Dr Elizabeth Bromfield
Honorary Senior Lecturer
School of Environmental and Life Sciences
- Email:elizabeth.bromfield@newcastle.edu.au
- Phone:(02) 492 16267
Career Summary
Biography
I am an ARC DECRA Fellow in the Priority Research Centre for Reproductive Science. My research is primarily focused on discovering how human sperm recognise and interact with the egg to improve in vitro fertilisation outcomes and ensure the health of new individuals.
I completed my PhD under the supervision of Prof. Brett Nixon and L. Prof. John Aitken at The University of Newcastle and was awarded my degree in December, 2015. My doctoral study was dedicated to understanding the mechanisms behind human sperm-egg recognition with a particular focus on investigating the molecules that jeopardise this process in cases of infertility. I have studied this complex biological interaction between the male and female gametes by evaluating the formation and function of important protein complexes at the sperm surface that act as mediators in the interaction between sperm and the egg. Importantly, working with members of the PRC in Reproductive Science, we have discovered a very deleterious role for reactive oxygen species in protein complex function and, as a consequence, we have demonstrated that oxidative stress is a major detriment to fertilisation outcomes in our own species.
In addition to clarifying the aetiology of male infertility, this work has assisted in the identification and characterisation of molecules that may be used in the development of diagnostic assays for predicting the success of in vitro fertilization.
My current research areas of interest include:
1. Understanding the role of molecular chaperones in protein complex assembly in gametes
2. Investigating links between in vitro ageing, protein aggregation and molecular chaperone activity in the oocyte
3. Studying the effect of lipid aldehydes on protein quality control in germ cells
4. Examining how germ cells respond to oxidative stress and refining strategies to intervene in the production of reactive oxygen species
Qualifications
- Doctor of Philosophy, University of Newcastle
- Bachelor of Biotechnology, University of Newcastle
- Bachelor of Biotechnology (Honours), University of Newcastle
Keywords
- Cell Biology
- Fertility
- Lipids
- Molecular chaperones
- Oxidative stress
- Protein homeostasis
- Redox Biology
- Reproductive Biology
Fields of Research
Code | Description | Percentage |
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310199 | Biochemistry and cell biology not elsewhere classified | 100 |
Awards
Award
Year | Award |
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2016 |
Faculty of Science & IT Award for Research Excellence Faculty of Science and Information Technology,The University of Newcastle |
2014 |
Best Oral Presentation Australian Society for Biochemistry and Molecular Biology |
2013 |
Best poster award Australian Society for Biochemistry and Molecular Biology |
Prize
Year | Award |
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2020 |
Reproduction Emerging Leader Award from the Society of Reproductive Biology Society for Reproductive Biology |
2016 |
Best poster prize Australian Society for Medical Research (ASMR) |
2015 |
Best Overall Oral Presentation Australian Society for Medical Research (ASMR) |
Scholarship
Year | Award |
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2014 |
Travel grant to attend WCRB2014 Society for Reproductive Biology |
Teaching
Code | Course | Role | Duration |
---|---|---|---|
Biol2001 |
Laboratory Skills in Biological Sciences The University of Newcastle |
Demonstrator | 21/2/2011 - 10/7/2015 |
Biol2002 |
Laboratory Skills for Biological Sciences The University of Newcastle |
Lecturer | 27/7/2015 - 27/11/2015 |
Biol3020 |
Reproductive Physiology Univeristy of Newcastle |
Lecturer | 27/7/2015 - 27/11/2015 |
Publications
For publications that are currently unpublished or in-press, details are shown in italics.
Chapter (5 outputs)
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2021 |
Schultz B, Hulse L, Nicolson V, Larkin R, Bromfield E, Nixon B, Johnston S, 'Prolonged Chilled Preservation and Preliminary Investigations of Energy Production of Koala (Phascolarctos cinereus) Spermatozoa', XIIIth International Symposium on Spermatology, Springer International Publishing 277-278 (2021)
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2021 |
Nixon B, Cafe SL, Bromfield EG, De Iuliis G, Dun M, 'Capacitation and Acrosome Reaction: Histochemical Techniques to Determine Acrosome Reaction', Manual of Sperm Function Testing in Human Assisted Reproduction, Cambridge University Press, Cambridge 81-92 (2021)
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2020 |
Nixon B, Bromfield E, 'New Horizons in Male Subfertility and Infertility', Male and Sperm Factors that Maximize IVF Success, Cambridge University Press, Cambridge, UK (2020)
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2017 |
Nixon B, Bromfield EG, Cui J, De Iuliis GN, 'Heat shock protein A2 (HSPA2): regulatory roles in germ cell development and sperm', The Role of Heat Shock Proteins in Reproductive System Development and Function, Springer International Publishing, Cham, Switzerland 67-93 (2017) [B1]
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Journal article (59 outputs)
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2024 |
Smyth SP, Nixon B, Skerrett-Byrne DA, Burke ND, Bromfield EG, 'Building an Understanding of Proteostasis in Reproductive Cells: The Impact of Reactive Carbonyl Species on Protein Fate.', Antioxid Redox Signal, (2024) [C1]
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2023 |
Zhang M, Chiozzi RZ, Bromfield EG, Heck AJ, Helms JB, Gadella BM, 'Characterization of acrosin and acrosin binding protein as novel CRISP2 interacting proteins in boar spermatozoa.', Andrology, 11 1460-1471 (2023) [C1]
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2023 |
Perera TRW, Gibb Z, Bromfield EG, Rupasinghe T, Skerrett-Byrne DA, Swegen A, 'Early pregnancy associated changes in systemic lipid profiles of mares revealed by high resolution mass spectrometry', Journal of Equine Veterinary Science, 125 104780-104780 (2023) [C1]
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2023 |
Nixon B, Schjenken JE, Burke ND, Skerrett-Byrne DA, Hart HM, De Iuliis GN, et al., 'New horizons in human sperm selection for assisted reproduction', Frontiers in Endocrinology, 14 (2023) [C1] Male infertility is a commonly encountered pathology that is estimated to be a contributory factor in approximately 50% of couples seeking recourse to assisted reproductive techno... [more] Male infertility is a commonly encountered pathology that is estimated to be a contributory factor in approximately 50% of couples seeking recourse to assisted reproductive technologies. Upon clinical presentation, such males are commonly subjected to conventional diagnostic andrological practices that rely on descriptive criteria to define their fertility based on the number of morphologically normal, motile spermatozoa encountered within their ejaculate. Despite the virtual ubiquitous adoption of such diagnostic practices, they are not without their limitations and accordingly, there is now increasing awareness of the importance of assessing sperm quality in order to more accurately predict a male¿s fertility status. This realization raises the important question of which characteristics signify a high-quality, fertilization competent sperm cell. In this review, we reflect on recent advances in our mechanistic understanding of sperm biology and function, which are contributing to a growing armory of innovative approaches to diagnose and treat male infertility. In particular we review progress toward the implementation of precision medicine; the robust clinical adoption of which in the setting of fertility, currently lags well behind that of other fields of medicine. Despite this, research shows that the application of advanced technology platforms such as whole exome sequencing and proteomic analyses hold considerable promise in optimizing outcomes for the management of male infertility by uncovering and expanding our inventory of candidate infertility biomarkers, as well as those associated with recurrent pregnancy loss. Similarly, the development of advanced imaging technologies in tandem with machine learning artificial intelligence are poised to disrupt the fertility care paradigm by advancing our understanding of the molecular and biological causes of infertility to provide novel avenues for future diagnostics and treatments.
