| 2021 |
Rae AE, Wei X, Flores-Rodriguez N, McCurdy DW, Collings DA, 'Super-resolution fluorescence imaging of arabidopsis thaliana transfer cell wall ingrowths using pseudo-schiff labelling adapted for the use of different dyes', Plant and Cell Physiology, 61 1775-1787 (2021) [C1]
To understand plant growth and development, it is often necessary to investigate the organization of plant cells and plant cell walls. Plant cell walls are often fluorescently lab... [more] To understand plant growth and development, it is often necessary to investigate the organization of plant cells and plant cell walls. Plant cell walls are often fluorescently labeled for confocal imaging with the dye propidium iodide using a pseudo-Schiff reaction. This reaction binds free amine groups on dye molecules to aldehyde groups on cellulose that result from oxidation with periodic acid. We tested a range of fluorescent dyes carrying free amine groups for their ability to act as pseudo-Schiff reagents. Using the low-pH solution historically used for the Schiff reaction, these alternative dyes failed to label cell walls of Arabidopsis cotyledon vascular tissue as strongly as propidium iodide but replacing the acidic solution with water greatly improved fluorescence labeling. Under these conditions, rhodamine-123 provided improved staining of plant cell walls compared to propidium iodide. We also developed protocols for pseudo-Schiff labeling with ATTO 647N-amine, a dye compatible for super-resolution Stimulated Emission Depletion (STED) imaging. ATTO 647N-amine was used for super-resolution imaging of cell wall ingrowths that occur in phloem parenchyma transfer cells of Arabidopsis, structures whose small size is only slightly larger than the resolution limit of conventional confocal microscopy. Application of surface-rendering software demonstrated the increase in plasma membrane surface area as a consequence of wall ingrowth deposition and suggests that STED-based approaches will be useful for more detailed morphological analysis of wall ingrowth formation. These improvements in pseudo-Schiff labeling for conventional confocal microscopy and STED imaging will be broadly applicable for high-resolution imaging of plant cell walls.
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| 2021 |
Idris NA, Aleamotu a M, McCurdy DW, Collings DA, 'The orchid velamen: A model system for studying patterned secondary cell wall development?', Plants, 10 (2021)
Understanding the mechanisms through which plants generate secondary cell walls is of more than academic interest: The physical properties of plant-derived materials, including ti... [more] Understanding the mechanisms through which plants generate secondary cell walls is of more than academic interest: The physical properties of plant-derived materials, including timber and textiles, all depend upon secondary wall cellulose organization. Processes controlling cellulose in the secondary cell wall and their reliance on microtubules have been documented in recent decades, but this understanding is complicated, as secondary walls normally form in the plant¿s interior where live cell imaging is more difficult. We investigated secondary wall formation in the orchid velamen, a multicellular epidermal layer found around orchid roots that consists of dead cells with lignified secondary cell walls. The patterns of cell wall ridges that form within the velamen vary between different orchid species, but immunolabelling demonstrated that wall deposition is controlled by microtubules. As these patterning events occur at the outer surface of the root, and as orchids are adaptable for tissue culture and genetic manipulation, we conclude that the orchid root velamen may indeed be a suitable model system for studying the organization of the plant cell wall. Notably, roots of the commonly grown orchid¿<i>Laelia anceps</i>¿appear ideally suited for developing this research.
