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Professor Peter Lewis

Professor

School of Environmental and Life Sciences (Biological Sciences)

Career Summary

Biography

My research career has focused on the gram positive bacterium Bacillus subtilis. During my PhD I purified and characterised the replication terminator protein (RTP) and its DNA binding sites (Lewis et al., J. Bacteriol 171, 3564-3567 (1989); Lewis et al., J. Mol. Biol. 214, 73-84 (1990)). These sites are regions of DNA where oppositely moving replication forks meet and newly replicated chromosomes are resolved prior to segregation. During my post-doctoral research I was responsible for the development of cell biological techniques and vectors for visualising gene expression and protein localisation in live bacterial cells (Lewis et al., PNAS 91, 3849-3853 (1994); Lewis et al., Mol Microbiol 13, 655-662 (1994); Lewis and Errington, Microbiology 142, 733-740 (1996); Lewis and Marston, Gene 227, 101-109 (1999); Feucht and Lewis, Gene 264, 289-297 (2001)).

These techniques have been widely adopted by research groups involved in microbial cell biology, and the plasmid vectors have now been distributed directly to well over 100 laboratories world-wide, although they are now also distributed by the Bacillus Genetic Stock Centre. These techniques were instrumental in determining the establishment of compartment specificity of developmentally regulated s-factors during sporulation in B. subtilis (Lewis et al., PNAS 91, 3849-3853 (1994)). I also showed that prespore specific accumulation of the transcription regulator SpoIIAA was responsible for initiation of compartment-specific gene expression during development, that this accumulation was probably due to prespore-specific activity of the phosphatase SpoIIE, and that a programme of proteolysis was initiated following this activation event (Lewis et al., Genes Cell 1, 881-894 (1996); Lewis et al., J. Bacteriol 180, 3276-3284 (1998)). I was also involved in work that showed that the highly conserved SpoIIIE (FtsK) protein is a DNA translocase that moves DNA through a division septum (Wu et al., Genes Dev 9, 1316-1326 (1995)). This was the first example of such a phenomenon, and was a very significant finding as previously it was assumed that DNA was segregated into daughter cells/different compartments prior to division septum formation. Finally, I have shown that transcription and translation are spatially separated within bacteria (Lewis et al., EMBO J 19, 710-718 (2000)). This was an unexpected result as the 2 processes were thought to be very tightly coupled in bacteria. Furthermore, transcription becomes concentrated into a sub-fraction of the bacterial nucleoid at higher growth rates. These transcription foci have been shown to be the sites of rRNA synthesis, and my laboratory is now focussing on characterising transcription complexes and their structure in detail.

Research Expertise
My research career has focused on the gram positive bacterium Bacillus subtilis. During my PhD I purified and characterised the replication terminator protein (RTP) and its DNA binding sites (Lewis et al., J. Bacteriol 171, 3564-3567 (1989); Lewis et al., J. Mol. Biol. 214, 73-84 (1990)). These sites are regions of DNA where oppositely moving replication forks meet and newly replicated chromosomes are resolved prior to segregation. During my post-doctoral research I was responsible for the development of cell biological techniques and vectors for visualising gene expression and protein localisation in live bacterial cells (Lewis et al., PNAS 91, 3849-3853 (1994); Lewis et al., Mol Microbiol 13, 655-662 (1994); Lewis and Errington, Microbiology 142, 733-740 (1996); Lewis and Marston, Gene 227, 101-109 (1999); Feucht and Lewis, Gene 264, 289-297 (2001)). These techniques have been widely adopted by research groups involved in microbial cell biology, and the plasmid vectors have now been distributed directly to well over 100 laboratories world-wide, although they are now also distributed by the Bacillus Genetic Stock Centre. These techniques were instrumental in determining the establishment of compartment specificity of developmentally regulated s-factors during sporulation in B. subtilis (Lewis et al., PNAS 91, 3849-3853 (1994)). I also showed that prespore specific accumulation of the transcription regulator SpoIIAA was responsible for initiation of compartment-specific gene expression during development, that this accumulation was probably due to prespore-specific activity of the phosphatase SpoIIE, and that a programme of proteolysis was initiated following this activation event (Lewis et al., Genes Cell 1, 881-894 (1996); Lewis et al., J. Bacteriol 180, 3276-3284 (1998)). I was also involved in work that showed that the highly conserved SpoIIIE (FtsK) protein is a DNA translocase that moves DNA through a division septum (Wu et al., Genes Dev 9, 1316-1326 (1995)). This was the first example of such a phenomenon, and was a very significant finding as previously it was assumed that DNA was segregated into daughter cells/different compartments prior to division septum formation. Finally, I have shown that transcription and translation are spatially separated within bacteria (Lewis et al., EMBO J 19, 710-718 (2000)). This was an unexpected result as the 2 processes were thought to be very tightly coupled in bacteria. Furthermore, transcription becomes concentrated into a sub-fraction of the bacterial nucleoid at higher growth rates. These transcription foci have been shown to be the sites of rRNA synthesis, and my laboratory is now focussing on characterising transcription complexes and their structure in detail.

Teaching Expertise
Molecular biology, particularly bacterial. Microbiology.

Administrative Expertise
No expertise.



Qualifications

  • Doctor of Philosophy, University of Sydney
  • Bachelor of Science, University of Sheffield - UK

Keywords

  • Microscopy
  • Molecular Biology
  • Molecular Microbiology
  • Transcription regulation

Fields of Research

Code Description Percentage
060199 Biochemistry and Cell Biology not elsewhere classified 50
060499 Genetics not elsewhere classified 15
110899 Medical Microbiology not elsewhere classified 35

Professional Experience

UON Appointment

Title Organisation / Department
Professor University of Newcastle
School of Environmental and Life Sciences
Australia

Academic appointment

Dates Title Organisation / Department
1/07/2005 -  Membership - National Convener Molecular Microbiology Special Interest Group Australian Society for Microbiology
Australia
1/07/2000 -  Senior Lecturer University of Newcastle
School of Environmental and Life Sciences
Australia

Membership

Dates Title Organisation / Department
Member of EU 6th Framework Consortium for A$20M EU 6th Framework BaSysBio Project European Union (EU) Framework Collaboration
Australia
OzReader ARC Biological Sciences Committee
Australia

Awards

Research Award

Year Award
2004 International Short-Term Fellowship
Australian Academy of Sciences

Invitations

Participant

Year Title / Rationale
2007 Invited speaker
Organisation: Conference Description: Invited to speak at SGM 162nd Meeting-Edinburgh International Conference Centre
2007 Reviewer
Organisation: Various Publishers in the field of Environmental and Life Science Description: Have been invited to write reviews for top journals in field (Molecular Microbiology, Microbiology and international Review of Cytology) and 2 book chapters in the last 3 years. Work is also now appearing in Microbiology text books.
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Publications

For publications that are currently unpublished or in-press, details are shown in italics.


