Professor Peter Lewis

Professor Peter Lewis

Professor

School of Environmental and Life Sciences (Biological Sciences)

Career Summary

Biography

My research career has focused on the gram positive bacterium Bacillus subtilis. During my PhD I purified and characterised the replication terminator protein (RTP) and its DNA binding sites (Lewis et al., J. Bacteriol 171, 3564-3567 (1989); Lewis et al., J. Mol. Biol. 214, 73-84 (1990)). These sites are regions of DNA where oppositely moving replication forks meet and newly replicated chromosomes are resolved prior to segregation. During my post-doctoral research I was responsible for the development of cell biological techniques and vectors for visualising gene expression and protein localisation in live bacterial cells (Lewis et al., PNAS 91, 3849-3853 (1994); Lewis et al., Mol Microbiol 13, 655-662 (1994); Lewis and Errington, Microbiology 142, 733-740 (1996); Lewis and Marston, Gene 227, 101-109 (1999); Feucht and Lewis, Gene 264, 289-297 (2001)).

These techniques have been widely adopted by research groups involved in microbial cell biology, and the plasmid vectors have now been distributed directly to well over 100 laboratories world-wide, although they are now also distributed by the Bacillus Genetic Stock Centre. These techniques were instrumental in determining the establishment of compartment specificity of developmentally regulated s-factors during sporulation in B. subtilis (Lewis et al., PNAS 91, 3849-3853 (1994)). I also showed that prespore specific accumulation of the transcription regulator SpoIIAA was responsible for initiation of compartment-specific gene expression during development, that this accumulation was probably due to prespore-specific activity of the phosphatase SpoIIE, and that a programme of proteolysis was initiated following this activation event (Lewis et al., Genes Cell 1, 881-894 (1996); Lewis et al., J. Bacteriol 180, 3276-3284 (1998)). I was also involved in work that showed that the highly conserved SpoIIIE (FtsK) protein is a DNA translocase that moves DNA through a division septum (Wu et al., Genes Dev 9, 1316-1326 (1995)). This was the first example of such a phenomenon, and was a very significant finding as previously it was assumed that DNA was segregated into daughter cells/different compartments prior to division septum formation. Finally, I have shown that transcription and translation are spatially separated within bacteria (Lewis et al., EMBO J 19, 710-718 (2000)). This was an unexpected result as the 2 processes were thought to be very tightly coupled in bacteria. Furthermore, transcription becomes concentrated into a sub-fraction of the bacterial nucleoid at higher growth rates. These transcription foci have been shown to be the sites of rRNA synthesis, and my laboratory is now focussing on characterising transcription complexes and their structure in detail.

Research Expertise
My research career has focused on the gram positive bacterium Bacillus subtilis. During my PhD I purified and characterised the replication terminator protein (RTP) and its DNA binding sites (Lewis et al., J. Bacteriol 171, 3564-3567 (1989); Lewis et al., J. Mol. Biol. 214, 73-84 (1990)). These sites are regions of DNA where oppositely moving replication forks meet and newly replicated chromosomes are resolved prior to segregation. During my post-doctoral research I was responsible for the development of cell biological techniques and vectors for visualising gene expression and protein localisation in live bacterial cells (Lewis et al., PNAS 91, 3849-3853 (1994); Lewis et al., Mol Microbiol 13, 655-662 (1994); Lewis and Errington, Microbiology 142, 733-740 (1996); Lewis and Marston, Gene 227, 101-109 (1999); Feucht and Lewis, Gene 264, 289-297 (2001)). These techniques have been widely adopted by research groups involved in microbial cell biology, and the plasmid vectors have now been distributed directly to well over 100 laboratories world-wide, although they are now also distributed by the Bacillus Genetic Stock Centre. These techniques were instrumental in determining the establishment of compartment specificity of developmentally regulated s-factors during sporulation in B. subtilis (Lewis et al., PNAS 91, 3849-3853 (1994)). I also showed that prespore specific accumulation of the transcription regulator SpoIIAA was responsible for initiation of compartment-specific gene expression during development, that this accumulation was probably due to prespore-specific activity of the phosphatase SpoIIE, and that a programme of proteolysis was initiated following this activation event (Lewis et al., Genes Cell 1, 881-894 (1996); Lewis et al., J. Bacteriol 180, 3276-3284 (1998)). I was also involved in work that showed that the highly conserved SpoIIIE (FtsK) protein is a DNA translocase that moves DNA through a division septum (Wu et al., Genes Dev 9, 1316-1326 (1995)). This was the first example of such a phenomenon, and was a very significant finding as previously it was assumed that DNA was segregated into daughter cells/different compartments prior to division septum formation. Finally, I have shown that transcription and translation are spatially separated within bacteria (Lewis et al., EMBO J 19, 710-718 (2000)). This was an unexpected result as the 2 processes were thought to be very tightly coupled in bacteria. Furthermore, transcription becomes concentrated into a sub-fraction of the bacterial nucleoid at higher growth rates. These transcription foci have been shown to be the sites of rRNA synthesis, and my laboratory is now focussing on characterising transcription complexes and their structure in detail.

Teaching Expertise
Molecular biology, particularly bacterial. Microbiology.

Administrative Expertise
No expertise.



Qualifications

  • Doctor of Philosophy, University of Sydney
  • Bachelor of Science, University of Sheffield - UK

Keywords

  • Biochemistry
  • Microscopy
  • Molecular Biology
  • Molecular Microbiology
  • Transcription regulation

Fields of Research

Code Description Percentage
110899 Medical Microbiology not elsewhere classified 35
060199 Biochemistry and Cell Biology not elsewhere classified 50
060499 Genetics not elsewhere classified 15

Professional Experience

UON Appointment

Title Organisation / Department
Professor University of Newcastle
School of Environmental and Life Sciences
Australia

Academic appointment

Dates Title Organisation / Department
1/07/2005 -  Membership - National Convener Molecular Microbiology Special Interest Group Australian Society for Microbiology
Australia
1/07/2000 -  Senior Lecturer University of Newcastle
School of Environmental and Life Sciences
Australia

Membership

Dates Title Organisation / Department
1/01/2006 -  OzReader ARC Biological Sciences Committee
Australia
1/01/2004 -  Member of EU 6th Framework Consortium for A$20M EU 6th Framework BaSysBio Project European Union (EU) Framework Collaboration
Australia

Professional appointment

Dates Title Organisation / Department
21/07/2008 - 21/08/2012 NHMRC Grant Review Panel National Health and Medical Research Council
Australia

Awards

Research Award

Year Award
2004 International Short-Term Fellowship
Australian Academy of Sciences

Invitations

Participant

Year Title / Rationale
2007 Invited speaker
Organisation: Conference Description: Invited to speak at SGM 162nd Meeting-Edinburgh International Conference Centre
2007 Reviewer
Organisation: Various Publishers in the field of Environmental and Life Science Description: Have been invited to write reviews for top journals in field (Molecular Microbiology, Microbiology and international Review of Cytology) and 2 book chapters in the last 3 years. Work is also now appearing in Microbiology text books.

Teaching

Code Course Role Duration
BIOL3090 Molecular Biology
Faculty of Science and IT, University of Newcastle
Course coordinator/lecturer 21/02/2002 - 21/08/2016
BTEC3250 Biotech Placement
Faculty of Science and IT, University of Newcastle
Course coordinator (2012)/supervisor 21/07/2001 - 21/08/2016
BIOL3001 Advanced Lab Skills in Biology
Faculty of Science and IT, University of Newcastle
Course coordinator/Lecturer 21/02/2007 - 21/08/2016
BIOL2002 Lab Skills in Biology
Faculty of Science and Information Technology, University of Newcastle
Lecturer 21/07/2008 - 31/12/2012
BIOL3100 Microbiology
Faculty of Science and Information Technology, University of Newcastle
Course coordinator/lecturer 21/02/2016 - 21/08/2016
BIOL1003 Biology Professional Skills 1
Faculty of Science and Information Technology, University of Newcastle
Lecturer 21/07/2011 - 21/08/2015
BIOL2090 Microbial Biology
Faculty of Science and Information Technology, University of Newcastle
Lecturer/cours coordinator (up to 2010) 21/07/2001 - 21/07/2016
Edit

Publications

For publications that are currently unpublished or in-press, details are shown in italics.


Chapter (6 outputs)

Year Citation Altmetrics Link
2012 Doherty GP, Mettrick KA, Grainge IR, Lewis PJ, 'Imaging fluorescent protein fusions in live bacteria', Methods in Microbiology, Academic Press, Kidlington, Ox 107-126 (2012) [B1]
Co-authors Ian Grainge, Karla Mettrick
2012 Lewis PJ, Yang X, 'The organisation of transcription and translation', Bacillus: Cellular and Molecular Biology, Caister Academic Press, Norfolk, UK 123-150 (2012) [B1]
2008 Lewis PJ, 'Subcellular organisation in bacteria', Bacterial Physiology: A Molecular Approach, Springer, Berlin 1-42 (2008) [B1]
2007 Lewis PJ, 'The organization of transcription and translation', Bacillus: Cellular and Molecular Biology, Caister Academic Press, Norfolk, United Kingdom 135-166 (2007) [B1]
1998 Lewis PJ, Errington J, Daniel RA, Feucht A, Wu LJ, 'Regulation of prespore-specific transcription during sporulation in Bacillus subtilis', NATO ASI Series vol H 103 Molecular Microbiology, Springer-Verlag, Berlin 175-183 (1998)
1990 Wake RG, Lewis PJ, Smith MT, 'The rtp gene and termination of chromosome replication in Bacillus subtilis', Genetics and Biotechnology of Bacilli, Academic Press, Orlando, USA 99-108 (1990)
Show 3 more chapters

Journal article (74 outputs)

Year Citation Altmetrics Link
2017 de Jong L, de Koning EA, Roseboom W, Buncherd H, Wanner MJ, Dapic I, et al., 'In-Culture Cross-Linking of Bacterial Cells Reveals Large-Scale Dynamic Protein-Protein Interactions at the Peptide Level', JOURNAL OF PROTEOME RESEARCH, 16 2457-2471 (2017) [C1]
DOI 10.1021/acs.jproteome.7b00068
2017 Yang X, Luo MJ, Yeung ACM, Lewis PJ, Chan PKS, Ip M, Ma C, 'First-In-Class Inhibitor of Ribosomal RNA Synthesis with Antimicrobial Activity against Staphylococcus aureus', Biochemistry, 56 5049-5052 (2017) [C1]

© 2017 American Chemical Society. We report the discovery of the first bacterial ribosomal RNA (rRNA) synthesis inhibitor that has specific antimicrobial activity against methici... [more]

© 2017 American Chemical Society. We report the discovery of the first bacterial ribosomal RNA (rRNA) synthesis inhibitor that has specific antimicrobial activity against methicillin-resistant Staphylococcus aureus (MRSA). A pharmacophore model was constructed on the basis of the protein-protein interaction between essential bacterial rRNA transcription factors NusB and NusE and employed for an in silico screen to identify potential leads. One compound, (E)-2-{[(3-ethynylphenyl)imino]methyl}-4-nitrophenol (MC4), demonstrated antimicrobial activity against a panel of S. aureus strains, including MRSA, without significant toxicity to mammalian cells. MC4 resulted in a decrease in the rRNA level in bacteria, and the target specificity of MC4 was confirmed at the molecular level. Results obtained from this work validated the bacterial rRNA transcription machinery as a novel antimicrobial target. This approach may be extended to other factors in rRNA transcription, and MC4 could be applied as a chemical probe to dissect the relationship among MRSA infection, MRSA growth rate, and rRNA synthesis, in addition to its therapeutic potential.

