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Dr Ian Grainge

ARC Future Fellow

School of Environmental and Life Sciences (Biological Sciences)

From Oxford to Newcastle

Dr Ian Grainge

The journey to biology research began for Dr Ian Grainge almost 20 years ago in the United Kingdom.

Two years into an undergraduate degree, Grainge's initial interest in Chemistry had begun to dwindle.  He switched to genetics before obtaining his PhD in biochemistry and he hasn't looked back since.

From there he took his interest in research around the world. This included  working on a natural yeast plasmid in Texas for three years before returning to work for Cancer Research UK, looking at replication proteins and protein structures (x-ray crystallography).

After doing more work on bacteria in Oxford, Grainge's idea was to link together biochemistry (how proteins work in test tubes) to the actual structural information and has carried on with this theme in his current research.

"At the moment we're trying to understand the molecular details of one of the motor proteins and the way it can bind and convert chemical energy into actual movement of DNA" explained Grainge.

"This FtsK protein is a very efficient molecular pump that moves the DNA through the cell and we're trying to understand the molecular details of how this protein machine works. It is found in most bacteria and plays an important role in the cell, co-ordinating the vital process of cell division and chromosome unlinking, which is one way bacteria keep their chromosome intact" he added.

The four year ARC Future Fellowship that Grainge was awarded in 2012 will contribute to this research, which could potentially form the basis for developing a new antibiotic to fight bacteria.

"It's amazing to get the grant both for opportunity for research and the recognition that somebody thinks my work is important. I've got more than enough work to do over the term of the Fellowship and I'll try to get students interested in the work as well to get them helping me on the project", said Grainge.

Mathematicians will also be involved with the project to try to understand the way in which the protein affects the shape of the DNA, known as DNA Topology, and how that affects the outcomes of these reactions.

Grainge's move to Newcastle 2010 was a big step to becoming independent and setting up his own lab, which was a big motivating factor in his decision making. Since his time at the University of Newcastle, he has obtained four grants, including the Fellowship, which is a major achievement.

While the future appears very busy for Grainge, he hopes to take up a few hobbies while based in Newcastle.

"I'd like to try surfing some day. I've just been too scared to try it so far. Apart from that I'm happy to stay at the University of Newcastle. I like it here," he said.

Dr Ian Grainge

From Oxford to Newcastle

Dr Ian Grainge is interested in all aspects of how bacteria pass on their genetic information, from DNA replication to chromosome segregation and accurate

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Career Summary

Biography

I am interested in all aspects of how bacteria pass on their genetic information, from DNA replication to chromosome segregation and accurate cell division. My current research focuses on two main topics. Firstly what happens when the process of DNA replication runs into a blockage and stops- how can the cell recover to restart the vital process of copying its DNA? A large number of homologous recombination proteins have been implicated in the processing of collapsed DNA replication forks and their roles, and the pathways used, will be investigated in living cells. Secondly, is the study of the FtsK protein, which co-ordinates the processes of cell division, chromosome unlinking and chromosome segregation in bacteria. Each of these processes has to be completed in a timely manner to allow the cell to divide to produce offspring with a full genetic content. The DNA translocase protein, FtsK, is a key protein in each of these processes, and could additionally act as a cell division checkpoint. Post-doctoral work: Department of Biochemistry, University of Oxford (2005-2009) Cancer Research UK (2000-2004) University of Texas at Austin (1997-2000) PhD: University of Oxford, UK (1994-1997)

