Dr Diane Rebourcet

Dr Diane Rebourcet

Postdoctoral Research Associate

Office PVC - Science

Career Summary

Biography

After attending fascinating lectures in Life Sciences, while undertaking my undergraduate studies at Paris-Denis-Diderot University, France, I decided to specialise in Reproduction and Development and completed a Masters degree in that same institution. I then began a PhD focussing on the impact of environmental endocrine disruptors on the reproductive system at Lyon-Claude-Bernard University, France. In 2011, thanks to a fellowship from the Servier Institute (a non-profit association), I obtained funding to pursue my postdoctoral studies at the University of Edinburgh in the Centre for Reproductive Health, and in 2018, I was recruited by the University of Newcastle as a Postdoctoral Research Associate.

Research expertise

My work includes a variety of subjects that reflect my research interests from when I first graduated to present day. From an early start in biological sciences, I carried out research in the area of reproduction, mainly focusing on reproductive pathologies and starting with the female reproductive system in relation with ovarian disorders (PCOS and premature ovarian failure). During my postgraduate studies, I felt especially pertained by the impact and consequences of the environment on human health, and therefore, extended my research interests to reproductive toxicology and endocrine disruption. Undertaking my PhD and first postdoctoral position in this field, I had the opportunity to address the impact of endocrine disruption on the male reproductive system development and function. My current research focuses on understanding the mechanisms and regulations affecting the reproductive system, amongst which the androgen biosynthesis (mainly undertaken by the Leydig cells) and in developing new approaches for potential therapies to benefit male health.

Current aspects of research:

  • The recent identification of new roles for Sertoli cells has fundamentally changed our understanding of testis biology regarding its development and function. My research interest is to pursue the characterisation of Sertoli cell roles and to utilise their properties as biological therapeutical tools.
  • Demonstrating the complexity of Sertoli cell regulation of Leydig cell development and function, Sertoli cells appear as a novel target for endocrine disruption and/or potential therapy to improve androgen profile. One focus on my ongoing research is to identify Sertoli cell factors that can regulate Leydig cells function,
  • With the recent identification of differential androgens pathways (classical and backdoor), another aspect of my ongoing research focuses on deciphering the importance of both classical and backdoor androgen pathways on testis development and function.
  • With a better understanding of androgen regulation, finding targeted and suitable therapies is another topic of my current research.

Common approaches: transgenic mouse models, “gene therapy”, biotechnologies, in silico modelling as well as usual bio-molecular techniques.

Teaching expertise

I have lectured for the University of Edinburgh in the Reproductive Systems (BIME10016) Undergraduate 4th year Course since 2014. I had the opportunity during my position in Edinburgh to be involved in the day-to-day supervision of PhD students and to supervise laboratory projects for both Bachelor of Science (Reproductive Biology) and Master of Science (Reproductive Science) students, both of which graduated with distinction.

Collaborations

I am working in close collaboration with world leading researchers: Peter O’Shaughnessy (University of Glasgow), Rod Mitchell (University of Edinburgh), Serge Nef (University of Geneva), Peter Stanton and Liza O’Donnell (Hudson Institute of Medical Research).


If you are a prospective Honours/PhD student and interested in joining the laboratory, please contact me via email: diane.rebourcet@newcastle.edu.au



Qualifications

  • PhD (Reproductive Biology), Claude Bernard University Lyon 1, France
  • Master in Cellular Biology Physiology & Pathology, Claude Bernard University Lyon 1, France

Keywords

  • Biotechnology
  • Endocrine disruptors
  • Endocrinology
  • Fertility
  • Infertility
  • Reproductive Biology
  • Reproductive Sciences

Languages

  • French (Mother)
  • English (Fluent)

Fields of Research

Code Description Percentage
060803 Animal Developmental and Reproductive Biology 45
060699 Physiology not elsewhere classified 15
110306 Endocrinology 40

Professional Experience

UON Appointment

Title Organisation / Department
Postdoctoral Research Associate University of Newcastle
Office PVC - Science
Australia

Academic appointment

Dates Title Organisation / Department
17/06/2015 - 31/03/2018 Postdoctoral Research fellow University of Edinburgh
Medical Research Council (MRC) Centre for Reproductive Health
United Kingdom
18/06/2012 - 19/06/2015 Post-doctorate researcher University of Edinburgh
Medical Research Council (MRC) Centre for Reproductive Health
United Kingdom
15/06/2011 - 17/06/2012 Post-doctorate researcher University of Edinburgh
Medical Research Council (MRC) Centre for Reproductive Health
United Kingdom

Invitations

Keynote Speaker

Year Title / Rationale
2018 Effect of Sertoli cell ablation on testicular development and function.
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Publications

For publications that are currently unpublished or in-press, details are shown in italics.