DOI 10.1021/acs.biochem.7b00349
2017 Bi Y, Liu X-X, Zhang H-Y, Yang X, Liu Z-Y, Lu J, et al., 'Synthesis and Antibacterial Evaluation of Novel 3-Substituted Ocotillol-Type Derivatives as Leads', MOLECULES, 22 (2017) [C1]
DOI 10.3390/molecules22040590
Citations Scopus - 2Web of Science - 2
2017 Cossar PJ, Ma C, Gordon CP, Ambrus JI, Lewis PJ, McCluskey A, 'Identification and validation of small molecule modulators of the NusB-NusE interaction', BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 27 162-167 (2017) [C1]
DOI 10.1016/j.bmcl.2016.11.091
Citations Scopus - 1Web of Science - 1
Co-authors Adam Mccluskey
2017 Wenholz DS, Zeng M, Ma C, Mielczarek M, Yang X, Bhadbhade M, et al., 'Small molecule inhibitors of bacterial transcription complex formation', Bioorganic and Medicinal Chemistry Letters, 27 4302-4308 (2017) [C1]
DOI 10.1016/j.bmcl.2017.08.036
2017 Cossar PJ, Abdel-Hamid MK, Ma C, Sakoff JA, Trinh TN, Gordon CP, et al., 'Small-Molecule Inhibitors of the NusB-NusE Protein-Protein Interaction with Antibiotic Activity', ACS OMEGA, 2 3839-3857 (2017) [C1]
DOI 10.1021/acsomega.7b00273
Co-authors Jennette Sakoff, Adam Mccluskey
2016 Thach O, Mielczarek M, Ma C, Kutty SK, Yang X, Black DS, et al., 'From indole to pyrrole, furan, thiophene and pyridine: Search for novel small molecule inhibitors of bacterial transcription initiation complex formation', BIOORGANIC & MEDICINAL CHEMISTRY, 24 1171-1182 (2016) [C1]
DOI 10.1016/j.bmc.2016.01.040
Citations Scopus - 2Web of Science - 2
2016 Ma C, Yang X, Lewis PJ, 'Bacterial Transcription as a Target for Antibacterial Drug Development', MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS, 80 139-160 (2016) [C1]
DOI 10.1128/MMBR.00055-15
Citations Scopus - 11Web of Science - 10
2016 Keller AN, Xin Y, Boer S, Reinhardt J, Baker R, Arciszewska LK, et al., 'Activation of Xer-recombination at dif: structural basis of the FtsK gamma-XerD interaction', SCIENTIFIC REPORTS, 6 (2016) [C1]
DOI 10.1038/srep33357
Citations Scopus - 3Web of Science - 3
Co-authors Ian Grainge
2016 Ma C, Yang X, Lewis PJ, 'Bacterial Transcription Inhibitor of RNA Polymerase Holoenzyme Formation by Structure-Based Drug Design: From in Silico Screening to Validation', ACS Infectious Diseases, 2 39-46 (2016) [C1]

© 2015 American Chemical Society. Bacterial transcription is a proven target for antibacterial research. However, most of the known inhibitors targeting transcription are from na... [more]

© 2015 American Chemical Society. Bacterial transcription is a proven target for antibacterial research. However, most of the known inhibitors targeting transcription are from natural extracts or are hits from screens where the binding site remains unidentified. Using an RNA polymerase holoenzyme homology structure from the model Gram-positive organism Bacillus subtilis, we created a pharmacophore model and used it for in silico screening of a publicly available library for compounds able to inhibit holoenzyme formation. The hits demonstrated specific affinity to bacterial RNA polymerase and excellent activity using in vitro assays and showed no binding to the equivalent structure from human RNA polymerase II. The target specificity in live cells and antibacterial activity was demonstrated in microscopy and growth inhibition experiments. This is the first example of targeted inhibitor development for a bacterial RNA polymerase, outlining a complete discovery process from virtual screening to biochemical validation. This approach could serve as an appropriate platform for the future identification of inhibitors of bacterial transcription.

DOI 10.1021/acsinfecdis.5b00058
Citations Scopus - 4Web of Science - 4
2015 Mielczarek M, Thomas RV, Ma C, Kandemir H, Yang X, Bhadbhade M, et al., 'Synthesis and biological activity of novel mono-indole and mono-benzofuran inhibitors of bacterial transcription initiation complex formation', Bioorganic and Medicinal Chemistry, (2015) [C1]

Our ongoing research focused on targeting transcription initiation in bacteria has resulted in synthesis of several classes of mono-indole and mono-benzofuran inhibitors that targ... [more]

Our ongoing research focused on targeting transcription initiation in bacteria has resulted in synthesis of several classes of mono-indole and mono-benzofuran inhibitors that targeted the essential protein-protein interaction between RNA polymerase core and s 70/s A factors in bacteria. In this study, the reaction of indole-2-, indole-3-, indole-7- and benzofuran-2-glyoxyloyl chlorides with amines and hydrazines afforded a variety of glyoxyloylamides and glyoxyloylhydrazides. Similarly, condensation of 2- and 7-trichloroacetylindoles with amines and hydrazines delivered amides and hydrazides. The novel molecules were found to inhibit the RNA polymerase-s 70/s A interaction as measured by ELISA, and also inhibited the growth of both Gram-positive and Gram-negative bacteria in culture. Structure-activity relationship (SAR) studies of the mono-indole and mono-benzofuran inhibitors suggested that the hydrophilic-hydrophobic balance is an important determinant of biological activity.

DOI 10.1016/j.bmc.2015.02.037
Citations Scopus - 10Web of Science - 9
2015 Yin Z, Whittell LR, Wang Y, Jergic S, Ma C, Lewis PJ, et al., 'Bacterial Sliding Clamp Inhibitors that Mimic the Sequential Binding Mechanism of Endogenous Linear Motifs.', J Med Chem, 58 4693-4702 (2015) [C1]
DOI 10.1021/acs.jmedchem.5b00232
Citations Scopus - 7Web of Science - 6
2015 Bi Y, Yang X, Zhang T, Liu Z, Zhang X, Lu J, et al., 'Design, synthesis, nitric oxide release and antibacterial evaluation of novel nitrated ocotillol-type derivatives', European Journal of Medicinal Chemistry, 101 71-80 (2015) [C1]

© 2015 Elsevier Masson SAS. Abstract Nitric oxide (NO) and its auto-oxidation products are known to disrupt normal bacterial function and NO releasing molecules have the potentia... [more]

© 2015 Elsevier Masson SAS. Abstract Nitric oxide (NO) and its auto-oxidation products are known to disrupt normal bacterial function and NO releasing molecules have the potential to be developed as antibacterial leads in drug discovery. We have designed and synthesized a series of novel nitrated compounds by combining NO releasing groups with ocotillol-type triterpenoids, which have previously demonstrated activity only against Gram-positive bacteria. The in vitro NO release capacity and antibacterial activity were sequentially evaluated and the data showed that most of the synthesized compounds could release nitric oxide. Compound 16a, 17a and 17c, with nitrated aliphatic esters at C-3 position, displayed higher NO release than other analogues, correlating to their good antibacterial activity, in which 17c demonstrated broad-spectrum activity against both Gram positive and -negative bacteria, as well as excellent synergism at sub-minimum inhibitory concentration when using with kanamycin and chloramphenicol. Furthermore, the epifluorescent microscopic study indicated that the ocotillol-type triterpenoid core may induce NO release on the bacterial membrane. Our results demonstrate that nitrated substitutions at C-3 of ocotillol-type derivatives could provide an approach to expand their antibacterial spectrum, and that ocotillol-type triterpenoids may also be developed as appropriate carriers for NO donors in antibacterial agent discovery with low cytotoxicity.

DOI 10.1016/j.ejmech.2015.06.021
Citations Scopus - 6Web of Science - 6
2015 Ma C, Mobli M, Yang X, Keller AN, King GF, Lewis PJ, 'RNA polymerase-induced remodelling of NusA produces a pause enhancement complex.', Nucleic acids research, 43 2829-2840 (2015) [C1]
DOI 10.1093/nar/gkv108
Citations Scopus - 11Web of Science - 12
2015 Yang X, Ma C, Lewis PJ, 'Identification of inhibitors of bacterial RNA polymerase', Methods, 86 45-50 (2015) [C1]

© 2015. Very few clinically available antibiotics target bacterial RNA polymerase (RNAP) suggesting it is an underutilized target. The advent of detailed structural information o... [more]

© 2015. Very few clinically available antibiotics target bacterial RNA polymerase (RNAP) suggesting it is an underutilized target. The advent of detailed structural information of RNAP holoenzyme (HE) has allowed the design and in silico screening of novel transcription inhibitors. Here, we describe our approach for the design and testing of small molecule transcription inhibitors that work by preventing the interaction between the essential transcription initiation factor s and RNAP. With the appropriate structural information this approach can be easily modified to other essential protein-protein interactions.