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2022 |
Zhang M, Chiozzi RZ, Skerrett-Byrne DA, Veenendaal T, Klumperman J, Heck AJR, et al., 'High Resolution Proteomic Analysis of Subcellular Fractionated Boar Spermatozoa Provides Comprehensive Insights Into Perinuclear Theca-Residing Proteins.', Front Cell Dev Biol, 10 836208 (2022) [C1]
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2022 |
Aitken RJ, Bromfield E, Gibb Z, 'The impact of oxidative stress on reproduction: a focus on gametogenesis and fertilization.', Reproduction, 164 F79-F94 (2022) [C1]
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2022 |
Maitan P, Bromfield EG, Stout TAE, Gadella BM, Leemans B, 'A stallion spermatozoon's journey through the mare's genital tract: In vivo and in vitro aspects of sperm capacitation', Animal Reproduction Science, 246 (2022) [C1] Conventional in vitro fertilization is not efficacious when working with equine gametes. Although stallion spermatozoa bind to the zona pellucida in vitro, these gametes fail to i... [more] Conventional in vitro fertilization is not efficacious when working with equine gametes. Although stallion spermatozoa bind to the zona pellucida in vitro, these gametes fail to initiate the acrosome reaction in the vicinity of the oocyte and cannot, therefore, penetrate into the perivitelline space. Failure of sperm penetration most likely relates to the absence of optimized in vitro fertilization media containing molecules essential to support stallion sperm capacitation. In vivo, the female reproductive tract, especially the oviductal lumen, provides an environmental milieu that appropriately regulates interactions between the gametes and promotes fertilization. Identifying these ¿fertilization supporting factors¿ would be a great contribution for development of equine in vitro fertilization media. In this review, a description of the current understanding of the interactions stallion spermatozoa undergo during passage through the female genital tract, and related specific molecular changes that occur at the sperm plasma membrane is provided. Understanding these molecular changes may hold essential clues to achieving successful in vitro fertilization with equine gametes.
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2022 |
Martin JH, Nixon B, Cafe SL, Aitken RJ, Bromfield EG, Lord T, 'OXIDATIVE STRESS AND REPRODUCTIVE FUNCTION: Oxidative stress and
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2022 |
Burke ND, Nixon B, Roman SD, Schjenken JE, Walters JLH, Aitken RJ, Bromfield EG, 'Male infertility and somatic health - insights into lipid damage as a mechanistic link', NATURE REVIEWS UROLOGY, 19 727-750 (2022) [C1]
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2022 |
Skerrett-Byrne DA, Anderson AL, Bromfield EG, Bernstein IR, Mulhall JE, Schjenken JE, et al., 'Global profiling of the proteomic changes associated with the post-testicular maturation of mouse spermatozoa', Cell Reports, 41 (2022) [C1] Spermatozoa acquire fertilization potential during passage through a highly specialized region of the extratesticular ductal system known as the epididymis. In the absence of de n... [more] Spermatozoa acquire fertilization potential during passage through a highly specialized region of the extratesticular ductal system known as the epididymis. In the absence of de novo gene transcription or protein translation, this functional transformation is extrinsically driven via the exchange of varied macromolecular cargo between spermatozoa and the surrounding luminal plasma. Key among these changes is a substantive remodeling of the sperm proteomic architecture, the scale of which has yet to be fully resolved. Here, we have exploited quantitative mass spectrometry-based proteomics to define the extent of changes associated with the maturation of mouse spermatozoa; reporting the identity of >6,000 proteins, encompassing the selective loss and gain of several hundred proteins. Further, we demonstrate epididymal-driven activation of RHOA-mediated signaling pathways is an important component of sperm maturation. These data contribute molecular insights into the complexity of proteomic changes associated with epididymal sperm maturation.
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2022 |
Zhang M, Bromfield EG, Helms JB, Gadella BM, 'The fate of porcine sperm CRISP2 from the perinuclear theca before and after in vitro fertilization
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2022 |
Leemans B, Bromfield EG, Stout TAE, Vos M, Van der Ham H, Van Beek R, et al., 'Developing a reproducible protocol for culturing functional confluent monolayers of differentiated equine oviduct epithelial cells(dagger)', BIOLOGY OF REPRODUCTION, 106 710-729 (2022) [C1]
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2021 |
Zhang M, Bromfield EG, Veenendaal T, Klumperman J, Helms JB, Gadella BM, 'Characterization of different oligomeric forms of CRISP2 in the perinuclear theca versus the fibrous tail structures of boar spermatozoa', BIOLOGY OF REPRODUCTION, 105 1160-1170 (2021) [C1]
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2021 |
Nixon B, Anderson AL, Bromfield EG, Martin JH, Cafe SL, Skerrett-Byrne DA, et al., 'Post-testicular sperm maturation in the saltwater crocodile Crocodylus porosus: assessing the temporal acquisition of sperm motility', REPRODUCTION FERTILITY AND DEVELOPMENT, 33 530-539 (2021) [C1]
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2021 |
Cafe SL, Nixon B, Ecroyd H, Martin JH, Skerrett-Byrne DA, Bromfield EG, 'Proteostasis in the Male and Female Germline: A New Outlook on the Maintenance of Reproductive Health', FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY, 9 (2021) [C1]
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2021 |
Skerrett-Byrne DA, Bromfield EG, Murray HC, Jamaluddin MFB, Jarnicki AG, Fricker M, et al., 'Time-resolved proteomic profiling of cigarette smoke-induced experimental chronic obstructive pulmonary disease', Respirology, 26 960-973 (2021) [C1] Background and objective: Chronic obstructive pulmonary disease (COPD) is the third leading cause of illness and death worldwide. Current treatments aim to control symptoms with n... [more] Background and objective: Chronic obstructive pulmonary disease (COPD) is the third leading cause of illness and death worldwide. Current treatments aim to control symptoms with none able to reverse disease or stop its progression. We explored the major molecular changes in COPD pathogenesis. Methods: We employed quantitative label-based proteomics to map the changes in the lung tissue proteome of cigarette smoke-induced experimental COPD that is induced over 8 weeks and progresses over 12 weeks. Results: Quantification of 7324 proteins enabled the tracking of changes to the proteome. Alterations in protein expression profiles occurred in the induction phase, with 18 and 16 protein changes at 4- and 6-week time points, compared to age-matched controls, respectively. Strikingly, 269 proteins had altered expression after 8 weeks when the hallmark pathological features of human COPD emerge, but this dropped to 27 changes at 12 weeks with disease progression. Differentially expressed proteins were validated using other mouse and human COPD bronchial biopsy samples. Major changes in RNA biosynthesis (heterogeneous nuclear ribonucleoproteins C1/C2 [HNRNPC] and RNA-binding protein Musashi homologue 2 [MSI2]) and modulators of inflammatory responses (S100A1) were notable. Mitochondrial dysfunction and changes in oxidative stress proteins also occurred. Conclusion: We provide a detailed proteomic profile, identifying proteins associated with the pathogenesis and disease progression of COPD establishing a platform to develop effective new treatment strategies.