Chapter (1 outputs)

Year Citation Altmetrics Link
2018 Rebourcet D, Darbey A, Curley M, O Shaughnessy P, Smith LB, 'Testicular cell selective ablation using diphtheria toxin receptor transgenic mice', Sertoli Cells: Methods and Protocols, Humana Press, New York, NY 203-228 (2018) [B1]
DOI 10.1007/978-1-4939-7698-0_15
Co-authors Lee Smith

Journal article (15 outputs)

Year Citation Altmetrics Link
2018 Curley M, Milne L, Smith S, Atanassova N, Rebourcet D, Darbey A, et al., 'Leukemia Inhibitory Factor-Receptor is Dispensable for Prenatal Testis Development but is Required in Sertoli cells for Normal Spermatogenesis in Mice', SCIENTIFIC REPORTS, 8 (2018) [C1]
DOI 10.1038/s41598-018-30011-w
Co-authors Lee Smith
2017 van den Driesche S, Kilcoyne KR, Wagner I, Rebourcet D, Boyle A, Mitchell R, et al., 'Experimentally induced testicular dysgenesis syndrome originates in the masculinization programming window', JCI Insight, 2 (2017)
DOI 10.1172/jci.insight.91204
2017 Soffientini U, Rebourcet D, Abel MH, Lee S, Hamilton G, Fowler PA, et al., 'Identification of Sertoli cell-specific transcripts in the mouse testis and the role of FSH and androgen in the control of Sertoli cell activity', BMC GENOMICS, 18 (2017) [C1]
DOI 10.1186/s12864-017-4357-3
Co-authors Lee Smith
2017 Rebourcet D, Darbey A, Monteiro A, Soffientini U, Tsai YT, Handel I, et al., 'Sertoli Cell Number Defines and Predicts Germ and Leydig Cell Population Sizes in the Adult Mouse Testis', ENDOCRINOLOGY, 158 2955-2969 (2017) [C1]
DOI 10.1210/en.2017-00196
Citations Scopus - 6Web of Science - 5
Co-authors Lee Smith
2017 Patel SH, O'Hara L, Atanassova N, Smith SE, Curley MK, Rebourcet D, et al., 'Low-dose tamoxifen treatment in juvenile males has long-term adverse effects on the reproductive system: implications for inducible transgenics.', Scientific Reports, 7 (2017) [C1]
DOI 10.1038/s41598-017-09016-4
Citations Scopus - 3Web of Science - 2
Co-authors Annelouise Gannon, Lee Smith
2016 Rebourcet LB, Wu J, Cruickshanks L, Smith SE, Milne L, Fernando A, et al., 'Sertoli cells modulate testicular vascular network development, structure, and function to influence circulating testosterone concentrations in adult male mice', Endocrinology, 157 2479-2488 (2016) [C1]
DOI 10.1210/en.2016-1156
Citations Scopus - 7Web of Science - 6
Co-authors Lee Smith
2015 Guillermet-Guibert J, Smith LB, Halet G, Whitehead MA, Pearce W, Rebourcet D, et al., 'Novel Role for p110 beta PI 3-Kinase in Male Fertility through Regulation of Androgen Receptor Activity in Sertoli Cells', PLOS GENETICS, 11 (2015) [C1]
DOI 10.1371/journal.pgen.1005304
Citations Scopus - 8Web of Science - 8
Co-authors Lee Smith
2015 Smith LB, O'Shaughnessy PJ, Rebourcet D, 'Cell-specific ablation in the testis: what have we learned?', ANDROLOGY, 3 1035-1049 (2015) [C1]
DOI 10.1111/andr.12107
Citations Scopus - 11Web of Science - 11
Co-authors Lee Smith
2014 Rebourcet D, O'Shaughnessy PJ, Pitetti J-L, Monteiro A, O'Hara L, Milne L, et al., 'Sertoli cells control peritubular myoid cell fate and support adult Leydig cell development in the prepubertal testis', DEVELOPMENT, 141 2139-2149 (2014) [C1]
DOI 10.1242/dev.107029
Citations Scopus - 35Web of Science - 31
Co-authors Lee Smith
2014 Rebourcet D, O'Shaughnessy PJ, Monteiro A, Milne L, Cruickshanks L, Jeffrey N, et al., 'Sertoli Cells Maintain Leydig Cell Number and Peritubular Myoid Cell Activity in the Adult Mouse Testis', PLOS ONE, 9 (2014) [C1]
DOI 10.1371/journal.pone.0105687
Citations Scopus - 33Web of Science - 28
Co-authors Lee Smith
2012 Magre S, Rebourcet D, Ishaq M, Wargnier R, Debard C, Meugnier E, et al., 'Gender Differences in Transcriptional Signature of Developing Rat Testes and Ovaries following Embryonic Exposure to 2,3,7,8-TCDD', PLoS ONE, 7 e40306-e40306 (2012)
DOI 10.1371/journal.pone.0040306
2011 Naville D, Rebourcet D, Chauvin M-A, Vega N, Jalabert A, Vigier M, et al., 'Direct and indirect impact of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on adult mouse Leydig cells: An in vitro study', Toxicology Letters, 207 251-257 (2011)
DOI 10.1016/j.toxlet.2011.09.019
2010 Bachelot A, Bouilly J, Liu Y, Rebourcet D, Leux C, Kuttenn F, et al., 'Sequence variation analysis of the prolactin receptor C-terminal region in women with premature ovarian failure', Fertility and Sterility, 94 2772-2775 (2010)
DOI 10.1016/j.fertnstert.2010.06.040
2010 Rebourcet D, Odet F, Vérot A, Combe E, Meugnier E, Pesenti S, et al., 'The effects of an in utero exposure to 2,3,7,8-tetrachloro-dibenzo- p -dioxin on male reproductive function: identification of Ccl5 as a potential marker', International Journal of Andrology, 33 413-424 (2010)
DOI 10.1111/j.1365-2605.2009.01020.x
2008 Halperin J, Devi SY, Elizur S, Stocco C, Shehu A, Rebourcet D, et al., 'Prolactin Signaling through the Short Form of Its Receptor Represses Forkhead Transcription Factor FOXO3 and Its Target Gene Galt Causing a Severe Ovarian Defect', Molecular Endocrinology, 22 513-522 (2008)
DOI 10.1210/me.2007-0399
Show 12 more journal articles
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Grants and Funding

