
Professor Peter Lewis
Professor
School of Environmental and Life Sciences (Biological Sciences)
- Email:peter.lewis@newcastle.edu.au
- Phone:(02) 4921 5701
Career Summary
Biography
My research career has focused on the gram positive bacterium Bacillus subtilis. During my PhD I purified and characterised the replication terminator protein (RTP) and its DNA binding sites (Lewis et al., J. Bacteriol 171, 3564-3567 (1989); Lewis et al., J. Mol. Biol. 214, 73-84 (1990)). These sites are regions of DNA where oppositely moving replication forks meet and newly replicated chromosomes are resolved prior to segregation. During my post-doctoral research I was responsible for the development of cell biological techniques and vectors for visualising gene expression and protein localisation in live bacterial cells (Lewis et al., PNAS 91, 3849-3853 (1994); Lewis et al., Mol Microbiol 13, 655-662 (1994); Lewis and Errington, Microbiology 142, 733-740 (1996); Lewis and Marston, Gene 227, 101-109 (1999); Feucht and Lewis, Gene 264, 289-297 (2001)).
These techniques have been widely adopted by research groups involved in microbial cell biology, and the plasmid vectors have now been distributed directly to well over 100 laboratories world-wide, although they are now also distributed by the Bacillus Genetic Stock Centre. These techniques were instrumental in determining the establishment of compartment specificity of developmentally regulated s-factors during sporulation in B. subtilis (Lewis et al., PNAS 91, 3849-3853 (1994)). I also showed that prespore specific accumulation of the transcription regulator SpoIIAA was responsible for initiation of compartment-specific gene expression during development, that this accumulation was probably due to prespore-specific activity of the phosphatase SpoIIE, and that a programme of proteolysis was initiated following this activation event (Lewis et al., Genes Cell 1, 881-894 (1996); Lewis et al., J. Bacteriol 180, 3276-3284 (1998)). I was also involved in work that showed that the highly conserved SpoIIIE (FtsK) protein is a DNA translocase that moves DNA through a division septum (Wu et al., Genes Dev 9, 1316-1326 (1995)). This was the first example of such a phenomenon, and was a very significant finding as previously it was assumed that DNA was segregated into daughter cells/different compartments prior to division septum formation. Finally, I have shown that transcription and translation are spatially separated within bacteria (Lewis et al., EMBO J 19, 710-718 (2000)). This was an unexpected result as the 2 processes were thought to be very tightly coupled in bacteria. Furthermore, transcription becomes concentrated into a sub-fraction of the bacterial nucleoid at higher growth rates. These transcription foci have been shown to be the sites of rRNA synthesis, and my laboratory is now focussing on characterising transcription complexes and their structure in detail.
Research Expertise
My research career has focused on the gram positive bacterium Bacillus subtilis. During my PhD I purified and characterised the replication terminator protein (RTP) and its DNA binding sites (Lewis et al., J. Bacteriol 171, 3564-3567 (1989); Lewis et al., J. Mol. Biol. 214, 73-84 (1990)). These sites are regions of DNA where oppositely moving replication forks meet and newly replicated chromosomes are resolved prior to segregation. During my post-doctoral research I was responsible for the development of cell biological techniques and vectors for visualising gene expression and protein localisation in live bacterial cells (Lewis et al., PNAS 91, 3849-3853 (1994); Lewis et al., Mol Microbiol 13, 655-662 (1994); Lewis and Errington, Microbiology 142, 733-740 (1996); Lewis and Marston, Gene 227, 101-109 (1999); Feucht and Lewis, Gene 264, 289-297 (2001)). These techniques have been widely adopted by research groups involved in microbial cell biology, and the plasmid vectors have now been distributed directly to well over 100 laboratories world-wide, although they are now also distributed by the Bacillus Genetic Stock Centre. These techniques were instrumental in determining the establishment of compartment specificity of developmentally regulated s-factors during sporulation in B. subtilis (Lewis et al., PNAS 91, 3849-3853 (1994)). I also showed that prespore specific accumulation of the transcription regulator SpoIIAA was responsible for initiation of compartment-specific gene expression during development, that this accumulation was probably due to prespore-specific activity of the phosphatase SpoIIE, and that a programme of proteolysis was initiated following this activation event (Lewis et al., Genes Cell 1, 881-894 (1996); Lewis et al., J. Bacteriol 180, 3276-3284 (1998)). I was also involved in work that showed that the highly conserved SpoIIIE (FtsK) protein is a DNA translocase that moves DNA through a division septum (Wu et al., Genes Dev 9, 1316-1326 (1995)). This was the first example of such a phenomenon, and was a very significant finding as previously it was assumed that DNA was segregated into daughter cells/different compartments prior to division septum formation. Finally, I have shown that transcription and translation are spatially separated within bacteria (Lewis et al., EMBO J 19, 710-718 (2000)). This was an unexpected result as the 2 processes were thought to be very tightly coupled in bacteria. Furthermore, transcription becomes concentrated into a sub-fraction of the bacterial nucleoid at higher growth rates. These transcription foci have been shown to be the sites of rRNA synthesis, and my laboratory is now focussing on characterising transcription complexes and their structure in detail.
Teaching Expertise
Molecular biology, particularly bacterial. Microbiology.
Administrative Expertise
No expertise.
Qualifications
- Doctor of Philosophy, University of Sydney
- Bachelor of Science, University of Sheffield - UK
Keywords
- Biochemistry
- Microscopy
- Molecular Biology
- Molecular Microbiology
- Transcription regulation
Professional Experience
UON Appointment
Title | Organisation / Department |
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Professor | University of Newcastle School of Environmental and Life Sciences Australia |
Professor | University of Newcastle School of Environmental and Life Sciences Australia |
Academic appointment
Dates | Title | Organisation / Department |
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1/7/2005 - | Membership - National Convener Molecular Microbiology Special Interest Group | Australian Society for Microbiology Australia |
1/7/2000 - | Senior Lecturer | University of Newcastle School of Environmental and Life Sciences Australia |
Membership
Dates | Title | Organisation / Department |
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1/1/2006 - | OzReader | ARC Biological Sciences Committee Australia |
1/1/2004 - | Member of EU 6th Framework Consortium for A$20M EU 6th Framework BaSysBio Project | European Union (EU) Framework Collaboration Australia |
Professional appointment
Dates | Title | Organisation / Department |
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21/7/2008 - 21/8/2012 | NHMRC Grant Review Panel | National Health and Medical Research Council Australia |
Awards
Research Award
Year | Award |
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2004 |
International Short-Term Fellowship Australian Academy of Sciences |
Invitations
Participant
Year | Title / Rationale |
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2007 |
Invited speaker Organisation: Conference Description: Invited to speak at SGM 162nd Meeting-Edinburgh International Conference Centre |
2007 |
Reviewer Organisation: Various Publishers in the field of Environmental and Life Science Description: Have been invited to write reviews for top journals in field (Molecular Microbiology, Microbiology and international Review of Cytology) and 2 book chapters in the last 3 years. Work is also now appearing in Microbiology text books. |
Teaching
Code | Course | Role | Duration |
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BIOL3090 |
Molecular Biology Faculty of Science and IT, University of Newcastle |
Course coordinator/lecturer | 21/2/2002 - 21/8/2016 |
BTEC3250 |
Biotech Placement Faculty of Science and IT, University of Newcastle |
Course coordinator (2012)/supervisor | 21/7/2001 - 21/8/2016 |
BIOL3001 |
Advanced Lab Skills in Biology Faculty of Science and IT, University of Newcastle |
Course coordinator/Lecturer | 21/2/2007 - 21/8/2016 |
BIOL2002 |
Lab Skills in Biology Faculty of Science and Information Technology, University of Newcastle |
Lecturer | 21/7/2008 - 31/12/2012 |
BIOL3100 |
Microbiology Faculty of Science and Information Technology, University of Newcastle |
Course coordinator/lecturer | 21/2/2016 - 21/8/2016 |
BIOL1003 |
Biology Professional Skills 1 Faculty of Science and Information Technology, University of Newcastle |
Lecturer | 21/7/2011 - 21/8/2015 |
BIOL2090 |
Microbial Biology Faculty of Science and Information Technology, University of Newcastle |
Lecturer/cours coordinator (up to 2010) | 21/7/2001 - 21/7/2016 |
Publications
For publications that are currently unpublished or in-press, details are shown in italics.
Chapter (6 outputs)
Year | Citation | Altmetrics | Link | ||
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2012 |
Doherty GP, Mettrick KA, Grainge IR, Lewis PJ, 'Imaging fluorescent protein fusions in live bacteria', Methods in Microbiology, Academic Press, Kidlington, Ox 107-126 (2012) [B1]
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2012 | Lewis PJ, Yang X, 'The organisation of transcription and translation', Bacillus: Cellular and Molecular Biology, Caister Academic Press, Norfolk, UK 123-150 (2012) [B1] | ||||
2008 | Lewis PJ, 'Subcellular organisation in bacteria', Bacterial Physiology: A Molecular Approach, Springer, Berlin 1-42 (2008) [B1] | ||||
2007 | Lewis PJ, 'The organization of transcription and translation', Bacillus: Cellular and Molecular Biology, Caister Academic Press, Norfolk, United Kingdom 135-166 (2007) [B1] | ||||
Show 3 more chapters |
Journal article (79 outputs)
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2020 |
Baker M, 'Sialylation of Asparagine 612 inhibits Aconitase activity during mouse sperm capacitation; A possible mechanism for the switch from oxidative phosphorylation to glycolysis', BioRxiv, (2020) [C1]
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2020 |
Cossar PJ, Lewis PJ, McCluskey A, 'Protein-protein interactions as antibiotic targets: A medicinal chemistry perspective', Medicinal Research Reviews, 40 469-494 (2020) [C1]
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2020 |
Newing TP, Oakley AJ, Miller M, Dawson CJ, Brown SHJ, Bouwer JC, et al., 'Molecular basis for RNA polymerase-dependent transcription complex recycling by the helicase-like motor protein HelD', Nature Communications, 11 (2020) [C1] © 2020, The Author(s). In bacteria, transcription complexes stalled on DNA represent a major source of roadblocks for the DNA replication machinery that must be removed in order t... [more] © 2020, The Author(s). In bacteria, transcription complexes stalled on DNA represent a major source of roadblocks for the DNA replication machinery that must be removed in order to prevent damaging collisions. Gram-positive bacteria contain a transcription factor HelD that is able to remove and recycle stalled complexes, but it was not known how it performed this function. Here, using single particle cryo-electron microscopy, we have determined the structures of Bacillus subtilis RNA polymerase (RNAP) elongation and HelD complexes, enabling analysis of the conformational changes that occur in RNAP driven by HelD interaction. HelD has a 2-armed structure which penetrates deep into the primary and secondary channels of RNA polymerase. One arm removes nucleic acids from the active site, and the other induces a large conformational change in the primary channel leading to removal and recycling of the stalled polymerase, representing a novel mechanism for recycling transcription complexes in bacteria.
