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Professor Peter Lewis

Professor

School of Environmental and Life Sciences (Biological Sciences)

Career Summary

Biography

My research career has focused on the gram positive bacterium Bacillus subtilis. During my PhD I purified and characterised the replication terminator protein (RTP) and its DNA binding sites (Lewis et al., J. Bacteriol 171, 3564-3567 (1989); Lewis et al., J. Mol. Biol. 214, 73-84 (1990)). These sites are regions of DNA where oppositely moving replication forks meet and newly replicated chromosomes are resolved prior to segregation. During my post-doctoral research I was responsible for the development of cell biological techniques and vectors for visualising gene expression and protein localisation in live bacterial cells (Lewis et al., PNAS 91, 3849-3853 (1994); Lewis et al., Mol Microbiol 13, 655-662 (1994); Lewis and Errington, Microbiology 142, 733-740 (1996); Lewis and Marston, Gene 227, 101-109 (1999); Feucht and Lewis, Gene 264, 289-297 (2001)).

These techniques have been widely adopted by research groups involved in microbial cell biology, and the plasmid vectors have now been distributed directly to well over 100 laboratories world-wide, although they are now also distributed by the Bacillus Genetic Stock Centre. These techniques were instrumental in determining the establishment of compartment specificity of developmentally regulated s-factors during sporulation in B. subtilis (Lewis et al., PNAS 91, 3849-3853 (1994)). I also showed that prespore specific accumulation of the transcription regulator SpoIIAA was responsible for initiation of compartment-specific gene expression during development, that this accumulation was probably due to prespore-specific activity of the phosphatase SpoIIE, and that a programme of proteolysis was initiated following this activation event (Lewis et al., Genes Cell 1, 881-894 (1996); Lewis et al., J. Bacteriol 180, 3276-3284 (1998)). I was also involved in work that showed that the highly conserved SpoIIIE (FtsK) protein is a DNA translocase that moves DNA through a division septum (Wu et al., Genes Dev 9, 1316-1326 (1995)). This was the first example of such a phenomenon, and was a very significant finding as previously it was assumed that DNA was segregated into daughter cells/different compartments prior to division septum formation. Finally, I have shown that transcription and translation are spatially separated within bacteria (Lewis et al., EMBO J 19, 710-718 (2000)). This was an unexpected result as the 2 processes were thought to be very tightly coupled in bacteria. Furthermore, transcription becomes concentrated into a sub-fraction of the bacterial nucleoid at higher growth rates. These transcription foci have been shown to be the sites of rRNA synthesis, and my laboratory is now focussing on characterising transcription complexes and their structure in detail.

Research Expertise
My research career has focused on the gram positive bacterium Bacillus subtilis. During my PhD I purified and characterised the replication terminator protein (RTP) and its DNA binding sites (Lewis et al., J. Bacteriol 171, 3564-3567 (1989); Lewis et al., J. Mol. Biol. 214, 73-84 (1990)). These sites are regions of DNA where oppositely moving replication forks meet and newly replicated chromosomes are resolved prior to segregation. During my post-doctoral research I was responsible for the development of cell biological techniques and vectors for visualising gene expression and protein localisation in live bacterial cells (Lewis et al., PNAS 91, 3849-3853 (1994); Lewis et al., Mol Microbiol 13, 655-662 (1994); Lewis and Errington, Microbiology 142, 733-740 (1996); Lewis and Marston, Gene 227, 101-109 (1999); Feucht and Lewis, Gene 264, 289-297 (2001)). These techniques have been widely adopted by research groups involved in microbial cell biology, and the plasmid vectors have now been distributed directly to well over 100 laboratories world-wide, although they are now also distributed by the Bacillus Genetic Stock Centre. These techniques were instrumental in determining the establishment of compartment specificity of developmentally regulated s-factors during sporulation in B. subtilis (Lewis et al., PNAS 91, 3849-3853 (1994)). I also showed that prespore specific accumulation of the transcription regulator SpoIIAA was responsible for initiation of compartment-specific gene expression during development, that this accumulation was probably due to prespore-specific activity of the phosphatase SpoIIE, and that a programme of proteolysis was initiated following this activation event (Lewis et al., Genes Cell 1, 881-894 (1996); Lewis et al., J. Bacteriol 180, 3276-3284 (1998)). I was also involved in work that showed that the highly conserved SpoIIIE (FtsK) protein is a DNA translocase that moves DNA through a division septum (Wu et al., Genes Dev 9, 1316-1326 (1995)). This was the first example of such a phenomenon, and was a very significant finding as previously it was assumed that DNA was segregated into daughter cells/different compartments prior to division septum formation. Finally, I have shown that transcription and translation are spatially separated within bacteria (Lewis et al., EMBO J 19, 710-718 (2000)). This was an unexpected result as the 2 processes were thought to be very tightly coupled in bacteria. Furthermore, transcription becomes concentrated into a sub-fraction of the bacterial nucleoid at higher growth rates. These transcription foci have been shown to be the sites of rRNA synthesis, and my laboratory is now focussing on characterising transcription complexes and their structure in detail.

