2024 |
Santhanes D, Zhang H, Wilkins A, Aitken RJ, Gannon A-L, Liang M, 'Precise control of microfluidic flow conditions is critical for harnessing the in vitro transfection capability of pDNA-loaded lipid-Eudragit nanoparticles.', Drug Deliv Transl Res, (2024) [C1]
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2024 |
Hungerford AJ, Bakos HW, Aitken RJ, 'Addition of Vitamin C Mitigates the Loss of Antioxidant Capacity, Vitality and DNA Integrity in Cryopreserved Human Semen Samples.', Antioxidants (Basel), 13 (2024) [C1]
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Nova |
2023 |
Aitken RJ, 'Are the MiOXSYS and OxiSpermII assays suitable for detecting oxidative stress in human semen samples?', ANDROLOGY, 11 1579-1580 (2023)
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2023 |
Hungerford A, Bakos HW, Aitken RJ, 'Sperm cryopreservation: current status and future developments', REPRODUCTION FERTILITY AND DEVELOPMENT, [C1]
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Nova |
2023 |
Aitken RJ, Lambourne S, Medica AJ, 'Predicting the outcome of Thoroughbred stallion matings on the basis of dismount semen sample analyses.', Reproduction, 165 281-288 (2023) [C1]
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Nova |
2023 |
Aitken RJ, 'Sperm DNA integrity: a special issue exploring the causes, consequences, and treatment of DNA damage in human spermatozoa', ANDROLOGY, 11 1541-1544 (2023)
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2023 |
Shapouri F, Mahendran T, Govindarajan M, Xie P, Kocur O, Palermo GD, et al., 'A comparison between the Felix electrophoretic system of sperm isolation and conventional density gradient centrifugation: a multicentre analysis', Journal of Assisted Reproduction and Genetics, 40 83-95 (2023) [C1]
Purpose: Developing optimized techniques for the isolation of human spermatozoa possessing low levels of DNA damage is an important objective for the ART industry. The purpose of ... [more]
Purpose: Developing optimized techniques for the isolation of human spermatozoa possessing low levels of DNA damage is an important objective for the ART industry. The purpose of this study was to compare a novel electrophoretic system (Felix¿) of sperm isolation with a conventional method involving density gradient centrifugation (DGC). Methods: Five international ART Centres in Australia, India, Sweden, the USA, and China have collaborated in order to compare the quality of the sperm populations isolated by Felix¿ and DGC in terms of processing time, sperm concentration, motility, vitality, and DNA integrity as assessed by 3 methods: SCSA, Halo, and TUNEL. Results: Across all centers, 112 comparisons were performed. Although significant differences were noted between centers in terms of the quality of the semen samples subjected for analysis, overall, both methods were equally capable of isolating populations of spermatozoa exhibiting high levels of vitality and progressive motility. The absolute numbers of spermatozoa recovered were significantly (p < 0.001) lower with the Felix¿ device although sperm quality was higher with 4/5 centers reporting a significant improvement in DNA integrity relative to DGC (p < 0.01¿p < 0.001). In practical terms, the Felix¿ device featured a standardized 6¿min preparation time whereas clinical DGC protocols varied from center to center but generally took around 40¿min to complete. Conclusions: The Felix¿ device is a positive technical development capable of isolating suspensions of highly motile spermatozoa exhibiting low levels of DNA damage in a fraction of the time taken by conventional procedures such as DGC.
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Nova |
2023 |
Fraser BA, Wilkins AL, De Iuliis GN, Rebourcet D, Nixon B, Aitken RJ, 'Development of a model for studying the developmental consequences of oxidative sperm DNA damage by targeting redox-cycling naphthoquinones to the Sertoli cell population.', Free radical biology & medicine, 206 50-62 (2023) [C1]
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Nova |
2023 |
Hungerford AJ, Bakos HW, Aitken RJ, 'Analysis of sperm separation protocols for isolating cryopreserved human spermatozoa.', Reproduction & fertility, 4 e220133 (2023) [C1]
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Nova |
2023 |
Samarasinghe SVAC, Krishnan K, Aitken RJ, Naidu R, Megharaj M, 'Chronic effects of TiO2 and ZnO nanoparticles to earthworm Eisenia fetida', Environmental Chemistry and Ecotoxicology, 5 129-134 (2023) [C1]
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Nova |
2023 |
Aitken RJ, 'Male reproductive ageing: a radical road to ruin.', Hum Reprod, 38 1861-1871 (2023) [C1]
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Nova |
2023 |
Hug E, Villeneuve P, Bravard S, Chorfa A, Damon-Soubeyrand C, Somkuti SG, et al., 'Loss of Nuclear/DNA Integrity in Mouse Epididymal Spermatozoa after Short-Term Exposure to Low Doses of Dibutyl Phthalate or Bisphenol AF and Its Mitigation by Oral Antioxidant Supplementation.', Antioxidants (Basel), 12 (2023) [C1]
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Nova |
2023 |
Aitken RJ, Lewis SEM, 'DNA damage in testicular germ cells and spermatozoa. When and how is it induced? How should we measure it? What does it mean?', Andrology, 11 1545-1557 (2023) [C1]
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Nova |
2023 |
Vidane Arachchige Chamila Samarasinghe S, Krishnan K, John Aitken R, Naidu R, Megharaj M, 'Multigenerational effects of TiO2 rutile nanoparticles on earthworms.', Environ Pollut, 336 122376 (2023) [C1]
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Nova |
2023 |
Behdarvandian P, Nasr-Esfahani A, Tavalaee M, Pashaei K, Naderi N, Darmishonnejad Z, et al., 'Sperm chromatin structure assay (SCSA®) and flow cytometry-assisted TUNEL assay provide a concordant assessment of sperm DNA fragmentation as a function of age in a large cohort of approximately 10,000 patients.', Basic Clin Androl, 33 33 (2023) [C1]
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Nova |
2023 |
Germon ZP, Sillar JR, Mannan A, Duchatel RJ, Staudt D, Murray HC, et al., 'Blockade of ROS production inhibits oncogenic signaling in acute myeloid leukemia and amplifies response to precision therapies.', Sci Signal, 16 eabp9586 (2023) [C1]
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Nova |
2023 |
Medica AJ, Lambourne S, Aitken RJ, 'Predicting the Outcome of Equine Artificial Inseminations Using Chilled Semen.', Animals (Basel), 13 (2023) [C1]
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Nova |
2023 |
Lawson EF, Ghosh A, Blanch V, Grupen CG, Aitken RJ, Lim R, et al., 'Establishment and characterization of oviductal organoids from farm and companion animals .', Biol Reprod, 108 854-865 (2023) [C1]
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Nova |
2022 |
Aitken RJ, 'Role of sperm DNA damage in creating de-novo mutations in human offspring: the post-meiotic oocyte collusion hypothesis', Reproductive BioMedicine Online, 45 109-124 (2022) [C1]
Spermatogonial stem cells exhibit a low level of spontaneous mutation that is heavily impacted by paternal age via mechanisms that appear to involve the aberrant repair of DNA dam... [more]
Spermatogonial stem cells exhibit a low level of spontaneous mutation that is heavily impacted by paternal age via mechanisms that appear to involve the aberrant repair of DNA damage. This background de-novo mutation frequency can be increased 1000-fold by mutations affecting a key signal transduction pathway that confers upon its descendants a selective advantage, leading to clonal expansion and nests of mutant germ cells in the testes of ageing males. This ¿selfish selection¿ model effectively explains the origin of several dominant developmental disorders, such as achondroplasia and Apert syndrome, but cannot be generalized to account for the majority of de-novo mutations where no selective advantage is apparent. In this article, an additional germline mutation pathway is proposed that recognizes the unique susceptibility of spermatozoa to DNA damage and the importance of the oocyte in repairing these lesions prior to the S phase of the first mitotic division. Any deficiency or inaccuracy on the part of the oocyte in effecting this repair process has the potential to fix paternal DNA damage as a de-novo mutation in the embryo. Such a mechanism supports emerging data indicating that assisted conception procedures may enhance the mutational load carried by ART offspring.
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Nova |
2022 |
Aitken RJ, Bromfield E, Gibb Z, 'The impact of oxidative stress on reproduction: a focus on gametogenesis and fertilization.', Reproduction, 164 F79-F94 (2022) [C1]
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Nova |
2022 |
Moazamian A, Gharagozloo P, Aitken RJ, Drevet JR, 'OXIDATIVE STRESS AND REPRODUCTIVE FUNCTION: Sperm telomeres, oxidative stress, and infertility', REPRODUCTION, 164 F125-F133 (2022) [C1]
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Nova |
2022 |
Aitken RJ, 'The changing tide of human fertility', Human Reproduction, 37 629-638 (2022) [C1]
Over the past half-century, the world has witnessed a steep decline in fertility rates in virtually every country on Earth. This universal decline in fertility is being driven by ... [more]
Over the past half-century, the world has witnessed a steep decline in fertility rates in virtually every country on Earth. This universal decline in fertility is being driven by increasing prosperity largely through the mediation of social factors, the most powerful of which are the education of women and an accompanying shift in life's purpose away from procreation. In addition, it is clear that environmental and lifestyle factors are also having a profound impact on our reproductive competence particularly in the male where increasing prosperity is associated with a significant rise in the incidence of testicular cancer and a secular decline in semen quality and testosterone levels. On a different timescale, we should also recognize that the increased prosperity associated with the demographic transition greatly reduces the selection pressure on high fertility genes by lowering the rates of infant and childhood mortality. The retention of poor fertility genes within the human population is also being exacerbated by the increased uptake of ART. It is arguable that all of these elements are colluding to drive our species into an infertility trap. If we are to avoid the latter, it will be important to recognize the factors contributing to this phenomenon and adopt the social, political, environmental and lifestyle changes needed to bring this situation under control.
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Nova |
2022 |
Aitken RJ, Gibb Z, 'Sperm oxidative stress in the context of male infertility: current evidence, links with genetic and epigenetic factors and future clinical needs.', Minerva Endocrinol (Torino), 47 38-57 (2022) [C1]
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Nova |
2022 |
Medica AJ, Gibb Z, Sheridan A, Harrison N, Aitken RJ, 'Causative mechanisms and functional correlates of MTT reduction in stallion spermatozoa', REPRODUCTION, 163 341-350 (2022) [C1]
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Nova |
2022 |
Lawson EF, Grupen CG, Baker MA, Aitken RJ, Swegen A, Pollard CL, Gibb Z, 'Conception and early pregnancy in the mare: lipidomics the unexplored frontier', Reproduction and Fertility, 3 R1-R18 (2022) [C1]
Lipids are dynamic biological molecules that play key roles in metabolism, inflammation, cell signalling and structure. They are biologically significant in the physiology of conc... [more]
Lipids are dynamic biological molecules that play key roles in metabolism, inflammation, cell signalling and structure. They are biologically significant in the physiology of conception and reproduction. Many of the mechanisms surrounding equine conception and the early feto-maternal dialogue are yet to be understood at a biochemical level. Recently, lipidomic technologies have advanced considerably and analytical strategies have been enhanced and diversified. Consequently, in-depth lipidomic exploration now has the potential to reveal new lipid biomarkers and biochemical relationships that improve our understanding of the processes leading to efficient and successful reproduction. This review considers the role of lipids in conception and establishment of pregnancy, providing new insights into the enigmatic pathways governing early reproductive physiology of the mare.
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Nova |
2022 |
Klein EK, Swegen A, Gunn AJ, Stephen CP, Aitken RJ, Gibb Z, 'The future of assessing bull fertility: Can the 'omics fields identify usable biomarkers? .', Biol Reprod, 106 854-864 (2022) [C1]
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Nova |
2022 |
Martin JH, Nixon B, Cafe SL, Aitken RJ, Bromfield EG, Lord T, 'OXIDATIVE STRESS AND REPRODUCTIVE FUNCTION: Oxidative stress and in vitro ageing of the post-ovulatory oocyte: an update on recent advances in the field', REPRODUCTION, 164 F109-F124 (2022) [C1]
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Nova |
2022 |
Burke ND, Nixon B, Roman SD, Schjenken JE, Walters JLH, Aitken RJ, Bromfield EG, 'Male infertility and somatic health - insights into lipid damage as a mechanistic link', NATURE REVIEWS UROLOGY, 19 727-750 (2022) [C1]
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Nova |
2022 |
Minichiello M, Aitken RJ, 'Creativity as a Multidisciplinary Process', Design Principles and Practices, 16 1-17 (2022) [C1]
This paper is a result of the Plenary session centered on a discussion between Professors Aitken and Minichiello at the Design Principles Conference 2022. For many years Aitken an... [more]
This paper is a result of the Plenary session centered on a discussion between Professors Aitken and Minichiello at the Design Principles Conference 2022. For many years Aitken and Minichiello have examined ways of enabling interdisciplinary/multidisciplinary research and how this might result in real-world impact. While many academics in both fields agree that research partnerships between arts and science are desirable, examples of how this can be achieved in a university setting are not as clear. In this discussion, we consider some of the key questions and show some of the best historic practices, as well as examples of current collaborative projects. Many of these projects have been conducted through the Future Arts Science and technology laboratory (FASTlab), at the University of Newcastle NSW.
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Nova |
2022 |
Santhanes D, Wilkins A, Zhang H, John Aitken R, Liang M, 'Microfluidic formulation of lipid/polymer hybrid nanoparticles for plasmid DNA (pDNA) delivery', International Journal of Pharmaceutics, 627 122223-122223 (2022) [C1]
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Nova |
2022 |
Björndahl L, Barratt CLR, Mortimer D, Agarwal A, Aitken RJ, Alvarez JG, et al., 'Standards in semen examination: publishing reproducible and reliable data based on high-quality methodology.', Hum Reprod, 37 2497-2502 (2022) [C1]
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Nova |
2022 |
Drevet JR, Hallak J, Nasr-Esfahani M-H, Aitken RJ, 'Reactive Oxygen Species and Their Consequences on the Structure and Function of Mammalian Spermatozoa.', Antioxid Redox Signal, 37 481-500 (2022) [C1]
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Nova |
2022 |
Griffin RA, Swegen A, Baker MA, Ogle RA, Smith N, Aitken RJ, et al., 'Proteomic analysis of spermatozoa reveals caseins play a pivotal role in preventing short-term periods of subfertility in stallions .', Biol Reprod, 106 741-755 (2022) [C1]
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Nova |
2022 |
Aitken RJ, Drevet JR, Moazamian A, Gharagozloo P, 'Male Infertility and Oxidative Stress: A Focus on the Underlying Mechanisms', Antioxidants, 11 (2022) [C1]
Reactive oxygen species (ROS) play a critical role in defining the functional competence of human spermatozoa. When generated in moderate amounts, ROS promote sperm capacitation b... [more]
Reactive oxygen species (ROS) play a critical role in defining the functional competence of human spermatozoa. When generated in moderate amounts, ROS promote sperm capacitation by facilitating cholesterol efflux from the plasma membrane, enhancing cAMP generation, inducing cytoplasmic alkalinization, increasing intracellular calcium levels, and stimulating the protein phos-phorylation events that drive the attainment of a capacitated state. However, when ROS generation is excessive and/or the antioxidant defences of the reproductive system are compromised, a state of oxidative stress may be induced that disrupts the fertilizing capacity of the spermatozoa and the structural integrity of their DNA. This article focusses on the sources of ROS within this system and examines the circumstances under which the adequacy of antioxidant protection might become a limiting factor. Seminal leukocyte contamination can contribute to oxidative stress in the ejaculate while, in the germ line, the dysregulation of electron transport in the sperm mitochondria, elevated NADPH oxidase activity, or the excessive stimulation of amino acid oxidase action are all potential contributors to oxidative stress. A knowledge of the mechanisms responsible for creating such stress within the human ejaculate is essential in order to develop better antioxidant strategies that avoid the unintentional creation of its reductive counterpart.
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Nova |
2022 |
Aitken RJ, 'Oxidative stress and reproductive function', REPRODUCTION, 164 E5-E8 (2022)
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2021 |
Medica AJ, Aitken RJ, Nicolson GL, Sheridan AR, Swegen A, Iuliis GND, Gibb Z, 'Glycerophospholipids protect stallion spermatozoa from oxidative damage in vitro', Reproduction and Fertility, 2 199-209 (2021) [C1]
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Nova |
2021 |
Aitken RJ, Bakos HW, 'Should we be measuring DNA damage in human spermatozoa? New light on an old question', HUMAN REPRODUCTION, 36 1175-1185 (2021) [C1]
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Nova |
2021 |
Aitken RJ, Curry BJ, Shokri S, Pujianto DA, Gavriliouk D, Gibb Z, et al., 'Evidence that extrapancreatic insulin production is involved in the mediation of sperm survival', Molecular and Cellular Endocrinology, 526 (2021) [C1]
Evidence is presented for expression of the insulin receptor on the surface of mammalian spermatozoa as well as transcripts for the receptor substrate adaptor proteins (IRS1-4) ne... [more]
Evidence is presented for expression of the insulin receptor on the surface of mammalian spermatozoa as well as transcripts for the receptor substrate adaptor proteins (IRS1-4) needed to mediate insulin action. Exposure to this hormone resulted in insulin receptor phosphorylation (pTyr972), activation of AKT (pSer473) and the stimulation of sperm motility. Intriguingly, the male germ line is also shown to be capable of generating insulin, possessing the relevant mRNA transcript and expressing strong immunocytochemical signals for both insulin and C-peptide. Insulin could be released from the spermatozoa by sonication in a concentration-dependent manner but was not secreted in response to glucose, fructose or stimulation with progesterone. However, insulin release could be induced by factors present in human uterine lavages. Furthermore, the endometrium was also shown to possess the machinery for insulin production and action (mRNA, insulin, C-peptide, proprotein convertase and insulin receptor), releasing insulin into the uterine lumen prior to ovulation. These studies emphasize the fundamental importance of extra-pancreatic insulin in regulating the reproductive process, particularly in the support of spermatozoa on their perilous voyage to the site of fertilization.
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Nova |
2021 |
Skerrett-Byrne DA, Trigg NA, Bromfield EG, Dun MD, Bernstein IR, Anderson AL, et al., 'Proteomic dissection of the impact of environmental exposures on mouse seminal vesicle function', Molecular and Cellular Proteomics, 20 (2021) [C1]
Seminal vesicles are an integral part of the male reproductive accessory gland system. They produce a complex array of secretions containing bioactive constituents that support ga... [more]
Seminal vesicles are an integral part of the male reproductive accessory gland system. They produce a complex array of secretions containing bioactive constituents that support gamete function and promote reproductive success, with emerging evidence suggesting these secretions are influenced by our environment. Despite their significance, the biology of seminal vesicles remains poorly defined. Here, we complete the first proteomic assessment of mouse seminal vesicles and assess the impact of the reproductive toxicant acrylamide. Mice were administered acrylamide (25 mg/kg bw/day) or control daily for five consecutive days prior to collecting seminal vesicle tissue. A total of 5013 proteins were identified in the seminal vesicle proteome with bioinformatic analyses identifying cell proliferation, protein synthesis, cellular death, and survival pathways as prominent biological processes. Secreted proteins were among the most abundant, and several proteins are linked with seminal vesicle phenotypes. Analysis of the effect of acrylamide on the seminal vesicle proteome revealed 311 differentially regulated (FC ± 1.5, p = 0.05, 205 up-regulated, 106 downregulated) proteins, orthogonally validated via immunoblotting and immunohistochemistry. Pathways that initiate protein synthesis to promote cellular survival were prominent among the dysregulated pathways, and rapamycin-insensitive companion of mTOR (RICTOR, p = 6.69E-07) was a top-ranked upstream driver. Oxidative stress was implicated as contributing to protein changes, with acrylamide causing an increase in 8-OHdG in seminal vesicle epithelial cells (fivefold increase, p = 0.016) and the surrounding smooth muscle layer (twofold increase, p = 0.043). Additionally, acrylamide treatment caused a reduction in seminal vesicle secretion weight (36% reduction, p = 0.009) and total protein content (25% reduction, p = 0.017). Together these findings support the interpretation that toxicant exposure influences male accessory gland physiology and highlights the need to consider the response of all male reproductive tract tissues when interpreting the impact of environmental stressors on male reproductive function.
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Nova |
2021 |
Walters JLH, Anderson AL, da Silva SJM, Aitken RJ, De Iuliis GN, Sutherland JM, et al., 'Mechanistic insight into the regulation of lipoxygenase-driven lipid peroxidation events in human spermatozoa and their impact on male fertility', Antioxidants, 10 1-19 (2021) [C1]
A prevalent cause of sperm dysfunction in male infertility patients is the overproduction of reactive oxygen species, an attendant increase in lipid peroxidation and the productio... [more]
A prevalent cause of sperm dysfunction in male infertility patients is the overproduction of reactive oxygen species, an attendant increase in lipid peroxidation and the production of cyto-toxic reactive carbonyl species such as 4-hydroxynonenal. Our previous studies have implicated arachidonate 15-lipoxygenase (ALOX15) in the production of 4-hydroxynonenal in developing germ cells. Here, we have aimed to develop a further mechanistic understanding of the lipoxygen-ase-lipid peroxidation pathway in human spermatozoa. Through pharmacological inhibition studies, we identified a protective role for phospholipase enzymes in the liberation of peroxidised polyunsaturated fatty acids from the human sperm membrane. Our results also revealed that arachi-donic acid, linoleic acid and docosahexanoic acid are key polyunsaturated fatty acid substrates for ALOX15. Upon examination of ALOX15 in the spermatozoa of infertile patients compared to their normozoospermic counterparts, we observed significantly elevated levels of ALOX15 protein abundance in the infertile population and an increase in 4-hydroxynonenal adducts. Collectively, these data confirm the involvement of ALOX15 in the oxidative stress cascade of human spermatozoa and support the notion that increased ALOX15 abundance in sperm cells may accentuate membrane lipid peroxidation and cellular dysfunction, ultimately contributing to male infertility.
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Nova |
2021 |
Skerrett-Byrne DA, Nixon B, Bromfield EG, Breen J, Trigg NA, Stanger SJ, et al., 'Transcriptomic analysis of the seminal vesicle response to the reproductive toxicant acrylamide', BMC Genomics, 22 (2021) [C1]
Background: The seminal vesicles synthesise bioactive factors that support gamete function, modulate the female reproductive tract to promote implantation, and influence developme... [more]
Background: The seminal vesicles synthesise bioactive factors that support gamete function, modulate the female reproductive tract to promote implantation, and influence developmental programming of offspring phenotype. Despite the significance of the seminal vesicles in reproduction, their biology remains poorly defined. Here, to advance understanding of seminal vesicle biology, we analyse the mouse seminal vesicle transcriptome under normal physiological conditions and in response to acute exposure to the reproductive toxicant acrylamide. Mice were administered acrylamide (25 mg/kg bw/day) or vehicle control daily for five consecutive days prior to collecting seminal vesicle tissue 72 h following the final injection. Results: A total of 15,304 genes were identified in the seminal vesicles with those encoding secreted proteins amongst the most abundant. In addition to reproductive hormone pathways, functional annotation of the seminal vesicle transcriptome identified cell proliferation, protein synthesis, and cellular death and survival pathways as prominent biological processes. Administration of acrylamide elicited 70 differentially regulated (fold-change =1.5 or = 0.67) genes, several of which were orthogonally validated using quantitative PCR. Pathways that initiate gene and protein synthesis to promote cellular survival were prominent amongst the dysregulated pathways. Inflammation was also a key transcriptomic response to acrylamide, with the cytokine, Colony stimulating factor 2 (Csf2) identified as a top-ranked upstream driver and inflammatory mediator associated with recovery of homeostasis. Early growth response (Egr1), C-C motif chemokine ligand 8 (Ccl8), and Collagen, type V, alpha 1 (Col5a1) were also identified amongst the dysregulated genes. Additionally, acrylamide treatment led to subtle changes in the expression of genes that encode proteins secreted by the seminal vesicle, including the complement regulator, Complement factor b (Cfb). Conclusions: These data add to emerging evidence demonstrating that the seminal vesicles, like other male reproductive tract tissues, are sensitive to environmental insults, and respond in a manner with potential to exert impact on fetal development and later offspring health.
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Nova |
2021 |
Sadeghi N, Tavalaee M, Kiani-Esfahani A, Moazamian A, Gharagozloo P, Aitken RJ, et al., 'Apoptotic M540 bodies present in human semen interfere with flow cytometry-assisted assessment of sperm DNA fragmentation and oxidation', Basic and Clinical Andrology, 31 (2021) [C1]
Background: The use of flow cytometry (FC) to evaluate sperm DNA fragmentation via deoxynucleotidyl transferase terminal fluorescein dUTP nick-end labeling (TUNEL) has shown incon... [more]
Background: The use of flow cytometry (FC) to evaluate sperm DNA fragmentation via deoxynucleotidyl transferase terminal fluorescein dUTP nick-end labeling (TUNEL) has shown inconsistencies compared with conventional fluorescent microscopic analyses. It has been hypothesized that the observed discrepancies could be attributed to the presence of apoptotic bodies that can be labeled with merocyanine 540, the so-called M540 bodies. In order to verify this hypothesis and determine the accuracy of our in-house FC-assisted evaluation of spermatozoa parameters, we used FC to evaluate both the fragmentation of sperm DNA using the TUNEL assay and the oxidation of sperm DNA using the 8-OHdG assay on semen samples with or without M540 bodies. Results: We show that the presence of M540 bodies lead to underestimation of both the level of sperm DNA fragmentation and sperm DNA oxidation when using FC assisted detection systems. We also observed that this situation is particularly pertinent in semen samples classified as abnormal with respect to the routine WHO semen evaluation as they appear to contain more M540 bodies than normal samples. Conclusions: We conclude that M540 bodies interfere with both FC-conducted assays designed to evaluate sperm nuclear/DNA integrity. Exclusion of these contaminants in unprepared semen samples should be performed in order to correctly appreciate the true level of sperm DNA/nuclear damage which is known to be a critical male factor for reproductive success.
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Nova |
2021 |
Aitken RJ, 'COVID-19 and male infertility: An update', ANDROLOGY, 10 8-10 (2021)
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2021 |
Fraser B, Peters AE, Sutherland JM, Liang M, Rebourcet D, Nixon B, Aitken RJ, 'Biocompatible Nanomaterials as an Emerging Technology in Reproductive Health; a Focus on the Male', Frontiers in Physiology, 12 (2021) [C1]
A growing body of research has confirmed that nanoparticle (NP) systems can enhance delivery of therapeutic and imaging agents as well as prevent potentially damaging systemic exp... [more]
A growing body of research has confirmed that nanoparticle (NP) systems can enhance delivery of therapeutic and imaging agents as well as prevent potentially damaging systemic exposure to these agents by modifying the kinetics of their release. With a wide choice of NP materials possessing different properties and surface modification options with unique targeting agents, bespoke nanosystems have been developed for applications varying from cancer therapeutics and genetic modification to cell imaging. Although there remain many challenges for the clinical application of nanoparticles, including toxicity within the reproductive system, some of these may be overcome with the recent development of biodegradable nanoparticles that offer increased biocompatibility. In recognition of this potential, this review seeks to present recent NP research with a focus on the exciting possibilities posed by the application of biocompatible nanomaterials within the fields of male reproductive medicine, health, and research.
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Nova |
2021 |
Gharagozloo P, Cartagena S, Moazamian A, Drevet JR, Somkuti S, Aitken RJ, 'Rapid impact of COVID-19 infection on semen quality: a case report', TRANSLATIONAL ANDROLOGY AND UROLOGY, 11 110-115 (2021)
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2021 |
Arachchige Chamila Samarasinghe SV, Krishnan K, Aitken RJ, Naidu R, Megharaj M, 'Persistence of the parabens in soil and their potential toxicity to earthworms', ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY, 83 (2021) [C1]
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Nova |
2021 |
Ciccone IM, Costa EMF, Pariz JR, Teixeira TA, Drevet JR, Gharagozloo P, et al., 'Serum vitamin D content is associated with semen parameters and serum testosterone levels in men', ASIAN JOURNAL OF ANDROLOGY, 23 52-+ [C1]
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Nova |
2021 |
Naidu R, Biswas B, Willett IR, Cribb J, Kumar Singh B, Paul Nathanail C, et al., 'Chemical pollution: A growing peril and potential catastrophic risk to humanity', Environment International, 156 (2021) [C1]
Anthropogenic chemical pollution has the potential to pose one of the largest environmental threats to humanity, but global understanding of the issue remains fragmented. This art... [more]
Anthropogenic chemical pollution has the potential to pose one of the largest environmental threats to humanity, but global understanding of the issue remains fragmented. This article presents a comprehensive perspective of the threat of chemical pollution to humanity, emphasising male fertility, cognitive health and food security. There are serious gaps in our understanding of the scale of the threat and the risks posed by the dispersal, mixture and recombination of chemicals in the wider environment. Although some pollution control measures exist they are often not being adopted at the rate needed to avoid chronic and acute effects on human health now and in coming decades. There is an urgent need for enhanced global awareness and scientific scrutiny of the overall scale of risk posed by chemical usage, dispersal and disposal.
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Nova |
2021 |
Aitken RJ, 'Antioxidant trials-the need to test for stress', HUMAN REPRODUCTION OPEN, 2021 (2021)
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2021 |
Aitken RJ, 'COVID-19 and human spermatozoa-Potential risks for infertility and sexual transmission?', ANDROLOGY, 9 48-52 (2021) [C1]
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Nova |
2021 |
Zhao F, Whiting S, Lambourne S, Aitken RJ, Sun Y-P, 'Melatonin alleviates heat stress-induced oxidative stress and apoptosis in human spermatozoa', FREE RADICAL BIOLOGY AND MEDICINE, 164 410-416 (2021) [C1]
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Nova |
2020 |
Aitken RJ, Gregoriatis D, Kutzera L, Towney E, Lin M, Wilkins A, Gibb Z, 'Patterns of MTT reduction in mammalian spermatozoa', Reproduction, 160 431-445 (2020) [C1]
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Nova |
2020 |
Gibb Z, Griffin RA, Aitken RJ, De Iuliis GN, 'Functions and effects of reactive oxygen species in male fertility', Animal Reproduction Science, 220 (2020) [C1]
Reactive oxygen species (ROS) are implicated in all aspects of cellular functions. While the importance of ROS as signalling molecules is well described, ROS are also associated w... [more]
Reactive oxygen species (ROS) are implicated in all aspects of cellular functions. While the importance of ROS as signalling molecules is well described, ROS are also associated with stress pathologies. Within the reproduction field, there are associations with reduced fertility as a result of lipid peroxidation, protein dysfunction, premature cell death and DNA damage which readily occur in spermatozoa. These oxidative insults can arise in vivo, or in vitro as a result of sperm storage, purification and processing. Following a brief description of the production, homeostasis and functions of ROS in mammalian sperm function, this review paper will focus on describing the predominant sources of ROS in the ejaculate, the effects of ROS on a cellular and molecular level, and the actions of ROS from the whole animal perspective. There is highlighting of some studies, which have revealed the mechanisms for these observations, along with some strategies to ameliorate or prevent the instigation of the oxidative stress cascade before irreversible damage to spermatozoa occurs.