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2017 |
de Jong L, de Koning EA, Roseboom W, Buncherd H, Wanner MJ, Dapic I, et al., 'In-Culture Cross-Linking of Bacterial Cells Reveals Large-Scale Dynamic Protein-Protein Interactions at the Peptide Level', JOURNAL OF PROTEOME RESEARCH, 16 2457-2471 (2017) [C1]
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2017 |
Yang X, Luo MJ, Yeung ACM, Lewis PJ, Chan PKS, Ip M, Ma C, 'First-In-Class Inhibitor of Ribosomal RNA Synthesis with Antimicrobial Activity against Staphylococcus aureus', Biochemistry, 56 5049-5052 (2017) [C1] © 2017 American Chemical Society. We report the discovery of the first bacterial ribosomal RNA (rRNA) synthesis inhibitor that has specific antimicrobial activity against methicil... [more] © 2017 American Chemical Society. We report the discovery of the first bacterial ribosomal RNA (rRNA) synthesis inhibitor that has specific antimicrobial activity against methicillin-resistant Staphylococcus aureus (MRSA). A pharmacophore model was constructed on the basis of the protein-protein interaction between essential bacterial rRNA transcription factors NusB and NusE and employed for an in silico screen to identify potential leads. One compound, (E)-2-{[(3-ethynylphenyl)imino]methyl}-4-nitrophenol (MC4), demonstrated antimicrobial activity against a panel of S. aureus strains, including MRSA, without significant toxicity to mammalian cells. MC4 resulted in a decrease in the rRNA level in bacteria, and the target specificity of MC4 was confirmed at the molecular level. Results obtained from this work validated the bacterial rRNA transcription machinery as a novel antimicrobial target. This approach may be extended to other factors in rRNA transcription, and MC4 could be applied as a chemical probe to dissect the relationship among MRSA infection, MRSA growth rate, and rRNA synthesis, in addition to its therapeutic potential.
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2017 |
Bi Y, Liu X-X, Zhang H-Y, Yang X, Liu Z-Y, Lu J, et al., 'Synthesis and Antibacterial Evaluation of Novel 3-Substituted Ocotillol-Type Derivatives as Leads', MOLECULES, 22 (2017) [C1]
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2017 |
Cossar PJ, Ma C, Gordon CP, Ambrus JI, Lewis PJ, McCluskey A, 'Identification and validation of small molecule modulators of the NusB-NusE interaction', BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 27 162-167 (2017) [C1]
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2017 |
Wenholz DS, Zeng M, Ma C, Mielczarek M, Yang X, Bhadbhade M, et al., 'Small molecule inhibitors of bacterial transcription complex formation', Bioorganic and Medicinal Chemistry Letters, 27 4302-4308 (2017) [C1]
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2017 |
Cossar PJ, Abdel-Hamid MK, Ma C, Sakoff JA, Trinh TN, Gordon CP, et al., 'Small-Molecule Inhibitors of the NusB-NusE Protein-Protein Interaction with Antibiotic Activity', ACS OMEGA, 2 3839-3857 (2017) [C1]
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2016 |
Thach O, Mielczarek M, Ma C, Kutty SK, Yang X, Black DS, et al., 'From indole to pyrrole, furan, thiophene and pyridine: Search for novel small molecule inhibitors of bacterial transcription initiation complex formation', BIOORGANIC & MEDICINAL CHEMISTRY, 24 1171-1182 (2016) [C1]
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2016 |
Ma C, Yang X, Lewis PJ, 'Bacterial Transcription as a Target for Antibacterial Drug Development', MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS, 80 139-160 (2016) [C1]
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2016 |
Keller AN, Xin Y, Boer S, Reinhardt J, Baker R, Arciszewska LK, et al., 'Activation of Xer-recombination at dif: structural basis of the FtsK gamma-XerD interaction', SCIENTIFIC REPORTS, 6 (2016) [C1]
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2016 |
Ma C, Yang X, Lewis PJ, 'Bacterial Transcription Inhibitor of RNA Polymerase Holoenzyme Formation by Structure-Based Drug Design: From in Silico Screening to Validation', ACS Infectious Diseases, 2 39-46 (2016) [C1] © 2015 American Chemical Society. Bacterial transcription is a proven target for antibacterial research. However, most of the known inhibitors targeting transcription are from nat... [more] © 2015 American Chemical Society. Bacterial transcription is a proven target for antibacterial research. However, most of the known inhibitors targeting transcription are from natural extracts or are hits from screens where the binding site remains unidentified. Using an RNA polymerase holoenzyme homology structure from the model Gram-positive organism Bacillus subtilis, we created a pharmacophore model and used it for in silico screening of a publicly available library for compounds able to inhibit holoenzyme formation. The hits demonstrated specific affinity to bacterial RNA polymerase and excellent activity using in vitro assays and showed no binding to the equivalent structure from human RNA polymerase II. The target specificity in live cells and antibacterial activity was demonstrated in microscopy and growth inhibition experiments. This is the first example of targeted inhibitor development for a bacterial RNA polymerase, outlining a complete discovery process from virtual screening to biochemical validation. This approach could serve as an appropriate platform for the future identification of inhibitors of bacterial transcription.
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2015 |
Mielczarek M, Thomas RV, Ma C, Kandemir H, Yang X, Bhadbhade M, et al., 'Synthesis and biological activity of novel mono-indole and mono-benzofuran inhibitors of bacterial transcription initiation complex formation', Bioorganic and Medicinal Chemistry, (2015) [C1] Our ongoing research focused on targeting transcription initiation in bacteria has resulted in synthesis of several classes of mono-indole and mono-benzofuran inhibitors that targ... [more] Our ongoing research focused on targeting transcription initiation in bacteria has resulted in synthesis of several classes of mono-indole and mono-benzofuran inhibitors that targeted the essential protein-protein interaction between RNA polymerase core and s 70/s A factors in bacteria. In this study, the reaction of indole-2-, indole-3-, indole-7- and benzofuran-2-glyoxyloyl chlorides with amines and hydrazines afforded a variety of glyoxyloylamides and glyoxyloylhydrazides. Similarly, condensation of 2- and 7-trichloroacetylindoles with amines and hydrazines delivered amides and hydrazides. The novel molecules were found to inhibit the RNA polymerase-s 70/s A interaction as measured by ELISA, and also inhibited the growth of both Gram-positive and Gram-negative bacteria in culture. Structure-activity relationship (SAR) studies of the mono-indole and mono-benzofuran inhibitors suggested that the hydrophilic-hydrophobic balance is an important determinant of biological activity.
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2015 |
Yin Z, Whittell LR, Wang Y, Jergic S, Ma C, Lewis PJ, et al., 'Bacterial Sliding Clamp Inhibitors that Mimic the Sequential Binding Mechanism of Endogenous Linear Motifs.', J Med Chem, 58 4693-4702 (2015) [C1]
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2015 |
Bi Y, Yang X, Zhang T, Liu Z, Zhang X, Lu J, et al., 'Design, synthesis, nitric oxide release and antibacterial evaluation of novel nitrated ocotillol-type derivatives', European Journal of Medicinal Chemistry, 101 71-80 (2015) [C1] © 2015 Elsevier Masson SAS. Abstract Nitric oxide (NO) and its auto-oxidation products are known to disrupt normal bacterial function and NO releasing molecules have the potential... [more] © 2015 Elsevier Masson SAS. Abstract Nitric oxide (NO) and its auto-oxidation products are known to disrupt normal bacterial function and NO releasing molecules have the potential to be developed as antibacterial leads in drug discovery. We have designed and synthesized a series of novel nitrated compounds by combining NO releasing groups with ocotillol-type triterpenoids, which have previously demonstrated activity only against Gram-positive bacteria. The in vitro NO release capacity and antibacterial activity were sequentially evaluated and the data showed that most of the synthesized compounds could release nitric oxide. Compound 16a, 17a and 17c, with nitrated aliphatic esters at C-3 position, displayed higher NO release than other analogues, correlating to their good antibacterial activity, in which 17c demonstrated broad-spectrum activity against both Gram positive and -negative bacteria, as well as excellent synergism at sub-minimum inhibitory concentration when using with kanamycin and chloramphenicol. Furthermore, the epifluorescent microscopic study indicated that the ocotillol-type triterpenoid core may induce NO release on the bacterial membrane. Our results demonstrate that nitrated substitutions at C-3 of ocotillol-type derivatives could provide an approach to expand their antibacterial spectrum, and that ocotillol-type triterpenoids may also be developed as appropriate carriers for NO donors in antibacterial agent discovery with low cytotoxicity.
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2015 |
Ma C, Mobli M, Yang X, Keller AN, King GF, Lewis PJ, 'RNA polymerase-induced remodelling of NusA produces a pause enhancement complex.', Nucleic acids research, 43 2829-2840 (2015) [C1]
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2015 |
Yang X, Ma C, Lewis PJ, 'Identification of inhibitors of bacterial RNA polymerase', Methods, 86 45-50 (2015) [C1] © 2015. Very few clinically available antibiotics target bacterial RNA polymerase (RNAP) suggesting it is an underutilized target. The advent of detailed structural information of... [more] © 2015. Very few clinically available antibiotics target bacterial RNA polymerase (RNAP) suggesting it is an underutilized target. The advent of detailed structural information of RNAP holoenzyme (HE) has allowed the design and in silico screening of novel transcription inhibitors. Here, we describe our approach for the design and testing of small molecule transcription inhibitors that work by preventing the interaction between the essential transcription initiation factor s and RNAP. With the appropriate structural information this approach can be easily modified to other essential protein-protein interactions.