DOI 10.1016/j.ymeth.2015.05.005
Citations Scopus - 5Web of Science - 5
2015 Bi Y, Ma C, Zhou Z, Zhang T, Zhang H, Zhang X, et al., 'Synthesis and antibacterial evaluation of novel hydrophilic ocotillol-type triterpenoid derivatives from 20(S)-protopanaxadiol', Records of Natural Products, 9 356-368 (2015) [C1]

© 2015 ACG Publications. All rights reserved. Triterpenoid saponins are involved in plant defense systems to inhibit bacterial invasion. A new series of hydrophilic ocotillol-typ... [more]

© 2015 ACG Publications. All rights reserved. Triterpenoid saponins are involved in plant defense systems to inhibit bacterial invasion. A new series of hydrophilic ocotillol-type triterpenoid derivatives 5-26 have been synthesized with antibacterial activity against Gram-positive bacteria, including a community associated methicillin-resistant Staphylococcus aureus (CA-MRSA; strain USA300). From this series, compounds 6 and 15 were found to be the most active, both with MIC values of 2 µg/mL against B. subtilis 168 and 8 µg/mL against S. aureus USA300, respectively. Furthermore, subsequent assays showed that compounds 6 and 15 displayed strong synergistic effects at sub-MIC levels against both S. aureus USA300 and B. subtilis 168 when combined with two commercial antibiotics, kanamycin and chloramphenicol. Preliminary structure-activity relationship studies were also performed.

Citations Scopus - 2Web of Science - 2
2014 Mielczarek M, Devakaram RV, Ma C, Yang X, Kandemir H, Purwono B, et al., 'Synthesis and biological activity of novel bis-indole inhibitors of bacterial transcription initiation complex formation', Organic and Biomolecular Chemistry, 12 2882-2894 (2014) [C1]

The increasing resistance of bacteria against clinically approved antibiotics is resulting in an alarming decrease in therapeutic options for today's clinicians. We have targ... [more]

The increasing resistance of bacteria against clinically approved antibiotics is resulting in an alarming decrease in therapeutic options for today's clinicians. We have targeted the essential interaction between bacterial RNA polymerase and s 70 /s A for the development of lead molecules exhibiting a novel mechanism of antibacterial activity. Several classes of structurally related bis-indole inhibitors of bacterial transcription initiation complex formation were synthesized and their antimicrobial activities were evaluated. Condensation of indole-7- and indole-2-carbohydrazides with 7- and 2-trichloroacetylindoles or indole-7- and indole-2-glyoxyloyl chlorides resulted in the successful synthesis of 7,7'-, 2,2'-, 2,7'- and 3,2'-linked bis-indole derivatives with -CO-NH-NH-CO- and -CO-CO-NH-NH-CO- linkers. Indole-7-glyoxyloyl chlorides were reacted with hydrazine hydrate in different ratios to afford respective -CO-CO-NH-NH-CO-CO- bis-indole or hydrazide derivatives. The resulting compounds were found to be active against the ß'-CH- s 70 /sA2.2 interaction in ELISA assays and inhibited the growth of both Gram-positive and Gram-negative bacteria. Structure-activity relationship (SAR) studies were performed in order to identify the structural features of the synthesized inhibitors required for biological activity. This journal is © the Pa rtner Organisations 2014.

DOI 10.1039/c4ob00460d
Citations Scopus - 15Web of Science - 16
2014 Keller AN, Yang X, Wiedermannová J, Delumeau O, Krásný L, Lewis PJ, 'e, a new subunit of RNA polymerase found in gram-positive bacteria.', J Bacteriol, 196 3622-3632 (2014) [C1]
DOI 10.1128/JB.02020-14
Citations Scopus - 9Web of Science - 10
2014 Withers R, Doherty GP, Jordan M, Yang X, Dixon NE, Lewis PJ, 'AtfA, a new factor in global regulation of transcription in Acinetobacter spp', Molecular Microbiology, 93 1130-1143 (2014) [C1]

© 2014 John Wiley & Sons Ltd. Acinetobacter species are widely distributed bacteria in the environment, and have recently gained notoriety as opportunistic nosocomial patho... [more]

© 2014 John Wiley & Sons Ltd. Acinetobacter species are widely distributed bacteria in the environment, and have recently gained notoriety as opportunistic nosocomial pathogens. Here we characterize a novel RNA polymerase-interacting protein named acidic transcription factor A, AtfA. It is small and highly acidic, and is widely distributed throughout the ¿ proteobacteria, including other significant pathogens in the genera Moraxella, Pseudomonas, Legionella and Vibrio. In the model species A. baylyiADP1, deletion of atfA significantly affects expression of over 500 genes, resulting in a large cell phenotype, reduced cell fitness, impaired biofilm formation and twitching motility, and increased sensitivity to antibiotics. Deletion of atfA also causes dramatically enhanced sensitivity to ethanol, which is an important growth promoter and virulence factor in Acinetobacter spp. The results suggest that auxiliary factors of RNA polymerase with important biological roles remain to be discovered.

DOI 10.1111/mmi.12723
Citations Scopus - 3Web of Science - 3
2014 Yang X, Ma C, Lewis P, 'A vector system that allows simple generation of mutant Escherichia coli RNA polymerase', PLASMID, 75 37-41 (2014) [C1]
DOI 10.1016/j.plasmid.2014.06.002
Citations Scopus - 3Web of Science - 3
2014 Kandemir H, Kutty SK, Black DS, Kumar N, Ma C, Lewis PJ, Griffith R, 'Synthesis and biological evaluation of 2,5-di(7-indolyl)-1,3,4-oxadiazoles, and 2- and 7-indolyl 2-(1,3,4-thiadiazolyl)ketones as antimicrobials', Bioorganic and Medicinal Chemistry, (2014) [C1]

A range of novel hydrazine bridged bis-indoles was prepared from readily available indole-7-glyoxyloylchlorides and 7-trichloroacetylindoles and underwent cyclodehydration to prod... [more]

A range of novel hydrazine bridged bis-indoles was prepared from readily available indole-7-glyoxyloylchlorides and 7-trichloroacetylindoles and underwent cyclodehydration to produce 2,5-di(7-indolyl)-1,3,4-oxadiazoles and a 2,2'-bi-1,3,4-oxadiazolyl with phosphoryl chloride in ethyl acetate. This efficient protocol was subsequently used for the synthesis of 2- and 7-indolyl 2-(1,3,4-thiadiazolyl)ketones from related indolyl-hydrazine carbothioamides. The synthesised bis-indoles were evaluated for their antimicrobial properties, particularly the inhibition of protein-protein complex formation between RNA polymerase and s factor and their bactericidal effect on Gram positive Bacillus subtilis and Gram negative Escherichia coli. © 2014 Elsevier Ltd. All rights reserved.