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2020 |
Aitken RJ, Whiting S, Connaughton H, Curry B, Reinheimer T, van Duin M, 'A novel pathway for the induction of DNA damage in human spermatozoa involving extracellular cell-free DNA', Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 821 (2020) [C1]
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2020 |
Aitken RJ, De Iuliis GN, Nixon B, 'The Sins of Our Forefathers: Paternal Impacts on De Novo Mutation Rate and Development', Annual Review of Genetics, 54 1-24 (2020) [C1]
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2020 |
Griffin RA, Swegen A, Baker M, Aitken RJ, Skerrett-Byrne DA, Rodriguez AS, et al., 'Mass spectrometry reveals distinct proteomic profiles in high- And low-quality stallion spermatozoa', Reproduction, 160 695-707 (2020) [C1]
The horse breeding industry relies upon optimal stallion fertility. Conventional sperm assessments provide limited information regarding ejaculate quality and are not individually... [more]
The horse breeding industry relies upon optimal stallion fertility. Conventional sperm assessments provide limited information regarding ejaculate quality and are not individually predictive of fertilizing potential. The aim of this study was to harness mass spectrometry to compare the proteomic profiles of high- and low-quality stallion spermatozoa, with the ultimate goal of identifying fertility biomarker candidates. Extended stallion semen (n = 12) was fractionated using Percoll density gradients to isolate low-quality and high-quality sperm populations. Motility and morphological assessments were carried out, and proteomic analyses was conducted using UHPLC-MS/MS. High-quality spermatozoa recorded higher total (95.2 ± 0.52% vs 70.6 ± 4.20%; P = 0.001) and progressive motilities (43.4 ± 3.42% vs 27.3 ± 4.32%; P = 0.05), and a higher proportion of morphologically normal cells (50.2 ± 4.34% vs 38.8 ± 2.72%; P = 0.05). In total, 1069 proteins were quantified by UHPLC-MS/MS, of which 22 proteins were significantly more abundant in the high-quality sperm population (P = 0.05). A-kinase anchor protein 4 (AKAP4) and Hexokinase 1 (HK1) were considered possible biomarker candidates and their differential expression was confirmed by immunoblot. Protein expression was significantly correlated with total (AKAP4 R2 = 0.38, P = 0.01; HK1 R2 = 0.46, P = 0.001) and progressive motilities (AKAP4 R2 = 0.51, P = 0.001; HK1 R2 = 0.55, P = 0.01), percentage rapid (AKAP4 R2 = 0.29, P = 0.05; HK1 R2 = 0.58, P = 0.001), straight-line velocity (HK1 R2 = 0.50, P = 0.01) and straightness (HK1 R2 = 0.40, P = 0.01). Furthermore, AKAP4 was highly susceptible to adduction by 4-hydroxynonenal (4HNE), which resulted in a global reduction in the phosphorylation profiles following capacitation. In conclusion, the proteomic profiles of high- and low-quality stallion spermatozoa differ substantially, and proteins such as AKAP4 and HK1 could serve as biomarkers of ejaculate quality.
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2020 |
Aitken RJ, Baker MA, 'The Role of Genetics and Oxidative Stress in the Etiology of Male Infertility-A Unifying Hypothesis?', FRONTIERS IN ENDOCRINOLOGY, 11 (2020) [C1]
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2020 |
Drevet JR, Aitken RJ, 'Oxidation of Sperm Nucleus in Mammals: A Physiological Necessity to Some Extent with Adverse Impacts on Oocyte and Offspring.', Antioxidants, 9 (2020) [C1]
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2020 |
Aitken RJ, Drevet JR, 'The importance of oxidative stress in determining the functionality of mammalian spermatozoa: A two-edged sword', Antioxidants, 9 (2020) [C1]
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2020 |
Aitken RJ, 'Impact of oxidative stress on male and female germ cells: implications for fertility', Reproduction (Cambridge, England), 159 R189-R201 (2020) [C1]
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2020 |
Aitken RJ, Barratt CLR, 'In Memoriam: Lynn R Fraser', ANDROLOGY, 8 532-534 (2020)
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2020 |
Aitken RJ, 'So near yet so far away', F and S Reports, 1 176 (2020)
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2020 |
Aitken RJ, 'The Male Is Significantly Implicated as the Cause of Unexplained Infertility', SEMINARS IN REPRODUCTIVE MEDICINE, 38 3-20 (2020) [C1]
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2020 |
Fraser BA, Miller K, Trigg NA, Smith ND, Western PS, Nixon B, Aitken RJ, 'A novel approach to nonsurgical sterilization; application of menadione-modified gonocyte-targeting M13 bacteriophage for germ cell ablation in utero', PHARMACOLOGY RESEARCH & PERSPECTIVES, 8 (2020) [C1]
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2019 |
Martin JH, Aitken RJ, Bromfield E, Cafe SL, Sutherland JM, Frost ER, et al., 'Investigation into the presence and functional significance of proinsulin C-peptide in the female germline', Biology of Reproduction, 100 1275-1289 (2019) [C1]
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2019 |
Gaikwad AS, Anderson AL, Merriner DJ, O'Connor AE, Houston BJ, Aitken RJ, et al., 'GLIPR1L1 is an IZUMO-binding protein required for optimal fertilization in the mouse.', BMC Biology, 17 (2019) [C1]
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2019 |
Houston BJ, Nixon B, McEwan KE, Martin J, King BV, Aitken RJ, De Iuliis GN, 'Whole-body exposures to radiofrequency-electromagnetic energy can cause DNA damage in mouse spermatozoa via an oxidative mechanism', Scientific Reports, 9 (2019) [C1]
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2019 |
Swegen A, Smith ND, Gibb Z, Curry BJ, Aitken RJ, 'The serine protease testisin is present on the surface of capacitated stallion spermatozoa and interacts with key zona pellucida binding proteins', Andrology, 7 199-212 (2019) [C1]
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2019 |
Youngson NA, Uddin GM, Das A, Martinez C, Connaughton HS, Whiting S, et al., 'Impacts of obesity, maternal obesity and nicotinamide mononucleotide supplementation on sperm quality in mice', Reproduction (Cambridge, England), 158 169-179 (2019) [C1]
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2019 |
Bromfield E, Walters JLH, Cafe S, Bernstein I, Stanger SR, Anderson AL, et al., 'Differential cell death decisions in the testis: evidence for an exclusive window of ferroptosis in round spermatids', Molecular Human Reproduction, 25 241-256 (2019) [C1]
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2019 |
Aitken RJ, Maddern GJ, 'Learning from death', ANZ JOURNAL OF SURGERY, 89 1355-1356 (2019)
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2019 |
Xavier MJ, Roman SD, Aitken RJ, Nixon B, 'Transgenerational inheritance: how impacts to the epigenetic and genetic information of parents affect offspring health', HUMAN REPRODUCTION UPDATE, 25 519-541 (2019) [C1]
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2019 |
Martin JH, Aitken RJ, Bromfield EG, Nixon B, 'DNA damage and repair in the female germline: contributions to ART.', Human Reproduction Update, 25 180-201 (2019) [C1]
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2019 |
Griffin RA, Baker M, Aitken RJ, Swegen A, Gibb Z, 'What makes a fertile sperm? Unique molecular attributes of stallion fertility.', Reproduction (Cambridge, England), 158 R125-R137 (2019) [C1]
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2019 |
Xavier MJ, Nixon B, Roman SD, Scott RJ, Drevet JR, Aitken RJ, 'Paternal impacts on development: Identification of genomic regions vulnerable to oxidative DNA damage in human spermatozoa', Human Reproduction, 34 1876-1890 (2019) [C1]
STUDY QUESTION: Do all regions of the paternal genome within the gamete display equivalent vulnerability to oxidative DNA damage? SUMMARY ANSWER: Oxidative DNA damage is not rando... [more]
STUDY QUESTION: Do all regions of the paternal genome within the gamete display equivalent vulnerability to oxidative DNA damage? SUMMARY ANSWER: Oxidative DNA damage is not randomly distributed in mature human spermatozoa but is instead targeted, with particular chromosomes being especially vulnerable to oxidative stress. WHAT IS KNOWN ALREADY: Oxidative DNA damage is frequently encountered in the spermatozoa of male infertility patients. Such lesions can influence the incidence of de novo mutations in children, yet it remains to be established whether all regions of the sperm genome display equivalent susceptibility to attack by reactive oxygen species. STUDY DESIGN, SIZE, DURATION: Human spermatozoa obtained from normozoospermic males (n = 8) were split into equivalent samples and subjected to either hydrogen peroxide (H2O2) treatment or vehicle controls before extraction of oxidized DNA using a modified DNA immunoprecipitation (MoDIP) protocol. Specific regions of the genome susceptible to oxidative damage were identified by next-generation sequencing and validated in the spermatozoa of normozoospermic males (n = 18) and in patients undergoing infertility evaluation (n = 14). PARTICIPANTS/MATERIALS, SETTING, METHODS: Human spermatozoa were obtained from normozoospermic males and divided into two identical samples prior to being incubated with either H2O2 (5 mm, 1 h) to elicit oxidative stress or an equal volume of vehicle (untreated controls). Alternatively, spermatozoa were obtained from fertility patients assessed as having high basal levels of oxidative stress within their spermatozoa. All semen samples were subjected to MoDIP to selectively isolate oxidized DNA, prior to sequencing of the resultant DNA fragments using a next-generation whole-genomic sequencing platform. Bioinformatic analysis was then employed to identify genomic regions vulnerable to oxidative damage, several of which were selected for real-time quantitative PCR (qPCR) validation. MAIN RESULTS AND THE ROLE OF CHANCE: Approximately 9000 genomic regions, 150-1000 bp in size, were identified as highly vulnerable to oxidative damage in human spermatozoa. Specific chromosomes showed differential susceptibility to damage, with chromosome 15 being particularly sensitive to oxidative attack while the sex chromosomes were protected. Susceptible regions generally lay outside protamine-and histone-packaged domains. Furthermore, we confirmed that these susceptible genomic sites experienced a dramatic (2-15-fold) increase in their burden of oxidative DNA damage in patients undergoing infertility evaluation compared to normal healthy donors. LIMITATIONS, REASONS FOR CAUTION: The limited number of samples analysed in this study warrants external validation, as do the implications of our findings. Selection of male fertility patients was based on high basal levels of oxidative stress within their spermatozoa as opposed to specific sub-classes of male factor infertility. WIDER IMPLICATIONS OF THE FINDINGS: The identification of genomic regions susceptible to oxidation in the male germ line will be of value in focusing future analyses into the mutational load carried by children in response to paternal factors such as age, the treatment of male infertility using ART and paternal exposure to environmental toxicants. STUDY FUNDING/COMPETING INTEREST(S): Project support was provided by the University of Newcastle's (UoN) Priority Research Centre for Reproductive Science. M.J.X. was a recipient of a UoN International Postgraduate Research Scholarship. B.N. is the recipient of a National Health and Medical Research Council of Australia Senior Research Fellowship. Authors declare no conflict of interest.
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2019 |
Lu Y, Oura S, Matsumura T, Oji A, Sakurai N, Fujihara Y, et al., 'CRISPR/Cas9-mediated genome editing reveals 30 testis-enriched genes dispensable for male fertility in mice .', Biology of Reproduction, 101 501-511 (2019) [C1]
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2019 |
Ortega Ferrusola C, Martin Muñoz P, Ortiz-Rodriguez JM, Anel-López L, Balao da Silva C, Álvarez M, et al., 'Depletion of thiols leads to redox deregulation, production of 4-hydroxinonenal and sperm senescence: a possible role for GSH regulation in spermatozoa.', Biology of Reproduction, 100 1090-1107 (2019) [C1]
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2018 |
Xavier MJ, Mitchell LA, McEwan KE, Scott RJ, Aitken RJ, 'Genomic integrity in the male germ line: evidence in support of the disposable soma hypothesis.', Reproduction, 156 269-282 (2018) [C1]
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2018 |
Griffin RA, Swegen A, Aitken JR, Gibb Z, 'Investigation Into Predictors of Stallion Fertility and Associations With Oxidative Damage', Journal of Equine Veterinary Science, 66 63-63 (2018)
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2018 |
Swegen A, Clulow JR, Baker M, Aitken RJ, Tram QT, Gibb Z, 'Unraveling Infertility: Deciphering the Molecular Basis of Idiopathic Infertility in a Thoroughbred Stallion', Journal of Equine Veterinary Science, 66 90-90 (2018)
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2018 |
Lawson EF, Gibb Z, de Ruijter-Villani M, Smith ND, Stout TA, Clutton-Brock A, et al., 'Proteomic Analysis of Pregnant Mare Uterine Fluid', Journal of Equine Veterinary Science, 66 171-172 (2018)
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2018 |
Xavier MJ, Nixon B, Roman SD, Aitken RJ, 'Improved methods of DNA extraction from human spermatozoa that mitigate experimentally-induced oxidative DNA damage', PLOS ONE, 13 (2018) [C1]
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Nova |
2018 |
Aitken RJ, 'Not every sperm is sacred; a perspective on male infertility', MOLECULAR HUMAN REPRODUCTION, 24 287-298 (2018) [C1]
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2018 |
Martin J, Bromfield EG, Aitken RJ, Lord T, Nixon B, 'Double Strand Break DNA Repair occurs via Non-Homologous End-Joining in Mouse MII Oocytes', Scientific Reports, 8 1-15 (2018) [C1]
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2018 |
Aitken RJ, 'Nitroblue tetrazolium (NBT) assay', REPRODUCTIVE BIOMEDICINE ONLINE, 36 90-91 (2018)
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2018 |
Houston BJ, Nixon B, King BV, Aitken RJ, De Iuliis GN, 'Probing the Origins of 1,800 MHz Radio Frequency Electromagnetic Radiation Induced Damage in Mouse Immortalized Germ Cells and Spermatozoa in vitro', FRONTIERS IN PUBLIC HEALTH, 6 (2018) [C1]
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2018 |
Vorilhon S, Brugnon F, Kocer A, Dollet S, Bourgne C, Berger M, et al., 'Accuracy of human sperm DNA oxidation quantification and threshold determination using an 8-OHdG immuno-detection assay', Human Reproduction, 33 553-562 (2018) [C1]
STUDY QUESTION: Can a discriminant threshold be determined for human sperm DNA oxidation? SUMMARY ANSWER: A discriminant threshold was found with 65.8% of 8-hydroxy-2'-deoxyg... [more]
STUDY QUESTION: Can a discriminant threshold be determined for human sperm DNA oxidation? SUMMARY ANSWER: A discriminant threshold was found with 65.8% of 8-hydroxy-2'-deoxyguanosine (8-OHdG)-positive sperm cells and a mean intensity of fluorescence (MIF) of 552 arbitrary units. WHAT IS KNOWN ALREADY: Oxidative stress is known to interfere with sperm quality and fertilizing capacity. However, current practice does not include the routine determination of oxidative DNA damage in spermatozoa; optimized consensus protocols are lacking and no thresholds of normality have been established. STUDY DESIGN, SIZE, DURATION: Intra-and inter-method comparisons between four protocols (IIV) were conducted to determine the most relevant and efficient means of assessing human sperm 8-OHdG content. Tests of assay repeatability, specificity, sensitivity and stability were performed to validate an optimized methodology for routine diagnostic use. PARTICIPANTS/MATERIALS, SETTING, METHODS: This prospective study compared three immuno-detection methods including immunocytochemistry, fluorescence microscopy and flow cytometry. Sperm DNA oxidation for 80 patients was determined relative to semen parameters and clinical conditions, using the selected immuno-detection protocol in comparison with a commercial kit. These patients (age 351 years: mean SEM) presented with normozoospermic (n = 40) or altered parameters (necro-or/and astheno-or/and teratozoospermia or/and leukocytospermia). MAIN RESULTS AND THE ROLE OF CHANCE: Significant positive Pearson and Spearman correlations were determined for 8-OHdG values and sperm parameters using protocol III. A notable high and positive correlation was revealed for MIF with BMI and leukocyte concentration. Protocol III was the most discriminating method regarding assay repeatability, specificity, sensitivity, stability and reliability for sperm parameter alterations, in particular leukocytospermia according to parametric or non-parametric tests, effect-size determinations and factorial analysis such as principal component analysis and factor discriminant analysis. Of interest is that 39% of the subjects with 'pathological' sperm DNA oxidation values were normozoospermic. LIMITATIONS, REASONS FOR CAUTION: The oligozoospermic population was not evaluated in this study because insufficient material was available to carry out the comparisons. However, spermatozoa concentration was taken into account in the statistical analysis. WIDER IMPLICATIONS OF THE FINDINGS: Our study is the first validation of a protocol to determine a discriminant threshold for human sperm DNA oxidation. The protocol's detection accuracy for 8-OHdG human sperm DNA residues, stability over time, and relationship to human sperm quality were demonstrated. The assay should find application in the diagnosis of male factor infertility associated with oxidative stress.
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2018 |
Davis S, Babidge W, Kiermeier A, Aitken R, Maddern G, 'Perioperative Mortality Following Bariatric Surgery in Australia', OBESITY SURGERY, 28 1329-1334 (2018)
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2018 |
Walters JLH, De Iuliis GN, Dun MD, Aitken RJ, McLaughlin EA, Nixon B, Bromfield EG, 'Pharmacological inhibition of arachidonate 15-lipoxygenase protects human spermatozoa against oxidative stress.', Biology of reproduction, 98 784-794 (2018) [C1]
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2018 |
Samarasinghe SVAC, Krishnan K, Naidu R, Megharaj M, Miller K, Fraser B, Aitken RJ, 'Parabens generate reactive oxygen species in human spermatozoa', ANDROLOGY, 6 532-541 (2018) [C1]
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2018 |
Mihalas BP, Bromfield EG, Sutherland JM, De Iuliis GN, McLaughlin EA, John Aitken R, Nixon B, 'Oxidative damage in naturally aged mouse oocytes is exacerbated by dysregulation of proteasomal activity', Journal of Biological Chemistry, 293 18944-18964 (2018) [C1]
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2018 |
O'Brien AP, Hurley J, Linsley P, McNeil K, Fletcher R, Aitken RJ, 'Men s Preconception Health: A Primary Health-Care Viewpoint', American Journal of Men's Health, 12 1575-1581 (2018) [C1]
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2018 |
Houston BJ, Nixon B, Martin JH, De Iuliis GN, Trigg NA, Bromfield EG, et al., 'Heat exposure induces oxidative stress and DNA damage in the male germ line', BIOLOGY OF REPRODUCTION, 98 593-606 (2018) [C1]
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2017 |
Bromfield EG, Aitken RJ, McLaughlin EA, Nixon B, 'Proteolytic degradation of heat shock protein A2 occurs in response to oxidative stress in male germ cells of the mouse', MOLECULAR HUMAN REPRODUCTION, 23 91-105 (2017) [C1]
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2017 |
Hall SE, Nixon B, Aitken RJ, 'Non-surgical sterilisation methods may offer a sustainable solution to feral horse (Equus caballus) overpopulation', Reproduction, Fertility and Development, 29 1655-1666 (2017) [C1]
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2017 |
Aitken RJ, 'Reactive oxygen species as mediators of sperm capacitation and pathological damage', MOLECULAR REPRODUCTION AND DEVELOPMENT, 84 1039-1052 (2017) [C1]
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2017 |
Lu Y, Aitken RJ, Lin M, 'Ultrastructural investigation and in vitro recapitulation of spermatid differentiation in a potential bio-indicator species - The marine invertebrate Galeolaria gemineoa (Polychaeta: Serpulidae)', PLOS ONE, 12 (2017) [C1]
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2017 |
Bromfield EG, Mihalas BP, Dun MD, Aitken RJ, McLaughlin EA, Walters JLH, Nixon B, 'Inhibition of arachidonate 15-lipoxygenase prevents 4-hydroxynonenal-induced proteindamage in male germ cells', Biology of Reproduction, 96 598-609 (2017) [C1]
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2017 |
Aitken RJ, 'DNA damage in human spermatozoa; important contributor to mutagenesis in the offspring', TRANSLATIONAL ANDROLOGY AND UROLOGY, 6 S761-S764 (2017)
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2017 |
Lu Y, Lin M, Aitken RJ, 'Exposure of spermatozoa to dibutyl phthalate induces abnormal embryonic development in a marine invertebrate Galeolaria caespitosa (Polychaeta: Serpulidae)', Aquatic Toxicology, 191 189-200 (2017) [C1]
In this study, we have investigated the impact of dibutyl phthalate (DBP) on early embryogenesis in a sessile marine invertebrate, Galeolaria caespitosa. DBP was found to induce s... [more]
In this study, we have investigated the impact of dibutyl phthalate (DBP) on early embryogenesis in a sessile marine invertebrate, Galeolaria caespitosa. DBP was found to induce sperm dysfunction as well as impaired and defective embryogenesis characterised by a particular pattern of abnormality. Thus, after the first cleavage, one blastomere in these abnormal embryos was able to carry out further mitoses, while the other arrested. Analysis of microtubules, chromosomes and actin filaments demonstrated that the mitotic spindles in the abnormal embryos were irregularly bent, shortened and unable to anchor to the cortex, resulting in the defective segregation of chromosomes. Within the non-dividing blastomeres, karyokinesis was found to continue at a slow pace as indicated by the presence of multiple sets of abnormal mitotic spindles. However, cytokinesis had been disrupted in these arrested cells due to a failure to assemble the contractile actin ring, as a result of which one pole of the embryos remained as one large, undivided cell. DBP was found to suppress the activity of superoxide dismutase in spermatozoa and, in association with this change, DBP-treated cells experienced oxidative stress as indicated by the presence of lipid aldehydes, such as 4-hydroxynonenal (4-HNE) in the sperm acrosome and neck. Adduction of lipid aldehydes at the level of the acrosome would be expected to impede the acrosome reaction and account for the significant decrease in fertilisation rates. 4-HNE generated as a consequence of lipid peroxidation in the sperm neck resulted in alkylation of the sperm centrioles. Such paternally damaged centrioles were inherited by the embryos and disrupted cytoskeletal protein organisation during early cleavage, generating the observed abnormalities in embryonic development. This research emphasises the vulnerability of spermatozoa to oxidative damage and highlights novel potential mechanisms for reproductive toxicity involving the alkylation of subcellular structures in spermatozoa by lipid aldehydes.
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2017 |
Maiti K, Sultana Z, Aitken RJ, Morris J, Park F, Andrew B, et al., 'Evidence that fetal death is associated with placental aging.', American journal of obstetrics and gynecology, 217 441.e1-441.e14 (2017) [C1]
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2017 |
Lin M, Zhang X, Murdoch RN, Aitken RJ, 'Swim-up of tammar wallaby (Macropus eugenii) spermatozoa in Biggers, Whitter and Whittingham (BWW) medium: maximisation of sperm motility, minimisation of impairment of sperm metabolism and induction of sperm hyperactivation', REPRODUCTION FERTILITY AND DEVELOPMENT, 29 345-356 (2017) [C1]
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2017 |
Swegen A, Grupen CG, Gibb Z, Baker M, de Ruijter-Villani M, Smith ND, et al., 'From Peptide Masses to Pregnancy Maintenance: A Comprehensive Proteomic Analysis of The Early Equine Embryo Secretome, Blastocoel Fluid and Capsule.', Proteomics, 17 1-13 (2017) [C1]
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Nova |
2017 |
Lu Y, Aitken RJ, Lin M, 'Detailed analysis of the male reproductive system in a potential bio-indicator species - The marine invertebrate Galeolaria caespitosa (Polychaeta: Serpulidae)', PLOS ONE, 12 (2017) [C1]
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2017 |
Martin JH, Bromfield EG, Aitken RJ, Nixon B, 'Biochemical alterations in the oocyte in support of early embryonic development', CELLULAR AND MOLECULAR LIFE SCIENCES, 74 469-485 (2017) [C1]
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2017 |
Hall SE, Aitken RJ, Nixon B, Smith ND, Gibb Z, 'Electrophilic aldehyde products of lipid peroxidation selectively adduct to heat shock protein 90 and arylsulfatase A in stallion spermatozoa', BIOLOGY OF REPRODUCTION, 96 107-121 (2017) [C1]
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2017 |
Sultana Z, Maiti K, Aitken J, Morris J, Dedman L, Smith R, 'Oxidative stress, placental ageing-related pathologies and adverse pregnancy outcomes', AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY, 77 (2017) [C1]
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2017 |
Harper J, Jackson E, Sermon K, Aitken RJ, Harbottle S, Mocanu E, et al., 'Adjuncts in the IVF laboratory: where is the evidence for 'add-on' interventions?', HUMAN REPRODUCTION, 32 485-491 (2017) [C1]
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2016 |
Martin JH, Bromfield EG, Aitken RJ, Lord T, Nixon B, 'Data on the concentrations of etoposide, PSC833, BAPTA-AM, and cycloheximide that do not compromise the vitality of mature mouse oocytes, parthenogenetically activated and fertilized embryos', DATA IN BRIEF, 8 1215-1220 (2016)
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2016 |
Aitken RJ, 'Oxidative stress and the etiology of male infertility', JOURNAL OF ASSISTED REPRODUCTION AND GENETICS, 33 1691-1692 (2016)
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2016 |
Swegen A, Aitken RJ, 'Prospects for immunocontraception in feral horse population control: exploring novel targets for an equine fertility vaccine', Reproduction, Fertility and Development, 28 853-853 (2016) [C1]
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2016 |
Gibb Z, Aitken RJ, 'The Impact of Sperm Metabolism during In Vitro Storage: The Stallion as a Model.', BioMed research international, 2016 9380609 (2016) [C1]
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2016 |
Aitken RJ, Flanagan HM, Connaughton H, Whiting S, Hedges A, Baker MA, 'Involvement of homocysteine, homocysteine thiolactone, and paraoxonase type 1 (PON-1) in the etiology of defective human sperm function.', Andrology, 4 345-360 (2016) [C1]
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2016 |
Houston B, Nixon B, King BV, De Iuliis GN, Aitken RJ, 'The effects of radiofrequency electromagnetic radiation on sperm function', Reproduction, 152 R263-R276 (2016) [C1]
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2016 |
Bromfield EG, McLaughlin EA, Aitken RJ, Nixon B, 'Heat shock protein member A2 forms a stable complex with angiotensin convertingenzymeand protein disulfide isomerase A6 in human spermatozoa', Molecular Human Reproduction, 22 93-109 (2016) [C1]
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2016 |
Leahy T, Rickard JP, Aitken RJ, De Graaf SP, 'Penicillamine prevents ram sperm agglutination in media that support capacitation', Reproduction, 151 167-177 (2016) [C1]
Ram spermatozoa are difficult to capacitate in vitro. Here we describe a further complication, the unreported phenomenon of headto- head agglutination of ram spermatozoa following... [more]
Ram spermatozoa are difficult to capacitate in vitro. Here we describe a further complication, the unreported phenomenon of headto- head agglutination of ram spermatozoa following dilution in the capacitation medium Tyrodes plus albumin, lactate and pyruvate (TALP). Sperm agglutination is immediate, specific and persistent and is not associated with a loss of motility. Agglutination impedes in vitro sperm handling and analysis. So the objectives of this study were to investigate the cause of sperm agglutination and potential agents which may reduce agglutination. The percentage of non-agglutinated, motile spermatozoa increased when bicarbonate was omitted from complete TALP suggesting that bicarbonate ions stimulate the agglutination process. D-penicillamine (PEN), a nucleophilic thiol, was highly effective at reducing agglutination. The inclusion of 250 µM PEN in TALP reduced the incidence of motile, agglutinated spermatozoa from 76.7±2.7% to 2.8±1.4%. It was then assessed if PEN (1 mM) could be included in existing ram sperm capacitation protocols (TALP +1 mM dibutyryl cAMP, caffeine and theophylline) to produce spermatozoa that were simultaneously capacitated and non-agglutinated. This protocol resulted in a sperm population which displayed high levels of tyrosine phosphorylated proteins and lipid disordered membranes (merocyanine-540) while remaining motile, viable, acrosome-intact and non-agglutinated. In summary, PEN (1 mM) can be included in ram sperm capacitation protocols to reduce sperm agglutination and allow for the in vitro assessment of ram sperm capacitation.
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2016 |
Leahy T, Rickard JP, Aitken RJ, De Graaf SP, 'D-penicillamine prevents ram sperm agglutination by reducing the disulphide bonds of a copper-binding sperm protein', Reproduction, 151 491-500 (2016) [C1]
Head-to-head agglutination of ram spermatozoa is induced by dilution in the Tyrode's capacitation medium with albumin, lactate and pyruvate (TALP) and ameliorated by the addi... [more]
Head-to-head agglutination of ram spermatozoa is induced by dilution in the Tyrode's capacitation medium with albumin, lactate and pyruvate (TALP) and ameliorated by the addition of the thiol D-penicillamine (PEN). To better understand the association and disassociation of ram spermatozoa, we investigated the mechanism of action of PEN in perturbing sperm agglutination. PEN acts as a chelator of heavy metals, an antioxidant and a reducing agent. Chelation is not the main mechanism of action, as the broad-spectrum chelator ethylenediaminetetraacetic acid and the copper-specific chelator bathocuproinedisulfonic acid were inferior anti-agglutination agents compared with PEN. Oxidative stress is also an unlikely mechanism of sperm association, as PEN was significantly more effective in ameliorating agglutination than the antioxidants superoxide dismutase, ascorbic acid, a-tocopherol and catalase. Only the reducing agents cysteine and dl-dithiothreitol displayed similar levels of non-agglutinated spermatozoa at 0 h compared with PEN but were less effective after 3 h of incubation (37 °C). The addition of 10 µM Cu2+ to 250 µM PEN + TALP caused a rapid reversion of the motile sperm population from a non-agglutinated state to an agglutinated state. Other heavy metals (cobalt, iron, manganese and zinc) did not provoke such a strong response. Together, these results indicate that PEN prevents sperm association by the reduction of disulphide bonds on a sperm membrane protein that binds copper. ADAM proteins are possible candidates, as targeted inhibition of the metalloproteinase domain significantly increased the percentage of motile, non-agglutinated spermatozoa (52.0% ± 7.8) compared with TALP alone (10.6% ± 6.1).
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Nova |
2016 |
Martin JH, Nixon B, Lord T, Bromfield EG, Aitken RJ, 'Identification of a key role for permeability glycoprotein in enhancing the cellular defense mechanisms of fertilized oocytes', DEVELOPMENTAL BIOLOGY, 417 63-76 (2016) [C1]
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Nova |
2016 |
Gharagozloo P, Gutierrez-Adan A, Champroux A, Noblanc A, Kocer A, Calle A, et al., 'A novel antioxidant formulation designed to treat male infertility associated with oxidative stress: promising preclinical evidence from animal models', HUMAN REPRODUCTION, 31 252-262 (2016) [C1]
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2016 |
Stanger SJ, Law EA, Jamsai D, O'Bryan MK, Nixon B, McLaughlin EA, et al., 'A novel germ cell protein, SPIF (sperm PKA interacting factor), is essential for the formation of a PKA/TCP11 complex that undergoes conformational and phosphorylation changes upon capacitation', FASEB Journal, 30 2777-2791 (2016) [C1]
Spermatozoa require the process of capacitation to enable them to fertilize an egg. PKA is crucial to capacitation and the development of hyperactivated motility. Sperm PKA is act... [more]
Spermatozoa require the process of capacitation to enable them to fertilize an egg. PKA is crucial to capacitation and the development of hyperactivated motility. Sperm PKA is activated by cAMP generated by the germ cell-enriched adenylyl cyclase encoded by Adcy10. Male mice lacking Adcy10 are sterile, because their spermatozoa are immotile. The current studywas designed to identify binding partners of the sperm-specific (Ca2) catalytic subunit of PKA (PRKACA) by using it as the "bait" in a yeast 2-hybrid system. This approach was used to identify a novel germ cell-enriched protein, sperm PKA interacting factor (SPIF), in 25% of the positive clones. Homozygous Spif-nullmice were embryonically lethal. SPIF was coexpressed and coregulated with PRKACA and with t-complex protein (TCP)-11, a protein associated with PKA signaling. We established that these 3 proteins form part of a novel complex in mouse spermatozoa. Upon capacitation, the SPIF protein becomes tyrosine phosphorylatedin > 95% of sperm. Anapparent molecular rearrangement in the complex occurs, bringing PRKACA and TCP11 into proximity. Taken together, these results suggest a role for the novel complex of SPIF, PRKACA, and TCP11 during spermcapacitation, fertilization, and embryogenesis.