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| 2020 |
Sheahan MB, Collings DA, Rose RJ, McCurdy DW, 'ACTIN7 is required for perinuclear clustering of chloroplasts during arabidopsis protoplast culture', Plants, 9 (2020) [C1]
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| 2020 |
Wei X, Nguyen STT, Collings DA, McCurdy DW, 'Sucrose regulates wall ingrowth deposition in phloem parenchyma transfer cells in Arabidopsis via affecting phloem loading activity.', Journal of Experimental Botany, 71 4690-4702 (2020) [C1]
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| 2019 |
Aleamotu a M, McCurdy DW, Collings DA, 'Phi thickenings in roots - novel secondary wall structures responsive to biotic and abiotic stresses.', Journal of Experimental Botany, 70 4631-4641 (2019) [C1]
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| 2018 |
Wu Y, Hou J, Yu F, Nguyen STT, McCurdy DW, 'Transcript Profiling Identifies NAC-Domain Genes Involved in Regulating Wall Ingrowth Deposition in Phloem Parenchyma Transfer Cells of Arabidopsis thaliana', FRONTIERS IN PLANT SCIENCE, 9 (2018) [C1]
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| 2018 |
Aleamotu'a M, Tai Y-T, McCurdy DW, Collings DA, 'Developmental Biology and Induction of Phi Thickenings by Abiotic Stress in Roots of the Brassicaceae.', Plants, 7 (2018) [C1]
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| 2017 |
Nguyen STT, McCurdy DW, 'Wall ingrowth deposition in phloem parenchyma transfer cells in Arabidopsis: Heteroblastic variations and a potential role in pathogen defence', Plant Signaling and Behavior, 12 (2017) [C1]
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| 2017 |
Nguyen STT, Greaves T, McCurdy DW, 'Heteroblastic Development of Transfer Cells Is Controlled by the microRNA miR156/SPL Module', PLANT PHYSIOLOGY, 173 1676-1691 (2017) [C1]
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| 2017 |
Rose RJ, McCurdy DW, 'New Beginnings: Mitochondrial Renewal by Massive Mitochondrial Fusion', TRENDS IN PLANT SCIENCE, 22 641-643 (2017)
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| 2016 |
Arun-Chinnappa KS, McCurdy DW, 'Identification of Candidate Transcriptional Regulators of Epidermal Transfer Cell Development in Vicia faba Cotyledons.', Front Plant Sci, 7 717 (2016) [C1]
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| 2015 |
Zhang HM, Van Helden DF, McCurdy DW, Offler CE, Patrick JW, 'Plasma Membrane Ca
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| 2015 |
Zhang HM, Imtiaz MS, Laver DR, McCurdy DW, Offler CE, Van Helden DF, Patrick JW, 'Polarized and persistent Ca
Transfer cell morphology is characterized by a polarized ingrowth wall comprising a uniform wall upon which wall ingrowth papillae develop at right angles into the cytoplasm. The ... [more] Transfer cell morphology is characterized by a polarized ingrowth wall comprising a uniform wall upon which wall ingrowth papillae develop at right angles into the cytoplasm. The hypothesis that positional information directing construction of wall ingrowth papillae is mediated by Ca2+ signals generated by spatiotemporal alterations in cytosolic Ca2+ ([Ca2+]cyt) of cells trans-differentiating to a transfer cell morphology was tested. This hypothesis was examined using Vicia faba cotyledons. On transferring cotyledons to culture, their adaxial epidermal cells synchronously trans-differentiate to epidermal transfer cells. A polarized and persistent Ca2+ signal, generated during epidermal cell trans-differentiation, was found to co-localize with the site of ingrowth wall formation. Dampening Ca2+ signal intensity, by withdrawing extracellular Ca2+ or blocking Ca2+ channel activity, inhibited formation of wall ingrowth papillae. Maintenance of Ca2+ signal polarity and persistence depended upon a rapid turnover (minutes) of cytosolic Ca2+ by co-operative functioning of plasma membrane Ca2+-permeable channels and Ca2+-ATPases. Viewed paradermally, and proximal to the cytosol-plasma membrane interface, the Ca2+ signal was organized into discrete patches that aligned spatially with clusters of Ca2+-permeable channels. Mathematical modelling demonstrated that these patches of cytosolic Ca2+ were consistent with inward-directed plumes of elevated [Ca2+]cyt. Plume formation depended upon an alternating distribution of Ca2+-permeable channels and Ca2+-ATPase clusters. On further inward diffusion, the Ca2+ plumes coalesced into a uniform Ca2+ signal. Blocking or dispersing the Ca2+ plumes inhibited deposition of wall ingrowth papillae, while uniform wall formation remained unaltered. A working model envisages that cytosolic Ca2+ plumes define the loci at which wall ingrowth papillae are deposited.