Chapter (3 outputs)

Year Citation Altmetrics Link
2012 Doherty GP, Mettrick KA, Grainge IR, Lewis PJ, 'Imaging fluorescent protein fusions in live bacteria', Methods in Microbiology, Academic Press, Kidlington, Ox 107-126 (2012) [B1]
Co-authors Ian Grainge
2008 Lewis PJ, 'Subcellular organisation in bacteria', Bacterial Physiology: A Molecular Approach, Springer, Berlin 1-42 (2008) [B1]
2007 Lewis PJ, 'The organization of transcription and translation', Bacillus: Cellular and Molecular Biology, Caister Academic Press, Norfolk, United Kingdom 135-166 (2007) [B1]

Journal article (50 outputs)

Year Citation Altmetrics Link
2015 Yang X, Ma C, Lewis PJ, 'Identification of inhibitors of bacterial RNA polymerase.', Methods, (2015)
DOI 10.1016/j.ymeth.2015.05.005
2015 Mielczarek M, Thomas RV, Ma C, Kandemir H, Yang X, Bhadbhade M, et al., 'Synthesis and biological activity of novel mono-indole and mono-benzofuran inhibitors of bacterial transcription initiation complex formation', Bioorganic and Medicinal Chemistry, (2015)

Our ongoing research focused on targeting transcription initiation in bacteria has resulted in synthesis of several classes of mono-indole and mono-benzofuran inhibitors that targ... [more]

Our ongoing research focused on targeting transcription initiation in bacteria has resulted in synthesis of several classes of mono-indole and mono-benzofuran inhibitors that targeted the essential protein-protein interaction between RNA polymerase core and s 70/s A factors in bacteria. In this study, the reaction of indole-2-, indole-3-, indole-7- and benzofuran-2-glyoxyloyl chlorides with amines and hydrazines afforded a variety of glyoxyloylamides and glyoxyloylhydrazides. Similarly, condensation of 2- and 7-trichloroacetylindoles with amines and hydrazines delivered amides and hydrazides. The novel molecules were found to inhibit the RNA polymerase-s 70/s A interaction as measured by ELISA, and also inhibited the growth of both Gram-positive and Gram-negative bacteria in culture. Structure-activity relationship (SAR) studies of the mono-indole and mono-benzofuran inhibitors suggested that the hydrophilic-hydrophobic balance is an important determinant of biological activity.

DOI 10.1016/j.bmc.2015.02.037
2015 Mielczarek M, Thomas RV, Ma C, Kandemir H, Yang X, Bhadbhade M, et al., 'Synthesis and biological activity of novel mono-indole and mono-benzofuran inhibitors of bacterial transcription initiation complex formation', Bioorganic and Medicinal Chemistry, 23 1763-1775 (2015)

Our ongoing research focused on targeting transcription initiation in bacteria has resulted in synthesis of several classes of mono-indole and mono-benzofuran inhibitors that targ... [more]

Our ongoing research focused on targeting transcription initiation in bacteria has resulted in synthesis of several classes of mono-indole and mono-benzofuran inhibitors that targeted the essential protein-protein interaction between RNA polymerase core and s70/sA factors in bacteria. In this study, the reaction of indole-2-, indole-3-, indole-7- and benzofuran-2-glyoxyloyl chlorides with amines and hydrazines afforded a variety of glyoxyloylamides and glyoxyloylhydrazides. Similarly, condensation of 2- and 7-trichloroacetylindoles with amines and hydrazines delivered amides and hydrazides. The novel molecules were found to inhibit the RNA polymerase-s70/sA interaction as measured by ELISA, and also inhibited the growth of both Gram-positive and Gram-negative bacteria in culture. Structure-activity relationship (SAR) studies of the mono-indole and mono-benzofuran inhibitors suggested that the hydrophilic-hydrophobic balance is an important determinant of biological activity.

DOI 10.1016/j.bmc.2015.02.037
2015 Ma C, Mobli M, Yang X, Keller AN, King GF, Lewis PJ, 'RNA polymerase-induced remodelling of NusA produces a pause enhancement complex.', Nucleic Acids Res, 43 2829-2840 (2015)
DOI 10.1093/nar/gkv108
2015 Yin Z, Whittell LR, Wang Y, Jergic S, Ma C, Lewis PJ, et al., 'Bacterial Sliding Clamp Inhibitors that Mimic the Sequential Binding Mechanism of Endogenous Linear Motifs.', J Med Chem, 58 4693-4702 (2015)
DOI 10.1021/acs.jmedchem.5b00232
2015 Bi Y, Yang X, Zhang T, Liu Z, Zhang X, Lu J, et al., 'Design, synthesis, nitric oxide release and antibacterial evaluation of novel nitrated ocotillol-type derivatives.', Eur J Med Chem, 101 71-80 (2015)
DOI 10.1016/j.ejmech.2015.06.021
2015 Bi Y, Ma C, Zhou Z, Zhang T, Zhang H, Zhang X, et al., 'Synthesis and antibacterial evaluation of novel hydrophilic ocotillol-type triterpenoid derivatives from 20(S)-protopanaxadiol', Records of Natural Products, 9 356-368 (2015)

Triterpenoid saponins are involved in plant defense systems to inhibit bacterial invasion. A new series of hydrophilic ocotillol-type triterpenoid derivatives 5-26 have been synth... [more]

Triterpenoid saponins are involved in plant defense systems to inhibit bacterial invasion. A new series of hydrophilic ocotillol-type triterpenoid derivatives 5-26 have been synthesized with antibacterial activity against Gram-positive bacteria, including a community associated methicillin-resistant Staphylococcus aureus (CA-MRSA; strain USA300). From this series, compounds 6 and 15 were found to be the most active, both with MIC values of 2 µg/mL against B. subtilis 168 and 8 µg/mL against S. aureus USA300, respectively. Furthermore, subsequent assays showed that compounds 6 and 15 displayed strong synergistic effects at sub-MIC levels against both S. aureus USA300 and B. subtilis 168 when combined with two commercial antibiotics, kanamycin and chloramphenicol. Preliminary structure-activity relationship studies were also performed.

2014 Keller AN, Yang X, Wiedermannová J, Delumeau O, Krásný L, Lewis PJ, 'e, a new subunit of RNA polymerase found in gram-positive bacteria.', J Bacteriol, 196 3622-3632 (2014) [C1]
DOI 10.1128/JB.02020-14
Citations Scopus - 1Web of Science - 1
2014 Withers R, Doherty GP, Jordan M, Yang X, Dixon NE, Lewis PJ, 'AtfA, a new factor in global regulation of transcription in Acinetobacter spp', Molecular Microbiology, 93 1130-1143 (2014) [C1]

Acinetobacter species are widely distributed bacteria in the environment, and have recently gained notoriety as opportunistic nosocomial pathogens. Here we characterize a novel RN... [more]

Acinetobacter species are widely distributed bacteria in the environment, and have recently gained notoriety as opportunistic nosocomial pathogens. Here we characterize a novel RNA polymerase-interacting protein named acidic transcription factor A, AtfA. It is small and highly acidic, and is widely distributed throughout the ¿ proteobacteria, including other significant pathogens in the genera Moraxella, Pseudomonas, Legionella and Vibrio. In the model species A. baylyiADP1, deletion of atfA significantly affects expression of over 500 genes, resulting in a large cell phenotype, reduced cell fitness, impaired biofilm formation and twitching motility, and increased sensitivity to antibiotics. Deletion of atfA also causes dramatically enhanced sensitivity to ethanol, which is an important growth promoter and virulence factor in Acinetobacter spp. The results suggest that auxiliary factors of RNA polymerase with important biological roles remain to be discovered.

DOI 10.1111/mmi.12723
Citations Scopus - 1Web of Science - 1
2014 Yang X, Ma C, Lewis P, 'A vector system that allows simple generation of mutant Escherichia coli RNA polymerase', PLASMID, 75 37-41 (2014) [C1]
DOI 10.1016/j.plasmid.2014.06.002
Citations Scopus - 1Web of Science - 1
2014 Yang X, Ma C, Lewis P, 'A vector system that allows simple generation of mutant Escherichia coli RNA polymerase', Plasmid, 75 37-41 (2014) [C1]

We describe a dual vector-based system for overproduction of recombinant Escherichia coli RNA polymerase (RNAP). A cleavable deca-histidine tag (His10) was incorporated into the C... [more]

We describe a dual vector-based system for overproduction of recombinant Escherichia coli RNA polymerase (RNAP). A cleavable deca-histidine tag (His10) was incorporated into the C-terminus of the ß' subunit to facilitate protein purification. Unique restriction sites were introduced into the genes encoding the ß and ß' subunits (rpoB and rpoC, respectively), facilitating mutation of functionally significant subunit fragments through insertion of modified PCR fragments into the appropriate vector. RNAP with an R275A substitution in the ß' subunit, which is essential for interaction with transcription initiation factor s, was generated and exhibited reduced activity compared to native recombinant RNAP.