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Nova |
2016 |
Young SAM, Miyata H, Satouh Y, Muto M, Larsen MR, Aitken RJ, et al., 'CRISPR/Cas9-mediated mutation revealed cytoplasmic tail is dispensable for IZUMO1 function and male fertility', REPRODUCTION, 152 665-672 (2016) [C1]
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Nova |
2016 |
Aitken RJ, Muscio L, Whiting S, Connaughton HS, Fraser BA, Nixon B, et al., 'Analysis of the effects of polyphenols on human spermatozoa reveals unexpected impacts on mitochondrial membrane potential, oxidative stress and DNA integrity; implications for assisted reproductive technology', Biochemical Pharmacology, 121 78-96 (2016) [C1]
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Nova |
2016 |
Aitken RJ, Gibb Z, Baker MA, Drevet J, Gharagozloo P, 'Causes and consequences of oxidative stress in spermatozoa', REPRODUCTION FERTILITY AND DEVELOPMENT, 28 1-10 (2016) [C1]
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Nova |
2016 |
Ma L, Qin T, Chu D, Cheng X, Wang J, Wang X, et al., 'Internal Duplications of DH, JH, and C Region Genes Create an Unusual IgH Gene Locus in Cattle', JOURNAL OF IMMUNOLOGY, 196 4358-4366 (2016)
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2016 |
Shirvan AN, Aitken R, 'Isolation of recombinant antibodies directed against surface proteins of Clostridium difficile', BRAZILIAN JOURNAL OF MICROBIOLOGY, 47 394-402 (2016)
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2016 |
Gibb Z, Lambourne SR, Curry BJ, Hall SE, Aitken RJ, 'Aldehyde Dehydrogenase Plays a Pivotal Role in the Maintenance of Stallion Sperm Motility', BIOLOGY OF REPRODUCTION, 94 (2016) [C1]
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Nova |
2016 |
Swegen A, Lambourne SR, Aitken RJ, Gibb Z, 'Rosiglitazone Improves Stallion Sperm Motility, ATP Content, and Mitochondrial Function', BIOLOGY OF REPRODUCTION, 95 (2016) [C1]
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Nova |
2016 |
Young SAM, Miyata H, Satouh Y, Aitken RJ, Baker MA, Ikawa M, 'CABYR is essential for fibrous sheath integrity and progressive motility in mouse spermatozoa', JOURNAL OF CELL SCIENCE, 129 4379-4387 (2016) [C1]
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Nova |
2016 |
Gibb Z, Aitken RJ, 'Recent Developments in Stallion Semen Preservation', JOURNAL OF EQUINE VETERINARY SCIENCE, 43 S29-S36 (2016) [C1]
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Nova |
2015 |
Young SAM, Miyata H, Satouh Y, Kato H, Nozawa K, Isotani A, et al., 'CRISPR/Cas9-mediated rapid generation of multiple mouse lines identified Ccdc63 as essential for spermiogenesis', International Journal of Molecular Sciences, 16 24732-24750 (2015) [C1]
Spermatozoa are flagellated cells whose role in fertilization is dependent on their ability to move towards an oocyte. The structure of the sperm flagella is highly conserved acro... [more]
Spermatozoa are flagellated cells whose role in fertilization is dependent on their ability to move towards an oocyte. The structure of the sperm flagella is highly conserved across species, and much of what is known about this structure is derived from studies utilizing animal models. One group of proteins essential for the movement of the flagella are the dyneins. Using the advanced technology of CRISPR/Cas9 we have targeted three dynein group members; Dnaic1, Wdr63 and Ccdc63 in mice. All three of these genes are expressed strongly in the testis. We generated mice with amino acid substitutions in Dnaic1 to analyze two specific phosphorylation events at S124 and S127, and generated simple knockouts of Wdr63 and Ccdc63. We found that the targeted phosphorylation sites in Dnaic1 were not essential for male fertility. Similarly, Wdr63 was not essential for male fertility; however, Ccdc63 removal resulted in sterile male mice due to shortened flagella. This study demonstrates the versatility of the CRISPR/Cas9 system to generate animal models of a highly complex system by introducing point mutations and simple knockouts in a fast and efficient manner.
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2015 |
Lord T, Martin JH, Aitken RJ, 'Accumulation of Electrophilic Aldehydes During Postovulatory Aging of Mouse Oocytes Causes Reduced Fertility, Oxidative Stress, and Apoptosis', BIOLOGY OF REPRODUCTION, 92 (2015) [C1]
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2015 |
Polhemus A, Moazamian R, connaughton H, fraser BA, Aitken RJ, 'Cytotoxic effects of membrane lipid peroxidation products on human spermatozoa', Molecular Human Reproduction, 10 (2015) |
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2015 |
Varner DD, Gibb Z, Aitken RJ, 'Stallion fertility: A focus on the spermatozoon', Equine Veterinary Journal, 47 16-24 (2015) [C1]
Stallion fertility is a vast subject, with a wide array of permutations that can impact reproductive performance in either positive or negative ways. This review is intended to ad... [more]
Stallion fertility is a vast subject, with a wide array of permutations that can impact reproductive performance in either positive or negative ways. This review is intended to address a mere segment of the male fertility issue, but the very essence of the male contribution to fertilisation, that of the spermatozoon. Spermatozoal ultrastructure and form-to-function are detailed and spermatozoal metabolism is discussed, with specific reference to distinctive characteristics of stallion spermatozoa. Lastly, methods for assessment of spermatozoal function are considered, with emphasis on spermatozoal motility, the acrosome reaction and spermatozoon-oocyte interactions. Closing comments address the need for development and standardisation of molecular-based assays for use with spermatozoa of stallions whose subfertility cannot be explained with conventional tests.
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2015 |
Tanphaichitr N, Kongmanas K, Kruevaisayawan H, Saewu A, Sugeng C, Fernandes J, et al., 'Remodeling of the plasma membrane in preparation for sperm-egg recognition: roles of acrosomal proteins', ASIAN JOURNAL OF ANDROLOGY, 17 574-582 (2015) [C1]
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2015 |
Young SAM, Aitken RJ, Ikawa M, 'Advantages of using the CRISPR/Cas9 system of genome editing to investigate male reproductive mechanisms using mouse models', ASIAN JOURNAL OF ANDROLOGY, 17 623-627 (2015) [C1]
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Nova |
2015 |
Swegen A, Curry BJ, Gibb Z, Lambourne SR, Smith ND, Aitken RJ, 'Investigation of the stallion sperm proteome by mass spectrometry', Reproduction, 149 235-244 (2015) [C1]
Stallion spermatozoa continue to present scientific and clinical challenges with regard to the biological mechanisms responsible for their survival and function. In particular, de... [more]
Stallion spermatozoa continue to present scientific and clinical challenges with regard to the biological mechanisms responsible for their survival and function. In particular, deeper understanding of sperm energy metabolism, defence against oxidative damage and cell-cell interactions should improve fertility assessment and the application of advanced reproductive technologies in the equine species. In this study, we used highly sensitive LC-MS/MS technology and sequence database analysis to identify and characterise the proteome of Percoll-isolated ejaculated equine spermatozoa, with the aim offurthering our understanding of this cell's complex biological machinery. We were able to identify 9883 peptides comprising 1030 proteins, which were subsequently attributed to 975 gene products. Gene ontology analysis for molecular and cellular processes revealed new information about the metabolism, antioxidant defences and receptors of stallion spermatozoa. Mitochondrial proteins and those involved in catabolic processes constituted dominant categories. Several enzymes specific to ß-oxidation of fatty acids were identified, and further experiments were carried out to ascertain their functional significance. Inhibition of carnitine palmitoyl transferase 1, a rate-limiting enzyme of ß-oxidation, reduced motility parameters, indicating that b-oxidation contributes to maintenance of motility in stallion spermatozoa.
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Nova |
2015 |
Purdey MS, Connaughton HS, Whiting S, Schartner EP, Monro TM, Thompson JG, et al., 'Boronate probes for the detection of hydrogen peroxide release from human spermatozoa', Free Radical Biology and Medicine, 81 69-76 (2015) [C1]
Human spermatozoa are compromised by production of reactive oxygen species (ROS), and detection of ROS in spermatozoa is important for the diagnosis of male infertility. The probe... [more]
Human spermatozoa are compromised by production of reactive oxygen species (ROS), and detection of ROS in spermatozoa is important for the diagnosis of male infertility. The probes 2',7'-dichlorohydrofluorescein diacetate (DCFH), dihydroethidium (DHE), and MitoSOX red (MSR) are commonly used for detecting ROS by flow cytometry; however, these probes lack sensitivity to hydrogen peroxide (H2O2), which is particularly damaging to mammalian sperm cells. This study reports the synthesis and use of three aryl boronate probes, peroxyfluor-1 (PF1), carboxyperoxyfluor-1, and a novel probe, 2-(2-ethoxyethoxy)ethoxyperoxyfluor-1 (EEPF1), in human spermatozoa. PF1 and EEPF1 were effective at detecting H2O2 and peroxynitrite (ONOO-) produced by spermatozoa when stimulated with menadione or 4-hydroxynonenal. EEPF1 was more effective at detection of ROS in spermatozoa than DCFH, DHE, or MSR; furthermore it distinguished poorly motile sperm as shown by greater ROS production. EEPF1 should therefore have a significant role in the diagnosis of oxidative stress in male infertility, cryopreservation, age, lifestyle, and exposure to environmental toxicants.
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Nova |
2015 |
Gibb Z, Lambourne SR, Quadrelli J, Smith ND, Aitken RJ, 'L-carnitine and pyruvate are prosurvival factors during the storage of stallion spermatozoa at room temperature', Biology of Reproduction, 93 (2015) [C1]
The spermatozoa of many stallions do not tolerate being cooled, restricting the commercial viability of these animals and necessitating the development of a chemically defined roo... [more]
The spermatozoa of many stallions do not tolerate being cooled, restricting the commercial viability of these animals and necessitating the development of a chemically defined room temperature (RT) storage medium. This study examined the impact of two major modulators of oxidative phosphorylation, pyruvate (Pyr) and L-carnitine (L-C), on the storage of stallion spermatozoa at RT. Optimal concentrations of Pyr (10 mM) and L-C (50 mM) were first identified and these concentrations were then used to investigate the effects of these compounds on sperm functionality and oxidative stress at RT. Mitochondrial and cytosolic reactive oxygen species, along with lipid peroxidation, were all significantly suppressed by the addition of L-C (48 h MitoSOX Red negative: 46.2% vs. 26.1%; 48 and 72 h dihydroethidium negative: 61.6% vs. 43.1% and 64.4% vs. 46.9%, respectively; 48 and 72 h 4-hydroxynonenal negative: 37.1% vs. 23.8% and 41.6% vs. 25.7%, respectively), while the Pyr + L-C combination resulted in significantly higher motility compared to the control at 72 h (total motility: 64.2% vs. 39.4%; progressive motility: 34.2% vs. 15.2%). In addition, supplementation with L-C significantly reduced oxidative DNA damage at 72 h (9.0% vs. 15.6%). To investigate the effects of LC as an osmolyte, comparisons were made between media that were osmotically balanced with NaCl, choline chloride, or L-C. This analysis demonstrated that spermatozoa stored in the L-C balanced medium had significantly higher total motility (55.0% vs. 39.0%), rapid motility (44.0% vs. 25.7%), and ATP levels (70.9 vs. 12.8 ng/ml) following storage compared with the NaCl treatment, while choline chloride did not significantly improve these parameters compared to the control. Finally, mass spectrometry was used to demonstrate that a combination of Pyr and L-C produced significantly higher acetyl-L-carnitine production than any other treatment (6.7 pg/106 spermatozoa vs. control at 4.0 pg/106 spermatozoa). These findings suggest that Pyr and L-C could form the basis of a novel, effective RT storage medium for equine spermatozoa.
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Nova |
2015 |
Leach M, Aitken RJ, Sacks G, 'Sperm DNA fragmentation abnormalities in men from couples with a history of recurrent miscarriage', Australian and New Zealand Journal of Obstetrics and Gynaecology, 55 379-383 (2015) [C1]
Background Previous studies have described an association between sperm with DNA damage and a history of recurrent miscarriage (RM), although it is not clear whether there is bene... [more]
Background Previous studies have described an association between sperm with DNA damage and a history of recurrent miscarriage (RM), although it is not clear whether there is benefit in screening for sperm DNA fragmentation and to what extent DNA fragmentation impacts upon RM. Aims To identify what proportion of couples experiencing RM are affected by DNA fragmentation abnormalities. Materials and Methods In this retrospective study, between 2008 and 2013, couples with a history of recurrent miscarriage (=3 first trimester miscarriages) were investigated comprehensively for known causes (karyotype, uterine, antiphospholipid syndrome, thrombophilia) and also by semen analysis, including DNA fragmentation [sperm chromatin structure analysis (SCSA)]. Statistical analysis was performed on SPSS software with significance taken as P < 0.05. Results There were 108 couples with a median sperm DNA fragmentation index (DFI) of 9.50%. Normal levels were found in 70.5% of men (DFI < 15%), 23% had high levels (DFI 15-30%), and 6.5% had very high levels (DFI > 30%). Couples with otherwise unexplained recurrent miscarriage had significantly higher DFI than those with other causes identified on routine screening (P = 0.012). Conclusions In couples experiencing RM, 30% (32/108) of men had sperm with high levels of DNA fragmentation (DFI > 15%). This may be a contributing factor to the clinical syndrome of RM, and future clinical trials of therapies for these couples are warranted.
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Nova |
2015 |
Moazamian R, Polhemus A, Connaughton H, Fraser B, Whiting S, Gharagozloo P, Aitken RJ, 'Oxidative stress and human spermatozoa: Diagnostic and functional significance of aldehydes generated as a result of lipid peroxidation', Molecular Human Reproduction, 21 502-515 (2015) [C1]
Oxidative stress is known to compromise human sperm function and to activate the intrinsic apoptotic cascade in these cells.One of the key features of oxidatively stressed spermat... [more]
Oxidative stress is known to compromise human sperm function and to activate the intrinsic apoptotic cascade in these cells.One of the key features of oxidatively stressed spermatozoa is the induction of a lipid peroxidation process that results in the formation of aldehydes potentially capable of disrupting sperm function through the formation of adducts with DNA and key proteins. In this study, we have examined the impact of a range of small molecular mass aldehydes generated as a consequence of lipid peroxidation on human sperm function and also compared the two most commonly formed compounds, 4-hydroxynonenal (4HNE) and malondialdehyde (MDA), for their relative ability to reflect a state of oxidative stress in these cells. Dramatic differences in the bioactivity of individual aldehydes were observed, that generally correlated with the second order rate constants describing their interaction with the model nucleophile, glutathione. Our results demonstrate that acrolein and 4HNE were the most reactive lipid aldehydes, inhibiting sperm motility while augmenting reactive oxygen species production, lipid peroxidation, oxidative DNA damage and caspase activation, in a dose-dependent manner (P < 0.001). In contrast, a variety of saturated aldehydes and the well-known marker of oxidative stress, MDA, were without effect on this cell type. While MDA was not cytotoxic per se, its generation did reflect the induction of oxidative stress in vivo and in vitro in a manner that was highly correlated with the bioactive lipid aldehyde, 4HNE. Despite such overall correlations, individual patient samples were observed in which either MDA or 4HNE predominated. Given the relative cytotoxicity of 4HNE, we propose that this aldehyde should be the preferred criterion for diagnosing oxidative stress in the male germ line.
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2015 |
Bromfield E, Aitken RJ, Nixon B, 'Novel characterization of the HSPA2-stabilizing protein BAG6 in human spermatozoa', Molecular Human Reproduction, 21 755-769 (2015) [C1]
While a large cohort of sperm surface receptors underpin sperm-oocyte adhesion processes, our recent work has revealed that the molecular chaperone Heat Shock Protein A2 (HSPA2) i... [more]
While a large cohort of sperm surface receptors underpin sperm-oocyte adhesion processes, our recent work has revealed that the molecular chaperone Heat Shock Protein A2 (HSPA2) is a key regulator of zona pellucida-receptor complex assembly in our own species. Indeed, in the infertile population, spermatozoa that fail to interact with the zona pellucida of the oocyte consistently lack HSPA2 protein expression. While the mechanisms behind this protein deficiency are under consideration, BCL2-associated athanogene 6 (BAG6) has been identified as a key regulator of HSPA2 stability in mouse germ cells. However, in the human, the presence of BAG family proteins remains completely uncharacterized. Consequently, this study aimed to determine the presence of BAG6 in human sperm cells and to characterize its putative interaction with HSPA2 throughout sperm cell development. BAG6 was shown to co-localize with HSPA2 in human testicular germ cells and epididymal spermatozoa. Similarly, BAG6 was identified in the equatorial region of non-capacitated spermatozoa but underwent a marked relocation to the anterior region of the head upon the induction of capacitation in these cells. Protein-protein interaction assays revealed the stable interaction of BAG6 and HSPA2 proteins in mature spermatozoa. Furthermore, examination of the spermatozoa of infertile men with zona pellucida binding defects, related to a lack of HSPA2, revealed a concomitant deficiency in BAG6 protein expression. In view of the findings described in this study, we propose that BAG6 is likely a key regulator of HSPA2 stability/function in human germ cells. Moreover, its under-representation in spermatozoa with zona pellucida binding deficiency suggests that BAG6 may be an important candidate to study for a further understanding of male idiopathic infertility.
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2015 |
Kongmanas K, Kruevaisayawan H, Saewu A, Sugeng C, Fernandes J, Souda P, et al., 'Proteomic characterization of pig sperm anterior head plasma membrane reveals roles of acrosomal proteins in ZP3 binding', Journal of Cellular Physiology, 230 449-463 (2015) [C1]
The sperm anterior head plasma membrane (APM) is the site where sperm first bind to the zona pellucida (ZP). This binding reaches the maximum following the sperm capacitation proc... [more]
The sperm anterior head plasma membrane (APM) is the site where sperm first bind to the zona pellucida (ZP). This binding reaches the maximum following the sperm capacitation process. To gain a better understanding of the sperm-ZP binding mechanisms, we compared protein profiles obtained from mass spectrometry of APM vesicles isolated from non-capacitated and capacitated sperm. The results revealed that ZP-binding proteins were the most abundant group of proteins, with a number of them showing increased levels in capacitated sperm. Blue native gel electrophoresis and far-western blotting revealed presence of high molecular weight (HMW) protein complexes in APM vesicles of both non-capacitated and capacitated sperm, but the complexes (~750-1300kDa) from capacitated sperm possessed much higher binding capacity to pig ZP3 glycoprotein. Proteomic analyses indicated that a number of proteins known for their acrosome localization, including zonadhesin, proacrosin/acrosin and ACRBP, were components of capacitated APM HMW complexes, with zonadhesin being the most enriched protein. Our immunofluorescence results further demonstrated that a fraction of these acrosomal proteins was transported to the surface of live acrosome-intact sperm during capacitation. Co-immunoprecipitation indicated that zonadhesin, proacrosin/acrosin and ACRBP interacted with each other and they may traffic as a complex from the acrosome to the sperm surface. Finally, the significance of zonadhesin in the binding of APM HMW complexes to pig ZP3 was demonstrated; the binding ability was decreased following treatment of the complexes with anti-zonadhesin antibody. Our results suggested that acrosomal proteins, especially zonadhesin, played roles in the initial sperm-ZP binding during capacitation.
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Nova |
2015 |
Gibb Z, Lambourne SR, Quadrelli J, Smith ND, Aitken RJ, 'L-carnitine and pyruvate are prosurvival factors during the storage of stallion spermatozoa at room temperature.', Biology of reproduction, 93 104 (2015)
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2015 |
Aitken JB, Naumovski N, Curry B, Grupen CG, Gibb Z, Aitken RJ, 'Characterization of an L-amino acid oxidase in equine spermatozoa.', Biol Reprod, 92 125 (2015) [C1]
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2015 |
Aitken RJ, Cummins JM, Nixon B, 'The 12 (th) International Symposium on Spermatology.', Asian J Androl, 17 519-520 (2015) [C3]
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2015 |
Aitken RJ, Baker MA, Nixon B, 'Are sperm capacitation and apoptosis the opposite ends of a continuum driven by oxidative stress?', Asian J Androl, 17 633-639 (2015) [C1]
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Nova |
2015 |
Young SAM, Aitken J, Baker MA, 'Phosphorylation of Izumo1 and its role in male infertility', ASIAN JOURNAL OF ANDROLOGY, 17 708-710 (2015) [C1]
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2015 |
Aitken RJ, 'Lessons learned in Andrology: Revelations on a road less traveled', Andrology, 3 805-808 (2015) [C3]
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2015 |
Zhang Y, Leu Y-R, Aitken RJ, Riediker M, 'Inventory of Engineered Nanoparticle-Containing Consumer Products Available in the Singapore Retail Market and Likelihood of Release into the Aquatic Environment', INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH, 12 8717-8743 (2015)
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2015 |
George S, Ho SS, Wong ESP, Tan TTY, Verma NK, Aitken RJ, et al., 'The multi-facets of sustainable nanotechnology - Lessons from a nanosafety symposium', NANOTOXICOLOGY, 9 404-406 (2015)
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2015 |
Reid AT, Anderson AL, Roman SD, McLaughlin EA, McCluskey A, Robinson PJ, et al., 'Glycogen synthase kinase 3 regulates acrosomal exocytosis in mouse spermatozoa via dynamin phosphorylation', FASEB JOURNAL, 29 2872-2882 (2015) [C1]
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2015 |
Lord T, Aitken RJ, 'Fertilization stimulates 8-hydroxy-2'-deoxyguanosine repair and antioxidant activity to prevent mutagenesis in the embryo', Developmental Biology, 406 1-13 (2015) [C1]
Oxidative DNA damage harbored by both spermatozoa and oocytes at the time of fertilization must be repaired prior to S-phase of the first mitotic division to reduce the risk of tr... [more]
Oxidative DNA damage harbored by both spermatozoa and oocytes at the time of fertilization must be repaired prior to S-phase of the first mitotic division to reduce the risk of transversion mutations occurring in the zygote and subverting the normal patterns of cell differentiation and development. Of the characterised oxidative DNA lesions, 8-hydroxy-2'-deoxyguanosine (8OHdG) is particularly mutagenic. The current study reveals for the first time a marked acceleration of 8OHdG repair in the mouse oocyte/zygote by the base excision repair (BER) pathway following fertilization. Specifically, fertilization initiates post-translational modification to BER enzymes such as OGG1 and XRCC1, causing nuclear localisation and accelerated 8OHdG excision. Additionally, both the nuclear and mitochondrial genomes appear to benefit from increased protection against further 8OHdG formation by a fertilization-associated increase in glutathione peroxidase activity. The major limitation of the characterised 8OHdG repair system is the relatively low level of OGG1 expression in the oocyte, in contrast to the male germ line where it is the only constituent of the BER pathway. The male and female germ lines therefore collaborate in the repair of oxidative DNA damage, and oocytes are vulnerable to high levels of 8OHdG being carried into the zygote by the fertilizing spermatozoon.
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2015 |
Kocer A, Henry-Berger J, Noblanc A, Champroux A, Pogorelcnik R, Guiton R, et al., 'Oxidative DNA damage in mouse sperm chromosomes: Size matters', Free Radical Biology and Medicine, 89 993-1002 (2015) [C1]
Normal embryo and foetal development as well as the health of the progeny are mostly dependent on gamete nuclear integrity. In the present study, in order to characterize more pre... [more]
Normal embryo and foetal development as well as the health of the progeny are mostly dependent on gamete nuclear integrity. In the present study, in order to characterize more precisely oxidative DNA damage in mouse sperm we used two mouse models that display high levels of sperm oxidative DNA damage, a common alteration encountered both in in vivo and in vitro reproduction. Immunoprecipitation of oxidized sperm DNA coupled to deep sequencing showed that mouse chromosomes may be largely affected by oxidative alterations. We show that the vulnerability of chromosomes to oxidative attack inversely correlated with their size and was not linked to their GC richness. It was neither correlated with the chromosome content in persisting nucleosomes nor associated with methylated sequences. A strong correlation was found between oxidized sequences and sequences rich in short interspersed repeat elements (SINEs). Chromosome position in the sperm nucleus as revealed by fluorescent in situ hybridization appears to be a confounder. These data map for the first time fragile mouse sperm chromosomal regions when facing oxidative damage that may challenge the repair mechanisms of the oocyte post-fertilization.
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2015 |
Bromfield EG, Aitken RJ, Anderson AL, McLaughlin EA, Nixon B, 'The impact of oxidative stress on chaperone-mediated human sperm-egg interaction.', Hum Reprod, 30 2597-2613 (2015) [C1]
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Nova |
2015 |
Houston B, Curry B, Aitken RJ, 'Human spermatozoa possess an IL4I1 L-amino acid oxidase with a potential role in sperm function', Reproduction, 149 587-596 (2015) [C1]
Reactive oxygen species (ROS) are known to play an important role in the regulation of human sperm function. In this study, we demonstrate for the first time that human spermatozo... [more]
Reactive oxygen species (ROS) are known to play an important role in the regulation of human sperm function. In this study, we demonstrate for the first time that human spermatozoa possess interleukin-induced gene 1 (IL4I1), an L-amino acid oxidase (LAAO) which is capable of generating ROS on exposure to aromatic amino acids in the presence of oxygen. The preferred substrates were found to be phenylalanine and tryptophan while the enzyme was located in the acrosomal region and midpiece of these cells. In contrast to equine and bovine spermatozoa, enzyme activity was lost as soon as the spermatozoa became non-viable. On a cell-to-cell basis human spermatozoa were also shown to generate lower levels of hydrogen peroxide than their equine counterparts on exposure to phenylalanine. Stimulation of LAAO activity resulted in the induction of several hallmarks of capacitation including tyrosine phosphorylation of the sperm flagellum and concomitant activation of phospho-SRC expression. In addition, stimulation of LAAO resulted in an increase in the levels of acrosomal exocytosis in both the presence and absence of progesterone stimulation, via mechanisms that could be significantly reversed by the presence of catalase. As is often the case with free radical-mediated phenomena, prolonged exposure of human spermatozoa to phenylalanine resulted in the stimulation of apoptosis as indicated by significant increases in mitochondrial superoxide generation and the activation of intracellular caspases. These results confirm the existence of an LAAO in human spermatozoa with a potential role in driving the redox regulation of sperm capacitation and acrosomal exocytosis.
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Nova |
2015 |
Nixon B, Bromfield EG, Dun MD, Redgrove KA, McLaughlin EA, Aitken RJ, 'The role of the molecular chaperone heat shock protein A2 (HSPA2) in regulating human sperm-egg recognition', ASIAN JOURNAL OF ANDROLOGY, 17 568-573 (2015) [C1]
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Nova |
2014 |
Gibb Z, Lambourne SR, Aitken RJ, 'The paradoxical relationship between stallion fertility and oxidative stress.', Biology of Reproduction, 91 1-10 (2014) [C1]
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Nova |
2014 |
McPherson NO, Fullston T, Aitken RJ, Lane M, 'Paternal obesity, interventions, and mechanistic pathways to impaired health in offspring', Annals of Nutrition and Metabolism, 64 231-238 (2014) [C1]
Background: The global rates of male overweight/obesity are rising, approaching 70% of the total adult population in Western nations. Overweight/obesity increases the risk of chro... [more]
Background: The global rates of male overweight/obesity are rising, approaching 70% of the total adult population in Western nations. Overweight/obesity increases the risk of chronic diseases; however, there is increasing awareness that male obesity negatively impacts fertility, subsequent pregnancy, and the offspring health burden. Developmental programming is well defined in mothers; however, it is becoming increasingly evident that developmental programming can be paternally initiated and mediated through paternal obesity. Key Messages: Both human and rodent models have established that paternal obesity impairs sex hormones, basic sperm function, and molecular composition. This results in perturbed embryo development and health and an increased subsequent offspring disease burden in both sexes. The reversibility of obesity-induced parental programming has only recently received attention. Promising results in animal models utilizing diet and exercise interventions have shown improvements in sperm function and molecular composition, resulting in restorations of both embryo and fetal health and subsequent male offspring fertility. The direct mode for paternal inheritance is likely mediated via spermatozoa. We propose two main theories for the origin of male obesity-induced paternal programming: (1) accumulation of sperm DNA damage resulting in de novo mutations in the embryo and (2) changes in sperm epigenetic marks (microRNA, methylation, or acetylation) altering the access, transcription, and translation of paternally derived genes during early embryogenesis. Conclusions: Paternal overweight/obesity induces paternal programming of offspring phenotypes likely mediated through genetic and epigenetic changes in spermatozoa. These programmed changes to offspring health appear to be partially restored via diet/exercise interventions in obese fathers preconception, which have been shown to improve aspects of sperm DNA integrity. However, the majority of data surrounding paternal obesity and offspring phenotypes have come from rodent models; therefore, we contend that it will be increasingly important to study population-based data to determine the likely mode of inheritance in humans.
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Nova |
2014 |
Aitken RJ, Finnie JM, Muscio L, Whiting S, Connaughton HS, Kuczera L, et al., 'Potential importance of transition metals in the induction of DNA damage by sperm preparation media', Human Reproduction, 29 2136-2147 (2014) [C1]
STUDY QUESTION: What are the mechanisms by which the preparation of spermatozoa on discontinuous density gradients leads to an increase in oxidative DNA damage? SUMMARY ANSWER: Th... [more]
STUDY QUESTION: What are the mechanisms by which the preparation of spermatozoa on discontinuous density gradients leads to an increase in oxidative DNA damage? SUMMARY ANSWER: The colloidal silicon solutions that are commonly used to prepare human spermatozoa for assisted reproduction technology (ART) purposes contain metals in concentrations that promote free radical-mediated DNA damage. WHAT IS KNOWN ALREADY: Sporadic reports have already appeared indicating that the use of colloidal silicon-based discontinuous density gradients for sperm preparation is occasionally associated with the induction of oxidative DNA damage. The cause of this damage is however unknown. STUDY DESIGN, SIZE, DURATION: This study comprised a series of experiments designed to: (i) confirm the induction of oxidativeDNA damage in spermatozoa prepared on commercially available colloidal silicon gradients, (ii) compare the levels of damage observed with alterative sperm preparation techniques including an electrophoretic approach and (iii) determine the cause of the oxidative DNA damage and develop strategies for its prevention. The semen samples employed for this analysis involved a cohort of >50 unselected donors and at least three independent samples were used for each component of the analysis. PARTICIPANTS/MATERIALS, SETTING, METHODS: The setting was a University biomedical science laboratory. The major techniques employed were: (i) flow cytometry to study reactive oxygen species generation, lipid peroxidation and DNA damage, (ii) computeraided sperm analysis to measure spermmovement and (iii) inductively coupled mass spectrometry to determine the elemental composition of sperm preparation media. MAIN RESULTS AND THE ROLE OF CHANCE: Oxidative DNA damage is induced in spermatozoa prepared on PureSperm® discontinuous colloidal silicon gradients (P < 0.001 versus repeated centrifugation) because this medium contains metals, particularly Fe, Al and Cu, which are known to promote free radical generation in the immediate vicinity of DNA. This damage can be significantly accentuated by reducing agents, such as ascorbate (P < 0.001) and inhibited by selective chelation (P < 0.001). This problem is not confined to PureSperm®; analysis of additional commercial sperm preparation media revealed that metal contamination is a relatively constant feature of such products. LIMITATIONS, REASONS FOR CAUTION: While the presence of metals, particularly transition metals, may exacerbate the levels of oxidative DNA damage seen in human spermatozoa, the significance of such damage has not yet been tested in suitably powered clinical trials. WIDER IMPLICATIONS OF THE FINDINGS: The results explain why the preparation of spermatozoa on discontinuous colloidal silicon gradients can result in oxidative DNA damage. The results are of immediate relevance to the development of safe, effective protocols for the preparation of spermatozoa for ART purposes. STUDY FUNDING/COMPETING INTEREST(S): The studywas funded by the Australian Health and Medical ResearchCouncil. One of the authors (R.J.A.) has had a consultantship with a biotechnology company, NuSep, interested in the development of electrophoretic methods of sperm preparation. He has no current financial interest in this area. None of the other authors have a conflict of interest to declare.