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2021 |
Skerrett-Byrne DA, Trigg NA, Bromfield EG, Dun MD, Bernstein IR, Anderson AL, et al., 'Proteomic dissection of the impact of environmental exposures on mouse seminal vesicle function', Molecular and Cellular Proteomics, 20 (2021) [C1] Seminal vesicles are an integral part of the male reproductive accessory gland system. They produce a complex array of secretions containing bioactive constituents that support ga... [more] Seminal vesicles are an integral part of the male reproductive accessory gland system. They produce a complex array of secretions containing bioactive constituents that support gamete function and promote reproductive success, with emerging evidence suggesting these secretions are influenced by our environment. Despite their significance, the biology of seminal vesicles remains poorly defined. Here, we complete the first proteomic assessment of mouse seminal vesicles and assess the impact of the reproductive toxicant acrylamide. Mice were administered acrylamide (25 mg/kg bw/day) or control daily for five consecutive days prior to collecting seminal vesicle tissue. A total of 5013 proteins were identified in the seminal vesicle proteome with bioinformatic analyses identifying cell proliferation, protein synthesis, cellular death, and survival pathways as prominent biological processes. Secreted proteins were among the most abundant, and several proteins are linked with seminal vesicle phenotypes. Analysis of the effect of acrylamide on the seminal vesicle proteome revealed 311 differentially regulated (FC ± 1.5, p = 0.05, 205 up-regulated, 106 downregulated) proteins, orthogonally validated via immunoblotting and immunohistochemistry. Pathways that initiate protein synthesis to promote cellular survival were prominent among the dysregulated pathways, and rapamycin-insensitive companion of mTOR (RICTOR, p = 6.69E-07) was a top-ranked upstream driver. Oxidative stress was implicated as contributing to protein changes, with acrylamide causing an increase in 8-OHdG in seminal vesicle epithelial cells (fivefold increase, p = 0.016) and the surrounding smooth muscle layer (twofold increase, p = 0.043). Additionally, acrylamide treatment caused a reduction in seminal vesicle secretion weight (36% reduction, p = 0.009) and total protein content (25% reduction, p = 0.017). Together these findings support the interpretation that toxicant exposure influences male accessory gland physiology and highlights the need to consider the response of all male reproductive tract tissues when interpreting the impact of environmental stressors on male reproductive function.
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2021 |
Skerrett-Byrne DA, Anderson AL, Hulse L, Wass C, Dun MD, Bromfield EG, et al., 'Proteomic analysis of koala (phascolarctos cinereus) spermatozoa and prostatic bodies', Proteomics, 21 (2021) [C1] The aims of this study were to investigate the proteome of koala spermatozoa and that of the prostatic bodies with which they interact during ejaculation. For this purpose, sperma... [more] The aims of this study were to investigate the proteome of koala spermatozoa and that of the prostatic bodies with which they interact during ejaculation. For this purpose, spermatozoa and prostatic bodies were fractionated from the semen of four male koalas and analysed by HPLC MS/MS. This strategy identified 744 sperm and 1297 prostatic body proteins, which were subsequently attributed to 482 and 776 unique gene products, respectively. Gene ontology curation of the sperm proteome revealed an abundance of proteins mapping to the canonical sirtuin and 14-3-3 signalling pathways. By contrast, protein ubiquitination and unfolded protein response pathways dominated the equivalent analysis of proteins uniquely identified in prostatic bodies. Koala sperm proteins featured an enrichment of those mapping to the functional categories of cellular compromise/inflammatory response, whilst those of the prostatic body revealed an over-representation of molecular chaperone and stress-related proteins. Cross-species comparisons demonstrated that the koala sperm proteome displays greater conservation with that of eutherians (human; 93%) as opposed to reptile (crocodile; 39%) and avian (rooster; 27%) spermatozoa. Together, this work contributes to our overall understanding of the core sperm proteome and has identified biomarkers that may contribute to the exceptional longevity of koala spermatozoa during ex vivo storage.
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2021 |
Walters JLH, Anderson AL, da Silva SJM, Aitken RJ, De Iuliis GN, Sutherland JM, et al., 'Mechanistic insight into the regulation of lipoxygenase-driven lipid peroxidation events in human spermatozoa and their impact on male fertility', Antioxidants, 10 1-19 (2021) [C1] A prevalent cause of sperm dysfunction in male infertility patients is the overproduction of reactive oxygen species, an attendant increase in lipid peroxidation and the productio... [more] A prevalent cause of sperm dysfunction in male infertility patients is the overproduction of reactive oxygen species, an attendant increase in lipid peroxidation and the production of cyto-toxic reactive carbonyl species such as 4-hydroxynonenal. Our previous studies have implicated arachidonate 15-lipoxygenase (ALOX15) in the production of 4-hydroxynonenal in developing germ cells. Here, we have aimed to develop a further mechanistic understanding of the lipoxygen-ase-lipid peroxidation pathway in human spermatozoa. Through pharmacological inhibition studies, we identified a protective role for phospholipase enzymes in the liberation of peroxidised polyunsaturated fatty acids from the human sperm membrane. Our results also revealed that arachi-donic acid, linoleic acid and docosahexanoic acid are key polyunsaturated fatty acid substrates for ALOX15. Upon examination of ALOX15 in the spermatozoa of infertile patients compared to their normozoospermic counterparts, we observed significantly elevated levels of ALOX15 protein abundance in the infertile population and an increase in 4-hydroxynonenal adducts. Collectively, these data confirm the involvement of ALOX15 in the oxidative stress cascade of human spermatozoa and support the notion that increased ALOX15 abundance in sperm cells may accentuate membrane lipid peroxidation and cellular dysfunction, ultimately contributing to male infertility.
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2021 |
Skerrett-Byrne DA, Nixon B, Bromfield EG, Breen J, Trigg NA, Stanger SJ, et al., 'Transcriptomic analysis of the seminal vesicle response to the reproductive toxicant acrylamide', BMC Genomics, 22 (2021) [C1] Background: The seminal vesicles synthesise bioactive factors that support gamete function, modulate the female reproductive tract to promote implantation, and influence developme... [more] Background: The seminal vesicles synthesise bioactive factors that support gamete function, modulate the female reproductive tract to promote implantation, and influence developmental programming of offspring phenotype. Despite the significance of the seminal vesicles in reproduction, their biology remains poorly defined. Here, to advance understanding of seminal vesicle biology, we analyse the mouse seminal vesicle transcriptome under normal physiological conditions and in response to acute exposure to the reproductive toxicant acrylamide. Mice were administered acrylamide (25 mg/kg bw/day) or vehicle control daily for five consecutive days prior to collecting seminal vesicle tissue 72 h following the final injection. Results: A total of 15,304 genes were identified in the seminal vesicles with those encoding secreted proteins amongst the most abundant. In addition to reproductive hormone pathways, functional annotation of the seminal vesicle transcriptome identified cell proliferation, protein synthesis, and cellular death and survival pathways as prominent biological processes. Administration of acrylamide elicited 70 differentially regulated (fold-change =1.5 or = 0.67) genes, several of which were orthogonally validated using quantitative PCR. Pathways that initiate gene and protein synthesis to promote cellular survival were prominent amongst the dysregulated pathways. Inflammation was also a key transcriptomic response to acrylamide, with the cytokine, Colony stimulating factor 2 (Csf2) identified as a top-ranked upstream driver and inflammatory mediator associated with recovery of homeostasis. Early growth response (Egr1), C-C motif chemokine ligand 8 (Ccl8), and Collagen, type V, alpha 1 (Col5a1) were also identified amongst the dysregulated genes. Additionally, acrylamide treatment led to subtle changes in the expression of genes that encode proteins secreted by the seminal vesicle, including the complement regulator, Complement factor b (Cfb). Conclusions: These data add to emerging evidence demonstrating that the seminal vesicles, like other male reproductive tract tissues, are sensitive to environmental insults, and respond in a manner with potential to exert impact on fetal development and later offspring health.
