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Career Summary

Biography

My research career has focused on the gram positive bacterium Bacillus subtilis. During my PhD I purified and characterised the replication terminator protein (RTP) and its DNA binding sites (Lewis et al., J. Bacteriol 171, 3564-3567 (1989); Lewis et al., J. Mol. Biol. 214, 73-84 (1990)). These sites are regions of DNA where oppositely moving replication forks meet and newly replicated chromosomes are resolved prior to segregation. During my post-doctoral research I was responsible for the development of cell biological techniques and vectors for visualising gene expression and protein localisation in live bacterial cells (Lewis et al., PNAS 91, 3849-3853 (1994); Lewis et al., Mol Microbiol 13, 655-662 (1994); Lewis and Errington, Microbiology 142, 733-740 (1996); Lewis and Marston, Gene 227, 101-109 (1999); Feucht and Lewis, Gene 264, 289-297 (2001)). These techniques have been widely adopted by research groups involved in microbial cell biology, and the plasmid vectors have now been distributed directly to well over 100 laboratories world-wide, although they are now also distributed by the Bacillus Genetic Stock Centre. These techniques were instrumental in determining the establishment of compartment specificity of developmentally regulated s-factors during sporulation in B. subtilis (Lewis et al., PNAS 91, 3849-3853 (1994)). I also showed that prespore specific accumulation of the transcription regulator SpoIIAA was responsible for initiation of compartment-specific gene expression during development, that this accumulation was probably due to prespore-specific activity of the phosphatase SpoIIE, and that a programme of proteolysis was initiated following this activation event (Lewis et al., Genes Cell 1, 881-894 (1996); Lewis et al., J. Bacteriol 180, 3276-3284 (1998)). I was also involved in work that showed that the highly conserved SpoIIIE (FtsK) protein is a DNA translocase that moves DNA through a division septum (Wu et al., Genes Dev 9, 1316-1326 (1995)). This was the first example of such a phenomenon, and was a very significant finding as previously it was assumed that DNA was segregated into daughter cells/different compartments prior to division septum formation. Finally, I have shown that transcription and translation are spatially separated within bacteria (Lewis et al., EMBO J 19, 710-718 (2000)). This was an unexpected result as the 2 processes were thought to be very tightly coupled in bacteria. Furthermore, transcription becomes concentrated into a sub-fraction of the bacterial nucleoid at higher growth rates. These transcription foci have been shown to be the sites of rRNA synthesis, and my laboratory is now focussing on characterising transcription complexes and their structure in detail.

Qualifications

  • Doctor of Philosophy, University of Sydney, 14/06/1991
  • Bachelor of Science, University of Sheffield - UK, 19/07/1986

Research

Research keywords

  • Microscopy
  • Molecular Microbiology
  • Transcription regulation

Research expertise

My research career has focused on the gram positive bacterium Bacillus subtilis. During my PhD I purified and characterised the replication terminator protein (RTP) and its DNA binding sites (Lewis et al., J. Bacteriol 171, 3564-3567 (1989); Lewis et al., J. Mol. Biol. 214, 73-84 (1990)). These sites are regions of DNA where oppositely moving replication forks meet and newly replicated chromosomes are resolved prior to segregation. During my post-doctoral research I was responsible for the development of cell biological techniques and vectors for visualising gene expression and protein localisation in live bacterial cells (Lewis et al., PNAS 91, 3849-3853 (1994); Lewis et al., Mol Microbiol 13, 655-662 (1994); Lewis and Errington, Microbiology 142, 733-740 (1996); Lewis and Marston, Gene 227, 101-109 (1999); Feucht and Lewis, Gene 264, 289-297 (2001)). These techniques have been widely adopted by research groups involved in microbial cell biology, and the plasmid vectors have now been distributed directly to well over 100 laboratories world-wide, although they are now also distributed by the Bacillus Genetic Stock Centre. These techniques were instrumental in determining the establishment of compartment specificity of developmentally regulated s-factors during sporulation in B. subtilis (Lewis et al., PNAS 91, 3849-3853 (1994)). I also showed that prespore specific accumulation of the transcription regulator SpoIIAA was responsible for initiation of compartment-specific gene expression during development, that this accumulation was probably due to prespore-specific activity of the phosphatase SpoIIE, and that a programme of proteolysis was initiated following this activation event (Lewis et al., Genes Cell 1, 881-894 (1996); Lewis et al., J. Bacteriol 180, 3276-3284 (1998)). I was also involved in work that showed that the highly conserved SpoIIIE (FtsK) protein is a DNA translocase that moves DNA through a division septum (Wu et al., Genes Dev 9, 1316-1326 (1995)). This was the first example of such a phenomenon, and was a very significant finding as previously it was assumed that DNA was segregated into daughter cells/different compartments prior to division septum formation. Finally, I have shown that transcription and translation are spatially separated within bacteria (Lewis et al., EMBO J 19, 710-718 (2000)). This was an unexpected result as the 2 processes were thought to be very tightly coupled in bacteria. Furthermore, transcription becomes concentrated into a sub-fraction of the bacterial nucleoid at higher growth rates. These transcription foci have been shown to be the sites of rRNA synthesis, and my laboratory is now focussing on characterising transcription complexes and their structure in detail.

Fields of Research

CodeDescriptionPercentage
060199Biochemistry And Cell Biology Not Elsewhere Classified50
110800Medical Microbiology35
060499Genetics Not Elsewhere Classified15

Memberships

ARC Committee member

  • OzReader - Biological Sciences

Body relevant to professional practice.