Research Expertise
DNA replication in bacteria: restart of stalled replication forks The chromosome of E. coli is a circular DNA molecule which is replicated bi-directionally from a single origin (OriC). Multiple copies of the tetracycline operator (tetO) have been placed in the chromosome 16kb to one side of the origin of replication. Expression of a fluorescent tetracycline repressor (TetR-YFP) allows direct visualization of this region in a fluorescence microscope. Using this system, the origin can effectively be followed during duplication and on through the cell cycle. It was found that overexpression of tetR led to cell inviability. The viability of the cells could be recovered by addition of the effector molecule, anhydrous tetracycline (AT) which reduces the binding of TetR to tetO. Analysis of replication in cells overexpressing TetR showed that the array formed an effective block to replication forks. 2-D gel analysis shows that replication forks can proceed fewer than 500 bp into the tetO array before stalling occurs. Further it is seen that addition of AT leads to a very rapid restart of these stalled forks. Restart of the forks was examined in recA and recB mutant strains and it was found that restart occurred with very similar kinetics to those seen in a wild-type background. This has led us to propose that a stalled replication fork is stable in E. coli for a period of at least 2 hours and that restart does not require recombination. Current work is focusing on what happens when the replisome, stalled at the tetO array block, is disassembled, with a view to understanding replication fork restart pathways and kinetics in vivo. Using this system replication restart can be followed in various mutant backgrounds by examination of fluorescent repressor operators, and by using 2-D gels, to effectively dissect in vivo restart pathways. FtsK: a fast molecular motor The multifunctional FtsK protein is involved in cell division and DNA segregation in E. coli. The C-terminal portion of this large protein forms a hexameric ring with the ability to translocate DNA at speeds of ~ 5kb/sec. The FtsK protein is loaded on DNA in a specific orientation by interactions with polarized sequences on the chromosome which ensure that the protein will subsequently move towards the dif site located in the terminus of the chromosome. Once there, FtsK also interacts with the site-specific recombinase XerD to promote recombination between two dif sites. Further, as a result of translocation the two recombining dif sites are brought together in a topologically simple manner so that recombination leads to a simplification of topology, and eventually chromosome unlinking. Using a variety of biochemical techniques the mechanism of directed loading upon DNA, DNA translocation and activation of recombination within a specific synapse topology is being investigated. Using covalently linked multimers of the translocase protion of the protein, hexameric rings can be formed within which mutations can be targeted to specific subunits. This allows more defined analysis of the mechanism of loading and translocation than would otherwise be possible.

Teaching Expertise
Present course taught: BIOL2010- Biochemistry CHEM3550 Medicinal Chemistry BIOL3100 Microbiology BIOL2230 Biomolecules Courses lectured at the University of Oxford, Department of Biochemistry: Modern Molecular Biology: Methods DNA: Replication and Recombination

Administrative Expertise
Member of Institutional Biosafety Committee Member of Faculty Research and Research Training Committee Convenor of Biological Sciences seminar series.


Qualifications

  • PhD, University of Oxford - UK
  • Bachelor of Arts, University of Cambridge - UK
  • Master of Arts, University of Cambridge - UK

Keywords

  • BIOL2010
  • BIOL2230
  • BIOL3100
  • CHEM3550
  • Cell division
  • Chromosome segregation
  • DNA translocation
  • Microbiology
  • Novel antibiotics
  • Site-specific recombination

Fields of Research

Code Description Percentage
030499 Medicinal and Biomolecular Chemistry not elsewhere classified 10
060199 Biochemistry and Cell Biology not elsewhere classified 70
060599 Microbiology not elsewhere classified 20

Professional Experience

UON Appointment

Title Organisation / Department
Lecturer University of Newcastle
School of Environmental and Life Sciences
Australia

Academic appointment

Dates Title Organisation / Department
1/11/2012 -  Fellow ARC

ARC - Discovery - Future Fellowships

University of Newcastle
Australia

Membership

Dates Title Organisation / Department
1/01/2012 -  Membership - Australian Society for Microbiology Australian Society for Microbiology
Australia
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Publications

For publications that are currently unpublished or in-press, details are shown in italics.


Chapter (5 outputs)

Year Citation Altmetrics Link
2012 Doherty GP, Mettrick KA, Grainge IR, Lewis PJ, 'Imaging fluorescent protein fusions in live bacteria', Methods in Microbiology, Academic Press, Kidlington, Ox 107-126 (2012) [B1]
Co-authors Peter Lewis
2007 Grainge I, Sherratt D, 'Site specific recombination', Topics in Current Genetics: Molecular Genetics of Recombination, Springer, Berlin, Germany 1423-1433 (2007) [B1]
2005 Grainge IRF, Jayaram M, 'Introduction to site-specific recombination', The Dynamic Bacterial Genome, Cambridge University Press, Cambridge, United Kingdom 33-82 (2005) [B1]
2005 Voziyanov Y, Grainge I, Jayaram M, 'Applications of fungal site-specific recombination as a tool in biotechnology and basic biology', 189-210 (2005) [B1]

Two classes of conservative site-specific recombinases, those belonging to the tyrosine and serine families, have been identified, and several of its members characterized in gene... [more]

Two classes of conservative site-specific recombinases, those belonging to the tyrosine and serine families, have been identified, and several of its members characterized in genetic and biochemical detail. These families are named after the active site amino acid, tyrosine or serine, that is utilized as the nucleophile during the strand breaking step of recombination. The Flp recombinase encoded by the 2 micron plasmid of Saccharomyces cerevisiae and related recombinases encoded by similar plasmids found in other yeast species belong to the tyrosine family. The Flp protein has provided several insights into the mechanism of target DNA recognition, strand cleavage and strand exchange during the recombination reaction. Here we describe how the Flp system has been used as a tool for tackling basic and applied problems in biology. © 2005 Elsevier B.V. All rights reserved.

