, Lord T, Nixon B, 'Data on the concentrations of etoposide, PSC833, BAPTA-AM, and cycloheximide that do not compromise the vitality of mature mouse oocytes, parthenogenetically activated and fertilized embryos', DATA IN BRIEF, 8 1215-1220 (2016)
DOI 10.1016/j.dib.2016.07.046
Citations Scopus - 4Web of Science - 4
Co-authors John Aitken, Elizabeth Bromfield, Tessa Lord
2016 Houston B, Nixon B, King BV, De Iuliis GN, Aitken RJ, 'The effects of radiofrequency electromagnetic radiation on sperm function', Reproduction, 152 R263-R276 (2016) [C1]
DOI 10.1530/REP-16-0126
Citations Scopus - 22Web of Science - 22
Co-authors Bruce King, John Aitken, Geoffry DeiuliIs
2016 Bromfield EG, McLaughlin EA, Aitken RJ, Nixon B, 'Heat shock protein member A2 forms a stable complex with angiotensin convertingenzymeand protein disulfide isomerase A6 in human spermatozoa', Molecular Human Reproduction, 22 93-109 (2016) [C1]
DOI 10.1093/molehr/gav073
Citations Scopus - 20Web of Science - 17
Co-authors Eileen Mclaughlin, Elizabeth Bromfield, John Aitken
2016 Martin JH, Nixon B, Lord T, Bromfield EG, Aitken RJ, 'Identification of a key role for permeability glycoprotein in enhancing the cellular defense mechanisms of fertilized oocytes', DEVELOPMENTAL BIOLOGY, 417 63-76 (2016) [C1]
DOI 10.1016/j.ydbio.2016.06.035
Citations Scopus - 13Web of Science - 11
Co-authors Tessa Lord, Elizabeth Bromfield, John Aitken
2016 Nixon B, Ecroyd H, Dacheux J-L, Dacheux F, Labas V, Johnston SD, Jones RC, 'Formation and Dissociation of Sperm Bundles in Monotremes', Biology of Reproduction, 95 1-11 (2016) [C1]
DOI 10.1095/biolreprod.116.140491
Citations Scopus - 10Web of Science - 7
2016 Stanger SJ, Law EA, Jamsai D, O'Bryan MK, Nixon B, McLaughlin EA, et al., 'A novel germ cell protein, SPIF (sperm PKA interacting factor), is essential for the formation of a PKA/TCP11 complex that undergoes conformational and phosphorylation changes upon capacitation', FASEB Journal, 30 2777-2791 (2016) [C1]

© 2016 FASEB. Spermatozoa require the process of capacitation to enable them to fertilize an egg. PKA is crucial to capacitation and the development of hyperactivated motility. Sp... [more]

© 2016 FASEB. Spermatozoa require the process of capacitation to enable them to fertilize an egg. PKA is crucial to capacitation and the development of hyperactivated motility. Sperm PKA is activated by cAMP generated by the germ cell-enriched adenylyl cyclase encoded by Adcy10. Male mice lacking Adcy10 are sterile, because their spermatozoa are immotile. The current studywas designed to identify binding partners of the sperm-specific (Ca2) catalytic subunit of PKA (PRKACA) by using it as the "bait" in a yeast 2-hybrid system. This approach was used to identify a novel germ cell-enriched protein, sperm PKA interacting factor (SPIF), in 25% of the positive clones. Homozygous Spif-nullmice were embryonically lethal. SPIF was coexpressed and coregulated with PRKACA and with t-complex protein (TCP)-11, a protein associated with PKA signaling. We established that these 3 proteins form part of a novel complex in mouse spermatozoa. Upon capacitation, the SPIF protein becomes tyrosine phosphorylatedin > 95% of sperm. Anapparent molecular rearrangement in the complex occurs, bringing PRKACA and TCP11 into proximity. Taken together, these results suggest a role for the novel complex of SPIF, PRKACA, and TCP11 during spermcapacitation, fertilization, and embryogenesis.

DOI 10.1096/fj.201500136R
Citations Scopus - 5Web of Science - 4
Co-authors Shaun Roman, Eileen Mclaughlin, John Aitken
2016 Aitken RJ, Muscio L, Whiting S, Connaughton HS, Fraser BA, Nixon B, et al., 'Analysis of the effects of polyphenols on human spermatozoa reveals unexpected impacts on mitochondrial membrane potential, oxidative stress and DNA integrity; implications for assisted reproductive technology', Biochemical Pharmacology, 121 78-96 (2016) [C1]
DOI 10.1016/j.bcp.2016.09.015
Citations Scopus - 12Web of Science - 10
Co-authors John Aitken, Geoffry DeiuliIs
2016 Reilly JN, McLaughlin EA, Stanger SJ, Anderson AL, Hutcheon K, Church K, et al., 'Characterisation of mouse epididymosomes reveals a complex profile of microRNAs and a potential mechanism for modification of the sperm epigenome', SCIENTIFIC REPORTS, 6 (2016) [C1]
DOI 10.1038/srep31794
Citations Scopus - 67Web of Science - 67
Co-authors Eileen Mclaughlin, Andy Eamens, Janet Bristow
2016 Katen AL, Chambers CG, Nixon B, Roman SD, 'Chronic Acrylamide Exposure in Male Mice Results in Elevated DNA Damage in the Germline and Heritable Induction of CYP2E1 in the Testes', BIOLOGY OF REPRODUCTION, 95 (2016) [C1]
DOI 10.1095/biolreprod.116.139535
Citations Scopus - 10Web of Science - 9
Co-authors Shaun Roman
2016 Katen AL, Stanger SJ, Anderson AL, Nixon B, Roman SD, 'Chronic acrylamide exposure in male mice induces DNA damage to spermatozoa; Potential for amelioration by resveratrol', REPRODUCTIVE TOXICOLOGY, 63 1-12 (2016) [C1]
DOI 10.1016/j.reprotox.2016.05.004
Citations Scopus - 17Web of Science - 17
Co-authors Shaun Roman
2015 Anderson AL, Stanger SJ, Mihalas BP, Tyagi S, Holt JE, McLaughlin EA, Nixon B, 'Assessment of microRNA expression in mouse epididymal epithelial cells and spermatozoa by next generation sequencing', Genomics Data, 6 208-211 (2015) [C1]

© 2015 Elsevier Inc. The mammalian epididymis is a highly specialized region of the male reproductive tract that is lined with a continuous layer of epithelial cells that display ... [more]

© 2015 Elsevier Inc. The mammalian epididymis is a highly specialized region of the male reproductive tract that is lined with a continuous layer of epithelial cells that display a remarkable level of regionalized secretory and absorptive activity. The luminal environment created by this combined secretory and absorptive activity is directly responsible for promoting the functional maturation of spermatozoa and their maintenance in a quiescent and viable state prior to ejaculation. This study was designed to identify the complement of microRNAs (miRNAs) that are expressed within the mouse epididymal epithelial cells and the maturing populations of spermatozoa. Through the use of Next Generation Sequencing technology we have demonstrated that both epididymal epithelial cells and spermatozoa harbour a complex repertoire of miRNAs that have substantially different expression profiles along the length of the tract. These data, deposited in the Gene Expression Omnibus (GEO) with the accession numbers GSE70197 and GSE70198, afford valuable insight into the post-transcriptional control of gene expression within the epididymis and provide the first evidence for the dynamic transformation of the miRNA content of maturing sperm cells. Ultimately such information promises to inform our understanding of the aetiology of male infertility. Herein we provide a detailed description of the methodology used to generate these important data.

DOI 10.1016/j.gdata.2015.09.012
Citations Scopus - 14Web of Science - 12
Co-authors Janet Bristow, Eileen Mclaughlin
2015 Bromfield E, Aitken RJ, Nixon B, 'Novel characterization of the HSPA2-stabilizing protein BAG6 in human spermatozoa', Molecular Human Reproduction, 21 755-769 (2015) [C1]

© The Author 2015. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. While a large cohort of sperm ... [more]

© The Author 2015. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. While a large cohort of sperm surface receptors underpin sperm-oocyte adhesion processes, our recent work has revealed that the molecular chaperone Heat Shock Protein A2 (HSPA2) is a key regulator of zona pellucida-receptor complex assembly in our own species. Indeed, in the infertile population, spermatozoa that fail to interact with the zona pellucida of the oocyte consistently lack HSPA2 protein expression. While the mechanisms behind this protein deficiency are under consideration, BCL2-associated athanogene 6 (BAG6) has been identified as a key regulator of HSPA2 stability in mouse germ cells. However, in the human, the presence of BAG family proteins remains completely uncharacterized. Consequently, this study aimed to determine the presence of BAG6 in human sperm cells and to characterize its putative interaction with HSPA2 throughout sperm cell development. BAG6 was shown to co-localize with HSPA2 in human testicular germ cells and epididymal spermatozoa. Similarly, BAG6 was identified in the equatorial region of non-capacitated spermatozoa but underwent a marked relocation to the anterior region of the head upon the induction of capacitation in these cells. Protein-protein interaction assays revealed the stable interaction of BAG6 and HSPA2 proteins in mature spermatozoa. Furthermore, examination of the spermatozoa of infertile men with zona pellucida binding defects, related to a lack of HSPA2, revealed a concomitant deficiency in BAG6 protein expression. In view of the findings described in this study, we propose that BAG6 is likely a key regulator of HSPA2 stability/function in human germ cells. Moreover, its under-representation in spermatozoa with zona pellucida binding deficiency suggests that BAG6 may be an important candidate to study for a further understanding of male idiopathic infertility.