  • Member of EU 6th Framework Consortium for A$20M EU 6th framework BaSysBio project - European Union (EU) Framework Collaboration

Appointments

National Convener Molecular Microbiology Special Interest Group
Australian Society for Microbiology (Australia)
01/07/2005

Awards

Research Award.

2004International Short-Term Fellowship
Australian Academy of Sciences (Australia)
Provided funds for short term fellowship in lab of Dr Philippe Noirot, Jouy-en-Josas, France to perform a genomic yeast 2-hybrid screen of proteins that interact with RNA polymerase. This fellowship laid the foundation for the subsequent BaSysBio EU 6th framework application and placed me as a central figure in the establishment of the consortium.

Invitations

Invited speaker
Conference, United Kingdom (Conference Presentation - non published.)
2007
Reviewer
Various Publishers in the field of Environmental and Life Science, Australia (Invitation to review submitted papers to top academic journals in the field)
2007

Administrative

Administrative expertise

No expertise.

Teaching

Teaching keywords

  • Molecular Biology

Teaching expertise

Molecular biology, particularly bacterial. Microbiology.

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Highlighted Publications

For publications that are currently unpublished or in-press, details are shown in italics.

YearCitationAltmetricsLink
2006Doherty GP, Meredith DH, Lewis PJ, 'Subcellular partitioning of transcription factors in Bacillus subtilis', Journal of Bacteriology, 188 4101-4110 (2006) [C1]
2005Davies KM, Dedman AJ, Van Horck SH, Lewis PJ, 'The NusA : RNA polymerase ratio is increased at sites of rRNA synthesis in Bacillus subtilis', Molecular Microbiology, 57 366-379 (2005) [C1]
2004Lewis PJ, 'Bacterial subcellular architecture: recent advances and future prospects', Molecular Microbiology, 54 1135-1150 (2004) [C1]
2004Migocki MD, Lewis PJ, Wake RG, Harry EJ, 'The midcell replication factory in Bacillus subtilis is highly mobile: implications for coordinating chromosome replication with other cell cycle events', Molecular Microbiology, 54 452-463 (2004) [C1]

Publications

For publications that are currently unpublished or in-press, details are shown in italics.

Click on a category title below to expand the list of citations for that specific category.

Chapter (3 outputs)

YearCitationAltmetricsLink
2012Doherty GP, Mettrick KA, Grainge IR, Lewis PJ, 'Imaging fluorescent protein fusions in live bacteria', Methods in Microbiology, Academic Press, Kidlington, Ox 107-126 (2012) [B1]

Co-authors: Ian Grainge

2008Lewis PJ, 'Subcellular organisation in bacteria', Bacterial Physiology: A Molecular Approach, Springer, Berlin 1-42 (2008) [B1]
2007Lewis PJ, 'The organization of transcription and translation', Bacillus: Cellular and Molecular Biology, Caister Academic Press, Norfolk, United Kingdom 135-166 (2007) [B1]

Journal article (40 outputs)