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2015 |
Bi Y, Ma C, Zhou Z, Zhang T, Zhang H, Zhang X, et al., 'Synthesis and antibacterial evaluation of novel hydrophilic ocotillol-type triterpenoid derivatives from 20(S)-protopanaxadiol', Records of Natural Products, 9 356-368 (2015) [C1] © 2015 ACG Publications. All rights reserved. Triterpenoid saponins are involved in plant defense systems to inhibit bacterial invasion. A new series of hydrophilic ocotillol-type... [more] © 2015 ACG Publications. All rights reserved. Triterpenoid saponins are involved in plant defense systems to inhibit bacterial invasion. A new series of hydrophilic ocotillol-type triterpenoid derivatives 5-26 have been synthesized with antibacterial activity against Gram-positive bacteria, including a community associated methicillin-resistant Staphylococcus aureus (CA-MRSA; strain USA300). From this series, compounds 6 and 15 were found to be the most active, both with MIC values of 2 µg/mL against B. subtilis 168 and 8 µg/mL against S. aureus USA300, respectively. Furthermore, subsequent assays showed that compounds 6 and 15 displayed strong synergistic effects at sub-MIC levels against both S. aureus USA300 and B. subtilis 168 when combined with two commercial antibiotics, kanamycin and chloramphenicol. Preliminary structure-activity relationship studies were also performed.
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2014 |
Mielczarek M, Devakaram RV, Ma C, Yang X, Kandemir H, Purwono B, et al., 'Synthesis and biological activity of novel bis-indole inhibitors of bacterial transcription initiation complex formation', Organic and Biomolecular Chemistry, 12 2882-2894 (2014) [C1] The increasing resistance of bacteria against clinically approved antibiotics is resulting in an alarming decrease in therapeutic options for today's clinicians. We have targ... [more] The increasing resistance of bacteria against clinically approved antibiotics is resulting in an alarming decrease in therapeutic options for today's clinicians. We have targeted the essential interaction between bacterial RNA polymerase and s70/sA for the development of lead molecules exhibiting a novel mechanism of antibacterial activity. Several classes of structurally related bis-indole inhibitors of bacterial transcription initiation complex formation were synthesized and their antimicrobial activities were evaluated. Condensation of indole-7- and indole-2-carbohydrazides with 7- and 2-trichloroacetylindoles or indole-7- and indole-2-glyoxyloyl chlorides resulted in the successful synthesis of 7,7'-, 2,2'-, 2,7'- and 3,2'-linked bis-indole derivatives with -CO-NH-NH-CO- and -CO-CO-NH-NH-CO- linkers. Indole-7-glyoxyloyl chlorides were reacted with hydrazine hydrate in different ratios to afford respective -CO-CO-NH-NH-CO-CO- bis-indole or hydrazide derivatives. The resulting compounds were found to be active against the ß'-CH- s70/sA2.2 interaction in ELISA assays and inhibited the growth of both Gram-positive and Gram-negative bacteria. Structure-activity relationship (SAR) studies were performed in order to identify the structural features of the synthesized inhibitors required for biological activity. This journal is © the Partner Organisations 2014.
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2014 |
Keller AN, Yang X, Wiedermannová J, Delumeau O, Krásný L, Lewis PJ, 'e, a new subunit of RNA polymerase found in gram-positive bacteria.', J Bacteriol, 196 3622-3632 (2014) [C1]
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2014 |
Withers R, Doherty GP, Jordan M, Yang X, Dixon NE, Lewis PJ, 'AtfA, a new factor in global regulation of transcription in Acinetobacter spp', Molecular Microbiology, 93 1130-1143 (2014) [C1] © 2014 John Wiley & Sons Ltd. Acinetobacter species are widely distributed bacteria in the environment, and have recently gained notoriety as opportunistic nosocomial pathog... [more] © 2014 John Wiley & Sons Ltd. Acinetobacter species are widely distributed bacteria in the environment, and have recently gained notoriety as opportunistic nosocomial pathogens. Here we characterize a novel RNA polymerase-interacting protein named acidic transcription factor A, AtfA. It is small and highly acidic, and is widely distributed throughout the ¿ proteobacteria, including other significant pathogens in the genera Moraxella, Pseudomonas, Legionella and Vibrio. In the model species A. baylyiADP1, deletion of atfA significantly affects expression of over 500 genes, resulting in a large cell phenotype, reduced cell fitness, impaired biofilm formation and twitching motility, and increased sensitivity to antibiotics. Deletion of atfA also causes dramatically enhanced sensitivity to ethanol, which is an important growth promoter and virulence factor in Acinetobacter spp. The results suggest that auxiliary factors of RNA polymerase with important biological roles remain to be discovered.
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2014 |
Buczkowicz P, Hoeman C, Rakopoulos P, Pajovic S, Letourneau L, Dzamba M, et al., 'Genomic analysis of diffuse intrinsic pontine gliomas identifies three molecular subgroups and recurrent activating ACVR1 mutations', Nature Genetics, 46 451-456 (2014) Diffuse intrinsic pontine glioma (DIPG) is a fatal brain cancer that arises in the brainstem of children, with no effective treatment and near 100% fatality. The failure of most t... [more] Diffuse intrinsic pontine glioma (DIPG) is a fatal brain cancer that arises in the brainstem of children, with no effective treatment and near 100% fatality. The failure of most therapies can be attributed to the delicate location of these tumors and to the selection of therapies on the basis of assumptions that DIPGs are molecularly similar to adult disease. Recent studies have unraveled the unique genetic makeup of this brain cancer, with nearly 80% found to harbor a p.Lys27Met histone H3.3 or p.Lys27Met histone H3.1 alteration. However, DIPGs are still thought of as one disease, with limited understanding of the genetic drivers of these tumors. To understand what drives DIPGs, we integrated whole-genome sequencing with methylation, expression and copy number profiling, discovering that DIPGs comprise three molecularly distinct subgroups (H3-K27M, silent and MYCN) and uncovering a new recurrent activating mutation affecting the activin receptor gene ACVR1 in 20% of DIPGs. Mutations in ACVR1 were constitutively activating, leading to SMAD phosphorylation and increased expression of the downstream activin signaling targets ID1 and ID2. Our results highlight distinct molecular subgroups and novel therapeutic targets for this incurable pediatric cancer. © 2014 Nature America, Inc. All rights reserved.
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2014 |
Yang X, Ma C, Lewis P, 'A vector system that allows simple generation of mutant Escherichia coli RNA polymerase', PLASMID, 75 37-41 (2014) [C1]
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2014 |
Kandemir H, Kutty SK, Black DS, Kumar N, Ma C, Lewis PJ, Griffith R, 'Synthesis and biological evaluation of 2,5-di(7-indolyl)-1,3,4-oxadiazoles, and 2- and 7-indolyl 2-(1,3,4-thiadiazolyl)ketones as antimicrobials', Bioorganic and Medicinal Chemistry, (2014) [C1] A range of novel hydrazine bridged bis-indoles was prepared from readily available indole-7-glyoxyloylchlorides and 7-trichloroacetylindoles and underwent cyclodehydration to prod... [more] A range of novel hydrazine bridged bis-indoles was prepared from readily available indole-7-glyoxyloylchlorides and 7-trichloroacetylindoles and underwent cyclodehydration to produce 2,5-di(7-indolyl)-1,3,4-oxadiazoles and a 2,2'-bi-1,3,4-oxadiazolyl with phosphoryl chloride in ethyl acetate. This efficient protocol was subsequently used for the synthesis of 2- and 7-indolyl 2-(1,3,4-thiadiazolyl)ketones from related indolyl-hydrazine carbothioamides. The synthesised bis-indoles were evaluated for their antimicrobial properties, particularly the inhibition of protein-protein complex formation between RNA polymerase and s factor and their bactericidal effect on Gram positive Bacillus subtilis and Gram negative Escherichia coli. © 2014 Elsevier Ltd. All rights reserved.