Teaching Expertise
Molecular biology, particularly bacterial. Microbiology.

Administrative Expertise
No expertise.



Qualifications

  • Doctor of Philosophy, University of Sydney
  • Bachelor of Science, University of Sheffield - UK

Keywords

  • Microscopy
  • Molecular Biology
  • Molecular Microbiology
  • Transcription regulation

Fields of Research

CodeDescriptionPercentage
060199Biochemistry and Cell Biology not elsewhere classified50
060499Genetics not elsewhere classified15
110899Medical Microbiology not elsewhere classified35

Professional Experience

UON Appointment

DatesTitleOrganisation / Department
1/01/2014 - ProfessorUniversity of Newcastle
School of Environmental and Life Sciences
Australia

Academic appointment

DatesTitleOrganisation / Department
1/07/2005 - Membership - National Convener Molecular Microbiology Special Interest GroupAustralian Society for Microbiology
Australia
1/07/2000 - Senior LecturerUniversity of Newcastle
School of Environmental and Life Sciences
Australia

Membership

DatesTitleOrganisation / Department
Member of EU 6th Framework Consortium for A$20M EU 6th Framework BaSysBio ProjectEuropean Union (EU) Framework Collaboration
Australia
OzReaderARC Biological Sciences Committee
Australia

Awards

Research Award

YearAward
2004International Short-Term Fellowship
Australian Academy of Sciences

Invitations

Participant

YearTitle / Rationale
2007Invited speaker
Organisation: Conference Description: Invited to speak at SGM 162nd Meeting-Edinburgh International Conference Centre
2007Reviewer
Organisation: Various Publishers in the field of Environmental and Life Science Description: Have been invited to write reviews for top journals in field (Molecular Microbiology, Microbiology and international Review of Cytology) and 2 book chapters in the last 3 years. Work is also now appearing in Microbiology text books.
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Publications

For publications that are currently unpublished or in-press, details are shown in italics.


Chapter (3 outputs)

YearCitationAltmetricsLink
2012Doherty GP, Mettrick KA, Grainge IR, Lewis PJ, 'Imaging fluorescent protein fusions in live bacteria', Methods in Microbiology, Academic Press, Kidlington, Ox 107-126 (2012) [B1]
Co-authorsIan Grainge
2008Lewis PJ, 'Subcellular organisation in bacteria', Bacterial Physiology: A Molecular Approach, Springer, Berlin 1-42 (2008) [B1]
2007Lewis PJ, 'The organization of transcription and translation', Bacillus: Cellular and Molecular Biology, Caister Academic Press, Norfolk, United Kingdom 135-166 (2007) [B1]

Journal article (50 outputs)

YearCitationAltmetricsLink
2015Yang X, Ma C, Lewis PJ, 'Identification of inhibitors of bacterial RNA polymerase.', Methods, (2015)
DOI10.1016/j.ymeth.2015.05.005Author URL
2015Mielczarek M, Thomas RV, Ma C, Kandemir H, Yang X, Bhadbhade M, et al., 'Synthesis and biological activity of novel mono-indole and mono-benzofuran inhibitors of bacterial transcription initiation complex formation', Bioorganic and Medicinal Chemistry, (2015)

Our ongoing research focused on targeting transcription initiation in bacteria has resulted in synthesis of several classes of mono-indole and mono-benzofuran inhibitors that targeted the essential protein-protein interaction between RNA polymerase core and s 70/s A factors in bacteria. In this study, the reaction of indole-2-, indole-3-, indole-7- and benzofuran-2-glyoxyloyl chlorides with amines and hydrazines afforded a variety of glyoxyloylamides and glyoxyloylhydrazides. Similarly, condensation of 2- and 7-trichloroacetylindoles with amines and hydrazines delivered amides and hydrazides. The novel molecules were found to inhibit the RNA polymerase-s 70/s A interaction as measured by ELISA, and also inhibited the growth of both Gram-positive and Gram-negative bacteria in culture. Structure-activity relationship (SAR) studies of the mono-indole and mono-benzofuran inhibitors suggested that the hydrophilic-hydrophobic balance is an important determinant of biological activity.