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Nova |
2014 |
Aitken RJ, Lambourne S, Gibb Z, 'The John Hughes Memorial Lecture: Aspects of Sperm Physiology-Oxidative Stress and the Functionality of Stallion Spermatozoa', JOURNAL OF EQUINE VETERINARY SCIENCE, 34 17-27 (2014) [C1]
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Nova |
2014 |
Aitken RJ, Smith TB, Jobling MS, Baker MA, De Iuliis GN, 'Oxidative stress and male reproductive health', Asian Journal of Andrology, 16 31-38 (2014) [C1]
One of the major causes of defective sperm function is oxidative stress, which not only disrupts the integrity of sperm DNA but also limits the fertilizing potential of these cell... [more]
One of the major causes of defective sperm function is oxidative stress, which not only disrupts the integrity of sperm DNA but also limits the fertilizing potential of these cells as a result of collateral damage to proteins and lipids in the sperm plasma membrane. The origins of such oxidative stress appear to involve the sperm mitochondria, which have a tendency to generate high levels of superoxide anion as a prelude to entering the intrinsic apoptotic cascade. Unfortunately, these cells have very little capacity to respond to such an attack because they only possess the first enzyme in the base excision repair (BER) pathway, 8-oxoguanine glycosylase 1 (OGG1). The latter successfully creates an abasic site, but the spermatozoa cannot process the oxidative lesion further because they lack the downstream proteins (APE1, XRCC1) needed to complete the repair process. It is the responsibility of the oocyte to continue the BER pathway prior to initiation of S-phase of the first mitotic division. If a mistake is made by the oocyte at this stage of development, a mutation will be created that will be represented in every cell in the body. Such mechanisms may explain the increase in childhood cancers and other diseases observed in the offspring of males who have suffered oxidative stress in their germ line as a consequence of age, environmental or lifestyle factors. The high prevalence of oxidative DNA damage in the spermatozoa of male infertility patients may have implications for the health of children conceivedin vitro and serves as a driver for current research into the origins of free radical generation in the germ line. © 2014 AJA, SIMM & SJTU. All rights reserved.
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Nova |
2014 |
Aitken RJ, 'Age, the environment and our reproductive future: bonking baby boomers and the future of sex', REPRODUCTION, 147 S1-S11 (2014) [C1]
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Nova |
2014 |
Bromfield EG, Aitken RJ, Gibb Z, Lambourne SR, Nixon B, 'Capacitation in the presence of methyl-beta-cyclodextrin results in enhanced zona pellucida-binding ability of stallion spermatozoa', REPRODUCTION, 147 153-166 (2014) [C1]
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Nova |
2013 |
Aitken RJ, 'Human spermatozoa: Revelations on the road to conception', F1000Prime Reports, 5 (2013) [C2]
Human spermatozoa are highly complex specialized cells designed to survive a long and perilous journey from the site of insemination to the upper reaches of the female reproductiv... [more]
Human spermatozoa are highly complex specialized cells designed to survive a long and perilous journey from the site of insemination to the upper reaches of the female reproductive tract where fertilization occurs. During this journey, these cells have to run the gauntlet laid down by the female immune system and time their physiological maturation so that as soon as an egg appears in the Fallopian tube, they are equipped to recognize this cell and participate in a remarkable cascade of cellular interactions culminating in fertilization. Despite their high level of specialization, human spermatozoa are notoriously inadequate and appear to be major contributors to the poor fertility that characterizes our species. Defective spermatozoa are also known to have a major impact on the progress of pregnancy and the health trajectory of the offspring, resulting in paternally mediated increases in miscarriage rate and a range of diseases in the progeny, including dominant genetic diseases and cancer. The causes of defective sperm function are complex and involve both genetic and environmental impacts, aswell as paternal age.Where genetic factors are involved, there is a concern that the widespread use of assisted conception technologies will serve to enhance the retention of poor fertility genes in the population such that the more we use assisted reproductive technologies in one generation the more we shall need them in the next. These observations may have important implications for the health and well-being of children and for the provision of reproductive healthcare services for future generations. © 2013 Faculty of 1000 Ltd.
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2013 |
De Iuliis GN, Newey RJ, King BV, Aitken RJ, 'Correction: Mobile phone radiation induces reactive oxygen species production and DNA damage in human spermatozoa in vitro (PLoS ONE)', PLoS ONE, 8 (2013)
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2013 |
Lord T, Nixon B, Jones KT, Aitken RJ, 'Melatonin Prevents Postovulatory Oocyte Aging in the Mouse and Extends the Window for Optimal Fertilization In Vitro', Biology of Reproduction, 88 1-9 (2013) [C1]
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Nova |
2013 |
Smith TB, Dun MD, Smith ND, Curry BJ, Connaughton HS, Aitken RJ, 'The presence of a truncated base excision repair pathway in human spermatozoa that is mediated by OGG1', JOURNAL OF CELL SCIENCE, 126 1488-1497 (2013) [C1]
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Nova |
2013 |
Smith R, Maiti K, Aitken RJ, 'Unexplained antepartum stillbirth: A consequence of placental aging?', PLACENTA, 34 310-313 (2013) [C3]
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Nova |
2013 |
Aitken RJ, Baker MA, 'Oxidative stress, spermatozoa and leukocytic infiltration: Relationships forged by the opposing forces of microbial invasion and the search for perfection', Journal of Reproductive Immunology, 100 11-19 (2013) [C1]
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Nova |
2013 |
Aitken RJ, Baker MA, 'Causes and consequences of apoptosis in spermatozoa; contributions to infertility and impacts on development', International Journal of Developmental Biology, 57 265-272 (2013) [C1]
During early development, apoptosis plays a major role in the ontogeny of the germ line as a means of regulating the germ cell:Sertoli cell ratio. In the adult, apoptosis fulfils ... [more]
During early development, apoptosis plays a major role in the ontogeny of the germ line as a means of regulating the germ cell:Sertoli cell ratio. In the adult, apoptosis fulfils another function in removing damaged germ cells from the seminiferous epithelium in response to a wide range of physiological and environmental triggers. These include various forms of electromagnetic radiation, chemotherapeutic agents and commonly encountered toxicants such as phthalate es-ters, bisphenol A and cadmium. This form of apoptosis can lead to spermatogenic arrest and is predominantly mediated by the Fas/FasL system. In addition, senescent mature spermatozoa can undergo a truncated form of apoptosis in order to ensure their efficient phagocytosis within the male and female reproductive tracts. This apoptotic cascade appears to be triggered by oxidative stress and lipid peroxidation, which leads to activation of mitochondrial reactive oxygen species (ROS) generation in a self-perpetuating redox cycle. The electrophilic aldehydes generated as a result of lipid peroxidation also lead to a rapid loss of sperm motility followed some hours later by caspase activation and phosphatidylserine exposure on the sperm surface. The nuclear DNA suffers oxidative damage during this process but there is no immediate DNA cleavage by endonucleases as there is in somatic cells. The reasons for this deviation from the normal pattern of apoptosis involve the unusual physical architecture of spermatozoa and the limited capacity these cells possess for base-excision repair. These findings have practical implications for the approaches that might be used to detect and prevent DNA damage in spermatozoa. © 2013 UBC Press.
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Nova |
2013 |
Barzideh J, Scott RJ, Aitken RJ, 'Analysis of the global methylation status of human spermatozoa and its association with the tendency of these cells to enter apoptosis', ANDROLOGIA, 45 424-429 (2013) [C1]
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Nova |
2013 |
Smith TB, Baker MA, Connaughton HS, Habenicht U, Aitken RJ, 'Functional deletion of Txndc2 and Txndc3 increases the susceptibility of spermatozoa to age-related oxidative stress', Free Radical Biology and Medicine, 65 872-881 (2013) [C1]
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Nova |
2013 |
Aitken RJ, 'Falling sperm counts twenty years on: where are we now?', ASIAN JOURNAL OF ANDROLOGY, 15 204-207 (2013) [C3]
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2013 |
Redgrove KA, Anderson AL, McLaughlin EA, O'Bryan MK, Aitken RJ, Nixon B, 'Investigation of the mechanisms by which the molecular chaperone HSPA2 regulates the expression of sperm surface receptors involved in human spermoocyte recognition', MOLECULAR HUMAN REPRODUCTION, 19 120-135 (2013) [C1]
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Nova |
2013 |
Aitken RJ, Bronson R, Smith TB, De Iuliis GN, 'The source and significance of DNA damage in human spermatozoa; a commentary on diagnostic strategies and straw man fallacies', Molecular Human Reproduction, 19 475-485 (2013) [C1]
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Nova |
2013 |
Aitken RJ, Nixon B, 'Sperm capacitation: a distant landscape glimpsed but unexplored', Molecular Human Reproduction, 19 785-793 (2013) [C1]
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Nova |
2013 |
Smith TB, De Iuliis GN, Lord T, Aitken RJ, 'The senescence-accelerated mouse prone 8 as a model for oxidative stress and impaired DNA repair in the male germ line', Reproduction, 146 253-262 (2013) [C1]
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Nova |
2013 |
Lord T, Aitken RJ, 'Oxidative stress and ageing of the post-ovulatory oocyte', Reproduction, 146 217-227 (2013) [C1]
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Nova |
2013 |
Lewis SEM, Aitken RJ, Conner SJ, De Iuliis G, Evenson DP, Henkel R, et al., 'The impact of sperm DNA damage in assisted conception and beyond: Recent advances in diagnosis and treatment', Reproductive BioMedicine Online, 27 325-337 (2013) [C1]
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Nova |
2013 |
Aitken RJ, 'Can spermatozoa respond to changes in their redox status with the selective activation of gene transcription?', Antioxidants and Redox Signaling, 18 184-185 (2013) [C3]
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Nova |
2013 |
Baker MA, Weinberg AS, Hetherington L, Velkov T, Aitken RJ, 'Post-ejaculatory changes in the metabolic status of rat spermatozoa as measured by GC-MS', Metabolomics, 9 708-721 (2013) [C1]
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Nova |
2013 |
Baker MA, Naumovski N, Hetherington L, Weinberg A, Velkov T, Aitken RJ, 'Head and flagella subcompartmental proteomic analysis of human spermatozoa', Proteomics, 13 61-74 (2013) [C1]
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Nova |
2013 |
Jrad-Lamine A, Henry-Berger J, Damon-Soubeyrand C, Saez F, Kocer A, Janny L, et al., 'Indoleamine 2,3-Dioxygenase 1 (Ido1) Is Involved in the Control of Mouse Caput Epididymis Immune Environment', PLOS ONE, 8 (2013) [C1]
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Nova |
2013 |
Aitken RJ, Smith TB, Lord T, Kuczera L, Koppers AJ, Naumovski N, et al., 'On methods for the detection of reactive oxygen species generation by human spermatozoa: analysis of the cellular responses to catechol oestrogen, lipid aldehyde, menadione and arachidonic acid', Andrology, 1 192-205 (2013) [C1]
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Nova |
2012 |
Clark K, Van Tongeren M, Christensen FM, Brouwer D, Nowack B, Gottschalk F, et al., 'Limitations and information needs for engineered nanomaterial-Specific exposure estimation and scenarios: Recommendations for improved reporting practices', Journal of Nanoparticle Research, 14 (2012)
The aim of this paper is to describe the process and challenges in building exposure scenarios for engineered nanomaterials (ENM), using an exposure scenario format similar to tha... [more]
The aim of this paper is to describe the process and challenges in building exposure scenarios for engineered nanomaterials (ENM), using an exposure scenario format similar to that used for the European Chemicals regulation (REACH). Over 60 exposure scenarios were developed based on information from publicly available sources (literature, books, and reports), publicly available exposure estimation models, occupational sampling campaign data from partnering institutions, and industrial partners regarding their own facilities. The primary focus was on carbon-based nanomaterials, nano-silver (nano-Ag) and nano-titanium dioxide (nano-TiO2), and included occupational and consumer uses of these materials with consideration of the associated environmental release. The process of building exposure scenarios illustrated the availability and limitations of existing information and exposure assessment tools for characterizing exposure to ENM, particularly as it relates to risk assessment. This article describes the gaps in the information reviewed, recommends future areas of ENM exposure research, and proposes types of information that should, at a minimum, be included when reporting the results of such research, so that the information is useful in a wider context. © Springer Science+Business Media B.V. 2012.
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2012 |
Aitken RJ, Gibb Z, Mitchell LA, Lambourne SR, Connaughton HS, De Iuliis GN, 'Sperm motility is lost in vitro as a consequence of mitochondrial free radical production and the generation of electrophilic aldehydes but can be significantly rescued by the presence of nucleophilic thiols', Biology of Reproduction, 87 1-11 (2012) [C1]
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Nova |
2012 |
Aitken RJ, Whiting SJ, De Iuliis GN, McClymont SL, Mitchell LA, Baker MA, 'Electrophilic aldehydes generated by sperm metabolism activate mitochondrial reactive oxygen species generation and apoptosis by targeting succinate dehydrogenase', Journal of Biological Chemistry, 287 33048-33060 (2012) [C1]
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Nova |
2012 |
Reid AT, Lord T, Stanger SJ, Roman SD, McCluskey A, Robinson PJ, et al., 'Dynamin regulates specific membrane fusion events necessary for acrosomal exocytosis in mouse spermatozoa', Journal of Biological Chemistry, 287 37659-37672 (2012) [C1]
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Nova |
2012 |
Dun MD, Anderson AL, Bromfield EG, Asquith KL, Emmett BJ, McLaughlin EA, et al., 'Investigation of the expression and functional significance of the novel mouse sperm protein, a disintegrin and metalloprotease with thrombospondin type 1 motifs number 10 (ADAMTS10)', International Journal of Andrology, 35 572-589 (2012) [C1]
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Nova |
2012 |
Jones KT, Robertson SA, Aitken RJ, 'Reactive oxygen species and sperm function-in sickness and in health', Journal of Andrology, 33 1096-1106 (2012) [C1]
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Nova |
2012 |
Aitken RJ, De Iuliis GN, Gibb Z, Baker MA, 'The Simmet lecture: New horizons on an old landscape - oxidative stress, DNA damage and apoptosis in the male germ line', Reproduction in Domestic Animals, 47 7-14 (2012) [C2]
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2012 |
Verma S, Aitken R, 'Somatic hypermutation leads to diversification of the heavy chain immunoglobulin repertoire in cattle', VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY, 145 14-22 (2012)
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2012 |
Dun MD, Aitken RJ, Nixon B, 'The role of molecular chaperones in spermatogenesis and the post-testicular maturation of mammalian spermatozoa', Human Reproduction Update, 18 420-435 (2012) [C1]
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Nova |
2012 |
Baker MA, Hetherington L, Weinberg AS, Naumovski N, Velkov T, Pelzing M, et al., 'Analysis of phosphopeptide changes as spermatozoa acquire functional competence in the epididymis demonstrates changes in the post-translational modification of Izumo1', Journal of Proteome Research, 11 5252-5264 (2012) [C1]
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Nova |
2012 |
Lo JCY, Jamsai D, O'Connor AE, Borg C, Clark BJ, Whisstock JC, et al., 'RAB-Like 2 has an essential role in male fertility, sperm intra-flagellar transport, and tail assembly', PLOS Genetics, 8 (2012) [C1]
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Nova |
2012 |
Redgrove KA, Nixon B, Baker MA, Hetherington L, Baker G, Liu D-Y, Aitken RJ, 'The molecular chaperone HSPA2 plays a key role in regulating the expression of sperm surface receptors that mediate sperm-egg recognition', Plos One, 7 1-16 (2012) [C1]
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Nova |
2012 |
Baker MA, Nixon B, Naumovski N, Aitken RJ, 'Proteomic insights into the maturation and capacitation of mammalian spermatozoa', Systems Biology in Reproductive Medicine, 58 211-217 (2012) [C1]
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Nova |
2011 |
Aitken RJ, Curry BJ, 'Redox regulation of human sperm function: From the physiological control of sperm capacitation to the etiology of infertility and DNA damage in the germ line', Antioxidants & Redox Signaling, 14 367-381 (2011) [C1]
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Nova |
2011 |
Baker MA, Smith ND, Hetherington L, Pelzing M, Condina MR, Aitken RJ, 'Use of titanium dioxide to find phosphopeptide and total protein changes during epididymal sperm maturation', Journal of Proteome Research, 10 1004-1017 (2011) [C1]
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Nova |
2011 |
Osmond-McLeod MJ, Poland CA, Murphy F, Waddington L, Morris H, Hawkins SC, et al., 'Durability and inflammogenic impact of carbon nanotubes compared with asbestos fibres', Particle and Fibre Toxicology, 8 (2011)
Background: It has been suggested that carbon nanotubes might conform to the fibre pathogenicity paradigm that explains the toxicities of asbestos and other fibres on a continuum ... [more]
Background: It has been suggested that carbon nanotubes might conform to the fibre pathogenicity paradigm that explains the toxicities of asbestos and other fibres on a continuum based on length, aspect ratio and biopersistence. Some types of carbon nanotubes satisfy the first two aspects of the fibre paradigm but only recently has their biopersistence begun to be investigated. Biopersistence is complex and requires in vivo testing and analysis. However durability, the chemical mimicking of the process of fibre dissolution using in vitro treatment, is closely related to biopersistence and more readily determined. Here, we describe an experimental process to determine the durability of four types of carbon nanotubes in simulated biological fluid (Gambles solution), and their subsequent pathogenicity in vivo using a mouse model sensitive to inflammogenic effects of fibres. The in vitro and in vivo results were compared with well-characterised glass wool and asbestos fibre controls.Results: After incubation for up to 24 weeks in Gambles solution, our control fibres were recovered at percentages consistent with their known in vitro durabilities and/or in vivo persistence, and three out of the four types of carbon nanotubes tested (single-walled (CNTSW) and multi-walled (CNTTANG2, CNTSPIN)) showed no, or minimal, loss of mass or change in fibre length or morphology when examined by electron microscopy. However, the fourth type [multi-walled (CNTLONG1)] lost 30% of its original mass within the first three weeks of incubation, after which there was no further loss. Electron microscopy of CNTLONG1 samples incubated for 10 weeks confirmed that the proportion of long fibres had decreased compared to samples briefly exposed to the Gambles solution. This loss of mass and fibre shortening was accompanied by a loss of pathogenicity when injected into the peritoneal cavities of C57Bl/6 mice compared to fibres incubated briefly. CNTSW did not elicit an inflammogenic effect in the peritoneal cavity assay used here.Conclusions: These results support the view that carbon nanotubes are generally durable but may be subject to bio-modification in a sample-specific manner. They also suggest that pristine carbon nanotubes, either individually or in rope-like aggregates of sufficient length and aspect ratio, can induce asbestos-like responses in mice, but that the effect may be mitigated for certain types that are less durable in biological systems. Results indicate that durable carbon nanotubes that are either short or form tightly bundled aggregates with no isolated long fibres are less inflammogenic in fibre-specific assays. © 2011 Osmond-McLeod et al; licensee BioMed Central Ltd.
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2011 |
Aitken RJ, 'The capacitation-apoptosis highway: Oxysterols and mammalian sperm function', Biology of Reproduction, 85 9-12 (2011) [C3]
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2011 |
Redgrove KA, Anderson AL, Dun MD, McLaughlin EA, O'Bryan MK, Aitken RJ, Nixon B, 'Involvement of multimeric protein complexes in mediating the capacitation-dependent binding of human spermatozoa to homologous zonae pellucidae', Developmental Biology, 356 460-474 (2011) [C1]
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Nova |
2011 |
Jrad-Lamine A, Henry-Berger J, Gourbeyre P, Damon-Soubeyrand C, Lenoir A, Combaret L, et al., 'Deficient tryptophan catabolism along the kynurenine pathway reveals that the epididymis is in a unique tolerogenic state', Journal of Biological Chemistry, 286 8030-8042 (2011) [C1]
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Nova |
2011 |
Dun MD, Smith ND, Baker MA, Lin M, Aitken RJ, Nixon B, 'The chaperonin containing TCP1 complex (CCT/TRiC) is involved in mediating sperm-oocyte interaction', Journal of Biological Chemistry, 286 36875-36887 (2011) [C1]
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Nova |
2011 |
Nixon B, Mitchell LA, Anderson AL, McLaughlin EA, O'Bryan MK, Aitken RJ, 'Proteomic and functional analysis of human sperm detergent resistant membranes', Journal of Cellular Physiology, 226 2651-2665 (2011) [C1]
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Nova |
2011 |
Ainsworth CJ, Nixon B, Aitken RJ, 'The electrophoretic separation of spermatozoa: An analysis of genotype, surface carbohydrate composition and potential for capacitation', International Journal of Andrology, 34 e422-e434 (2011) [C1]
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Nova |
2011 |
Aitken RJ, Carey AJ, Beagley KW, 'Dual purpose contraceptives: targeting fertility and sexually transmitted disease', Journal of Reproductive Immunology, 88 228-232 (2011) [C1]
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Nova |
2011 |
Koppers AJ, Mitchell LA, Wang JP, Lin M, Aitken RJ, 'Phosphoinositide 3-kinase signalling pathway involvement in a truncated apoptotic cascade associated with motility loss and oxidative DNA damage in human spermatozoa', Biochemical Journal, 436 687-698 (2011) [C1]
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Nova |
2011 |
Aitken RJ, Mitchell L, De Iuliis GN, 'Reply: M540 bodies interfere with TUNEL analyses in human semen samples', HUMAN REPRODUCTION, 26 729-730 (2011) [C3]
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2011 |
Aitken RJ, Hanson AR, Kuczera LH, 'Electrophoretic sperm isolation: Optimization of electrophoresis conditions and impact on oxidative stress', Human Reproduction, 26 1955-1964 (2011) [C1]
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Nova |
2011 |
Gharagozloo P, Aitken RJ, 'The role of sperm oxidative stress in male infertility and the significance of oral antioxidant therapy', Human Reproduction, 26 1628-1640 (2011) [C1]
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Nova |
2011 |
McLaughlin EA, Aitken RJ, 'Is there a role for immunocontraception?', Molecular and Cellular Endocrinology, 335 78-88 (2011) [C1]
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Nova |
2011 |
Aitken RJ, Henkel R, 'Special Edition of the Asian Journal of Andrology on The Cell Biology and Genetics of Spermatozoa', Asian Journal of Andrology, 13 3-5 (2011) [C6] |
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Nova |
2011 |
Aitken RJ, Koppers AJ, 'Apoptosis and DNA damage in human spermatozoa', Asian Journal of Andrology, 13 36-42 (2011) [C1]
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Nova |
2011 |
Aitken RJ, Henkel RR, 'Sperm cell biology: current perspectives and future prospects', Asian Journal of Andrology, 13 3-5 (2011) [C3]
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2011 |
Gattellari M, Goumas C, Aitken RJ, Worthington JM, 'Outcomes for patients with ischaemic stroke and atrial fibrillation: The PRISM Study (A Program of Research Informing Stroke Management)', Cerebrovascular Diseases, 32 370-382 (2011) [C1]
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Nova |
2011 |
Aitken RJ, Baker MA, 'Cell biology of human spermatozoa: Lessons from the Proteome', Australian Biochemist, 42 12-15 (2011) [C3]
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2011 |
Aitken RJ, Findlay JK, Hutt KJ, Kerr JB, 'Apoptosis in the germ line', Reproduction, 141 139-150 (2011) [C1]
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Nova |
2011 |
Aitken RJ, Henkel R, 'Special Edition of the Asian Journal of Andrology on The Cell Biology and Genetics of Spermatozoa', Asian Journal of Andrology, 13 3-5 (2011) [C6] |
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Nova |
2010 |
Baker MA, Smith ND, Hetherington L, Taubman K, Graham ME, Robinson PJ, Aitken RJ, 'Label-free quantitation of phosphopeptide changes during rat sperm capacitation', Journal of Proteome Research, 9 718-729 (2010) [C1]
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Nova |
2010 |
Baker MA, Reeves GM, Hetherington L, Aitken RJ, 'Analysis of proteomic changes associated with sperm capacitation through the combined use of IPG-strip pre-fractionation followed by RP chromatography LC-MS/MS analysis', Proteomics, 10 482-495 (2010) [C1]
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Nova |
2010 |
Shokri S, Aitken RJ, Abdolvahhabi M, Abolhasani F, Ghasemi FM, Kashani I, et al., 'Exercise and supraphysiological dose of nandrolone deconoate increase apoptosis in spermatogenic cells', Basic & Clinical Pharmacology & Toxicology, 106 324-330 (2010) [C1]
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Nova |
2010 |
Denney-Wilson E, Cowell CT, Okely AD, Hardy LL, Aitken R, Dobbins T, 'Associations between insulin and glucose concentrations and anthropometric measures of fat mass in Australian adolescents', BMC Pediatrics, 10 (2010)
Background: One of the most serious, yet common co-morbidities of obesity is insulin resistance, which if untreated may progress to type 2 diabetes. This paper describes the insul... [more]
Background: One of the most serious, yet common co-morbidities of obesity is insulin resistance, which if untreated may progress to type 2 diabetes. This paper describes the insulin and glucose concentration distributions, the prevalence of elevated insulin, the associations between insulin and body mass index (BMI), waist circumference, waist-to-height ratio (WHtR) and fat mass index in a representative sample of Australian adolescents.Methods: Cross-sectional population-based study of adolescent boys and girls (N = 496, mean age 15.3 years) attending schools in metropolitan Sydney, Australia. Fasting venous blood collected and analysed for insulin and glucose concentrations. Height, weight, waist circumference measured, BMI and waist-to-height ratio calculated. Pubertal status self-reported.Results: Glucose concentrations were normally distributed and were not associated with adiposity. Insulin concentrations were distributed logarithmically, were higher among girls than boys overall and within the same ranges of BMI and waist circumference, but were lower among girls than boys within the same ranges of fat mass adjusted for height. The prevalence of elevated insulin concentration (defined as > 100 pmol/L) was 15.9% and 17.1% among boys and girls, respectively. Correlations between insulin concentration and BMI, waist circumference, WHtR and fat mass adjusted for height were 0.53, 0.49, 0.51 and 0.55, among boys, respectively, and 0.35, 0.40, 0.42 and 0.34, among girls, respectively.Conclusions: Elevated insulin is highly correlated with adiposity in adolescents. BMI and WHtR are simple measures that can be used to identify young people who should be screened for insulin resistance and other co-morbidities. © 2010 Denney-Wilson et al; licensee BioMed Central Ltd.
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2010 |
Pujianto DA, Curry BJ, Aitken RJ, 'Prolactin exerts a prosurvival effect on human spermatozoa via mechanisms that involve the stimulation of Akt phosphorylation and suppression of caspase activation and capacitation', Endocrinology, 151 1269-1279 (2010) [C1]
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Nova |
2010 |
Naraghi MA, Abolhasani F, Kashani I, Anarkooli IJ, Hemadi M, Azami A, et al., 'The effects of swimming exercise and supraphysiological doses of nandrolone decanoate on the testis in adult male rats: A transmission electron microscope study', Folia Morphologica, 69 138-146 (2010) [C1]
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Nova |
2010 |
Verma S, Goldammer T, Aitken R, 'Cloning and expression of activation induced cytidine deaminase from Bos taurus', VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY, 134 151-159 (2010)
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2010 |
Barratt CLR, Aitken RJ, Bjorndahl L, Carrell DT, De Boer P, Kvist U, et al., 'Sperm DNA: Organization, protection and vulnerability: From basic science to clinical applications - A position report', Human Reproduction, 25 824-838 (2010) [C1]
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Nova |
2010 |
Aitken RJ, De Iuliis GN, Finnie JM, Hedges A, McLachlan RI, 'Analysis of the relationships between oxidative stress, DNA damage and sperm vitality in a patient population: Development of diagnostic criteria', Human Reproduction, 25 2415-2426 (2010) [C1]
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Nova |
2010 |
Koppers AJ, Garg ML, Aitken RJ, 'Stimulation of mitochondrial reactive oxygen species production by unesterified, unsaturated fatty acids in defective human spermatozoa', Free Radical Biology and Medicine, 48 112-119 (2010) [C1]
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Nova |
2010 |
Mitchell LA, De Iuliis GN, Aitken RJ, 'The TUNEL assay consistently underestimates DNA damage in human spermatozoa and is influenced by DNA compaction and cell vitality: Development of an improved methodology', International Journal of Andrology, 34 2-13 (2010) [C1]
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2010 |
Aitken RJ, 'Whither must spermatozoa wander? The future of laboratory seminology', Asian Journal of Andrology, 12 99-103 (2010) [C3]
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2010 |
Reid AT, Redgrove KA, Aitken RJ, Nixon B, 'Cellular mechanisms regulating sperm-zona pellucida interaction', Asian Journal of Andrology, 13 88-96 (2010) [C1]
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2010 |
Nixon B, Bielanowicz AJ, Anderson AL, Walsh AK, Hall TE, McCloghry AK, Aitken RJ, 'Elucidation of the signaling pathways that underpin capacitation-associated surface phosphotyrosine expression in mouse spermatozoa', Journal of Cellular Physiology, 224 71-83 (2010) [C1]
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Nova |
2010 |
Aitken RJ, De Iuliis GN, 'On the possible origins of DNA damage in human spermatozoa', Molecular Human Reproduction, 16 3-13 (2010) [C1]
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Nova |
2009 |
Baker MA, Aitken RJ, 'Proteomic insights into spermatozoa: Critiques, comments and concerns', Expert Review of Proteomics, 6 691-705 (2009) [C1]
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Nova |
2009 |
Ewen KA, Baker MA, Wilhelm D, Aitken RJ, Koopman P, 'Global survey of protein expression during gonadal sex determination in mice', Molecular & Cellular Proteomics, 8 2624-2641 (2009) [C1]
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Nova |
2009 |
De Iuliis GN, Newey RJ, King BV, Aitken RJ, 'Mobile phone radiation induces reactive oxygen species production and DNA damage in human spermatozoa in vitro', PLoS ONE, 4 e6446 (2009) [C1]
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Nova |
2009 |
Bennetts LE, De Iuliis GN, Nixon B, Kime M, Zelski K, McVicar CM, et al., 'Impact of Estrogenic Compounds on DNA Integrity in Human Spermatozoa: Evidence for Cross-Linking and Redox Cycling Activities', JOURNAL OF UROLOGY, 181 914-915 (2009) [C1]
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2009 |
Booth ML, Dobbins T, Aitken R, Denney-Wilson E, Hardy LL, Okely AD, et al., 'Costs of managing conditions associated with obesity among Australian teenagers', Journal of Paediatrics and Child Health, 45 448-456 (2009)
Aim: To determine the health-care charges associated with monitoring and managing, over 1 year, the cases of elevated insulin concentration, elevated alanine aminotransferase conc... [more]
Aim: To determine the health-care charges associated with monitoring and managing, over 1 year, the cases of elevated insulin concentration, elevated alanine aminotransferase concentration and dyslipidaemia due to overweight or obesity among 15-19-year-old Australian males and females. Methods: Fasting blood samples (n = 500) were collected in 2004 from a representative population sample of adolescents (n = 496; mean age 15.3 years) attending schools in Sydney, Australia. Full service charges and Medicare expenditures for specialist medical and dietary consultations, pathology tests and radiological investigations, over 1 year, under efficient and inefficient health-care delivery models, including and excluding participants in the healthy body mass index (BMI) category. Results: Under an inefficient delivery model and including all participants with elevated risk factors, the Medicare expenditure was $A305.1 million per annum (M pa). Exclusion of participants in the healthy BMI category resulted in an annual Medicare expenditure of $A170.0M pa. Under an efficient delivery model and including all participants with elevated risk factors, the Medicare expenditure was $A295.5M pa. Exclusion of participants in the healthy BMI category reduced annual Medicare expenditure to $A164.8M pa. Medicare expenditure for 15-19-year-olds would increase by 48% if only cases among overweight and obese adolescents were treated and by 85% if all cases were identified and treated. Conclusions: Short-term management of the health consequences of overweight and obesity among adolescents will increase Medicare expenditure on this group by at least 48%. Failure to treat will delay, but compound, health-care expenditure. © 2009 Paediatrics and Child Health Division (Royal Australasian College of Physicians).