DOI 10.1093/molehr/gav041
Citations Scopus - 25Web of Science - 19
Co-authors John Aitken, Elizabeth Bromfield
2015 Nixon B, Stanger SJ, Mihalas BP, Reilly JN, Anderson AL, Dun MD, et al., 'Next generation sequencing analysis reveals segmental patterns of microRNA expression in mouse epididymal epithelial cells', PLoS ONE, 10 (2015) [C1]

© 2015 Nixon et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and repro... [more]

© 2015 Nixon et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. The functional maturation of mammalian spermatozoa is accomplished as the cells descend through the highly specialized microenvironment of the epididymis. This dynamic environment is, in turn, created by the combined secretory and absorptive activity of the surrounding epithelium and displays an extraordinary level of regionalization. Although the regulatory network responsible for spatial coordination of epididymal function remains unclear, recent evidence has highlighted a novel role for the RNA interference pathway. Indeed, as noncanonical regulators of gene expression, small noncoding RNAs have emerged as key elements of the circuitry involved in regulating epididymal function and hence sperm maturation. Herein we have employed next generation sequencing technology to profile the genome-wide miRNA signatures of mouse epididymal cells and characterize segmental patterns of expression. An impressive profile of some 370 miRNAs were detected in the mouse epididymis, with a subset of these specifically identified within the epithelial cells that line the tubule (218). A majority of the latter miRNAs (75%) were detected at equivalent levels along the entire length of the mouse epididymis. We did however identify a small cohort of miRNAs that displayed highly regionalized patterns of expression, including miR-204-5p and miR-196b-5p, which were down- and up-regulated by approximately 39- and 45-fold between the caput/caudal regions, respectively. In addition we identified 79 miRNAs (representing ~ 21% of all miRNAs) as displaying conserved expression within all regions of the mouse, rat and human epididymal tissue. These included 8/14 members of let-7 family of miRNAs that have been widely implicated in the control of androgen signaling and the repression of cell proliferation and oncogenic pathways. Overall these data provide novel insights into the sophistication of the miRNA network that regulates the function of the male reproductive tract.

DOI 10.1371/journal.pone.0135605
Citations Scopus - 29Web of Science - 26
Co-authors Matt Dun, Eileen Mclaughlin, Janet Bristow
2015 Aitken RJ, Cummins JM, Nixon B, 'The 12 (th) International Symposium on Spermatology.', Asian J Androl, 17 519-520 (2015) [C3]
DOI 10.4103/1008-682X.153852
Co-authors John Aitken
2015 Aitken RJ, Baker MA, Nixon B, 'Are sperm capacitation and apoptosis the opposite ends of a continuum driven by oxidative stress?', Asian J Androl, 17 633-639 (2015) [C1]
DOI 10.4103/1008-682X.153850
Citations Scopus - 79Web of Science - 76
Co-authors John Aitken, Mark Baker
2015 Nixon B, Stanger SJ, Mihalas BP, Reilly JN, Anderson AL, Tyagi S, et al., 'The MicroRNA Signature of Mouse Spermatozoa Is Substantially Modified During Epididymal Maturation', Biology of Reproduction, 93 (2015) [C1]

© 2015 by the Society for the Study of Reproduction, Inc. In recent years considerable effort has been devoted to understanding the epigenetic control of sperm development, leadin... [more]

© 2015 by the Society for the Study of Reproduction, Inc. In recent years considerable effort has been devoted to understanding the epigenetic control of sperm development, leading to an increased appreciation of the importance of RNA interference pathways, and in particular miRNAs, as key regulators of spermatogenesis and epididymal maturation. It has also been shown that sperm are endowed with an impressive array of miRNA that have been implicated in various aspects of fertilization and embryo development. However, to date there have been no reports on whether the sperm miRNA signature is static or whether it is influenced by their prolonged maturation within the male reproductive tract. To investigate this phenomenon, we employed next-generation sequencing to systematically profile the miRNA signature of maturing mouse spermatozoa. In so doing we have provided the first evidence for the posttesticular modification of the sperm miRNA profile under normal physiological conditions. Such modifications include the apparent loss and acquisition of an impressive cohort of some 113 and 115 miRNAs, respectively, between the proximal and distal epididymal segments. Interestingly, the majority of these changes occur late in maturation and include the uptake of novel miRNA species in addition to a significant increase in many miRNAs natively expressed in immature sperm. Because sperm are not capable of de novo transcription, these findings identify the epididymis as an important site in establishing the sperm epigenome with the potential to influence the peri-conceptual environment of the female reproductive tract, contribute to the inheritance of acquired characteristics, and/or alter the developmental trajectory of the resulting offspring.

DOI 10.1095/biolreprod.115.132209
Citations Scopus - 71Web of Science - 71
Co-authors Eileen Mclaughlin, Janet Bristow
2015 Reid AT, Anderson AL, Roman SD, McLaughlin EA, McCluskey A, Robinson PJ, et al., 'Glycogen synthase kinase 3 regulates acrosomal exocytosis in mouse spermatozoa via dynamin phosphorylation', FASEB JOURNAL, 29 2872-2882 (2015) [C1]
DOI 10.1096/fj.14-265553
Citations Scopus - 13Web of Science - 12
Co-authors John Aitken, Adam Mccluskey, Shaun Roman, Andrew Reid, Eileen Mclaughlin
2015 Bromfield EG, Aitken RJ, Anderson AL, McLaughlin EA, Nixon B, 'The impact of oxidative stress on chaperone-mediated human sperm-egg interaction.', Hum Reprod, 30 2597-2613 (2015) [C1]
DOI 10.1093/humrep/dev214
Citations Scopus - 48Web of Science - 45
Co-authors Elizabeth Bromfield, John Aitken, Eileen Mclaughlin
2015 Nixon B, Bromfield EG, Dun MD, Redgrove KA, McLaughlin EA, Aitken RJ, 'The role of the molecular chaperone heat shock protein A2 (HSPA2) in regulating human sperm-egg recognition', ASIAN JOURNAL OF ANDROLOGY, 17 568-573 (2015) [C1]
DOI 10.4103/1008-682X.151395
Citations Scopus - 36Web of Science - 30
Co-authors Matt Dun, Eileen Mclaughlin, Elizabeth Bromfield, John Aitken, Kate Redgrove
2014 Nixon BJ, Katen AL, Stanger SJ, Schjenken JE, Nixon B, Roman SD, 'Mouse spermatocytes express CYP2E1 and respond to acrylamide exposure', PLoS ONE, 9 (2014) [C1]

Metabolism of xenobiotics by cytochrome P450s (encoded by the CYP genes) often leads to bio-activation, producing reactive metabolites that interfere with cellular processes and c... [more]

Metabolism of xenobiotics by cytochrome P450s (encoded by the CYP genes) often leads to bio-activation, producing reactive metabolites that interfere with cellular processes and cause DNA damage. In the testes, DNA damage induced by xenobiotics has been associated with impaired spermatogenesis and adverse effects on reproductive health. We previously reported that chronic exposure to the reproductive toxicant, acrylamide, produced high levels of DNA damage in spermatocytes of Swiss mice. CYP2E1 metabolises acrylamide to glycidamide, which, unlike acrylamide, readily forms adducts with DNA. Thus, to investigate the mechanisms of acrylamide toxicity in mouse male germ cells, we examined the expression of the CYP, CYP2E1, which metabolises acrylamide. Using Q-PCR and immunohistochemistry, we establish that CYP2E1 is expressed in germ cells, in particular in spermatocytes. Additionally, CYP2E1 gene expression was upregulated in these cells following in vitro acrylamide exposure (1 µM, 18 h). Spermatocytes were isolated and treated with 1 µM acrylamide or 0.5 µM glycidamide for 18 hours and the presence of DNA-adducts was investigated using the comet assay, modified to detect DNA-adducts. Both compounds produced significant levels of DNA damage in spermatocytes, with a greater response observed following glycidamide exposure. A modified comet assay indicated that direct adduction of DNA by glycidamide was a major source of DNA damage. Oxidative stress played a small role in eliciting this damage, as a relatively modest effect was found in a comet assay modified to detect oxidative adducts following glycidamide exposure, and glutathione levels remained unchanged following treatment with either compound. Our results indicate that the male germ line has the capacity to respond to xenobiotic exposure by inducing detoxifying enzymes, and the DNA damage elicited by acrylamide in male germ cells is likely due to the formation of glycidamide adducts. © 2014 Nixon et al.