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| 2015 |
Zhang H-M, Talbot MJ, McCurdy DW, Patrick JW, Offler CE, 'Calcium-dependent depletion zones in the cortical microtubule array coincide with sites of, but do not regulate, wall ingrowth papillae deposition in epidermal transfer cells.', J Exp Bot, 66 6021-6033 (2015) [C1]
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| 2015 |
Arun-Chinnappa KS, McCurdy DW, 'De novo assembly of a genome-wide transcriptome map of Vicia faba (L) for transfer cell research', Frontiers in Plant Science, 6 (2015) [C1]
Vicia faba (L.) is an important cool-season grain legume species used widely in agriculture but also in plant physiology research, particularly as an experimental model to study t... [more] Vicia faba (L.) is an important cool-season grain legume species used widely in agriculture but also in plant physiology research, particularly as an experimental model to study transfer cell (TC) development. TCs are specialized nutrient transport cells in plants, characterized by invaginated wall ingrowths with amplified plasma membrane surface area enriched with transporter proteins that facilitate nutrient transfer. Many TCs are formed by trans-differentiation from differentiated cells at apoplasmic/symplasmic boundaries in nutrient transport. Adaxial epidermal cells of isolated cotyledons can be induced to form functional TCs, thus providing a valuable experimental system to investigate genetic regulation of TC trans-differentiation. The genome of V. faba is exceedingly large (ca. 13 Gb), however, and limited genomic information is available for this species. To provide a resource for future transcript profiling of epidermal TC differentiation, we have undertaken de novo assembly of a genome-wide transcriptome map for V faba. Illumina paired-end sequencing of total RNA pooled from different tissues and different stages, including isolated cotyledons induced to form epidermal TCs, generated 69.5 M reads, of which 65.8 M were used for assembly following trimming and quality control. Assembly using a De-Bruijn graph-based approach generated 21,297 contigs, of which 80.6% were successfully annotated against GO terms. The assembly was validated against known V faba cDNAs held in GenBank, including transcripts previously identified as being specifically expressed in epidermal cells across TC trans-differentiation. This genome-wide transcriptome map therefore provides a valuable tool for future transcript profiling of epidermal TC trans-differentiation, and also enriches the genetic resources available for this important legume crop species.
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| 2015 |
Wolfenstetter S, Chakravorty D, Kula R, Urano D, Trusov Y, Sheahan MB, et al., 'Evidence for an unusual transmembrane configuration of AGG3, a class C G subunit of Arabidopsis', Plant Journal, 81 388-398 (2015) [C1]
Heterotrimeric G proteins are crucial for the perception of external signals and subsequent signal transduction in animal and plant cells. In both model systems, the complex compr... [more] Heterotrimeric G proteins are crucial for the perception of external signals and subsequent signal transduction in animal and plant cells. In both model systems, the complex comprises one Ga, one Gß, and one G¿ subunit. However, in addition to the canonical G¿ subunits (class A), plants also possess two unusual, plant-specific classes of G¿ subunits (classes B and C) that have not yet been found in animals. These include G¿ subunits lacking the C-terminal CaaX motif (class B), which is important for membrane anchoring of the protein; the presence of such subunits gives rise to a flexible sub-population of Gß/¿ heterodimers that are not necessarily restricted to the plasma membrane. Plants also contain class C G¿ subunits, which are twice the size of canonical G¿ subunits, with a predicted transmembrane domain and a large cysteine-rich extracellular C-terminus. However, neither the presence of the transmembrane domain nor the membrane topology have been unequivocally demonstrated. Here, we provide compelling evidence that AGG3, a class C G¿ subunit of Arabidopsis, contains a functional transmembrane domain, which is sufficient but not essential for plasma membrane localization, and that the cysteine-rich C-terminus is extracellular. Significance Statement AGG3 is the prototype of an unusual G¿ subunit of the heterotrimeric G protein complex. It is a type II membrane protein having a cysteine-rich extracellular domain.