DOI10.1016/j.bmc.2015.02.037
2015Mielczarek M, Thomas RV, Ma C, Kandemir H, Yang X, Bhadbhade M, et al., 'Synthesis and biological activity of novel mono-indole and mono-benzofuran inhibitors of bacterial transcription initiation complex formation', Bioorganic and Medicinal Chemistry, 23 1763-1775 (2015)

Our ongoing research focused on targeting transcription initiation in bacteria has resulted in synthesis of several classes of mono-indole and mono-benzofuran inhibitors that targeted the essential protein-protein interaction between RNA polymerase core and s70/sA factors in bacteria. In this study, the reaction of indole-2-, indole-3-, indole-7- and benzofuran-2-glyoxyloyl chlorides with amines and hydrazines afforded a variety of glyoxyloylamides and glyoxyloylhydrazides. Similarly, condensation of 2- and 7-trichloroacetylindoles with amines and hydrazines delivered amides and hydrazides. The novel molecules were found to inhibit the RNA polymerase-s70/sA interaction as measured by ELISA, and also inhibited the growth of both Gram-positive and Gram-negative bacteria in culture. Structure-activity relationship (SAR) studies of the mono-indole and mono-benzofuran inhibitors suggested that the hydrophilic-hydrophobic balance is an important determinant of biological activity.

DOI10.1016/j.bmc.2015.02.037
2015Ma C, Mobli M, Yang X, Keller AN, King GF, Lewis PJ, 'RNA polymerase-induced remodelling of NusA produces a pause enhancement complex.', Nucleic Acids Res, 43 2829-2840 (2015)
DOI10.1093/nar/gkv108Author URL
2015Yin Z, Whittell LR, Wang Y, Jergic S, Ma C, Lewis PJ, et al., 'Bacterial Sliding Clamp Inhibitors that Mimic the Sequential Binding Mechanism of Endogenous Linear Motifs.', J Med Chem, 58 4693-4702 (2015)
DOI10.1021/acs.jmedchem.5b00232Author URL
2015Bi Y, Yang X, Zhang T, Liu Z, Zhang X, Lu J, et al., 'Design, synthesis, nitric oxide release and antibacterial evaluation of novel nitrated ocotillol-type derivatives.', Eur J Med Chem, 101 71-80 (2015)
DOI10.1016/j.ejmech.2015.06.021Author URL
2015Bi Y, Ma C, Zhou Z, Zhang T, Zhang H, Zhang X, et al., 'Synthesis and antibacterial evaluation of novel hydrophilic ocotillol-type triterpenoid derivatives from 20(S)-protopanaxadiol', Records of Natural Products, 9 356-368 (2015)

Triterpenoid saponins are involved in plant defense systems to inhibit bacterial invasion. A new series of hydrophilic ocotillol-type triterpenoid derivatives 5-26 have been synthesized with antibacterial activity against Gram-positive bacteria, including a community associated methicillin-resistant Staphylococcus aureus (CA-MRSA; strain USA300). From this series, compounds 6 and 15 were found to be the most active, both with MIC values of 2 µg/mL against B. subtilis 168 and 8 µg/mL against S. aureus USA300, respectively. Furthermore, subsequent assays showed that compounds 6 and 15 displayed strong synergistic effects at sub-MIC levels against both S. aureus USA300 and B. subtilis 168 when combined with two commercial antibiotics, kanamycin and chloramphenicol. Preliminary structure-activity relationship studies were also performed.

2014Keller AN, Yang X, Wiedermannová J, Delumeau O, Krásný L, Lewis PJ, 'e, a new subunit of RNA polymerase found in gram-positive bacteria.', J Bacteriol, 196 3622-3632 (2014) [C1]
DOI10.1128/JB.02020-14Author URL
CitationsScopus - 1Web of Science - 1
2014Withers R, Doherty GP, Jordan M, Yang X, Dixon NE, Lewis PJ, 'AtfA, a new factor in global regulation of transcription in Acinetobacter spp', Molecular Microbiology, 93 1130-1143 (2014) [C1]

Acinetobacter species are widely distributed bacteria in the environment, and have recently gained notoriety as opportunistic nosocomial pathogens. Here we characterize a novel RNA polymerase-interacting protein named acidic transcription factor A, AtfA. It is small and highly acidic, and is widely distributed throughout the ¿ proteobacteria, including other significant pathogens in the genera Moraxella, Pseudomonas, Legionella and Vibrio. In the model species A. baylyiADP1, deletion of atfA significantly affects expression of over 500 genes, resulting in a large cell phenotype, reduced cell fitness, impaired biofilm formation and twitching motility, and increased sensitivity to antibiotics. Deletion of atfA also causes dramatically enhanced sensitivity to ethanol, which is an important growth promoter and virulence factor in Acinetobacter spp. The results suggest that auxiliary factors of RNA polymerase with important biological roles remain to be discovered.