YearCitationAltmetricsLink
2014Keller AN, Yang X, Wiedermannová J, Delumeau O, Krásný L, Lewis PJ, 'e, a new subunit of RNA polymerase found in gram-positive bacteria.', J Bacteriol, 196 3622-3632 (2014)
2014Bi Y, Ma C, Zhang H, Zhou Z, Yang J, Zhang Z, et al., 'Novel 3-substituted ocotillol-type triterpenoid derivatives as antibacterial candidates', Chemical Biology and Drug Design, (2014)
2014Kandemir H, Kutty SK, Black DS, Kumar N, Ma C, Lewis PJ, Griffith R, 'Synthesis and biological evaluation of 2,5-di(7-indolyl)-1,3,4-oxadiazoles, and 2- and 7-indolyl 2-(1,3,4-thiadiazolyl)ketones as antimicrobials', Bioorganic and Medicinal Chemistry, (2014)
2014Withers R, Doherty GP, Jordan M, Yang X, Dixon NE, Lewis PJ, 'AtfA, a new factor in global regulation of transcription in Acinetobacter spp', Molecular Microbiology, 93 1130-1143 (2014)
2014Withers R, Doherty GP, Jordan M, Yang X, Dixon NE, Lewis PJ, 'AtfA, a new factor in global regulation of transcription in Acinetobacter spp.', Mol Microbiol, 93 1130-1143 (2014)
2014Mielczarek M, Devakaram RV, Ma C, Yang X, Kandemir H, Purwono B, et al., 'Synthesis and biological activity of novel bis-indole inhibitors of bacterial transcription initiation complex formation', Organic and Biomolecular Chemistry, 12 2882-2894 (2014)
2013Brzoska AJ, Hassan KA, de Leon EJ, Paulsen IT, Lewis PJ, 'Single-Step Selection of Drug Resistant Acinetobacter baylyi ADP1 Mutants Reveals a Functional Redundancy in the Recruitment of Multidrug Efflux Systems', PLOS ONE, 8 (2013) [C1]
2013Zhou Z, Ma C, Zhang H, Bi Y, Chen X, Tian H, et al., 'Synthesis and biological evaluation of novel ocotillol-type triterpenoid derivatives as antibacterial agents', European Journal of Medicinal Chemistry, 68 444-453 (2013) [C1]
2013Ma C, Yang X, Kandemir H, Mielczarek M, Johnston EB, Griffith R, et al., 'Inhibitors of Bacterial Transcription Initiation Complex Formation', ACS Chemical Biology, 8 1972-1980 (2013) [C1]
2012Buescher JM, Liebermeister W, Jules M, Uhr M, Muntel J, Botella E, et al., 'Global network reorganization during dynamic adaptations of Bacillus subtilis metabolism', Science, 335 1099-1103 (2012) [C1]
2012Buncherd H, Nessen MA, Nouse N, Stelder SK, Roseboom W, Dekker HL, et al., 'Selective enrichment and identification of cross-linked peptides to study 3-D structures of protein complexes by mass spectrometry', Journal of Proteomics, 75 2205-2215 (2012) [C1]
2012Nicolas P, Mader U, Dervyn E, Rochat T, Leduc A, Pigeonneau N, et al., 'Condition-dependent transcriptome reveals high-level regulatory architecture in Bacillus subtilis', Science, 335 1103-1106 (2012) [C1]
2011Liew ATF, Theis T, Jensen SO, Garcia-Lara J, Foster SJ, Firth N, et al., 'A simple plasmid-based system that allows rapid generation of tightly controlled gene expression in Staphylococcus aureus', Microbiology, 157 666-676 (2011) [C1]
2010Doherty GP, Bailey KL, Lewis PJ, 'Stage-specific fluorescence intensity of GFP and mCherry during sporulation in Bacillus Subtilis', BMC Research Notes, 3 1-8 (2010) [C1]
2010Doherty GP, Fogg MJ, Wilkinson AJ, Lewis PJ, 'Small subunits of RNA polymerase: Localization, levels and implications for core enzyme composition', Microbiology, 16 3532-3543 (2010) [C1]
2010Robinson A, Brzoska AJ, Turner KM, Withers RW, Harry EJ, Lewis PJ, Dixon NE, 'Essential biological processes of an emerging pathogen: DNA replication, transcription, and cell division in Acinetobacter spp', Microbiology and Molecular Biology Reviews, 74 273-297 (2010) [C1]
2010Yang X, Lewis PJ, 'The interaction between RNA polymerase and the elongation factor NusA', RNA Biology, 7 272-275 (2010) [C1]
2010Botella E, Fogg M, Jules M, Piersma S, Doherty GP, Hansen A, et al., 'pBaSysBioII: An integrative plasmid generating gfp transcriptional fusions for high-throughput analysis of gene expression in Bacillus subtilis', Microbiology, 156 1600-1608 (2010) [C1]
2010Yang X, Lewis PJ, 'The interaction between bacterail transcription factors and RNA polymerase during the transition from initiation to elongation', Transcription, 1 1-4 (2010) [C1]
2009Johnston EB, Lewis PJ, Griffith R, 'The interaction of Bacillus subtilis sigma(A) with RNA polymerase', Protein Science, 18 2287-2297 (2009) [C1]
2009Yang X, Molimau S, Doherty GP, Johnston EB, Marles-Wright J, Rothnagel R, et al., 'The structure of bacterial RNA polymerase in complex with the essential transcription elongation factor NusA', EMBO Reports, 10 997-1002 (2009) [C1]
2008Yang X, Lewis PJ, 'Overproduction and purification of recombinant Bacillus subtilis RNA polymerase', Protein Expression and Purification, 59 86-93 (2008) [C1]
2008Yang X, Doherty GP, Lewis PJ, 'Tandem affinity purification vectors for use in gram positive bacteria', Plasmid, 59 54-62 (2008) [C1]
2008Huo N, Lewis PJ, 'Green fluorescent protein labeling of ylyA gene in Bacillus subtilis', Weishengwu Xuebao, 48 829-833 (2008) [C1]
2008Meredith DH, Plank MW, Lewis PJ, 'Different patterns of integral membrane protein localization during cell division in Bacillus subtilis', Microbiology, 154 64-71 (2008) [C1]
2008Lewis PJ, Doherty GP, Clarke J, 'Transcription factor dynamics', Microbiology, 154 1837-1844 (2008) [C1]
2008Marles-Wright J, Grant T, Delumeau O, Van Duinen G, Firbank SJ, Lewis PJ, et al., 'Molecular architecture of the 'stressosome,' a signal integration and transduction hub', Science, 322 92-96 (2008) [C1]
2006Doherty GP, Meredith DH, Lewis PJ, 'Subcellular partitioning of transcription factors in Bacillus subtilis', Journal of Bacteriology, 188 4101-4110 (2006) [C1]
2005Davies KM, Dedman AJ, Van Horck SH, Lewis PJ, 'The NusA : RNA polymerase ratio is increased at sites of rRNA synthesis in Bacillus subtilis', Molecular Microbiology, 57 366-379 (2005) [C1]
2005MacDougall IJA, Lewis PJ, Griffith R, 'Homology modelling of RNA polymerase and associated transcription factors from Bacillus subtilis', Journal of Molecular Graphics & Modelling, 23 297-303 (2005) [C1]
2005Davies KM, Dedman AJ, van Horck S, Lewis PJ, 'The NusA:RNA polymerase ratio is increased at sites of rRNA synthesis in Bacillus subtilis.', Mol Microbiol, 57 366-379 (2005)
2004Migocki MD, Lewis PJ, Wake RG, Harry EJ, 'The midcell replication factory in Bacillus subtilis is highly mobile: implications for coordinating chromosome replication with other cell cycle events', Molecular Microbiology, 54 452-463 (2004) [C1]
2004Lewis PJ, 'Bacterial subcellular architecture: recent advances and future prospects', Molecular Microbiology, 54 1135-1150 (2004) [C1]
2004Johnson AS, Van Horck SH, Lewis PJ, 'Dynamic localization of membrance proteins in Bacillus subtilis', Microbiology, 150 2815-2824 (2004) [C1]
2003Davies KM, Lewis PJ, 'Localization of rRNA Synthesis in Bacillus subtilis: Characterization of Loci Involved in Transcription Focus Formation', Journal of Bacteriology, 185 2346-2353 (2003) [C1]
2003Harry EJ, Lewis PJ, 'Early targeting of Min proteins to the cell poles in germinated spores of Bacillus subtilis: evidence for division apparatus-independent recruitment of Min proteins to the division site', Molecular Microbiology, 47 37-48 (2003) [C1]
2001Feucht A, Lewis PJ, 'Improved plasmid vectors for the production of multiple fluorescent protein fusions in Bacillus subtilis', Gene, 264 289-297 (2001) [C1]
2001Lewis PJ, 'Bacterial chromosome segregation', Microbiology, 147 519-526 (2001) [C1]
2000Meijer WJJ, Lewis PJ, Errington J, Salas M, 'Dynamic relocalization of phage f29 DNA during replication and the role of the viral protein p16.7', EMBO Journal, 19 4182-4190 (2000)
1994LEWIS PJ, NWOGUH CE, BARER MR, HARWOOD CR, ERRINGTON J, 'USE OF DIGITIZED VIDEO MICROSCOPY WITH A FLUOROGENIC ENZYME-SUBSTRATE TO DEMONSTRATE CELL-SPECIFIC AND COMPARTMENT-SPECIFIC GENE-EXPRESSION IN SALMONELLA-ENTERITIDIS AND BACILLUS-SUBTILIS', MOLECULAR MICROBIOLOGY, 13 655-662 (1994)
Show 37 more