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2021 |
Peters AE, Caban SJ, McLaughlin EA, Roman SD, Bromfield EG, Nixon B, Sutherland JM, 'The Impact of Aging on Macroautophagy in the Pre-ovulatory Mouse Oocyte', Frontiers in Cell and Developmental Biology, 9 (2021) [C1] Accompanying the precipitous age-related decline in human female fertility is an increase in the proportion of poor-quality oocytes within the ovary. The macroautophagy pathway, a... [more] Accompanying the precipitous age-related decline in human female fertility is an increase in the proportion of poor-quality oocytes within the ovary. The macroautophagy pathway, an essential protein degradation mechanism responsible for maintaining cell health, has not yet been thoroughly investigated in this phenomenon. The aim of this study was to characterize the macroautophagy pathway in an established mouse model of oocyte aging using in-depth image analysis-based methods and to determine mechanisms that account for the observed changes. Three autophagy pathway markers were selected for assessment of gene and protein expression in this model: Beclin 1; an initiator of autophagosome formation, Microtubule-associated protein 1 light chain 3B; a constituent of the autophagosome membrane, and lysosomal-associated membrane protein 1; a constituent of the lysosome membrane. Through quantitative image analysis of immunolabeled oocytes, this study revealed impairment of the macroautophagy pathway in the aged oocyte with an attenuation of both autophagosome and lysosome number. Additionally, an accumulation of amphisomes greater than 10 µm2 in area were observed in aging oocytes, and this accumulation was mimicked in oocytes treated with lysosomal inhibitor chloroquine. Overall, these findings implicate lysosomal dysfunction as a prominent mechanism by which these age-related changes may occur and highlight the importance of macroautophagy in maintaining mouse pre-ovulatory oocyte quality. This provides a basis for further investigation of dysfunctional autophagy in poor oocyte quality and for the development of therapeutic or preventative strategies to aid in the maintenance of pre-ovulatory oocyte health.
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2021 |
Leung MR, Chiozzi RZ, Roelofs MC, Hevler JF, Ravi RT, Maitan P, et al., 'In-cell structures of conserved supramolecular protein arrays at the mitochondria-cytoskeleton interface in mammalian sperm', PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 118 (2021) [C1]
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2021 |
Tamessar CT, Trigg NA, Nixon B, Skerrett-Byrne DA, Sharkey DJ, Robertson SA, et al., 'Roles of male reproductive tract extracellular vesicles in reproduction', AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY, 85 (2021) [C1]
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2021 |
Leung MR, Roelofs MC, Ravi RT, Maitan P, Henning H, Zhang M, et al., 'The multi-scale architecture of mammalian sperm flagella and implications for ciliary motility', EMBO JOURNAL, 40 (2021) [C1]
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2021 |
Nixon B, Anderson AL, Bromfield EG, Martin JH, Lord T, Cafe SL, et al., 'Gross and microanatomy of the male reproductive duct system of the saltwater crocodile Crocodylus porosus', REPRODUCTION FERTILITY AND DEVELOPMENT, 33 540-554 (2021) [C1]
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2021 |
Trigg NA, Stanger SJ, Zhou W, Skerrett-Byrne DA, Sipilä P, Dun MD, et al., 'A novel role for milk fat globule-EGF factor 8 protein (MFGE8) in the mediation of mouse sperm extracellular vesicle interactions', Proteomics, 21 (2021) [C1] Spermatozoa transition to functional maturity as they are conveyed through the epididymis, a highly specialized region of the male excurrent duct system. Owing to their transcript... [more] Spermatozoa transition to functional maturity as they are conveyed through the epididymis, a highly specialized region of the male excurrent duct system. Owing to their transcriptionally and translationally inert state, this transformation into fertilization competent cells is driven by complex mechanisms of intercellular communication with the secretory epithelium that delineates the epididymal tubule. Chief among these mechanisms are the release of extracellular vesicles (EV), which have been implicated in the exchange of varied macromolecular cargo with spermatozoa. Here, we describe the optimization of a tractable cell culture model to study the mechanistic basis of sperm¿extracellular vesicle interactions. In tandem with receptor inhibition strategies, our data demonstrate the importance of milk fat globule-EGF factor 8 (MFGE8) protein in mediating the efficient exchange of macromolecular EV cargo with mouse spermatozoa; with the MFGE8 integrin-binding Arg-Gly-Asp (RGD) tripeptide motif identified as being of particular importance. Specifically, complementary strategies involving MFGE8 RGD domain ablation, competitive RGD-peptide inhibition and antibody-masking of alpha V integrin receptors, all significantly inhibited the uptake and redistribution of EV-delivered proteins into immature mouse spermatozoa. These collective data implicate the MFGE8 ligand and its cognate integrin receptor in the mediation of the EV interactions that underpin sperm maturation.
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2021 |
Maitan PP, Bromfield EG, Hoogendijk R, Leung MR, Zeev-Ben-Mordehai T, van de Lest CH, et al., 'Bicarbonate-Stimulated Membrane Reorganization in Stallion Spermatozoa', Frontiers in Cell and Developmental Biology, 9 (2021) [C1] Classical in vitro fertilization (IVF) is still poorly successful in horses. This lack of success is thought to be due primarily to inadequate capacitation of stallion spermatozoa... [more] Classical in vitro fertilization (IVF) is still poorly successful in horses. This lack of success is thought to be due primarily to inadequate capacitation of stallion spermatozoa under in vitro conditions. In species in which IVF is successful, bicarbonate, calcium, and albumin are considered the key components that enable a gradual reorganization of the sperm plasma membrane that allows the spermatozoa to undergo an acrosome reaction and fertilize the oocyte. The aim of this work was to comprehensively examine contributors to stallion sperm capacitation by investigating bicarbonate-induced membrane remodelling steps, and elucidating the contribution of cAMP signalling to these events. In the presence of capacitating media containing bicarbonate, a significant increase in plasma membrane fluidity was readily detected using merocyanine 540 staining in the majority of viable spermatozoa within 15¿min of bicarbonate exposure. Specific inhibition of soluble adenylyl cyclase (sAC) in the presence of bicarbonate by LRE1 significantly reduced the number of viable sperm with high membrane fluidity. This suggests a vital role for sAC-mediated cAMP production in the regulation of membrane fluidity. Cryo-electron tomography of viable cells with high membrane fluidity revealed a range of membrane remodelling intermediates, including destabilized membranes and zones with close apposition of the plasma membrane and the outer acrosomal membrane. However, lipidomic analysis of equivalent viable spermatozoa with high membrane fluidity demonstrated that this phenomenon was neither accompanied by a gross change in the phospholipid composition of stallion sperm membranes nor detectable sterol efflux (p > 0.05). After an early increase in membrane fluidity, a significant and cAMP-dependent increase in viable sperm with phosphatidylserine (PS), but not phosphatidylethanolamine (PE) exposure was noted. While the events observed partly resemble findings from the in vitro capacitation of sperm from other mammalian species, the lack of cholesterol removal appears to be an equine-specific phenomenon. This research will assist in the development of a defined medium for the capacitation of stallion sperm and will facilitate progress toward a functional IVF protocol for horse gametes.