DOI 10.1016/S1874-5334(05)80010-3
2002 Jayaram M, Grainge IRF, Tribble GD, 'Site-specific DNA recombination mediated by the Flp protein of Saccharomyces cerevisiae', Mobile DNA II, ASM Press, Washington, DC/USA 192-218 (2002) [B1]
Show 2 more chapters

Journal article (30 outputs)

Year Citation Altmetrics Link
2014 Guo P, Grainge I, Zhao Z, Vieweger M, 'Two classes of nucleic acid translocation motors: Rotation and revolution without rotation', Cell and Bioscience, 4 (2014) [C1]

Biomotors are extensively involved in biological processes including cell mitosis, bacterial binary fission, DNA replication, DNA repair, homologous recombination, Holliday juncti... [more]

Biomotors are extensively involved in biological processes including cell mitosis, bacterial binary fission, DNA replication, DNA repair, homologous recombination, Holliday junction resolution, RNA transcription, and viral genome packaging. Traditionally, they were classified into two categories including linear and rotation motors. In 2013, a third class of motor by revolution mechanism without rotation was discovered. In this issue of " Structure and mechanisms of nanomotors in the cells" , four comprehensive reviews are published to address the latest advancements of the structure and motion mechanism of a variety of biomotors in archaea, animal viruses, bacteria, and bacteriophages.

DOI 10.1186/2045-3701-4-54
Citations Scopus - 2Web of Science - 1
2013 Shimokawa K, Ishihara K, Grainge I, Sherratt DJ, Vazquez M, 'FtsK-dependent XerCD-dif recombination unlinks replication catenanes in a stepwise manner.', Proc Natl Acad Sci U S A, 110 20906-20911 (2013) [C1]
DOI 10.1073/pnas.1308450110
Citations Scopus - 8Web of Science - 8
2013 Grainge I, 'Simple topology: FtsK-directed recombination at the dif site', BIOCHEMICAL SOCIETY TRANSACTIONS, 41 595-600 (2013) [C1]
DOI 10.1042/BST20120299
Citations Scopus - 6Web of Science - 6
2011 Grainge IR, Lesterlin C, Sherratt DJ, 'Activation of XerCD-dif recombination by the FtsK DNA translocase', Nucleic Acids Research, 39 5140-5148 (2011) [C1]
DOI 10.1093/nar/gkr078
Citations Scopus - 27Web of Science - 27
2010 Crozat E, Meglio A, Allemand J-F, Chivers CE, Howarth M, Venien-Bryan C, et al., 'Separating speed and ability to displace roadblocks during DNA translocation by FtsK.', The EMBO Journal, 29 1423-1433 (2010) [C1]
DOI 10.1038/emboj.2010.29
Citations Scopus - 22Web of Science - 22
2010 Sherratt DJ, Arciszewska L, Crozat E, Graham J, Grainge IRF, 'The Escherichia coli DNA translocase FtsK.', Biochemical Society Transactions, 38 395-398 (2010) [C1]
DOI 10.1042/BST0380395
Citations Scopus - 29Web of Science - 29
2010 Grainge IR, 'FtsK - a bacterial cell division checkpoint?', Molecular Microbiology, 78 1055-1057 (2010) [C1]
DOI 10.1111/j.1365-2958.2010.07411.x
Citations Scopus - 5Web of Science - 5
2010 Crozat E, Grainge IR, 'FtsK DNA translocase: The fast motor that knows where it's going', ChemBioChem, 11 2232-2243 (2010) [C1]
DOI 10.1002/cbic.201000347
Citations Scopus - 21Web of Science - 21
2008 Lowe J, Ellonen A, Allen MD, Atkinson C, Sherratt DJ, Grainge IRF, 'Molecular mechanism of sequence-directed DNA loading and translocation by FtsK', Molecular Cell, 31 498-509 (2008) [C1]
DOI 10.1016/j.molcel.2008.05.027
Citations Scopus - 56Web of Science - 57
2008 Atanassova N, Grainge IRF, 'Biochemical Characterization of the Minichromosome Maintenance (MCM) Protein of the Crenarchaeote Aeropyrum pernix and Its Interactions with the Origin Recognition Complex (ORC) Proteins', Biochemistry, 47 1336-1337 (2008) [C1]
DOI 10.1021/bi801479s
Citations Scopus - 9Web of Science - 10
2008 Grainge IRF, 'Sporulation: SpoIIIE is the key to cell differentiation', Current Biology, 18 871-872 (2008) [C1]
DOI 10.1016/j.cub.2008.07.047
Citations Scopus - 4Web of Science - 4
2007 Grainge I, Sherratt DJ, 'Site-specific recombination', Topics in Current Genetics, 17 27-52 (2007)