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2009 |
Aitken R, Borm P, Donaldson K, Ichihara G, Loft S, Marano F, et al., 'Editorial: Nanoparticles one word: A multiplicity of different hazards Editorial Editorial December2009 34', Nanotoxicology, 3 263-264 (2009)
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2009 |
De Iuliis GN, Thomson LK, Mitchell LA, Read JM, Koppers AJ, Hedges A, et al., 'DNA damage in human spermatozoa is highly correlated with the efficiency of chromatin remodeling and the formation of 8-hydroxy-2'-deoxyguanosine, a marker of oxidative stress', Biology of Reproduction, 81 517-524 (2009) [C1]
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Nova |
2009 |
Baker MA, Hetherington L, Curry BJ, Aitken RJ, 'Phosphorylation and consequent stimulation of the tyrosine kinase c-Abl by PKA in mouse spermatozoa: Its implications during capacitation', Developmental Biology, 333 57-66 (2009) [C1]
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Nova |
2009 |
Nixon B, Bielanowicz AJ, McLaughlin EA, Tanphaichitr N, Ensslin MA, Aitken RJ, 'Composition and significance of detergent resistant membranes in mouse spermatozoa', Journal of Cellular Physiology, 218 122-134 (2009) [C1]
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Nova |
2009 |
Aitken RJ, 'Gpx5 protects the family jewels', Journal of Clinical Investigation, 119 1849-1851 (2009) [C1]
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Nova |
2009 |
Hughes LM, Griffith R, Carey A, Butler TA, Donne SW, Beagley KW, Aitken RJ, 'The spermostatic and microbicidal actions of quinones and maleimides: Toward a dual-purpose contraceptive agent', Molecular Pharmacology, 76 113-124 (2009) [C1]
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Nova |
2009 |
Aitken RJ, De Iuliis GN, McLachlan RI, 'Biological and clinical significance of DNA damage in the male germ line', International Journal of Andrology, 32 46-56 (2009) [C1]
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Nova |
2009 |
Nixon B, Aitken RJ, 'The biological significance of detergent-resistant membranes in spermatozoa', Journal of Reproductive Immunology, 83 8-13 (2009) [C1]
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Nova |
2009 |
Thomson LK, Fleming SD, Aitken RJ, De Iuliis GN, Zieschang JA, Clark AM, 'Cryopreservation-induced human sperm DNA damage is predominantly mediated by oxidative stress rather than apoptosis', Human Reproduction, 24 2061-2070 (2009) [C1]
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Nova |
2008 |
Mitchell LA, Nixon B, Baker MA, Aitken RJ, 'Investigation of the role of SRC in capacitation-associated tyrosine phosphorylation of human spermatozoa', Molecular Human Reproduction, 14 235-243 (2008) [C1]
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Nova |
2008 |
Baker MA, Hetherington L, Reeves GM, Muller J, Aitken RJ, 'The rat sperm proteome characterized via IPG strip prefractionation and LC-MS/MS identification', Proteomics, 8 2312-2321 (2008) [C1]
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Nova |
2008 |
Baker MA, Hetherington L, Reeves GM, Aitken RJ, 'The mouse sperm proteome characterized via IPG strip prefractionation and LC-MS/MS identification', Proteomics, 8 1720-1730 (2008) [C1]
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Nova |
2008 |
Aitken RJ, 'Just how safe is assisted reproductive technology for treating, male factor infertility?', Expert Review of Obstetrics and Gynecology, 3 267-271 (2008) [C1]
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Nova |
2008 |
Aitken RJ, Roman SD, 'Antioxidant systems and oxidative stress in the testes (Reprinted from Molecular Mechanisms in Spermatogenesis, 2007)', OXIDATIVE MEDICINE AND CELLULAR LONGEVITY, 1 15-24 (2008) [C3]
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2008 |
George J, Denney-Wilson E, Okely AD, Hardy LL, Aitken R, Booth ML, 'The population distributions, upper normal limits and correlations between liver tests among Australian adolescents', Journal of Paediatrics and Child Health, 44 579-585 (2008)
Aim: Relatively little is known about the results of liver tests among adolescents. The purpose of this study was to describe the distributions of liver tests, to identify the upp... [more]
Aim: Relatively little is known about the results of liver tests among adolescents. The purpose of this study was to describe the distributions of liver tests, to identify the upper normal limits (UNLs) and to describe the correlations among liver tests. Methods: Overnight fasting blood samples were collected from a representative population sample of 500 Grade 10 students (15 years old) attending schools in Sydney, Australia. Weight, height and waist girth were measured. UNLs were calculated for each enzyme as the 95th percentiles of the healthy body mass index category, after excluding those with high blood pressure, elevated low-density lipoprotein cholesterol (=3.4 mmol/L) or triglyceride concentration (=2.25 mmol/L) (n = 246). The distributions of alanine aminotransferase (ALT), ¿-glutamyltransferase (GGT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP) were described, UNLs were identified and the correlations between enzyme concentrations were described. Results: The UNLs for ALT were 32 and 20 U/L; for GGT, 24 and 19 U/L; for AST, 33 and 26 U/L; and for ALP, 385 and 183 U/L, for boys and girls, respectively. ALT concentration was strongly correlated with GGT and AST (r = 0.5-0.6). Neither ALT nor GGT concentrations were correlated with ALP concentration, but AST concentration was moderately correlated with ALP concentration. Conclusions: These data are valuable in defining the distributions of liver tests, normal liver test ranges and relationships between liver tests among adolescents. © 2008 The Authors.
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2008 |
Booth ML, George J, Denney-Wilson E, Okely AD, Hardy LL, Aitken R, Dobbins T, 'The population prevalence of adverse concentrations and associations with adiposity of liver tests among Australian adolescents', Journal of Paediatrics and Child Health, 44 686-691 (2008)
Aim: Paediatric overweight has a high incidence and has serious consequences for health, including non-alcoholic fatty liver disease (NAFLD). However, very little is known about N... [more]
Aim: Paediatric overweight has a high incidence and has serious consequences for health, including non-alcoholic fatty liver disease (NAFLD). However, very little is known about NAFLD among young people, particularly from a population perspective. This paper reports the prevalence of elevated concentrations of four liver enzymes and their associations with adiposity in a representative population sample of Australian adolescents. Methods: Overnight fasting blood samples were collected from a representative population sample of 500 Grade 10 students (15-years-old) attending schools in Sydney, Australia, between February and May, 2004. Weight, height and waist girth were measured. The prevalence of adverse concentrations of the enzymes alanine aminotransferase (ALT), gamma-glutamyltransferase (GGT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP) were described for all boys and girls and by body mass index (BMI) categories. The nature of the associations between the enzyme concentrations and indices of adiposity were characterised, and regression equations for estimating enzyme concentrations from BMI were prepared. Results: The prevalence of adverse concentrations of ALT (most likely as a result of NAFLD) and GGT were approximately 10%, while the prevalence of adverse concentrations of AST and ALP were approximately 7% and 5% respectively. The prevalence of adverse concentrations of ALT, GGT and AST increased across BMI categories, while the prevalence of adverse concentrations of ALP declined across BMI categories. Conclusions: The high prevalence of NAFLD in a population sample of healthy Australian adolescents represents a significant burden of disease. © 2008 The Authors.
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2008 |
Grigor KM, Jensen K, Grandjean P, Jensen TK, Bittner G, Sobotka T, et al., 'Adverse trends in male reproductive health: we may have reached a crucial 'tipping point' - Panel discussion', INTERNATIONAL JOURNAL OF ANDROLOGY, 31 89-92 (2008) |
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2008 |
Aitken RJ, Hauser R, Jensen TK, Hauser R, Skakkebaek NE, Hauser R, et al., 'Urinary phthalate metabolites and semen quality: a review of a potential biomarker of susceptibility - Panel discussion', INTERNATIONAL JOURNAL OF ANDROLOGY, 31 116-117 (2008)
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2008 |
Golchin M, Aitken R, 'Isolation by phage display of recombinant antibodies able to block adherence of Escherichia coli mediated by the K99 colonisation factor', VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY, 121 321-331 (2008)
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2008 |
Aitken R, King L, Bauman A, 'A comparison of Australian families' expenditure on active and screen-based recreation using the ABS Household Expenditure Survey 2003/04', AUSTRALIAN AND NEW ZEALAND JOURNAL OF PUBLIC HEALTH, 32 238-245 (2008)
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2008 |
Walsh AK, Whelan D, Bielanowicz AJ, Skinner BL, Aitken RJ, O'Bryan MK, Nixon B, 'Identification of the molecular chaperone, heat shock protein 1 (chaperonin 10), in the reproductive tract and in capacitating spermatozoa in the male mouse', Biology of Reproduction, 78 983-993 (2008) [C1]
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Nova |
2008 |
Aitken RJ, Hughes LM, Griffith R, Baker MA, 'Bridging the gap between male and female fertility control; contraception-on-demand', Contraception, 78 S28-S35 (2008) [C1]
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Nova |
2008 |
McLachlan RI, Aitken RJ, Cram D, Krausz C, O'Bryan M, 'Need for standardization and confirmation of STS deletions on the Y chromosome', Fertility and Sterility, 90 463-464 (2008) [C3]
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Nova |
2008 |
Koppers AJ, De Iuliis GN, Read JM, McLaughlin EA, Aitken RJ, 'Significance of mitochondrial reactive oxygen species in the generation of oxidative stress in spermatozoa', Journal of Clinical Endocrinology & Metabolism, 93 3199-3207 (2008) [C1]
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Nova |
2008 |
Aitken RJ, Baker MA, Doncel GF, Matzuk MM, Mauck CK, Harper MJK, 'As the world grows: Contraception in the 21st century', Journal of Clinical Investigation, 118 1330-1343 (2008) [C1]
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Nova |
2008 |
Aitken RJ, Baker MA, 'The role of proteomics in understanding sperm cell biology', International Journal of Andrology, 31 295-302 (2008) [C1]
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2008 |
Fleming SD, Ilad RS, Griffin AMG, Wu Y, Ong KJ, Smith HC, Aitken RJ, 'Prospective controlled trial of an electrophoretic method of sperm preparation for assisted reproduction: Comparison with density gradient centrifugation', Human Reproduction, 23 2646-2651 (2008) [C1]
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Nova |
2007 |
Hughes LM, Griffith R, Aitken RJ, 'The search for a topical dual action spermicide/microbicide', Current Medicinal Chemistry, 14 775-786 (2007) [C1]
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Nova |
2007 |
Aitken RJ, Nixon B, Lin M, Koppers AJ, Lee YH, Baker MA, 'Proteomic changes in mammalian spermatozoa during epididymal maturation', Asian Journal of Andrology, 9 554-564 (2007) [C1]
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2007 |
Mitchell LA, Nixon B, Aitken RJ, 'Analysis of chaperone proteins associated with human spermatozoa during capacitation', Molecular Human Reproduction, 13 605-613 (2007) [C1]
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2007 |
Aitken RJ, Wingate JK, De Iuliis GN, McLaughlin EA, 'Analysis of lipid peroxidation in human spermatozoa using BODIPY C11', Molecular Human Reproduction, 13 203-211 (2007) [C1]
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2007 |
Nixon B, Aitken RJ, McLaughlin EA, 'New insights into the molecular mechanisms of sperm-egg interaction', Cellular and Molecular Life Sciences, 64 1805-1823 (2007) [C1]
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2007 |
Baker MA, Reeves GE, Hetherington L, Muller J, Baur I, Aitken RJ, 'Identification of gene products present in Triton X-100 soluble and insoluble fractions of human spermatozoa lysates using LC-MS/MS analysis', Proteomics Clinical Applications, 1 524-532 (2007) [C1]
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2007 |
Hughes L, Wingate JK, Griffith R, Aitken RJ, 'Modelling and measuring redox cycling and cytotoxicity of quinones', Drug Metabolism Letters, 1 245-253 (2007) [C2] |
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2007 |
Aitken RJ, De Iuliis GN, 'Value of DNA integrity assays for fertility evaluation', Society for Reproduction and Fertility Supplement, 65 81-92 (2007) [C1]
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2007 |
Aitken RJ, McLaughlin EA, 'Molecular mechanisms of sperm capacitation: progesterone-induced secondary calcium oscillations reflect the attainment of a capacitated state', Society of Reproduction and Fertility Supplement, 63 273-293 (2007) [C1]
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2007 |
Tran L, Aitken R, Ayres J, Donaldson K, Hurley F, 'Human effects of nanoparticle exposure', Issues in Environmental Science and Technology, 24 102-117 (2007)
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2007 |
Aitken RJ, De Iuliis GN, 'Origins and consequences of DNA damage in male germ cells', Reproductive Biomedicine Online, 14 727-733 (2007) [C1]
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2007 |
Gibbs GM, Bianco DM, Jamsai D, Herlihy A, Ristevski S, Aitken RJ, et al., 'Cysteine-rich secretory protein 2 binds to mitogen-activated protein kinase kinase kinase 11 in mouse sperm', Biology of Reproduction, 77 108-114 (2007) [C1]
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2007 |
Wathes DC, Abayasekara DRE, Aitken RJ, 'Polyunsaturated fatty acids in male and female reproduction', Biology of Reproduction, 77 190-201 (2007) [C1]
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2007 |
Ainsworth CJ, Nixon B, Jansen RPS, Aitken RJ, 'First recorded pregnancy and normal birth after ICSI using electrophoretically isolated spermatozoa', Human Reproduction, 22 197-200 (2007) [C1]
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2006 |
Baker MA, Hetherington L, Aitken RJ, 'Identification of SRC as a key PKA-stimulated tyrosine kinase involved in the capacitation-associated hyperactivation of murine spermatozoa', Journal of Cell Science, 119 3182-3192 (2006) [C1]
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Nova |
2006 |
De Iuliis GN, Wingate JK, Koppers AJ, McLaughlin EA, Aitken RJ, 'Definitive evidence for the nonmitochondrial production of superoxide anion by human spermatozoa', Journal of Clinical Endocrinology and Metabolism, 91 1968-1975 (2006) [C1]
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Nova |
2006 |
Aitken RJ, Wingate JK, De Iuliis GN, Koppers AJ, McLaughlin EA, 'Cis-unsaturated Fatty acids stimulate reactive oxygen species generation and lipid peroxidation in human spermatozoa', Journal of Clinical Endocrinology and Metabolism, 91 4154-4163 (2006) [C1]
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Nova |
2006 |
Holt JE, Roman SD, Aitken RJ, McLaughlin EA, 'Identification and characterization of a novel Mt-retrotransposon highly represented in the female mouse germline', Genomics, 87 490-499 (2006) [C1]
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2006 |
Curry BJ, Holt JE, McLaughlin EA, Aitken RJ, 'Characterization of structure and expression of the Dzip1 gene in the rat and mouse', Genomics, 87 275-285 (2006) [C1]
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2006 |
Aitken RJ, Skakkebaek NE, Roman SD, 'Male reproductive health and the environment (Editorial)', Medical Journal of Australia, 185 414-415 (2006) [C3]
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Nova |
2006 |
Holt JE, Jackson A, Roman SD, Aitken RJ, Koopman PA, McLaughlin EA, 'CXCR4/SDF1 interaction inhibits the primordial to primary follicle transition in the neonatal mouse ovary', Developmental Biology, 293 449-460 (2006) [C1]
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Nova |
2006 |
Habenicht UF, Harper M, Wang YF, de Kretser D, Aitken RJ, 'Round table discussion on "Public health: contribution of family planning towards public health"', MOLECULAR AND CELLULAR ENDOCRINOLOGY, 250 212-213 (2006)
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2006 |
Aitken RJ, Baker MA, 'Oxidative stress, sperm survival and fertility control', Molecular and Cellular Endocrinology, 250 66-69 (2006) [C1]
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2006 |
Habenicht UF, Harper M, Wang YF, De Kretser D, Aitken RJ, 'Public health: contribution of family planning towards public health (Round table discussion)', Molecular and Cellular Endocrinology, 250 212-213 (2006) [C2] |
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2006 |
Gasanov U, Koina CA, Beagley KW, Aitken RJ, Hansbro PM, 'Identification of the insulin-like growth factor II receptor as a novel receptor for binding and invasion by Listeria monocytogenes', Infection and Immunity, 74 566-577 (2006) [C1]
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2006 |
Aitken RJ, 'Sperm function tests and fertility', International Journal of Andrology, 29 69-74 (2006) [C1]
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Nova |
2006 |
Maynard AD, Aitken RJ, Butz T, Colvin V, Donaldson K, Oberdoerster G, et al., 'Safe handling of nanotechnology', NATURE, 444 267-269 (2006)
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2006 |
Leridon H, Lutz W, Aitken RJ, Lutz W, von Eyben FE, 'Fertility rates and future population trends: Will Europe's birth rate recover or continue to decline? Discussion', INTERNATIONAL JOURNAL OF ANDROLOGY, 29 32-33 (2006) |
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2006 |
Jegou B, Aitken RJ, Skakkebaek N, Eliasson R, Jorgensen N, 'Sperm function tests and fertility - Discussion', INTERNATIONAL JOURNAL OF ANDROLOGY, 29 74-75 (2006) |
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2006 |
Skakkebaek NE, Franks S, Aitken RJ, Swan S, Keizer-Schrama SD, 'Development of polycystic ovary syndrome: involvement of genetic and environmental factors - Discussion', INTERNATIONAL JOURNAL OF ANDROLOGY, 29 284-285 (2006) |
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2006 |
Nixon B, Macintyre DA, Mitchell LA, Gibbs GM, O'Bryan M, Aitken RJ, 'The identification of mouse sperm-surface-associated proteins and characterization of their ability to act as decapacitation factors', Biology of Reproduction, 74 275-287 (2006) [C1]
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Nova |
2006 |
Lin M, Lee YH, Xu W, Baker MA, Aitken RJ, 'Ontogeny of tyrosine phosphorylation-signaling pathways during spermatogenesis and epididymal maturation in the mouse', Biology of Reproduction, 75 588-597 (2006) [C1]
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Nova |
2005 |
Nixon B, Paul JW, Spiller CM, Attwell-Heap AG, Ashman LK, Aitken RJ, 'Evidence for the involvement of PECAM-1 in a receptor mediated signal-transduction pathway regulating capacitation-associated tyrosine phosphorylation in human spermatozoa', Journal of Cell Science, 118 4865-4877 (2005) [C1]
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Nova |
2005 |
Lewis SEM, Aitken RJ, 'DNA damage to spermatozoa has impacts on fertilization and pregnancy', Cell and Tissue Research, 322 33-41 (2005) [C1]
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2005 |
Nixon B, Asquith KL, Aitken RJ, 'The role of molecular chaperones in mouse sperm-egg interactions', Molecular and Cellular Endocrinology, 240 1-10 (2005) [C1]
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Nova |
2005 |
Ainsworth CJ, Nixon B, Aitken RJ, 'Development of a novel electrophoretic system for the isolation of human spermatozoa', Human Reproduction, 20 2261-2270 (2005) [C1]
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Nova |
2005 |
Baleato R, Aitken RJ, Roman SD, 'Vitamin A regulation of BMP4 expression in the male germ line', Developmental Biology, 286 78-90 (2005) [C1]
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Nova |
2005 |
Ewen KA, Wilhelm D, Aitken RJ, Koopman PA, 'Identification and characterisation of proteins influencing mammalian germ cell differentiation', MECHANISMS OF DEVELOPMENT, 122 S119-S119 (2005) |
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2005 |
Roman SD, Baleato RM, Aitken RJ, 'Interaction between BMP4 and retinoid signaling in mouse spermatogenesis', MECHANISMS OF DEVELOPMENT, 122 S126-S126 (2005) |
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2005 |
Asquith KL, Bielanowicz AJ, McLaughlin EA, Nixon B, Aitken RJ, 'Localization and significance of molecular chaperones, heat shock protein 1, and tumor rejection antigen gp96 in the male reproductive tract and during capacitation and acrosome reaction', Biology of Reproduction, 72 328-337 (2005) [C1]
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Nova |
2005 |
Bennetts LE, Aitken RJ, 'A comparative study of oxidative DNA damage in mammalian spermatozoa', Molecular Reproduction and Development, 71 77-87 (2005) [C1]
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Nova |
2005 |
Aitken RJ, Bennetts LE, Sawyer D, Wiklendt AM, King BV, 'Impact of radio frequency electromagnetic radiation on DNA integrity in the male germline', International Journal of Andrology, 28 171-179 (2005) [C1]
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Nova |
2005 |
Baker MA, Aitken RJ, 'Reactive oxygen species in spermatozoa: methods for monitoring and significance for the origins of genetic disease and infertility', Reproductive Biology and Endocrinology, 3 9 (2005) [C1]
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2005 |
Baker MA, Krutskikh A, Curry BJ, Hetherington L, Aitken RJ, 'Identification of cytochrome-b5 reductase as the enzyme responsible for NADH-dependent lucigenin chemiluminescence in human spermatozoa', Biology of Reproduction, 73 334-342 (2005) [C1]
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Nova |
2005 |
Baker MA, Witherdin RF, Hetherington L, Cunningham KA, Aitken RJ, 'Identification of post-translational modifications that occur during sperm maturation using difference in two-dimensional gel electrophoresis', Proteomics, 5 1003-1012 (2005) [C1]
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2004 |
Blackmore DG, Baillie LR, Holt JE, Dierkx LM, Aitken RJ, McLaughlin EA, 'Biosynthesis of the Canine Zona Pellucida Requires the Integrated Participation of Both Oocytes and Granulosa Cells', Biology of Reproduction, 71 661-668 (2004) [C1]
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2004 |
Baker MA, Krutskikh A, Curry BJ, McLaughlin EA, Aitken RJ, 'Identification of Cytochrome P450-Reductase as the Enzyme Responsible for NADPH-Dependent Lucigenin and Tetrazolium Salt Reduction in Rat Epididymal Sperm Preparations', Biology of Reproduction, 71 307-318 (2004) [C1]
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2004 |
Ecroyd HW, Asquith KL, Jones RC, Aitken RJ, 'The development of signal transduction pathways during epididymal maturation is calcium dependent', Developmental Biology, 268 53-63 (2004) [C1]
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Nova |
2004 |
Baker MA, Hetherington L, Ecroyd HW, Roman SD, Aitken RJ, 'Analysis of the mechanism by which calcium negatively regulates the tyrosine phoshorylation cascade associated with sperm capacitation', Journal of Cell Science, 117 211-222 (2004) [C1]
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2004 |
Asquith KL, Baleato R, McLaughlin EA, Nixon B, Aitken RJ, 'Tyrosine phosphorylation activates surface chaperones facilitating sperm-zona recognition', Journal of Cell Science, 117 3645-3657 (2004) [C1]
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Nova |
2004 |
Aitken RJ, Koopman P, Lewis SEM, 'Seeds of concern', Nature, 432 48-52 (2004) [C1]
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Nova |
2004 |
Aitken RJ, Baker MA, O'Bryan M, 'Shedding Light on Chemiluminescence: The Application of Chemiluminescence in Diagnostic Andrology', Journal of Andrology, 25 455-465 (2004) [C1]
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2004 |
Baker MA, Aitken RJ, 'The importance of redox regulated pathways in sperm cell biology', Molecular and Cellular Endocrinology, 216 47-54 (2004) [C1]
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2004 |
Vernet P, Aitken RJ, Drevet JR, 'Antioxidant strategies in the epididymis', Molecular and Cellular Endocrinology, 216 31-39 (2004) [C1]
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2004 |
Curry BJ, Su H, Law EG, McLaughlin EA, Nixon B, Aitken RJ, 'Identification of RARhoGAP, a novel putative RhoGAP gene expressed in male germ cells', Genomics, 84 406-418 (2004) [C1]
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2004 |
Curry BJ, Roman SD, Wallace C, Scott RJ, Miriami E, Aitken RJ, 'Identification and characterisation of a novel splice variant of mouse and rat cytochrome b5/cytochrome b5 reductase', Genomics, 83 425-438 (2004) [C1]
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Nova |
2004 |
Aitken RJ, Ryan AL, Baker MA, McLaughlin EA, 'Redox activity associated with the maturation and capacitation of mammalian spermatozoa', Free Radical Biology and Medicine, 36 994-1010 (2004) [C1]
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2004 |
Li FQ, Aitken R, 'Cloning of porcine scFv antibodies by phage display and expression in Escherichia coli', VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY, 97 39-51 (2004)
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2004 |
Aitken RJ, 'The Founder's Lecture: Human spermatozoa, fruits of creation, seeds of doubt', Reproduction Fertility and Development, 16 655-664 (2004) [C1]
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2004 |
Aitken RJ, Baker MA, 'Oxidative stress and male reproductive biology', Reproduction, Fertility and Development, 16 581-588 (2004) [C1]
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2004 |
Sidhu KS, Mate KE, Gunasekera T, Veal D, Hetherington L, Baker MA, et al., 'A flow cytometric assay for global estimation of tyrosine phosphorylation associated with capacitation of spermatozoa from two marsupial species, the tammar wallaby (Macropus eugenii) and the brushtail possum (Trichosurus vulpecula)', Reproduction, 127 95-103 (2004) [C1]
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2004 |
Bennetts LE, Lin M, Aitken RJ, 'Cyclic AMP-Dependent Tyrosine Phosphorylation in Tammar Wallaby (Macropus eugenii) Spermatozoa', Journal of Experimental Zoology. Part A: Comparative Experimental Zoology, 301A 118-130 (2004) [C1]
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2003 |
Aitken RJ, Ryan AL, Curry BJ, Baker MA, 'Multiple forms of redox activity in populations of human spermatozoa', Molecular Human Reproduction, 9 645-661 (2003) [C1]
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2003 |
Sawyer DE, Mercer BG, Wiklendt AM, Aitken RJ, 'Quantitative analysis of gene-specific DNA damage in human spermatozoa', Mutation Research, 21-34 (2003) [C1]
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2003 |
Wade MA, Jones RC, Murdoch RN, Aitken RJ, 'Motility activation and second messenger signalling in spermatozoa from rat cauda epididymidis', Reproduction, 175-183 (2003) [C1]
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2003 |
McLaughlin EA, Holland MK, Aitken RJ, 'Contraceptive vaccines', Expert Opinion on Biological Therapy, 3 829-841 (2003) [C1]
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2003 |
Aitken RJ, Baker MA, Sawyer DE, 'Oxidative stress in the male germ line and its role in the aetiology of male infertility and genetic disease', Reproductive BioMedicine, 7 65-70 (2003) [C1]
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2003 |
Ecroyd HW, Jones RC, Aitken RJ, 'Tyrosine Phosphorylation of HSP-90 During Mammalian Sperm Capacitation', Biology of Reproduction, 1801-1807 (2003) [C1]
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2003 |
Ecroyd HW, Jones RC, Aitken RJ, 'Endogenous Redox Activity in Mouse Spermatozoa and Its Role in Regulating the Tyrosine Phosphorylation Events Associated with Sperm Capacitation', Biology of Reproduction, 347-354 (2003) [C1]
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2003 |
Baker MA, Krutskikh A, Aitken RJ, 'Biochemical entities involved in reactive oxygen species generation by human spermatozoa', Protoplasma, 145-151 (2003) [C1]
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2003 |
Aitken RJ, 'Reactive oxygen species generation by human spermatozoa: Reply', International Journal of Andrology, 26 127 (2003) [C3]
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2003 |
Wade MA, Roman SD, Jones RC, Aitken RJ, 'Adenylyl cyclase isoforms in rat testis and spermatozoa from the cauda epididymidis', Cell and Tissue Research, 314 411-419 (2003) [C1]
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2003 |
Baker MA, Lewis B, Hetherington L, Aitken RJ, 'Development of the Signalling Pathways Associated With Sperm Capacitation During Epididymal Maturation', Molecular Reproduction and Development, 446-457 (2003) [C1]
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2002 |
Aitken RJ, Marshall Graves H, 'The future of sex', Nature, 415 963 (2002) [C1]
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2002 |
Aitken RJ, Baker MA, 'Reactive oxygen species generation by human spermatozoa: a continuing enigma', International Journal of Andrology, 25 191-194 (2002) [C1]
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2002 |
Aitken RJ, 'Immunocontraceptive vaccines for human use', Journal of Reproductive Immunology, 57 273-287 (2002) [C1]
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2002 |
Lin M, Hess R, Aitken RJ, 'Induction of sperm maturation in vitro in epididymal cell cultures of the tammar wallaby (Macropus eugenii): disruption of motility initiation and sperm morphogenesis by inhibition of actin polymerization', Reproduction, 124 107-117 (2002) [C1]
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Nova |
2002 |
Paterson M, Jennings ZA, Wilson MR, Aitken RJ, 'the contraceptive potential of ZP3 and ZP3 peptides in a primate model.', Journal of Reproductive Immunology, 53 99-107 (2002) |
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Nova |
2001 |
Brown GK, Martin A, Roberts TK, Aitken RJ, 'Detection of Ehrlichia platys in dogs in Australia', Australian Veterinary Journal, 79 23-27 (2001) [C1]
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Nova |
2001 |
Lewis B, Aitken RJ, 'Impact of epididymal maturation on the tyrosine phosphorylation patterns exhibited by rat spermatozoa', Biology of Reproduction, 64 1545-1556 (2001) [C1]
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2001 |
Vernet P, Fulton N, Wallace C, Aitken RJ, 'Analysis of reactive oxygen species generating systems in rat epididymal spermatozoa', Biology of Reproduction, 65 1102-1113 (2001) [C1]
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2001 |
Scarlett CJ, Lin M, Aitken RJ, 'Actin polymerisation during morphogenesis of the acrosome as spermatozoa undergo epididymal maturation in the tammar wallaby (Macropus eugenii)', Journal of Anatomy, 198 93-101 (2001) [C1]
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Nova |
2001 |
Lewis B, Aitken RJ, 'A redox-regulated tyrosine phosphorylation cascade in rat spermatozoa', Journal of Andrology, 22 No. 4 611-622 (2001) [C1]
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2001 |
Aitken RJ, Krausz C, 'Oxidative stress, DNA damage and the Y chromosome', Reproduction, 122 497-506 (2001) [C1]
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2001 |
Sawyer DE, Roman SD, Aitken RJ, 'Relative susceptibilities of mitochondrial and nuclear DNA to damage induced by hydrogen peroxide in two mouse germ cell lines', Redox Report, 6 No. 3 182-184 (2001) [C1]
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2001 |
Aitken RJ, Baker HWG, Barat CLR, Behre HM, Comhaire F, Cooper TG, et al., 'WHO laboratory manual for the examination human sperm fluid and the interaction between spermatozoids and cervical mucus', Annali dell'Istituto Superiore di Sanita, 37 (2001) |
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2000 |
Eidne KA, Henery CC, Aitken RJ, 'Selection of peptides targeting the human sperm surface using random peptide phage display identify ligands homologous to ZP3', BIOLOGY OF REPRODUCTION, 63 1396-1402 (2000) [C1]
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2000 |
Kervancioglu ME, Saridogan E, Aitken RJ, Djahanbakhch O, 'Importance of sperm-to-epithelial cell contact for the capacitation of human spermatozoa in fallopian tube epithelial cell cocultures', FERTILITY AND STERILITY, 74 NO. 4 780-784 (2000) [C1]
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2000 |
Aitken RJ, Buckingham DW, Richardson D, Gardiner JC, Irvine DS, 'Impact of deep saturation dive on semen quality', INTERNATIONAL JOURNAL OF ANDROLOGY, 23 116-120 (2000) [C1]
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2000 |
Aitken RJ, Buckingham D, Richardson D, Gardiner JC, Irvine DS, 'Impact of a deep saturation dive on semen quality (vol 23, pg 116, 2000)', INTERNATIONAL JOURNAL OF ANDROLOGY, 23 253-253 (2000) |
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2000 |
Aitken RJ, 'Possible redox regulation of sperm motility activation', JOURNAL OF ANDROLOGY, 21, NO. 4 491-496 (2000) [C1]
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2000 |
Irvine DS, Twigg JP, Gordon EL, Fulton N, Milne PA, Aitken RJ, 'DNA integrity in human spermatozoa: Relationships with semen quality', JOURNAL OF ANDROLOGY, 21 33-44 (2000)
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2000 |
De Sawyer, Aitken RJ, 'Male-mediated developmental defects and childhood disease', Reproductive Medicine Review, 8 107-126 (2000)
Germline mutations have a tremendous impact on public health, since such damage is heritable, and thus transcends generations, as opposed to somatic mutations, which are confined ... [more]
Germline mutations have a tremendous impact on public health, since such damage is heritable, and thus transcends generations, as opposed to somatic mutations, which are confined to the individual (Figure 3). As recently as the mid-1980s, the prevailing view was that the male parent was an unlikely source of developmental defects. In the past decade, however, this notion has been seriously challenged. Several epidemiological studies demonstrate that paternal exposures can lead to germline mutations and a variety of developmental pathologies in offspring including spontaneous abortion, birth defects and childhood cancer. Currently, there is increasing concern regarding the declining health of the human male reproductive system, and the possibility of environmental toxicants as a major culprit. There is evidence that suggests a progressive decline in overall semen quality in recent decades, at least in Northern Europe. Sperm counts appear to be falling, and abnormal morphology is increasingly common. Other reproductive pathologies such as testicular cancer, hypospadias and cryptorchidism have risen with alarming rates in many highly industrialized areas, while cancers of the female reproductive tract (cervix, uterus, and ovarian) have remained relatively constant. In particular, men who work and live in heavily polluted areas have a greater tendency to sire offspring that die in utero, who are malformed, or who are at increased risk of developing childhood cancers. Moreover, sperm chromosome abnormalities, pregnancy loss and birth defects occur with much higher frequency in humans than in other species (Table 2). Because the severity of these pathologies appears to have increased within the last few decades, environmental factors are believed to be primarily responsible. Whether germline mutations in males are also increasing remains to be seen. The majority of birth defects (60-70%) are of unknown aetiology, and most human conceptions do not proceed to term. Smoking, poor semen quality (increased ROS levels from abnormal sperm and leukocytes), varicocele, infection, etc. can cause oxidative DNA damage in germ cells, and may be the 'missing link' in the aetiology of male germline mutations and paternally mediated developmental toxicity. The mechanisms by which environmental factors might stimulate mutations in the male germ line are not currently understood. However an exciting possibility that is consistent with our current understanding of the pathophysiology of male infertility is that oxidative stress is a key factor in the aetiology of such damage. ROS are clearly generated in excessive amounts by the spermatozoa of male patients, particularly those exhibiting a defective semen profile. This increase in free radical generation is associated with lipid peroxidation, oxidative base damage and DNA fragmentation, characterized by single- and double-strand breaks. It is known that spermatozoa exhibiting significant levels of oxidatively induced DNA fragmentation are capable of fertilization, and even severe levels of DNA fragmentation can be tolerated when ICSI is used to achieve conception. It is possible that the DNA fragmentation induced by oxidative stress can lead to a variety of human pathologies from early onset cancers to the induction of infertility itself. A correlation between DNA fragmentation in the male germline and childhood cancer in the offspring has already been observed in smokers. In addition, DNA fragmentation in spermatozoa may be the cause of the Y-chromosome deletions that are currently thought to underpin around 14% of all cases of severe oligo- or azoospermia. This hypothesis raises a large number of fundamental questions concerning the mechanisms responsible for creating oxidative stress in the male germline, the impact of such stress on the integrity of DNA in spermatozoa and the repair of such damage during early embryonic development. The clinical consequences of such damage in terms of...