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2020 |
Nixon B, Cafe SL, Eamens AL, De Iuliis GN, Bromfield EG, Martin JH, et al., 'Molecular insights into the divergence and diversity of post-testicular maturation strategies', Molecular and Cellular Endocrinology, 517 110955-110955 (2020) [C1]
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2020 |
Walters JLH, Gadella BM, Sutherland JM, Nixon B, Bromfield EG, 'Male Infertility: Shining a Light on Lipids and Lipid-Modulating Enzymes in the Male Germline', Journal of Clinical Medicine, 9 (2020) [C1]
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2020 |
Cafe SL, Nixon B, Dun MD, Roman SD, Bernstein IR, Bromfield EG, 'Oxidative Stress Dysregulates Protein Homeostasis within the Male Germ Line', Antioxidants and Redox Signaling, 32 487-503 (2020) [C1]
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2020 |
Peters AE, Mihalas BP, Bromfield EG, Roman SD, Nixon B, Sutherland JM, 'Autophagy in Female Fertility: A Role in Oxidative Stress and Aging', ANTIOXIDANTS & REDOX SIGNALING, 32 550-568 (2020) [C1]
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2020 |
Winship A, Donoghue J, Houston BJ, Martin JH, Lord T, Adwal A, et al., 'Reproductive health research in Australia and New Zealand: Highlights from the Annual Meeting of the Society for Reproductive Biology, 2019', Reproduction, Fertility and Development, 32 637-647 (2020) [C1]
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2019 |
Martin JH, Aitken RJ, Bromfield E, Cafe SL, Sutherland JM, Frost ER, et al., 'Investigation into the presence and functional significance of proinsulin C-peptide in the female germline', Biology of Reproduction, 100 1275-1289 (2019) [C1]
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2019 |
Nixon B, De Iuliis GN, Hart HM, Zhou W, Mathe A, Bernstein IR, et al., 'Proteomic profiling of mouse epididymosomes reveals their contributions to post-testicular sperm maturation', Molecular and Cellular Proteomics, 18 S91-S108 (2019) [C1]
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2019 |
Brown P, RELISH Consortium, Zhou Y, 'Large expert-curated database for benchmarking document similarity detection in biomedical literature search', Database, 2019 (2019) [C1]
|
Nova | |||||||||
2019 |
Nixon B, Johnston SD, Skerrett-Byrne DA, Anderson AL, Stanger SJ, Bromfield EG, et al., 'Modification of Crocodile Spermatozoa Refutes the Tenet That Post-testicular Sperm Maturation Is Restricted To Mammals', MOLECULAR & CELLULAR PROTEOMICS, 18 S59-S76 (2019) [C1]
|
Nova | |||||||||
2019 |
Nixon B, Bernstein IR, Cafe SL, Delehedde M, Sergeant N, Anderson AL, et al., 'A Kinase Anchor Protein 4 is vulnerable to oxidative adduction in male germ cells', Frontiers in Cell and Developmental Biology, 7 (2019) [C1]
|
Nova | |||||||||
2019 |
Bromfield E, Walters JLH, Cafe S, Bernstein I, Stanger SR, Anderson AL, et al., 'Differential cell death decisions in the testis: evidence for an exclusive window of ferroptosis in round spermatids', Molecular Human Reproduction, 25 241-256 (2019) [C1]
|
Nova | |||||||||
2019 |
Martin JH, Aitken RJ, Bromfield EG, Nixon B, 'DNA damage and repair in the female germline: contributions to ART.', Human Reproduction Update, 25 180-201 (2019) [C1]
|
Nova | |||||||||
2018 |
Martin J, Bromfield EG, Aitken RJ, Lord T, Nixon B, 'Double Strand Break DNA Repair occurs via Non-Homologous End-Joining in Mouse MII Oocytes', Scientific Reports, 8 1-15 (2018) [C1]
|
Nova | |||||||||
2018 |
Walters JLH, De Iuliis G, Nixon B, Bromfield EG, 'Oxidative Stress in the Male Germline: A Review of Novel Strategies to Reduce 4-Hydroxynonenal Production', Antioxidants, 7 (2018) [C1]
|
Nova | |||||||||
2018 |
Walters JLH, De Iuliis GN, Dun MD, Aitken RJ, McLaughlin EA, Nixon B, Bromfield EG, 'Pharmacological inhibition of arachidonate 15-lipoxygenase protects human spermatozoa against oxidative stress.', Biology of reproduction, 98 784-794 (2018) [C1]
|
Nova | |||||||||
2018 |
Mihalas BP, Bromfield EG, Sutherland JM, De Iuliis GN, McLaughlin EA, John Aitken R, Nixon B, 'Oxidative damage in naturally aged mouse oocytes is exacerbated by dysregulation of proteasomal activity', Journal of Biological Chemistry, 293 18944-18964 (2018) [C1]
|
Nova | |||||||||
2018 |
Houston BJ, Nixon B, Martin JH, De Iuliis GN, Trigg NA, Bromfield EG, et al., 'Heat exposure induces oxidative stress and DNA damage in the male germ line', BIOLOGY OF REPRODUCTION, 98 593-606 (2018) [C1]
|
Nova | |||||||||
2017 |
Bromfield EG, Aitken RJ, McLaughlin EA, Nixon B, 'Proteolytic degradation of heat shock protein A2 occurs in response to oxidative stress in male germ cells of the mouse', MOLECULAR HUMAN REPRODUCTION, 23 91-105 (2017) [C1]
|
Nova | |||||||||
2017 |
Bromfield EG, Mihalas BP, Dun MD, Aitken RJ, McLaughlin EA, Walters JLH, Nixon B, 'Inhibition of arachidonate 15-lipoxygenase prevents 4-hydroxynonenal-induced proteindamage in male germ cells', Biology of Reproduction, 96 598-609 (2017) [C1]
|
Nova | |||||||||
2017 |
Martin JH, Bromfield EG, Aitken RJ, Nixon B, 'Biochemical alterations in the oocyte in support of early embryonic development', CELLULAR AND MOLECULAR LIFE SCIENCES, 74 469-485 (2017) [C1]
|
Nova | |||||||||
2016 |
Bromfield EG, McLaughlin EA, Aitken RJ, Nixon B, 'Heat shock protein member A2 forms a stable complex with angiotensin convertingenzymeand protein disulfide isomerase A6 in human spermatozoa', Molecular Human Reproduction, 22 93-109 (2016) [C1]
|
Nova | |||||||||
2016 |
Martin JH, Nixon B, Lord T, Bromfield EG, Aitken RJ, 'Identification of a key role for permeability glycoprotein in enhancing the cellular defense mechanisms of fertilized oocytes', DEVELOPMENTAL BIOLOGY, 417 63-76 (2016) [C1]
|
Nova | |||||||||
2015 |
Bromfield E, Aitken RJ, Nixon B, 'Novel characterization of the HSPA2-stabilizing protein BAG6 in human spermatozoa', Molecular Human Reproduction, 21 755-769 (2015) [C1] While a large cohort of sperm surface receptors underpin sperm-oocyte adhesion processes, our recent work has revealed that the molecular chaperone Heat Shock Protein A2 (HSPA2) i... [more] While a large cohort of sperm surface receptors underpin sperm-oocyte adhesion processes, our recent work has revealed that the molecular chaperone Heat Shock Protein A2 (HSPA2) is a key regulator of zona pellucida-receptor complex assembly in our own species. Indeed, in the infertile population, spermatozoa that fail to interact with the zona pellucida of the oocyte consistently lack HSPA2 protein expression. While the mechanisms behind this protein deficiency are under consideration, BCL2-associated athanogene 6 (BAG6) has been identified as a key regulator of HSPA2 stability in mouse germ cells. However, in the human, the presence of BAG family proteins remains completely uncharacterized. Consequently, this study aimed to determine the presence of BAG6 in human sperm cells and to characterize its putative interaction with HSPA2 throughout sperm cell development. BAG6 was shown to co-localize with HSPA2 in human testicular germ cells and epididymal spermatozoa. Similarly, BAG6 was identified in the equatorial region of non-capacitated spermatozoa but underwent a marked relocation to the anterior region of the head upon the induction of capacitation in these cells. Protein-protein interaction assays revealed the stable interaction of BAG6 and HSPA2 proteins in mature spermatozoa. Furthermore, examination of the spermatozoa of infertile men with zona pellucida binding defects, related to a lack of HSPA2, revealed a concomitant deficiency in BAG6 protein expression. In view of the findings described in this study, we propose that BAG6 is likely a key regulator of HSPA2 stability/function in human germ cells. Moreover, its under-representation in spermatozoa with zona pellucida binding deficiency suggests that BAG6 may be an important candidate to study for a further understanding of male idiopathic infertility.