DOI 10.1016/j.plasmid.2014.06.002
2014 Withers R, Doherty GP, Jordan M, Yang X, Dixon NE, Lewis PJ, 'AtfA, a new factor in global regulation of transcription in Acinetobacter spp.', MOLECULAR MICROBIOLOGY, 93 1130-1143 (2014) [C1]
DOI 10.1111/mmi.12723
Citations Web of Science - 1
2014 Withers R, Doherty GP, Jordan M, Yang X, Dixon NE, Lewis PJ, 'AtfA, a new factor in global regulation of transcription in Acinetobacter spp', Molecular Microbiology, 93 1130-1143 (2014) [C1]

Acinetobacter species are widely distributed bacteria in the environment, and have recently gained notoriety as opportunistic nosocomial pathogens. Here we characterize a novel RN... [more]

Acinetobacter species are widely distributed bacteria in the environment, and have recently gained notoriety as opportunistic nosocomial pathogens. Here we characterize a novel RNA polymerase-interacting protein named acidic transcription factor A, AtfA. It is small and highly acidic, and is widely distributed throughout the ¿ proteobacteria, including other significant pathogens in the genera Moraxella, Pseudomonas, Legionella and Vibrio. In the model species A. baylyiADP1, deletion of atfA significantly affects expression of over 500 genes, resulting in a large cell phenotype, reduced cell fitness, impaired biofilm formation and twitching motility, and increased sensitivity to antibiotics. Deletion of atfA also causes dramatically enhanced sensitivity to ethanol, which is an important growth promoter and virulence factor in Acinetobacter spp. The results suggest that auxiliary factors of RNA polymerase with important biological roles remain to be discovered.

DOI 10.1111/mmi.12723
Citations Scopus - 1
2014 Kandemir H, Kutty SK, Black DS, Kumar N, Ma C, Lewis PJ, Griffith R, 'Synthesis and biological evaluation of 2,5-di(7-indolyl)-1,3,4-oxadiazoles, and 2- and 7-indolyl 2-(1,3,4-thiadiazolyl)ketones as antimicrobials', Bioorganic and Medicinal Chemistry, (2014) [C1]

A range of novel hydrazine bridged bis-indoles was prepared from readily available indole-7-glyoxyloylchlorides and 7-trichloroacetylindoles and underwent cyclodehydration to prod... [more]

A range of novel hydrazine bridged bis-indoles was prepared from readily available indole-7-glyoxyloylchlorides and 7-trichloroacetylindoles and underwent cyclodehydration to produce 2,5-di(7-indolyl)-1,3,4-oxadiazoles and a 2,2'-bi-1,3,4-oxadiazolyl with phosphoryl chloride in ethyl acetate. This efficient protocol was subsequently used for the synthesis of 2- and 7-indolyl 2-(1,3,4-thiadiazolyl)ketones from related indolyl-hydrazine carbothioamides. The synthesised bis-indoles were evaluated for their antimicrobial properties, particularly the inhibition of protein-protein complex formation between RNA polymerase and s factor and their bactericidal effect on Gram positive Bacillus subtilis and Gram negative Escherichia coli. © 2014 Elsevier Ltd. All rights reserved.

DOI 10.1016/j.bmc.2014.01.025
Citations Scopus - 1Web of Science - 1
2014 Mielczarek M, Devakaram RV, Ma C, Yang X, Kandemir H, Purwono B, et al., 'Synthesis and biological activity of novel bis-indole inhibitors of bacterial transcription initiation complex formation', Organic and Biomolecular Chemistry, 12 2882-2894 (2014) [C1]

The increasing resistance of bacteria against clinically approved antibiotics is resulting in an alarming decrease in therapeutic options for today's clinicians. We have targeted ... [more]

The increasing resistance of bacteria against clinically approved antibiotics is resulting in an alarming decrease in therapeutic options for today's clinicians. We have targeted the essential interaction between bacterial RNA polymerase and s70/sA for the development of lead molecules exhibiting a novel mechanism of antibacterial activity. Several classes of structurally related bis-indole inhibitors of bacterial transcription initiation complex formation were synthesized and their antimicrobial activities were evaluated. Condensation of indole-7- and indole-2-carbohydrazides with 7- and 2-trichloroacetylindoles or indole-7- and indole-2-glyoxyloyl chlorides resulted in the successful synthesis of 7,7'-, 2,2'-, 2,7'- and 3,2'-linked bis-indole derivatives with -CO-NH-NH-CO- and -CO-CO-NH-NH-CO- linkers. Indole-7-glyoxyloyl chlorides were reacted with hydrazine hydrate in different ratios to afford respective -CO-CO-NH-NH-CO-CO- bis-indole or hydrazide derivatives. The resulting compounds were found to be active against the ß'-CH- s70/sA2.2 interaction in ELISA assays and inhibited the growth of both Gram-positive and Gram-negative bacteria. Structure-activity relationship (SAR) studies were performed in order to identify the structural features of the synthesized inhibitors required for biological activity. This journal is © the Partner Organisations 2014.