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2000 |
Paterson M, Jennings ZA, van Duin M, Aitken RJ, 'Immunocontraception with zone pellucida proteins', CELLS TISSUES ORGANS, 166 228-232 (2000)
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2000 |
Lin M, Zhang X, Murdoch RN, Aitken RJ, 'In vitro culture of brushtail possum (Trichosurus vulpecula) epididymal epithelium and induction of epididymal sperm maturation in co-culture', Journal of Reproduction and Fertility, 119 1-14 (2000) [C1]
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2000 |
Mark D, Aitken RJ, Beaumont G, Witschger O, Lidén G, Koch W, et al., 'Development of a novel calibration tool for workplace aerosol samplers - Review of progress of EU project', Journal of Aerosol Science, 31 (2000)
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2000 |
Jones AD, Aitken R, Maynard A, Riediger G, Sahle W, Liden G, 'Thoracic sampling of fibres', Journal of Aerosol Science, 31 (2000)
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2000 |
Aitken RJ, Buckingham D, Richardson D, Gardiner JC, Irvine DS, 'Erratum: Impact of a deep saturation dive on semen quality (International Journal of Andrology (2000) 23 (116-120))', International Journal of Andrology, 23 253 (2000)
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1999 |
Cherrie JW, Aitken RJ, 'Measurement of human exposure to biologically relevant fractions of inhaled aerosols', Occupational and Environmental Medicine, 56 747-752 (1999)
Aerosol sampling has evolved with changes in technology and our understanding of the importance of assessing the biologically relevant fractions of the total aerosol. During the p... [more]
Aerosol sampling has evolved with changes in technology and our understanding of the importance of assessing the biologically relevant fractions of the total aerosol. During the past decade there has been international agreement on the definitions for the inhalable, thoracic, and respirable fractions and instruments have been developed to collect samples according to these conventions. These measurement techniques are now well established in the workplace and are increasingly being applied to assessments of nonoccupational exposure, as the practical difficulties in obtaining samples over 24 hours have been solved. It is argued that multiple aerosol size fractions should be measured for hazardous substances - such as inorganic lead - in which inhaled material may either be absorbed in the alveoli (respirable) or cleared from the ciliated airways in the lung to the gut and then absorbed (thoracic and extrathoracic). Such measurements should improve the evaluation of the risk for inhaled lead, particularly for non-occupational exposure of children. Also, passive aerosol sampling techniques may enable measurements of non-occupational exposure to be made over several weeks and this would also help improve the reliability of the risk evaluation for aerosol toxicants with a long biological half life. However, further development of these techniques is required if they are to be practicable and biologically relevant.
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1999 |
Lewis BA, Aitken RJ, 'The regulation of tyrosine phosphorylation in rat epididymal spermatozoa by calcium', BIOLOGY OF REPRODUCTION, 60 146-147 (1999) |
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1999 |
Vernet P, Fulton N, Aitken RJ, 'Identification of two independent O(2)(- center dot)generating systems in rat epididymal spermatozoa.', BIOLOGY OF REPRODUCTION, 60 206-206 (1999) |
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1999 |
Young IE, Nawroz IM, Aitken RJ, 'Phaeochromocytoma of the spermatic cord', JOURNAL OF CLINICAL PATHOLOGY, 52 305-306 (1999)
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1999 |
Aitken RJ, 'The Amoroso Lecture - The human spermatozoon - a cell in crisis?', JOURNAL OF REPRODUCTION AND FERTILITY, 115 1-7 (1999)
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1999 |
Scobie GA, Kerr LE, MacDuff P, Aitken RJ, 'Cloning, sequencing and site of origin of the rat sperm receptor protein, ZP3', ZYGOTE, 7 27-+ (1999)
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1999 |
Paterson M, Wilson MR, Jennings ZA, van Duin M, Aitken RJ, 'Design and evaluation of a ZP3 peptide vaccine in a homologous primate model.', Mol Hum Reprod, 5 342-352 (1999)
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1998 |
Aitken RJ, Harkiss D, Knox W, Paterson M, Irvine S, 'On the cellular mechanisms by which the bicarbonate ion mediates the extragenomic action of progesterone on human spermatozoa', BIOLOGY OF REPRODUCTION, 58 186-196 (1998)
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1998 |
Aitken RJ, Vernet P, 'Maturation of redox regulatory mechanisms in the epididymis', JOURNAL OF REPRODUCTION AND FERTILITY, 109-118 (1998)
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1998 |
Gomez E, Irvine DS, Aitken RJ, 'Evaluation of a spectrophotometric assay for the measurement of malondialdehyde and 4-hydroxyalkenals in human spermatozoa: relationships with semen quality and sperm function', INTERNATIONAL JOURNAL OF ANDROLOGY, 21 81-94 (1998)
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1998 |
Kerr LE, Paterson M, Aitken RJ, 'Molecular basis of sperm-egg interaction and the prospects for immunocontraception', JOURNAL OF REPRODUCTIVE IMMUNOLOGY, 40 103-118 (1998)
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1998 |
Twigg J, Fulton N, Gomez E, Irvine DS, Aitken RJ, 'Analysis of the impact of intracellular reactive oxygen species generation on the structural and functional integrity of human spermatozoa: lipid peroxidation, DNA fragmentation and effectiveness of antioxidants', HUMAN REPRODUCTION, 13 1429-1436 (1998)
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1998 |
Aitken RJ, Lowrie SJR, 'Measurements of the physical sampling efficiency of bioaerosol samplers', Journal of Aerosol Science, 29 (1998)
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1998 |
Twigg J, Irvine DS, Houston P, Fulton N, Michael L, Aitken RJ, 'Iatrogenic DNA damage induced in human spermatozoa during sperm preparation: protective significance of seminal plasma', MOLECULAR HUMAN REPRODUCTION, 4 439-445 (1998)
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1998 |
Paterson M, Wilson MR, Morris KD, van Duin M, Aitken RJ, 'Evaluation of the contraceptive potential of recombinant human ZP3 and human ZP3 peptides in a primate model: Their safety and efficacy', AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY, 40 198-209 (1998)
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1998 |
Kerr LE, Paterson M, Aitken RJ, 'Review: Molecular basis of sperm-egg interaction and the prospects', Journal of Reproductive Immunology, 40 103-118 (1998) |
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1998 |
Aitken RJ, Gordon E, Harkiss D, Twigg JP, Milne P, Jennings Z, Irvine DS, 'Relative impact of oxidative stress on the functional competence and genomic integrity of human spermatozoa', BIOLOGY OF REPRODUCTION, 59 1037-1046 (1998)
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1998 |
Aitken RJ, Harkiss D, Knox W, Paterson M, Irvine DS, 'A novel signal transduction cascade in capacitating human spermatozoa characterised by a redox-regulated, cAMP-mediated induction of tyrosine phosphorylation', JOURNAL OF CELL SCIENCE, 111 645-656 (1998)
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1998 |
Twigg JP, Irvine DS, Aitken RJ, 'Oxidative damage to DNA in human spermatozoa does not preclude pronucleus formation at intracytoplasmic sperm injection', HUMAN REPRODUCTION, 13 1864-1871 (1998)
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1997 |
Aitken RJ, 'Molecular mechanisms regulating human sperm function', MOLECULAR HUMAN REPRODUCTION, 3 169-173 (1997)
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1997 |
Allan IW, Irvine DS, Macnamee M, Aitken RJ, 'Field trial of a diluent for the transportation of human semen at ambient temperatures', FERTILITY AND STERILITY, 67 348-354 (1997)
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1997 |
Aitken RJ, 'Need: A special medline - For prior to 1966 - Reply', FERTILITY AND STERILITY, 68 181-181 (1997) |
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1997 |
Fisher HM, Aitken RJ, 'Comparative analysis of the ability of precursor germ cells and epididymal spermatozoa to generate reactive oxygen metabolites', JOURNAL OF EXPERIMENTAL ZOOLOGY, 277 390-400 (1997)
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1997 |
Kenny LC, Aitken R, Chalmers C, Fabriès JF, Gonzalez-Fernandez E, Kromhout H, et al., 'A collaborative european study of personal inhalable aerosol sampler performance', Annals of Occupational Hygiene, 41 135-153 (1997)
Following the adoption of new international sampling conventions for inhalable, thoracic and respirable aerosol fractions, a working group of Comite European de Normalisation (CEN... [more]
Following the adoption of new international sampling conventions for inhalable, thoracic and respirable aerosol fractions, a working group of Comite European de Normalisation (CEN) drafted a standard for the performance of workplace aerosol sampling instruments. The present study was set up to verify the experimental, statistical and mathematical procedures recommended in the draft performance standard and to check that they could be applied to inhalable aerosol samplers. This was achieved by applying the tests to eight types of personal inhalable aerosol sampler commonly used for workplace monitoring throughout Europe. The study led to recommendations for revising the CEN draft standard, in order to simplify the tests and reduce their cost. However, some further work will be needed to develop simpler test facilities and methods. Several of the samplers tested were found to perform adequately with respect to the inhalable sampling convention, at least over a limited range of typical workplace conditions. In general the samplers were found to perform best in low external wind speeds, which are the test conditions thought to be closest to those normally found in indoor workplaces. The practical implementation of the CEN aerosol sampling conventions requires decisions on which sampling instruments to use, estimation of the likely impact that changing sampling methods could have on apparent exposures, and adjustment where necessary of exposure limit values. The sampler performance data obtained in this project were affected by large experimental errors, but are nevertheless a useful input to decisions on how to incorporate the CEN inhalable sampling convention into regulation, guidance and occupational hygiene practice.
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1997 |
Aitken RJ, Fisher HM, Fulton N, Gomez E, Knox W, Lewis B, Irvine S, 'Reactive oxygen species generation by human spermatozoa is induced by exogenous NADPH and inhibited by the flavoprotein inhibitors diphenylene iodonium and quinacrine', MOLECULAR REPRODUCTION AND DEVELOPMENT, 47 468-482 (1997)
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1997 |
Flaherty SP, Michalowska J, Swann NJ, Dmowski WP, Matthews CD, Aitken RJ, 'Albumin gradients do not enrich Y-bearing human spermatozoa', HUMAN REPRODUCTION, 12 938-942 (1997)
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1997 |
Aitken RJ, 'The extragenomic action of progesterone on human spermatozoa', HUMAN REPRODUCTION, 12 38-42 (1997)
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1997 |
Aitken RJ, 'Sex steroids and fertilization', HUMAN REPRODUCTION, 12 S5-S5 (1997) |
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1997 |
Aitken RJ, 'Free oxygen radicals and human sperm function', HUMAN REPRODUCTION, 12 O27-O27 (1997) |
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1997 |
Twigg JP, Irvine DS, Aitken RJ, 'Exposure of human spermatozoa to reactive oxygen species enhances chromatin cross-linking and stimulates DNA strand breakage', HUMAN REPRODUCTION, 12 O118-O118 (1997) |
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1997 |
Aitken RJ, 'How to select viable spermatozoa for ICSI', HUMAN REPRODUCTION, 12 O129-O129 (1997) |
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1997 |
Paterson M, Wilson MR, vanDuin M, Aitken RJ, 'Evaluation of the contraceptive potential of antibodies to an immunodominant marmoset ZP3 peptide which recognize human zona pellucida', HUMAN REPRODUCTION, 12 P129-P129 (1997) |
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1997 |
Kerr LE, Brindle JP, Saxty BA, vanDuin M, Aitken RJ, 'Identification of novel sperm antigens with potential as immunocontraceptives', HUMAN REPRODUCTION, 12 P130-P130 (1997) |
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1997 |
MacDuff PE, Kerr LE, Aitken RJ, 'Molecular characterization of the zona pellucida glycoproteins ZP1, ZP2 and ZP3 in the rat', HUMAN REPRODUCTION, 12 P163-P163 (1997) |
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1997 |
Knox W, Harkiss HDJ, Irvine DS, Aitken RJ, 'Impact of tyrosine phosphorylation status on the generation of calcium transients in human spermatozoa following exposure to progesterone', HUMAN REPRODUCTION, 12 P164-P164 (1997) |
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1997 |
Fulton N, Fisher H, Paterson M, Irvine DS, Aitken RJ, 'Purification and characterization of NADPH oxidase in human spermatozoa', HUMAN REPRODUCTION, 12 P165-P165 (1997) |
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1997 |
vanDuin M, Grootenhuis A, Bunschoten H, Aitken RJ, 'Production and characterization of monoclonal antibodies against human ZP3 that inhibit human sperm-zona pellucida binding', HUMAN REPRODUCTION, 12 R127-R127 (1997) |
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1997 |
Rossi AG, Aitken RJ, 'Interactions between leukocytes and the male reproductive system: The unanswered questions', FATE OF THE MALE GERM CELL, 424 245-252 (1997)
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1997 |
Aitken RJ, 'The cell biology of fertilization', FATE OF THE MALE GERM CELL, 424 291-299 (1997)
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1997 |
Sakka E, Rae M, Aitken J, Bramley T, 'Protein kinase C- and Ca2+ ionophore-stimulated production of reactive oxygen species in mechanically dispersed isolated bovine luteal cells', BIOLOGY OF REPRODUCTION, 57 428-435 (1997)
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1997 |
Fisher HM, Aitken RJ, 'Comparative analysis of the ability of precursor germ cells and epididymal spermatozoa to generate reactive oxygen metabolites', Journal of Experimental Zoology, 277 390-400 (1997)
Male germ cells at various stages of differentiation from pachytene spermatocytes to mature caudal epididymal spermatozoa were examined for their ability to generate reactive oxyg... [more]
Male germ cells at various stages of differentiation from pachytene spermatocytes to mature caudal epididymal spermatozoa were examined for their ability to generate reactive oxygen species (ROS) using sensitive chemiluminescence techniques. In general, spermatozoa were found to spontaneously generate hydrogen peroxide as they progressed through the epididymis, maximal activity being observed on the release of mature cells from the caudal region into a modified Krebs-Ringer's solution. The spontaneous production of hydrogen peroxide rose rapidly during the first 10 min after the spermatozoa had been diluted into culture medium and thereafter stabilized, neither phorbol esters nor A23187 subsequently influencing this activity. Low levels of superoxide generation were also detected in suspensions of epididymal spermatozoa, but did not correlate with maturation status. However, superoxide production could be dramatically enhanced by the addition of exogenous NADPH, in a manner that was closely correlated with the stage of epididymal development being maximal for immature cells recovered from the caput epididymis in all species. Precursor germ cells (pachytene spermatocytes, round and elongate spermatids) similarly generated chemiluminescent signals compatible with the low level generation of ROS. Superoxide generation in these cells could again be stimulated by NADPH, via mechanisms that were inversely related to the stage of germ cell differentiation, the greatest activity being observed in pachytene spermatocytes. These results demonstrate that differentiating male germ cells have the potential to generate ROS, and have implications for the redox regulation of gonadal function and the development of reproductive pathologies involving oxidative stress.
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1997 |
Aitken RJ, 'The extragenomic action of progesterone on human spermatozoa', Human Reproduction, 12 38-42 (1997)
Progesterone exerts an extragenomic action on human spermatozoa, including a calcium transient in the acrosomal domain of these cells via putative receptor sites located on the sp... [more]
Progesterone exerts an extragenomic action on human spermatozoa, including a calcium transient in the acrosomal domain of these cells via putative receptor sites located on the sperm surface. The calcium transient can be visualized in >80% of spermatozoa treated with this steroid although their capacity to respond to the calcium signal is heavily dependent on their capacitation status. Capacitation is, in turn, dependent on a redox-regulated increase in tyrosine phosphorylation that primes the spermatozoa in such a way that the primary calcium influx induced by progesterone is followed by a chloride efflux and the onset of secondary calcium oscillations. The way in which these changes precipitate a biological response and the nature of the molecular pathways orchestrating these events are currently unknown.
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1997 |
Saxty B, Aitken J, 'Sperm surface antigens and the prospects for contraceptive vaccine development', Reproductive Medicine Review, 6 81-95 (1997)
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1997 |
Aitken RJ, Fisher HM, Fulton N, Gomez E, Knox W, Lewis B, Irvine S, 'Reactive oxygen species generation by human spermatozoa is induced by exogenous NADPH and inhibited by the flavoprotein inhibitors diphenylene iodonium and quinacrine', Molecular Reproduction and Development, 47 468-482 (1997)
Human spermatozoa possess a specialized capacity to generate reactive oxygen species (ROS) that is thought to be of significance in the redox regulation of sperm capacitation (De ... [more]
Human spermatozoa possess a specialized capacity to generate reactive oxygen species (ROS) that is thought to be of significance in the redox regulation of sperm capacitation (De Lamirande and Gagnon, 1993; Aitken et al., 1995). However, the mechanisms by which ROS are generated by these cells are not understood. In this study we have examined the possible significance of NADPH as a substrate for ROS production by human spermatozoa. Addition of NADPH to viable populations of motile spermatozoa induced a sudden dose- dependent increase in the rate of superoxide generation via mechanisms that could not be disrupted by inhibitors of the mitochondrial electron transport chain (antimycin A, rotenone, carbonyl cyanide m-chlorophenylhydrazone [CCCP], and sodium azide), diaphorase (dicoumarol) xanthine oxidase (allopurinol), or lactic acid dehydrogenase (sodium oxamate). However, NADPH- induced ROS generation could be stimulated by permeabilization and was negatively correlated with sperm function. Both NADH and NADPH were active electron donors in this system, while NAD+ and NADP+ exhibited little activity. Stereospecificity was evident in the response in that only the ß- isomer of NADPH supported superoxide production. The involvement of a flavoprotein in the electron transfer process was indicated by the high sensitivity of the oxidase to inhibition by diphenylene iodonium and quinacrine. These results indicate that NAD(P)H can serve as an electron donor for superoxide generation by human spermatozoa and present a simple strategy for the production of motile populations of free radical generating cells with which to study the significance of these molecules in the control of normal and pathological sperm function.
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1997 |
Aitken RJ, 'The extragenomic action of progesterone on human spermatozoa', Journal of the British Fertility Society, 2 38-42 (1997)
Progesterone exerts an extragenomic action on human spermatozoa, including a calcium transient in the acrosomal domain of these cells via putative receptor sites located on the sp... [more]
Progesterone exerts an extragenomic action on human spermatozoa, including a calcium transient in the acrosomal domain of these cells via putative receptor sites located on the sperm surface. The calcium transient can be visualized in >80% of spermatozoa treated with this steroid although their capacity to respond to the calcium signal is heavily dependent on their capacitation status. Capacitation is, in turn, dependent on a redox-regulated increase in tyrosine phosphorylation that primes the spermatozoa in such a way that the primary calcium influx induced by progesterone is followed by a chloride efflux and the onset of secondary calcium oscillations. The way in which these changes precipitate a biological response and the nature of the molecular pathways orchestrating these events are currently unknown.
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1997 |
Saxty B, Aitken J, 'Sperm surface antigens and the prospects for contraceptive vaccine development', Reproductive Medicine Review, 6 81-95 (1997)
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1996 |
Aitken RJ, Buckingham DW, Irvine DS, 'The extragenomic action of progesterone on human spermatozoa: Evidence for a ubiquitous response that is rapidly down-regulated', ENDOCRINOLOGY, 137 3999-4009 (1996)
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1996 |
Baker HWG, Brindle J, Irvine DS, Aitken RJ, 'Protective effect of antioxidants on the impairment of sperm motility by activated polymorphonuclear leukocytes', FERTILITY AND STERILITY, 65 411-419 (1996)
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1996 |
Aitken RJ, Irvine DS, 'Reproductive biology - Fertilization without sperm', NATURE, 379 493-& (1996)
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1996 |
Gomez E, Buckingham DW, Brindle J, Lanzafame F, Irvine DS, Aitken RJ, 'Development of an image analysis system to monitor the retention of residual cytoplasm by human spermatozoa: Correlation with biochemical markers of the cytoplasmic space, oxidative stress, and sperm function', JOURNAL OF ANDROLOGY, 17 276-287 (1996)
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1996 |
Sukcharoen N, Keith J, Irvine DS, Aitken RJ, 'Prediction of the in-vitro fertilization (IVF) potential of human spermatozoa using sperm function tests: The effect of the delay between testing and IVF', HUMAN REPRODUCTION, 11 1030-1034 (1996)
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1996 |
Aitken RJ, Allan IW, Irvine DS, Macnamee M, 'Studies on the development of diluents for the transportation and storage of human semen at ambient temperature', HUMAN REPRODUCTION, 11 2186-2196 (1996)
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1996 |
Aitken RJ, Buckingham DW, Harkiss D, Paterson M, Fisher H, Irvine DS, 'The extragenomic action of progesterone on human spermatozoa is influenced by redox regulated changes in tyrosine phosphorylation during capacitation', MOLECULAR AND CELLULAR ENDOCRINOLOGY, 117 83-93 (1996)
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1996 |
Paterson M, Wilson MR, vanDuin M, Aitken RJ, 'Evaluation of zona pellucida antigens as potential candidates for immunocontraception', ZONA PELLUCIDA GLYCOPROTEINS AND IMMUNOCONTRACEPTION, 175-182 (1996)
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1996 |
Aitken RJ, Buckingham DW, Carreras A, Irvine DS, 'Superoxide dismutase in human sperm suspensions: Relationship with cellular composition, oxidative stress, and sperm function', FREE RADICAL BIOLOGY AND MEDICINE, 21 495-504 (1996)
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1996 |
Aitken RJ, Paterson M, vanDuin M, 'The potential of the zona pellucida as a target for immunocontraception', AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY, 35 175-180 (1996)
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1996 |
Aitken RJ, Buckingham DW, West K, Brindle J, 'On the use of paramagnetic beads and ferrofluids to assess and eliminate the leukocytic contribution to oxygen radical generation by human sperm suspensions', AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY, 35 541-551 (1996)
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1996 |
Irvine S, Cawood E, Richardson D, MacDonald E, Aitken RJ, 'Evidence of deteriorating semen quality in the United Kingdom: birth cohort study in 577 men in Scotland over 11 years', British Medical Journal, 312 467-471 (1996)
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Nova |
1996 |
Gomez E, Aitken RJ, 'Impact of IVF-ET culture media on peroxidative damage to human spermatozoa.', Fertility and sterility, 65 880-882 (1996) |
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1996 |
Gomez E, Aitken J, 'Impact of in vitro fertilization culture media on peroxidative damage to human spermatozoa', FERTILITY AND STERILITY, 65 880-882 (1996)
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1995 |
Aitken RJ, 'The bioassay of sperm function', Fertility and Sterility, 63 1134-1134 (1995) |
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1995 |
Aitken RJ, 'Male infertility, prognostic value of old and new tests', Reviews in Assisted Conception, 5 26-31 (1995) |
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1995 |
Aitken RJ, Buckingham DW, Brindle J, Gomez E, Baker HWG, Irvine DS, 'Andrology: Analysis of sperm movement in relation to the oxidative stress created by leukocytes in washed sperm preparations and seminal plasma', Human Reproduction, 10 2061-2071 (1995)
The addition of luminol to unprocessed semen samples resulted in the generation of chemiluminescent signals, the intensity of which was highly correlated with the level of leukocy... [more]
The addition of luminol to unprocessed semen samples resulted in the generation of chemiluminescent signals, the intensity of which was highly correlated with the level of leukocyte contamination. Despite the spontaneous oxidant-generating capacity of seminal leukocytes, no correlations were observed between leukocyte contamination and the fertility status of the subjects or any aspect of the semen profile, including the motility of the spermatozoa or their performance in a hyaluronate penetration assay. Luminol-dependent chemiluminescence and leukocyte contamination were also correlated in washed sperm suspensions prepared either by repeated centrifugation or on discontinuous Percoll gradients. However, in such sperm suspensions, the spontaneous generation of oxidants by contaminating leukocytes (>2×104 leukocytes/ml) was invariably associated with a decreased capacity for movement. Moreover, causative associations between leukocyte contamination, reactive oxygen species generation, lipid peroxidation and impaired sperm motility were revealed by experiments involving the selective addition or removal of activated leukocytes. From these observations we can conclude that low concentrations of leukocytes are a common feature of the human ejaculate and can impair sperm function, particularly in the absence of seminal plasma. These findings have implications for our understanding of the importance of leukocytospermia in defining the fertility of human spermatozoa in vivo and in vitro. © 1995 Oxford University Press.
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1995 |
WHITE DR, CLARKSON JS, RATNASOORIYA WD, AITKEN RJ, 'COMPLEMENTARY EFFECTS OF PROPRANOLOL AND NONOXYNOL-9 UPON HUMAN SPERM MOTILITY', CONTRACEPTION, 52 241-247 (1995)
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1995 |
AITKEN RJ, 'FUTURE OF THE HAMSTER OOCYTE PENETRATION ASSAY - REPLY', FERTILITY AND STERILITY, 63 1134-1135 (1995) |
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1995 |
SUKCHAROEN N, KEITH J, IRVINE DS, AITKEN RJ, 'PREDICTING THE FERTILIZING POTENTIAL OF HUMAN SPERM SUSPENSIONS IN-VITRO - IMPORTANCE OF SPERM MORPHOLOGY AND LEUKOCYTE CONTAMINATION', FERTILITY AND STERILITY, 63 1293-1300 (1995)
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1995 |
AITKEN RJ, PATERSON M, FISHER H, BUCKINGHAM DW, VANDUIN M, 'REDOX REGULATION OF TYROSINE PHOSPHORYLATION IN HUMAN SPERMATOZOA AND ITS ROLE IN THE CONTROL OF HUMAN SPERM FUNCTION', JOURNAL OF CELL SCIENCE, 108 2017-2025 (1995)
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1995 |
AITKEN RJ, 'THE COMPLEXITIES OF CONCEPTION', SCIENCE, 269 39-40 (1995)
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1995 |
AITKEN RJ, BAKER HWG, IRVINE DS, 'ON THE NATURE OF SEMEN QUALITY AND INFERTILITY', HUMAN REPRODUCTION, 10 248-249 (1995)
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1995 |
AITKEN RJ, BAKER HWG, 'SEMINAL LEUKOCYTES - PASSENGERS, TERRORISTS OR GOOD-SAMARITANS', HUMAN REPRODUCTION, 10 1736-1739 (1995)
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1995 |
AITKEN RJ, BUCKINGHAM DW, BRINDLE J, GOMEZ E, BAKER HWG, IRVINE DS, 'ANALYSIS OF SPERM MOVEMENT IN RELATION TO THE OXIDATIVE STRESS CREATED BY LEUKOCYTES IN WASHED SPERM PREPARATIONS AND SEMINAL PLASMA', HUMAN REPRODUCTION, 10 2061-2071 (1995)
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1995 |
IRVINE DS, CAWOOD EHH, RICHARDSON DW, MACDONALD E, AITKEN RJ, 'A SURVEY OF SEMEN DONATION - PHASE-II THE VIEW OF THE DONORS', HUMAN REPRODUCTION, 10 2752-2753 (1995)
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1995 |
Sukcharoen N, Keith J, Irvine DS, Aitken RJ, 'Definition of the optimal criteria for identifying hyperactivated human spermatozoa at 25 Hz using in-vitro fertilization as a functional end-point', HUMAN REPRODUCTION, 10 2928-2937 (1995)
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1994 |
Aitken J, Buckingham D, Harkiss D, 'Andrology: Analysis of the extent to which sperm movement can predict the results of ionophore-enhanced functional assays of the acrosome reaction and sperm-oocyte fusion', Human Reproduction, 9 1867-1874 (1994)
This study has examined the extent to which the information generated by ionophore-enhanced bioassays of the acrosome reaction and sperm-oocyte fusion might be predicted from the ... [more]
This study has examined the extent to which the information generated by ionophore-enhanced bioassays of the acrosome reaction and sperm-oocyte fusion might be predicted from the computer-aided analysis of sperm motility Strong correlations (r ~ 0.7) were observed between specific components of sperm movement in semen and the potential for A23187-induced sperm-oocyte fusion, generating a stepwise regression coefficient of R = 0.663 on the basis of two criteria, percentage progressive motility and amplitude of sperm lateral head displacement (ALH). The movement characteristics of the spermatozoa recovered from the Percoll gradients gave an even higher R value of 0.838 on the basis of four variables (percentage rapid, average path velocity, straightness and ALH). In contrast, the ability of human spermatozoa to undergo acrosome reaction in response to A23187 exhibited a limited correlation with sperm movement, whether these measurements were made in the original semen sample or following Percoll purification (R ~ 0.4). These results have diagnostic implications, since sperm-oocyte fusion and the acrosome reaction clearly differ in their relative dependence on sperm motility. In practical terms, it should be noted that the computer-aided analysis of sperm movement was shown to provide up to 70% of the information generated by the more laboured assessment of sperm-oocyte fusion. © 1994 Oxford University Press.