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| 2015 |
Nguyen STT, McCurdy DW, 'High-resolution confocal imaging of wall ingrowth deposition in plant transfer cells: Semi-quantitative analysis of phloem parenchyma transfer cell development in leaf minor veins of Arabidopsis', BMC Plant Biology, 15 (2015) [C1]
Background: Transfer cells (TCs) are trans-differentiated versions of existing cell types designed to facilitate enhanced membrane transport of nutrients at symplasmic/apoplasmic ... [more] Background: Transfer cells (TCs) are trans-differentiated versions of existing cell types designed to facilitate enhanced membrane transport of nutrients at symplasmic/apoplasmic interfaces. This transport capacity is conferred by intricate wall ingrowths deposited secondarily on the inner face of the primary cell wall, hence promoting the potential trans-membrane flux of solutes and consequently assigning TCs as having key roles in plant growth and productivity. However, TCs are typically positioned deep within tissues and have been studied mostly by electron microscopy. Recent advances in fluorophore labelling of plant cell walls using a modified pseudo-Schiff-propidium iodide (mPS-PI) staining procedure in combination with high-resolution confocal microscopy have allowed visualization of cellular details of individual tissue layers in whole mounts, hence enabling study of tissue and cellular architecture without the need for tissue sectioning. Here we apply a simplified version of the mPS-PI procedure for confocal imaging of cellulose-enriched wall ingrowths in vascular TCs at the whole tissue level. Results: The simplified mPS-PI staining procedure produced high-resolution three-dimensional images of individual cell types in vascular bundles and, importantly, wall ingrowths in phloem parenchyma (PP) TCs in minor veins of Arabidopsis leaves and companion cell TCs in pea. More efficient staining of tissues was obtained by replacing complex clearing procedures with a simple post-fixation bleaching step. We used this modified procedure to survey the presence of PP TCs in other tissues of Arabidopsis including cotyledons, cauline leaves and sepals. This high-resolution imaging enabled us to classify different stages of wall ingrowth development in Arabidopsis leaves, hence enabling semi-quantitative assessment of the extent of wall ingrowth deposition in PP TCs at the whole leaf level. Finally, we conducted a defoliation experiment as an example of using this approach to statistically analyze responses of PP TC development to leaf ablation. Conclusions: Use of a modified mPS-PI staining technique resulted in high-resolution confocal imaging of polarized wall ingrowth deposition in TCs. This technique can be used in place of conventional electron microscopy and opens new possibilities to study mechanisms determining polarized deposition of wall ingrowths and use reverse genetics to identify regulatory genes controlling TC trans-differentiation.