Site-specific recombination is a reaction in which a pair of genetically defined sites undergoes reciprocal exchange ("crossing-over") via a recombinase-mediated DNA breakage and ... [more]

Site-specific recombination is a reaction in which a pair of genetically defined sites undergoes reciprocal exchange ("crossing-over") via a recombinase-mediated DNA breakage and joining process. Such reactions have a wide range of biological outcomes, from integration and excision of virus genomes into and out of host chromosomes, to acquisition of novel genes and drug resistance, and even facilitating bacterial chromosome segregation. Two distinct families of recombinases exist, designated by their active site residues. In both these families recombination is carried out by a core of four recombinase monomers acting at two synapsed DNA sites. In many cases additional recombinase monomers and/or accessory proteins act at adjacent DNA sites to facilitate synapsis and often play a critical role in determining reaction topology. Here, the mechanism of site-specific recombination reactions is examined for both site-specific recombinase families, as well as for related proteins that mediate variant reactions, such as integrons and the integrases of conjugative transposons. © 2006 Springer-Verlag Berlin Heidelberg.

DOI 10.1007/4735_2006_0202
2007 Grainge I, Bregu M, Vazquez M, Sivanathan V, Ip SCY, Sherratt DJ, 'Unlinking chromosome catenanes in vivo by site-specific recombination', EMBO JOURNAL, 26 4228-4238 (2007) [C1]
DOI 10.1038/sj.emboj.7601849
Citations Scopus - 47Web of Science - 50
2006 Grainge I, Gaudier M, Schuwirth BS, Westcott SL, Sandall J, Atanassova N, Wigley DB, 'Biochemical analysis of a DNA replication origin in the archaeon Aeropyrum pernix', JOURNAL OF MOLECULAR BIOLOGY, 363 355-369 (2006) [C1]
DOI 10.1016/jmb.2006.07.076
Citations Scopus - 28Web of Science - 28
2006 Possoz C, Filipe SR, Grainge I, Sherratt DJ, 'Tracking of controlled Escherichia coli replication fork stalling and restart at repressor-bound DNA in vivo', EMBO JOURNAL, 25 2596-2604 (2006) [C1]
DOI 10.1038/sj.emboj.7601155
Citations Scopus - 60Web of Science - 61
2004 Singleton MR, Morales R, Grainge I, Cook N, Isupov MN, Wigley DB, 'Conformational changes induced by nucleotide binding in Cdc6/ORC from Aeropyrum pernix', JOURNAL OF MOLECULAR BIOLOGY, 343 547-557 (2004) [C1]
DOI 10.1016/j.jmb.2004.08.044
Citations Scopus - 54Web of Science - 54
2003 Grainge I, Scaife S, Wigley DB, 'Biochemical analysis of components of the pre-replication complex of Archaeoglobus fulgidus', NUCLEIC ACIDS RESEARCH, 31 4888-4898 (2003) [C1]
DOI 10.1093/nar/gkg662
Citations Scopus - 49Web of Science - 50
2002 Sau AK, Tribble GD, Grainge I, Frohlich RF, Knudsen BR, Jayaram M, 'Biochemical and kinetic analysis of the RNase active sites of the integrase/tyrosine family site-specific recombinases. (vol 276, pg 46612, 2001)', JOURNAL OF BIOLOGICAL CHEMISTRY, 277 6758-6758 (2002)
2002 Sau AK, DeVue Tribble G, Grainge I, Frøhlich RF, Knudsen BR, Jayaram M, 'Erratum: Biochemical and kinetic analysis of the RNase active sites of the integrase/tyrosine family site-specific recombinases (Journal of Biological Chemistry (2001) 276 (46612-46623))', Journal of Biological Chemistry, 277 6758-6758 (2002)