DOI 10.1371/journal.pone.0094904
Citations Scopus - 19Web of Science - 17
Co-authors Shaun Roman, John Schjenken
2014 Bromfield EG, Aitken RJ, Gibb Z, Lambourne SR, Nixon B, 'Capacitation in the presence of methyl-beta-cyclodextrin results in enhanced zona pellucida-binding ability of stallion spermatozoa', REPRODUCTION, 147 153-166 (2014) [C1]
DOI 10.1530/REP-13-0393
Citations Scopus - 31Web of Science - 27
Co-authors John Aitken, Zamira Gibb, Elizabeth Bromfield
2014 Nixon B, Ewen KA, Krivanek KM, Clulow J, Kidd G, Ecroyd H, Jones RC, 'Post-testicular sperm maturation and identification of an epididymal protein in the Japanese quail (Coturnix coturnix japonica)', REPRODUCTION, 147 265-277 (2014) [C1]
DOI 10.1530/REP-13-0566
Citations Scopus - 17Web of Science - 16
Co-authors John Clulow
2013 McIver SC, Roman SD, Nixon B, Loveland KL, McLaughlin EA, 'The rise of testicular germ cell tumours: The search for causes, risk factors and novel therapeutic targets', F1000 Research, 2 1-11 (2013) [C1]
DOI 10.12688/f1000research.2-55.v2
Co-authors Shaun Roman, Eileen Mclaughlin
2013 Lord T, Nixon B, Jones KT, Aitken RJ, 'Melatonin Prevents Postovulatory Oocyte Aging in the Mouse and Extends the Window for Optimal Fertilization In Vitro', Biology of Reproduction, 88 1-9 (2013) [C1]
DOI 10.1095/biolreprod.112.106450
Citations Scopus - 81Web of Science - 81
Co-authors John Aitken, Tessa Lord
2013 Nixon BJ, Stanger SJ, Nixon B, Roman SD, 'Erratum to Chronic Exposure to Acrylamide Induces DNA Damage in Male Germ Cells of Mice [Toxicological Sciences, 129, 1 (2012) 135-145] doi:10.1093/toxsci/kfs178', Toxicological Sciences, 132 250 (2013)
DOI 10.1093/toxsci/kfs311
Citations Scopus - 1Web of Science - 1
Co-authors Shaun Roman
2013 Redgrove KA, Anderson AL, McLaughlin EA, O'Bryan MK, Aitken RJ, Nixon B, 'Investigation of the mechanisms by which the molecular chaperone HSPA2 regulates the expression of sperm surface receptors involved in human spermoocyte recognition', MOLECULAR HUMAN REPRODUCTION, 19 120-135 (2013) [C1]
DOI 10.1093/molehr/gas064
Citations Scopus - 51Web of Science - 44
Co-authors Eileen Mclaughlin, John Aitken, Kate Redgrove
2013 Aitken RJ, Nixon B, 'Sperm capacitation: a distant landscape glimpsed but unexplored', Molecular Human Reproduction, 19 785-793 (2013) [C1]
DOI 10.1093/molehr/gat067
Citations Scopus - 101Web of Science - 94
Co-authors John Aitken
2013 Bromfield EG, Nixon B, 'The function of chaperone proteins in the assemblage of protein complexes involved in gamete adhesion and fusion processes', Reproduction, 145 R31-R42 (2013) [C1]
DOI 10.1530/REP-12-0316
Citations Scopus - 31Web of Science - 27
Co-authors Elizabeth Bromfield
2013 McIver SC, Loveland KL, Roman SD, Nixon B, Kitazawa R, McLaughlin EA, 'The chemokine CXCL12 and its receptor CXCR4 are implicated in human seminoma metastasis', ANDROLOGY, 1 517-529 (2013) [C1]
DOI 10.1111/j.2047-2927.2013.00081.x
Citations Scopus - 25Web of Science - 25
Co-authors Shaun Roman, Eileen Mclaughlin
2012 Reid AT, Lord T, Stanger SJ, Roman SD, McCluskey A, Robinson PJ, et al., 'Dynamin regulates specific membrane fusion events necessary for acrosomal exocytosis in mouse spermatozoa', Journal of Biological Chemistry, 287 37659-37672 (2012) [C1]
Citations Scopus - 36Web of Science - 35
Co-authors Andrew Reid, John Aitken, Adam Mccluskey, Shaun Roman, Tessa Lord
2012 Sutherland JM, Keightley RA, Nixon B, Roman SD, Robker RL, Russell DL, McLaughlin EA, 'Suppressor of cytokine signaling 4 (SOCS4): Moderator of ovarian primordial follicle activation', Journal of Cellular Physiology, 227 1188-1198 (2012) [C1]
Citations Scopus - 20Web of Science - 22
Co-authors Jessie Sutherland, Shaun Roman, Eileen Mclaughlin
2012 Sobinoff AP, Pye VJ, Nixon B, Roman SD, McLaughlin EA, 'Jumping the gun: Smoking constituent BaP causes premature primordial follicle activation and impairs oocyte fusibility through oxidative stress', Toxicology and Applied Pharmacology, 260 70-80 (2012) [C1]
Citations Scopus - 54Web of Science - 51
Co-authors Shaun Roman, Eileen Mclaughlin
2012 Dun MD, Anderson AL, Bromfield EG, Asquith KL, Emmett BJ, McLaughlin EA, et al., 'Investigation of the expression and functional significance of the novel mouse sperm protein, a disintegrin and metalloprotease with thrombospondin type 1 motifs number 10 (ADAMTS10)', International Journal of Andrology, 35 572-589 (2012) [C1]
DOI 10.1111/j.1365-2605.2011.01235.x
Citations Scopus - 21Web of Science - 17
Co-authors Matt Dun, Eileen Mclaughlin, Kelly Asquith, John Aitken, Elizabeth Bromfield
2012 Sobinoff AP, Nixon B, Roman SD, McLaughlin EA, 'Staying alive: PI3K pathway promotes primordial follicle activation and survival in response to 3MC-induced ovotoxicity', Toxicological Sciences, 128 258-271 (2012) [C1]
Citations Scopus - 43Web of Science - 42
Co-authors Shaun Roman, Eileen Mclaughlin
2012 Nixon BJ, Stanger SJ, Nixon B, Roman SD, 'Chronic exposure to acrylamide induces DNA damage in male germ cells of mice', Toxicological Sciences, 129 135-145 (2012) [C1]
Citations Scopus - 31Web of Science - 28
Co-authors Shaun Roman
2012 McIver SC, Roman SD, Nixon B, McLaughlin EA, 'miRNA and mammalian male germ cells', Human Reproduction Update, 18 44-59 (2012) [C1]
Citations Scopus - 91Web of Science - 86
Co-authors Shaun Roman, Eileen Mclaughlin
2012 Dun MD, Aitken RJ, Nixon B, 'The role of molecular chaperones in spermatogenesis and the post-testicular maturation of mammalian spermatozoa', Human Reproduction Update, 18 420-435 (2012) [C1]
DOI 10.1093/humupd/dms009
Citations Scopus - 57Web of Science - 53
Co-authors John Aitken, Matt Dun
2012 McIver SC, Stanger SJ, Santarelli DMF, Roman SD, Nixon B, McLaughlin EA, 'A unique combination of male germ cell mirnas coordinates gonocyte differentiation', PLoS One, 7 1-14 (2012) [C1]
Citations Scopus - 56Web of Science - 55
Co-authors Shaun Roman, Eileen Mclaughlin
2012 Redgrove KA, Nixon B, Baker MA, Hetherington L, Baker G, Liu D-Y, Aitken RJ, 'The molecular chaperone HSPA2 plays a key role in regulating the expression of sperm surface receptors that mediate sperm-egg recognition', Plos One, 7 1-16 (2012) [C1]
Citations Scopus - 79Web of Science - 69
Co-authors John Aitken, Kate Redgrove, Mark Baker
2012 Baker MA, Nixon B, Naumovski N, Aitken RJ, 'Proteomic insights into the maturation and capacitation of mammalian spermatozoa', Systems Biology in Reproductive Medicine, 58 211-217 (2012) [C1]
DOI 10.3109/19396368.2011.639844
Citations Scopus - 60Web of Science - 54
Co-authors Mark Baker, John Aitken
2011 Redgrove KA, Anderson AL, Dun MD, McLaughlin EA, O'Bryan MK, Aitken RJ, Nixon B, 'Involvement of multimeric protein complexes in mediating the capacitation-dependent binding of human spermatozoa to homologous zonae pellucidae', Developmental Biology, 356 460-474 (2011) [C1]
DOI 10.1016/j.ydbio.2011.05.674
Citations Scopus - 65Web of Science - 64
Co-authors Kate Redgrove, Matt Dun, Eileen Mclaughlin, John Aitken
2011 Dun MD, Smith ND, Baker MA, Lin M, Aitken RJ, Nixon B, 'The chaperonin containing TCP1 complex (CCT/TRiC) is involved in mediating sperm-oocyte interaction', Journal of Biological Chemistry, 286 36875-36887 (2011) [C1]
DOI 10.1074/jbc.m110.188888
Citations Scopus - 71Web of Science - 62
Co-authors Matt Dun, Mark Baker, John Aitken
2011 Nixon B, Mitchell LA, Anderson AL, McLaughlin EA, O'Bryan MK, Aitken RJ, 'Proteomic and functional analysis of human sperm detergent resistant membranes', Journal of Cellular Physiology, 226 2651-2665 (2011) [C1]
DOI 10.1002/jcp.22615
Citations Scopus - 65Web of Science - 63
Co-authors Eileen Mclaughlin, John Aitken
2011 Ainsworth CJ, Nixon B, Aitken RJ, 'The electrophoretic separation of spermatozoa: An analysis of genotype, surface carbohydrate composition and potential for capacitation', International Journal of Andrology, 34 e422-e434 (2011) [C1]
DOI 10.1111/j.1365-2605.2011.01164.x
Citations Scopus - 19Web of Science - 15
Co-authors John Aitken
2011 Nixon B, Ecroyd HW, Dacheux J-L, Jones RC, 'Monotremes provide a key to understanding the evolutionary significance of epididymal sperm maturation', Journal of Andrology, 32 665-671 (2011) [C1]
DOI 10.2164/jandrol.110.012716
Citations Scopus - 10Web of Science - 10
2011 Sobinoff AP, Mahony M, Nixon B, Roman SD, McLaughlin EA, 'Understanding the villain: DMBA-induced preantral ovotoxicity involves selective follicular destruction and primordial follicle activation through PI3K/Akt and mTOR signaling', Toxicological Sciences, 123 563-575 (2011) [C1]
Citations Scopus - 40Web of Science - 40
Co-authors Eileen Mclaughlin, Shaun Roman
2010 Gibbs GM, Lo JCY, Nixon B, Jamsai D, O'Connor AE, Rijal S, et al., 'Glioma pathogenesis-related 1-Like 1 is testis enriched, dynamically modified, and redistributed during male germ cell maturation and has a potential role in sperm-oocyte binding', Endocrinology, 151 2331-2342 (2010) [C1]
DOI 10.1210/en.2009-1255
Citations Scopus - 27Web of Science - 26
2010 Reid AT, Redgrove KA, Aitken RJ, Nixon B, 'Cellular mechanisms regulating sperm-zona pellucida interaction', Asian Journal of Andrology, 13 88-96 (2010) [C1]
DOI 10.1038/aja.2010.74
Citations Scopus - 46Web of Science - 42
Co-authors John Aitken, Kate Redgrove, Andrew Reid
2010 Sobinoff AP, Pye VJ, Nixon B, Roman SD, McLaughlin EA, 'Adding insult to injury: Effects of xenobiotic-induced preantral ovotoxicity on ovarian development and oocyte fusibility', Toxicological Sciences, 118 653-666 (2010) [C1]
DOI 10.1093/toxsci/kfq272