|
Nova | |||||||||
2015 |
Bromfield EG, Aitken RJ, Anderson AL, McLaughlin EA, Nixon B, 'The impact of oxidative stress on chaperone-mediated human sperm-egg interaction.', Hum Reprod, 30 2597-2613 (2015) [C1]
|
Nova | |||||||||
2015 |
Nixon B, Bromfield EG, Dun MD, Redgrove KA, McLaughlin EA, Aitken RJ, 'The role of the molecular chaperone heat shock protein A2 (HSPA2) in regulating human sperm-egg recognition', ASIAN JOURNAL OF ANDROLOGY, 17 568-573 (2015) [C1]
|
Nova | |||||||||
2014 |
Bromfield EG, Aitken RJ, Gibb Z, Lambourne SR, Nixon B, 'Capacitation in the presence of methyl-beta-cyclodextrin results in enhanced zona pellucida-binding ability of stallion spermatozoa', REPRODUCTION, 147 153-166 (2014) [C1]
|
Nova | |||||||||
2013 |
Bromfield EG, Nixon B, 'The function of chaperone proteins in the assemblage of protein complexes involved in gamete adhesion and fusion processes', Reproduction, 145 R31-R42 (2013) [C1]
|
Nova | |||||||||
2012 |
Dun MD, Anderson AL, Bromfield EG, Asquith KL, Emmett BJ, McLaughlin EA, et al., 'Investigation of the expression and functional significance of the novel mouse sperm protein, a disintegrin and metalloprotease with thrombospondin type 1 motifs number 10 (ADAMTS10)', International Journal of Andrology, 35 572-589 (2012) [C1]
|
Nova | |||||||||
Show 56 more journal articles |
Conference (2 outputs)
Year | Citation | Altmetrics | Link | ||
---|---|---|---|---|---|
2023 |
Peters AE, McLaughlin EA, Roman SD, Pringle KG, Bromfield EG, Nixon B, Sutherland JM, 'Lysosomal Inhibition of Mouse Oocytes Mimics Key Characteristics of Age-Related Oocyte Quality Decline', REPRODUCTIVE SCIENCES, AUSTRALIA, Brisbane (2023)
|
||||
2022 |
Zhang M, Bromfield EG, Gadella BM, 'Condensation/decondensation of CRISP2 containing intracellular structures in the sperm cell prior to and after fertilization', ANIMAL REPRODUCTION SCIENCE, Bologna, ITALY (2022)
|
Grants and Funding
Summary
Number of grants | 29 |
---|---|
Total funding | $2,153,990 |
Click on a grant title below to expand the full details for that specific grant.
Highlighted grants and funding
Fortifying animal and plant germ cells against proteotoxic stress$263,098
Funding body: ARC (Australian Research Council)
Funding body | ARC (Australian Research Council) |
---|---|
Project Team | Doctor Elizabeth Bromfield, Ms Shannon Smyth |
Scheme | Discovery Early Career Researcher Award (DECRA) |
Role | Lead |
Funding Start | 2021 |
Funding Finish | 2023 |
GNo | G1901392 |
Type Of Funding | C1200 - Aust Competitive - ARC |
Category | 1200 |
UON | Y |
Targeted disruption of lipoxygenase enzymes to prevent oxidative stress-mediated pathologies in the male germline$588,988
Funding body: NHMRC (National Health & Medical Research Council)
Funding body | NHMRC (National Health & Medical Research Council) |
---|---|
Project Team | Professor Brett Nixon, Doctor Elizabeth Bromfield, Distinguished Emeritus Professor John Aitken |
Scheme | Project Grant |
Role | Investigator |
Funding Start | 2019 |
Funding Finish | 2021 |
GNo | G1800389 |
Type Of Funding | C1100 - Aust Competitive - NHMRC |
Category | 1100 |
UON | Y |
Targeted disruption of lipoxygenase enzymes to prevent oxidative stress-mediated pathologies in the male germline$423,938
Funding body: NHMRC (National Health & Medical Research Council)
Funding body | NHMRC (National Health & Medical Research Council) |
---|---|
Project Team | Doctor Elizabeth Bromfield |
Scheme | Early Career Fellowships |
Role | Lead |
Funding Start | 2018 |
Funding Finish | 2021 |
GNo | G1700101 |
Type Of Funding | C1100 - Aust Competitive - NHMRC |
Category | 1100 |
UON | Y |
20213 grants / $583,025
Research Foundation Flanders (FWO) Senior Postdoctoral Fellowship$295,778
Funding body: FWO Review College - Flanders Research Foundation
Funding body | FWO Review College - Flanders Research Foundation |
---|---|
Scheme | Senior Postdoctoral Fellowship |
Role | Lead |
Funding Start | 2021 |
Funding Finish | 2024 |
GNo | |
Type Of Funding | International - Competitive |
Category | 3IFA |
UON | N |
Fortifying animal and plant germ cells against proteotoxic stress$263,098
Funding body: ARC (Australian Research Council)
Funding body | ARC (Australian Research Council) |
---|---|
Project Team | Doctor Elizabeth Bromfield, Ms Shannon Smyth |
Scheme | Discovery Early Career Researcher Award (DECRA) |
Role | Lead |
Funding Start | 2021 |
Funding Finish | 2023 |
GNo | G1901392 |
Type Of Funding | C1200 - Aust Competitive - ARC |
Category | 1200 |
UON | Y |
College of Engineering, Science & Environment Equipment & Infrastructure Investment Scheme$24,149
Funding body: College of Engineering, Science and Environment, University of Newcastle
Funding body | College of Engineering, Science and Environment, University of Newcastle |
---|---|
Project Team | Dr Elizabeth Bromfield, Dr David Skerrett-Byrne, Dr Tessa Lord, Professor Brett Nixon |
Scheme | Equipment and Infrastructure Investment Scheme |
Role | Lead |
Funding Start | 2021 |
Funding Finish | 2022 |
GNo | |
Type Of Funding | Internal |
Category | INTE |
UON | N |
20201 grants / $2,000
Faculty Research Output Accelerator Scheme$2,000
Funding body: College of Engineering, Science and Environment, University of Newcastle
Funding body | College of Engineering, Science and Environment, University of Newcastle |
---|---|
Project Team | Dr. Elizabeth Bromfield, Professor Brett Nixon, Mr Nathan Burke |
Scheme | Lockdown support scheme |
Role | Lead |
Funding Start | 2020 |
Funding Finish | 2020 |
GNo | |
Type Of Funding | Internal |
Category | INTE |
UON | N |
20198 grants / $697,982
Targeted disruption of lipoxygenase enzymes to prevent oxidative stress-mediated pathologies in the male germline$588,988
Funding body: NHMRC (National Health & Medical Research Council)
Funding body | NHMRC (National Health & Medical Research Council) |
---|---|
Project Team | Professor Brett Nixon, Doctor Elizabeth Bromfield, Distinguished Emeritus Professor John Aitken |
Scheme | Project Grant |
Role | Investigator |
Funding Start | 2019 |
Funding Finish | 2021 |
GNo | G1800389 |
Type Of Funding | C1100 - Aust Competitive - NHMRC |
Category | 1100 |
UON | Y |
Bill and Melinda Gates Foundation Grant to run the Gordon Research Conference on Fertilization and Activation of Development$30,157
Funding body: Bill and Melinda Gates Foundation
Funding body | Bill and Melinda Gates Foundation |
---|---|
Project Team | Professor Steve L'Hernault, Dr. Celia Santi, Dr. Elizabeth Bromfield, Dr. Giulia Vigone |
Scheme | Bill and Melinda Gates Foundation Grant |
Role | Investigator |
Funding Start | 2019 |
Funding Finish | 2019 |
GNo | |
Type Of Funding | C3212 - International Not for profit |
Category | 3212 |
UON | N |
Investigating the regulation of androgen production to support healthy male ageing across the life course$19,858
Funding body: Hunter Medical Research Institute
Funding body | Hunter Medical Research Institute |
---|---|
Project Team | Doctor Diane Rebourcet, Doctor Elizabeth Bromfield, Dr Curley Michael |
Scheme | Research Grant |
Role | Investigator |
Funding Start | 2019 |
Funding Finish | 2022 |