2002 Grainge I, Pathania S, Vologodskii A, Harshey RM, Jayaram M, 'Symmetric DNA sites are functionally asymmetric within Flp and Cre site-specific DNA recombination synapses', JOURNAL OF MOLECULAR BIOLOGY, 320 515-527 (2002) [C1]
DOI 10.1016/S0022-2836(02)00517-X
Citations Scopus - 16Web of Science - 19
2001 Frohlich RF, Hansen SG, Lisby M, Grainge I, Westergaard O, Jayaram M, Knudsen BR, 'Inhibition of Flp recombinase by the topoisomerase I-targeting drugs, camptothecin and NSC-314622', JOURNAL OF BIOLOGICAL CHEMISTRY, 276 6993-6997 (2001) [C1]
DOI 10.1074/jbc.C000901200
Citations Scopus - 1Web of Science - 1
2001 San AK, Tribble GD, Grainge I, Frohlich RF, Knudsen BR, Jayaram M, 'Biochemical and kinetic analysis of the RNase active sites of the integrase/tyrosine family site-specific DNA recombinases', JOURNAL OF BIOLOGICAL CHEMISTRY, 276 46612-46623 (2001)
Citations Scopus - 2Web of Science - 1
2001 Sau AK, Tribble GD, Grainge IRF, Frohlich RF, Knudsen BR, Jayaram M, 'Biochemical and kinetic analysis of the RNase active sites of the integrase/tyrosine family site-specific recombinases', Journal of Biological Chemistry, 276 4661-4662 (2001) [C1]
2001 Grainge I, Lee J, Xu CJ, Jayaram M, 'DNA recombination and RNA cleavage activities of the Flp protein: Roles of two histidine residues in the orientation and activation of the nucleophile for strand cleavage', JOURNAL OF MOLECULAR BIOLOGY, 314 717-733 (2001) [C1]
DOI 10.1006/jmbi.2001.5194
Citations Scopus - 3Web of Science - 3
2000 Grainge I, Buck D, Jayaram M, 'Geometry of site alignment during int family recombination: Antiparallel synapsis by the Flp recombinase', JOURNAL OF MOLECULAR BIOLOGY, 298 749-764 (2000) [C1]
DOI 10.1006/jmbi.2000.3769
Citations Scopus - 40Web of Science - 45
1999 Grainge I, Sherratt DJ, 'Xer site-specific recombination - DNA strand rejoining by recombinase XerC', JOURNAL OF BIOLOGICAL CHEMISTRY, 274 6763-6769 (1999) [C1]
DOI 10.1074/jbc.274.10.6763
Citations Scopus - 4Web of Science - 5
1999 Lee J, Jayaram M, Grainge I, 'Wild-type Flp recombinase cleaves DNA in trans', EMBO JOURNAL, 18 784-791 (1999) [C1]
DOI 10.1093/emboj/18.3.784
Citations Scopus - 30Web of Science - 28
1998 Xu CJ, Grainge I, Lee J, Harshey RM, Jayaram M, 'Unveiling two distinct ribonuclease activities and a topoisomerase activity in a site-specific DNA recombinase', MOLECULAR CELL, 1 729-739 (1998) [C1]
DOI 10.1016/S1097-2765(00)80072-6
Citations Scopus - 26Web of Science - 26
1997 Arciszewska L, Grainge IRF, Sherratt DJ, 'Action of site-specific recombinases XerC and XerD on tethered Holliday junctions', The EMBO Journal, 16 3731-3743 (1997) [C1]
Citations Scopus - 39Web of Science - 37
1995 Arciszewska L, Grainge IRF, Sherratt DJ, 'EFFECTS OF HOLLIDAY JUNCTION POSITION ON XER-MEDIATED RECOMBINATION IN-VITRO', The EMBO Journal, 14 2651-2660 (1995) [C1]
Citations Scopus - 27Web of Science - 27
Show 27 more journal articles

Review (1 outputs)

Year Citation Altmetrics Link
1999 Grainge I, Jayaram M, 'The integrase family of recombinases: organization and function of the active site', MOLECULAR MICROBIOLOGY (1999) [D1]
DOI 10.1046/j.1365-2958.1999.01493.x
Citations Scopus - 96Web of Science - 95
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Grants and Funding

Summary

Number of grants 13
Total funding $2,321,884

Click on a grant title below to expand the full details for that specific grant.