Citations Scopus - 38Web of Science - 40
Co-authors Eileen Mclaughlin, Shaun Roman
2010 Nixon B, Bielanowicz AJ, Anderson AL, Walsh AK, Hall TE, McCloghry AK, Aitken RJ, 'Elucidation of the signaling pathways that underpin capacitation-associated surface phosphotyrosine expression in mouse spermatozoa', Journal of Cellular Physiology, 224 71-83 (2010) [C1]
DOI 10.1002/jcp.22090
Citations Scopus - 31Web of Science - 27
Co-authors John Aitken
2009 Bennetts LE, De Iuliis GN, Nixon B, Kime M, Zelski K, McVicar CM, et al., 'Impact of Estrogenic Compounds on DNA Integrity in Human Spermatozoa: Evidence for Cross-Linking and Redox Cycling Activities', JOURNAL OF UROLOGY, 181 914-915 (2009) [C1]
Citations Scopus - 72Web of Science - 70
Co-authors Geoffry DeiuliIs, John Aitken
2009 De Iuliis GN, Thomson LK, Mitchell LA, Read JM, Koppers AJ, Hedges A, et al., 'DNA damage in human spermatozoa is highly correlated with the efficiency of chromatin remodeling and the formation of 8-hydroxy-2'-deoxyguanosine, a marker of oxidative stress', Biology of Reproduction, 81 517-524 (2009) [C1]
DOI 10.1095/biolreprod.109.076836
Citations Scopus - 223Web of Science - 226
Co-authors Geoffry DeiuliIs, John Aitken
2009 Nixon B, Bielanowicz AJ, McLaughlin EA, Tanphaichitr N, Ensslin MA, Aitken RJ, 'Composition and significance of detergent resistant membranes in mouse spermatozoa', Journal of Cellular Physiology, 218 122-134 (2009) [C1]
DOI 10.1002/jcp.21575
Citations Scopus - 87Web of Science - 83
Co-authors Eileen Mclaughlin, John Aitken
2009 Nixon B, Aitken RJ, 'The biological significance of detergent-resistant membranes in spermatozoa', Journal of Reproductive Immunology, 83 8-13 (2009) [C1]
DOI 10.1016/j.jri.2009.06.258
Citations Scopus - 42Web of Science - 36
Co-authors John Aitken
2009 Dacheux J-L, Dacheux F, Labas V, Ecroyd H, Nixon B, Jones RC, 'New proteins identified in epididymal fluid from the platypus (Ornithorhynchus anatinus)', Reproduction Fertility and Development, 21 1002-1007 (2009) [C1]
DOI 10.1071/rd09091
Citations Scopus - 11Web of Science - 11
2009 Ecroyd H, Nixon B, Dacheux J-L, Jones RC, 'Testicular descent, sperm maturation and capacitation: Lessons from our most distant relatives, the monotremes', Reproduction Fertility and Development, 21 992-1001 (2009) [C1]
DOI 10.1071/rd09081
Citations Scopus - 8Web of Science - 6
2008 Mitchell LA, Nixon B, Baker MA, Aitken RJ, 'Investigation of the role of SRC in capacitation-associated tyrosine phosphorylation of human spermatozoa', Molecular Human Reproduction, 14 235-243 (2008) [C1]
DOI 10.1093/molehr/gan007
Citations Scopus - 61Web of Science - 60
Co-authors Mark Baker, John Aitken
2008 Grutzner F, Nixon B, Jones RC, 'Reproductive biology in egg-laying mammals', Sexual Development, 2 115-127 (2008) [C1]
DOI 10.1159/000143429
Citations Scopus - 18Web of Science - 14
2008 Nixon B, Jones RC, Holland MK, 'Molecular and functional characterization of the rabbit epididymal secretory protein 52, REP52', Biology of Reproduction, 78 910-920 (2008) [C1]
DOI 10.1095/biolreprod.107.065524
Citations Scopus - 7Web of Science - 7
2008 Walsh AK, Whelan D, Bielanowicz AJ, Skinner BL, Aitken RJ, O'Bryan MK, Nixon B, 'Identification of the molecular chaperone, heat shock protein 1 (chaperonin 10), in the reproductive tract and in capacitating spermatozoa in the male mouse', Biology of Reproduction, 78 983-993 (2008) [C1]
DOI 10.1095/biolreprod.107.066860
Citations Scopus - 35Web of Science - 32
Co-authors John Aitken
2008 Warren WC, Hillier LW, Graves JAM, Birney E, Ponting CP, Grutzner F, et al., 'Genome analysis of the platypus reveals unique signatures of evolution (vol 453, pg 175, 2008)', NATURE, 455 256-256 (2008) [C3]
DOI 10.1038/nature07253
Citations Scopus - 7Web of Science - 8
2008 Warren WC, Hillier LW, Graves JAM, Birney E, Ponting CP, Grutzner F, et al., 'Genome analysis of the platypus reveals unique signatures of evolution', Nature, 453 175-U1 (2008) [C1]
DOI 10.1038/nature06936
Citations Scopus - 489Web of Science - 479
2008 Priestley MN, Dunstan RH, O'Connor WA, Van Zwieten L, Nixon B, Macfarlane GR, 'Effects of 4-nonylphenol and 17 alpha-ethynylestradiol exposure in the Sydney rock oyster, Saccostrea glomerata: Vitellogenin induction and gonadal development', Aquatic Toxicology, 88 39-47 (2008) [C1]
DOI 10.1016/j.aquatox.2008.03.003
Citations Scopus - 58Web of Science - 51
Co-authors Geoff Macfarlane, Hugh Dunstan
2007 Aitken RJ, Nixon B, Lin M, Koppers AJ, Lee YH, Baker MA, 'Proteomic changes in mammalian spermatozoa during epididymal maturation', Asian Journal of Andrology, 9 554-564 (2007) [C1]
DOI 10.1111/j.1745-7262.2007.00280.x
Citations Scopus - 121Web of Science - 109
Co-authors John Aitken, Mark Baker
2007 Jones RC, Dacheux JL, Nixon B, Ecroyd HW, 'Role of the epididymis in sperm competition', Asian Journal of Andrology, 9 493-499 (2007) [C1]
DOI 10.1111/j.1745-7262.2007.00284.x
Citations Scopus - 27Web of Science - 26
2007 Mitchell LA, Nixon B, Aitken RJ, 'Analysis of chaperone proteins associated with human spermatozoa during capacitation', Molecular Human Reproduction, 13 605-613 (2007) [C1]
DOI 10.1093/molehr/gam043
Citations Scopus - 57Web of Science - 54
Co-authors John Aitken
2007 Nixon B, Aitken RJ, McLaughlin EA, 'New insights into the molecular mechanisms of sperm-egg interaction', Cellular and Molecular Life Sciences, 64 1805-1823 (2007) [C1]
DOI 10.1007/s00018-007-6552-x
Co-authors Eileen Mclaughlin, John Aitken
2007 Ainsworth CJ, Nixon B, Jansen RPS, Aitken RJ, 'First recorded pregnancy and normal birth after ICSI using electrophoretically isolated spermatozoa', Human Reproduction, 22 197-200 (2007) [C1]
DOI 10.1093/humrep/del351
Citations Scopus - 62Web of Science - 53
Co-authors John Aitken
2006 Nixon B, Macintyre DA, Mitchell LA, Gibbs GM, O'Bryan M, Aitken RJ, 'The identification of mouse sperm-surface-associated proteins and characterization of their ability to act as decapacitation factors', Biology of Reproduction, 74 275-287 (2006) [C1]
DOI 10.1095/biolreprod.105.044644
Citations Scopus - 102Web of Science - 101
Co-authors John Aitken
2005 Nixon B, Paul JW, Spiller CM, Attwell-Heap AG, Ashman LK, Aitken RJ, 'Evidence for the involvement of PECAM-1 in a receptor mediated signal-transduction pathway regulating capacitation-associated tyrosine phosphorylation in human spermatozoa', Journal of Cell Science, 118 4865-4877 (2005) [C1]
DOI 10.1242/jcs.02604
Citations Scopus - 15Web of Science - 16
Co-authors Jonathan Paul, Leonie Ashman, John Aitken
2005 Nixon B, Asquith KL, Aitken RJ, 'The role of molecular chaperones in mouse sperm-egg interactions', Molecular and Cellular Endocrinology, 240 1-10 (2005) [C1]
DOI 10.1016/j.mce.2005.06.004
Citations Scopus - 25Web of Science - 21
Co-authors Kelly Asquith, John Aitken
2005 Ainsworth CJ, Nixon B, Aitken RJ, 'Development of a novel electrophoretic system for the isolation of human spermatozoa', Human Reproduction, 20 2261-2270 (2005) [C1]
DOI 10.1093/humrep/dei024
Citations Scopus - 115Web of Science - 98
Co-authors John Aitken
2005 Asquith KL, Bielanowicz AJ, McLaughlin EA, Nixon B, Aitken RJ, 'Localization and significance of molecular chaperones, heat shock protein 1, and tumor rejection antigen gp96 in the male reproductive tract and during capacitation and acrosome reaction', Biology of Reproduction, 72 328-337 (2005) [C1]
DOI 10.1095/biolreprod.104.034470
Citations Scopus - 81Web of Science - 69
Co-authors Eileen Mclaughlin, Kelly Asquith, John Aitken
2004 Asquith KL, Baleato R, McLaughlin EA, Nixon B, Aitken RJ, 'Tyrosine phosphorylation activates surface chaperones facilitating sperm-zona recognition', Journal of Cell Science, 117 3645-3657 (2004) [C1]
DOI 10.1242/jcs.01214
Citations Scopus - 148Web of Science - 133
Co-authors Kelly Asquith, Eileen Mclaughlin, John Aitken
2004 Curry BJ, Su H, Law EG, McLaughlin EA, Nixon B, Aitken RJ, 'Identification of RARhoGAP, a novel putative RhoGAP gene expressed in male germ cells', Genomics, 84 406-418 (2004) [C1]
DOI 10.1016/j.ygeno.2004.03.004
Citations Scopus - 9Web of Science - 10
Co-authors Ben Curry, Eileen Mclaughlin, John Aitken
2002 Nixon B, Jones RC, Hansen LA, Holland M, 'Rabbit epididymal secretory proteins. I. Characterization and hormonal regulation', Biology of Reproduction, 67 133-139 (2002) [C1]
Citations Scopus - 13Web of Science - 11
2002 Nixon B, Jones RC, Clarke H, Holland M, 'Rabbit epididymal secretory proteins. II. Immunolocalization and sperm association of REP38', Biology of Reproduction, 67 140-146 (2002) [C1]
Citations Scopus - 6Web of Science - 5
2002 Nixon B, Hardy C, Jones RC, Anfrews J, Holland M, 'Rabbit epididymal secretory proteins. III. Molecular cloning and characterization of the complementary DNA for REP38', Biology of Reproduction, 67 147-153 (2002) [C1]
Citations Scopus - 5Web of Science - 4
2001 Nixon B, Lu QX, Wassler MJ, Foote CI, Ensslin MA, Shur BD, 'Galactosyltransferase function during mammalian fertilization', CELLS TISSUES ORGANS, 168 46-57 (2001) [C1]
DOI 10.1159/000016805
Citations Scopus - 60Web of Science - 59
1998 Holland MK, Nixon B, 'The specificity of epididymal secretory proteins', Journal of Reproduction and Fertility, 197-210 (1998)
1997 Hardy C, Clarke H, Nixon B, Hinds L, Holland M, 'Examination of the immunocontraceptive potential of recombinant fertilin subunits in rabbits', Biology of Reproduction, 57 879-886 (1997) [C1]
Citations Scopus - 39Web of Science - 35
Show 132 more journal articles