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2014 |
Wiedermannová J, Sudzinová P, Koval T, Rabatinová A, anderova H, Ramaniuk O, et al., 'Characterization of HelD, an interacting partner of RNA polymerase from Bacillus subtilis.', Nucleic Acids Res, 42 5151-5163 (2014)
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2014 |
Bi Y, Ma C, Zhang H, Zhou Z, Yang J, Zhang Z, et al., 'Novel 3-Substituted Ocotillol-Type Triterpenoid Derivatives as Antibacterial Candidates', CHEMICAL BIOLOGY & DRUG DESIGN, 84 489-496 (2014) [C1]
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2013 |
Zhou Z, Ma C, Zhang H, Bi Y, Chen X, Tian H, et al., 'Synthesis and biological evaluation of novel ocotillol-type triterpenoid derivatives as antibacterial agents', European Journal of Medicinal Chemistry, 68 444-453 (2013) [C1]
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2013 |
Ma C, Yang X, Kandemir H, Mielczarek M, Johnston EB, Griffith R, et al., 'Inhibitors of Bacterial Transcription Initiation Complex Formation', ACS Chemical Biology, 8 1972-1980 (2013) [C1]
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2013 |
Brzoska AJ, Hassan KA, de Leon EJ, Paulsen IT, Lewis PJ, 'Single-Step Selection of Drug Resistant Acinetobacter baylyi ADP1 Mutants Reveals a Functional Redundancy in the Recruitment of Multidrug Efflux Systems', PLOS ONE, 8 (2013) [C1]
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2012 |
Buescher JM, Liebermeister W, Jules M, Uhr M, Muntel J, Botella E, et al., 'Global network reorganization during dynamic adaptations of Bacillus subtilis metabolism', Science, 335 1099-1103 (2012) [C1]
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2012 |
Nicolas P, Mader U, Dervyn E, Rochat T, Leduc A, Pigeonneau N, et al., 'Condition-dependent transcriptome reveals high-level regulatory architecture in Bacillus subtilis', Science, 335 1103-1106 (2012) [C1]
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2012 |
Buncherd H, Nessen MA, Nouse N, Stelder SK, Roseboom W, Dekker HL, et al., 'Selective enrichment and identification of cross-linked peptides to study 3-D structures of protein complexes by mass spectrometry', Journal of Proteomics, 75 2205-2215 (2012) [C1]
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2011 |
Liew ATF, Theis T, Jensen SO, Garcia-Lara J, Foster SJ, Firth N, et al., 'A simple plasmid-based system that allows rapid generation of tightly controlled gene expression in Staphylococcus aureus', Microbiology, 157 666-676 (2011) [C1]
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2010 |
Yang X, Lewis PJ, 'The interaction between RNA polymerase and the elongation factor NusA', RNA Biology, 7 272-275 (2010) [C1]
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2010 |
Yang X, Lewis PJ, 'The interaction between bacterail transcription factors and RNA polymerase during the transition from initiation to elongation', Transcription, 1 1-4 (2010) [C1]
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2010 |
Doherty GP, Bailey KL, Lewis PJ, 'Stage-specific fluorescence intensity of GFP and mCherry during sporulation in Bacillus Subtilis', BMC Research Notes, 3 1-8 (2010) [C1]
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2010 |
Robinson A, Brzoska AJ, Turner KM, Withers RW, Harry EJ, Lewis PJ, Dixon NE, 'Essential biological processes of an emerging pathogen: DNA replication, transcription, and cell division in Acinetobacter spp', Microbiology and Molecular Biology Reviews, 74 273-297 (2010) [C1]
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2010 |
Botella E, Fogg M, Jules M, Piersma S, Doherty GP, Hansen A, et al., 'pBaSysBioII: An integrative plasmid generating gfp transcriptional fusions for high-throughput analysis of gene expression in Bacillus subtilis', Microbiology, 156 1600-1608 (2010) [C1]
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2010 |
Doherty GP, Fogg MJ, Wilkinson AJ, Lewis PJ, 'Small subunits of RNA polymerase: Localization, levels and implications for core enzyme composition', Microbiology, 16 3532-3543 (2010) [C1]
|
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2009 |
Yang X, Molimau S, Doherty GP, Johnston EB, Marles-Wright J, Rothnagel R, et al., 'The structure of bacterial RNA polymerase in complex with the essential transcription elongation factor NusA', EMBO Reports, 10 997-1002 (2009) [C1]
|
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2009 |
Johnston EB, Lewis PJ, Griffith R, 'The interaction of Bacillus subtilis sigma(A) with RNA polymerase', Protein Science, 18 2287-2297 (2009) [C1]
|
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2008 |
Yang X, Lewis PJ, 'Overproduction and purification of recombinant Bacillus subtilis RNA polymerase', Protein Expression and Purification, 59 86-93 (2008) [C1]
|
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2008 |
Meredith DH, Plank MW, Lewis PJ, 'Different patterns of integral membrane protein localization during cell division in Bacillus subtilis', Microbiology, 154 64-71 (2008) [C1]
|
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2008 |
Lewis PJ, Doherty GP, Clarke J, 'Transcription factor dynamics', Microbiology, 154 1837-1844 (2008) [C1]
|
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2008 |
Marles-Wright J, Grant T, Delumeau O, Van Duinen G, Firbank SJ, Lewis PJ, et al., 'Molecular architecture of the 'stressosome,' a signal integration and transduction hub', Science, 322 92-96 (2008) [C1]
|
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2008 |
Yang X, Doherty GP, Lewis PJ, 'Tandem affinity purification vectors for use in gram positive bacteria', Plasmid, 59 54-62 (2008) [C1]
|
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2006 |
Doherty GP, Meredith DH, Lewis PJ, 'Subcellular partitioning of transcription factors in Bacillus subtilis', Journal of Bacteriology, 188 4101-4110 (2006) [C1]
|
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2005 |
Davies KM, Dedman AJ, van Horck S, Lewis PJ, 'The NusA:RNA polymerase ratio is increased at sites of rRNA synthesis in Bacillus subtilis.', Mol Microbiol, 57 366-379 (2005)
|
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2005 |
MacDougall IJA, Lewis PJ, Griffith R, 'Homology modelling of RNA polymerase and associated transcription factors from Bacillus subtilis', Journal of Molecular Graphics & Modelling, 23 297-303 (2005) [C1]
|
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2005 |
Davies KM, Dedman AJ, Van Horck SH, Lewis PJ, 'The NusA : RNA polymerase ratio is increased at sites of rRNA synthesis in Bacillus subtilis', Molecular Microbiology, 57 366-379 (2005) [C1]
|
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2004 |
Migocki MD, Lewis PJ, Wake RG, Harry EJ, 'The midcell replication factory in Bacillus subtilis is highly mobile: implications for coordinating chromosome replication with other cell cycle events', Molecular Microbiology, 54 452-463 (2004) [C1]
|
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2004 |
Lewis PJ, 'Bacterial subcellular architecture: recent advances and future prospects', Molecular Microbiology, 54 1135-1150 (2004) [C1]
|
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2004 |
Johnson AS, Van Horck SH, Lewis PJ, 'Dynamic localization of membrance proteins in Bacillus subtilis', Microbiology, 150 2815-2824 (2004) [C1]
|
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2003 |
Davies KM, Lewis PJ, 'Localization of rRNA Synthesis in Bacillus subtilis: Characterization of Loci Involved in Transcription Focus Formation', Journal of Bacteriology, 185 2346-2353 (2003) [C1]
|
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2003 |
Harry EJ, Lewis PJ, 'Early targeting of Min proteins to the cell poles in germinated spores of Bacillus subtilis: evidence for division apparatus-independent recruitment of Min proteins to the division site', Molecular Microbiology, 47 37-48 (2003) [C1]
|
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2001 |
Lewis PJ, 'Bacterial chromosome segregation', Microbiology, 147 519-526 (2001) [C1]
|
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2001 |
Feucht A, Lewis PJ, 'Improved plasmid vectors for the production of multiple fluorescent protein fusions in Bacillus subtilis', Gene, 264 289-297 (2001) [C1]
|
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2000 |
Meijer WJJ, Lewis PJ, Errington J, Salas M, 'Dynamic relocalization of phage f29 DNA during replication and the role of the viral protein p16.7', EMBO Journal, 19 4182-4190 (2000) We have examined the localization of DNA replication of the Bacillus subtilis phage f29 by immunofluorescence. To determine where phage replication was localized within infected c... [more] We have examined the localization of DNA replication of the Bacillus subtilis phage f29 by immunofluorescence. To determine where phage replication was localized within infected cells, we examined the distribution of phage replication proteins and the sites of incorporation of nucleotide analogues into phage DNA. On initiation of replication, the phage DNA localized to a single focus within the cell, nearly always towards one end of the host cell nucleoid. At later stages of the infection cycle, phage replication was found to have redistributed to multiple sites around the periphery of the nucleoid, just under the cell membrane. Towards the end of the cycle, phage DNA was once again redistributed to become located within the bulk of the nucleoid. Efficient redistribution of replicating phage DNA from the initial replication site to various sites surrounding the nucleoid was found to be dependent on the phage protein p16.7.
|
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2000 |
Lewis PJ, Thaker SD, Errington J, 'Compartmentalization of transcription and translation in Bacillus subtilis', EMBO Journal, 19 710-718 (2000) Using fusions of green fluorescent protein to subunits of RNA polymerase (RNAP) and ribosomes, we have investigated the subcellular localization of the transcriptional and transla... [more] Using fusions of green fluorescent protein to subunits of RNA polymerase (RNAP) and ribosomes, we have investigated the subcellular localization of the transcriptional and translational machinery in the bacterium Bacillus subtilis. Unexpectedly, we found that RNAP resides principally within the nucleoid. Conversely, ribosomes localized almost exclusively outside the nucleoid, concentrating particularly towards sites of cell division. This zonal localization was not dependent on cell division and is probably due, at least in part, to exclusion from the nucleoid. Dual labelling of RNAP and ribosomes was used to confirm the spatial separation of the two processes. We conclude that, even in the absence of a nuclear membrane, transcription and translation occur predominantly in separate functional domains. At higher growth rates, concentrations of RNAP developed, probably representing the sites of rRNA synthesis. These may represent a further spatial specialization, possibly equivalent to the eukaryotic nucleolus.
|
||||||||||
1999 |
Lewis PJ, Marston AL, 'GFP vectors for controlled expression and dual labelling of protein fusions in Bacillus subtilis', Gene, 227 101-109 (1999) We report the development of a series of plasmid vectors for the construction of fusions to mutants of the intrinsically fluorescent green fluorescent protein, GFPmut1 (Cormack et... [more] We report the development of a series of plasmid vectors for the construction of fusions to mutants of the intrinsically fluorescent green fluorescent protein, GFPmut1 (Cormack et al., 1996. Gene 173, 33-38) and GFPuv (Crameri et al., 1996. Nature Biotechnology 14, 315-319). Both N- and C-terminal fusions can be produced, and their expression can be finely controlled from the inducible Pxyl promoter following double crossover integration into the amyE locus of the Bacillus subtilis chromosome. Other vectors designed for single crossover insertion into the chromosome allow downstream genes to be placed under inducible control. We also show that fusions to GFPmut1 and GFPuv can be co-localized within the cell by virtue of their different excitation spectra.
|
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1998 |
Lewis PJ, Wu LJ, Errington J, 'Establishment of prespore-specific gene expression in Bacillus subtilis: Localization of SpoIIE phosphatase and initiation of compartment-specific proteolysis', Journal of Bacteriology, 180 3276-3284 (1998) Immunofluorescence microscopy was used to study the establishment of compartment-specific transcription during sporulation in Bacillus subtilis. Analysis of the distribution of th... [more] Immunofluorescence microscopy was used to study the establishment of compartment-specific transcription during sporulation in Bacillus subtilis. Analysis of the distribution of the anti-anti-sigma factor, SpoIIAA, in a variety of mutant backgrounds supports a model in which the SpoIIE phosphatase, which activates SpoIIAA by dephosphorylation, is sequestered onto the prespore face of the asymmetric septum. Thus, prespore-specific gene expression apparently arises as a result of the compartmentalization of SpoIIE protein. The results also suggest the existence of at least two compartment-specific programs of proteolysis, one dependent on the mother cell-specific sigma factor s(E) and the other dependent on the prespore- specific sigma factor s(F).