Conference (21 outputs)

YearCitationAltmetricsLink
2008Lewis PJ, 'Subcellular localization of transcription factors', Society for General Microbiology Spring Meeting Abstracts, Edinburgh, Scotland (2008) [E3]
2008Robinson A, Brzoska AJ, Turner K, Harry L, Lewis PJ, Dixon N, 'DNA replication in pathogenic bacteria: Understanding and disrupting protein-protein interactions', ASM 2008 Melbourne: Program and Abstracts Book, Melbourne, VIC (2008) [E3]
2008Brzoska AJ, Lewis PJ, 'Identification of novel protein-protein interactions in S. aureus', International Symposium on Staphylococci and Staphylococcal Infections: Abstracts, Cairns, QLD (2008) [E3]
2008Withers RW, Brzoska AJ, Lewis PJ, 'Establishment of an in vitro transcription assay for Acinetobacter spp', ASM 2008 Melbourne: Program and Abstracts Book, Melbourne, VIC (2008) [E3]
2008Turner K, Brzoska AJ, Robinson A, Firth N, Lewis PJ, Dixon N, Harry L, 'Isolation of novel cell division complexes using tandem affinity purification', ASM 2008 Melbourne: Program and Abstracts Book, Melbourne, VIC (2008) [E3]
2008Brzoska AJ, Turner K, Robinson A, Dixon N, Harry L, Lewis PJ, 'Identification of novel protein-protein interactions in Acinetobacter spp', ASM 2008 Melbourne: Program and Abstracts Book, Melbourne, VIC (2008) [E3]
2007Harriott K, Lewis PJ, 'Characterisation of the interaction between PcrA and the b sub-unit of RNA polymerase', BacPath 9 : The Molecular Biology of Bacterial Pathogens. Abstracts, Lorne, VIC (2007) [E3]
2007Lewis PJ, Doherty GP, Meredith DH, 'Transcription factor dynamics', ASM 2007 Adelaide Program and Abstracts Book, Adelaide, SA (2007) [E3]
2007Johnston EB, Griffith R, Lewis PJ, 'The design and testing of inhibitors of interactions between essential transcription factors and RNA polymerase', BacPath 9 : The Molecular Biology of Bacterial Pathogens. Abstracts, Lorne, VIC (2007) [E3]
2007Johnston EB, Griffith R, Lewis PJ, 'The design and testing of inhibitors of interactions between essential transcription factors and RNA polymerase', 32nd Lorne Conference on Protein Structure and Function. Abstracts, Lorne, Vic (2007) [E3]
2007Yang X, Lewis PJ, 'Structure of transcription factor NUSA-RNA polymerase complex', ASM 2007 Adelaide Program and Abstracts Book, Adelaide, SA (2007) [E3]
2007Lewis PJ, Meredith DH, Plank MW, 'Selective removal of integral membrane proteins from the mid-cell site at the onset of cell division', 4th Conference on Functional Genomics of Gram-Positive Microorganisms. Abstracts, Tirrenia, Pisa, Italy (2007) [E3]
2004Migocki MD, Lewis PJ, Wake G, Harry E, 'Is chromosome replication involved in the spatial regulation of midcell division in Bacillus subtilis?', ASM 2004 Sydney National Conference, Sydney, Australia (2004) [E3]
2004MacDougall IJA, Lewis PJ, Griffith R, 'Molecular modelling of the RNA polymerase complex in Bacillus subtilis', MM2004: 9th Molecular Modelling Workshop, Sydney, Australia (2004) [E3]
2004Lewis PJ, 'Subcellular organization of transcription in bacteria', ASM 2004 Sydney National Conference, Sydney, Australia (2004) [E3]
2004Martin S, MacDougall IJA, Griffith R, Lewis PJ, 'Investigation of the interactions between transcription factor NusA with RNA polymerase in Bacillus subtilis', ASM 2004 Sydney National Conference, Sydney, Australia (2004) [E3]
2004Doherty GP, Meredith DH, Lewis PJ, 'Composition of Transcription complexes in Bacillus subtilis', ASM 2004 Sydney National Conference, Sydney, Australia (2004) [E3]
2003Davies KM, Dedman A, Walsh AK, Van Horck SH, Lewis PJ, 'The bacterial transcription factor NusA is preferentially recruited to sites of rRNA synthesis: Direct evidence for differential RNAP elongation complex formation in vivo', Functional Genomics of Gram-positive Microorganisms 12th International Conference on Bacilli, Baveno, Italy (2003) [E3]
2002Lewis PJ, Harry E, 'Cellular targeting of proteins involved in positioning the division site in bacteria', Proceedings of the Australian Society for Biochemistry and Molecular Biology, Sydney, NSW (2002) [E3]
2002Lewis PJ, Davies KM, 'Analysis of transcription foci in bacillus subtilis', American Society for Microbiology - ASM Conference: Prokaryotic Development, Quebec, CANADA (2002) [E3]
2001Lewis PJ, 'Compartmentalisation of transcription and translation in bacteria', Australian Society for Biochemistry & Molecular Biology Incorp - Combio 2001, Canberra ACT (2001) [E3]
Show 18 more
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Grants and Funding