DOI 10.1039/c4ob00460d
Citations Scopus - 5Web of Science - 5
2014 Bi Y, Ma C, Zhang H, Zhou Z, Yang J, Zhang Z, et al., 'Novel 3-Substituted Ocotillol-Type Triterpenoid Derivatives as Antibacterial Candidates', CHEMICAL BIOLOGY & DRUG DESIGN, 84 489-496 (2014) [C1]
DOI 10.1111/cbdd.12337
Citations Scopus - 1Web of Science - 1
2013 Zhou Z, Ma C, Zhang H, Bi Y, Chen X, Tian H, et al., 'Synthesis and biological evaluation of novel ocotillol-type triterpenoid derivatives as antibacterial agents', European Journal of Medicinal Chemistry, 68 444-453 (2013) [C1]
DOI 10.1016/j.ejmech.2013.07.041
Citations Scopus - 4Web of Science - 3
2013 Ma C, Yang X, Kandemir H, Mielczarek M, Johnston EB, Griffith R, et al., 'Inhibitors of Bacterial Transcription Initiation Complex Formation', ACS Chemical Biology, 8 1972-1980 (2013) [C1]
DOI 10.1021/cb400231p
Citations Scopus - 13Web of Science - 11
2013 Brzoska AJ, Hassan KA, de Leon EJ, Paulsen IT, Lewis PJ, 'Single-Step Selection of Drug Resistant Acinetobacter baylyi ADP1 Mutants Reveals a Functional Redundancy in the Recruitment of Multidrug Efflux Systems', PLOS ONE, 8 (2013) [C1]
DOI 10.1371/journal.pone.0056090
Citations Scopus - 5Web of Science - 5
2012 Buescher JM, Liebermeister W, Jules M, Uhr M, Muntel J, Botella E, et al., 'Global network reorganization during dynamic adaptations of Bacillus subtilis metabolism', Science, 335 1099-1103 (2012) [C1]
DOI 10.1126/science.1206871
Citations Scopus - 88Web of Science - 84
2012 Nicolas P, Mader U, Dervyn E, Rochat T, Leduc A, Pigeonneau N, et al., 'Condition-dependent transcriptome reveals high-level regulatory architecture in Bacillus subtilis', Science, 335 1103-1106 (2012) [C1]
Citations Scopus - 165
2012 Buncherd H, Nessen MA, Nouse N, Stelder SK, Roseboom W, Dekker HL, et al., 'Selective enrichment and identification of cross-linked peptides to study 3-D structures of protein complexes by mass spectrometry', Journal of Proteomics, 75 2205-2215 (2012) [C1]
DOI 10.1016/j.jprot.2012.01.025
Citations Scopus - 7Web of Science - 8
2011 Liew ATF, Theis T, Jensen SO, Garcia-Lara J, Foster SJ, Firth N, et al., 'A simple plasmid-based system that allows rapid generation of tightly controlled gene expression in Staphylococcus aureus', Microbiology, 157 666-676 (2011) [C1]
DOI 10.1099/mic.0.045146-0
Citations Scopus - 10Web of Science - 10
2010 Yang X, Lewis PJ, 'The interaction between RNA polymerase and the elongation factor NusA', RNA Biology, 7 272-275 (2010) [C1]
Citations Scopus - 8Web of Science - 9
2010 Yang X, Lewis PJ, 'The interaction between bacterail transcription factors and RNA polymerase during the transition from initiation to elongation', Transcription, 1 1-4 (2010) [C1]
DOI 10.4161/trns.1.2.12791
Citations Scopus - 4
2010 Doherty GP, Bailey KL, Lewis PJ, 'Stage-specific fluorescence intensity of GFP and mCherry during sporulation in Bacillus Subtilis', BMC Research Notes, 3 1-8 (2010) [C1]
DOI 10.1186/1756-0500-3-303
Citations Scopus - 9
2010 Robinson A, Brzoska AJ, Turner KM, Withers RW, Harry EJ, Lewis PJ, Dixon NE, 'Essential biological processes of an emerging pathogen: DNA replication, transcription, and cell division in Acinetobacter spp', Microbiology and Molecular Biology Reviews, 74 273-297 (2010) [C1]
DOI 10.1128/MMBR.00048-09
Citations Scopus - 21Web of Science - 24
2010 Botella E, Fogg M, Jules M, Piersma S, Doherty GP, Hansen A, et al., 'pBaSysBioII: An integrative plasmid generating gfp transcriptional fusions for high-throughput analysis of gene expression in Bacillus subtilis', Microbiology, 156 1600-1608 (2010) [C1]
DOI 10.1099/mic.0.035758-0
Citations Scopus - 29Web of Science - 29
2010 Doherty GP, Fogg MJ, Wilkinson AJ, Lewis PJ, 'Small subunits of RNA polymerase: Localization, levels and implications for core enzyme composition', Microbiology, 16 3532-3543 (2010) [C1]
DOI 10.1099/mic.0.041566-0
Citations Scopus - 14Web of Science - 14
2009 Yang X, Molimau S, Doherty GP, Johnston EB, Marles-Wright J, Rothnagel R, et al., 'The structure of bacterial RNA polymerase in complex with the essential transcription elongation factor NusA', EMBO Reports, 10 997-1002 (2009) [C1]
DOI 10.1038/embor.2009.155
Citations Scopus - 25Web of Science - 25
2009 Johnston EB, Lewis PJ, Griffith R, 'The interaction of Bacillus subtilis sigma(A) with RNA polymerase', Protein Science, 18 2287-2297 (2009) [C1]
DOI 10.1002/pro.239
Citations Scopus - 13Web of Science - 13
2008 Yang X, Lewis PJ, 'Overproduction and purification of recombinant Bacillus subtilis RNA polymerase', Protein Expression and Purification, 59 86-93 (2008) [C1]
DOI 10.1016/j.pep.2008.01.006
Citations Scopus - 9Web of Science - 11
2008 Meredith DH, Plank MW, Lewis PJ, 'Different patterns of integral membrane protein localization during cell division in Bacillus subtilis', Microbiology, 154 64-71 (2008) [C1]
DOI 10.1099/mic.0.2007/013268-0
Citations Scopus - 7Web of Science - 6
2008 Lewis PJ, Doherty GP, Clarke J, 'Transcription factor dynamics', Microbiology, 154 1837-1844 (2008) [C1]
DOI 10.1099/mic.0.2008/018549-0
Citations Scopus - 5Web of Science - 6
2008 Huo N, Lewis PJ, 'Green fluorescent protein labeling of ylyA gene in Bacillus subtilis', Weishengwu Xuebao, 48 829-833 (2008) [C1]
2008 Marles-Wright J, Grant T, Delumeau O, Van Duinen G, Firbank SJ, Lewis PJ, et al., 'Molecular architecture of the 'stressosome,' a signal integration and transduction hub', Science, 322 92-96 (2008) [C1]
DOI 10.1126/science.1159572
Citations Scopus - 67Web of Science - 63
2008 Yang X, Doherty GP, Lewis PJ, 'Tandem affinity purification vectors for use in gram positive bacteria', Plasmid, 59 54-62 (2008) [C1]
DOI 10.1016/j.plasmid.2007.11.001
Citations Scopus - 2Web of Science - 2
2006 Doherty GP, Meredith DH, Lewis PJ, 'Subcellular partitioning of transcription factors in Bacillus subtilis', Journal of Bacteriology, 188 4101-4110 (2006) [C1]
DOI 10.1128/JB.01934-05
Citations Scopus - 11Web of Science - 12
2005 Davies KM, Dedman AJ, van Horck S, Lewis PJ, 'The NusA:RNA polymerase ratio is increased at sites of rRNA synthesis in Bacillus subtilis.', Mol Microbiol, 57 366-379 (2005)
DOI 10.1111/j.1365-2958.2005.04469.x
2005 MacDougall IJA, Lewis PJ, Griffith R, 'Homology modelling of RNA polymerase and associated transcription factors from Bacillus subtilis', Journal of Molecular Graphics & Modelling, 23 297-303 (2005) [C1]
DOI 10.1016/j.jmgm.2004.10.001
Citations Scopus - 5Web of Science - 6
2005 Davies KM, Dedman AJ, Van Horck SH, Lewis PJ, 'The NusA : RNA polymerase ratio is increased at sites of rRNA synthesis in Bacillus subtilis', Molecular Microbiology, 57 366-379 (2005) [C1]
DOI 10.1111/j.1365-2958.2005.04669.x
Citations Scopus - 20Web of Science - 20
2004 Migocki MD, Lewis PJ, Wake RG, Harry EJ, 'The midcell replication factory in Bacillus subtilis is highly mobile: implications for coordinating chromosome replication with other cell cycle events', Molecular Microbiology, 54 452-463 (2004) [C1]
DOI 10.1111/j.1365-2958.2004.04267.x
Citations Scopus - 38Web of Science - 41
2004 Lewis PJ, 'Bacterial subcellular architecture: recent advances and future prospects', Molecular Microbiology, 54 1135-1150 (2004) [C1]
DOI 10.1111/j.1365-2958.2004.04343.x
Citations Scopus - 30Web of Science - 32
2004 Johnson AS, Van Horck SH, Lewis PJ, 'Dynamic localization of membrance proteins in Bacillus subtilis', Microbiology, 150 2815-2824 (2004) [C1]
DOI 10.1099/mic.0.27223-0
Citations Scopus - 32Web of Science - 34
2003 Davies KM, Lewis PJ, 'Localization of rRNA Synthesis in Bacillus subtilis: Characterization of Loci Involved in Transcription Focus Formation', Journal of Bacteriology, 185 2346-2353 (2003) [C1]
DOI 10.1128/JB.185.7.2346-2353.2003
Citations Scopus - 16Web of Science - 16
2003 Harry EJ, Lewis PJ, 'Early targeting of Min proteins to the cell poles in germinated spores of Bacillus subtilis: evidence for division apparatus-independent recruitment of Min proteins to the division site', Molecular Microbiology, 47 37-48 (2003) [C1]
DOI 10.1046/j.1365-2958.2003.03253.x
Citations Scopus - 40Web of Science - 41
2001 Lewis PJ, 'Bacterial chromosome segregation', Microbiology, 147 519-526 (2001) [C1]
Citations Scopus - 18Web of Science - 19
2001 Feucht A, Lewis PJ, 'Improved plasmid vectors for the production of multiple fluorescent protein fusions in Bacillus subtilis', Gene, 264 289-297 (2001) [C1]
Citations Scopus - 45Web of Science - 45
2000 Meijer WJJ, Lewis PJ, Errington J, Salas M, 'Dynamic relocalization of phage f29 DNA during replication and the role of the viral protein p16.7', EMBO Journal, 19 4182-4190 (2000)

We have examined the localization of DNA replication of the Bacillus subtilis phage f29 by immunofluorescence. To determine where phage replication was localized within infected c... [more]

We have examined the localization of DNA replication of the Bacillus subtilis phage f29 by immunofluorescence. To determine where phage replication was localized within infected cells, we examined the distribution of phage replication proteins and the sites of incorporation of nucleotide analogues into phage DNA. On initiation of replication, the phage DNA localized to a single focus within the cell, nearly always towards one end of the host cell nucleoid. At later stages of the infection cycle, phage replication was found to have redistributed to multiple sites around the periphery of the nucleoid, just under the cell membrane. Towards the end of the cycle, phage DNA was once again redistributed to become located within the bulk of the nucleoid. Efficient redistribution of replicating phage DNA from the initial replication site to various sites surrounding the nucleoid was found to be dependent on the phage protein p16.7.