Review (2 outputs)

Year Citation Altmetrics Link
2013 McIver SM, Roman SD, Nixon B, Loveland KL, McLaughlin EA, 'The rise of testicular germ cell tumours: the search for causes, risk factors and novel therapeutic targets', F1000Research (2013)
Citations Scopus - 11
Co-authors Eileen Mclaughlin, Shaun Roman
2007 Nixon B, Aitken RJ, McLaughlin EA, 'New insights into the molecular mechanisms of sperm-egg interaction', CELLULAR AND MOLECULAR LIFE SCIENCES (2007) [D1]
DOI 10.1007/s00018-007-6552-x
Citations Scopus - 80Web of Science - 75
Co-authors John Aitken, Eileen Mclaughlin
Edit

Grants and Funding

Summary

Number of grants 64
Total funding $13,823,113

Click on a grant title below to expand the full details for that specific grant.


20203 grants / $2,312,686

Utilising male fertility as a biomarker of health to understand the biological effects of PFAS$1,319,338

Funding body: NHMRC (National Health & Medical Research Council)

Funding body NHMRC (National Health & Medical Research Council)
Project Team Professor Brett Nixon, Doctor Brett Turner, Doctor Brett Turner, Doctor Geoffry De Iuliis, Doctor Geoffry De Iuliis, Dr Bradley Clarke, Doctor Shaun Roman, Doctor Shaun Roman, Doctor Matt Dun, Doctor Matt Dun, Doctor Andy Eamens, Doctor Andy Eamens, Dr Mark Green, Dr Mark Green, Dr Bradley Clarke
Scheme Targeted Call for Research - Per and Poly-Fluoroalkylated Substances (PFAS)
Role Lead
Funding Start 2020
Funding Finish 2023
GNo G1900626
Type Of Funding C1100 - Aust Competitive - NHMRC
Category 1100
UON Y

Investigating the role of hypoxic niche microenvironments and hypoxia-induced transcription factors in regulating spermatogonial stem cell function$567,590

Funding body: NHMRC (National Health & Medical Research Council)

Funding body NHMRC (National Health & Medical Research Council)
Project Team Doctor Tessa Lord, Professor Brett Nixon
Scheme Ideas Grants
Role Investigator
Funding Start 2020
Funding Finish 2022
GNo G1900404
Type Of Funding Aust Competitive - Commonwealth
Category 1CS
UON Y

MRSP 2020 Pregnancy and Reproduction Program$425,758

Funding body: Hunter Medical Research Institute

Funding body Hunter Medical Research Institute
Project Team Laureate Professor Roger Smith, Laureate Professor John Aitken, Professor Craig Pennell, Professor Lee Smith, Professor Jon Hirst, Professor Brett Nixon, Associate Professor Kirsty Pringle, Doctor Jonathan Paul, Doctor Jessie Sutherland
Scheme NSW MRSP Infrastructure Grant
Role Investigator
Funding Start 2020
Funding Finish 2020
GNo G1901541
Type Of Funding C2220 - Aust StateTerritoryLocal - Other
Category 2220
UON Y

20194 grants / $1,701,739

Mechanistic and translational studies in male reproductive health$666,292

Funding body: NHMRC (National Health & Medical Research Council)

Funding body NHMRC (National Health & Medical Research Council)
Project Team Professor Brett Nixon
Scheme Research Fellowships
Role Lead
Funding Start 2019
Funding Finish 2023
GNo G1800012
Type Of Funding Aust Competitive - Commonwealth
Category 1CS
UON Y

Targeted disruption of lipoxygenase enzymes to prevent oxidative stress-mediated pathologies in the male germline$586,586

Funding body: NHMRC (National Health & Medical Research Council)

Funding body NHMRC (National Health & Medical Research Council)
Project Team Professor Brett Nixon, Doctor Elizabeth Bromfield, Laureate Professor John Aitken
Scheme Project Grant
Role Lead
Funding Start 2019
Funding Finish 2021
GNo G1800389
Type Of Funding Aust Competitive - Commonwealth
Category 1CS
UON Y

Utilising the male germline to define our understanding of the biological effects of radio frequency electromagnetic energy$435,307

Funding body: NHMRC (National Health & Medical Research Council)

Funding body NHMRC (National Health & Medical Research Council)
Project Team Doctor Geoffry De Iuliis, Dr King Yuk Chan, chan, King Yuk, Professor Brett Nixon, Professor Rodica Ramer, Ramer, Rodica
Scheme Project Grant
Role Investigator
Funding Start 2019
Funding Finish 2021
GNo G1800050
Type Of Funding Aust Competitive - Commonwealth
Category 1CS
UON Y

Research Consultancy$13,554

Funding body: Kazia Laboratories Pty Limited

Funding body Kazia Laboratories Pty Limited
Project Team Doctor Elizabeth Bromfield, Professor Brett Nixon
Scheme Research Consultancy
Role Investigator
Funding Start 2019
Funding Finish 2019
GNo G1900374
Type Of Funding C3111 - Aust For profit
Category 3111
UON Y

20183 grants / $2,235,745

Accumulation, Storage, and Release of Sperm in the Oviduct$1,612,829

Accumulation, Storage, and Release of Sperm in the Oviduct

Funding body: National Institutes of Health

Funding body National Institutes of Health
Project Team

David Miller, Brett Nixon, Randy Prather, Nicolai Bovin

Scheme MRS Research Grant
Role Investigator
Funding Start 2018
Funding Finish 2022
GNo
Type Of Funding International - Competitive
Category 3IFA
UON N

Elucidating the role of epididymosomes in the transfer of fertility-modulating proteins and regulatory classes of RNA to maturing spermatozoa$547,155

Funding body: NHMRC (National Health & Medical Research Council)

Funding body NHMRC (National Health & Medical Research Council)
Project Team Professor Brett Nixon, Doctor Matt Dun
Scheme Project Grant
Role Lead
Funding Start 2018
Funding Finish 2020
GNo G1700434
Type Of Funding Aust Competitive - Commonwealth
Category 1CS
UON Y

Capillary Flow Two Dimensional High Pressure Liquid Chromatography (HPLC) system$75,761

Funding body: NHMRC (National Health & Medical Research Council)

Funding body NHMRC (National Health & Medical Research Council)
Project Team Professor Brett Nixon, Doctor Matt Dun, Associate Professor Nikki Verrills, Professor Hubert Hondermarck, Associate Professor Mark Baker, Doctor Elizabeth Bromfield
Scheme Equipment Grant
Role Lead
Funding Start 2018
Funding Finish 2018
GNo G1800470
Type Of Funding Scheme excluded from IGS
Category EXCL
UON Y

20174 grants / $23,400

Jennie Thomas Medical Research Travel Grant$8,900

Funding body: Hunter Medical Research Institute

Funding body Hunter Medical Research Institute
Project Team Miss Jacinta Martin, Professor Brett Nixon, Laureate Professor John Aitken, Doctor Elizabeth Bromfield
Scheme Jennie Thomas Medical Research Travel Grant
Role Lead
Funding Start 2017
Funding Finish 2017
GNo G1701521
Type Of Funding C3120 - Aust Philanthropy
Category 3120
UON Y

Jennie Thomas Travel Award$7,000

Funding body: Hunter Medical Research Institute

Funding body Hunter Medical Research Institute
Project Team Miss Bettina Mihalas, Professor Brett Nixon, Professor Eileen McLaughlin
Scheme Jennie Thomas Medical Research Travel Grant
Role Lead
Funding Start 2017
Funding Finish 2017
GNo G1701509
Type Of Funding C3120 - Aust Philanthropy
Category 3120
UON Y

Fertilisation Induced Changes to Oocyte Protective Machinery: Investigating Chemotherapeutics $5,000

Funding body: Hunter Medical Research Institute

Funding body Hunter Medical Research Institute
Project Team Professor Brett Nixon, Laureate Professor John Aitken, Doctor Elizabeth Bromfield, Miss Jacinta Martin
Scheme Greaves Family Postgraduate Top Up Scholarship in Medical Research
Role Lead
Funding Start 2017
Funding Finish 2017
GNo G1700388
Type Of Funding C3120 - Aust Philanthropy
Category 3120
UON Y

Effects of acrylamide on the male germline$2,500

Funding body: Hunter Medical Research Institute

Funding body Hunter Medical Research Institute
Project Team Doctor Shaun Roman, Professor Brett Nixon, Miss Caitlin Chambers
Scheme HMRI Student Association 'Future' Postgraduate Medical Research Scholarship
Role Investigator
Funding Start 2017
Funding Finish 2017
GNo G1700585
Type Of Funding C3120 - Aust Philanthropy
Category 3120
UON Y

20164 grants / $1,123,370

The role of dynamin in spermatogenesis, sperm maturation and sperm-oocyte interactions$567,478

Funding body: NHMRC (National Health & Medical Research Council)

Funding body NHMRC (National Health & Medical Research Council)
Project Team Professor Brett Nixon, Professor Eileen McLaughlin
Scheme Project Grant
Role Lead
Funding Start 2016
Funding Finish 2018
GNo G1500178
Type Of Funding Aust Competitive - Commonwealth
Category 1CS
UON Y

Investigation of the mechanisms underpinning HSPA2 dysfunction in the spermatozoa of infertile patients$494,958

Funding body: NHMRC (National Health & Medical Research Council)

Funding body NHMRC (National Health & Medical Research Council)
Project Team Professor Brett Nixon, Laureate Professor John Aitken, Professor Eileen McLaughlin
Scheme Project Grant
Role Lead
Funding Start 2016
Funding Finish 2018
GNo G1500165
Type Of Funding Aust Competitive - Commonwealth
Category 1CS
UON Y

Beckman Coulter Optima Max-TL, Benchtop Ultra Centrifuge, TLA-110 Fixed-Angle Rotor Package, TLS-55 Swinging Bucket Rotor Package$40,934

Funding body: NHMRC (National Health & Medical Research Council)

Funding body NHMRC (National Health & Medical Research Council)
Project Team Professor Brett Nixon, Laureate Professor John Aitken, Professor Eileen McLaughlin, Associate Professor Mark Baker, Doctor Jessie Sutherland, Doctor Elizabeth Bromfield
Scheme Equipment Grant
Role Lead
Funding Start 2016
Funding Finish 2016
GNo G1601314
Type Of Funding C2120 - Aust Commonwealth - Other
Category 2120
UON Y

Emlyn and Jennie Thomas Postgraduate Medical Research Scholarship$20,000

Funding body: Hunter Medical Research Institute

Funding body Hunter Medical Research Institute
Project Team Miss Bettina Mihalas, Professor Brett Nixon, Professor Eileen McLaughlin
Scheme Postgraduate Research Scholarship
Role Lead
Funding Start 2016
Funding Finish 2017
GNo G1601053
Type Of Funding C3120 - Aust Philanthropy
Category 3120
UON Y

