Dr Ryan Duchatel

Dr Ryan Duchatel

Research Assistant

School of Biomedical Sciences and Pharmacy (Medical Biochemistry)

Career Summary

Biography

Ryan is a passionate scientist with an interest in molecular mechanisms of cancer, in particular blood and brain cancers such as Acute Myeloid Leukaemia and paediatric glioma. Ryan currently works as a Research Assistant in the Molecular Oncology laboratory of Dr Matt Dun working on both in vitro and in vivo models of Leukaemia and Diffuse Intrinsic Pontine Glioma.

Ryan studied Biomedical Science graduating with First Class Honours in 2013 and is nearing completion of a PhD in Experimental Pharmacology from the University of Newcastle, Australia. Ryan has been the recipient of prestigious PhD Scholarships including the Ian Scott PhD Scholarship in Mental Health from Australian Rotary Health and the A.M Wood PhD Scholarship from the Schizophrenia Research Institute. 

Ryans PhD has focused on the neurobiology and genetics underpinning schizophrenia conducting studies examining how infections during pregnancy contribute to the development of schizophrenia in subsequent offspring. In particular, Ryan is investigating why people with schizophrenia have more inhibitory interneurons in the white matter underneath the cortex of brain and how maternal immune activation relates to this. Throughout his PhD, Ryan has developed extensive skills in cellular and molecular biology, including utilising both in vitro and in vivo models. 

Qualifications

  • Doctor of Philosophy, University of Newcastle
  • Bachelor of Biomedical Sciences, University of Newcastle
  • Bachelor of Biomedical Sciences (Hons), University of Newcastle

Keywords

  • Brain Cancer
  • Cancer Biology
  • Molecular Oncology
  • Neurobiology
  • Neuroscience
  • Schizophrenia

Fields of Research

Code Description Percentage
110106 Medical Biochemistry: Proteins and Peptides (incl. Medical Proteomics) 20
111201 Cancer Cell Biology 80
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Publications

For publications that are currently unpublished or in-press, details are shown in italics.


Journal article (3 outputs)

Year Citation Altmetrics Link
2018 Duchatel RJ, Meehan CL, Harms LR, Michie PT, Bigland MJ, Smith DW, et al., 'Increased complement component 4 (C4) gene expression in the cingulate cortex of rats exposed to late gestation immune activation', SCHIZOPHRENIA RESEARCH, 199 442-444 (2018)
DOI 10.1016/j.schres.2018.03.035
Co-authors Paul Tooney, Phillip Jobling, Douglas Smith, Pat Michie, Deborah Hodgson, Lauren Harms
2018 Duchatel RJ, Meehan CL, Harms LR, Michie PT, Bigland MJ, Smith DW, et al., 'Late gestation immune activation increases IBA1-positive immunoreactivity levels in the corpus callosum of adult rat offspring', Psychiatry Research, 266 175-185 (2018) [C1]

© 2018 Animal models of maternal immune activation study the effects of infection, an environmental risk factor for schizophrenia, on brain development. Microglia activation and c... [more]

© 2018 Animal models of maternal immune activation study the effects of infection, an environmental risk factor for schizophrenia, on brain development. Microglia activation and cytokine upregulation may have key roles in schizophrenia neuropathology. We hypothesised that maternal immune activation induces changes in microglia and cytokines in the brains of the adult offspring. Maternal immune activation was induced by injecting polyriboinosinic:polyribocytidylic acid into pregnant rats on gestational day (GD) 10 or GD19, with brain tissue collected from the offspring at adulthood. We observed no change in Iba1, Gfap, IL1-ß and TNF-a mRNA levels in the cingulate cortex (CC) in adult offspring exposed to maternal immune activation. Prenatal exposure to immune activation had a significant main effect on microglial IBA1-positive immunoreactive material (IBA1+IRM) in the corpus callosum; post-hoc analyses identified a significant increase in GD19 offspring, but not GD10. No change in was observed in the CC. In contrast, maternal immune activation had a significant main effect on GFAP+IRM in the CC at GD19 (not GD10); post-hoc analyses only identified a strong trend towards increased GFAP+IRM in the GD19 offspring, with no white matter changes. This suggests late gestation maternal immune activation causes subtle alterations to microglia and astrocytes in the adult offspring.

DOI 10.1016/j.psychres.2018.05.063
Co-authors Rohan Walker, Paul Tooney, Pat Michie, Phillip Jobling, Deborah Hodgson, Douglas Smith, Lauren Harms
2016 Duchatel RJ, Jobling P, Graham BA, Harms LR, Michie PT, Hodgson DM, Tooney PA, 'Increased white matter neuron density in a rat model of maternal immune activation - Implications for schizophrenia', Progress in Neuro-Psychopharmacology and Biological Psychiatry, 65 118-126 (2016) [C1]

© 2015. Interstitial neurons are located among white matter tracts of the human and rodent brain. Post-mortem studies have identified increased interstitial white matter neuron (I... [more]