Citations Scopus - 18
1994 LEWIS PJ, NWOGUH CE, BARER MR, HARWOOD CR, ERRINGTON J, 'USE OF DIGITIZED VIDEO MICROSCOPY WITH A FLUOROGENIC ENZYME-SUBSTRATE TO DEMONSTRATE CELL-SPECIFIC AND COMPARTMENT-SPECIFIC GENE-EXPRESSION IN SALMONELLA-ENTERITIDIS AND BACILLUS-SUBTILIS', MOLECULAR MICROBIOLOGY, 13 655-662 (1994)
DOI 10.1111/j.1365-2958.1994.tb00459.x
Citations Web of Science - 30
Show 47 more journal articles

Conference (21 outputs)

Year Citation Altmetrics Link
2008 Brzoska AJ, Turner K, Robinson A, Dixon N, Harry L, Lewis PJ, 'Identification of novel protein-protein interactions in Acinetobacter spp', ASM 2008 Melbourne: Program and Abstracts Book, Melbourne, VIC (2008) [E3]
2008 Robinson A, Brzoska AJ, Turner K, Harry L, Lewis PJ, Dixon N, 'DNA replication in pathogenic bacteria: Understanding and disrupting protein-protein interactions', ASM 2008 Melbourne: Program and Abstracts Book, Melbourne, VIC (2008) [E3]
2008 Turner K, Brzoska AJ, Robinson A, Firth N, Lewis PJ, Dixon N, Harry L, 'Isolation of novel cell division complexes using tandem affinity purification', ASM 2008 Melbourne: Program and Abstracts Book, Melbourne, VIC (2008) [E3]
2008 Withers RW, Brzoska AJ, Lewis PJ, 'Establishment of an in vitro transcription assay for Acinetobacter spp', ASM 2008 Melbourne: Program and Abstracts Book, Melbourne, VIC (2008) [E3]
2008 Brzoska AJ, Lewis PJ, 'Identification of novel protein-protein interactions in S. aureus', International Symposium on Staphylococci and Staphylococcal Infections: Abstracts, Cairns, QLD (2008) [E3]
2008 Lewis PJ, 'Subcellular localization of transcription factors', Society for General Microbiology Spring Meeting Abstracts, Edinburgh, Scotland (2008) [E3]
2007 Johnston EB, Griffith R, Lewis PJ, 'The design and testing of inhibitors of interactions between essential transcription factors and RNA polymerase', 32nd Lorne Conference on Protein Structure and Function. Abstracts, Lorne, Vic (2007) [E3]
2007 Lewis PJ, Meredith DH, Plank MW, 'Selective removal of integral membrane proteins from the mid-cell site at the onset of cell division', 4th Conference on Functional Genomics of Gram-Positive Microorganisms. Abstracts, Tirrenia, Pisa, Italy (2007) [E3]
2007 Yang X, Lewis PJ, 'Structure of transcription factor NUSA-RNA polymerase complex', ASM 2007 Adelaide Program and Abstracts Book, Adelaide, SA (2007) [E3]
2007 Lewis PJ, Doherty GP, Meredith DH, 'Transcription factor dynamics', ASM 2007 Adelaide Program and Abstracts Book, Adelaide, SA (2007) [E3]
2007 Harriott K, Lewis PJ, 'Characterisation of the interaction between PcrA and the b sub-unit of RNA polymerase', BacPath 9 : The Molecular Biology of Bacterial Pathogens. Abstracts, Lorne, VIC (2007) [E3]
2007 Johnston EB, Griffith R, Lewis PJ, 'The design and testing of inhibitors of interactions between essential transcription factors and RNA polymerase', BacPath 9 : The Molecular Biology of Bacterial Pathogens. Abstracts, Lorne, VIC (2007) [E3]
2004 MacDougall IJA, Lewis PJ, Griffith R, 'Molecular modelling of the RNA polymerase complex in Bacillus subtilis', MM2004: 9th Molecular Modelling Workshop, Sydney, Australia (2004) [E3]
2004 Lewis PJ, 'Subcellular organization of transcription in bacteria', ASM 2004 Sydney National Conference, Sydney, Australia (2004) [E3]
2004 Doherty GP, Meredith DH, Lewis PJ, 'Composition of Transcription complexes in Bacillus subtilis', ASM 2004 Sydney National Conference, Sydney, Australia (2004) [E3]
2004 Martin S, MacDougall IJA, Griffith R, Lewis PJ, 'Investigation of the interactions between transcription factor NusA with RNA polymerase in Bacillus subtilis', ASM 2004 Sydney National Conference, Sydney, Australia (2004) [E3]
2004 Migocki MD, Lewis PJ, Wake G, Harry E, 'Is chromosome replication involved in the spatial regulation of midcell division in Bacillus subtilis?', ASM 2004 Sydney National Conference, Sydney, Australia (2004) [E3]
2003 Davies KM, Dedman A, Walsh AK, Van Horck SH, Lewis PJ, 'The bacterial transcription factor NusA is preferentially recruited to sites of rRNA synthesis: Direct evidence for differential RNAP elongation complex formation in vivo', Functional Genomics of Gram-positive Microorganisms 12th International Conference on Bacilli, Baveno, Italy (2003) [E3]
2002 Lewis PJ, Davies KM, 'Analysis of transcription foci in bacillus subtilis', American Society for Microbiology - ASM Conference: Prokaryotic Development, Quebec, CANADA (2002) [E3]
2002 Lewis PJ, Harry E, 'Cellular targeting of proteins involved in positioning the division site in bacteria', Proceedings of the Australian Society for Biochemistry and Molecular Biology, Sydney, NSW (2002) [E3]
2001 Lewis PJ, 'Compartmentalisation of transcription and translation in bacteria', Australian Society for Biochemistry & Molecular Biology Incorp - Combio 2001, Canberra ACT (2001) [E3]
Show 18 more conferences
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Grants and Funding

Summary

Number of grants 45
Total funding $26,063,564

Click on a grant title below to expand the full details for that specific grant.