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| 2014 |
McCurdy DW, Hueros G, 'Transfer cells', Frontiers in Plant Science, 5 (2014) [C3]
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| 2013 |
Chinnappa KSA, Nguyen TTS, Hou J, Wu Y, McCurdy DW, 'Phloem parenchyma transfer cells in Arabidopsis - an experimental system to identify transcriptional regulators of wall ingrowth formation', Frontiers in Plant Science, 4 (2013) [C1]
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| 2012 |
Andriunas FA, Zhang H, Xia X, Offler CE, McCurdy DW, Patrick JW, 'Reactive oxygen species form part of a regulatory pathway initiating trans-differentiation of epidermal transfer cells in Vicia faba cotyledons', Journal of Experimental Botany, 63 3617-3630 (2012) [C1]
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| 2011 |
Chakravorty D, Trusov Y, Zhang W, Acharya BR, Sheahan MB, McCurdy DW, et al., 'An atypical heterotrimeric G-protein gamma-subunit is involved in guard cell K(+)-channel regulation and morphological development in Arabidopsis thaliana', The Plant Journal, 67 840-851 (2011) [C1]
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| 2011 |
Andriunas FA, Zhang H, Weber H, McCurdy DW, Offler CE, Patrick JW, 'Glucose and ethylene signalling pathways converge to regulate trans-differentiation of epidermal transfer cells in Vicia narbonensis cotyledons', The Plant Journal, 68 987-998 (2011) [C1]
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| 2010 |
McCurdy DW, Dibley SJ, Cahyanegara R, Martin A, Patrick JW, 'Functional characterization and RNAi-mediated suppression reveals roles for hexose transporters in sugar accumulation by tomato fruit', Molecular Plant, 3 1049-1063 (2010) [C1]
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| 2010 |
Zhou Y, Andriunas FA, Offler CE, McCurdy DW, Patrick JW, 'An epidermal-specific ethylene signal cascade regulates trans-differentiation of transfer cells in Vicia faba cotyledons', New Phytologist, 185 931-943 (2010) [C1]
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| 2010 |
Edwards J, Martin A, Andriunas FA, Offler CE, Patrick JW, McCurdy DW, 'GIGANTEA is a component of a regulatory pathway determining wall ingrowth deposition in phloem parenchyma transfer cells of Arabidopsis thaliana', The Plant Journal, 63 651-661 (2010) [C1]
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| 2009 |
Staiger CJ, Sheahan MB, Khurana P, Wang X, McCurdy DW, Blanchoin L, 'Actin filament dynamics are dominated by rapid growth and severing activity in the Arabidopsis cortical array', Journal of Cell Biology, 184 269-280 (2009) [C1]
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| 2009 |
Dibley SJ, Zhou Y, Andriunas FA, Talbot MJ, Offler CE, Patrick JW, McCurdy DW, 'Early gene expression programs accompanying trans-differentiation of epidermal cells of Vicia faba cotyledons into transfer cells', New Phytologist, 182 863-877 (2009) [C1]
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| 2008 |
McCurdy DW, Patrick JW, Offler CE, 'Wall ingrowth formation in transfer cells: Novel examples of localized wall deposition in plant cells', Current Opinion in Plant Biology, 11 653-661 (2008) [C1]
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| 2007 |
Talbot MJ, Wasteneys G, McCurdy DW, Offler CE, 'Deposition patterns of cellulose microfibrils in flange wall ingrowths of transfer cells indicate clear parallels with those of secondary wall thickenings', Functional Plant Biology, 34 307-313 (2007) [C1]
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| 2007 |
Sheahan MB, Rose RJ, McCurdy DW, 'Mechanisms of organelle inheritance in dividing plant cells', Journal of Integrative Plant Biology, 49 1208-1218 (2007) [C1]
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| 2007 |
Vaughn KC, Talbot MJ, Offler CE, McCurdy DW, 'Wall ingrowths in epidermal transfer cells of Vicia faba cotyledons are modified primary walls marked by localized accumulations of arabinogalactan proteins', Plant and Cell Physiology, 48 159-168 (2007) [C1]
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| 2007 |
Talbot MJ, Wasteneys GO, Offler CE, McCurdy DW, 'Cellulose synthesis is required for deposition of reticulate wall ingrowths in transfer cells', Plant and Cell Physiology, 48 147-158 (2007) [C1]
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| 2007 |
Sheahan MB, Rose RJ, McCurdy DW, 'Actin-filament-dependent remodeling of the vacuole in cultured mesophyll protoplasts', Protoplasma, 230 141-152 (2007) [C1]