DOI10.1111/mmi.12723
CitationsScopus - 1Web of Science - 1
2014Yang X, Ma C, Lewis P, 'A vector system that allows simple generation of mutant Escherichia coli RNA polymerase', PLASMID, 75 37-41 (2014) [C1]
DOI10.1016/j.plasmid.2014.06.002Author URL
CitationsWeb of Science - 1
2014Yang X, Ma C, Lewis P, 'A vector system that allows simple generation of mutant Escherichia coli RNA polymerase', Plasmid, 75 37-41 (2014) [C1]

We describe a dual vector-based system for overproduction of recombinant Escherichia coli RNA polymerase (RNAP). A cleavable deca-histidine tag (His10) was incorporated into the C-terminus of the ß' subunit to facilitate protein purification. Unique restriction sites were introduced into the genes encoding the ß and ß' subunits (rpoB and rpoC, respectively), facilitating mutation of functionally significant subunit fragments through insertion of modified PCR fragments into the appropriate vector. RNAP with an R275A substitution in the ß' subunit, which is essential for interaction with transcription initiation factor s, was generated and exhibited reduced activity compared to native recombinant RNAP.

DOI10.1016/j.plasmid.2014.06.002
2014Withers R, Doherty GP, Jordan M, Yang X, Dixon NE, Lewis PJ, 'AtfA, a new factor in global regulation of transcription in Acinetobacter spp.', MOLECULAR MICROBIOLOGY, 93 1130-1143 (2014) [C1]
DOI10.1111/mmi.12723Author URL
CitationsWeb of Science - 1
2014Withers R, Doherty GP, Jordan M, Yang X, Dixon NE, Lewis PJ, 'AtfA, a new factor in global regulation of transcription in Acinetobacter spp', Molecular Microbiology, 93 1130-1143 (2014) [C1]

Acinetobacter species are widely distributed bacteria in the environment, and have recently gained notoriety as opportunistic nosocomial pathogens. Here we characterize a novel RNA polymerase-interacting protein named acidic transcription factor A, AtfA. It is small and highly acidic, and is widely distributed throughout the ¿ proteobacteria, including other significant pathogens in the genera Moraxella, Pseudomonas, Legionella and Vibrio. In the model species A. baylyiADP1, deletion of atfA significantly affects expression of over 500 genes, resulting in a large cell phenotype, reduced cell fitness, impaired biofilm formation and twitching motility, and increased sensitivity to antibiotics. Deletion of atfA also causes dramatically enhanced sensitivity to ethanol, which is an important growth promoter and virulence factor in Acinetobacter spp. The results suggest that auxiliary factors of RNA polymerase with important biological roles remain to be discovered.

DOI10.1111/mmi.12723
CitationsScopus - 1
2014Kandemir H, Kutty SK, Black DS, Kumar N, Ma C, Lewis PJ, Griffith R, 'Synthesis and biological evaluation of 2,5-di(7-indolyl)-1,3,4-oxadiazoles, and 2- and 7-indolyl 2-(1,3,4-thiadiazolyl)ketones as antimicrobials', Bioorganic and Medicinal Chemistry, (2014) [C1]

A range of novel hydrazine bridged bis-indoles was prepared from readily available indole-7-glyoxyloylchlorides and 7-trichloroacetylindoles and underwent cyclodehydration to produce 2,5-di(7-indolyl)-1,3,4-oxadiazoles and a 2,2'-bi-1,3,4-oxadiazolyl with phosphoryl chloride in ethyl acetate. This efficient protocol was subsequently used for the synthesis of 2- and 7-indolyl 2-(1,3,4-thiadiazolyl)ketones from related indolyl-hydrazine carbothioamides. The synthesised bis-indoles were evaluated for their antimicrobial properties, particularly the inhibition of protein-protein complex formation between RNA polymerase and s factor and their bactericidal effect on Gram positive Bacillus subtilis and Gram negative Escherichia coli. © 2014 Elsevier Ltd. All rights reserved.