20141 grants / $20,000

Development of compounds that inhibit transcription initiation $20,000

Funding body: University of Newcastle

Funding body University of Newcastle
Project Team Professor Peter Lewis
Scheme Near Miss Grant
Role Lead
Funding Start 2014
Funding Finish 2014
GNo G1301396
Type Of Funding Internal
Category INTE
UON Y

20133 grants / $53,000

Characterisation of a macromolecular complex essential for rapid microbial cell proliferation$20,000

Funding body: University of Newcastle

Funding body University of Newcastle
Project Team Professor Peter Lewis, Doctor Xiao Yang
Scheme Near Miss Grant
Role Lead
Funding Start 2013
Funding Finish 2013
GNo G1300482
Type Of Funding Internal
Category INTE
UON Y

Characterisation of a macromolecular complex essential for rapid microbial cell proliferation$20,000

Funding body: Hunter Medical Research Institute

Funding body Hunter Medical Research Institute
Project Team Professor Peter Lewis
Scheme Near Miss
Role Lead
Funding Start 2013
Funding Finish 2013
GNo G1300698
Type Of Funding External
Category EXTE
UON Y

New Drugs to Kill Superbugs$13,000

Funding body: University of Newcastle - Faculty of Science & IT

Funding body University of Newcastle - Faculty of Science & IT
Project Team Professor Peter Lewis, Professor Adam McCluskey, Doctor Chris Gordon, Doctor Joey Ambrus
Scheme Strategic Small Grant
Role Lead
Funding Start 2013
Funding Finish 2013
GNo G1401065
Type Of Funding Internal
Category INTE
UON Y

20123 grants / $323,426

Targeting nucleic acid synthesis and cell division in gram-negative bacterial pathogens$311,439

Funding body: NHMRC (National Health & Medical Research Council)

Funding body NHMRC (National Health & Medical Research Council)
Project Team Professor Nicholas Dixon, Associate Professor Elizabeth Harry, Professor Peter Lewis, Associate Professor Aaron Oakley, Doctor Ian Grainge
Scheme Project Grant
Role Lead
Funding Start 2012
Funding Finish 2012
GNo G1101133
Type Of Funding Aust Competitive - Commonwealth
Category 1CS
UON Y

Ultrasonic Homogenizer System and -80 ºC Freezers for chemical and biological sample storage$10,000

Funding body: NHMRC (National Health & Medical Research Council)

Funding body NHMRC (National Health & Medical Research Council)
Project Team Professor Adam McCluskey, Professor Eileen McLaughlin, Professor Peter Lewis, Ms Belinda Nixon, Doctor Shaun Roman, Doctor Jennette Sakoff, Doctor Ian Grainge
Scheme Equipment Grant
Role Investigator
Funding Start 2012
Funding Finish 2012
GNo G1100986
Type Of Funding Other Public Sector - Commonwealth
Category 2OPC
UON Y

Faculty Visiting Fellowship 2012$1,987

Funding body: University of Newcastle - Faculty of Science & IT

Funding body University of Newcastle - Faculty of Science & IT
Project Team Professor Peter Lewis
Scheme Visiting Fellowship
Role Lead
Funding Start 2012
Funding Finish 2012
GNo G1401126
Type Of Funding Internal
Category INTE
UON Y

20116 grants / $2,011,667

Exploitation of bacterial transcription initiation as a target for new antimicrobials$521,706

Funding body: NHMRC (National Health & Medical Research Council)

Funding body NHMRC (National Health & Medical Research Council)
Project Team Professor Peter Lewis, Associate Professor Renate Griffith, Associate Professor Naresh Kumar
Scheme Project Grant
Role Lead
Funding Start 2011
Funding Finish 2011
GNo G1000285
Type Of Funding Aust Competitive - Commonwealth
Category 1CS
UON Y

Establishment of a comprehensive regional biophysical analysis facility $500,000

Funding body: ARC (Australian Research Council)

Funding body ARC (Australian Research Council)
Project Team Professor Peter Lewis, Professor Nicholas Dixon, Professor Eileen McLaughlin, Professor Adam McCluskey, Associate Professor Aaron Oakley, Laureate Professor Paul Foster, Laureate Professor John Aitken, Associate Professor Jennifer Beck, Dr Michael Kelso, Professor Graham Nicholson, Associate Professor Elizabeth Harry, Professor Ian Charles, Associate Professor Steven Djordjevic
Scheme Linkage Infrastructure Equipment & Facilities (LIEF)
Role Lead
Funding Start 2011
Funding Finish 2011
GNo G1000630
Type Of Funding Scheme excluded from IGS
Category EXCL
UON Y

Chemical Biology$444,961

Funding body: University of Newcastle

Funding body University of Newcastle
Project Team Professor Adam McCluskey, Doctor Warwick Belcher, Doctor Ian Grainge, Professor Christopher Grof, Professor Peter Lewis, Professor Eileen McLaughlin, Doctor Shaun Roman, Conjoint Professor Ray Rose, Doctor Jennette Sakoff, Doctor Nikki Verrills
Scheme Priority Research Centre
Role Investigator
Funding Start 2011
Funding Finish 2011
GNo G1100052
Type Of Funding Internal
Category INTE
UON Y

Structure function analysis of the NusA-RNA polymerase interaction$300,000

Funding body: ARC (Australian Research Council)

Funding body ARC (Australian Research Council)
Project Team Professor Peter Lewis, Doctor Xiao Yang, Dr Richard Lewis, Dr Jon Marles-Wright
Scheme Discovery Projects
Role Lead
Funding Start 2011
Funding Finish 2011
GNo G1000129
Type Of Funding Aust Competitive - Commonwealth
Category 1CS
UON Y

Establishment of a comprehensive regional biophysical analysis facility $230,000

Funding body: ARC (Australian Research Council)

Funding body ARC (Australian Research Council)
Project Team Professor Peter Lewis, Professor Nicholas Dixon, Professor Eileen McLaughlin, Professor Adam McCluskey, Associate Professor Aaron Oakley, Laureate Professor Paul Foster, Laureate Professor John Aitken, Associate Professor Jennifer Beck, Dr Michael Kelso, Professor Graham Nicholson, Associate Professor Elizabeth Harry, Professor Ian Charles, Associate Professor Steven Djordjevic
Scheme Linkage Infrastructure Equipment & Facilities (LIEF) Partner funding
Role Lead
Funding Start 2011
Funding Finish 2011
GNo G1100484
Type Of Funding Scheme excluded from IGS
Category EXCL
UON Y

Eppendorf mastercycler pro with thermomixer comfort and 5430R centrifuge$15,000

Funding body: NHMRC (National Health & Medical Research Council)

Funding body NHMRC (National Health & Medical Research Council)
Project Team Professor Eileen McLaughlin, Professor Peter Lewis, Professor Adam McCluskey, Conjoint Professor Keith Jones, Associate Professor Brett Nixon, Doctor Shaun Roman, Doctor Jennette Sakoff, Doctor Ian Grainge, Doctor Janet Holt, Doctor Xiao Yang
Scheme Equipment Grant
Role Investigator
Funding Start 2011
Funding Finish 2011
GNo G1100028
Type Of Funding Other Public Sector - Commonwealth
Category 2OPC
UON Y

20093 grants / $570,000

LAS-4000 Chemiluminescence imaging system$25,000

Funding body: NHMRC (National Health & Medical Research Council)

Funding body NHMRC (National Health & Medical Research Council)
Project Team Professor Eileen McLaughlin, Laureate Professor John Aitken, Professor Peter Lewis, Associate Professor Brett Nixon, Doctor Shaun Roman
Scheme Equipment Grant
Role Investigator
Funding Start 2009
Funding Finish 2009
GNo G0189852
Type Of Funding Other Public Sector - Commonwealth
Category 2OPC
UON Y

20082 grants / $107,600

X-ray crystallography resource for membrane proteins and large macromolecular complexes$93,000

Funding body: ARC (Australian Research Council)

Funding body ARC (Australian Research Council)
Project Team Professor Peter Lewis, Doctor Robyn Malby
Scheme Linkage Infrastructure Equipment & Facilities (LIEF)
Role Lead
Funding Start 2008
Funding Finish 2008
GNo G0188894
Type Of Funding Scheme excluded from IGS
Category EXCL
UON Y

Structural Analysis of Bacterial Transcription Proteins by X-ray Crystallography$14,600

Funding body: University of Newcastle

Funding body University of Newcastle
Project Team Doctor Robyn Malby, Professor Peter Lewis
Scheme Pilot Grant
Role Investigator
Funding Start 2008
Funding Finish 2008
GNo G0189075
Type Of Funding Internal
Category INTE
UON Y