GNo | G1901563 |
Type Of Funding | C3300 – Aust Philanthropy |
Category | 3300 |
UON | Y |
National Institute of Child Health and Human Development Grant for GRC on Fertilization and Activation of Development $14,568
Funding body: National Institutes of Health
Funding body | National Institutes of Health |
---|---|
Project Team | Professor Steve L'Hernault, Dr. Celia Santi, Dr. Elizabeth Bromfield, Dr. Giulia Vigone |
Scheme | National Institute of Child Health and Human Development |
Role | Investigator |
Funding Start | 2019 |
Funding Finish | 2019 |
GNo | |
Type Of Funding | C3232 - International Govt - Other |
Category | 3232 |
UON | N |
Research Consultancy$13,554
Funding body: Kazia Laboratories Pty Limited
Funding body | Kazia Laboratories Pty Limited |
---|---|
Project Team | Doctor Elizabeth Bromfield, Professor Brett Nixon |
Scheme | Research Consultancy |
Role | Lead |
Funding Start | 2019 |
Funding Finish | 2019 |
GNo | G1900374 |
Type Of Funding | C3100 – Aust For Profit |
Category | 3100 |
UON | Y |
Ignite EMCR Equipment Grant$13,200
Funding body: Hunter Medical Research Institute (HMRI)
Funding body | Hunter Medical Research Institute (HMRI) |
---|---|
Project Team | Jacinta Martin, Kirsty Pringle, Heather Lee, Elizabeth Bromfield |
Scheme | Early and Mid-Career Equipment Grant |
Role | Investigator |
Funding Start | 2019 |
Funding Finish | 2019 |
GNo | |
Type Of Funding | Internal |
Category | INTE |
UON | N |
National Institute for Environmental Health and Safety grant for Gordon Research Conference on Fertilization and Activation of Development$12,675
Funding body: National Institutes of Health
Funding body | National Institutes of Health |
---|---|
Project Team | Professor Steve L'Hernault, Dr. Celia Santi, Dr. Elizabeth Bromfield, Dr. Giulia Vigone |
Scheme | National Institute of environmental Health and Safety |
Role | Investigator |
Funding Start | 2019 |
Funding Finish | 2019 |
GNo | |
Type Of Funding | C3232 - International Govt - Other |
Category | 3232 |
UON | N |
COUNTESS II IF$4,982
Funding body: Hunter Medical Research Institute
Funding body | Hunter Medical Research Institute |
---|---|
Project Team | Doctor Jessie Sutherland, Doctor Elizabeth Bromfield |
Scheme | Early and Mid-Career Equipment Grant |
Role | Investigator |
Funding Start | 2019 |
Funding Finish | 2019 |
GNo | G1900113 |
Type Of Funding | C3300 – Aust Philanthropy |
Category | 3300 |
UON | Y |
20188 grants / $639,279
Targeted disruption of lipoxygenase enzymes to prevent oxidative stress-mediated pathologies in the male germline$423,938
Funding body: NHMRC (National Health & Medical Research Council)
Funding body | NHMRC (National Health & Medical Research Council) |
---|---|
Project Team | Doctor Elizabeth Bromfield |
Scheme | Early Career Fellowships |
Role | Lead |
Funding Start | 2018 |
Funding Finish | 2021 |
GNo | G1700101 |
Type Of Funding | C1100 - Aust Competitive - NHMRC |
Category | 1100 |
UON | Y |
Fellowship Support PhD Stipend$92,050
Funding body: The University of Newcastle
Funding body | The University of Newcastle |
---|---|
Project Team | Elizabeth G Bromfield |
Scheme | Fellowship support package |
Role | Lead |
Funding Start | 2018 |
Funding Finish | 2021 |
GNo | |
Type Of Funding | Internal |
Category | INTE |
UON | N |
Capillary Flow Two Dimensional High Pressure Liquid Chromatography (HPLC) system$75,761
Funding body: NHMRC (National Health & Medical Research Council)
Funding body | NHMRC (National Health & Medical Research Council) |
---|---|
Project Team | Professor Brett Nixon, Professor Matt Dun, Associate Professor Nikki Verrills, Professor Hubert Hondermarck, Associate Professor Mark Baker, Doctor Elizabeth Bromfield |
Scheme | Equipment Grant |
Role | Investigator |
Funding Start | 2018 |
Funding Finish | 2018 |
GNo | G1800470 |
Type Of Funding | Scheme excluded from IGS |
Category | EXCL |
UON | Y |
School of Environmental and Life Sciences fellowship support$20,000
Funding body: Faculty of Science and Information Technology The University of Newcastle
Funding body | Faculty of Science and Information Technology The University of Newcastle |
---|---|
Project Team | Elizabeth G Bromfield |
Scheme | Fellowship support |
Role | Lead |
Funding Start | 2018 |
Funding Finish | 2021 |
GNo | |
Type Of Funding | Internal |
Category | INTE |
UON | N |
Faculty of Science New Staff Grant$15,000
Funding body: UoN Faculty of Science
Funding body | UoN Faculty of Science |
---|---|
Project Team | Elizabeth G Bromfield |
Scheme | New Staff Grant |
Role | Lead |
Funding Start | 2018 |
Funding Finish | 2018 |
GNo | |
Type Of Funding | Internal |
Category | INTE |
UON | N |
PRC for Chemical Biology and Pharmacology support grant$10,000
Funding body: PRC for Chemical Biology and Pharmacology, The University of Newcastle
Funding body | PRC for Chemical Biology and Pharmacology, The University of Newcastle |
---|---|
Project Team | Elizabeth G Bromfield, Shaun D Roman |
Scheme | PRC for Chemical Biology project support grants 2018 |
Role | Investigator |
Funding Start | 2018 |
Funding Finish | 2018 |
GNo | |
Type Of Funding | Internal |
Category | INTE |
UON | N |
Faculty of Science PVC Conference Assistance Grant$1,500
Funding body: Faculty of Science and Information Technology The University of Newcastle
Funding body | Faculty of Science and Information Technology The University of Newcastle |
---|---|
Project Team | Elizabeth G Bromfield |
Scheme | Faculty PVC Conference Assistance |
Role | Lead |
Funding Start | 2018 |
Funding Finish | 2018 |
GNo | |
Type Of Funding | Internal |
Category | INTE |
UON | N |
Antioxidants journal travel grant$1,030
Funding body: Antioxidants journal
Funding body | Antioxidants journal |
---|---|
Project Team | Elizabeth G Bromfield |
Scheme | 2018 travel grant round |
Role | Lead |
Funding Start | 2018 |
Funding Finish | 2018 |
GNo | |
Type Of Funding | C3212 - International Not for profit |
Category | 3212 |
UON | N |
20177 grants / $180,770
ECR-HDR Candidate Scholarship$92,008
Funding body: The University of Newcastle, Australia
Funding body | The University of Newcastle, Australia |
---|---|
Project Team | Elizabeth G Bromfield |
Scheme | Early Career Researcher HDR Scholarship Scheme: Research Advantage |
Role | Lead |
Funding Start | 2017 |
Funding Finish | 2020 |
GNo | |
Type Of Funding | Internal |
Category | INTE |
UON | N |
UoN Researcher Equipment Grant$49,966
Funding body: The University of Newcastle
Funding body | The University of Newcastle |
---|---|
Project Team | Dr. Zamira Gibb, Professor Brett Nixon, Dr. Shaun Roman, Dr. Jessie Sutherland, Dr. Lisa Mitchell, Dr. Aleona Swegen, Dr. Elizabeth Bromfield, Dr. Kate Redgrove, |
Scheme | Research Advantage Early Career Researcher Equipment Grant Funding |
Role | Investigator |
Funding Start | 2017 |
Funding Finish | 2017 |
GNo | |
Type Of Funding | Internal |
Category | INTE |
UON | N |
Preventing oxidative stress mediated infertility through the targeted disruption of lipoxygenase enzymes$20,000
Funding body: Hunter Medical Research Institute
Funding body | Hunter Medical Research Institute |