Summary

Number of grants45
Total funding$26,063,564

- Indicates that the researcher may be seeking students for this project.

Click on a grant title below to expand the full details for that specific grant.

2014 (1 grants)

Development of compounds that inhibit transcription initiation $20,000
Funding Body: University of Newcastle

Project Team
Professor Peter Lewis
SchemeRole
Near Miss GrantChief Investigator
Total AmountFunding StartFunding Finish
$20,00020142014
GNo:G1301396

2013 (3 grants)

Characterisation of a macromolecular complex essential for rapid microbial cell proliferation$20,000
Funding Body: Hunter Medical Research Institute

Project Team
Professor Peter Lewis
SchemeRole
Near MissChief Investigator
Total AmountFunding StartFunding Finish
$20,00020132013
GNo:G1300698

Characterisation of a macromolecular complex essential for rapid microbial cell proliferation$20,000
Funding Body: University of Newcastle

Project Team
Professor Peter Lewis, Doctor Xiao Yang
SchemeRole
Near Miss GrantChief Investigator
Total AmountFunding StartFunding Finish
$20,00020132013
GNo:G1300482

New Drugs to Kill Superbugs$13,000
Funding Body: University of Newcastle - Faculty of Science & IT

Project Team
Professor Peter Lewis, Professor Adam McCluskey, Doctor Chris Gordon, Doctor Joey Ambrus
SchemeRole
Strategic Small GrantChief Investigator
Total AmountFunding StartFunding Finish
$13,00020132013
GNo:G1401065

2012 (3 grants)

Targeting nucleic acid synthesis and cell division in gram-negative bacterial pathogens$311,439
Funding Body: NHMRC (National Health & Medical Research Council)

Project Team
Professor Nicholas Dixon, Associate Professor Elizabeth Harry, Professor Peter Lewis, Associate Professor Aaron Oakley, Doctor Ian Grainge
SchemeRole
Project GrantChief Investigator
Total AmountFunding StartFunding Finish
$311,43920122014
GNo:G1101133

Ultrasonic Homogenizer System and -80 ºC Freezers for chemical and biological sample storage$10,000
Funding Body: NHMRC (National Health & Medical Research Council)

Project Team
Professor Adam McCluskey, Professor Eileen McLaughlin, Professor Peter Lewis, Ms Belinda Nixon, Doctor Shaun Roman, Doctor Jennette Sakoff, Doctor Ian Grainge
SchemeRole
Equipment GrantInvestigator
Total AmountFunding StartFunding Finish
$10,00020122012
GNo:G1100986

Faculty Visiting Fellowship 2012$1,987
Funding Body: University of Newcastle - Faculty of Science & IT

Project Team
Professor Peter Lewis
SchemeRole
Visiting FellowshipChief Investigator
Total AmountFunding StartFunding Finish
$1,98720122012
GNo:G1401126

2011 (6 grants)

Exploitation of bacterial transcription initiation as a target for new antimicrobials$521,706
Funding Body: NHMRC (National Health & Medical Research Council)

Project Team
Professor Peter Lewis, Associate Professor Renate Griffith, Associate Professor Naresh Kumar
SchemeRole
Project GrantChief Investigator
Total AmountFunding StartFunding Finish
$521,70620112013
GNo:G1000285

Establishment of a comprehensive regional biophysical analysis facility $500,000
Funding Body: ARC (Australian Research Council)

Project Team
Professor Peter Lewis, Professor Nicholas Dixon, Professor Eileen McLaughlin, Professor Adam McCluskey, Associate Professor Aaron Oakley, Professor Paul Foster, Laureate Professor John Aitken, Associate Professor Jennifer Beck, Dr Michael Kelso, Professor Graham Nicholson, Associate Professor Elizabeth Harry, Professor Ian Charles, Associate Professor Steven Djordjevic
SchemeRole
Linkage Infrastructure Equipment & Facilities (LIEF)Chief Investigator
Total AmountFunding StartFunding Finish
$500,00020112011
GNo:G1000630

Chemical Biology$444,961
Funding Body: University of Newcastle

Structure function analysis of the NusA-RNA polymerase interaction$300,000
Funding Body: ARC (Australian Research Council)