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1994 |
VANDUIN M, POLMAN JEM, DEBREET ITM, VANGINNEKEN K, BUNSCHOTEN H, GROOTENHUIS A, et al., 'RECOMBINANT HUMAN ZONA-PELLUCIDA PROTEIN ZP3 PRODUCED BY CHINESE-HAMSTER OVARY CELLS INDUCES THE HUMAN SPERM ACROSOME REACTION AND PROMOTES SPERM-EGG FUSION', BIOLOGY OF REPRODUCTION, 51 607-617 (1994)
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1994 |
HOKKE CH, DAMM JBL, PENNINKHOF B, AITKEN RJ, KAMERLING JP, VLIEGENTHART JFG, 'STRUCTURE OF THE O-LINKED CARBOHYDRATE CHAINS OF PORCINE ZONA-PELLUCIDA GLYCOPROTEINS', EUROPEAN JOURNAL OF BIOCHEMISTRY, 221 491-512 (1994)
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1994 |
KERVANCIOGLU ME, DJAHANBAKHCH O, AITKEN RJ, 'EPITHELIAL-CELL COCULTURE AND THE INDUCTION OF SPERM CAPACITATION', FERTILITY AND STERILITY, 61 1103-1108 (1994)
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1994 |
KRAUSZ C, MILLS C, ROGERS S, TAN SL, AITKEN RJ, 'STIMULATION OF OXIDANT GENERATION BY HUMAN SPERM SUSPENSIONS USING PHORBOL ESTERS AND FORMYL PEPTIDES - RELATIONSHIPS WITH MOTILITY AND FERTILIZATION IN-VITRO', FERTILITY AND STERILITY, 62 599-605 (1994)
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1994 |
AITKEN RJ, WEST K, BUCKINGHAM D, 'LEUKOCYTIC INFILTRATION INTO THE HUMAN EJACULATE AND ITS ASSOCIATION WITH SEMEN QUALITY, OXIDATIVE STRESS, AND SPERM FUNCTION', JOURNAL OF ANDROLOGY, 15 343-352 (1994)
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1994 |
IRVINE DS, AITKEN RJ, 'SEMINAL FLUID ANALYSIS AND SPERM FUNCTION TESTING', ENDOCRINOLOGY AND METABOLISM CLINICS OF NORTH AMERICA, 23 725-748 (1994)
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1994 |
AITKEN RJ, 'A FREE-RADICAL THEORY OF MALE-INFERTILITY', REPRODUCTION FERTILITY AND DEVELOPMENT, 6 19-24 (1994)
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1994 |
AITKEN RJ, 'PATHOPHYSIOLOGY OF HUMAN SPERMATOZOA', CURRENT OPINION IN OBSTETRICS & GYNECOLOGY, 6 128-135 (1994)
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1994 |
Irvine DS, Aitken RJ, 'Falling sperm quality', BMJ: British Medical Journal, 309 476-476 (1994) |
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1994 |
Aitken RJ, Fisher H, 'Reactive oxygen species generation and human spermatozoa, the balance of benefit and risk', BioEssays, 16 259-268 (1994)
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1994 |
Aitken RJ, 'The future of the hamster oocyte penetration assay', Fertility and Sterility, 62 17-19 (1994)
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1994 |
Aitken RJ, buckingham D, Harkiss D, 'Analysis of the extent to which sperm movement can predict the results of ionophore-enhanced functional assays of the acrosome reaction and sperm-oocyte fusion', Human Reproduction, 9 1867-1874 (1994)
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1994 |
Aitken RJ, Krausz C, Buckingham DW, 'Relationships between biochemical markers for residual sperm cytoplasm, reactive oxygen species generation and the presence of leucocytes and precursor germ cells in human sperm suspensions', Molecular Reproduction and Development, 39 268-279 (1994) |
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1993 |
Aitken RJ, Howie RM, Clapp R, '11 O 01 Dilution sampler for evaluation of airborne fibre concentrations in asbetos stripping enclosures', Journal of Aerosol Science, 24 (1993)
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1993 |
Aitken RJ, Vincent JH, Mark D, 'Application of porous foams as size selectors for biologically relevant samplers', Applied Occupational and Environmental Hygiene, 8 363-369 (1993)
In recent years it has become accepted that dust sampling instrumentation used to assess airborne exposure to airborne contaminants should sample according to biologically relevan... [more]
In recent years it has become accepted that dust sampling instrumentation used to assess airborne exposure to airborne contaminants should sample according to biologically relevant criteria. This article describes progress toward the development of a personal sampling instrument which uses porous foams to select the thoracic and respirable subfractions of the inhalable fraction of total airborne particulate. The new instrument is based on the Institute of Occupational Medicine personal inhalable aerosol sampler and comprises an inhalable entry and two selection foams in series between the entry and the collection filter. Measurements of the foam penetration characteristics and the reproducibility of these characteristics have been carried out. In most cases different samples of the same grade of foam have been shown to be reproducible in their selection characteristics. Where this is not the case a simple quality control method, based on measurements of pressure drop, has been identified. Aitken, R.J.; Vincent, J.H.; Mark, D>Application of Porous Foams as Size Selectors for Biologically Relevant Samplers. © 1993 Taylor & Francis Group, LLC.
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1993 |
AITKEN RJ, PATERSON M, KOOTHAN PT, 'CONTRACEPTIVE VACCINES', BRITISH MEDICAL BULLETIN, 49 88-99 (1993)
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1993 |
Aitken RJ, Brindle JP, 'Andrology: Analysis of the ability of three probes targeting the outer acrosomal membrane or acrosomal contents to detect the acrosome reaction in human spermatozoa', Human Reproduction, 8 1663-1669 (1993)
A comparative analysis has been undertaken of the behaviour of probes targeting the outer acrosomal membrane (Arachis hypogaea lectin) or constituents of the acrosomal vesicle (Pi... [more]
A comparative analysis has been undertaken of the behaviour of probes targeting the outer acrosomal membrane (Arachis hypogaea lectin) or constituents of the acrosomal vesicle (Pisum sativum lectin or monoclonal antibody CRB9) following the induction of the acrosome reaction in human spermatozoa with the ionophore A23187. The results obtained with these two classes of reagent were highly correlated (r = ~0.8), although the absolute rates of acrosome reaction were significantly different; the probe targeting the outer acrosomal membrane (A.hypogaea) consistently gave higher results than either of the probes targeting the acrosomal contents. Time-dependent studies, employing a doublelabelling technique, indicated that this difference was apparent from the earliest time point examined (15 min after A23187 addition) and reflected the more rapid dissipation of the A.hypogaea lectin from the acrosomal region of the cell than either of the probes targeting the acrosomal vesicle. These results indicate that the outer acrosomal membrane is dispersed from acrosome-reacting human spermatozoa more rapidly than certain major constituents of the acrosomal vesicle and have possible implications for the design of diagnostic assays focusing on this aspect of human sperm function. © 1993 Oxford University Press.
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1993 |
AITKEN RJ, BUCKINGHAM D, HARKISS D, 'USE OF A XANTHINE-OXIDASE FREE-RADICAL GENERATING-SYSTEM TO INVESTIGATE THE CYTOTOXIC EFFECTS OF REACTIVE OXYGEN SPECIES ON HUMAN SPERMATOZOA', JOURNAL OF REPRODUCTION AND FERTILITY, 97 441-450 (1993)
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1993 |
AITKEN RJ, HARKISS D, BUCKINGHAM D, 'RELATIONSHIP BETWEEN IRON-CATALYZED LIPID-PEROXIDATION POTENTIAL AND HUMAN SPERM FUNCTION', JOURNAL OF REPRODUCTION AND FERTILITY, 98 257-265 (1993)
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1993 |
AITKEN RJ, BUCKINGHAM DW, FANG HG, 'ANALYSIS OF THE RESPONSES OF HUMAN SPERMATOZOA TO A23187 EMPLOYING A NOVEL TECHNIQUE FOR ASSESSING THE ACROSOME REACTION', JOURNAL OF ANDROLOGY, 14 132-141 (1993)
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1993 |
AITKEN RJ, BRINDLE JP, 'ANALYSIS OF THE ABILITY OF 3 PROBES TARGETING THE OUTER ACROSOMAL MEMBRANE OR ACROSOMAL CONTENTS TO DETECT THE ACROSOME REACTION IN HUMAN SPERMATOZOA', HUMAN REPRODUCTION, 8 1663-1669 (1993)
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1993 |
Thillai-Koothan P, van Duin M, Aitken RJ, 'Cloning, sequencing and oocyte-specific expression of the marmoset sperm receptor protein, ZP3', ZYGOTE, 1 93-101 (1993)
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1993 |
AITKEN RJ, HARKISS D, BUCKINGHAM DW, 'ANALYSIS OF LIPID-PEROXIDATION MECHANISMS IN HUMAN SPERMATOZOA', MOLECULAR REPRODUCTION AND DEVELOPMENT, 35 302-315 (1993)
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1993 |
Van Duin M, Polman JEM, Verkoelen CEH, Aitken RJ, 'Cloning and characterization of the human sperm receptor ligand ZP3, evidence for a second polymorphic allele with a different frequency in the Caucasian and Japanese populations', Genomics, 14 1064-1070 (1993) |
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1992 |
Tomlinson M, Kessopoulou E, Barratt C, Aitken RJ, 'Criteria for the diagnosis of infertility [2]', American Journal of Obstetrics and Gynecology, 166 264-265 (1992) |
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1992 |
Vincent JH, Aitken RJ, Mark D, 'Further studies of porous plastic foam filtration media', Journal of Aerosol Science, 23 627-630 (1992)
Available data for the particle penetration characteristics of plastic foam media are used to develop a semi-empirical model which can be applied in the development of practical p... [more]
Available data for the particle penetration characteristics of plastic foam media are used to develop a semi-empirical model which can be applied in the development of practical particle size-selective sampling instruments. © 1992.
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1992 |
AITKEN RJ, 'CRITERIA FOR THE DIAGNOSIS OF INFERTILITY - REPLY', AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY, 166 264-265 (1992)
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1992 |
PATERSON M, KOOTHAN PT, MORRIS KD, OBYRNE KT, BRAUDE P, WILLIAMS A, AITKEN RJ, 'ANALYSIS OF THE CONTRACEPTIVE POTENTIAL OF ANTIBODIES AGAINST NATIVE AND DEGLYCOSYLATED PORCINE ZP3 INVIVO AND INVITRO', BIOLOGY OF REPRODUCTION, 46 523-534 (1992)
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1992 |
AITKEN RJ, FORREST APM, CHETTY U, ROBERTS MM, HUGGINS A, MACDONALD HL, et al., 'ASSESSMENT OF NONPALPABLE MAMMOGRAPHIC ABNORMALITIES - COMPARISON BETWEEN SCREENING AND SYMPTOMATIC CLINICS', BRITISH JOURNAL OF SURGERY, 79 925-927 (1992)
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1992 |
WU FCW, AITKEN RJ, 'SPERM MORPHOLOGY - PREDICTIVE VALUE - REPLY', FERTILITY AND STERILITY, 57 942-943 (1992)
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1992 |
KRAUSZ C, WEST K, BUCKINGHAM D, AITKEN RJ, 'DEVELOPMENT OF A TECHNIQUE FOR MONITORING THE CONTAMINATION OF HUMAN SEMEN SAMPLES WITH LEUKOCYTES', FERTILITY AND STERILITY, 57 1317-1325 (1992)
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1992 |
AITKEN RJ, BUCKINGHAM DW, WEST KM, 'REACTIVE OXYGEN SPECIES AND HUMAN SPERMATOZOA - ANALYSIS OF THE CELLULAR MECHANISMS INVOLVED IN LUMINOL-DEPENDENT AND LUCIGENIN-DEPENDENT CHEMILUMINESCENCE', JOURNAL OF CELLULAR PHYSIOLOGY, 151 466-477 (1992)
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1992 |
AITKEN RJ, BUCKINGHAM D, WEST K, WU FC, ZIKOPOULOS K, RICHARDSON DW, 'DIFFERENTIAL CONTRIBUTION OF LEUKOCYTES AND SPERMATOZOA TO THE GENERATION OF REACTIVE OXYGEN SPECIES IN THE EJACULATES OF OLIGOZOOSPERMIC PATIENTS AND FERTILE DONORS', JOURNAL OF REPRODUCTION AND FERTILITY, 94 451-462 (1992)
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1992 |
AITKEN RJ, BUCKINGHAM D, 'ENHANCED DETECTION OF REACTIVE OXYGEN SPECIES PRODUCED BY HUMAN SPERMATOZOA WITH 7-DIMETHYL AMINO-NAPHTHALIN-1, 2-DICARBONIC ACID HYDRAZIDE', INTERNATIONAL JOURNAL OF ANDROLOGY, 15 211-219 (1992)
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1992 |
WALLACE EM, AITKEN RJ, WU FCW, 'RESIDUAL SPERM FUNCTION IN OLIGOZOOSPERMIA INDUCED BY TESTOSTERONE ENANTHATE ADMINISTERED AS A POTENTIAL STEROID MALE CONTRACEPTIVE', INTERNATIONAL JOURNAL OF ANDROLOGY, 15 416-424 (1992)
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1992 |
AITKEN RJ, BOWIE H, BUCKINGHAM D, HARKISS D, RICHARDSON DW, WEST KM, 'SPERM PENETRATION INTO A HYALURONIC-ACID POLYMER AS A MEANS OF MONITORING FUNCTIONAL COMPETENCE', JOURNAL OF ANDROLOGY, 13 44-54 (1992)
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1992 |
Aitken RJ, 'Reply', American Journal of Obstetrics and Gynecology, 166 264-265 (1992)
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1992 |
VANDUIN M, POLMAN JEM, VERKOELEN C, BUNSCHOTEN H, MEYERINK JH, OLIJVE W, AITKEN RJ, 'CLONING AND CHARACTERIZATION OF THE HUMAN SPERM RECEPTOR LIGAND ZP3 - EVIDENCE FOR A 2ND POLYMORPHIC ALLELE WITH A DIFFERENT FREQUENCY IN THE CAUCASIAN AND JAPANESE POPULATIONS', GENOMICS, 14 1064-1070 (1992)
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1992 |
Aitken RJ, 'A family of fusion proteins', Nature, 196-197 (1992)
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1991 |
AITKEN RJ, IRVINE DS, WU FC, 'PROSPECTIVE ANALYSIS OF SPERM-OOCYTE FUSION AND REACTIVE OXYGEN SPECIES GENERATION AS CRITERIA FOR THE DIAGNOSIS OF INFERTILITY', AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY, 164 542-551 (1991)
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1991 |
Aitken RJ, 'The 17th Jennifer Hallam Memorial Lecture: Immunocontraception', British Journal of Family Planning, 16 3-7 (1991) |
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1991 |
SANCHEZ R, TOEPFERPETERSEN E, AITKEN RJ, SCHILL WB, 'A NEW METHOD FOR EVALUATION OF THE ACROSOME REACTION IN VIABLE HUMAN SPERMATOZOA', ANDROLOGIA, 23 197-203 (1991) [C1]
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1991 |
Aitken RJ, 'Human spermatozoa in assisted reproduction', International Journal of Andrology, 14 75-75 (1991)
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1991 |
Aitken RJ, 'Do sperm find eggs attractive?', Nature, 19-20 (1991)
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1991 |
Aitken RJ, 'A clue to Kartageners', Nature, 306-306 (1991)
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1991 |
Aitken J, 'Reproductive biology. A clue to Kartagener's.', Nature, 353 306 (1991)
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1990 |
AITKEN RJ, 'EVALUATION OF HUMAN SPERM FUNCTION', BRITISH MEDICAL BULLETIN, 46 654-674 (1990)
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1990 |
AITKEN RJ, 'CONTRACEPTIVE VACCINE DEVELOPMENT', EUROPEAN JOURNAL OF PHARMACOLOGY, 183 13-13 (1990)
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1990 |
AITKEN RJ, KERR L, BOLTON V, HARGREAVE T, 'ANALYSIS OF SPERM FUNCTION IN GLOBOZOOSPERMIA - IMPLICATIONS FOR THE MECHANISM OF SPERM-ZONA INTERACTION', FERTILITY AND STERILITY, 54 701-707 (1990)
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1990 |
WU FCW, AITKEN RJ, FERGUSON A, 'EFFECT OF SULFASALAZINE ON SPERMATOGENESIS - REPLY', FERTILITY AND STERILITY, 54 747-748 (1990) |
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1990 |
AITKEN RJ, WEST KM, 'ANALYSIS OF THE RELATIONSHIP BETWEEN REACTIVE OXYGEN SPECIES PRODUCTION AND LEUKOCYTE INFILTRATION IN FRACTIONS OF HUMAN SEMEN SEPARATED ON PERCOLL GRADIENTS', INTERNATIONAL JOURNAL OF ANDROLOGY, 13 433-451 (1990)
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1990 |
NASRESFAHANI M, JOHNSON MH, AITKEN RJ, 'THE EFFECT OF IRON AND IRON CHELATORS ON THE INVITRO BLOCK TO DEVELOPMENT OF THE MOUSE PREIMPLANTATION EMBRYO - BAT6 A NEW MEDIUM FOR IMPROVED CULTURE OF MOUSE EMBRYOS INVITRO', HUMAN REPRODUCTION, 5 997-1003 (1990)
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1990 |
Aitken RJ, 'Cell biology of defective sperm function', Cell Biology International Reports, 33 (1990) |
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1990 |
AITKEN RJ, 'DEVELOPMENT OF INVITRO TESTS OF HUMAN SPERM FUNCTION - A DIAGNOSTIC-TOOL AND MODEL SYSTEM FOR TOXICOLOGICAL ANALYSES', TOXICOLOGY IN VITRO, 4 560-569 (1990)
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1990 |
PATERSON M, AITKEN RJ, 'DEVELOPMENT OF VACCINES TARGETING THE ZONA-PELLUCIDA', CURRENT OPINION IN IMMUNOLOGY, 2 743-747 (1990)
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1990 |
BRAUDE P, JOHNSON MH, AITKEN RJ, 'HUMAN FERTILIZATION AND EMBRYOLOGY BILL GOES TO REPORT STAGE', BRITISH MEDICAL JOURNAL, 300 1410-1412 (1990)
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1990 |
Braude P, Johnson MH, Aitken RJ, 'Human Fertilisation and Embryology Bill goes to report stage. Donor anonymity to go? Is GIFT included?', British Medical Journal, 300 1410-1412 (1990)
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1990 |
Paterson M, Aitken RJ, 'Development of vaccines targeting the zona pellucida', Current Opinions in immunology, 743-747 (1990) |
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1990 |
Nasr-Esfahani M, Aitken RJ, 'The measurement of H202 levels in preimplantation embryos from blocking and non-blocking strains of mice', Development, 501-507 (1990) |
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1990 |
Aitken RJ, 'Viable offspring', Nature, 588-588 (1990) |
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1990 |
NASRESFAHANI MH, AITKEN JR, JOHNSON MH, 'HYDROGEN-PEROXIDE LEVELS IN MOUSE OOCYTES AND EARLY CLEAVAGE STAGE EMBRYOS DEVELOPED INVITRO OR INVIVO', DEVELOPMENT, 109 501-507 (1990)
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1989 |
Aitken RJ, Vincent JH, Mark D, 'Model studies to investigate the nature of air flow in mines. (1989)
Scale model studies for investigation of ventilation problems in mines have not been used with confidence due to the complex aerodynamics of a longwall coalface. This article desc... [more]
Scale model studies for investigation of ventilation problems in mines have not been used with confidence due to the complex aerodynamics of a longwall coalface. This article describes use of the 'tracer-decay' method to examine dispersion of airborne material travelling along a faceline in 1/10-scale and full-scale longwall systems. For both cases longitudinal dispersion (non-dimensionalized with respect to linear scale and velocity) was found to be independent of Reynolds number. In addition however, an unexpected relationship with longitudinal dimension was observed which implied that the dispersion could not be explained in terms of turbulent diffusion alone; an additional process, possibly related to the entrainment of material within the coherent wake structures behind the legs of roof supports, is postulated. Examination of the results overall suggests that scaling is possible between full and 1/10-scale provided that the mean wind speed exceeds about 2 m/s.
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1989 |
AITKEN RJ, CLARKSON JS, FISHEL S, 'GENERATION OF REACTIVE OXYGEN SPECIES, LIPID-PEROXIDATION, AND HUMAN-SPERM FUNCTION', BIOLOGY OF REPRODUCTION, 41 183-197 (1989)
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1989 |
WU FCW, AITKEN RJ, FERGUSON A, 'INFLAMMATORY BOWEL-DISEASE AND MALE-INFERTILITY - EFFECTS OF SULFASALAZINE AND 5-AMINOSALICYLIC ACID ON SPERM-FERTILIZING CAPACITY AND REACTIVE OXYGEN SPECIES GENERATION', FERTILITY AND STERILITY, 52 842-845 (1989)
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1989 |
WU FCW, AITKEN RJ, 'SUPPRESSION OF SPERM FUNCTION BY DEPOT MEDROXYPROGESTERONE ACETATE AND TESTOSTERONE ENANTHATE IN STEROID MALE CONTRACEPTION', FERTILITY AND STERILITY, 51 691-698 (1989)
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1989 |
AITKEN RJ, GAZE MN, RODGER A, CHETTY U, FORREST APM, 'ARM MORBIDITY WITHIN A TRIAL OF MASTECTOMY AND EITHER NODAL SAMPLE WITH SELECTIVE RADIOTHERAPY OR AXILLARY CLEARANCE', SURGERY, 106 A13-A16 (1989) |
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1989 |
WHITE DR, AITKEN RJ, 'INFLUENCE OF EPIDIDYMAL MATURATION ON CYCLIC-AMP LEVELS IN HAMSTER SPERMATOZOA', INTERNATIONAL JOURNAL OF ANDROLOGY, 12 29-43 (1989)
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1989 |
AITKEN RJ, 'THE ROLE OF FREE OXYGEN RADICALS AND SPERM FUNCTION', INTERNATIONAL JOURNAL OF ANDROLOGY, 12 95-97 (1989)
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1989 |
AITKEN RJ, 'The role of free oxygen radicals and sperm function', International Journal of Andrology, 12 95-97 (1989)
Despite the fact that defective sperm function is the largest defined cause of human infertility there are few, if any, effective treatments which address this condition. The majo... [more]
Despite the fact that defective sperm function is the largest defined cause of human infertility there are few, if any, effective treatments which address this condition. The major reason for this lack of therapeutic options, is our lack of knowledge concerning the precise biochemical nature of the lesions that are responsible for the loss of fertilizing potential. Without a knowledge of the mechanisms leading to defective sperm function, a rational approach towards the development of appropriate therapies is clearly impossible. In this context, evidence for at least one major cause of sperm dysfunction has come from a number of recent studies suggesting a possible role for lipid peroxidation in the loss of functional competence. This concept was originally put forward by Jones & Mann 1973; Jones, Mann & Sherins, 1978;1979) and has since been supported by studies focusing on the capacity of human sperm to generate the reactive oxygen species which initiate the peroxidation process (Aitken & Clarkson, 1987; Alvarez et al., 1987).
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1989 |
WHITE DR, AITKEN RJ, 'RELATIONSHIP BETWEEN CALCIUM, CYCLIC-AMP, ATP, AND INTRACELLULAR PH AND THE CAPACITY OF HAMSTER SPERMATOZOA TO EXPRESS HYPERACTIVATED MOTILITY', GAMETE RESEARCH, 22 163-177 (1989)
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1989 |
RATNASOORIYA WD, AITKEN RJ, 'TRANSMEMBRANE MIGRATION TECHNIQUE - REEXAMINATION OF ITS USEFULNESS IN SPERM MOTILITY ASSESSMENTS', JOURNAL OF PHARMACOLOGICAL METHODS, 22 259-264 (1989)
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1989 |
AITKEN RJ, CLARKSON JS, HARGREAVE TB, IRVINE DS, WU FCW, 'ANALYSIS OF THE RELATIONSHIP BETWEEN DEFECTIVE SPERM FUNCTION AND THE GENERATION OF REACTIVE OXYGEN SPECIES IN CASES OF OLIGOZOOSPERMIA', JOURNAL OF ANDROLOGY, 10 214-220 (1989)
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1989 |
West JD, West KM, Aitken RJ, 'Detection of Y-bearing spermatozoa by DNA-DNA in situ hybridisation', Molecular Reproduction and Development, 1 201-207 (1989)
In situ hybridisation of a Y chromosome-specific DNA probe to preparations of decondensed spermatozoa revealed approximately 46.7% labelled spermatozoa among 3,900 scored. This is... [more]
In situ hybridisation of a Y chromosome-specific DNA probe to preparations of decondensed spermatozoa revealed approximately 46.7% labelled spermatozoa among 3,900 scored. This is not significantly different from the 50% expected if only the Y chromosome-bearing spermatozoa are hybridised. Control hybridisations of Escherichia coli DNA and salmon testis DNA to decondensed sperm produced no significant labelling, whereas more than 99% of the spermatozoa were heavily labelled after hybridisation to total human DNA. These controls indicate that the methodology described in this paper renders the chromatin accessible for hybridisation and that the 50% hybridisation observed with the Y chromosome DNA probe was specific. In situ hybridisation with the Y probe therefore identifies the Y-bearing spermatozoa, and the protocol described should prove useful in evaluating methods of separating Y-bearing and X-bearing spermatozoa. Copyright © 1988 Wiley-Liss, Inc.