Summary

Number of grants 5
Total funding $73,294

Click on a grant title below to expand the full details for that specific grant.


20161 grants / $1,000

Society For Endocrinology travel grant $1,000

Funding body: Society For Endocrinology

Funding body Society For Endocrinology
Project Team

Dr Diane Rebourcet

Scheme SFE-Travel grant
Role Lead
Funding Start 2016
Funding Finish 2016
GNo
Type Of Funding External
Category EXTE
UON N

20151 grants / $18,000

Determining the Testicular Interstitial Fluid Proteome: Identification of Sertoli cell-derived paracrine factors regulating lifelong Leydig cell function.$18,000

Funding body: Society For Endocrinology

Funding body Society For Endocrinology
Project Team

Dr Diane Rebourcet (CIA), Prof Lee Smith, Dr Peter Stanton, Dr Liza O'Donnell

Scheme SFE-Early Career Grant
Role Lead
Funding Start 2015
Funding Finish 2015
GNo
Type Of Funding External
Category EXTE
UON N

20121 grants / $3,614

MSc Reproductive Science studentship, University of Edinburgh.$3,614

Funding body: MSc Reproductive Science studentship

Funding body MSc Reproductive Science studentship
Project Team

Dr Diane Rebourcet (PI), Annalucia Darbey (student)

Scheme MSc Reproductive Science studentship, University of Edinburgh.
Role Lead
Funding Start 2012
Funding Finish 2013
GNo
Type Of Funding Internal
Category INTE
UON N

20111 grants / $31,680

Timing and regulation of Sertoli cell proliferation in perinatal life and its vulnerability to disruption by lifestyle/environmental chemical exposures.$31,680

Funding body: Research funding from Servier Association

Funding body Research funding from Servier Association
Project Team

Dr Diane Rebourcet (CIA), Pr Richard Sharpe

Scheme Research funding
Role Lead
Funding Start 2011
Funding Finish 2012
GNo
Type Of Funding External
Category EXTE
UON N

20101 grants / $19,000

Effects of an in Utero Exposure to 2,3-7,8 TCDD on the rat reproductive system and role of the aryl hydrocarbon receptor in mouse testicular physiology.$19,000

Funding body: Schering-Plough research funds

Funding body Schering-Plough research funds
Project Team

Diane Rebourcet (CIA), Dr Brigitte Battistoni-Le Magueresse

Scheme Research funds
Role Investigator
Funding Start 2010
Funding Finish 2010
GNo
Type Of Funding External
Category EXTE
UON N
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Research Opportunities

Importance of the classical and backdoor androgen pathways in testis development and function.

PhD project

PHD

Faculty of Science

14/01/2019 - 15/06/2021

Contact

Doctor Diane Rebourcet
University of Newcastle
Office PVC - Science
diane.rebourcet@newcastle.edu.au

Development of Sertoli cells as biological tools.

research project

Honours

Faculty of Science

14/01/2019 - 20/11/2019

Contact

Doctor Diane Rebourcet
University of Newcastle
Office PVC - Science
diane.rebourcet@newcastle.edu.au

Regulation of testis development and function.

research project

Honours

Faculty of Science

14/01/2019 - 20/11/2019

Contact

Doctor Diane Rebourcet
University of Newcastle
Office PVC - Science
diane.rebourcet@newcastle.edu.au

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Dr Diane Rebourcet

Position

Postdoctoral Research Associate
Lee Smith Group
Office PVC - Science
Faculty of Science

Contact Details

Email diane.rebourcet@newcastle.edu.au
Phone (02) 49854598
Fax (02) 49216308

Office

Room Life Sciences Building - LS2.02
Building Life Sciences Building
Location Callaghan
University Drive
Callaghan, NSW 2308
Australia
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