Project Team
Professor Peter Lewis, Doctor Xiao Yang, Dr Richard Lewis, Dr Jon Marles-Wright
SchemeRole
Discovery ProjectsChief Investigator
Total AmountFunding StartFunding Finish
$300,00020112013
GNo:G1000129

Establishment of a comprehensive regional biophysical analysis facility $230,000
Funding Body: ARC (Australian Research Council)

Project Team
Professor Peter Lewis, Professor Nicholas Dixon, Professor Eileen McLaughlin, Professor Adam McCluskey, Associate Professor Aaron Oakley, Professor Paul Foster, Laureate Professor John Aitken, Associate Professor Jennifer Beck, Dr Michael Kelso, Professor Graham Nicholson, Associate Professor Elizabeth Harry, Professor Ian Charles, Associate Professor Steven Djordjevic
SchemeRole
Linkage Infrastructure Equipment & Facilities (LIEF) Partner fundingChief Investigator
Total AmountFunding StartFunding Finish
$230,00020112011
GNo:G1100484

Eppendorf mastercycler pro with thermomixer comfort and 5430R centrifuge$15,000
Funding Body: NHMRC (National Health & Medical Research Council)

Project Team
Professor Eileen McLaughlin, Professor Peter Lewis, Professor Adam McCluskey, Conjoint Professor Keith Jones, Associate Professor Brett Nixon, Doctor Shaun Roman, Doctor Jennette Sakoff, Doctor Ian Grainge, Doctor Janet Holt, Doctor Xiao Yang
SchemeRole
Equipment GrantInvestigator
Total AmountFunding StartFunding Finish
$15,00020112011
GNo:G1100028

2009 (3 grants)

An Advanced Mass Spectrometry Facility for Applications in Proteomics and Organic Chemistry$495,000
Funding Body: ARC (Australian Research Council)

An Advanced Mass Spectrometry Facility for Applications in Proteomics and Organic Chemistry$50,000
Funding Body: ARC (Australian Research Council)

LAS-4000 Chemiluminescence imaging system$25,000
Funding Body: NHMRC (National Health & Medical Research Council)

Project Team
Professor Eileen McLaughlin, Laureate Professor John Aitken, Professor Peter Lewis, Associate Professor Brett Nixon, Doctor Shaun Roman
SchemeRole
Equipment GrantInvestigator
Total AmountFunding StartFunding Finish
$25,00020092009
GNo:G0189852

2008 (2 grants)

X-ray crystallography resource for membrane proteins and large macromolecular complexes$93,000
Funding Body: ARC (Australian Research Council)

Project Team
Professor Peter Lewis, Doctor Robyn Malby
SchemeRole
Linkage Infrastructure Equipment & Facilities (LIEF)Chief Investigator
Total AmountFunding StartFunding Finish
$93,00020082008
GNo:G0188894

Structural Analysis of Bacterial Transcription Proteins by X-ray Crystallography$14,600
Funding Body: University of Newcastle

Project Team
Doctor Robyn Malby, Professor Peter Lewis
SchemeRole
Pilot GrantInvestigator
Total AmountFunding StartFunding Finish
$14,60020082008
GNo:G0189075

2007 (7 grants)

Nucleic Acid Synthesis and Cell Division in Model Pathogenic Bacteria$740,250
Funding Body: NHMRC (National Health & Medical Research Council)

Project Team
Professor Peter Lewis, Professor Nicholas Dixon, Associate Professor Elizabeth Harry
SchemeRole
Project GrantChief Investigator
Total AmountFunding StartFunding Finish
$740,25020072009
GNo:G0186390

Towards an understanding of dynamic genetic regulation at global scale in bacteria: A systems biology approach$486,000
Funding Body: NHMRC (National Health & Medical Research Council)

Project Team
Professor Peter Lewis
SchemeRole
NHMRC ‐ European Union Collaborative Research GrantsChief Investigator
Total AmountFunding StartFunding Finish
$486,00020072009
GNo:G0186507

Towards an understanding of dynamic transcriptional regulation at a global scale in bacteria: a system biology approach$110,250
Funding Body: Department of Innovation, Industry, Science and Research

Project Team
Professor Peter Lewis
SchemeRole
International Science Linkages (ISL) ProgramChief Investigator
Total AmountFunding StartFunding Finish
$110,25020072009
GNo:G0187015

RNA POLYMERASE PROTEIN INTERACTIONS AND THE REGULATION OF TRANSCRIPTION ELONGATION$16,900
Funding Body: University of Newcastle

Project Team
Professor Peter Lewis
SchemeRole
Near Miss GrantChief Investigator
Total AmountFunding StartFunding Finish
$16,90020072007
GNo:G0187186

Hamamatsu ORCA-AG cooled CCD camera$12,000
Funding Body: NHMRC (National Health & Medical Research Council)

Project Team
Professor Peter Lewis
SchemeRole
Equipment GrantChief Investigator
Total AmountFunding StartFunding Finish
$12,00020072007
GNo:G0188190

4th Conference on Functional Genomics of Gram-Positive Microorganisms, Green Park Resort, Tirrenia, Italy, 24/6/2007 - 28/6/2007$2,500
Funding Body: University of Newcastle

Project Team
Professor Peter Lewis
SchemeRole
Travel GrantChief Investigator
Total AmountFunding StartFunding Finish
$2,50020072007
GNo:G0187783

Towards an understanding of dynamic transcriptional regulation at global scale in bacteria: a systems biology approach. BaSysBio$1
Funding Body: European Commission, European Union