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| 2005 |
Sheahan MB, McCurdy DW, Rose RJ, 'Mitochondria as a connected population: ensuring continuity of the mitochondrial genome during plant cell dedifferentiation through massive mitochondrial fusion', The Plant Journal, 44 744-755 (2005) [C1]
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| 2005 |
Dibley SJ, Gear ML, Yang X, Rosche E, Offler CE, McCurdy DW, Patrick JW, 'Temporal and spatial expression of hexose transporters in developing tomato (Lycopersicon esculentum) fruit', Functional Plant Biology, 32 777-785 (2005) [C1]
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| 2005 |
Baluska F, Liners F, Hlavacka A, Schlicht M, Van Cutsem P, McCurdy DW, Menzel D, 'Cell wall pectins and xyloglucans are internalized into dividing root cells and accumulate within cell plates during cytokinesis', Protoplasma, 225 141-155 (2005) [C1]
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| 2004 |
Sheahan MB, Staiger C, Rose RJ, McCurdy DW, 'A Green Fluorescent Protein Fusion to Actin-Binding Domain 2 of Arabidopsis Fimbrin Highlights New Features of a Dynamic Actin Cytoskeleton in Live Plant Cells', Plant Physiology, 136 3968-3978 (2004) [C1]
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| 2004 |
Sheahan MB, Rose RJ, McCurdy DW, 'Organelle inheritance in plant cell division: the actin cytoskeleton is required for unbiased inheritance of chloroplasts, mitochondria and endoplasmic reticulum in dividing protoplasts', Plant Journal, 37 379-390 (2004) [C1]
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| 2003 |
Offler CE, McCurdy DW, Patrick JW, Talbot MJ, 'Transfer Cells: Cells Specialized for a Special Purpose', Annual Review of Plant Biology, 54 431-454 (2003) [C1]
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| 2002 |
Baluska F, Hlavacka A, Samaj J, Palme K, Robinson D, Matoh T, et al., 'F-actin-dependent endocytosis of cell wall pectins in meristematic root cells. Insights from Brefeldin A-induced compartments', Plant Physiology, 130 422-431 (2002) [C1]
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| 2002 |
Talbot MJ, Offler CE, McCurdy DW, 'Transfer cell wall architecture: a contribution towards understanding localized wall deposition', Protoplasma, 219 197-209 (2002) [C1]
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| 2001 |
Kovar DR, Gibbon BC, McCurdy DW, Staiger CJ, 'Fluorescently-labeled fimbrin decorates a dynamic actin filament network in live plant cells', Planta, 213 390-395 (2001) [C1]
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| 2001 |
McCurdy DW, Kovar DR, Staiger CJ, 'Actin and actin-binding proteins in higher plants', Protoplasma, 215 89-104 (2001) [C1]
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| 2001 |
Talbot MJ, Franceschi VR, McCurdy DW, Offler CE, 'Wall ingrowth architecture in epidermal transfer cells of Vicia faba cotyledons', Protoplasma, 215 191-203 (2001) [C1]
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| 2000 |
Gear ML, McPhillips ML, Patrick JW, McCurdy DW, 'Hexose transporters of tomato:molecular cloning, expression analysis and functional characterization', PLANT MOLECULAR BIOLOGY, 44 687-697 (2000) [C1]
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| 2000 |
Kovar DR, Staiger CJ, Weaver EA, McCurdy DW, 'AtFim1 is an actin filament crosslinking protein from Arabidopsis thaliana', THE PLANT JOURNAL, 24 (5) 625-636 (2000) [C1]
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| 1999 |
McCurdy DW, 'Is 2,3-butanedione monoxime an effective inhibitor of myosin-based activities in plant cells?', Protoplasma, 209 120-125 (1999) [C1]
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| 1998 |
McCurdy DW, Kim S-Y, 'Molecular cloning of a novel fimbrin-like cDNA from Arabidopsis thaliana', Plant Molecular Biology, 36 23-31 (1998) [C1]
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| 1998 |
Bulbert MW, Offler CE, McCurdy DW, 'Polarized microtubule deposition coincides with wall ingrowth formation in transfer cells of Vicia faba L. cotyledons', Protoplasma, 201 8-16 (1998) [C1]
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| 1992 |
MCCURDY DW, HARMON AC, 'CALCIUM-DEPENDENT PROTEIN-KINASE IN THE GREEN-ALGA CHARA', PLANTA, 188 54-61 (1992)
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| 1992 |
McCurdy DW, Harmon AC, 'Calcium-dependent protein kinase in the green alga Chara', Planta, 188 54-61 (1992)