---|---|
Project Team | Doctor Elizabeth Bromfield |
Scheme | Project Grant |
Role | Lead |
Funding Start | 2017 |
Funding Finish | 2017 |
GNo | G1701542 |
Type Of Funding | C3300 – Aust Philanthropy |
Category | 3300 |
UON | Y |
Jennie Thomas Medical Research Travel Grant$8,900
Funding body: Hunter Medical Research Institute
Funding body | Hunter Medical Research Institute |
---|---|
Project Team | Miss Jacinta Martin, Professor Brett Nixon, Distinguished Emeritus Professor John Aitken, Doctor Elizabeth Bromfield |
Scheme | Jennie Thomas Medical Research Travel Grant |
Role | Investigator |
Funding Start | 2017 |
Funding Finish | 2017 |
GNo | G1701521 |
Type Of Funding | C3300 – Aust Philanthropy |
Category | 3300 |
UON | Y |
Fertilisation Induced Changes to Oocyte Protective Machinery: Investigating Chemotherapeutics $5,000
Funding body: Hunter Medical Research Institute
Funding body | Hunter Medical Research Institute |
---|---|
Project Team | Professor Brett Nixon, Distinguished Emeritus Professor John Aitken, Doctor Elizabeth Bromfield, Miss Jacinta Martin |
Scheme | Greaves Family Postgraduate Top Up Scholarship in Medical Research |
Role | Investigator |
Funding Start | 2017 |
Funding Finish | 2017 |
GNo | G1700388 |
Type Of Funding | C3300 – Aust Philanthropy |
Category | 3300 |
UON | Y |
Helmsley Charitable Trust Training Grant$2,996
Funding body: Helmsley Charitable Trust
Funding body | Helmsley Charitable Trust |
---|---|
Project Team | Elizabeth G Bromfield |
Scheme | Helmsley Charitable Trust grant to support cross-disciplinary training at Cold Spring Harbour Labs, NY, USA |
Role | Lead |
Funding Start | 2017 |
Funding Finish | 2019 |
GNo | |
Type Of Funding | External |
Category | EXTE |
UON | N |
PVC Conference Assistance Grant$1,900
Funding body: Faculty of Science | University of Newcastle
Funding body | Faculty of Science | University of Newcastle |
---|---|
Project Team | Elizabeth G Bromfield |
Scheme | Faculty PVC Conference Assistance |
Role | Lead |
Funding Start | 2017 |
Funding Finish | 2017 |
GNo | |
Type Of Funding | Internal |
Category | INTE |
UON | N |
20162 grants / $50,934
Beckman Coulter Optima Max-TL, Benchtop Ultra Centrifuge, TLA-110 Fixed-Angle Rotor Package, TLS-55 Swinging Bucket Rotor Package$40,934
Funding body: NHMRC (National Health & Medical Research Council)
Funding body | NHMRC (National Health & Medical Research Council) |
---|---|
Project Team | Professor Brett Nixon, Distinguished Emeritus Professor John Aitken, Professor Eileen McLaughlin, Associate Professor Mark Baker, Doctor Jessie Sutherland, Doctor Elizabeth Bromfield |
Scheme | Equipment Grant |
Role | Investigator |
Funding Start | 2016 |
Funding Finish | 2016 |
GNo | G1601314 |
Type Of Funding | C2200 - Aust Commonwealth – Other |
Category | 2200 |
UON | Y |
Jennie Thomas Medical Research Travel Grant$10,000
Funding body: Hunter Medical Research Institute
Funding body | Hunter Medical Research Institute |
---|---|
Project Team | Doctor Elizabeth Bromfield |
Scheme | Jennie Thomas Medical Research Travel Grant |
Role | Lead |
Funding Start | 2016 |
Funding Finish | 2016 |
GNo | G1600770 |
Type Of Funding | Grant - Aust Non Government |
Category | 3AFG |
UON | Y |
Research Supervision
Number of supervisions
Current Supervision
Commenced | Level of Study | Research Title | Program | Supervisor Type |
---|---|---|---|---|
2024 | PhD | Investigating The Role of SIRT1 in Spermatogonial Stem Cell Function During Developmental and Regenerative Conditions | PhD (Biological Sciences), College of Engineering, Science and Environment, The University of Newcastle | Co-Supervisor |
2020 | PhD | Exploring ALOX15 as a Molecular Conduit between Infertility and Systemic Ill Health in Men | PhD (Biological Sciences), College of Engineering, Science and Environment, The University of Newcastle | Principal Supervisor |
2020 | PhD | Novel Roles for Seminal Fluid Extracellular Vesicles | PhD (Biological Sciences), College of Engineering, Science and Environment, The University of Newcastle | Co-Supervisor |
2019 | PhD | Exploring Protein Aggregation in the Male Germ Line in Response to Oxidative Stress | PhD (Biological Sciences), College of Engineering, Science and Environment, The University of Newcastle | Principal Supervisor |
2019 | PhD | The Role of Autophagy in Oocyte Development and Ageing | PhD (Medical Biochemistry), College of Health, Medicine and Wellbeing, The University of Newcastle | Co-Supervisor |
Past Supervision
Year | Level of Study | Research Title | Program | Supervisor Type |
---|---|---|---|---|
2021 | PhD | Regulation of Sperm Function and Oxidative Stress by Lipoxygenase Enzymes | PhD (Biological Sciences), College of Engineering, Science and Environment, The University of Newcastle | Principal Supervisor |
2019 | PhD | Quality Control Mechanisms Responsible for the Maintenance of Genomic Integrity in the Female Germline | PhD (Biological Sciences), College of Engineering, Science and Environment, The University of Newcastle | Co-Supervisor |
Research Collaborations
The map is a representation of a researchers co-authorship with collaborators across the globe. The map displays the number of publications against a country, where there is at least one co-author based in that country. Data is sourced from the University of Newcastle research publication management system (NURO) and may not fully represent the authors complete body of work.
Country | Count of Publications | |
---|---|---|
Australia | 62 | |
Netherlands | 26 | |
New Zealand | 10 | |
Brazil | 5 | |
United Kingdom | 4 | |
More... |
News
News • 23 Sep 2022
Researchers make new connections between sperm and overall health
Researchers from the Infertility and Reproduction Program at Hunter Medical Research Institute have unearthed a connection between lipid peroxidation that occurs in both poor sperm health and in other diseases.
News • 23 Nov 2020
Four Newcastle researchers selected for funding boost
University of Newcastle researchers have received more than $1.6m in grants from the Australian Research Council’s Discovery Early Career Researcher Award (DECRA) scheme.
News • 11 Oct 2017
UON researchers shine in 2017 NHMRC funding
University of Newcastle researchers have secured more than $6 million in the latest round of National Health and Medical Research Council (NHMRC) funding, including almost $2.5 million for a world-first research centre to test the effectiveness and safety around medicinal applications of a range of cannabinoids.
News • 1 Sep 2016
UON researchers scoop reproductive biology awards
UON reproductive science researchers have been highly recognised with a host of awards at the Society For Reproductive Biology (SRB) Conference.
Dr Elizabeth Bromfield
Position
Honorary Senior Lecturer
PRC Reproductive Science
School of Environmental and Life Sciences
College of Engineering, Science and Environment
Contact Details
elizabeth.bromfield@newcastle.edu.au | |
Phone | (02) 492 16267 |
Link | Research Networks |
Office
Building | Life Science Building |
---|---|
Location | Callaghan University Drive Callaghan, NSW 2308 Australia |