Project Team
Professor Peter Lewis
SchemeRole
Sixth Framework ProgrammeChief Investigator
Total AmountFunding StartFunding Finish
$120072007
GNo:G0186876

2006 (2 grants)

Towards and understanding of dynamic genetic regulation at a global scale in bacteria: a Systems biology approach$20,000,000
Funding Body: European Commission, European Union

SchemeRole
6th FrameworkInvestigator
Total AmountFunding StartFunding Finish
$20,000,00020062007
GNo:17800412

Structural analysis and functional inactivation of bacterial transcription complexes$273,000
Funding Body: ARC (Australian Research Council)

Project Team
Professor Peter Lewis, Conjoint Associate Professor Renate Griffith, Dr Richard Lewis
SchemeRole
Discovery ProjectsChief Investigator
Total AmountFunding StartFunding Finish
$273,00020062008
GNo:G0185244

2005 (5 grants)

High throughput proteomics - Thermo Finnigan ProteomeX LCQ Integrated Proteomicis Workstation$207,189
Funding Body: ARC (Australian Research Council)

Structural analysis of bacillus subtilis RNA polymerase elongation complexes$25,064
Funding Body: ARC (Australian Research Council)

Project Team
Professor Peter Lewis, Dr Richard Lewis, Assoc. Prof Alasdair McDowall
SchemeRole
Linkage International AwardChief Investigator
Total AmountFunding StartFunding Finish
$25,06420052007
GNo:G0184314

2005 RIBG allocation$20,811
Funding Body: University of Newcastle

Project Team
Professor Peter Lewis
SchemeRole
Research Infrastructure Block Grant (RIBG)Chief Investigator
Total AmountFunding StartFunding Finish
$20,81120052005
GNo:G0185832

Structural Analysis and Functional Inactivation of Bacillus Subtilis RNA Polymerase$13,000
Funding Body: University of Newcastle

Project Team
Professor Peter Lewis, Conjoint Associate Professor Renate Griffith
SchemeRole
Project GrantChief Investigator
Total AmountFunding StartFunding Finish
$13,00020052005
GNo:G0184652

Development of genetically engineered systems to monitor the bioavailability of pollutants in soil and water samples$8,000
Funding Body: University of Newcastle

Project Team
Professor Peter Lewis
SchemeRole
Project GrantChief Investigator
Total AmountFunding StartFunding Finish
$8,00020052005
GNo:G0184651

2004 (4 grants)

Multifunctional confocal laser scanning microscope with time resolved and two photon imaging and fluorescence correlation capabilities$579,000
Funding Body: Australian Research Council

SchemeRole
Linkage Infrastructure Equipment & Facilities (LIEF)Investigator
Total AmountFunding StartFunding Finish
$579,00020042004
GNo:17800237

In vivo studies on the regulation of transcription and translation in Bacillus subtilis$210,000
Funding Body: ARC (Australian Research Council)

Project Team
Professor Peter Lewis
SchemeRole
Discovery ProjectsChief Investigator
Total AmountFunding StartFunding Finish
$210,00020042006
GNo:G0182839

Investigation of transcript factor interaction with RNA polymerase in Bacillus subtilis$6,000
Funding Body: University of Newcastle

Project Team
Professor Peter Lewis, Conjoint Associate Professor Renate Griffith
SchemeRole
Project GrantChief Investigator
Total AmountFunding StartFunding Finish
$6,00020042004
GNo:G0183378

ASM 2004 Sydney, 26 Sept - 1 Oct 2004$450
Funding Body: University of Newcastle

Project Team
Professor Peter Lewis
SchemeRole
Travel GrantChief Investigator
Total AmountFunding StartFunding Finish
$45020042004
GNo:G0184930

2003 (2 grants)

Protein-protein interactions in Bacillus subtilis.$8,500
Funding Body: University of Newcastle

Project Team
Professor Peter Lewis, Conjoint Associate Professor Renate Griffith
SchemeRole
Project GrantChief Investigator
Total AmountFunding StartFunding Finish
$8,50020032003
GNo:G0182487

International Conference of Functional Genomics of Gram-positive Microorganisms, Baveno, Italy 22-28 June, 2003$2,400
Funding Body: University of Newcastle

Project Team
Professor Peter Lewis
SchemeRole
Travel GrantChief Investigator
Total AmountFunding StartFunding Finish
$2,40020032003
GNo:G0183093

2002 (3 grants)

Investigation of Protein-Protein Interactions in Bacillus subtillis$10,000
Funding Body: University of Newcastle

Project Team
Professor Peter Lewis, Conjoint Associate Professor Renate Griffith
SchemeRole
Project GrantChief Investigator
Total AmountFunding StartFunding Finish
$10,00020022002
GNo:G0181282

Subcellular localisation of translation factors in Bacillus subtillis$5,000
Funding Body: University of Newcastle

Project Team
Professor Peter Lewis
SchemeRole
Project GrantChief Investigator
Total AmountFunding StartFunding Finish
$5,00020022002
GNo:G0181368

Prokaryotic Development Canada, 10-14 July, 2002$2,400
Funding Body: University of Newcastle

Project Team
Professor Peter Lewis
SchemeRole
Travel GrantChief Investigator
Total AmountFunding StartFunding Finish
$2,40020022002
GNo:G0182003

2001 (3 grants)

Characterisation of transcription foci, their role in chromosome segregation, and the localisation of transcription and translation factors in Bacillus subtilis$211,350
Funding Body: ARC (Australian Research Council)