Cytoplasmic streaming in the characean algae is inhibited by micromolar rises in the level of cytosolic free Ca2+, but both the mechanism of action and the molecular components in... [more] Cytoplasmic streaming in the characean algae is inhibited by micromolar rises in the level of cytosolic free Ca2+, but both the mechanism of action and the molecular components involved in this process are unknown. We have used monoclonal antibodies against soybean Ca2+-dependent protein kinase (CDPK), a kinase that is activated by micromolar Ca2+ and co-localizes with actin filaments in higher-plant cells (Putnam-Evans et al., 1989, Cell Motil. Cytoskel. 12, 12-22) to identify and localize its characean homologue. Immunoblot analysis revealed that CDPK in Chara corralina Klein ex. Wild shares the same relative molecular mass (51-55 kDa) as the kinase purified from soybean, and after electrophoresis in denaturing gels is capable of phosphorylating histone III-S in a Ca2+-dependent manner. Immunofluorescence microscopy localized CDPK in Chara to the subcortical actin bundles and the surface of small organelles and other membrane components of the streaming endoplasm. The endoplasmic sites carrying CDPK were extracted from internodal cells by vacuolar perfusion with 1 mM ATP or 10-4 M Ca2+. Both the localization of CDPK and its extraction from internodal cells by perfusion with ATP or high Ca2+ are properties similar to that reported for the heavy chain of myosin in Chara (Grolig et al., 1988, Eur. J. Cell Biol. 47, 22-31). Based on its endoplasmic location and inferred enzymatic properties, we suggest that CDPK may be a putative element of the signal-transduction pathway that mediates the rapid Ca2+-induced inhibition of streaming that occurs in the characean algae. © 1992 Springer-Verlag.
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| 1992 |
MCCURDY DW, HARMON AC, 'PHOSPHORYLATION OF A PUTATIVE MYOSIN LIGHT CHAIN IN CHARA BY CALCIUM-DEPENDENT PROTEIN-KINASE', PROTOPLASMA, 171 85-88 (1992)
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| 1992 |
HARPER JDI, MCCURDY DW, SANDERS MA, SALISBURY JL, JOHN PCL, 'ACTIN DYNAMICS DURING THE CELL-CYCLE IN CHLAMYDOMONAS-REINHARDTII', CELL MOTILITY AND THE CYTOSKELETON, 22 117-126 (1992)
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| 1991 |
MCCURDY DW, PALEVITZ BA, GUNNING BES, 'EFFECT OF CYTOCHALASINS ON ACTIN IN DIVIDING ROOT-TIP CELLS OF ALLIUM AND TRITICUM - A COMPARATIVE IMMUNOCYTOCHEMICAL STUDY', CELL MOTILITY AND THE CYTOSKELETON, 18 107-112 (1991)
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| 1990 |
JOHN PCL, SEK FJ, CARMICHAEL JP, MCCURDY DW, 'P34CDC2 HOMOLOG LEVEL, CELL-DIVISION, PHYTOHORMONE RESPONSIVENESS AND CELL-DIFFERENTIATION IN WHEAT LEAVES', JOURNAL OF CELL SCIENCE, 97 627-630 (1990)
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| 1990 |
John PCL, Sek FJ, Carmichael JP, McCurdy DW, 'p34(cdc2) homologue level, cell division, phytohormone responsiveness and cell differentiation in wheat leaves', Journal of Cell Science, 97 627-630 (1990)
Formation of a plant involves generation of new cells by the division cycle and development in these of specialised structure and metabolism. Specialisation is accompanied by a de... [more] Formation of a plant involves generation of new cells by the division cycle and development in these of specialised structure and metabolism. Specialisation is accompanied by a decreasing capacity for division, which declines with particular rapidity in cells of monocotyledonous plants such as the cereals. Here we report that in wheat leaves a homologue of the cell cycle control protein p34(cdc2) participates in the control of these developmental programmes. Accumulation of p34(cdc2) to a maximum level in dividing cells and the cessation of its accumulation during subsequent cell growth and expansion indicate that it contributes specifically to division. There is a decline in p34(cdc2) level as cell differentiation proceeds, in close parallel with the previously established decline of cell division in response to auxin hormones. A basal level of p34(cdc2) in fully differentiated cells that is one-sixteenth of that in dividing cells correlates with their loss of capacity to divide. We conclude that p34(cdc2) level is controlled in diverse multicellular eukaryotes and suggest that it is an important element in the switch from cell division to differentiation.