|
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Show 76 more journal articles |
Conference (21 outputs)
Year | Citation | Altmetrics | Link |
---|---|---|---|
2008 | Brzoska AJ, Turner K, Robinson A, Dixon N, Harry L, Lewis PJ, 'Identification of novel protein-protein interactions in Acinetobacter spp', ASM 2008 Melbourne: Program and Abstracts Book, Melbourne, VIC (2008) [E3] | ||
2008 | Robinson A, Brzoska AJ, Turner K, Harry L, Lewis PJ, Dixon N, 'DNA replication in pathogenic bacteria: Understanding and disrupting protein-protein interactions', ASM 2008 Melbourne: Program and Abstracts Book, Melbourne, VIC (2008) [E3] | ||
2008 | Turner K, Brzoska AJ, Robinson A, Firth N, Lewis PJ, Dixon N, Harry L, 'Isolation of novel cell division complexes using tandem affinity purification', ASM 2008 Melbourne: Program and Abstracts Book, Melbourne, VIC (2008) [E3] | ||
2008 | Withers RW, Brzoska AJ, Lewis PJ, 'Establishment of an in vitro transcription assay for Acinetobacter spp', ASM 2008 Melbourne: Program and Abstracts Book, Melbourne, VIC (2008) [E3] | ||
2008 | Brzoska AJ, Lewis PJ, 'Identification of novel protein-protein interactions in S. aureus', International Symposium on Staphylococci and Staphylococcal Infections: Abstracts, Cairns, QLD (2008) [E3] | ||
2008 | Lewis PJ, 'Subcellular localization of transcription factors', Society for General Microbiology Spring Meeting Abstracts, Edinburgh, Scotland (2008) [E3] | ||
2007 | Johnston EB, Griffith R, Lewis PJ, 'The design and testing of inhibitors of interactions between essential transcription factors and RNA polymerase', 32nd Lorne Conference on Protein Structure and Function. Abstracts, Lorne, Vic (2007) [E3] | ||
2007 | Lewis PJ, Meredith DH, Plank MW, 'Selective removal of integral membrane proteins from the mid-cell site at the onset of cell division', 4th Conference on Functional Genomics of Gram-Positive Microorganisms. Abstracts, Tirrenia, Pisa, Italy (2007) [E3] | ||
2007 | Yang X, Lewis PJ, 'Structure of transcription factor NUSA-RNA polymerase complex', ASM 2007 Adelaide Program and Abstracts Book, Adelaide, SA (2007) [E3] | ||
2007 | Lewis PJ, Doherty GP, Meredith DH, 'Transcription factor dynamics', ASM 2007 Adelaide Program and Abstracts Book, Adelaide, SA (2007) [E3] | ||
2007 | Harriott K, Lewis PJ, 'Characterisation of the interaction between PcrA and the b sub-unit of RNA polymerase', BacPath 9 : The Molecular Biology of Bacterial Pathogens. Abstracts, Lorne, VIC (2007) [E3] | ||
2007 | Johnston EB, Griffith R, Lewis PJ, 'The design and testing of inhibitors of interactions between essential transcription factors and RNA polymerase', BacPath 9 : The Molecular Biology of Bacterial Pathogens. Abstracts, Lorne, VIC (2007) [E3] | ||
2004 | MacDougall IJA, Lewis PJ, Griffith R, 'Molecular modelling of the RNA polymerase complex in Bacillus subtilis', MM2004: 9th Molecular Modelling Workshop, Sydney, Australia (2004) [E3] | ||
2004 | Lewis PJ, 'Subcellular organization of transcription in bacteria', ASM 2004 Sydney National Conference, Sydney, Australia (2004) [E3] | ||
2004 | Doherty GP, Meredith DH, Lewis PJ, 'Composition of Transcription complexes in Bacillus subtilis', ASM 2004 Sydney National Conference, Sydney, Australia (2004) [E3] | ||
2004 | Martin S, MacDougall IJA, Griffith R, Lewis PJ, 'Investigation of the interactions between transcription factor NusA with RNA polymerase in Bacillus subtilis', ASM 2004 Sydney National Conference, Sydney, Australia (2004) [E3] | ||
2004 | Migocki MD, Lewis PJ, Wake G, Harry E, 'Is chromosome replication involved in the spatial regulation of midcell division in Bacillus subtilis?', ASM 2004 Sydney National Conference, Sydney, Australia (2004) [E3] | ||
2003 | Davies KM, Dedman A, Walsh AK, Van Horck SH, Lewis PJ, 'The bacterial transcription factor NusA is preferentially recruited to sites of rRNA synthesis: Direct evidence for differential RNAP elongation complex formation in vivo', Functional Genomics of Gram-positive Microorganisms 12th International Conference on Bacilli, Baveno, Italy (2003) [E3] | ||
2002 | Lewis PJ, Davies KM, 'Analysis of transcription foci in bacillus subtilis', American Society for Microbiology - ASM Conference: Prokaryotic Development, Quebec, CANADA (2002) [E3] | ||
2002 | Lewis PJ, Harry E, 'Cellular targeting of proteins involved in positioning the division site in bacteria', Proceedings of the Australian Society for Biochemistry and Molecular Biology, Sydney, NSW (2002) [E3] | ||
2001 | Lewis PJ, 'Compartmentalisation of transcription and translation in bacteria', Australian Society for Biochemistry & Molecular Biology Incorp - Combio 2001, Canberra ACT (2001) [E3] | ||
Show 18 more conferences |
Grants and Funding
Summary
Number of grants | 50 |
---|---|
Total funding | $26,515,806 |
Click on a grant title below to expand the full details for that specific grant.
20191 grants / $24,431
Establishment of an Antimicrobial Research Taskforce in NSW: NUW ART$24,431
Funding body: NUW Alliance
Funding body | NUW Alliance |
---|---|
Project Team | Professor Peter Lewis, Associate Professor Ian Grainge, Doctor Karl Hassan, Professor Brett Neilan, Doctor Jennette Sakoff, Associate Professor Naresh Kumar, Michael Manefield, Mark Willcox, Professor Antoine van Oijen, Professor Nicholas Dixon, Associate Professor Aaron Oakley, Dr Michael Kelso |
Scheme | 2019 Projects |
Role | Lead |
Funding Start | 2019 |
Funding Finish | 2019 |
GNo | G1801287 |
Type Of Funding | C3111 - Aust For profit |
Category | 3111 |
UON | Y |
20162 grants / $41,980
Distributed facility for fragment based drug discovery$25,980
Funding body: University of Newcastle
Funding body | University of Newcastle |
---|---|
Project Team | Associate Professor Martin Scanlon, Professor Joel Mackay, Associate Professor Paul Gooley, Professor Raymond Norton, Professor Nicholas Dixon, Professor Peter Lewis, Professor Peter Scammells, Professor Jacqueline Matthews, Associate Professor Aaron Oakley, Dr Anne Kwan, Associate Professor Richard Hughes, Gooley, A/Prof Paul, Hughes, A/Prof Richard, Kwan, Dr Ann, Mackay, Prof Joel, Matthews, Prof Jacqueline, Norton, Prof Raymond, Scammells, Prof Peter |
Scheme | Equipment Grant |
Role | Lead |
Funding Start | 2016 |
Funding Finish | 2016 |
GNo | G1500331 |
Type Of Funding | Internal |
Category | INTE |
UON | Y |
Engineering Candida Tropical's for Xylose Assimilation and Metabolism$16,000
Funding body: Ethanol Technologies Limited
Funding body | Ethanol Technologies Limited |
---|---|
Project Team | Professor Peter Lewis, Dr Geoff Doherty |
Scheme | Research Grant |
Role | Lead |
Funding Start | 2016 |
Funding Finish | 2016 |
GNo | G1501454 |
Type Of Funding | Grant - Aust Non Government |
Category | 3AFG |
UON | Y |
20141 grants / $20,000
Development of compounds that inhibit transcription initiation $20,000
Funding body: University of Newcastle
Funding body | University of Newcastle |
---|---|
Project Team | Professor Peter Lewis |
Scheme | Near Miss Grant |
Role | Lead |
Funding Start | 2014 |
Funding Finish | 2014 |
GNo | G1301396 |
Type Of Funding | Internal |
Category | INTE |
UON | Y |
20133 grants / $53,000
Characterisation of a macromolecular complex essential for rapid microbial cell proliferation$20,000
Funding body: University of Newcastle
Funding body | University of Newcastle |
---|---|
Project Team | Professor Peter Lewis, Doctor Xiao Yang |
Scheme | Near Miss Grant |
Role | Lead |
Funding Start | 2013 |
Funding Finish | 2013 |
GNo | G1300482 |
Type Of Funding | Internal |
Category | INTE |
UON | Y |
Characterisation of a macromolecular complex essential for rapid microbial cell proliferation$20,000
Funding body: Hunter Medical Research Institute
Funding body | Hunter Medical Research Institute |
---|---|
Project Team | Professor Peter Lewis |
Scheme | Near Miss |
Role | Lead |
Funding Start | 2013 |
Funding Finish | 2013 |
GNo | G1300698 |
Type Of Funding | C3112 - Aust Not for profit |
Category | 3112 |
UON | Y |
New Drugs to Kill Superbugs$13,000
Funding body: University of Newcastle - Faculty of Science & IT
Funding body | University of Newcastle - Faculty of Science & IT |
---|---|
Project Team | Professor Peter Lewis, Professor Adam McCluskey, Doctor Chris Gordon, Doctor Joey Ambrus |
Scheme | Strategic Small Grant |
Role | Lead |
Funding Start | 2013 |
Funding Finish | 2013 |
GNo | G1401065 |
Type Of Funding | Internal |
Category | INTE |
UON | Y |
20123 grants / $323,426
Targeting nucleic acid synthesis and cell division in gram-negative bacterial pathogens$311,439
Funding body: NHMRC (National Health & Medical Research Council)
Funding body | NHMRC (National Health & Medical Research Council) |
---|---|
Project Team | Professor Nicholas Dixon, Associate Professor Elizabeth Harry, Professor Peter Lewis, Associate Professor Aaron Oakley, Associate Professor Ian Grainge |
Scheme | Project Grant |
Role | Lead |
Funding Start | 2012 |
Funding Finish | 2014 |
GNo | G1101133 |
Type Of Funding | Aust Competitive - Commonwealth |
Category | 1CS |
UON | Y |
Ultrasonic Homogenizer System and -80 ºC Freezers for chemical and biological sample storage$10,000
Funding body: NHMRC (National Health & Medical Research Council)