© 2015. Interstitial neurons are located among white matter tracts of the human and rodent brain. Post-mortem studies have identified increased interstitial white matter neuron (IWMN) density in the fibre tracts below the cortex in people with schizophrenia. The current study assesses IWMN pathology in a model of maternal immune activation (MIA); a risk factor for schizophrenia. Experimental MIA was produced by an injection of polyinosinic:polycytidylic acid (PolyI:C) into pregnant rats on gestational day (GD) 10 or GD19. A separate control group received saline injections. The density of neuronal nuclear antigen (NeuN<sup>+</sup>) and somatostatin (SST<sup>+</sup>) IWMNs was determined in the white matter of the corpus callosum in two rostrocaudally adjacent areas in the 12week old offspring of GD10 (n=10) or GD19 polyI:C dams (n=18) compared to controls (n=20). NeuN<sup>+</sup> IWMN density trended to be higher in offspring from dams exposed to polyI:C at GD19, but not GD10. A subpopulation of these NeuN<sup>+</sup> IWMNs was shown to express SST. PolyI:C treatment of dams induced a significant increase in the density of SST<sup>+</sup> IWMNs in the offspring when delivered at both gestational stages with more regionally widespread effects observed at GD19. A positive correlation was observed between NeuN<sup>+</sup> and SST<sup>+</sup> IWMN density in animals exposed to polyI:C at GD19, but not controls. This is the first study to show that MIA increases IWMN density in adult offspring in a similar manner to that seen in the brain in schizophrenia. This suggests the MIA model will be useful in future studies aimed at probing the relationship between IWMNs and schizophrenia.

DOI 10.1016/j.pnpbp.2015.09.006
Citations Scopus - 5Web of Science - 4
Co-authors Brett Graham, Lauren Harms, Paul Tooney, Deborah Hodgson, Phillip Jobling, Pat Michie

Conference (3 outputs)

Year Citation Altmetrics Link
2018 Duchatel R, Jackson E, Verrills N, Cain J, Monje M, Alvaro F, Dun M, 'Investigating ACVR1 and PI3K as Novel Therapeutic Targets in H3.1 K27M+Diffuse Intrinsic Pontine Glioma', ASIA-PACIFIC JOURNAL OF CLINICAL ONCOLOGY (2018)
Co-authors Nikki Verrills, Matt Dun
2018 Germon Z, Sillar J, Murray H, Duchatel R, Al-mazi J, Verrills N, Dun M, 'Intracellular Oxidative Stress Modulates FLT3 Regulatory Proteins Contributing to Oncogenic Signaling in Acute Myeloid Leukemia', ASIA-PACIFIC JOURNAL OF CLINICAL ONCOLOGY (2018)
Co-authors Nikki Verrills, Matt Dun
2015 Duchatel R, Jobling P, Graham B, Harms L, Michie P, Hodgson D, Tooney P, 'Modelling white matter neuron pathology in schizophrenia using maternal immune activation', JOURNAL OF NEUROCHEMISTRY, Cairns, AUSTRALIA (2015) [E3]
Co-authors Brett Graham, Paul Tooney, Pat Michie, Lauren Harms, Phillip Jobling, Deborah Hodgson
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Grants and Funding

Summary

Number of grants 5
Total funding $166,000

Click on a grant title below to expand the full details for that specific grant.


20185 grants / $166,000

Proteomic architecture of diffuse pontine intrinsic glioma$100,000

Funding body: McDonald Jones Charitable Foundation

Funding body McDonald Jones Charitable Foundation
Project Team Doctor Matt Dun, Doctor Frank Alvaro, Doctor Ryan Duchatel, Ms Heather Murray, Associate Professor David Ziegler
Scheme Postdoctoral fellowship
Role Investigator
Funding Start 2018
Funding Finish 2020
GNo G1801130
Type Of Funding C3120 - Aust Philanthropy
Category 3120
UON Y

Enhancing the efficacy of new inhibitors targeting the PI3K–AKT–mTOR signalling axis for the treatment of high-grade diffuse intrinsic pontine gliomas (DIPG)$30,000

Funding body: Hunter Medical Research Institute

Funding body Hunter Medical Research Institute
Project Team Doctor Matt Dun, Doctor Ryan Duchatel, Doctor Adjanie Patabendige
Scheme Project Grant
Role Investigator
Funding Start 2018
Funding Finish 2018
GNo G1801386
Type Of Funding C3120 - Aust Philanthropy
Category 3120
UON Y

Detection & monitoring of disease progression in high grade brain cancer$19,000

Funding body: Hunter Medical Research Institute

Funding body Hunter Medical Research Institute
Project Team Doctor Matt Dun, Doctor Muhammad Jamaluddin, Doctor Ryan Duchatel, Doctor Frank Alvaro
Scheme Project Grant
Role Investigator
Funding Start 2018
Funding Finish 2018
GNo G1801235
Type Of Funding C3120 - Aust Philanthropy
Category 3120
UON Y

Building international collaborations for DIPG research$10,000

Funding body: Hunter Medical Research Institute

Funding body Hunter Medical Research Institute
Project Team Doctor Ryan Duchatel, Doctor Matt Dun
Scheme Jennie Thomas Medical Research Travel Grant
Role Lead
Funding Start 2018
Funding Finish 2018
GNo G1801371
Type Of Funding C3120 - Aust Philanthropy
Category 3120
UON Y

Targeting oncogenic signalling in DIPG using drugs that cross the blood brain barrier.$7,000

Funding body: Australian Communities Foundation

Funding body Australian Communities Foundation
Project Team Doctor Matt Dun, Doctor Ryan Duchatel, Ms Terina Vale
Scheme Isabella and Marcus Paediatric Brainstem Tumour Fund
Role Investigator
Funding Start 2018
Funding Finish 2018
GNo G1800977
Type Of Funding C3112 - Aust Not for profit
Category 3112
UON Y
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Dr Ryan Duchatel

Position

Research Assistant
Cancer Signalling
School of Biomedical Sciences and Pharmacy
Faculty of Health and Medicine

Focus area

Medical Biochemistry

Contact Details

Email ryan.duchatel@newcastle.edu.au
Phone (02) 49854489
Mobile (+61) 419268714

Office

Room LS339
Building Life Science
Location Callaghan
University Drive
Callaghan, NSW 2308
Australia
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