Project Team
Professor Peter Lewis
SchemeRole
Large GrantChief Investigator
Total AmountFunding StartFunding Finish
$211,35020012003
GNo:G0179272

Subcellular localisation of energy generating enzymes in the bacterium Bacillus subtilis.$12,000
Funding Body: University of Newcastle

Project Team
Professor Peter Lewis
SchemeRole
Project GrantChief Investigator
Total AmountFunding StartFunding Finish
$12,00020012001
GNo:G0179953

ComBio 2001 National Convention Centres, Canberra 1-4 October, 2001$806
Funding Body: University of Newcastle

Project Team
Professor Peter Lewis
SchemeRole
Travel GrantChief Investigator
Total AmountFunding StartFunding Finish
$80620012001
GNo:G0181575

2000 (1 grants)

High resolution microscopic studies on the internal organisation of the bacterium Bacillus subtilis.$15,000
Funding Body: University of Newcastle

Project Team
Professor Peter Lewis
SchemeRole
New Staff GrantChief Investigator
Total AmountFunding StartFunding Finish
$15,00020002000
GNo:G0179918
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Research Supervision

Number of current supervisions6
Total current UoN PhD EFTSL2.5

For supervisions undertaken at an institution other that the University of Newcastle, the institution name is listed below the program name.

Current Supervision

CommencedProposed
Completion
ProgramSupervisor TypeResearch Title
20132017PhD (Biological Sciences)Co-SupervisorUnderstanding Chromosome Dimer Resolution Systems of a Pathogenic Bacteria at a Molecular Level
20132017PhD (Biological Sciences)Co-SupervisorDNA Replication Fork Processing and Recovery in Escherichia Coli
20122016PhD (Biological Sciences)Principal SupervisorBacterial Transcription Factors NusB, NusE ATFA and p as Targets for Antibiotic Research
20122016PhD (Biological Sciences)Co-SupervisorDevelopment of Genetic Tools for the Quantitative Assessment of Gene Depletion in Pathogenic Bacteria
20102014PhD (Biological Sciences)Principal SupervisorIncreased Understanding of the Molecular Interactions Involved in Bacterial Transcription and Recombination
20062009PHD Microbiology
University of Newcastle
Sole SupervisorStructural analysis of RNA polymerase

Past Supervision

YearProgramSupervisor TypeResearch Title
2013PhD (Biological Sciences)Principal SupervisorInvestigation of the Transcription Complex in Acinetobacter baylyi ADP1 and the Identification of the Novel Small Acidic Transcription Factor AtfA
2013PhD (Biological Sciences)Co-SupervisorGenetic Regulation of Embryo Development and Formation of Seed Storage Products in the Legume Model Medicago truncatula
2012PhD (Biological Sciences)Sole SupervisorThe Characterisation of the Interaction between PcrA and RNA Polymerase
2011PhD (Biological Sciences)Principal SupervisorThe RNA Polymerase-Q Interaction as a Target for Potential Novel Antimicrobials
2010PhD (Biological Sciences)Sole SupervisorThe Structure of Bacterial RNA Polymerase in Complex With the Essential Transcription Elongation Factor NusA
2008PhD (Biological Sciences)Sole SupervisorThe Composition of Transcription Complexes in Bacillus Subtilis
2007Honours Microbiology
University of Newcastle
Sole SupervisorInteraction of C-terminus of PcrA with the B subunit of RNA polymerase
2007Honours Microbiology
University of Newcastle
Sole SupervisorCharacterisation of the interaction between PcrA and the B-subunit of RNA polymerase
2006Honours Microbiology
University of Newcastle
Sole SupervisorInteraction of transcription factors NusB and NusE in Bacillus subtilis
2006Honours Microbiology
University of Newcastle
Sole SupervisorCharacterisation of the interaction between PcrA and the beta subunit of RNA polymerase
2006Honours Microbiology
University of Newcastle
Sole SupervisorThe design and testing of inhibitors of interactions between essential transcription factors and RNA polymerase
2005Honours Microbiology
University of Newcastle
Sole SupervisorDevelopment of an engineered Bacillus subtilis strain for the detection of bioavailable environmental toxicants
2004Honours Microbiology
University of Newcastle
Sole SupervisorInteractions between RNA polymerase and transcription factors: An integrated approach
2004Honours Microbiology
University of Newcastle
Sole SupervisorAnalysis of translation initiation factors in Bacillus subtilis
2004Honours Microbiology
University of Newcastle
Sole SupervisorAnalysis of translation elongation factors EF-Tu and EF-G in Bacillus subtilis
2004Honours Microbiology
University of Newcastle
Sole SupervisorSubcellular localisation of the transcription elongation factors NusB, NusG and GreA in Bacillus subtilis
2002Honours Microbiology
University of Newcastle
Sole SupervisorSubcellular localisation of tranlsation factors in Bacillus subtilis
2002Honours Microbiology
University of Newcastle
Sole SupervisorSubcellular localisation of NusA in Bacillus subtilis
2002Honours Microbiology
University of Newcastle
Sole SupervisorSubcellular localisation of ATP synthase in Bacillus subtilis
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Professor Peter Lewis

Work Phone(02) 4921 5701
Fax(02) 4921 5472
Email
PositionProfessor
School of Environmental and Life Sciences
Faculty of Science and Information Technology
The University of Newcastle, Australia
Focus AreaBiological Sciences
Office
BG10,
Biological Sciences,
Callaghan
University Drive
Callaghan NSW 2308
Australia
URL:www.newcastle.edu.au/profile/peter-lewis