DOI 10.1016/j.bmc.2014.01.025
Citations Scopus - 8Web of Science - 7
2014 Wiedermannová J, Sudzinová P, Koval T, Rabatinová A, ¿anderova H, Ramaniuk O, et al., 'Characterization of HelD, an interacting partner of RNA polymerase from Bacillus subtilis.', Nucleic Acids Res, 42 5151-5163 (2014)
DOI 10.1093/nar/gku113
Citations Scopus - 12
2014 Bi Y, Ma C, Zhang H, Zhou Z, Yang J, Zhang Z, et al., 'Novel 3-Substituted Ocotillol-Type Triterpenoid Derivatives as Antibacterial Candidates', CHEMICAL BIOLOGY & DRUG DESIGN, 84 489-496 (2014) [C1]
DOI 10.1111/cbdd.12337
Citations Scopus - 2Web of Science - 6
2013 Zhou Z, Ma C, Zhang H, Bi Y, Chen X, Tian H, et al., 'Synthesis and biological evaluation of novel ocotillol-type triterpenoid derivatives as antibacterial agents', European Journal of Medicinal Chemistry, 68 444-453 (2013) [C1]
DOI 10.1016/j.ejmech.2013.07.041
Citations Scopus - 13Web of Science - 12
2013 Ma C, Yang X, Kandemir H, Mielczarek M, Johnston EB, Griffith R, et al., 'Inhibitors of Bacterial Transcription Initiation Complex Formation', ACS Chemical Biology, 8 1972-1980 (2013) [C1]
DOI 10.1021/cb400231p
Citations Scopus - 26Web of Science - 26
2013 Brzoska AJ, Hassan KA, de Leon EJ, Paulsen IT, Lewis PJ, 'Single-Step Selection of Drug Resistant Acinetobacter baylyi ADP1 Mutants Reveals a Functional Redundancy in the Recruitment of Multidrug Efflux Systems', PLOS ONE, 8 (2013) [C1]
DOI 10.1371/journal.pone.0056090
Citations Scopus - 11Web of Science - 10
Co-authors Karl Hassan
2012 Buescher JM, Liebermeister W, Jules M, Uhr M, Muntel J, Botella E, et al., 'Global network reorganization during dynamic adaptations of Bacillus subtilis metabolism', Science, 335 1099-1103 (2012) [C1]
DOI 10.1126/science.1206871
Citations Scopus - 142Web of Science - 130
2012 Nicolas P, Mader U, Dervyn E, Rochat T, Leduc A, Pigeonneau N, et al., 'Condition-dependent transcriptome reveals high-level regulatory architecture in Bacillus subtilis', Science, 335 1103-1106 (2012) [C1]
Citations Scopus - 333Web of Science - 329
2012 Buncherd H, Nessen MA, Nouse N, Stelder SK, Roseboom W, Dekker HL, et al., 'Selective enrichment and identification of cross-linked peptides to study 3-D structures of protein complexes by mass spectrometry', Journal of Proteomics, 75 2205-2215 (2012) [C1]
DOI 10.1016/j.jprot.2012.01.025
Citations Scopus - 10Web of Science - 11
2011 Liew ATF, Theis T, Jensen SO, Garcia-Lara J, Foster SJ, Firth N, et al., 'A simple plasmid-based system that allows rapid generation of tightly controlled gene expression in Staphylococcus aureus', Microbiology, 157 666-676 (2011) [C1]
DOI 10.1099/mic.0.045146-0
Citations Scopus - 13Web of Science - 13
2010 Yang X, Lewis PJ, 'The interaction between RNA polymerase and the elongation factor NusA', RNA Biology, 7 272-275 (2010) [C1]
Citations Scopus - 14Web of Science - 14
2010 Yang X, Lewis PJ, 'The interaction between bacterail transcription factors and RNA polymerase during the transition from initiation to elongation', Transcription, 1 1-4 (2010) [C1]
DOI 10.4161/trns.1.2.12791
Citations Scopus - 6
2010 Doherty GP, Bailey KL, Lewis PJ, 'Stage-specific fluorescence intensity of GFP and mCherry during sporulation in Bacillus Subtilis', BMC Research Notes, 3 1-8 (2010) [C1]
DOI 10.1186/1756-0500-3-303
Citations Scopus - 17
2010 Robinson A, Brzoska AJ, Turner KM, Withers RW, Harry EJ, Lewis PJ, Dixon NE, 'Essential biological processes of an emerging pathogen: DNA replication, transcription, and cell division in Acinetobacter spp', Microbiology and Molecular Biology Reviews, 74 273-297 (2010) [C1]
DOI 10.1128/MMBR.00048-09
Citations Scopus - 29Web of Science - 31
2010 Botella E, Fogg M, Jules M, Piersma S, Doherty GP, Hansen A, et al., 'pBaSysBioII: An integrative plasmid generating gfp transcriptional fusions for high-throughput analysis of gene expression in Bacillus subtilis', Microbiology, 156 1600-1608 (2010) [C1]
DOI 10.1099/mic.0.035758-0
Citations Scopus - 41Web of Science - 39
2010 Doherty GP, Fogg MJ, Wilkinson AJ, Lewis PJ, 'Small subunits of RNA polymerase: Localization, levels and implications for core enzyme composition', Microbiology, 16 3532-3543 (2010) [C1]
DOI 10.1099/mic.0.041566-0
Citations Scopus - 23Web of Science - 22
2009 Yang X, Molimau S, Doherty GP, Johnston EB, Marles-Wright J, Rothnagel R, et al., 'The structure of bacterial RNA polymerase in complex with the essential transcription elongation factor NusA', EMBO Reports, 10 997-1002 (2009) [C1]
DOI 10.1038/embor.2009.155
Citations Scopus - 39Web of Science - 37
2009 Johnston EB, Lewis PJ, Griffith R, 'The interaction of Bacillus subtilis sigma(A) with RNA polymerase', Protein Science, 18 2287-2297 (2009) [C1]
DOI 10.1002/pro.239
Citations Scopus - 20Web of Science - 21
2008 Yang X, Lewis PJ, 'Overproduction and purification of recombinant Bacillus subtilis RNA polymerase', Protein Expression and Purification, 59 86-93 (2008) [C1]
DOI 10.1016/j.pep.2008.01.006
Citations Scopus - 20Web of Science - 23
2008 Meredith DH, Plank MW, Lewis PJ, 'Different patterns of integral membrane protein localization during cell division in Bacillus subtilis', Microbiology, 154 64-71 (2008) [C1]
DOI 10.1099/mic.0.2007/013268-0
Citations Scopus - 7Web of Science - 7
2008 Lewis PJ, Doherty GP, Clarke J, 'Transcription factor dynamics', Microbiology, 154 1837-1844 (2008) [C1]
DOI 10.1099/mic.0.2008/018549-0
Citations Scopus - 7Web of Science - 10
2008 Huo N, Lewis PJ, 'Green fluorescent protein labeling of ylyA gene in Bacillus subtilis', Weishengwu Xuebao, 48 829-833 (2008) [C1]
2008 Marles-Wright J, Grant T, Delumeau O, Van Duinen G, Firbank SJ, Lewis PJ, et al., 'Molecular architecture of the 'stressosome,' a signal integration and transduction hub', Science, 322 92-96 (2008) [C1]
DOI 10.1126/science.1159572
Citations Scopus - 88Web of Science - 83
2008 Yang X, Doherty GP, Lewis PJ, 'Tandem affinity purification vectors for use in gram positive bacteria', Plasmid, 59 54-62 (2008) [C1]
DOI 10.1016/j.plasmid.2007.11.001
Citations Scopus - 3Web of Science - 3
2006 Doherty GP, Meredith DH, Lewis PJ, 'Subcellular partitioning of transcription factors in Bacillus subtilis', Journal of Bacteriology, 188 4101-4110 (2006) [C1]
DOI 10.1128/JB.01934-05
Citations Scopus - 15Web of Science - 17
2005 Davies KM, Dedman AJ, van Horck S, Lewis PJ, 'The NusA:RNA polymerase ratio is increased at sites of rRNA synthesis in Bacillus subtilis.', Mol Microbiol, 57 366-379 (2005)
DOI 10.1111/j.1365-2958.2005.04469.x
2005 MacDougall IJA, Lewis PJ, Griffith R, 'Homology modelling of RNA polymerase and associated transcription factors from Bacillus subtilis', Journal of Molecular Graphics & Modelling, 23 297-303 (2005) [C1]
DOI 10.1016/j.jmgm.2004.10.001
Citations Scopus - 7Web of Science - 8
2005 Davies KM, Dedman AJ, Van Horck SH, Lewis PJ, 'The NusA : RNA polymerase ratio is increased at sites of rRNA synthesis in Bacillus subtilis', Molecular Microbiology, 57 366-379 (2005) [C1]
DOI 10.1111/j.1365-2958.2005.04669.x
Citations Scopus - 22Web of Science - 24
2004 Migocki MD, Lewis PJ, Wake RG, Harry EJ, 'The midcell replication factory in Bacillus subtilis is highly mobile: implications for coordinating chromosome replication with other cell cycle events', Molecular Microbiology, 54 452-463 (2004) [C1]
DOI 10.1111/j.1365-2958.2004.04267.x
Citations Scopus - 41Web of Science - 48
2004 Lewis PJ, 'Bacterial subcellular architecture: recent advances and future prospects', Molecular Microbiology, 54 1135-1150 (2004) [C1]
DOI 10.1111/j.1365-2958.2004.04343.x
Citations Scopus - 32Web of Science - 33
2004 Johnson AS, Van Horck SH, Lewis PJ, 'Dynamic localization of membrance proteins in Bacillus subtilis', Microbiology, 150 2815-2824 (2004) [C1]
DOI 10.1099/mic.0.27223-0
Citations Scopus - 41Web of Science - 45
2003 Davies KM, Lewis PJ, 'Localization of rRNA Synthesis in Bacillus subtilis: Characterization of Loci Involved in Transcription Focus Formation', Journal of Bacteriology, 185 2346-2353 (2003) [C1]
DOI 10.1128/JB.185.7.2346-2353.2003
Citations Scopus - 16Web of Science - 17
2003 Harry EJ, Lewis PJ, 'Early targeting of Min proteins to the cell poles in germinated spores of Bacillus subtilis: evidence for division apparatus-independent recruitment of Min proteins to the division site', Molecular Microbiology, 47 37-48 (2003) [C1]
DOI 10.1046/j.1365-2958.2003.03253.x
Citations Scopus - 43Web of Science - 45
2001 Lewis PJ, 'Bacterial chromosome segregation', Microbiology, 147 519-526 (2001) [C1]
Citations Scopus - 20Web of Science - 21
2001 Feucht A, Lewis PJ, 'Improved plasmid vectors for the production of multiple fluorescent protein fusions in Bacillus subtilis', Gene, 264 289-297 (2001) [C1]
Citations Scopus - 55Web of Science - 54
2000 Meijer WJJ, Lewis PJ, Errington J, Salas M, 'Dynamic relocalization of phage f29 DNA during replication and the role of the viral protein p16.7', EMBO Journal, 19 4182-4190 (2000)

We have examined the localization of DNA replication of the Bacillus subtilis phage f29 by immunofluorescence. To determine where phage replication was localized within infected c... [more]

We have examined the localization of DNA replication of the Bacillus subtilis phage f29 by immunofluorescence. To determine where phage replication was localized within infected cells, we examined the distribution of phage replication proteins and the sites of incorporation of nucleotide analogues into phage DNA. On initiation of replication, the phage DNA localized to a single focus within the cell, nearly always towards one end of the host cell nucleoid. At later stages of the infection cycle, phage replication was found to have redistributed to multiple sites around the periphery of the nucleoid, just under the cell membrane. Towards the end of the cycle, phage DNA was once again redistributed to become located within the bulk of the nucleoid. Efficient redistribution of replicating phage DNA from the initial replication site to various sites surrounding the nucleoid was found to be dependent on the phage protein p16.7.

Citations Scopus - 18
2000 Lewis PJ, Thaker SD, Errington J, 'Compartmentalization of transcription and translation in Bacillus subtilis', EMBO Journal, 19 710-718 (2000)

Using fusions of green fluorescent protein to subunits of RNA polymerase (RNAP) and ribosomes, we have investigated the subcellular localization of the transcriptional and transla... [more]

Using fusions of green fluorescent protein to subunits of RNA polymerase (RNAP) and ribosomes, we have investigated the subcellular localization of the transcriptional and translational machinery in the bacterium Bacillus subtilis. Unexpectedly, we found that RNAP resides principally within the nucleoid. Conversely, ribosomes localized almost exclusively outside the nucleoid, concentrating particularly towards sites of cell division. This zonal localization was not dependent on cell division and is probably due, at least in part, to exclusion from the nucleoid. Dual labelling of RNAP and ribosomes was used to confirm the spatial separation of the two processes. We conclude that, even in the absence of a nuclear membrane, transcription and translation occur predominantly in separate functional domains. At higher growth rates, concentrations of RNAP developed, probably representing the sites of rRNA synthesis. These may represent a further spatial specialization, possibly equivalent to the eukaryotic nucleolus.

Citations Scopus - 149
2000 Lewis PJ, Thaker SD, Errington J, 'Commpartmentalization of transcription and translation in Bacillus subtilis', The EMBO Journal, 19 710-718 (2000)
1999 Lewis PJ, Marston AL, 'GFP vectors for controlled expression and dual labelling of protein fusions in Bacillus subtilis', Gene, 227 101-109 (1999)

We report the development of a series of plasmid vectors for the construction of fusions to mutants of the intrinsically fluorescent green fluorescent protein, GFPmut1 (Cormack et... [more]

We report the development of a series of plasmid vectors for the construction of fusions to mutants of the intrinsically fluorescent green fluorescent protein, GFPmut1 (Cormack et al., 1996. Gene 173, 33-38) and GFPuv (Crameri et al., 1996. Nature Biotechnology 14, 315-319). Both N- and C-terminal fusions can be produced, and their expression can be finely controlled from the inducible Pxyl promoter following double crossover integration into the amyE locus of the Bacillus subtilis chromosome. Other vectors designed for single crossover insertion into the chromosome allow downstream genes to be placed under inducible control. We also show that fusions to GFPmut1 and GFPuv can be co-localized within the cell by virtue of their different excitation spectra.