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| 1990 |
MCCURDY DW, GUNNING BES, 'REORGANIZATION OF CORTICAL ACTIN MICROFILAMENTS AND MICROTUBULES AT PREPROPHASE AND MITOSIS IN WHEAT ROOT-TIP CELLS - A DOUBLE LABEL IMMUNOFLUORESCENCE STUDY', CELL MOTILITY AND THE CYTOSKELETON, 15 76-87 (1990)
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| 1988 |
MCCURDY DW, SAMMUT M, GUNNING BES, 'IMMUNOFLUORESCENT VISUALIZATION OF ARRAYS OF TRANSVERSE CORTICAL ACTIN MICROFILAMENTS IN WHEAT ROOT-TIP CELLS', PROTOPLASMA, 147 204-206 (1988)
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| 1987 |
MCCURDY DW, WILLIAMSON RE, 'AN ACTIN-RELATED PROTEIN INSIDE PEA-CHLOROPLASTS', JOURNAL OF CELL SCIENCE, 87 449-456 (1987)
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| 1987 |
WILLIAMSON RE, MCCURDY DW, HURLEY UA, PERKIN JL, 'ACTIN OF CHARA GIANT INTERNODAL CELLS - A SINGLE ISOFORM IN THE SUBCORTICAL FILAMENT BUNDLES AND A LARGER, IMMUNOLOGICALLY RELATED PROTEIN IN THE CHLOROPLASTS', PLANT PHYSIOLOGY, 85 268-272 (1987)
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| 1986 |
MCCURDY DW, PRATT LH, 'IMMUNOGOLD ELECTRON-MICROSCOPY OF PHYTOCHROME IN AVENA - IDENTIFICATION OF INTRACELLULAR SITES RESPONSIBLE FOR PHYTOCHROME SEQUESTERING AND ENHANCED PELLETABILITY', JOURNAL OF CELL BIOLOGY, 103 2541-2550 (1986)
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| 1986 |
MCCURDY DW, PRATT LH, 'KINETICS OF INTRACELLULAR REDISTRIBUTION OF PHYTOCHROME IN AVENA COLEOPTILES AFTER ITS PHOTOCONVERSION TO THE ACTIVE, FAR-RED-ABSORBING FORM', PLANTA, 167 330-336 (1986)
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| 1986 |
WILLIAMSON RE, PERKIN JL, MCCURDY DW, CRAIG S, HURLEY UA, 'PRODUCTION AND USE OF MONOCLONAL-ANTIBODIES TO STUDY THE CYTOSKELETON AND OTHER COMPONENTS OF THE CORTICAL CYTOPLASM OF CHARA', EUROPEAN JOURNAL OF CELL BIOLOGY, 41 1-8 (1986)
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