20151 grants / $417,343

Use of quinone adducted peptides in the regulation of fertility$417,343

Funding body: ARC (Australian Research Council)

Funding body ARC (Australian Research Council)
Project Team Laureate Professor John Aitken, Professor Brett Nixon
Scheme Discovery Projects
Role Investigator
Funding Start 2015
Funding Finish 2017
GNo G1400318
Type Of Funding Aust Competitive - Commonwealth
Category 1CS
UON Y

20148 grants / $1,490,388

Investigation of novel mechanisms for the regulation of sperm-oocyte interactions$901,056

Funding body: ARC (Australian Research Council)

Funding body ARC (Australian Research Council)
Project Team Professor Brett Nixon
Scheme Future Fellowships
Role Lead
Funding Start 2014
Funding Finish 2018
GNo G1301259
Type Of Funding Aust Competitive - Commonwealth
Category 1CS
UON Y

miRNA regulation of sperm maturation $396,157

Funding body: NHMRC (National Health & Medical Research Council)

Funding body NHMRC (National Health & Medical Research Council)
Project Team Professor Brett Nixon, Professor Eileen McLaughlin, Doctor Janet Bristow
Scheme Project Grant
Role Lead
Funding Start 2014
Funding Finish 2016
GNo G1300125
Type Of Funding Aust Competitive - Commonwealth
Category 1CS
UON Y

JuLI Stage $71,674

Funding body: NHMRC (National Health & Medical Research Council)

DVC(R) Research Support for Future Fellow (FT14)$59,501

Funding body: University of Newcastle

Funding body University of Newcastle
Project Team Professor Brett Nixon
Scheme Future Fellowship Support
Role Lead
Funding Start 2014
Funding Finish 2018
GNo G1400948
Type Of Funding Internal
Category INTE
UON Y

The Role of the GTPase Dynamin in the Female Germline$30,000

Funding body: Hunter Medical Research Institute

Funding body Hunter Medical Research Institute
Project Team Doctor Kate Redgrove, Doctor Janet Bristow, Professor Eileen McLaughlin, Professor Brett Nixon
Scheme Bridging Grant
Role Investigator
Funding Start 2014
Funding Finish 2014
GNo G1301333
Type Of Funding Grant - Aust Non Government
Category 3AFG
UON Y

The role of dynamin is spermatogenesis and sperm-oocyte interactions$20,000

Funding body: University of Newcastle

Funding body University of Newcastle
Project Team Professor Brett Nixon, Professor Eileen McLaughlin, Professor Adam McCluskey
Scheme Near Miss Grant
Role Lead
Funding Start 2014
Funding Finish 2014
GNo G1301400
Type Of Funding Internal
Category INTE
UON Y

Development of contraception on demand $10,000

Funding body: University of Newcastle

Funding body University of Newcastle
Project Team Professor Brett Nixon, Laureate Professor John Aitken
Scheme Near Miss Grant
Role Lead
Funding Start 2014
Funding Finish 2014
GNo G1301399
Type Of Funding Internal
Category INTE
UON Y

Faculty PVC Conference Assistance Grant 2014$2,000

Funding body: University of Newcastle - Faculty of Science & IT

Funding body University of Newcastle - Faculty of Science & IT
Project Team Professor Brett Nixon
Scheme PVC Conference Assistance Grant
Role Lead
Funding Start 2014
Funding Finish 2014
GNo G1401230
Type Of Funding Internal
Category INTE
UON Y

20136 grants / $381,182

Novel function of heat shock protein 2A in the regulation of human sperm-egg interactions$292,182

Funding body: NHMRC (National Health & Medical Research Council)

Funding body NHMRC (National Health & Medical Research Council)
Project Team Professor Brett Nixon, Laureate Professor John Aitken, Professor Eileen McLaughlin
Scheme Project Grant
Role Lead
Funding Start 2013
Funding Finish 2015
GNo G1200193
Type Of Funding Aust Competitive - Commonwealth
Category 1CS
UON Y

Novel mechanisms for the regulation of sperm-oocyte interactions$20,000

Funding body: University of Newcastle

Funding body University of Newcastle
Project Team Professor Brett Nixon
Scheme Near Miss Grant
Role Lead
Funding Start 2013
Funding Finish 2013
GNo G1300458
Type Of Funding Internal
Category INTE
UON Y

NucleoCounter SP100$14,000

Funding body: NHMRC (National Health & Medical Research Council)

Funding body NHMRC (National Health & Medical Research Council)
Project Team Laureate Professor John Aitken, Professor Brett Nixon, Doctor Shaun Roman, Associate Professor Mark Baker, Doctor Geoffry De Iuliis, Doctor Lisa Mitchell, Doctor Matthew Jobling, Doctor Zamira Gibb, Mr Nenad Naumovski
Scheme Equipment Grant
Role Investigator
Funding Start 2013
Funding Finish 2013
GNo G1201175
Type Of Funding Other Public Sector - Commonwealth
Category 2OPC
UON Y

Dynamin - role in sperm oocyte interaction$10,000

Funding body: University of Newcastle

Funding body University of Newcastle
Project Team Professor Eileen McLaughlin, Professor Brett Nixon, Professor Adam McCluskey
Scheme Near Miss Grant
Role Investigator
Funding Start 2013
Funding Finish 2013
GNo G1300483
Type Of Funding Internal
Category INTE
UON Y

Dynamin - role in sperm oocyte interaction$10,000

Funding body: Hunter Medical Research Institute

Funding body Hunter Medical Research Institute
Project Team Professor Eileen McLaughlin, Professor Brett Nixon, Professor Adam McCluskey
Scheme Near Miss
Role Investigator
Funding Start 2013
Funding Finish 2013
GNo G1300700
Type Of Funding C3112 - Aust Not for profit
Category 3112
UON Y

20122 grants / $17,447

The role of miRNAs in the regulation of sperm maturation$10,000

Funding body: University of Newcastle - Faculty of Science & IT

Funding body University of Newcastle - Faculty of Science & IT
Project Team Professor Brett Nixon, Professor Eileen McLaughlin, Doctor Natalie Beveridge
Scheme Strategic Initiative Research Fund (SIRF)
Role Lead
Funding Start 2012
Funding Finish 2012
GNo G1401028
Type Of Funding Internal
Category INTE
UON Y

Faculty Visiting Fellowship 2012$7,447

Funding body: University of Newcastle - Faculty of Science & IT

Funding body University of Newcastle - Faculty of Science & IT
Project Team Professor Brett Nixon
Scheme Visiting Fellowship
Role Lead
Funding Start 2012
Funding Finish 2012
GNo G1401130
Type Of Funding Internal
Category INTE
UON Y

20112 grants / $40,000

SLX4 - a key regulator of male germ cell development and DNA repair$25,000

Funding body: Hunter Medical Research Institute

Funding body Hunter Medical Research Institute
Project Team Professor Eileen McLaughlin, Professor Brett Nixon
Scheme Project Grant
Role Investigator
Funding Start 2011
Funding Finish 2011
GNo G1001000
Type Of Funding Contract - Aust Non Government
Category 3AFC
UON Y

Eppendorf mastercycler pro with thermomixer comfort and 5430R centrifuge$15,000

Funding body: NHMRC (National Health & Medical Research Council)

Funding body NHMRC (National Health & Medical Research Council)
Project Team Professor Eileen McLaughlin, Professor Peter Lewis, Professor Adam McCluskey, Conjoint Professor Keith Jones, Professor Brett Nixon, Doctor Shaun Roman, Doctor Jennette Sakoff, Associate Professor Ian Grainge, Doctor Janet Bristow, Doctor Xiao Yang
Scheme Equipment Grant
Role Investigator
Funding Start 2011
Funding Finish 2011
GNo G1100028
Type Of Funding Other Public Sector - Commonwealth
Category 2OPC
UON Y

20107 grants / $1,695,000

An Integrated LC-MS-NMR facility for Applications in Proteomics and Organic Chemistry$500,000

Funding body: ARC (Australian Research Council)

Funding body ARC (Australian Research Council)
Project Team Professor Adam McCluskey, Laureate Professor John Aitken, Professor Paul Dastoor, Professor Phillip Robinson, Professor Eileen McLaughlin, Emeritus Professor Geoffrey Lawrance, Emeritus Professor Marcel Maeder, Professor Hugh Dunstan, Doctor Shaun Roman, Conjoint Professor Rob Atkin, Associate Professor Clovia Holdsworth, Associate Professor Mark Baker, Associate Professor Nikki Verrills, Professor Gottfried Otting, Professor Brett Nixon, Doctor Xiaojing Zhou, Ms Megan Chircop, Doctor Warwick Belcher
Scheme Linkage Infrastructure Equipment & Facilities (LIEF)
Role Investigator
Funding Start 2010
Funding Finish 2010
GNo G0190402
Type Of Funding Scheme excluded from IGS
Category EXCL
UON Y

Laser microdissection microscopy system for cell and development biology$350,000

Funding body: ARC (Australian Research Council)

Funding body ARC (Australian Research Council)
Project Team Professor Eileen McLaughlin, Conjoint Professor Keith Jones, Laureate Professor John Aitken, Professor Brett Nixon, Doctor Shaun Roman, Professor Alan Brichta, Doctor Rick Thorne, Associate Professor Doug Smith, Associate Professor David McCurdy, Emeritus Professor Ray Rose, Professor Christopher Grof, Emeritus Professor Leonie Ashman, Professor Gordon Burns, Associate Professor Brett Graham, Associate Professor Paul Tooney, Laureate Professor Roger Smith, Laureate Professor Paul Foster, Professor Trevor Day, Conjoint Professor Robert Callister
Scheme Linkage Infrastructure Equipment & Facilities (LIEF)
Role Investigator
Funding Start 2010
Funding Finish 2010
GNo G0190369
Type Of Funding Scheme excluded from IGS
Category EXCL
UON Y

An Integrated LC-MS-NMR facility for Applications in Proteomics and Organic Chemistry$280,000