DOI 10.1016/S0378-1119(98)00580-0
Citations Scopus - 186
1998 Lewis PJ, Wu LJ, Errington J, 'Establishment of prespore-specific gene expression in Bacillus subtilis: Localization of SpoIIE phosphatase and initiation of compartment-specific proteolysis', Journal of Bacteriology, 180 3276-3284 (1998)

Immunofluorescence microscopy was used to study the establishment of compartment-specific transcription during sporulation in Bacillus subtilis. Analysis of the distribution of th... [more]

Immunofluorescence microscopy was used to study the establishment of compartment-specific transcription during sporulation in Bacillus subtilis. Analysis of the distribution of the anti-anti-sigma factor, SpoIIAA, in a variety of mutant backgrounds supports a model in which the SpoIIE phosphatase, which activates SpoIIAA by dephosphorylation, is sequestered onto the prespore face of the asymmetric septum. Thus, prespore-specific gene expression apparently arises as a result of the compartmentalization of SpoIIE protein. The results also suggest the existence of at least two compartment-specific programs of proteolysis, one dependent on the mother cell-specific sigma factor s(E) and the other dependent on the prespore- specific sigma factor s(F).

Citations Scopus - 14
1997 Lewis PJ, Errington J, 'Direct evidence for active chromosome segregation of oriC regions of the Bacillus subtilis chromosome and co-localisation with the Spo0J partitioning protein', Molecular Microbiology, 25 945-954 (1997)
Citations Scopus - 147
1996 Lewis PJ, 'Use of green fluorescent protein for detection of cell-specific gene expression and subcellular protein localisation during sporulation in Bacillu subtiis', Microbiology, 142 733-740 (1996)
Citations Scopus - 52
1996 Lewis PJ, Magnin T, Errington J, 'Compartmentalised distribution of the proteins controlling the prespoore-specific transcription factor sF of Bacillus subtilis', Genes to Cells, 1 881-894 (1996)
Citations Scopus - 38
1996 Errington J, Feucht A, Lewis PJ, Lord M, Magnin T, Najafi SMA, et al., 'Control of the cell-specificity of sF activity in Bacillus subtilis', Philosophical Transactions of the Royal Society of London: Biological Sciences, 351 537-542 (1996)
1995 Ling JW, Lewis PJ, Allmansberger R, Hauser PM, Errington J, 'A conjugation-like mechanism for prespore chromosome partitioning during sporulation in Bacillus subtilis', Genes and Development, 9 1316-1326 (1995)

Spore formation in Bacillus subtilis begins with an asymmetric cell division that superficially resembles the division of vegetative cells. Mutations in the spoIIIE gene of B. sub... [more]

Spore formation in Bacillus subtilis begins with an asymmetric cell division that superficially resembles the division of vegetative cells. Mutations in the spoIIIE gene of B. subtilis partially block partitioning of one chromosome into the smaller (prespore) compartment of the sporulating cell. Point mutations that specifically block prespore chromosome partitioning affect a carboxy-terminal domain of SpoIIIE that shows significant sequence similarity to the DNA transfer (Tra) proteins of several conjugative plasmids of Streptomyces. In wild-type sporulating cells, the prespore chromosome passes through an intermediate stage resembling the state in which spoIIIE mutant cells are blocked. The prespore chromosome is then transferred progressively through the newly formed spore septum. We propose that translocation of the prespore chromosome occurs by a mechanism that is functionally related to the conjugative transfer of plasmid DNA.

Citations Scopus - 140
1995 Wu LJ, Almansberger R, Lewis PJ, Hauser PM, Errington J, 'The B. subtilis spoIIIE protein is a DNA translocase that drives prespore chromosome segregation by a conjugation-like mechanism', Genes and Development, 9 1316-1326 (1995)
1994 Lewis PJ, Partridge SR, Errington J, 's Factors, asymmetry, and the determination of cell fate in Bacillus subtilis', Proceedings of the National Academy of Sciences of the United States of America, 91 3849-3853 (1994)

Soon after the initiation of sporulation, Bacillus subtilis divides asymmetrically to produce sister cells that have very different developmental fates. Recently, it has been prop... [more]

Soon after the initiation of sporulation, Bacillus subtilis divides asymmetrically to produce sister cells that have very different developmental fates. Recently, it has been proposed that the differential gene expression ywhich begins soon after this division is due to cell-specific activation of the transcription factors s(F) and s(E) in the prespore and the mother cell, respectively. We describe the use of a method for the localization of gene expression in individual sporulating cells that lends strong support to the cell-specific localization of s(F) and s(E) activities. The dependence of s(E) activity on integrity of the gene encoding s(F) has led to the suggestion that activation of s(F) in the prespore leads to a directional signal that triggers activation of s(E) only in the mother cell. Here we show that s(E) actually specifies the fate of the mother cell; in the absence of s(E), two prespore-like cells are made. The appearance of s(F) activity at both poles of a s(E)-deficient mutant supports the idea that s(F) normally remains latent in the mother cell and that its activation depends on some morphological or physiological feature of the prespore. We present a model for the generation of asymmetry and the establishment of cell fate in B. subtilis.

Citations Scopus - 70
1994 Lewis PJ, Partridge SR, Errington J, 'Sigma factors, asymmetry and the determination of cell fate in Bacillus subtilis', Proceedings of the National Academy of Sciences of USA, 91 3849-3853 (1994)
1994 LEWIS PJ, NWOGUH CE, BARER MR, HARWOOD CR, ERRINGTON J, 'USE OF DIGITIZED VIDEO MICROSCOPY WITH A FLUOROGENIC ENZYME-SUBSTRATE TO DEMONSTRATE CELL-SPECIFIC AND COMPARTMENT-SPECIFIC GENE-EXPRESSION IN SALMONELLA-ENTERITIDIS AND BACILLUS-SUBTILIS', MOLECULAR MICROBIOLOGY, 13 655-662 (1994)
DOI 10.1111/j.1365-2958.1994.tb00459.x
Citations Scopus - 31Web of Science - 30
1993 Langley DB, Smith MT, Lewis PJ, Wake RG, 'Protein¿nucleoside contacts in the interaction between the replication terminator protein of Bacillus subtilis and the DNA terminator', Molecular Microbiology, 10 771-779 (1993)

The interaction between the DNA replication terminator, IRI, of Bacillus subtilis and its cognate replication terminator protein (RTP) has been examined by the technique of missin... [more]

The interaction between the DNA replication terminator, IRI, of Bacillus subtilis and its cognate replication terminator protein (RTP) has been examined by the technique of missing nucleoside interference (MNI). IRI contains two adjacent binding sites (A and B) for RTP dimers. The B site is proximal to the replication fork arrest site. The present results have shown that nucleoside contacts with RTP in the two sites are very different. There are more extensive contacts of nucleosides in both strands of the B site with RTP compared with the A site. The data also strongly suggest that filling by RTP of the B site occurs first and is needed for subsequent co-operative filling of an overlapping A site. The A site alone binds RTP poorly. The findings are consistent with interaction occurring between RTP dimers bound to adjacent sites of IRI, which would explain why RTP bound to the B site alone cannot cause replication fork arrest. Copyright © 1993, Wiley Blackwell. All rights reserved

DOI 10.1111/j.1365-2958.1993.tb00947.x
Citations Scopus - 34
1991 Lewis PJ, Wake RG, 'Termination of chromosome replication in Bacillus subtilis', Research in Microbiology, 142 893-900 (1991)
Citations Scopus - 5
1990 Lewis PJ, Ralston GB, Christopherson RI, Wake RG, 'Identification of the repliction terminator protein binding sites in the terminus region of the Bacillus subtilis chromosome and stoichiometry of the binding', Journal of Molecular Biology, 214 73-84 (1990)
Citations Scopus - 42
1989 Lewis PJ, Wake RG, 'DNA and protein sequence conservation at the replication terminus in Bacillus subtilis 168 and W23', Journal of Bacteriology, 171 1402-1408 (1989)
Citations Scopus - 20
1989 Lewis PJ, Smith MT, Wake RG, 'A protein involved in termination of chromosome replication in Bacillus subtilis binds specifically to the terC site', Journal of Bacteriology, 171 3564-3567 (1989)
Citations Scopus - 26
Show 71 more journal articles

Conference (21 outputs)