Funding body | NHMRC (National Health & Medical Research Council) |
---|---|
Project Team | Professor Adam McCluskey, Professor Eileen McLaughlin, Professor Peter Lewis, Ms Belinda Nixon, Doctor Shaun Roman, Doctor Jennette Sakoff, Associate Professor Ian Grainge |
Scheme | Equipment Grant |
Role | Investigator |
Funding Start | 2012 |
Funding Finish | 2012 |
GNo | G1100986 |
Type Of Funding | Other Public Sector - Commonwealth |
Category | 2OPC |
UON | Y |
Faculty Visiting Fellowship 2012$1,987
Funding body: University of Newcastle - Faculty of Science & IT
Funding body | University of Newcastle - Faculty of Science & IT |
---|---|
Project Team | Professor Peter Lewis |
Scheme | Visiting Fellowship |
Role | Lead |
Funding Start | 2012 |
Funding Finish | 2012 |
GNo | G1401126 |
Type Of Funding | Internal |
Category | INTE |
UON | Y |
20117 grants / $2,377,498
Exploitation of bacterial transcription initiation as a target for new antimicrobials$521,706
Funding body: NHMRC (National Health & Medical Research Council)
Funding body | NHMRC (National Health & Medical Research Council) |
---|---|
Project Team | Professor Peter Lewis, Associate Professor Renate Griffith, Associate Professor Naresh Kumar |
Scheme | Project Grant |
Role | Lead |
Funding Start | 2011 |
Funding Finish | 2013 |
GNo | G1000285 |
Type Of Funding | Aust Competitive - Commonwealth |
Category | 1CS |
UON | Y |
Establishment of a comprehensive regional biophysical analysis facility $500,000
Funding body: ARC (Australian Research Council)
Funding body | ARC (Australian Research Council) |
---|---|
Project Team | Professor Peter Lewis, Professor Nicholas Dixon, Professor Eileen McLaughlin, Professor Adam McCluskey, Associate Professor Aaron Oakley, Laureate Professor Paul Foster, Laureate Professor John Aitken, Associate Professor Jennifer Beck, Dr Michael Kelso, Professor Graham Nicholson, Associate Professor Elizabeth Harry, Professor Ian Charles, Associate Professor Steven Djordjevic |
Scheme | Linkage Infrastructure Equipment & Facilities (LIEF) |
Role | Lead |
Funding Start | 2011 |
Funding Finish | 2011 |
GNo | G1000630 |
Type Of Funding | Scheme excluded from IGS |
Category | EXCL |
UON | Y |
Chemical Biology$444,961
Funding body: University of Newcastle
Funding body | University of Newcastle |
---|---|
Project Team | Professor Adam McCluskey, Doctor Warwick Belcher, Associate Professor Ian Grainge, Professor Christopher Grof, Professor Peter Lewis, Professor Eileen McLaughlin, Doctor Shaun Roman, Emeritus Professor Ray Rose, Doctor Jennette Sakoff, Associate Professor Nikki Verrills |
Scheme | Priority Research Centre |
Role | Investigator |
Funding Start | 2011 |
Funding Finish | 2013 |
GNo | G1100052 |
Type Of Funding | Internal |
Category | INTE |
UON | Y |
Structure function analysis of the NusA-RNA polymerase interaction$340,831
Funding body: ARC (Australian Research Council)
Funding body | ARC (Australian Research Council) |
---|---|
Project Team | Professor Peter Lewis, Dr Richard Lewis, Dr Jon Marles-Wright |
Scheme | Discovery Projects |
Role | Lead |
Funding Start | 2011 |
Funding Finish | 2015 |
GNo | G1000129 |
Type Of Funding | Aust Competitive - Commonwealth |
Category | 1CS |
UON | Y |
Establishment of a Biomolecular Analysis Facility$325,000
Funding body: University of Newcastle
Funding body | University of Newcastle |
---|---|
Project Team | Professor Peter Lewis, Professor Eileen McLaughlin, Professor Adam McCluskey, Laureate Professor Paul Foster, Laureate Professor John Aitken |
Scheme | Equipment Grant |
Role | Lead |
Funding Start | 2011 |
Funding Finish | 2011 |
GNo | G1000465 |
Type Of Funding | Internal |
Category | INTE |
UON | Y |
Establishment of a comprehensive regional biophysical analysis facility $230,000
Funding body: ARC (Australian Research Council)
Funding body | ARC (Australian Research Council) |
---|---|
Project Team | Professor Peter Lewis, Professor Nicholas Dixon, Professor Eileen McLaughlin, Professor Adam McCluskey, Associate Professor Aaron Oakley, Laureate Professor Paul Foster, Laureate Professor John Aitken, Associate Professor Jennifer Beck, Dr Michael Kelso, Professor Graham Nicholson, Associate Professor Elizabeth Harry, Professor Ian Charles, Associate Professor Steven Djordjevic |
Scheme | Linkage Infrastructure Equipment & Facilities (LIEF) Partner Funding |
Role | Lead |
Funding Start | 2011 |
Funding Finish | 2011 |
GNo | G1100484 |
Type Of Funding | Scheme excluded from IGS |
Category | EXCL |
UON | Y |
Eppendorf mastercycler pro with thermomixer comfort and 5430R centrifuge$15,000
Funding body: NHMRC (National Health & Medical Research Council)
Funding body | NHMRC (National Health & Medical Research Council) |
---|---|
Project Team | Professor Eileen McLaughlin, Professor Peter Lewis, Professor Adam McCluskey, Conjoint Professor Keith Jones, Professor Brett Nixon, Doctor Shaun Roman, Doctor Jennette Sakoff, Associate Professor Ian Grainge, Doctor Janet Bristow, Doctor Xiao Yang |
Scheme | Equipment Grant |
Role | Investigator |
Funding Start | 2011 |
Funding Finish | 2011 |
GNo | G1100028 |
Type Of Funding | Other Public Sector - Commonwealth |
Category | 2OPC |
UON | Y |
20093 grants / $570,000
An Advanced Mass Spectrometry Facility for Applications in Proteomics and Organic Chemistry$495,000
Funding body: ARC (Australian Research Council)
An Advanced Mass Spectrometry Facility for Applications in Proteomics and Organic Chemistry$50,000
Funding body: ARC (Australian Research Council)
LAS-4000 Chemiluminescence imaging system$25,000
Funding body: NHMRC (National Health & Medical Research Council)
Funding body | NHMRC (National Health & Medical Research Council) |
---|---|
Project Team | Professor Eileen McLaughlin, Laureate Professor John Aitken, Professor Peter Lewis, Professor Brett Nixon, Doctor Shaun Roman |
Scheme | Equipment Grant |
Role | Investigator |
Funding Start | 2009 |
Funding Finish | 2009 |
GNo | G0189852 |
Type Of Funding | Other Public Sector - Commonwealth |
Category | 2OPC |
UON | Y |
20082 grants / $107,600
X-ray crystallography resource for membrane proteins and large macromolecular complexes$93,000
Funding body: ARC (Australian Research Council)
Funding body | ARC (Australian Research Council) |
---|---|
Project Team | Professor Peter Lewis, Doctor Robyn Malby |
Scheme | Linkage Infrastructure Equipment & Facilities (LIEF) |
Role | Lead |
Funding Start | 2008 |
Funding Finish | 2008 |
GNo | G0188894 |
Type Of Funding | Scheme excluded from IGS |
Category | EXCL |
UON | Y |
Structural Analysis of Bacterial Transcription Proteins by X-ray Crystallography$14,600
Funding body: University of Newcastle
Funding body | University of Newcastle |
---|---|
Project Team | Doctor Robyn Malby, Professor Peter Lewis |
Scheme | Pilot Grant |
Role | Investigator |
Funding Start | 2008 |
Funding Finish | 2008 |
GNo | G0189075 |
Type Of Funding | Internal |
Category | INTE |
UON | Y |
20077 grants / $1,367,901
Nucleic Acid Synthesis and Cell Division in Model Pathogenic Bacteria$740,250
Funding body: NHMRC (National Health & Medical Research Council)
Funding body | NHMRC (National Health & Medical Research Council) |
---|---|
Project Team | Professor Peter Lewis, Professor Nicholas Dixon, Associate Professor Elizabeth Harry |
Scheme | Project Grant |
Role | Lead |
Funding Start | 2007 |
Funding Finish | 2009 |
GNo | G0186390 |
Type Of Funding | Aust Competitive - Commonwealth |
Category | 1CS |
UON | Y |
Towards an understanding of dynamic genetic regulation at global scale in bacteria: A systems biology approach$486,000
Funding body: NHMRC (National Health & Medical Research Council)
Funding body | NHMRC (National Health & Medical Research Council) |
---|---|
Project Team | Professor Peter Lewis |
Scheme | NHMRC - European Union Collaborative Research Grants |
Role | Lead |
Funding Start | 2007 |
Funding Finish | 2009 |
GNo | G0186507 |
Type Of Funding | Aust Competitive - Commonwealth |
Category | 1CS |
UON | Y |
Towards an understanding of dynamic transcriptional regulation at a global scale in bacteria: a system biology approach$110,250
Funding body: Department of Innovation, Industry, Science and Research
Funding body | Department of Innovation, Industry, Science and Research |
---|---|
Project Team | Professor Peter Lewis |
Scheme | International Science Linkages (ISL) Program |
Role | Lead |
Funding Start | 2007 |
Funding Finish | 2009 |
GNo | G0187015 |
Type Of Funding | Aust Competitive - Commonwealth |
Category | 1CS |
UON | Y |
RNA POLYMERASE PROTEIN INTERACTIONS AND THE REGULATION OF TRANSCRIPTION ELONGATION$16,900
Funding body: University of Newcastle
Funding body | University of Newcastle |
---|---|
Project Team | Professor Peter Lewis |
Scheme | Near Miss Grant |
Role | Lead |
Funding Start | 2007 |
Funding Finish | 2007 |
GNo | G0187186 |
Type Of Funding | Internal |
Category | INTE |
UON | Y |
Hamamatsu ORCA-AG cooled CCD camera$12,000
Funding body: NHMRC (National Health & Medical Research Council)
Funding body | NHMRC (National Health & Medical Research Council) |
---|---|
Project Team | Professor Peter Lewis |
Scheme | Equipment Grant |
Role | Lead |
Funding Start | 2007 |
Funding Finish | 2007 |
GNo | G0188190 |
Type Of Funding | Other Public Sector - Commonwealth |