20141 grants / $10,000

DNA Replication fork stability, collapse and processing in living Escherichia coli cells$10,000

Funding body: University of Newcastle

Funding body University of Newcastle
Project Team Doctor Ian Grainge
Scheme Near Miss Grant
Role Lead
Funding Start 2014
Funding Finish 2014
GNo G1301382
Type Of Funding Internal
Category INTE
UON Y

20134 grants / $76,144

DVC(R) Research Support for Future Fellow (FT12)$60,000

Funding body: University of Newcastle

Funding body University of Newcastle
Project Team Doctor Ian Grainge
Scheme Special Project Grant
Role Lead
Funding Start 2013
Funding Finish 2013
GNo G1201101
Type Of Funding Internal
Category INTE
UON Y

Development of new genetic tools for protein knockouts in pathogenic bacteria$13,129

Funding body: University of Newcastle - Faculty of Science & IT

Funding body University of Newcastle - Faculty of Science & IT
Project Team Doctor Ian Grainge
Scheme Strategic Small Grant
Role Lead
Funding Start 2013
Funding Finish 2013
GNo G1401062
Type Of Funding Internal
Category INTE
UON Y

Faculty PVC Conference Assistance Grant 2013$2,000

Funding body: University of Newcastle - Faculty of Science & IT

Funding body University of Newcastle - Faculty of Science & IT
Project Team Doctor Ian Grainge
Scheme PVC Conference Assistance Grant
Role Lead
Funding Start 2013
Funding Finish 2013
GNo G1401158
Type Of Funding Internal
Category INTE
UON Y

Characterisation of the interaction between the essential bacterial transcription factor NusA and RNA Polymerase$1,015

Funding body: Australian Synchrotron

Funding body Australian Synchrotron
Project Team Doctor Ian Grainge
Scheme Travel Grant
Role Lead
Funding Start 2013
Funding Finish 2013
GNo G1301024
Type Of Funding Other Public Sector - State
Category 2OPS
UON Y

20123 grants / $1,030,757

Characterization of a powerful molecular motor, the FtsK DNA translocase$709,318

Funding body: ARC (Australian Research Council)

Funding body ARC (Australian Research Council)
Project Team Doctor Ian Grainge
Scheme Future Fellowships
Role Lead
Funding Start 2012
Funding Finish 2012
GNo G1101070
Type Of Funding Aust Competitive - Commonwealth
Category 1CS
UON Y

Targeting nucleic acid synthesis and cell division in gram-negative bacterial pathogens$311,439

Funding body: NHMRC (National Health & Medical Research Council)

Funding body NHMRC (National Health & Medical Research Council)
Project Team Professor Nicholas Dixon, Associate Professor Elizabeth Harry, Professor Peter Lewis, Associate Professor Aaron Oakley, Doctor Ian Grainge
Scheme Project Grant
Role Investigator
Funding Start 2012
Funding Finish 2012
GNo G1101133
Type Of Funding Aust Competitive - Commonwealth
Category 1CS
UON Y

Ultrasonic Homogenizer System and -80 ºC Freezers for chemical and biological sample storage$10,000

Funding body: NHMRC (National Health & Medical Research Council)

Funding body NHMRC (National Health & Medical Research Council)
Project Team Professor Adam McCluskey, Professor Eileen McLaughlin, Professor Peter Lewis, Ms Belinda Nixon, Doctor Shaun Roman, Doctor Jennette Sakoff, Doctor Ian Grainge
Scheme Equipment Grant
Role Investigator
Funding Start 2012
Funding Finish 2012
GNo G1100986
Type Of Funding Other Public Sector - Commonwealth
Category 2OPC
UON Y

20114 grants / $1,199,983

Molecular characterization of the role of FtsK in chromosome unlinking and segregation.$455,022

Funding body: NHMRC (National Health & Medical Research Council)

Funding body NHMRC (National Health & Medical Research Council)
Project Team Doctor Ian Grainge
Scheme Project Grant
Role Lead
Funding Start 2011
Funding Finish 2011
GNo G1000271
Type Of Funding Aust Competitive - Commonwealth
Category 1CS
UON Y