20077 grants / $1,367,901

Nucleic Acid Synthesis and Cell Division in Model Pathogenic Bacteria$740,250

Funding body: NHMRC (National Health & Medical Research Council)

Funding body NHMRC (National Health & Medical Research Council)
Project Team Professor Peter Lewis, Professor Nicholas Dixon, Associate Professor Elizabeth Harry
Scheme Project Grant
Role Lead
Funding Start 2007
Funding Finish 2007
GNo G0186390
Type Of Funding Aust Competitive - Commonwealth
Category 1CS
UON Y

Towards an understanding of dynamic genetic regulation at global scale in bacteria: A systems biology approach$486,000

Funding body: NHMRC (National Health & Medical Research Council)

Funding body NHMRC (National Health & Medical Research Council)
Project Team Professor Peter Lewis
Scheme NHMRC - European Union Collaborative Research Grants
Role Lead
Funding Start 2007
Funding Finish 2007
GNo G0186507
Type Of Funding Aust Competitive - Commonwealth
Category 1CS
UON Y

Towards an understanding of dynamic transcriptional regulation at a global scale in bacteria: a system biology approach$110,250

Funding body: Department of Innovation, Industry, Science and Research

Funding body Department of Innovation, Industry, Science and Research
Project Team Professor Peter Lewis
Scheme International Science Linkages (ISL) Program
Role Lead
Funding Start 2007
Funding Finish 2007
GNo G0187015
Type Of Funding Aust Competitive - Commonwealth
Category 1CS
UON Y

RNA POLYMERASE PROTEIN INTERACTIONS AND THE REGULATION OF TRANSCRIPTION ELONGATION$16,900

Funding body: University of Newcastle

Funding body University of Newcastle
Project Team Professor Peter Lewis
Scheme Near Miss Grant
Role Lead
Funding Start 2007
Funding Finish 2007
GNo G0187186
Type Of Funding Internal
Category INTE
UON Y

Hamamatsu ORCA-AG cooled CCD camera$12,000

Funding body: NHMRC (National Health & Medical Research Council)

Funding body NHMRC (National Health & Medical Research Council)
Project Team Professor Peter Lewis
Scheme Equipment Grant
Role Lead
Funding Start 2007
Funding Finish 2007
GNo G0188190
Type Of Funding Other Public Sector - Commonwealth
Category 2OPC
UON Y

4th Conference on Functional Genomics of Gram-Positive Microorganisms, Green Park Resort, Tirrenia, Italy, 24/6/2007 - 28/6/2007$2,500

Funding body: University of Newcastle

Funding body University of Newcastle
Project Team Professor Peter Lewis
Scheme Travel Grant
Role Lead
Funding Start 2007
Funding Finish 2007
GNo G0187783
Type Of Funding Internal
Category INTE
UON Y

Towards an understanding of dynamic transcriptional regulation at global scale in bacteria: a systems biology approach. BaSysBio$1

Funding body: European Commission, European Union

Funding body European Commission, European Union
Project Team Professor Peter Lewis
Scheme Sixth Framework Programme
Role Lead
Funding Start 2007
Funding Finish 2007
GNo G0186876
Type Of Funding Not Known
Category UNKN
UON Y

20062 grants / $20,273,000

Towards and understanding of dynamic genetic regulation at a global scale in bacteria: a Systems biology approach$20,000,000

Funding body: European Commission, European Union

Funding body European Commission, European Union
Project Team
Scheme 6th Framework
Role Investigator
Funding Start 2006
Funding Finish 2007
GNo
Type Of Funding International - Non Competitive
Category 3IFB
UON Y

Structural analysis and functional inactivation of bacterial transcription complexes$273,000

Funding body: ARC (Australian Research Council)

Funding body ARC (Australian Research Council)
Project Team Professor Peter Lewis, Conjoint Associate Professor Renate Griffith, Dr Richard Lewis
Scheme Discovery Projects
Role Lead
Funding Start 2006
Funding Finish 2006
GNo G0185244
Type Of Funding Aust Competitive - Commonwealth
Category 1CS
UON Y

20055 grants / $274,064

Structural analysis of bacillus subtilis RNA polymerase elongation complexes$25,064

Funding body: ARC (Australian Research Council)

Funding body ARC (Australian Research Council)
Project Team Professor Peter Lewis, Dr Richard Lewis, Assoc. Prof Alasdair McDowall
Scheme Linkage International Award
Role Lead
Funding Start 2005
Funding Finish 2005
GNo G0184314
Type Of Funding Aust Competitive - Commonwealth
Category 1CS
UON Y

2005 RIBG allocation$20,811

Funding body: University of Newcastle

Funding body University of Newcastle
Project Team Professor Peter Lewis
Scheme Research Infrastructure Block Grant (RIBG)
Role Lead
Funding Start 2005
Funding Finish 2005
GNo G0185832
Type Of Funding Internal
Category INTE
UON Y

Structural Analysis and Functional Inactivation of Bacillus Subtilis RNA Polymerase$13,000

Funding body: University of Newcastle

Funding body University of Newcastle
Project Team Professor Peter Lewis, Conjoint Associate Professor Renate Griffith
Scheme Project Grant
Role Lead
Funding Start 2005
Funding Finish 2005
GNo G0184652
Type Of Funding Internal
Category INTE
UON Y

Development of genetically engineered systems to monitor the bioavailability of pollutants in soil and water samples$8,000

Funding body: University of Newcastle

Funding body University of Newcastle
Project Team Professor Peter Lewis
Scheme Project Grant
Role Lead
Funding Start 2005
Funding Finish 2005
GNo G0184651
Type Of Funding Internal
Category INTE
UON Y

20044 grants / $795,450

Multifunctional confocal laser scanning microscope with time resolved and two photon imaging and fluorescence correlation capabilities$579,000

Funding body: Australian Research Council

Funding body Australian Research Council
Project Team
Scheme Linkage Infrastructure Equipment & Facilities (LIEF)
Role Investigator
Funding Start 2004
Funding Finish 2004
GNo
Type Of Funding Aust Competitive - Commonwealth
Category 1CS
UON Y

In vivo studies on the regulation of transcription and translation in Bacillus subtilis$210,000

Funding body: ARC (Australian Research Council)

Funding body ARC (Australian Research Council)
Project Team Professor Peter Lewis
Scheme Discovery Projects
Role Lead
Funding Start 2004
Funding Finish 2004
GNo G0182839
Type Of Funding Aust Competitive - Commonwealth
Category 1CS
UON Y

Investigation of transcript factor interaction with RNA polymerase in Bacillus subtilis$6,000

Funding body: University of Newcastle

Funding body University of Newcastle
Project Team Professor Peter Lewis, Conjoint Associate Professor Renate Griffith
Scheme Project Grant
Role Lead
Funding Start 2004
Funding Finish 2004
GNo G0183378
Type Of Funding Internal
Category INTE
UON Y

ASM 2004 Sydney, 26 Sept - 1 Oct 2004$450

Funding body: University of Newcastle

Funding body University of Newcastle
Project Team Professor Peter Lewis
Scheme Travel Grant
Role Lead
Funding Start 2004
Funding Finish 2004
GNo G0184930
Type Of Funding Internal
Category INTE
UON Y

20032 grants / $10,900

Protein-protein interactions in Bacillus subtilis.$8,500

Funding body: University of Newcastle

Funding body University of Newcastle
Project Team Professor Peter Lewis, Conjoint Associate Professor Renate Griffith
Scheme Project Grant
Role Lead
Funding Start 2003
Funding Finish 2003
GNo G0182487
Type Of Funding Internal
Category INTE
UON Y

International Conference of Functional Genomics of Gram-positive Microorganisms, Baveno, Italy 22-28 June, 2003$2,400