Funding body: University of Newcastle

Funding body University of Newcastle
Project Team Professor Adam McCluskey, Laureate Professor John Aitken, Professor Paul Dastoor, Professor Phillip Robinson, Professor Eileen McLaughlin, Emeritus Professor Geoffrey Lawrance, Emeritus Professor Marcel Maeder, Professor Hugh Dunstan, Doctor Shaun Roman, Conjoint Professor Rob Atkin, Associate Professor Clovia Holdsworth, Associate Professor Mark Baker, Associate Professor Nikki Verrills, Professor Gottfried Otting, Professor Brett Nixon, Doctor Xiaojing Zhou, Ms Megan Chircop, Doctor Warwick Belcher
Scheme Equipment Grant
Role Investigator
Funding Start 2010
Funding Finish 2010
GNo G1000873
Type Of Funding Internal
Category INTE
UON Y

Monotremes provide a key to understanding the molecular biology of mammalian gamete evolution$270,000

Funding body: ARC (Australian Research Council)

Funding body ARC (Australian Research Council)
Project Team Professor Brett Nixon, Dr Heath Ecroyd, Conjoint Associate Professor Russell Jones
Scheme Discovery Projects
Role Lead
Funding Start 2010
Funding Finish 2012
GNo G0190056
Type Of Funding Aust Competitive - Commonwealth
Category 1CS
UON Y

Laser microdissection microscopy system for cell and development biology$215,000

Funding body: University of Newcastle

Funding body University of Newcastle
Project Team Professor Eileen McLaughlin, Conjoint Professor Keith Jones, Laureate Professor John Aitken, Professor Brett Nixon, Doctor Shaun Roman, Professor Alan Brichta, Doctor Rick Thorne, Associate Professor Doug Smith, Associate Professor David McCurdy, Emeritus Professor Ray Rose, Professor Christopher Grof, Emeritus Professor Leonie Ashman, Professor Gordon Burns, Associate Professor Brett Graham, Associate Professor Paul Tooney, Laureate Professor Roger Smith, Laureate Professor Paul Foster, Professor Trevor Day, Conjoint Professor Robert Callister
Scheme Equipment Grant
Role Investigator
Funding Start 2010
Funding Finish 2010
GNo G1000874
Type Of Funding Internal
Category INTE
UON Y

Laser microdissection microscopy system for cell and development biology (HMRI contribution towards 2010 ARC LIEF grant)$50,000

Funding body: Hunter Medical Research Institute

Funding body Hunter Medical Research Institute
Project Team Professor Eileen McLaughlin, Conjoint Professor Keith Jones, Laureate Professor John Aitken, Professor Brett Nixon, Doctor Shaun Roman, Professor Alan Brichta, Doctor Rick Thorne, Associate Professor Doug Smith, Associate Professor David McCurdy, Emeritus Professor Ray Rose, Professor Christopher Grof, Emeritus Professor Leonie Ashman, Professor Gordon Burns, Associate Professor Brett Graham, Associate Professor Paul Tooney, Laureate Professor Roger Smith, Laureate Professor Paul Foster, Professor Trevor Day, Conjoint Professor Robert Callister
Scheme Equipment Grant
Role Investigator
Funding Start 2010
Funding Finish 2010
GNo G1000144
Type Of Funding Other Public Sector - State
Category 2OPS
UON Y

Waters, Ultra high performance liquid chromatography system (nano-Aquity) $30,000

Funding body: NHMRC (National Health & Medical Research Council)

Funding body NHMRC (National Health & Medical Research Council)
Project Team Associate Professor Mark Baker, Laureate Professor John Aitken, Professor Adam McCluskey, Professor Brett Nixon, Emeritus Professor John Rostas, Professor Eileen McLaughlin
Scheme Equipment Grant
Role Investigator
Funding Start 2010
Funding Finish 2010
GNo G1000051
Type Of Funding Other Public Sector - Commonwealth
Category 2OPC
UON Y

20095 grants / $1,319,000

Characterization of the molecular basis of human sperm-oocyte interaction$474,000

Funding body: NHMRC (National Health & Medical Research Council)

Funding body NHMRC (National Health & Medical Research Council)
Project Team Professor Brett Nixon, Professor Eileen McLaughlin, Dr M O'Bryan
Scheme Project Grant
Role Lead
Funding Start 2009
Funding Finish 2011
GNo G0188867
Type Of Funding Aust Competitive - Commonwealth
Category 1CS
UON Y

Confocal Laser Scanning Microscopy for Live Cell Imaging$275,000

Funding body: ARC (Australian Research Council)

Funding body ARC (Australian Research Council)
Project Team Conjoint Professor Keith Jones, Professor Eileen McLaughlin, Laureate Professor John Aitken, Emeritus Professor Ray Rose, Emeritus Professor John Patrick, Conjoint Professor Christina Offler, Associate Professor David McCurdy, Emeritus Professor Leonie Ashman, Professor Gordon Burns, Professor Dirk Van Helden, Associate Professor Nikki Verrills, Professor Brett Nixon, Doctor Shaun Roman, Professor Yong-Ling Ruan, Doctor Rick Thorne, Professor Mike Calford
Scheme Linkage Infrastructure Equipment & Facilities (LIEF)
Role Investigator
Funding Start 2009
Funding Finish 2009
GNo G0189038
Type Of Funding Scheme excluded from IGS
Category EXCL
UON Y

LAS-4000 Chemiluminescence imaging system$25,000

Funding body: NHMRC (National Health & Medical Research Council)

Funding body NHMRC (National Health & Medical Research Council)
Project Team Professor Eileen McLaughlin, Laureate Professor John Aitken, Professor Peter Lewis, Professor Brett Nixon, Doctor Shaun Roman
Scheme Equipment Grant
Role Investigator
Funding Start 2009
Funding Finish 2009
GNo G0189852
Type Of Funding Other Public Sector - Commonwealth
Category 2OPC
UON Y

20084 grants / $405,060

Xenobiotics - ocidative stress in the mammalian ovary$362,625

Funding body: NHMRC (National Health & Medical Research Council)

Funding body NHMRC (National Health & Medical Research Council)
Project Team Professor Eileen McLaughlin, Professor Brett Nixon, Doctor Shaun Roman
Scheme Project Grant
Role Investigator
Funding Start 2008
Funding Finish 2010
GNo G0187620
Type Of Funding Aust Competitive - Commonwealth
Category 1CS
UON Y

Understanding the aetiology of human male infertility: Elucidation of the mechanisms underpinning sperm-egg interaction$21,650

Funding body: Hunter Medical Research Institute

Funding body Hunter Medical Research Institute
Project Team Professor Brett Nixon, Professor Eileen McLaughlin
Scheme Project Grant
Role Lead
Funding Start 2008
Funding Finish 2008
GNo G0188465
Type Of Funding Contract - Aust Non Government
Category 3AFC
UON Y

Characterization of the molecular basis of mouse sperm-oocyte interaction$18,285

Funding body: University of Newcastle

Funding body University of Newcastle
Project Team Professor Brett Nixon, Professor Eileen McLaughlin
Scheme Near Miss Grant
Role Lead
Funding Start 2008
Funding Finish 2008
GNo G0188405
Type Of Funding Internal
Category INTE
UON Y

41st Annual Meeting of the Society for the Study of Reproduction $2,500

Funding body: University of Newcastle

Funding body University of Newcastle
Project Team Professor Brett Nixon
Scheme Travel Grant
Role Lead
Funding Start 2008
Funding Finish 2008
GNo G0188643
Type Of Funding Internal
Category INTE
UON Y

20074 grants / $51,471

Chemokines and spermatogenesis: roles of SDF-1 and CXCR-4 in the establishment of spermatogenesis$20,000

Funding body: University of Newcastle

Funding body University of Newcastle
Project Team Professor Eileen McLaughlin, Doctor Shaun Roman, Professor Brett Nixon
Scheme Near Miss Grant
Role Investigator
Funding Start 2007
Funding Finish 2007
GNo G0187197
Type Of Funding Internal
Category INTE
UON Y

Determination of the molecular processes involved in post-testicular development of sperm in the platypus and their relationship with sperm competition$16,471

Funding body: University of Newcastle

Funding body University of Newcastle
Project Team Professor Brett Nixon, Conjoint Associate Professor Russell Jones
Scheme Near Miss Grant
Role Lead
Funding Start 2007
Funding Finish 2007
GNo G0187188
Type Of Funding Internal
Category INTE
UON Y

Validation of novel model to explain the functional activation of mouse spermatozoa$7,998

Funding body: University of Newcastle

Funding body University of Newcastle
Project Team Professor Brett Nixon
Scheme Pilot Grant
Role Lead
Funding Start 2007
Funding Finish 2007
GNo G0187878
Type Of Funding Internal
Category INTE
UON Y

Validation of novel model to explain the functional activation of mouse spermatozoa$7,002

Funding body: University of Newcastle - Faculty of Science & IT

Funding body University of Newcastle - Faculty of Science & IT
Project Team Professor Brett Nixon
Scheme Pilot Grant
Role Lead
Funding Start 2007
Funding Finish 2007
GNo G0188360
Type Of Funding Internal
Category INTE
UON Y

20061 grants / $544,282

PRC - Priority Research Centre for Reproductive Science$544,282

Funding body: University of Newcastle

Funding body University of Newcastle
Project Team Laureate Professor John Aitken, Laureate Professor Roger Smith, Professor Eileen McLaughlin, Professor Brett Nixon, Doctor Shaun Roman, Conjoint Associate Professor Vicki Clifton, Conjoint Professor Warwick Giles, Professor Jon Hirst, Conjoint Associate Professor Rick Nicholson, Professor Ian Symonds
Scheme Priority Research Centre
Role Investigator
Funding Start 2006
Funding Finish 2013
GNo G0186945
Type Of Funding Internal
Category INTE
UON Y

20051 grants / $9,000

The initiation of fertilisation: Characterisation of the sperm receptor complex for the oocyte$9,000

Funding body: University of Newcastle

Funding body University of Newcastle
Project Team Professor Brett Nixon, Laureate Professor John Aitken
Scheme Project Grant
Role Lead
Funding Start 2005
Funding Finish 2005
GNo G0184742
Type Of Funding Internal
Category INTE
UON Y

20043 grants / $29,000

Characterisation of a novel recepto-mediated signal transduction pathway in human spermatozoa.$14,000

Funding body: University of Newcastle

Funding body University of Newcastle
Project Team Professor Brett Nixon, Emeritus Professor Leonie Ashman
Scheme Project Grant
Role Lead
Funding Start 2004
Funding Finish 2004
GNo G0183444
Type Of Funding Internal
Category INTE
UON Y

Molecular basis of sperm-egg interaction: Identification of the receptor complex for ZP3$8,000