Year Citation Altmetrics Link
2008 Brzoska AJ, Turner K, Robinson A, Dixon N, Harry L, Lewis PJ, 'Identification of novel protein-protein interactions in Acinetobacter spp', ASM 2008 Melbourne: Program and Abstracts Book, Melbourne, VIC (2008) [E3]
2008 Robinson A, Brzoska AJ, Turner K, Harry L, Lewis PJ, Dixon N, 'DNA replication in pathogenic bacteria: Understanding and disrupting protein-protein interactions', ASM 2008 Melbourne: Program and Abstracts Book, Melbourne, VIC (2008) [E3]
2008 Turner K, Brzoska AJ, Robinson A, Firth N, Lewis PJ, Dixon N, Harry L, 'Isolation of novel cell division complexes using tandem affinity purification', ASM 2008 Melbourne: Program and Abstracts Book, Melbourne, VIC (2008) [E3]
2008 Withers RW, Brzoska AJ, Lewis PJ, 'Establishment of an in vitro transcription assay for Acinetobacter spp', ASM 2008 Melbourne: Program and Abstracts Book, Melbourne, VIC (2008) [E3]
2008 Brzoska AJ, Lewis PJ, 'Identification of novel protein-protein interactions in S. aureus', International Symposium on Staphylococci and Staphylococcal Infections: Abstracts, Cairns, QLD (2008) [E3]
2008 Lewis PJ, 'Subcellular localization of transcription factors', Society for General Microbiology Spring Meeting Abstracts, Edinburgh, Scotland (2008) [E3]
2007 Johnston EB, Griffith R, Lewis PJ, 'The design and testing of inhibitors of interactions between essential transcription factors and RNA polymerase', 32nd Lorne Conference on Protein Structure and Function. Abstracts, Lorne, Vic (2007) [E3]
2007 Lewis PJ, Meredith DH, Plank MW, 'Selective removal of integral membrane proteins from the mid-cell site at the onset of cell division', 4th Conference on Functional Genomics of Gram-Positive Microorganisms. Abstracts, Tirrenia, Pisa, Italy (2007) [E3]
2007 Yang X, Lewis PJ, 'Structure of transcription factor NUSA-RNA polymerase complex', ASM 2007 Adelaide Program and Abstracts Book, Adelaide, SA (2007) [E3]
2007 Lewis PJ, Doherty GP, Meredith DH, 'Transcription factor dynamics', ASM 2007 Adelaide Program and Abstracts Book, Adelaide, SA (2007) [E3]
2007 Harriott K, Lewis PJ, 'Characterisation of the interaction between PcrA and the b sub-unit of RNA polymerase', BacPath 9 : The Molecular Biology of Bacterial Pathogens. Abstracts, Lorne, VIC (2007) [E3]
2007 Johnston EB, Griffith R, Lewis PJ, 'The design and testing of inhibitors of interactions between essential transcription factors and RNA polymerase', BacPath 9 : The Molecular Biology of Bacterial Pathogens. Abstracts, Lorne, VIC (2007) [E3]
2004 MacDougall IJA, Lewis PJ, Griffith R, 'Molecular modelling of the RNA polymerase complex in Bacillus subtilis', MM2004: 9th Molecular Modelling Workshop, Sydney, Australia (2004) [E3]
2004 Lewis PJ, 'Subcellular organization of transcription in bacteria', ASM 2004 Sydney National Conference, Sydney, Australia (2004) [E3]
2004 Doherty GP, Meredith DH, Lewis PJ, 'Composition of Transcription complexes in Bacillus subtilis', ASM 2004 Sydney National Conference, Sydney, Australia (2004) [E3]
2004 Martin S, MacDougall IJA, Griffith R, Lewis PJ, 'Investigation of the interactions between transcription factor NusA with RNA polymerase in Bacillus subtilis', ASM 2004 Sydney National Conference, Sydney, Australia (2004) [E3]
2004 Migocki MD, Lewis PJ, Wake G, Harry E, 'Is chromosome replication involved in the spatial regulation of midcell division in Bacillus subtilis?', ASM 2004 Sydney National Conference, Sydney, Australia (2004) [E3]
2003 Davies KM, Dedman A, Walsh AK, Van Horck SH, Lewis PJ, 'The bacterial transcription factor NusA is preferentially recruited to sites of rRNA synthesis: Direct evidence for differential RNAP elongation complex formation in vivo', Functional Genomics of Gram-positive Microorganisms 12th International Conference on Bacilli, Baveno, Italy (2003) [E3]
2002 Lewis PJ, Davies KM, 'Analysis of transcription foci in bacillus subtilis', American Society for Microbiology - ASM Conference: Prokaryotic Development, Quebec, CANADA (2002) [E3]
2002 Lewis PJ, Harry E, 'Cellular targeting of proteins involved in positioning the division site in bacteria', Proceedings of the Australian Society for Biochemistry and Molecular Biology, Sydney, NSW (2002) [E3]
2001 Lewis PJ, 'Compartmentalisation of transcription and translation in bacteria', Australian Society for Biochemistry & Molecular Biology Incorp - Combio 2001, Canberra ACT (2001) [E3]
Show 18 more conferences
Edit

Grants and Funding

Summary

Number of grants 49
Total funding $26,491,375

Click on a grant title below to expand the full details for that specific grant.


20162 grants / $45,980

Distributed facility for fragment based drug discovery$25,980

Funding body: University of Newcastle

Funding body University of Newcastle
Project Team Associate Professor Martin Scanlon, Professor Joel Mackay, Associate Professor Paul Gooley, Professor Raymond Norton, Professor Nicholas Dixon, Professor Peter Lewis, Professor Peter Scammells, Professor Jacqueline Matthews, Associate Professor Aaron Oakley, Dr Anne Kwan, Associate Professor Richard Hughes
Scheme Equipment Grant
Role Lead
Funding Start 2016
Funding Finish 2016
GNo G1500331
Type Of Funding Internal
Category INTE
UON Y

Superresolution fluorescence imaging in microbiology$20,000

Funding body: University of Newcastle

Funding body University of Newcastle
Project Team Professor Antoine van Oijen, Professor Nicholas Dixon, Associate Professor Cynthia Whitchurch, Associate Professor Elizabeth Harry, Dr Lynne Turnbull, Dr Till Boecking, Associate Professor Slade Jensen, Professor Ian Paulsen, Professor Peter Lewis, Associate Professor Ian Grainge
Scheme Equipment Grant
Role Lead
Funding Start 2016
Funding Finish 2016
GNo G1500398
Type Of Funding Internal
Category INTE
UON Y

20151 grants / $16,000

Engineering Candida Tropical's for Xylose Assimilation and Metabolism$16,000

Funding body: Ethanol Technologies Limited

Funding body Ethanol Technologies Limited
Project Team Professor Peter Lewis, Dr Geoff Doherty
Scheme Research Grant
Role Lead
Funding Start 2015
Funding Finish 2015
GNo G1501454
Type Of Funding Grant - Aust Non Government
Category 3AFG
UON Y

20141 grants / $20,000

Development of compounds that inhibit transcription initiation $20,000

Funding body: University of Newcastle

Funding body University of Newcastle
Project Team Professor Peter Lewis
Scheme Near Miss Grant
Role Lead
Funding Start 2014
Funding Finish 2014
GNo G1301396
Type Of Funding Internal
Category INTE
UON Y

20133 grants / $53,000

Characterisation of a macromolecular complex essential for rapid microbial cell proliferation$20,000

Funding body: University of Newcastle

Funding body University of Newcastle
Project Team Professor Peter Lewis, Doctor Xiao Yang
Scheme Near Miss Grant
Role Lead
Funding Start 2013
Funding Finish 2013
GNo G1300482
Type Of Funding Internal
Category INTE
UON Y

Characterisation of a macromolecular complex essential for rapid microbial cell proliferation$20,000

Funding body: Hunter Medical Research Institute

Funding body Hunter Medical Research Institute
Project Team Professor Peter Lewis
Scheme Near Miss
Role Lead
Funding Start 2013
Funding Finish 2013
GNo G1300698
Type Of Funding External
Category EXTE
UON Y

New Drugs to Kill Superbugs$13,000

Funding body: University of Newcastle - Faculty of Science & IT

Funding body University of Newcastle - Faculty of Science & IT
Project Team Professor Peter Lewis, Professor Adam McCluskey, Doctor Chris Gordon, Doctor Joey Ambrus
Scheme Strategic Small Grant
Role Lead
Funding Start 2013
Funding Finish 2013
GNo G1401065
Type Of Funding Internal
Category INTE
UON Y

20123 grants / $323,426

Targeting nucleic acid synthesis and cell division in gram-negative bacterial pathogens$311,439

Funding body: NHMRC (National Health & Medical Research Council)

Funding body NHMRC (National Health & Medical Research Council)
Project Team Professor Nicholas Dixon, Associate Professor Elizabeth Harry, Professor Peter Lewis, Associate Professor Aaron Oakley, Associate Professor Ian Grainge
Scheme Project Grant
Role Lead
Funding Start 2012
Funding Finish 2014
GNo G1101133
Type Of Funding Aust Competitive - Commonwealth
Category 1CS
UON Y

Ultrasonic Homogenizer System and -80 ºC Freezers for chemical and biological sample storage$10,000

Funding body: NHMRC (National Health & Medical Research Council)

Funding body NHMRC (National Health & Medical Research Council)
Project Team Professor Adam McCluskey, Professor Eileen McLaughlin, Professor Peter Lewis, Ms Belinda Nixon, Doctor Shaun Roman, Doctor Jennette Sakoff, Associate Professor Ian Grainge
Scheme Equipment Grant
Role Investigator
Funding Start 2012
Funding Finish 2012
GNo G1100986
Type Of Funding Other Public Sector - Commonwealth
Category 2OPC
UON Y

Faculty Visiting Fellowship 2012$1,987

Funding body: University of Newcastle - Faculty of Science & IT

Funding body University of Newcastle - Faculty of Science & IT
Project Team Professor Peter Lewis
Scheme Visiting Fellowship
Role Lead
Funding Start 2012
Funding Finish 2012
GNo G1401126
Type Of Funding Internal
Category INTE
UON Y

20117 grants / $2,377,498

Exploitation of bacterial transcription initiation as a target for new antimicrobials$521,706

Funding body: NHMRC (National Health & Medical Research Council)

Funding body NHMRC (National Health & Medical Research Council)
Project Team Professor Peter Lewis, Associate Professor Renate Griffith, Associate Professor Naresh Kumar
Scheme Project Grant
Role Lead
Funding Start 2011
Funding Finish 2013
GNo G1000285
Type Of Funding Aust Competitive - Commonwealth
Category 1CS
UON Y

Establishment of a comprehensive regional biophysical analysis facility $500,000

Funding body: ARC (Australian Research Council)

Funding body ARC (Australian Research Council)
Project Team Professor Peter Lewis, Professor Nicholas Dixon, Professor Eileen McLaughlin, Professor Adam McCluskey, Associate Professor Aaron Oakley, Laureate Professor Paul Foster, Laureate Professor John Aitken, Associate Professor Jennifer Beck, Dr Michael Kelso, Professor Graham Nicholson, Associate Professor Elizabeth Harry, Professor Ian Charles, Associate Professor Steven Djordjevic
Scheme Linkage Infrastructure Equipment & Facilities (LIEF)
Role Lead
Funding Start 2011
Funding Finish 2011
GNo G1000630
Type Of Funding Scheme excluded from IGS
Category EXCL
UON Y

Chemical Biology$444,961

Funding body: University of Newcastle

Funding body University of Newcastle
Project Team Professor Adam McCluskey, Doctor Warwick Belcher, Associate Professor Ian Grainge, Professor Christopher Grof, Professor Peter Lewis, Professor Eileen McLaughlin, Doctor Shaun Roman, Emeritus Professor Ray Rose, Doctor Jennette Sakoff, Doctor Nikki Verrills
Scheme Priority Research Centre
Role Investigator
Funding Start 2011
Funding Finish 2015
GNo G1100052
Type Of Funding Internal
Category INTE
UON Y