Chemical Biology$444,961

Funding body: University of Newcastle

Funding body University of Newcastle
Project Team Professor Adam McCluskey, Doctor Warwick Belcher, Doctor Ian Grainge, Professor Christopher Grof, Professor Peter Lewis, Professor Eileen McLaughlin, Doctor Shaun Roman, Conjoint Professor Ray Rose, Doctor Jennette Sakoff, Doctor Nikki Verrills
Scheme Priority Research Centre
Role Investigator
Funding Start 2011
Funding Finish 2011
GNo G1100052
Type Of Funding Internal
Category INTE
UON Y

DNA Replication fork processing and recovery in living Escherichia coli cells$285,000

Funding body: ARC (Australian Research Council)

Funding body ARC (Australian Research Council)
Project Team Doctor Ian Grainge
Scheme Discovery Projects
Role Lead
Funding Start 2011
Funding Finish 2011
GNo G1000148
Type Of Funding Aust Competitive - Commonwealth
Category 1CS
UON Y

Eppendorf mastercycler pro with thermomixer comfort and 5430R centrifuge$15,000

Funding body: NHMRC (National Health & Medical Research Council)

Funding body NHMRC (National Health & Medical Research Council)
Project Team Professor Eileen McLaughlin, Professor Peter Lewis, Professor Adam McCluskey, Conjoint Professor Keith Jones, Associate Professor Brett Nixon, Doctor Shaun Roman, Doctor Jennette Sakoff, Doctor Ian Grainge, Doctor Janet Holt, Doctor Xiao Yang
Scheme Equipment Grant
Role Investigator
Funding Start 2011
Funding Finish 2011
GNo G1100028
Type Of Funding Other Public Sector - Commonwealth
Category 2OPC
UON Y

20101 grants / $5,000

Chromosome stability in pathogenic bacteria$5,000

Funding body: University of Newcastle

Funding body University of Newcastle
Project Team Doctor Ian Grainge
Scheme New Staff Grant
Role Lead
Funding Start 2010
Funding Finish 2010
GNo G1000625
Type Of Funding Internal
Category INTE
UON Y
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Research Supervision

Number of supervisions

Completed1
Current6

Total current UON EFTSL

PhD3.2

Current Supervision

Commenced Level of Study Research Title / Program / Supervisor Type
2015 PhD The Role of Helicases in Replication Fork Processing and Restart Following Stalling in E.coli
Biological Sciences, Faculty of Science and Information Technology, The University of Newcastle
Principal Supervisor
2015 PhD Elucidating the Function of the Novel Transcription Factor AtfA and its Relevance to Virulence and Persistence in the y Proteobacteria
Biological Sciences, Faculty of Science and Information Technology, The University of Newcastle
Co-Supervisor
2014 PhD Frequency of Chromosome Dimers Resulting From Replication Fork Processing Following Blockage or Collapse
Biological Sciences, Faculty of Science and Information Technology, The University of Newcastle
Principal Supervisor
2013 PhD Understanding Chromosome Dimer Resolution Systems of a Pathogenic Bacteria at a Molecular Level
Biological Sciences, Faculty of Science and Information Technology, The University of Newcastle
Principal Supervisor
2012 PhD Development of Genetic Tools for the Quantitative Assessment of Gene Depletion in Pathogenic Bacteria
Biological Sciences, Faculty of Science and Information Technology, The University of Newcastle
Principal Supervisor
2012 PhD Bacterial Transcription Factors NusB, NusE ATFA and p as Targets for Antibiotic Research
Biological Sciences, Faculty of Science and Information Technology, The University of Newcastle
Co-Supervisor

Past Supervision

Year Level of Study Research Title / Program / Supervisor Type
2014 PhD Increased Understanding of the Molecular Interactions Involved in Bacterial Transcription and Recombination
Biological Sciences, Faculty of Science and Information Technology, The University of Newcastle
Co-Supervisor
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Dr Ian Grainge

Position

ARC Future Fellow
School of Environmental and Life Sciences
Faculty of Science and Information Technology

Focus area

Biological Sciences

Contact Details

Email ian.grainge@newcastle.edu.au
Phone 4921 7238
Fax 4921 5472

Office

Room BG09
Building Biological Sciences
Location Callaghan
University Drive
Callaghan, NSW 2308
Australia
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