Funding body: University of Newcastle

Funding body University of Newcastle
Project Team Professor Peter Lewis
Scheme Travel Grant
Role Lead
Funding Start 2003
Funding Finish 2003
GNo G0183093
Type Of Funding Internal
Category INTE
UON Y

20023 grants / $17,400

Investigation of Protein-Protein Interactions in Bacillus subtillis$10,000

Funding body: University of Newcastle

Funding body University of Newcastle
Project Team Professor Peter Lewis, Conjoint Associate Professor Renate Griffith
Scheme Project Grant
Role Lead
Funding Start 2002
Funding Finish 2002
GNo G0181282
Type Of Funding Internal
Category INTE
UON Y

Subcellular localisation of translation factors in Bacillus subtillis$5,000

Funding body: University of Newcastle

Funding body University of Newcastle
Project Team Professor Peter Lewis
Scheme Project Grant
Role Lead
Funding Start 2002
Funding Finish 2002
GNo G0181368
Type Of Funding Internal
Category INTE
UON Y

Prokaryotic Development Canada, 10-14 July, 2002$2,400

Funding body: University of Newcastle

Funding body University of Newcastle
Project Team Professor Peter Lewis
Scheme Travel Grant
Role Lead
Funding Start 2002
Funding Finish 2002
GNo G0182003
Type Of Funding Internal
Category INTE
UON Y

20013 grants / $224,156

Characterisation of transcription foci, their role in chromosome segregation, and the localisation of transcription and translation factors in Bacillus subtilis$211,350

Funding body: ARC (Australian Research Council)

Funding body ARC (Australian Research Council)
Project Team Professor Peter Lewis
Scheme Large Grant
Role Lead
Funding Start 2001
Funding Finish 2001
GNo G0179272
Type Of Funding Aust Competitive - Commonwealth
Category 1CS
UON Y

Subcellular localisation of energy generating enzymes in the bacterium Bacillus subtilis.$12,000

Funding body: University of Newcastle

Funding body University of Newcastle
Project Team Professor Peter Lewis
Scheme Project Grant
Role Lead
Funding Start 2001
Funding Finish 2001
GNo G0179953
Type Of Funding Internal
Category INTE
UON Y

ComBio 2001 National Convention Centres, Canberra 1-4 October, 2001$806

Funding body: University of Newcastle

Funding body University of Newcastle
Project Team Professor Peter Lewis
Scheme Travel Grant
Role Lead
Funding Start 2001
Funding Finish 2001
GNo G0181575
Type Of Funding Internal
Category INTE
UON Y

20001 grants / $15,000

High resolution microscopic studies on the internal organisation of the bacterium Bacillus subtilis.$15,000

Funding body: University of Newcastle

Funding body University of Newcastle
Project Team Professor Peter Lewis
Scheme New Staff Grant
Role Lead
Funding Start 2000
Funding Finish 2000
GNo G0179918
Type Of Funding Internal
Category INTE
UON Y
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Research Supervision

Number of supervisions

Completed20
Current5

Total current UON EFTSL

PhD2

Current Supervision

Commenced Level of Study Research Title / Program / Supervisor Type
2015 PhD Elucidating the Function of the Novel Transcription Factor AtfA and its Relevance to Virulence and Persistence in the y Proteobacteria
Biological Sciences, Faculty of Science and Information Technology, The University of Newcastle
Principal Supervisor
2013 PhD Understanding Chromosome Dimer Resolution Systems of a Pathogenic Bacteria at a Molecular Level
Biological Sciences, Faculty of Science and Information Technology, The University of Newcastle
Co-Supervisor
2012 PhD Development of Genetic Tools for the Quantitative Assessment of Gene Depletion in Pathogenic Bacteria
Biological Sciences, Faculty of Science and Information Technology, The University of Newcastle
Co-Supervisor
2012 PhD Bacterial Transcription Factors NusB, NusE ATFA and p as Targets for Antibiotic Research
Biological Sciences, Faculty of Science and Information Technology, The University of Newcastle
Principal Supervisor
2006 PhD Structural analysis of RNA polymerase
Microbiology, University of Newcastle
Sole Supervisor

Past Supervision

Year Level of Study Research Title / Program / Supervisor Type
2014 PhD Increased Understanding of the Molecular Interactions Involved in Bacterial Transcription and Recombination
Biological Sciences, Faculty of Science and Information Technology, The University of Newcastle
Principal Supervisor
2013 PhD Investigation of the Transcription Complex in Acinetobacter baylyi ADP1 and the Identification of the Novel Small Acidic Transcription Factor AtfA
Biological Sciences, Faculty of Science and Information Technology, The University of Newcastle
Principal Supervisor
2013 PhD Genetic Regulation of Embryo Development and Formation of Seed Storage Products in the Legume Model Medicago truncatula
Biological Sciences, Faculty of Science and Information Technology, The University of Newcastle
Co-Supervisor
2012 PhD The Characterisation of the Interaction between PcrA and RNA Polymerase
Biological Sciences, Faculty of Science and Information Technology, The University of Newcastle
Sole Supervisor
2011 PhD The RNA Polymerase-Q Interaction as a Target for Potential Novel Antimicrobials
Biological Sciences, Faculty of Science and Information Technology, The University of Newcastle
Principal Supervisor
2010 PhD The Structure of Bacterial RNA Polymerase in Complex With the Essential Transcription Elongation Factor NusA
Biological Sciences, Faculty of Science and Information Technology, The University of Newcastle
Sole Supervisor
2008 PhD The Composition of Transcription Complexes in Bacillus Subtilis
Biological Sciences, Faculty of Science and Information Technology, The University of Newcastle
Sole Supervisor
2007 Honours Interaction of C-terminus of PcrA with the B subunit of RNA polymerase
Microbiology, University of Newcastle
Sole Supervisor
2006 Honours Characterisation of the interaction between PcrA and the B-subunit of RNA polymerase
Microbiology, University of Newcastle
Sole Supervisor
2005 Honours Development of an engineered Bacillus subtilis strain for the detection of bioavailable environmental toxicants
Microbiology, University of Newcastle
Sole Supervisor
2005 Honours Characterisation of the interaction between PcrA and the beta subunit of RNA polymerase
Microbiology, University of Newcastle
Sole Supervisor
2005 Honours Interaction of transcription factors NusB and NusE in Bacillus subtilis
Microbiology, University of Newcastle
Sole Supervisor
2005 Honours The design and testing of inhibitors of interactions between essential transcription factors and RNA polymerase
Microbiology, University of Newcastle
Sole Supervisor
2003 Honours Interactions between RNA polymerase and transcription factors: An integrated approach
Microbiology, University of Newcastle
Sole Supervisor
2003 Honours Subcellular localisation of the transcription elongation factors NusB, NusG and GreA in Bacillus subtilis
Microbiology, University of Newcastle
Sole Supervisor
2003 Honours Analysis of translation elongation factors EF-Tu and EF-G in Bacillus subtilis
Microbiology, University of Newcastle
Sole Supervisor
2003 Honours Analysis of translation initiation factors in Bacillus subtilis
Microbiology, University of Newcastle
Sole Supervisor
2002 Honours Subcellular localisation of tranlsation factors in Bacillus subtilis
Microbiology, University of Newcastle
Sole Supervisor
2001 Honours Subcellular localisation of ATP synthase in Bacillus subtilis
Microbiology, University of Newcastle
Sole Supervisor
2001 Honours Subcellular localisation of NusA in Bacillus subtilis
Microbiology, University of Newcastle
Sole Supervisor
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Professor Peter Lewis

Position

Professor
School of Environmental and Life Sciences
Faculty of Science and Information Technology

Focus area

Biological Sciences

Contact Details

Email peter.lewis@newcastle.edu.au
Phone (02) 4921 5701
Fax (02) 4921 5472

Office

Room BG10
Building Biological Sciences
Location Callaghan
University Drive
Callaghan, NSW 2308
Australia
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