Structure function analysis of the NusA-RNA polymerase interaction$340,831

Funding body: ARC (Australian Research Council)

Funding body ARC (Australian Research Council)
Project Team Professor Peter Lewis, Dr Richard Lewis, Dr Jon Marles-Wright
Scheme Discovery Projects
Role Lead
Funding Start 2011
Funding Finish 2015
GNo G1000129
Type Of Funding Aust Competitive - Commonwealth
Category 1CS
UON Y

Establishment of a Biomolecular Analysis Facility$325,000

Funding body: University of Newcastle

Funding body University of Newcastle
Project Team Professor Peter Lewis, Professor Eileen McLaughlin, Professor Adam McCluskey, Laureate Professor Paul Foster, Laureate Professor John Aitken
Scheme Equipment Grant
Role Lead
Funding Start 2011
Funding Finish 2011
GNo G1000465
Type Of Funding Internal
Category INTE
UON Y

Establishment of a comprehensive regional biophysical analysis facility $230,000

Funding body: ARC (Australian Research Council)

Funding body ARC (Australian Research Council)
Project Team Professor Peter Lewis, Professor Nicholas Dixon, Professor Eileen McLaughlin, Professor Adam McCluskey, Associate Professor Aaron Oakley, Laureate Professor Paul Foster, Laureate Professor John Aitken, Associate Professor Jennifer Beck, Dr Michael Kelso, Professor Graham Nicholson, Associate Professor Elizabeth Harry, Professor Ian Charles, Associate Professor Steven Djordjevic
Scheme Linkage Infrastructure Equipment & Facilities (LIEF) Partner Funding
Role Lead
Funding Start 2011
Funding Finish 2011
GNo G1100484
Type Of Funding Scheme excluded from IGS
Category EXCL
UON Y

Eppendorf mastercycler pro with thermomixer comfort and 5430R centrifuge$15,000

Funding body: NHMRC (National Health & Medical Research Council)

Funding body NHMRC (National Health & Medical Research Council)
Project Team Professor Eileen McLaughlin, Professor Peter Lewis, Professor Adam McCluskey, Conjoint Professor Keith Jones, Professor Brett Nixon, Doctor Shaun Roman, Doctor Jennette Sakoff, Associate Professor Ian Grainge, Doctor Janet Bristow, Doctor Xiao Yang
Scheme Equipment Grant
Role Investigator
Funding Start 2011
Funding Finish 2011
GNo G1100028
Type Of Funding Other Public Sector - Commonwealth
Category 2OPC
UON Y

20093 grants / $570,000

LAS-4000 Chemiluminescence imaging system$25,000

Funding body: NHMRC (National Health & Medical Research Council)

Funding body NHMRC (National Health & Medical Research Council)
Project Team Professor Eileen McLaughlin, Laureate Professor John Aitken, Professor Peter Lewis, Professor Brett Nixon, Doctor Shaun Roman
Scheme Equipment Grant
Role Investigator
Funding Start 2009
Funding Finish 2009
GNo G0189852
Type Of Funding Other Public Sector - Commonwealth
Category 2OPC
UON Y

20082 grants / $107,600

X-ray crystallography resource for membrane proteins and large macromolecular complexes$93,000

Funding body: ARC (Australian Research Council)

Funding body ARC (Australian Research Council)
Project Team Professor Peter Lewis, Doctor Robyn Malby
Scheme Linkage Infrastructure Equipment & Facilities (LIEF)
Role Lead
Funding Start 2008
Funding Finish 2008
GNo G0188894
Type Of Funding Scheme excluded from IGS
Category EXCL
UON Y

Structural Analysis of Bacterial Transcription Proteins by X-ray Crystallography$14,600

Funding body: University of Newcastle

Funding body University of Newcastle
Project Team Doctor Robyn Malby, Professor Peter Lewis
Scheme Pilot Grant
Role Investigator
Funding Start 2008
Funding Finish 2008
GNo G0189075
Type Of Funding Internal
Category INTE
UON Y

20077 grants / $1,367,901

Nucleic Acid Synthesis and Cell Division in Model Pathogenic Bacteria$740,250

Funding body: NHMRC (National Health & Medical Research Council)

Funding body NHMRC (National Health & Medical Research Council)
Project Team Professor Peter Lewis, Professor Nicholas Dixon, Associate Professor Elizabeth Harry
Scheme Project Grant
Role Lead
Funding Start 2007
Funding Finish 2009
GNo G0186390
Type Of Funding Aust Competitive - Commonwealth
Category 1CS
UON Y

Towards an understanding of dynamic genetic regulation at global scale in bacteria: A systems biology approach$486,000

Funding body: NHMRC (National Health & Medical Research Council)

Funding body NHMRC (National Health & Medical Research Council)
Project Team Professor Peter Lewis
Scheme NHMRC - European Union Collaborative Research Grants
Role Lead
Funding Start 2007
Funding Finish 2009
GNo G0186507
Type Of Funding Aust Competitive - Commonwealth
Category 1CS
UON Y

Towards an understanding of dynamic transcriptional regulation at a global scale in bacteria: a system biology approach$110,250

Funding body: Department of Innovation, Industry, Science and Research

Funding body Department of Innovation, Industry, Science and Research
Project Team Professor Peter Lewis
Scheme International Science Linkages (ISL) Program
Role Lead
Funding Start 2007
Funding Finish 2009
GNo G0187015
Type Of Funding Aust Competitive - Commonwealth
Category 1CS
UON Y

RNA POLYMERASE PROTEIN INTERACTIONS AND THE REGULATION OF TRANSCRIPTION ELONGATION$16,900

Funding body: University of Newcastle

Funding body University of Newcastle
Project Team Professor Peter Lewis
Scheme Near Miss Grant
Role Lead
Funding Start 2007
Funding Finish 2007
GNo G0187186
Type Of Funding Internal
Category INTE
UON Y

Hamamatsu ORCA-AG cooled CCD camera$12,000

Funding body: NHMRC (National Health & Medical Research Council)

Funding body NHMRC (National Health & Medical Research Council)
Project Team Professor Peter Lewis
Scheme Equipment Grant
Role Lead
Funding Start 2007
Funding Finish 2007
GNo G0188190
Type Of Funding Other Public Sector - Commonwealth
Category 2OPC
UON Y

4th Conference on Functional Genomics of Gram-Positive Microorganisms, Green Park Resort, Tirrenia, Italy, 24/6/2007 - 28/6/2007$2,500

Funding body: University of Newcastle

Funding body University of Newcastle
Project Team Professor Peter Lewis
Scheme Travel Grant
Role Lead
Funding Start 2007
Funding Finish 2007
GNo G0187783
Type Of Funding Internal
Category INTE
UON Y

Towards an understanding of dynamic transcriptional regulation at global scale in bacteria: a systems biology approach. BaSysBio$1

Funding body: European Commission, European Union

Funding body European Commission, European Union
Project Team Professor Peter Lewis
Scheme Sixth Framework Programme
Role Lead
Funding Start 2007
Funding Finish 2007
GNo G0186876
Type Of Funding Not Known
Category UNKN
UON Y

20062 grants / $20,273,000

Towards and understanding of dynamic genetic regulation at a global scale in bacteria: a Systems biology approach$20,000,000

Funding body: European Commission, European Union

Funding body European Commission, European Union
Project Team

Philippe Noirot

Scheme 6th Framework
Role Investigator
Funding Start 2006
Funding Finish 2007
GNo
Type Of Funding International - Non Competitive
Category 3IFB
UON N

Structural analysis and functional inactivation of bacterial transcription complexes$273,000

Funding body: ARC (Australian Research Council)

Funding body ARC (Australian Research Council)
Project Team Professor Peter Lewis, Conjoint Associate Professor Renate Griffith, Dr Richard Lewis
Scheme Discovery Projects
Role Lead
Funding Start 2006
Funding Finish 2008
GNo G0185244
Type Of Funding Aust Competitive - Commonwealth
Category 1CS
UON Y

20055 grants / $274,064

Structural analysis of bacillus subtilis RNA polymerase elongation complexes$25,064

Funding body: ARC (Australian Research Council)

Funding body ARC (Australian Research Council)
Project Team Professor Peter Lewis, Dr Richard Lewis, Assoc. Prof Alasdair McDowall
Scheme Linkage International Award
Role Lead
Funding Start 2005
Funding Finish 2007
GNo G0184314
Type Of Funding Aust Competitive - Commonwealth
Category 1CS
UON Y

2005 RIBG allocation$20,811

Funding body: University of Newcastle

Funding body University of Newcastle
Project Team Professor Peter Lewis
Scheme Research Infrastructure Block Grant (RIBG)
Role Lead
Funding Start 2005
Funding Finish 2005
GNo G0185832
Type Of Funding Internal
Category INTE
UON Y

Structural Analysis and Functional Inactivation of Bacillus Subtilis RNA Polymerase$13,000

Funding body: University of Newcastle

Funding body University of Newcastle
Project Team Professor Peter Lewis, Conjoint Associate Professor Renate Griffith
Scheme Project Grant
Role Lead
Funding Start 2005
Funding Finish 2005
GNo G0184652
Type Of Funding Internal
Category INTE
UON Y

Development of genetically engineered systems to monitor the bioavailability of pollutants in soil and water samples$8,000

Funding body: University of Newcastle

Funding body University of Newcastle
Project Team Professor Peter Lewis
Scheme Project Grant
Role Lead
Funding Start 2005
Funding Finish 2005
GNo G0184651
Type Of Funding Internal
Category INTE
UON Y

20044 grants / $795,450

Multifunctional confocal laser scanning microscope with time resolved and two photon imaging and fluorescence correlation capabilities$579,000

Funding body: Australian Research Council

Funding body Australian Research Council
Project Team

E. Goldys

Scheme Linkage Infrastructure Equipment & Facilities (LIEF)
Role Investigator
Funding Start 2004
Funding Finish 2004
GNo
Type Of Funding Aust Competitive - Commonwealth
Category 1CS
UON N

In vivo studies on the regulation of transcription and translation in Bacillus subtilis$210,000

Funding body: ARC (Australian Research Council)

Funding body ARC (Australian Research Council)
Project Team Professor Peter Lewis
Scheme Discovery Projects
Role Lead
Funding Start 2004
Funding Finish 2006
GNo G0182839
Type Of Funding Aust Competitive - Commonwealth
Category 1CS
UON Y