Category | 2OPC |
UON | Y |
4th Conference on Functional Genomics of Gram-Positive Microorganisms, Green Park Resort, Tirrenia, Italy, 24/6/2007 - 28/6/2007$2,500
Funding body: University of Newcastle
Funding body | University of Newcastle |
---|---|
Project Team | Professor Peter Lewis |
Scheme | Travel Grant |
Role | Lead |
Funding Start | 2007 |
Funding Finish | 2007 |
GNo | G0187783 |
Type Of Funding | Internal |
Category | INTE |
UON | Y |
Towards an understanding of dynamic transcriptional regulation at global scale in bacteria: a systems biology approach. BaSysBio$1
Funding body: European Commission, European Union
Funding body | European Commission, European Union |
---|---|
Project Team | Professor Peter Lewis |
Scheme | Sixth Framework Programme |
Role | Lead |
Funding Start | 2007 |
Funding Finish | 2007 |
GNo | G0186876 |
Type Of Funding | Not Known |
Category | UNKN |
UON | Y |
20062 grants / $20,273,000
Towards and understanding of dynamic genetic regulation at a global scale in bacteria: a Systems biology approach$20,000,000
Funding body: European Commission, European Union
Funding body | European Commission, European Union |
---|---|
Project Team | Philippe Noirot |
Scheme | 6th Framework |
Role | Investigator |
Funding Start | 2006 |
Funding Finish | 2007 |
GNo | |
Type Of Funding | International - Non Competitive |
Category | 3IFB |
UON | N |
Structural analysis and functional inactivation of bacterial transcription complexes$273,000
Funding body: ARC (Australian Research Council)
Funding body | ARC (Australian Research Council) |
---|---|
Project Team | Professor Peter Lewis, Conjoint Associate Professor Renate Griffith, Dr Richard Lewis |
Scheme | Discovery Projects |
Role | Lead |
Funding Start | 2006 |
Funding Finish | 2008 |
GNo | G0185244 |
Type Of Funding | Aust Competitive - Commonwealth |
Category | 1CS |
UON | Y |
20055 grants / $274,064
High throughput proteomics - Thermo Finnigan ProteomeX LCQ Integrated Proteomicis Workstation$207,189
Funding body: ARC (Australian Research Council)
Funding body | ARC (Australian Research Council) |
---|---|
Project Team | Laureate Professor John Aitken, Professor Eileen McLaughlin, Professor Peter Lewis, Conjoint Associate Professor Renate Griffith, Emeritus Professor Ray Rose, Emeritus Professor John Patrick, Associate Professor David McCurdy, Professor Adam McCluskey, Emeritus Professor Ellak Von Nagy-Felsobuki, Emeritus Professor Peter Dunkley, Associate Professor Phillip Dickson, Emeritus Professor John Rostas, Emeritus Professor Leonie Ashman, Professor Gordon Burns, Laureate Professor Paul Foster, Conjoint Professor Peter Gibson, Laureate Professor Roger Smith, Professor Alistair Sim, Associate Professor Paul Tooney, Dr Fraser Ross |
Scheme | Linkage Infrastructure Equipment & Facilities (LIEF) |
Role | Investigator |
Funding Start | 2005 |
Funding Finish | 2005 |
GNo | G0184185 |
Type Of Funding | Scheme excluded from IGS |
Category | EXCL |
UON | Y |
Structural analysis of bacillus subtilis RNA polymerase elongation complexes$25,064
Funding body: ARC (Australian Research Council)
Funding body | ARC (Australian Research Council) |
---|---|
Project Team | Professor Peter Lewis, Dr Richard Lewis, Assoc. Prof Alasdair McDowall |
Scheme | Linkage International Award |
Role | Lead |
Funding Start | 2005 |
Funding Finish | 2007 |
GNo | G0184314 |
Type Of Funding | Aust Competitive - Commonwealth |
Category | 1CS |
UON | Y |
2005 RIBG allocation$20,811
Funding body: University of Newcastle
Funding body | University of Newcastle |
---|---|
Project Team | Professor Peter Lewis |
Scheme | Research Infrastructure Block Grant (RIBG) |
Role | Lead |
Funding Start | 2005 |
Funding Finish | 2005 |
GNo | G0185832 |
Type Of Funding | Internal |
Category | INTE |
UON | Y |
Structural Analysis and Functional Inactivation of Bacillus Subtilis RNA Polymerase$13,000
Funding body: University of Newcastle
Funding body | University of Newcastle |
---|---|
Project Team | Professor Peter Lewis, Conjoint Associate Professor Renate Griffith |
Scheme | Project Grant |
Role | Lead |
Funding Start | 2005 |
Funding Finish | 2005 |
GNo | G0184652 |
Type Of Funding | Internal |
Category | INTE |
UON | Y |
Development of genetically engineered systems to monitor the bioavailability of pollutants in soil and water samples$8,000
Funding body: University of Newcastle
Funding body | University of Newcastle |
---|---|
Project Team | Professor Peter Lewis |
Scheme | Project Grant |
Role | Lead |
Funding Start | 2005 |
Funding Finish | 2005 |
GNo | G0184651 |
Type Of Funding | Internal |
Category | INTE |
UON | Y |
20044 grants / $795,450
Multifunctional confocal laser scanning microscope with time resolved and two photon imaging and fluorescence correlation capabilities$579,000
Funding body: Australian Research Council
Funding body | Australian Research Council |
---|---|
Project Team | E. Goldys |
Scheme | Linkage Infrastructure Equipment & Facilities (LIEF) |
Role | Investigator |
Funding Start | 2004 |
Funding Finish | 2004 |
GNo | |
Type Of Funding | Aust Competitive - Commonwealth |
Category | 1CS |
UON | N |
In vivo studies on the regulation of transcription and translation in Bacillus subtilis$210,000
Funding body: ARC (Australian Research Council)
Funding body | ARC (Australian Research Council) |
---|---|
Project Team | Professor Peter Lewis |
Scheme | Discovery Projects |
Role | Lead |
Funding Start | 2004 |
Funding Finish | 2006 |
GNo | G0182839 |
Type Of Funding | Aust Competitive - Commonwealth |
Category | 1CS |
UON | Y |
Investigation of transcript factor interaction with RNA polymerase in Bacillus subtilis$6,000
Funding body: University of Newcastle
Funding body | University of Newcastle |
---|---|
Project Team | Professor Peter Lewis, Conjoint Associate Professor Renate Griffith |
Scheme | Project Grant |
Role | Lead |
Funding Start | 2004 |
Funding Finish | 2004 |
GNo | G0183378 |
Type Of Funding | Internal |
Category | INTE |
UON | Y |
ASM 2004 Sydney, 26 Sept - 1 Oct 2004$450
Funding body: University of Newcastle
Funding body | University of Newcastle |
---|---|
Project Team | Professor Peter Lewis |
Scheme | Travel Grant |
Role | Lead |
Funding Start | 2004 |
Funding Finish | 2004 |
GNo | G0184930 |
Type Of Funding | Internal |
Category | INTE |
UON | Y |
20032 grants / $10,900
Protein-protein interactions in Bacillus subtilis.$8,500
Funding body: University of Newcastle
Funding body | University of Newcastle |
---|---|
Project Team | Professor Peter Lewis, Conjoint Associate Professor Renate Griffith |
Scheme | Project Grant |
Role | Lead |
Funding Start | 2003 |
Funding Finish | 2003 |
GNo | G0182487 |
Type Of Funding | Internal |
Category | INTE |
UON | Y |
International Conference of Functional Genomics of Gram-positive Microorganisms, Baveno, Italy 22-28 June, 2003$2,400
Funding body: University of Newcastle
Funding body | University of Newcastle |
---|---|
Project Team | Professor Peter Lewis |
Scheme | Travel Grant |
Role | Lead |
Funding Start | 2003 |
Funding Finish | 2003 |
GNo | G0183093 |
Type Of Funding | Internal |
Category | INTE |
UON | Y |
20023 grants / $17,400
Investigation of Protein-Protein Interactions in Bacillus subtillis$10,000
Funding body: University of Newcastle
Funding body | University of Newcastle |
---|---|
Project Team | Professor Peter Lewis, Conjoint Associate Professor Renate Griffith |
Scheme | Project Grant |
Role | Lead |
Funding Start | 2002 |
Funding Finish | 2002 |
GNo | G0181282 |
Type Of Funding | Internal |
Category | INTE |
UON | Y |
Subcellular localisation of translation factors in Bacillus subtillis$5,000
Funding body: University of Newcastle
Funding body | University of Newcastle |
---|---|
Project Team | Professor Peter Lewis |
Scheme | Project Grant |
Role | Lead |
Funding Start | 2002 |
Funding Finish | 2002 |
GNo | G0181368 |
Type Of Funding | Internal |
Category | INTE |
UON | Y |
Prokaryotic Development Canada, 10-14 July, 2002$2,400
Funding body: University of Newcastle
Funding body | University of Newcastle |
---|---|
Project Team | Professor Peter Lewis |
Scheme | Travel Grant |
Role | Lead |
Funding Start | 2002 |
Funding Finish | 2002 |
GNo | G0182003 |
Type Of Funding | Internal |
Category | INTE |
UON | Y |
20013 grants / $224,156
Characterisation of transcription foci, their role in chromosome segregation, and the localisation of transcription and translation factors in Bacillus subtilis$211,350
Funding body: ARC (Australian Research Council)
Funding body | ARC (Australian Research Council) |
---|---|
Project Team | Professor Peter Lewis |
Scheme | Large Grant |
Role | Lead |
Funding Start | 2001 |
Funding Finish | 2003 |
GNo | G0179272 |
Type Of Funding | Aust Competitive - Commonwealth |
Category | 1CS |
UON | Y |
Subcellular localisation of energy generating enzymes in the bacterium Bacillus subtilis.$12,000
Funding body: University of Newcastle
Funding body | University of Newcastle |
---|---|
Project Team | Professor Peter Lewis |
Scheme | Project Grant |
Role | Lead |
Funding Start | 2001 |
Funding Finish | 2001 |
GNo | G0179953 |
Type Of Funding | Internal |
Category | INTE |
UON | Y |
ComBio 2001 National Convention Centres, Canberra 1-4 October, 2001$806