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1989 |
Braude P, Johnson MH, Aitken RJ, 'Positive benefits of IVF', Lancet, 1328-1329 (1989) |
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1989 |
BRAUDE P, JOHNSON M, AITKEN J, 'BENEFITS OF INVITRO FERTILIZATION', LANCET, 2 1328-1329 (1989)
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1988 |
AITKEN RJ, PARSLOW JM, HARGREAVE TB, HENDRY WF, 'INFLUENCE OF ANTISPERM ANTIBODIES ON HUMAN-SPERM FUNCTION', BRITISH JOURNAL OF UROLOGY, 62 367-373 (1988)
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1988 |
HARGREAVE TB, AITKEN RJ, ELTON RA, 'PROGNOSTIC-SIGNIFICANCE OF THE ZONA-FREE HAMSTER EGG TEST', BRITISH JOURNAL OF UROLOGY, 62 603-608 (1988)
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1988 |
AITKEN RJ, ELTON RA, 'POISSON-DISTRIBUTION OF SPERM AMONG OVA', FERTILITY AND STERILITY, 50 182-182 (1988) |
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1988 |
HENDERSON CJ, HULME MJ, AITKEN RJ, 'CONTRACEPTIVE POTENTIAL OF ANTIBODIES TO THE ZONA PELLUCIDA', JOURNAL OF REPRODUCTION AND FERTILITY, 83 325-343 (1988)
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1988 |
AITKEN RJ, PATERSON M, 'NEW HORIZONS IN CONTRACEPTION', NATURE, 335 492-493 (1988)
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1988 |
AITKEN RJ, CLARKSON JS, HULME MJ, HENDERSON CJ, 'ANALYSIS OF CALMODULIN ACCEPTOR PROTEINS AND THE INFLUENCE OF CALMODULIN ANTAGONISTS ON HUMAN-SPERMATOZOA', GAMETE RESEARCH, 21 93-111 (1988)
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1988 |
AITKEN RJ, CLARKSON JS, 'SIGNIFICANCE OF REACTIVE OXYGEN SPECIES AND ANTIOXIDANTS IN DEFINING THE EFFICACY OF SPERM PREPARATION TECHNIQUES', JOURNAL OF ANDROLOGY, 9 367-376 (1988)
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1988 |
AITKEN RJ, 'ASSESSMENT OF SPERM FUNCTION FOR IVF', HUMAN REPRODUCTION, 3 89-95 (1988)
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1988 |
AITKEN J, 'FUTURE-DEVELOPMENTS IN CONTRACEPTION', PRACTITIONER, 232 46-& (1988)
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1987 |
AITKEN RJ, HULME MJ, HENDERSON CJ, HARGREAVE TB, ROSS A, 'ANALYSIS OF THE SURFACE LABELING CHARACTERISTICS OF HUMAN-SPERMATOZOA AND THE INTERACTION WITH ANTISPERM ANTIBODIES', JOURNAL OF REPRODUCTION AND FERTILITY, 80 473-485 (1987)
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1987 |
AITKEN RJ, CLARKSON JS, 'CELLULAR BASIS OF DEFECTIVE SPERM FUNCTION AND ITS ASSOCIATION WITH THE GENESIS OF REACTIVE OXYGEN SPECIES BY HUMAN-SPERMATOZOA', JOURNAL OF REPRODUCTION AND FERTILITY, 81 459-469 (1987)
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1987 |
AITKEN RJ, 'SPERM SEPARATION TECHNIQUES', INTERNATIONAL JOURNAL OF ANDROLOGY, 10 643-645 (1987)
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1987 |
HENDERSON CJ, HULME MJ, AITKEN RJ, 'ANALYSIS OF THE BIOLOGICAL PROPERTIES OF ANTIBODIES RAISED AGAINST INTACT AND DEGLYCOSYLATED PORCINE ZONAE PELLUCIDAE', GAMETE RESEARCH, 16 323-341 (1987)
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1987 |
HENDERSON CJ, BRAUDE P, AITKEN RJ, 'POLYCLONAL ANTIBODIES TO A 32-KDA DEGLYCOSYLATED POLYPEPTIDE FROM PORCINE ZONAE PELLUCIDAE WILL PREVENT HUMAN GAMETE INTERACTION INVITRO', GAMETE RESEARCH, 18 251-265 (1987)
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1987 |
AITKEN RJ, THATCHER S, GLASIER AF, CLARKSON JS, WU FCW, BAIRD DT, 'RELATIVE ABILITY OF MODIFIED VERSIONS OF THE HAMSTER OOCYTE PENETRATION TEST, INCORPORATING HYPEROSMOTIC MEDIUM OR THE IONOPHORE A23187, TO PREDICT IVF OUTCOME', HUMAN REPRODUCTION, 2 227-231 (1987)
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1987 |
Irvine DS, Aitken RJ, 'Predictive value of in vitro sperm function tests in the context of an AID service', Human Reproduction, 539-545 (1987) |
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1986 |
AITKEN RJ, 'DIAGNOSIS OF HUMAN-SPERM FUNCTION', DEVELOPMENT GROWTH & DIFFERENTIATION, 28 9-10 (1986)
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1986 |
TEMPLETON AA, VANLOOK P, ANGELL RE, AITKEN RJ, LUMSDEN MA, BAIRD DT, 'OOCYTE RECOVERY AND FERTILIZATION RATES IN WOMEN AT VARIOUS TIMES AFTER THE ADMINISTRATION OF HCG', JOURNAL OF REPRODUCTION AND FERTILITY, 76 771-778 (1986)
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1986 |
AITKEN RJ, ELTON RA, 'APPLICATION OF POISSON-DISTRIBUTION THEORY TO THE ZONA-FREE HAMSTER OOCYTE PENETRATION TEST TO ASSESS SPERM FUNCTION OF MEN WITH ASTHENOZOOSPERMIA', JOURNAL OF REPRODUCTION AND FERTILITY, 77 67-74 (1986)
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1986 |
AITKEN RJ, IRVINE S, KELLY RW, 'SIGNIFICANCE OF INTRACELLULAR CALCIUM AND CYCLIC ADENOSINE-3',5'-MONOPHOSPHATE IN THE MECHANISMS BY WHICH PROSTAGLANDINS INFLUENCE HUMAN-SPERM FUNCTION', JOURNAL OF REPRODUCTION AND FERTILITY, 77 451-462 (1986)
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1986 |
AITKEN RJ, MATTEI A, IRVINE S, 'PARADOXICAL STIMULATION OF HUMAN-SPERM MOTILITY BY 2-DEOXYADENOSINE', JOURNAL OF REPRODUCTION AND FERTILITY, 78 515-527 (1986)
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1986 |
AITKEN RJ, ELTON RA, 'APPLICATION OF A POISSON-GAMMA-MODEL TO STUDY THE INFLUENCE OF GAMETE CONCENTRATION ON SPERM-OOCYTE FUSION IN THE ZONA-FREE HAMSTER EGG PENETRATION TEST', JOURNAL OF REPRODUCTION AND FERTILITY, 78 733-739 (1986)
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1986 |
AITKEN RJ, WAITES GMH, 'THE ZONA-FREE HAMSTER OOCYTE PENETRATION TEST AND THE DIAGNOSIS OF MALE-FERTILITY - PROCEEDINGS OF A WORKSHOP HELD IN BOSTON, MAY 3 1985 - PREFACE', INTERNATIONAL JOURNAL OF ANDROLOGY, 9-9 (1986) |
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1986 |
AITKEN RJ, ELTON RA, 'QUANTITATIVE-ANALYSIS OF SPERM-EGG INTERACTION IN THE ZONA-FREE HAMSTER EGG PENETRATION TEST', INTERNATIONAL JOURNAL OF ANDROLOGY, 14-30 (1986)
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1986 |
IRVINE DS, AITKEN RJ, 'CLINICAL-EVALUATION OF THE ZONA-FREE HAMSTER EGG PENETRATION TEST IN THE MANAGEMENT OF THE INFERTILE COUPLE - PROSPECTIVE AND RETROSPECTIVE STUDIES', INTERNATIONAL JOURNAL OF ANDROLOGY, 97-112 (1986)
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1986 |
WOLF DP, AITKEN RJ, ALEXANDER N, BARROS C, BLAQUIER JA, COMHAIRE F, et al., 'THE ZONA-FREE HAMSTER OOCYTE PENETRATION TEST AND THE DIAGNOSIS OF MALE-FERTILITY - PROCEEDINGS OF A WORKSHOP HELD IN BOSTON, MAY 3 1985 - GROUP DISCUSSION', INTERNATIONAL JOURNAL OF ANDROLOGY, 145-199 (1986) |
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1986 |
unknown, 'The zona-free hamster oocyte penetration test and the diagnosis of male fertility', International Journal of Andrology, Supplement, 0-0 (1986) |
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1986 |
IRVINE DS, AITKEN RJ, LEES MM, REID C, 'FAILURE OF HIGH INTRAUTERINE INSEMINATION OF HUSBANDS SEMEN', LANCET, 2 972-973 (1986)
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1986 |
IRVINE DS, AITKEN RJ, 'MEASUREMENT OF INTRACELLULAR CALCIUM IN HUMAN-SPERMATOZOA', GAMETE RESEARCH, 15 57-71 (1986)
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1986 |
AITKEN RJ, WARNER PE, REID C, 'FACTORS INFLUENCING THE SUCCESS OF SPERM CERVICAL-MUCUS INTERACTION IN PATIENTS EXHIBITING UNEXPLAINED INFERTILITY', JOURNAL OF ANDROLOGY, 7 3-10 (1986)
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1986 |
IRVINE DS, AITKEN RJ, 'PREDICTIVE VALUE OF INVITRO SPERM FUNCTION-TESTS IN THE CONTEXT OF AN AID SERVICE', HUMAN REPRODUCTION, 1 539-545 (1986)
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1986 |
Messinis IE, Templeton A, Angell R, Aitken RJ, 'A comparison of fixed regimes for obtaining human cleaving oocytes for research purposes', British Journal of Obstetrics and Gynaecology, 39-42 (1986) |
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1986 |
ANGELL RR, TEMPLETON AA, AITKEN RJ, 'CHROMOSOME-STUDIES IN HUMAN INVITRO FERTILIZATION', HUMAN GENETICS, 72 333-339 (1986)
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1986 |
FAOAGALI JL, TROUGHTON D, AITKEN J, GWYNNE JF, 'CAMPYLOBACTER-LIKE ORGANISMS IN GASTRIC BIOPSIES - A CHRISTCHURCH STUDY', NEW ZEALAND MEDICAL JOURNAL, 99 50-52 (1986)
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1986 |
Aitken RJ, Waites GMH, 'PREFACE', International Journal of Andrology, 9 9-9 (1986)
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1986 |
unknown, 'The zona-free hamster oocyte penetration test and the diagnosis of male fertility', International Journal of Andrology, Supplement, 0-0 (1986) |
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1985 |
IRVINE DS, AITKEN RJ, 'THE VALUE OF ADENOSINE-TRIPHOSPHATE (ATP) MEASUREMENTS IN ASSESSING THE FERTILIZING ABILITY OF HUMAN-SPERMATOZOA', FERTILITY AND STERILITY, 44 806-813 (1985)
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1985 |
AITKEN RJ, KELLY RW, 'ANALYSIS OF THE DIRECT EFFECTS OF PROSTAGLANDINS ON HUMAN-SPERM FUNCTION', JOURNAL OF REPRODUCTION AND FERTILITY, 73 139-146 (1985)
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1985 |
AITKEN RJ, SUTTON M, WARNER P, RICHARDSON DW, 'RELATIONSHIP BETWEEN THE MOVEMENT CHARACTERISTICS OF HUMAN-SPERMATOZOA AND THEIR ABILITY TO PENETRATE CERVICAL-MUCUS AND ZONA-FREE HAMSTER OOCYTES', JOURNAL OF REPRODUCTION AND FERTILITY, 73 441-449 (1985)
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1985 |
AITKEN RJ, 'DIAGNOSTIC-VALUE OF THE ZONA-FREE HAMSTER OOCYTE PENETRATION TEST AND SPERM MOVEMENT CHARACTERISTICS IN OLIGOZOOSPERMIA', INTERNATIONAL JOURNAL OF ANDROLOGY, 8 348-356 (1985)
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1985 |
Aitken RJ, Clarkson JS, Irvine DS, Richardson DW, 'Contribution of defective sperm function to infertility', Acta Europaea Fertilitatis, 16 273-280 (1985)
The nature of significance of some biologically orientated tests of human sperm function is discussed by the authors. In particular tests for motility and the fertilizing capacity... [more]
The nature of significance of some biologically orientated tests of human sperm function is discussed by the authors. In particular tests for motility and the fertilizing capacity of spermatozoa are examined, motility tests and the zona-free hamster oocyte penetration test are particularly criticized. The authors affirm that hamster oocyte tests carried out in the presence of A 23187 have shown to correlate with fertility in vivo and may be of critical value in decreasing the incidence of false negative scores. The data furnished by this test will provide a logical basis for discovering the etiology of male infertility as well as devising techniques to both detect and treat these conditions.
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1985 |
Westergaard L, Byskov AG, Van Look PFA, Angell R, Aitken RJ, 'Meiosis inducing substances in human preovulatory follicular fluid related to time of follicle aspiration and to the potential of the oocyte to fertilize and cleave in vitro', Fertility and Sterility, 663-667 (1985)
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1984 |
AITKEN RJ, BEST FSM, WARNER P, TEMPLETON A, 'A PROSPECTIVE-STUDY OF THE RELATIONSHIP BETWEEN SEMEN QUALITY AND FERTILITY IN CASES OF UNEXPLAINED INFERTILITY', JOURNAL OF ANDROLOGY, 5 297-303 (1984)
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1984 |
AITKEN RJ, ROSS A, HARGREAVE T, RICHARDSON D, BEST F, 'ANALYSIS OF HUMAN-SPERM FUNCTION FOLLOWING EXPOSURE TO THE IONOPHORE-A23187 - COMPARISON OF NORMOSPERMIC AND OLIGOZOOSPERMIC MEN', JOURNAL OF ANDROLOGY, 5 321-329 (1984)
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1984 |
SCHATS R, AITKEN RJ, TEMPLETON AA, DJAHANBAKHCH O, 'THE ROLE OF CERVICAL-MUCUS SEMEN INTERACTION IN INFERTILITY OF UNKNOWN ETIOLOGY', BRITISH JOURNAL OF OBSTETRICS AND GYNAECOLOGY, 91 371-376 (1984)
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1984 |
Aitken RJ, 'In vitro fertilization for male infertility', Acta Europaea Fertilitatis, 15 425-429 (1984)
Increasing attention has recently been focussed on the possibility of using the in vitro fertilization/embryo transfer procedure (IVF) in the treatment of male infertility. In thi... [more]
Increasing attention has recently been focussed on the possibility of using the in vitro fertilization/embryo transfer procedure (IVF) in the treatment of male infertility. In this paper the authors describe this application of IVF therapy with particular emphasis on the criteria employed for patient selection and pharmacological enhancement of sperm function.
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1984 |
AITKEN RJ, 'CLINICAL-ASSESSMENT OF HUMAN-SPERM FUNCTION', CONTRACEPTION FERTILITE SEXUALITE, 12 33-37 (1984)
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1984 |
Aitken RJ, 'Clinical assessment of human sperm function', Contraception Fertilite Sexualite, 12 33-37 (1984) |
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1984 |
RICHARDSON DW, AITKEN RJ, LOUDON NB, 'THE FUNCTIONAL COMPETENCE OF HUMAN-SPERMATOZOA RECOVERED AFTER VASECTOMY', JOURNAL OF REPRODUCTION AND FERTILITY, 70 575-579 (1984)
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1984 |
AITKEN RJ, ELTON RA, 'SIGNIFICANCE OF POISSON-DISTRIBUTION THEORY IN ANALYZING THE INTERACTION BETWEEN HUMAN-SPERMATOZOA AND ZONA-FREE HAMSTER OOCYTES', JOURNAL OF REPRODUCTION AND FERTILITY, 72 311-321 (1984)
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1984 |
AITKEN RJ, 'DIAGNOSTIC-VALUE OF THE HAMSTER OOCYTE PENETRATION ASSAY', INTERNATIONAL JOURNAL OF ANDROLOGY, 7 273-275 (1984)
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1984 |
TEMPLETON AA, GLASIER A, ANGELL RR, AITKEN RJ, 'WHAT POTENTIAL OVUM DONORS THINK', LANCET, 1 1081-1082 (1984)
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1983 |
AITKEN RJ, BEST F, RICHARDSON DW, SCHATS R, SIMM G, 'INFLUENCE OF CAFFEINE ON MOVEMENT CHARACTERISTICS, FERTILIZING-CAPACITY AND ABILITY TO PENETRATE CERVICAL-MUCUS OF HUMAN-SPERMATOZOA', JOURNAL OF REPRODUCTION AND FERTILITY, 67 19-27 (1983)
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1983 |
KHOLKUTE SD, AITKEN RJ, LUNN SF, 'PLASMA TESTOSTERONE RESPONSE TO HCG STIMULATION IN THE MALE MARMOSET MONKEY (CALLITHRIX-JACCHUS-JACCHUS)', JOURNAL OF REPRODUCTION AND FERTILITY, 67 457-463 (1983)
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1983 |
ANGELL RR, AITKEN RJ, VANLOOK PFA, LUMSDEN MA, TEMPLETON AA, 'CHROMOSOME-ABNORMALITIES IN HUMAN-EMBRYOS AFTER INVITRO FERTILIZATION', NATURE, 303 336-338 (1983)
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1983 |
AITKEN RJ, 'REPRODUCTIVE PHYSIOLOGY OF VERTEBRATES, 2ND EDITION - VANTEINHOVEN,A', NATURE, 305 165-166 (1983)
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1983 |
AITKEN RJ, LIU J, BEST FSM, RICHARDSON DW, 'AN ANALYSIS OF THE DIRECT EFFECTS OF GOSSYPOL ON HUMAN-SPERMATOZOA', INTERNATIONAL JOURNAL OF ANDROLOGY, 6 157-167 (1983)
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1983 |
Aitken RJ, Liu J, Best FSM, Richardson DW, 'An analysis of the direct effects of gossypol on human spermatozoa', International Journal of Andrology, 6 157-167 (1983)
The direct effects of gossypol and its acetic acid adduct, on the movement and functional competence of human spermatozoa were investigated employing exposure times of 1, 5 or 15 ... [more]
The direct effects of gossypol and its acetic acid adduct, on the movement and functional competence of human spermatozoa were investigated employing exposure times of 1, 5 or 15 min and concentrations of 50 µM, 500 µM and 1000 µM. These compounds markedly reduced the motility, velocity, frequency of sperm head rotation and linearity of sperm progression, the most significant effects being observed with gossypol acetic acid on populations of ¿capacitated¿ spermatozoa. Significant direct effects of gossypol on the ability of human spermatozoa to penetrate both cervical mucus and zona-free hamster ova were also observed, which were independent of any effects on motility. These results reinforce the notion that gossypol may serve a contraceptive role in the female as a ¿spermicidal¿ agent, and suggest that this compound may also be of scientific value as a probe for identifying and isolating functionally important components of the human spermatozoon. Copyright © 1983, Wiley Blackwell. All rights reserved
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1983 |
AITKEN RJ, 'CLINICAL-ASSESSMENT OF HUMAN-SPERM FUNCTION', JOURNAL OF OBSTETRICS AND GYNAECOLOGY, 3 S48-S52 (1983)
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1983 |
AITKEN RJ, ROSS A, LEES MM, 'ANALYSIS OF SPERM FUNCTION IN KARTAGENERS SYNDROME', FERTILITY AND STERILITY, 40 696-698 (1983)
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1983 |
AITKEN RJ, WANG YF, LIU J, BEST F, RICHARDSON DW, 'THE INFLUENCE OF MEDIUM COMPOSITION, OSMOLARITY AND ALBUMIN CONTENT ON THE ACROSOME REACTION AND FERTILIZING-CAPACITY OF HUMAN-SPERMATOZOA - DEVELOPMENT OF AN IMPROVED ZONA-FREE HAMSTER EGG PENETRATION TEST', INTERNATIONAL JOURNAL OF ANDROLOGY, 6 180-193 (1983)
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1983 |
AITKEN RJ, WARNER P, BEST FSM, TEMPLETON AA, DJAHANBAKHCH O, MORTIMER D, LEES MM, 'THE PREDICTABILITY OF SUBNORMAL PENETRATING CAPACITY OF SPERM IN CASES OF UNEXPLAINED INFERTILITY', INTERNATIONAL JOURNAL OF ANDROLOGY, 6 212-220 (1983)
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1982 |
AITKEN RJ, BEST FSM, RICHARDSON DW, DJAHANBAKHCH O, LEES MM, 'THE CORRELATES OF FERTILIZING-CAPACITY IN NORMAL FERTILE MEN', FERTILITY AND STERILITY, 38 68-76 (1982)
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1982 |
AITKEN RJ, BEST FSM, RICHARDSON DW, DJAHANBAKHCH O, MORTIMER D, TEMPLETON AA, LEES MM, 'AN ANALYSIS OF SPERM FUNCTION IN CASES OF UNEXPLAINED INFERTILITY - CONVENTIONAL CRITERIA, MOVEMENT CHARACTERISTICS, AND FERTILIZING-CAPACITY', FERTILITY AND STERILITY, 38 212-221 (1982)
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1982 |
AITKEN RJ, HOLME E, RICHARDSON DW, HULME M, 'PROPERTIES OF INTACT AND UNIVALENT (FAB) ANTIBODIES RAISED AGAINST ISOLATED, SOLUBILIZED, MOUSE ZONAE PELLUCIDAE', JOURNAL OF REPRODUCTION AND FERTILITY, 66 327-334 (1982)
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1982 |
HULME MJ, AITKEN RJ, RICHARDSON DW, 'USE OF A RADIOIMMUNOASSAY TECHNIQUE TO MEASURE THE TITER OF ANTI-COW-ZONA ANTIBODIES IN MARMOSET MONKEYS', GAMETE RESEARCH, 5 271-281 (1982)
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1982 |
Templeton AA, Aitken RJ, Mortimer D, Best F, 'Sperm function in patients with unexplained infertility', British Journal of Obstetrics and Gynaecology, 550-554 (1982) |
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1982 |
Aitken RJ, 'A review of the development and function of reproductive organs', Biochemical Transactions, 2 (1982) |
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1982 |
Aitken RJ, 'Family Planning II. Research and development in contraception', Journal of Postgraduate General Practice, 211-216 (1982) |
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1982 |
Aitken RJ, 'Seminal secrets', Nature, 633-643 (1982) |
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1982 |
Aitken RJ, Chisholm GD, 'The ascent of andrology', Nature, 499-499 (1982) |
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1982 |
Hannan WJ, Millar AM, Emmett PC, Aitken RJ, Love RG, Muir AL, 'Effective penetrance of the lung periphery using a dry monodisperse Tc99m aerosol', Nuclear Medicine Communications, 3 106 (1982) |
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1982 |
AITKEN RJ, BEST FSM, RICHARDSON DW, DJAHANBAKHCH O, TEMPLETON A, LEES MM, 'AN ANALYSIS OF SEMEN QUALITY AND SPERM FUNCTION IN CASES OF OLIGOZOOSPERMIA', FERTILITY AND STERILITY, 38 705-711 (1982)
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1982 |
HARDING RD, HULME MJ, LUNN SF, HENDERSON C, AITKEN RJ, 'PLASMA PROGESTERONE LEVELS THROUGHOUT THE OVARIAN CYCLE OF THE COMMON MARMOSET (CALLITHRIX-JACCHUS)', JOURNAL OF MEDICAL PRIMATOLOGY, 11 43-51 (1982)
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1981 |
DOR J, RUDAK E, AITKEN RJ, 'ANTISPERM ANTIBODIES - THEIR EFFECT ON THE PROCESS OF FERTILIZATION STUDIED INVITRO', FERTILITY AND STERILITY, 35 535-541 (1981)
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1981 |
RUDAK E, DOR J, AITKEN RJ, DJAHANBAKHCH O, BOWMAN P, WEISSENBERG R, et al., 'CONTRIBUTION OF ABNORMAL SPERM FUNCTION TO THE ETIOLOGY OF UNEXPLAINED INFERTILITY, AS DIAGNOSED BY THE ZONA-FREE HAMSTER EGG PENETRATION TEST', ISRAEL JOURNAL OF MEDICAL SCIENCES, 17 780-780 (1981) |
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1981 |
DOR J, RUDAK E, AITKEN RJ, 'INHIBITORY EFFECT OF ANTI-SPERM ANTIBODIES ON THE FERTILIZING-CAPACITY OF HUMAN-SPERMATOZOA STUDIED INVITRO', ISRAEL JOURNAL OF MEDICAL SCIENCES, 17 780-781 (1981) |
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1981 |
AITKEN RJ, RICHARDSON DW, 'ACTIVE IMMUNIZATION OF RATS WITH CUMULUS-FREE MOUSE OVA - INDUCTION OF INFERTILITY AND ANTIBODY-TITERS', JOURNAL OF EXPERIMENTAL ZOOLOGY, 216 149-158 (1981)
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1981 |
COOPER DW, AITKEN RJ, 'FAILURE TO DETECT ALTERED ROSETTE INHIBITION TITERS IN HUMAN-PREGNANCY SERUM', JOURNAL OF REPRODUCTION AND FERTILITY, 61 241-245 (1981)
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1981 |
AITKEN RJ, RUDAK EA, RICHARDSON DW, DOR J, DJAHANBAHKCH O, TEMPLETON AA, 'THE INFLUENCE OF ANTI-ZONA AND ANTI-SPERM ANTIBODIES ON SPERM-EGG INTERACTIONS', JOURNAL OF REPRODUCTION AND FERTILITY, 62 597-+ (1981)
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1981 |
AITKEN RJ, RICHARDSON DW, 'MEASUREMENT OF THE SPERM BINDING-CAPACITY OF THE MOUSE ZONA PELLUCIDA AND ITS USE IN THE ESTIMATION OF ANTI-ZONA ANTIBODY-TITERS', JOURNAL OF REPRODUCTION AND FERTILITY, 63 295-& (1981)
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1981 |
CANIVENC R, MAUGET C, BONNIN M, AITKEN RJ, 'DELAYED IMPLANTATION IN THE BEECH MARTEN (MARTES-FOINA)', JOURNAL OF ZOOLOGY, 193 325-332 (1981)
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1981 |
Aitken RJ, 'Aspects of delayed implantation in the roe deer (Capreolus capreolus)', Journal of Reproduction and Fertility Supplement, 83-95 (1981)
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1981 |
Aitken RJ, 'A review of gamete research, archives of andrology and reproduction', Nature, 345 (1981) |
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1981 |
AITKEN RJ, RICHARDSON DW, 'MECHANISM OF SPERM-BINDING INHIBITION BY ANTI-ZONA ANTISERA', GAMETE RESEARCH, 4 41-47 (1981)
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1980 |
DJAHANBAKHCH O, MCNEILLY AS, HOBSON BM, AITKEN RJ, 'APPLICATION OF RAPID LH RADIOIMMUNOASSAY FOR PREDICTION OF OVULATION', ARCHIVES OF ANDROLOGY, 5 19-19 (1980)
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1979 |
AITKEN RJ, 'CONTRACEPTIVE RESEARCH AND DEVELOPMENT', BRITISH MEDICAL BULLETIN, 35 199-+ (1979)
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1979 |
AITKEN RJ, 'TUBAL AND UTERINE SECRETIONS - POSSIBILITIES FOR CONTRACEPTIVE ATTACK', JOURNAL OF REPRODUCTION AND FERTILITY, 55 247-& (1979)
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1978 |
MAATHUIS JB, AITKEN RJ, 'CYCLIC VARIATION IN CONCENTRATIONS OF PROTEIN AND HEXOSE IN HUMAN UTERINE FLUSHINGS COLLECTED BY AN IMPROVED TECHNIQUE', JOURNAL OF REPRODUCTION AND FERTILITY, 52 289-295 (1978)
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1978 |
MAATHUIS JB, AITKEN RJ, 'PROTEIN PATTERNS OF HUMAN UTERINE FLUSHINGS COLLECTED AT VARIOUS STAGES OF MENSTRUAL-CYCLE', JOURNAL OF REPRODUCTION AND FERTILITY, 53 343-+ (1978)
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1978 |
Aitken RJ, 'The hormonal control of implantation.', Ciba Foundation symposium, 53-83 (1978)
The hormonal control of implantation in mammalian species with and without embryonic diapause is described. In a majority of species displaying the obligate form of diapause the c... [more]
The hormonal control of implantation in mammalian species with and without embryonic diapause is described. In a majority of species displaying the obligate form of diapause the corpora lutea appear to exhibit a low level of steroidogenic activity throughout diapause, full luteal activity being resumed just before the initiation of implantation. Fluctuations in the plasma levels of oestrogen and progesterone during diapause may serve to prime the uterus for implantation. In species exhibiting the facultative form of diapause, such as the rat and mouse, both progesterone and nidatory oestrogen are required for the induction of implantation. In species not displaying embryonic diapause implantation will take place in the presence of progesterone alone. In the light of these considerations the selection of animal models for drug-screening purposes and possible new approaches to contraception are discussed.
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1978 |
AITKEN RJ, MAATHUIS JB, 'EFFECT OF HUMAN UTERINE FLUSHINGS COLLECTED AT VARIOUS STAGES OF MENSTRUAL-CYCLE ON MOUSE BLASTOCYSTS INVITRO', JOURNAL OF REPRODUCTION AND FERTILITY, 53 137-140 (1978)
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1977 |
AITKEN RJ, CARTER J, 'BEHAVIORAL CONSEQUENCES OF VASECTOMY IN MOUSE', EXPERIENTIA, 33 1396-1397 (1977)
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1977 |
AITKEN RJ, 'PROTEIN-CONTENT OF MOUSE UTERINE FLUSHINGS DURING PSEUDOPREGNANCY', JOURNAL OF REPRODUCTION AND FERTILITY, 50 191-& (1977)
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1977 |
AITKEN RJ, 'INFLUENCE OF ACTINOMYCIN-D ON PROTEIN-COMPOSITION OF MOUSE UTERINE FLUSHINGS', JOURNAL OF REPRODUCTION AND FERTILITY, 50 193-195 (1977)
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1977 |
Aitken RJ, Harper MJK, 'New methods for the regulation of implantation', Contraception, 227-241 (1977)
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1977 |
Bonnin M, Canivenc R, Aitken RJ, 'Seasonal variations of plasma progesterone in the beech martin, Martes foina, a species which exhibits delayed implantation', C.R. Hebd Seanc. Acad Sci. Paris D, 1479-1482 (1977) |
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1977 |
AITKEN RJ, BOWMAN P, GAULD I, 'EFFECT OF SYNCHRONOUS AND ASYNCHRONOUS EGG TRANSFER ON FETAL WEIGHT IN MICE SELECTED FOR LARGE AND SMALL BODY SIZE', JOURNAL OF EMBRYOLOGY AND EXPERIMENTAL MORPHOLOGY, 37 59-64 (1977)
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1977 |
AITKEN RJ, 'CULTURE OF MOUSE BLASTOCYSTS IN PRESENCE OF UTERINE FLUSHINGS COLLECTED DURING NORMAL-PREGNANCY, DELAYED IMPLANTATION AND PRO-ESTRUS', JOURNAL OF EMBRYOLOGY AND EXPERIMENTAL MORPHOLOGY, 41 295-300 (1977)
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1977 |
AITKEN RJ, 'CHANGES IN PROTEIN-CONTENT OF MOUSE UTERINE FLUSHINGS DURING NORMAL-PREGNANCY AND DELAYED IMPLANTATION, AND AFTER OVARIECTOMY AND ESTRADIOL ADMINISTRATION', JOURNAL OF REPRODUCTION AND FERTILITY, 50 29-36 (1977)
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1976 |
AITKEN RJ, 'UTERINE SECRETION OF FRUCTOSE IN ROE DEER', JOURNAL OF REPRODUCTION AND FERTILITY, 46 439-440 (1976)
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1976 |
Snow HML, Aitken RJ, Ansell J, 'The role of the inner cell mass in controlling ovo-implantation in the mouse', Journal of Reproduction and Fertility, 403-404 (1976)
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1975 |
AITKEN RJ, 'ULTRASTRUCTURE OF BLASTOCYST AND ENDOMETRIUM OF ROE DEER (CAPREOLUS-CAPREOLUS) DURING DELAYED IMPLANTATION', JOURNAL OF ANATOMY, 119 369-384 (1975)
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1975 |
AITKEN RJ, 'CEMENTUM LAYERS AND TOOTH WEAR AS CRITERIA FOR AGING ROE DEER (CAPREOLUS-CAPREOLUS)', JOURNAL OF ZOOLOGY, 175 15-28 (1975)
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1975 |
Aitken RJ, 'Ultrastructure of the blastocyst and endometrium of the roe deer (Capreolus capreolus) during delayed implantation', Journal of Anatomy, 119 369-384 (1975)
Transmission electron microscopy of the trophoblast cells during diapause revealed an abundance of electron dense, membrane bound granular inclusions and a marked lack of cytoplas... [more]
Transmission electron microscopy of the trophoblast cells during diapause revealed an abundance of electron dense, membrane bound granular inclusions and a marked lack of cytoplasmic organelles. The cells also possessed a well developed covering of branched microvilli, numerous caveolae, micropinocytotic vesicles and a lamina of fine fibrillae. The progressive enlargement of the blastocyst during diapause was correlated with a decline in the height of the trophoblast cells and a reduction in the density of microvilli and caveolae associated with the outer membrane. The granular inclusions also declined in number and electron density during the delay phase, suggesting the progressive utilisation of energy reserves. Embryonic elongation was associated with the disappearance of the granular inclusions, a reduction in the amount of fibrillar material and a dramatic increase in the development of cytoplasmic organelles. During diapause, clear vesicles, apparently derived from the Golgi apparatus, gradually accumulated in the supranuclear region of each gland and non ciliated duct cell. Embryonic elongation was associated with the sudden release of these vesicles into the glandular lumen and thence into the uterine lumen. Numerous apical protrusions were also observed projecting from the luminal and ductal epithelia at this time, suggesting the formation of an apocrine secretion. Another type of secretion was produced during the early stages of placental attachment by the hypertrophied granular endoplasmic reticulum of the ductal epithelium.
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1974 |
AITKEN RJ, 'DELAYED IMPLANTATION IN ROE DEER (CAPREOLUS-CAPREOLUS)', JOURNAL OF REPRODUCTION AND FERTILITY, 225-+ (1974)
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1974 |
AITKEN RJ, 'SEX-CHROMATIN FORMATION IN BLASTOCYST OF ROE DEER (CAPREOLUS-CAPREOLUS) DURING DELAYED IMPLANTATION', JOURNAL OF REPRODUCTION AND FERTILITY, 40 235-239 (1974)
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1974 |
AITKEN RJ, 'CALCIUM AND ZINC IN ENDOMETRIUM AND UTERINE FLUSHINGS OF ROE DEER (CAPREOLUS-CAPREOLUS) DURING DELAYED IMPLANTATION', JOURNAL OF REPRODUCTION AND FERTILITY, 40 333-340 (1974)
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1973 |
AITKEN RJ, BURTON J, HAWKINS J, KERRWILS R, SHORT RV, STEVEN DH, 'HISTOLOGICAL AND ULTRASTRUCTURAL CHANGES IN BLASTOCYST AND REPRODUCTIVE TRACT OF ROE DEER, CAPREOLUS-CAPREOLUS, DURING DELAYED IMPLANTATION', JOURNAL OF REPRODUCTION AND FERTILITY, 34 481-& (1973)
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1971 |
Aitken RJ, Burton JHW, Steven DH, 'Ultrastructural changes in the uterine glands of the roe deer during delayed implantation', Journal of Physiology, 13-15 (1971) |
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