Investigation of transcript factor interaction with RNA polymerase in Bacillus subtilis$6,000

Funding body: University of Newcastle

Funding body University of Newcastle
Project Team Professor Peter Lewis, Conjoint Associate Professor Renate Griffith
Scheme Project Grant
Role Lead
Funding Start 2004
Funding Finish 2004
GNo G0183378
Type Of Funding Internal
Category INTE
UON Y

ASM 2004 Sydney, 26 Sept - 1 Oct 2004$450

Funding body: University of Newcastle

Funding body University of Newcastle
Project Team Professor Peter Lewis
Scheme Travel Grant
Role Lead
Funding Start 2004
Funding Finish 2004
GNo G0184930
Type Of Funding Internal
Category INTE
UON Y

20032 grants / $10,900

Protein-protein interactions in Bacillus subtilis.$8,500

Funding body: University of Newcastle

Funding body University of Newcastle
Project Team Professor Peter Lewis, Conjoint Associate Professor Renate Griffith
Scheme Project Grant
Role Lead
Funding Start 2003
Funding Finish 2003
GNo G0182487
Type Of Funding Internal
Category INTE
UON Y

International Conference of Functional Genomics of Gram-positive Microorganisms, Baveno, Italy 22-28 June, 2003$2,400

Funding body: University of Newcastle

Funding body University of Newcastle
Project Team Professor Peter Lewis
Scheme Travel Grant
Role Lead
Funding Start 2003
Funding Finish 2003
GNo G0183093
Type Of Funding Internal
Category INTE
UON Y

20023 grants / $17,400

Investigation of Protein-Protein Interactions in Bacillus subtillis$10,000

Funding body: University of Newcastle

Funding body University of Newcastle
Project Team Professor Peter Lewis, Conjoint Associate Professor Renate Griffith
Scheme Project Grant
Role Lead
Funding Start 2002
Funding Finish 2002
GNo G0181282
Type Of Funding Internal
Category INTE
UON Y

Subcellular localisation of translation factors in Bacillus subtillis$5,000

Funding body: University of Newcastle

Funding body University of Newcastle
Project Team Professor Peter Lewis
Scheme Project Grant
Role Lead
Funding Start 2002
Funding Finish 2002
GNo G0181368
Type Of Funding Internal
Category INTE
UON Y

Prokaryotic Development Canada, 10-14 July, 2002$2,400

Funding body: University of Newcastle

Funding body University of Newcastle
Project Team Professor Peter Lewis
Scheme Travel Grant
Role Lead
Funding Start 2002
Funding Finish 2002
GNo G0182003
Type Of Funding Internal
Category INTE
UON Y

20013 grants / $224,156

Characterisation of transcription foci, their role in chromosome segregation, and the localisation of transcription and translation factors in Bacillus subtilis$211,350

Funding body: ARC (Australian Research Council)

Funding body ARC (Australian Research Council)
Project Team Professor Peter Lewis
Scheme Large Grant
Role Lead
Funding Start 2001
Funding Finish 2003
GNo G0179272
Type Of Funding Aust Competitive - Commonwealth
Category 1CS
UON Y

Subcellular localisation of energy generating enzymes in the bacterium Bacillus subtilis.$12,000

Funding body: University of Newcastle

Funding body University of Newcastle
Project Team Professor Peter Lewis
Scheme Project Grant
Role Lead
Funding Start 2001
Funding Finish 2001
GNo G0179953
Type Of Funding Internal
Category INTE
UON Y

ComBio 2001 National Convention Centres, Canberra 1-4 October, 2001$806

Funding body: University of Newcastle

Funding body University of Newcastle
Project Team Professor Peter Lewis
Scheme Travel Grant
Role Lead
Funding Start 2001
Funding Finish 2001
GNo G0181575
Type Of Funding Internal
Category INTE
UON Y

20001 grants / $15,000

High resolution microscopic studies on the internal organisation of the bacterium Bacillus subtilis.$15,000

Funding body: University of Newcastle

Funding body University of Newcastle
Project Team Professor Peter Lewis
Scheme New Staff Grant
Role Lead
Funding Start 2000
Funding Finish 2000
GNo G0179918
Type Of Funding Internal
Category INTE
UON Y
Edit

Research Supervision

Number of supervisions

Completed22
Current5

Total current UON EFTSL

PhD1.6

Current Supervision

Commenced Level of Study Research Title Program Supervisor Type
2017 PhD Developing a Yeast Biocatalyst for Efficient Fermentation of Lignocellulosic Sugars PhD (Biological Sciences), Faculty of Science, The University of Newcastle Principal Supervisor
2017 PhD Investigating the Molecular Mechanism of the DNA Translocase FtsK and its Involvement in Site-Specific Recombination PhD (Biological Sciences), Faculty of Science, The University of Newcastle Co-Supervisor
2016 PhD Characterisation and Development of RNA polymerase - s PhD (Biological Sciences), Faculty of Science, The University of Newcastle Principal Supervisor
2013 PhD Understanding Chromosome Dimer Resolution Systems of a Pathogenic Bacteria at a Molecular Level PhD (Biological Sciences), Faculty of Science, The University of Newcastle Co-Supervisor
2006 PhD Structural analysis of RNA polymerase Microbiology, University of Newcastle Sole Supervisor

Past Supervision

Year Level of Study Research Title Program Supervisor Type
2017 PhD Transcription Factors and Complementation Strategies in Bacteria PhD (Biological Sciences), Faculty of Science, The University of Newcastle Principal Supervisor
2016 PhD Development of Novel Genetic Tools for Molecular Investigations of Pathogenic Bacteria PhD (Biological Sciences), Faculty of Science, The University of Newcastle Co-Supervisor
2014 PhD Increased Understanding of the Molecular Interactions Involved in Bacterial Transcription and Recombination PhD (Biological Sciences), Faculty of Science, The University of Newcastle Principal Supervisor
2013 PhD Investigation of the Transcription Complex in Acinetobacter baylyi ADP1 and the Identification of the Novel Small Acidic Transcription Factor AtfA PhD (Biological Sciences), Faculty of Science, The University of Newcastle Principal Supervisor
2013 PhD Genetic Regulation of Embryo Development and Formation of Seed Storage Products in the Legume Model Medicago truncatula PhD (Biological Sciences), Faculty of Science, The University of Newcastle Co-Supervisor
2012 PhD The Characterisation of the Interaction between PcrA and RNA Polymerase PhD (Biological Sciences), Faculty of Science, The University of Newcastle Sole Supervisor
2011 PhD The RNA Polymerase-Q Interaction as a Target for Potential Novel Antimicrobials PhD (Biological Sciences), Faculty of Science, The University of Newcastle Principal Supervisor
2010 PhD The Structure of Bacterial RNA Polymerase in Complex With the Essential Transcription Elongation Factor NusA PhD (Biological Sciences), Faculty of Science, The University of Newcastle Sole Supervisor
2008 PhD The Composition of Transcription Complexes in Bacillus Subtilis PhD (Biological Sciences), Faculty of Science, The University of Newcastle Sole Supervisor
2007 Honours Interaction of C-terminus of PcrA with the B subunit of RNA polymerase Microbiology, University of Newcastle Sole Supervisor
2006 Honours Characterisation of the interaction between PcrA and the B-subunit of RNA polymerase Microbiology, University of Newcastle Sole Supervisor
2005 Honours Development of an engineered Bacillus subtilis strain for the detection of bioavailable environmental toxicants Microbiology, University of Newcastle Sole Supervisor
2005 Honours Characterisation of the interaction between PcrA and the beta subunit of RNA polymerase Microbiology, University of Newcastle Sole Supervisor
2005 Honours Interaction of transcription factors NusB and NusE in Bacillus subtilis Microbiology, University of Newcastle Sole Supervisor
2005 Honours The design and testing of inhibitors of interactions between essential transcription factors and RNA polymerase Microbiology, University of Newcastle Sole Supervisor
2003 Honours Interactions between RNA polymerase and transcription factors: An integrated approach Microbiology, University of Newcastle Sole Supervisor
2003 Honours Subcellular localisation of the transcription elongation factors NusB, NusG and GreA in Bacillus subtilis Microbiology, University of Newcastle Sole Supervisor
2003 Honours Analysis of translation elongation factors EF-Tu and EF-G in Bacillus subtilis Microbiology, University of Newcastle Sole Supervisor
2003 Honours Analysis of translation initiation factors in Bacillus subtilis Microbiology, University of Newcastle Sole Supervisor
2002 Honours Subcellular localisation of tranlsation factors in Bacillus subtilis Microbiology, University of Newcastle Sole Supervisor
2001 Honours Subcellular localisation of ATP synthase in Bacillus subtilis Microbiology, University of Newcastle Sole Supervisor
2001 Honours Subcellular localisation of NusA in Bacillus subtilis Microbiology, University of Newcastle Sole Supervisor
Edit

Research Projects

Inhibition of protein-protein interactions 2010 - 2016


Structure of the NusA-RNA polymerase complex 2005 - 2016


Structural analysis of transcription complexes by mass spectrometry 2009 - 2016


Transcription initiation/recycling 2007 - 2016


Transcription termination complexes 2015 - 2016


Chromosome replication, transcription and cell division in pathogenic bacteria 2007 - 2016


Edit

Research Collaborations

The map is a representation of a researchers co-authorship with collaborators across the globe. The map displays the number of publications against a country, where there is at least one co-author based in that country. Data is sourced from the University of Newcastle research publication management system (NURO) and may not fully represent the authors complete body of work.

Country Count of Publications
Australia 62
United Kingdom 19
China 9
Netherlands 6
France 5
More...
Edit

News

Dr Geoff Doherty

Reaping the benefits of agricultural waste

November 21, 2017

Australia’s $63 billion* agriculture sector stands to benefit from a revolutionary fuel technology that will convert multiple agricultural waste streams into valuable biofuel and green chemical products for the first time.

Professor Peter Lewis

Position

Professor
School of Environmental and Life Sciences
Faculty of Science

Focus area

Biological Sciences

Contact Details

Email peter.lewis@newcastle.edu.au
Phone (02) 4921 5701
Fax (02) 4921 5472

Office

Room BG10
Building Biological Sciences.
Location Callaghan
University Drive
Callaghan, NSW 2308
Australia
Edit