Funding body: University of Newcastle
Funding body | University of Newcastle |
---|---|
Project Team | Professor Peter Lewis |
Scheme | Travel Grant |
Role | Lead |
Funding Start | 2001 |
Funding Finish | 2001 |
GNo | G0181575 |
Type Of Funding | Internal |
Category | INTE |
UON | Y |
20001 grants / $15,000
High resolution microscopic studies on the internal organisation of the bacterium Bacillus subtilis.$15,000
Funding body: University of Newcastle
Funding body | University of Newcastle |
---|---|
Project Team | Professor Peter Lewis |
Scheme | New Staff Grant |
Role | Lead |
Funding Start | 2000 |
Funding Finish | 2000 |
GNo | G0179918 |
Type Of Funding | Internal |
Category | INTE |
UON | Y |
1 grants / $20,000
Superresolution fluorescence imaging in microbiology$20,000
Funding body: University of Newcastle
Funding body | University of Newcastle |
---|---|
Project Team | Professor Antoine van Oijen, Professor Nicholas Dixon, Associate Professor Cynthia Whitchurch, Associate Professor Elizabeth Harry, Dr Lynne Turnbull, Dr Till Boecking, Associate Professor Slade Jensen, Professor Ian Paulsen, Professor Peter Lewis, Associate Professor Ian Grainge, Boecking, Dr Till, Jensen, A/Prof Slade, Turnbull, Dr Lynne |
Scheme | Equipment Grant |
Role | Lead |
Funding Start | |
Funding Finish | |
GNo | G1500398 |
Type Of Funding | Internal |
Category | INTE |
UON | Y |
Research Supervision
Number of supervisions
Current Supervision
Commenced | Level of Study | Research Title | Program | Supervisor Type |
---|---|---|---|---|
2021 | PhD | Investigating the Ferric Uptake Regulator: A Genetic Control Freak with a Taste for Iron | PhD (Biological Sciences), College of Engineering, Science and Environment, The University of Newcastle | Co-Supervisor |
2020 | PhD | Overcoming the Permeability Barrier in Gram-negative Bacterial Pathogens | PhD (Biological Sciences), College of Engineering, Science and Environment, The University of Newcastle | Principal Supervisor |
2020 | PhD | Overcoming Intrinsic Permeability and Efflux for the Development of Therapies against Gram Negative Bacteria | PhD (Biological Sciences), College of Engineering, Science and Environment, The University of Newcastle | Co-Supervisor |
2019 | PhD | Exploiting PACE Family Efflux Pumps and Their Regulatory Circuits in Synthetic Biology | PhD (Biological Sciences), College of Engineering, Science and Environment, The University of Newcastle | Co-Supervisor |
2017 | PhD | Developing a Yeast Biocatalyst for Efficient Fermentation of Lignocellulosic Sugars | PhD (Biological Sciences), College of Engineering, Science and Environment, The University of Newcastle | Principal Supervisor |
2017 | PhD | Investigating the Molecular Mechanism of the DNA Translocase FtsK and its Involvement in Site-Specific Recombination | PhD (Biological Sciences), College of Engineering, Science and Environment, The University of Newcastle | Co-Supervisor |
2016 | PhD | Characterisation and Development of RNA polymerase - s | PhD (Biological Sciences), College of Engineering, Science and Environment, The University of Newcastle | Principal Supervisor |
2006 | PhD | Structural analysis of RNA polymerase | Microbiology, University of Newcastle | Sole Supervisor |
Past Supervision
Year | Level of Study | Research Title | Program | Supervisor Type |
---|---|---|---|---|
2019 | PhD | Understanding Chromosome Dimer Resolution Systems of Pathogenic Bacteria at a Molecular Level | PhD (Biological Sciences), College of Engineering, Science and Environment, The University of Newcastle | Co-Supervisor |
2017 | PhD | Transcription Factors and Complementation Strategies in Bacteria | PhD (Biological Sciences), College of Engineering, Science and Environment, The University of Newcastle | Principal Supervisor |
2016 | PhD | Development of Novel Genetic Tools for Molecular Investigations of Pathogenic Bacteria | PhD (Biological Sciences), College of Engineering, Science and Environment, The University of Newcastle | Co-Supervisor |
2014 | PhD | Increased Understanding of the Molecular Interactions Involved in Bacterial Transcription and Recombination | PhD (Biological Sciences), College of Engineering, Science and Environment, The University of Newcastle | Principal Supervisor |
2013 | PhD | Investigation of the Transcription Complex in Acinetobacter baylyi ADP1 and the Identification of the Novel Small Acidic Transcription Factor AtfA | PhD (Biological Sciences), College of Engineering, Science and Environment, The University of Newcastle | Principal Supervisor |
2013 | PhD | Genetic Regulation of Embryo Development and Formation of Seed Storage Products in the Legume Model Medicago truncatula | PhD (Biological Sciences), College of Engineering, Science and Environment, The University of Newcastle | Co-Supervisor |
2012 | PhD | The Characterisation of the Interaction between PcrA and RNA Polymerase | PhD (Biological Sciences), College of Engineering, Science and Environment, The University of Newcastle | Sole Supervisor |
2011 | PhD | The RNA Polymerase-Q Interaction as a Target for Potential Novel Antimicrobials | PhD (Biological Sciences), College of Engineering, Science and Environment, The University of Newcastle | Principal Supervisor |
2010 | PhD | The Structure of Bacterial RNA Polymerase in Complex With the Essential Transcription Elongation Factor NusA | PhD (Biological Sciences), College of Engineering, Science and Environment, The University of Newcastle | Sole Supervisor |
2008 | PhD | The Composition of Transcription Complexes in Bacillus Subtilis | PhD (Biological Sciences), College of Engineering, Science and Environment, The University of Newcastle | Sole Supervisor |
2007 | Honours | Interaction of C-terminus of PcrA with the B subunit of RNA polymerase | Microbiology, University of Newcastle | Sole Supervisor |
2006 | Honours | Characterisation of the interaction between PcrA and the B-subunit of RNA polymerase | Microbiology, University of Newcastle | Sole Supervisor |
2005 | Honours | Development of an engineered Bacillus subtilis strain for the detection of bioavailable environmental toxicants | Microbiology, University of Newcastle | Sole Supervisor |
2005 | Honours | Characterisation of the interaction between PcrA and the beta subunit of RNA polymerase | Microbiology, University of Newcastle | Sole Supervisor |
2005 | Honours | Interaction of transcription factors NusB and NusE in Bacillus subtilis | Microbiology, University of Newcastle | Sole Supervisor |
2005 | Honours | The design and testing of inhibitors of interactions between essential transcription factors and RNA polymerase | Microbiology, University of Newcastle | Sole Supervisor |
2003 | Honours | Interactions between RNA polymerase and transcription factors: An integrated approach | Microbiology, University of Newcastle | Sole Supervisor |
2003 | Honours | Subcellular localisation of the transcription elongation factors NusB, NusG and GreA in Bacillus subtilis | Microbiology, University of Newcastle | Sole Supervisor |
2003 | Honours | Analysis of translation elongation factors EF-Tu and EF-G in Bacillus subtilis | Microbiology, University of Newcastle | Sole Supervisor |
2003 | Honours | Analysis of translation initiation factors in Bacillus subtilis | Microbiology, University of Newcastle | Sole Supervisor |
2002 | Honours | Subcellular localisation of tranlsation factors in Bacillus subtilis | Microbiology, University of Newcastle | Sole Supervisor |
2001 | Honours | Subcellular localisation of ATP synthase in Bacillus subtilis | Microbiology, University of Newcastle | Sole Supervisor |
2001 | Honours | Subcellular localisation of NusA in Bacillus subtilis | Microbiology, University of Newcastle | Sole Supervisor |
Research Projects
Transcription termination complexes 2015 - 2016
Chromosome replication, transcription and cell division in pathogenic bacteria 2007 - 2016
Inhibition of protein-protein interactions 2010 - 2016
Structure of the NusA-RNA polymerase complex 2005 - 2016
Structural analysis of transcription complexes by mass spectrometry 2009 - 2016
Transcription initiation/recycling 2007 - 2016
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Research Collaborations
The map is a representation of a researchers co-authorship with collaborators across the globe. The map displays the number of publications against a country, where there is at least one co-author based in that country. Data is sourced from the University of Newcastle research publication management system (NURO) and may not fully represent the authors complete body of work.
Country | Count of Publications | |
---|---|---|
Australia | 67 | |
United Kingdom | 20 | |
China | 6 | |
France | 5 | |
Hong Kong | 5 | |
More... |
News
Muscling RNA polymerase off the DNA
December 18, 2020
Reaping the benefits of agricultural waste
November 21, 2017
Professor Peter Lewis
Position
Professor
School of Environmental and Life Sciences
College of Engineering, Science and Environment
Focus area
Biological Sciences
Contact Details
peter.lewis@newcastle.edu.au | |
Phone | (02) 4921 5701 |
Fax | (02) 4921 5472 |
Office
Room | BG10 |
---|---|
Building | Biological Sciences. |
Location | Callaghan University Drive Callaghan, NSW 2308 Australia |