Funding body: University of Newcastle

Funding body University of Newcastle
Project Team Professor Brett Nixon, Doctor Benjamin Curry
Scheme Project Grant
Role Lead
Funding Start 2004
Funding Finish 2004
GNo G0183415
Type Of Funding Internal
Category INTE
UON Y

The role of the father in recurrent early pregnancy loss$7,000

Funding body: Hunter Medical Research Institute

Funding body Hunter Medical Research Institute
Project Team Professor Brett Nixon
Scheme Adamstown Lions Club
Role Lead
Funding Start 2004
Funding Finish 2004
GNo G0183756
Type Of Funding Contract - Aust Non Government
Category 3AFC
UON Y

20021 grants / $12,000

The role of protein tyrosine phosphorylation in mouse sperm-oocyte interaction$12,000

Funding body: University of Newcastle

Funding body University of Newcastle
Project Team Professor Brett Nixon
Scheme Early Career Researcher Grant
Role Lead
Funding Start 2002
Funding Finish 2002
GNo G0182255
Type Of Funding Internal
Category INTE
UON Y

20011 grants / $15,000

The role of protein tyrosine phosphorylation in mouse sperm-oocyte interaction$15,000

Funding body: University of Newcastle

Funding body University of Newcastle
Project Team Professor Brett Nixon
Scheme Early Career Researcher Grant
Role Lead
Funding Start 2001
Funding Finish 2001
GNo G0180831
Type Of Funding Internal
Category INTE
UON Y
Edit

Research Supervision

Number of supervisions

Completed24
Current11

Current Supervision

Commenced Level of Study Research Title Program Supervisor Type
2020 PhD Exploring ALOX15 as a Molecular Conduit between Infertility and Systemic Ill Health in Men PhD (Biological Sciences), Faculty of Science, The University of Newcastle Co-Supervisor
2020 PhD Improving Development of Cultivated Spermatogonial Stem Cells Using Novel Growth Factor Receptors PhD (Biological Sciences), Faculty of Science, The University of Newcastle Co-Supervisor
2020 PhD Novel Roles for Seminal Fluid Extracellular Vesicles PhD (Biological Sciences), Faculty of Science, The University of Newcastle Co-Supervisor
2019 PhD Exploring Protein Aggregation in the Male Germ Line in Response to Oxidative Stress PhD (Biological Sciences), Faculty of Science, The University of Newcastle Co-Supervisor
2019 PhD The Development of Novel Materials for the Selection of High Quality Spermatozoa and the Assessment of Cellular Oxidative Status Through the Analysis of Lipid Peroxide Metabolites PhD (Biological Sciences), Faculty of Science, The University of Newcastle Co-Supervisor
2019 PhD The Role of Autophagy in Oocyte Development and Ageing PhD (Medical Biochemistry), Faculty of Health and Medicine, The University of Newcastle Co-Supervisor
2019 Masters Developing a Novel Diagnostic Test for Early Pregnancy of the Mare and Find out the Embryo Endometrial Interaction M Philosophy (Biological Sc), Faculty of Science, The University of Newcastle Principal Supervisor
2019 Masters The Application of Chemical Cross-linking Mass Spectrometry towards Understanding the Protein Interactome in Human Spermatozoa M Philosophy (Biological Sc), Faculty of Science, The University of Newcastle Co-Supervisor
2017 PhD Development of a Novel Injectable Chemical Agent for the Sterilisation of Cats and Dogs PhD (Biological Sciences), Faculty of Science, The University of Newcastle Co-Supervisor
2017 PhD Epididymosomes and Environmental Epigenetic Inheritance PhD (Biological Sciences), Faculty of Science, The University of Newcastle Principal Supervisor
2017 PhD Regulation of Sperm Function and Oxidative Stress by Lipoxygenase Enzymes PhD (Biological Sciences), Faculty of Science, The University of Newcastle Co-Supervisor

Past Supervision

Year Level of Study Research Title Program Supervisor Type
2020 PhD The Impact of the Environmental Hazard, Acrylamide, on the Male Reproductive Tract and Transgenerational Phenotype PhD (Biological Sciences), Faculty of Science, The University of Newcastle Co-Supervisor
2019 PhD Molecular Mechanisms Contributing to the Age-Related Decline in Oocyte Quality PhD (Biological Sciences), Faculty of Science, The University of Newcastle Principal Supervisor
2019 PhD Quality Control Mechanisms Responsible for the Maintenance of Genomic Integrity in the Female Germline PhD (Biological Sciences), Faculty of Science, The University of Newcastle Principal Supervisor
2019 PhD Functional Characterisation of Dynamin in Spermatozoa Epididymal Maturation and Acrosomal Exocytosis PhD (Biological Sciences), Faculty of Science, The University of Newcastle Principal Supervisor
2018 PhD Investigating the Effects of Mobile Phone Radiation and Heating on Spermatogenesis and Sperm Function PhD (Biological Sciences), Faculty of Science, The University of Newcastle Co-Supervisor
2018 PhD Post-Meiotic DNA Damage in Spermatozoa and Consequences to Offspring PhD (Biological Sciences), Faculty of Science, The University of Newcastle Co-Supervisor
2018 Masters Investigating the Profile of miRNAs in the Mammalian Male Reproductive Tract M Philosophy (Biological Sc), Faculty of Science, The University of Newcastle Principal Supervisor
2017 PhD The Consequences of Paternal Acrylamide Exposure and Potential for Amelioration PhD (Biological Sciences), Faculty of Science, The University of Newcastle Co-Supervisor
2017 PhD Investigation of Equine Fertility Mechanisms through Proteomic Analysis of Stallion Spermatozoa, Early Equine Embryos and Pregnant Mare Uterine Fluid PhD (Biological Sciences), Faculty of Science, The University of Newcastle Co-Supervisor
2017 PhD Towards Non-Surgical Sterilisation of Feral Horses: Biomarkers of Folliculogenesis and Covalent Modification of Proteins Using Electrophilic Aldehydes PhD (Biological Sciences), Faculty of Science, The University of Newcastle Co-Supervisor
2016 Masters Insulin Production in the Mouse Uterus M Philosophy (Biological Sc), Faculty of Science, The University of Newcastle Co-Supervisor
2015 PhD Functional Characterisation of Dynamin in Mouse Spermatozoa PhD (Biological Sciences), Faculty of Science, The University of Newcastle Principal Supervisor
2015 PhD The Function of Molecular Chaperones in Human Sperm-Egg Recognition PhD (Biological Sciences), Faculty of Science, The University of Newcastle Principal Supervisor
2015 PhD The Role of Reactive Oxygen Species and Oxidative Stress in Post-Ovulatory Ageing and Apoptosis of the Mammalian Oocyte PhD (Biological Sciences), Faculty of Science, The University of Newcastle Co-Supervisor
2014 PhD The Consequences of Acrylamide Exposure of the Male Germ Line PhD (Biological Sciences), Faculty of Science, The University of Newcastle Co-Supervisor
2013 PhD Regulation of the Transition from Gonocytes to Spermatogonia PhD (Biological Sciences), Faculty of Science, The University of Newcastle Co-Supervisor
2013 PhD New Insights into the Molecular Basis of Human Sperm-Egg Interaction PhD (Biological Sciences), Faculty of Science, The University of Newcastle Principal Supervisor
2012 PhD The Molecular Basis of Sperm - Oocyte Interactions PhD (Biological Sciences), Faculty of Science, The University of Newcastle Principal Supervisor
2012 PhD Xenobiotics; Effects on Female Fertility PhD (Biological Sciences), Faculty of Science, The University of Newcastle Co-Supervisor
2010 PhD Significance of Mitochondrial Reactive Oxygen Species Production in Male Infertility PhD (Biological Sciences), Faculty of Science, The University of Newcastle Co-Supervisor
2009 PhD The Molecular Basis of Human Sperm-Zona Pellucida Interaction PhD (Biological Sciences), Faculty of Science, The University of Newcastle Co-Supervisor
2007 Masters Identification of NADPH Oxidase in Mouse Spermatozoa M Philosophy (Biological Sc), Faculty of Science, The University of Newcastle Co-Supervisor
2007 PhD The Development of a Novel Electrophoretic System for the Isolation of Human Spermatozoa PhD (Biological Sciences), Faculty of Science, The University of Newcastle Co-Supervisor
2005 PhD The Molecular Basis of Sperm-Oocyte Interaction in the Mouse PhD (Biological Sciences), Faculty of Science, The University of Newcastle Co-Supervisor
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Research Collaborations

The map is a representation of a researchers co-authorship with collaborators across the globe. The map displays the number of publications against a country, where there is at least one co-author based in that country. Data is sourced from the University of Newcastle research publication management system (NURO) and may not fully represent the authors complete body of work.

Country Count of Publications
Australia 142
New Zealand 17
United States 12
France 11
Netherlands 6
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News

NHMRC awards $9.3 million to 13 University of Newcastle projects

December 18, 2019

The University of Newcastle has received more than $9.3 million in funding to support projects aiming to solve some of the world’s most critical health problems and improve the lives of millions of Australians.

Funding to develop targeted health solutions

August 13, 2018

Researchers from the University of Newcastle have received more than $1.4 million in funding from the National Health and Medical Research Council (NHMRC) to support the Australian Government’s new male and female health strategies.

New hope to stop the female biological clock

July 25, 2017

UON researchers have identifyied an antioxidant which has demonstrated potential to halt the aging process in female eggs.

The male reproductive mechanism causing genetic change

August 31, 2016

A new research finding from the University of Newcastle and HMRI confirms that a healthy lifestyle can influence sperm performance.

Saltwater crocodile sperm may hold secret to male fertility

May 4, 2016

Saltwater crocodiles may solve male fertility issues.

ARC Discovery Project funding success

November 19, 2014

Laureate Professor John Aitken and Associate Professor Brett Nixon have been awarded more than $403,000 in ARC Discovery Project funding commencing in 2015 for

ARC Future Fellows

July 23, 2014

UON secures record funding under 2014 scheme

Professor Brett Nixon

Position

NHMRC Reseach Fellow
Reproductive Science Group, Priority Research Centre for Reproductive Science
School of Environmental and Life Sciences
Faculty of Science

Focus area

Biological Sciences

Contact Details

Email brett.nixon@newcastle.edu.au
Phone (02) 4921 6977
Fax (02) 4921 6923

Office

Room LS4-40
Building Life Sciences.
Location Callaghan
University Drive
Callaghan, NSW 2308
Australia
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