DOI10.1016/j.bmc.2014.01.025
CitationsScopus - 1Web of Science - 1
2014Mielczarek M, Devakaram RV, Ma C, Yang X, Kandemir H, Purwono B, et al., 'Synthesis and biological activity of novel bis-indole inhibitors of bacterial transcription initiation complex formation', Organic and Biomolecular Chemistry, 12 2882-2894 (2014) [C1]

The increasing resistance of bacteria against clinically approved antibiotics is resulting in an alarming decrease in therapeutic options for today's clinicians. We have targeted the essential interaction between bacterial RNA polymerase and s70/sA for the development of lead molecules exhibiting a novel mechanism of antibacterial activity. Several classes of structurally related bis-indole inhibitors of bacterial transcription initiation complex formation were synthesized and their antimicrobial activities were evaluated. Condensation of indole-7- and indole-2-carbohydrazides with 7- and 2-trichloroacetylindoles or indole-7- and indole-2-glyoxyloyl chlorides resulted in the successful synthesis of 7,7'-, 2,2'-, 2,7'- and 3,2'-linked bis-indole derivatives with -CO-NH-NH-CO- and -CO-CO-NH-NH-CO- linkers. Indole-7-glyoxyloyl chlorides were reacted with hydrazine hydrate in different ratios to afford respective -CO-CO-NH-NH-CO-CO- bis-indole or hydrazide derivatives. The resulting compounds were found to be active against the ß'-CH- s70/sA2.2 interaction in ELISA assays and inhibited the growth of both Gram-positive and Gram-negative bacteria. Structure-activity relationship (SAR) studies were performed in order to identify the structural features of the synthesized inhibitors required for biological activity. This journal is © the Partner Organisations 2014.

DOI10.1039/c4ob00460d
CitationsScopus - 4Web of Science - 3
2014Bi Y, Ma C, Zhang H, Zhou Z, Yang J, Zhang Z, et al., 'Novel 3-Substituted Ocotillol-Type Triterpenoid Derivatives as Antibacterial Candidates', CHEMICAL BIOLOGY & DRUG DESIGN, 84 489-496 (2014) [C1]
DOI10.1111/cbdd.12337Author URL
CitationsScopus - 1Web of Science - 1
2013Zhou Z, Ma C, Zhang H, Bi Y, Chen X, Tian H, et al., 'Synthesis and biological evaluation of novel ocotillol-type triterpenoid derivatives as antibacterial agents', European Journal of Medicinal Chemistry, 68 444-453 (2013) [C1]
DOI10.1016/j.ejmech.2013.07.041Author URL
CitationsScopus - 3Web of Science - 3
2013Ma C, Yang X, Kandemir H, Mielczarek M, Johnston EB, Griffith R, et al., 'Inhibitors of Bacterial Transcription Initiation Complex Formation', ACS Chemical Biology, 8 1972-1980 (2013) [C1]
DOI10.1021/cb400231pAuthor URL
CitationsScopus - 11Web of Science - 10
2013Brzoska AJ, Hassan KA, de Leon EJ, Paulsen IT, Lewis PJ, 'Single-Step Selection of Drug Resistant Acinetobacter baylyi ADP1 Mutants Reveals a Functional Redundancy in the Recruitment of Multidrug Efflux Systems', PLOS ONE, 8 (2013) [C1]
DOI10.1371/journal.pone.0056090Author URL
CitationsScopus - 5Web of Science - 5
2012Buescher JM, Liebermeister W, Jules M, Uhr M, Muntel J, Botella E, et al., 'Global network reorganization during dynamic adaptations of Bacillus subtilis metabolism', Science, 335 1099-1103 (2012) [C1]
DOI10.1126/science.1206871
CitationsScopus - 87Web of Science - 79
2012Nicolas P, Mader U, Dervyn E, Rochat T, Leduc A, Pigeonneau N, et al., 'Condition-dependent transcriptome reveals high-level regulatory architecture in Bacillus subtilis', Science, 335 1103-1106 (2012) [C1]
CitationsScopus - 153
2012Buncherd H, Nessen MA, Nouse N, Stelder SK, Roseboom W, Dekker HL, et al., 'Selective enrichment and identification of cross-linked peptides to study 3-D structures of protein complexes by mass spectrometry', Journal of Proteomics, 75 2205-2215 (2012) [C1]
DOI10.1016/j.jprot.2012.01.025
CitationsScopus - 7Web of Science - 8
2011Liew ATF, Theis T, Jensen SO, Garcia-Lara J, Foster SJ, Firth N, et al., 'A simple plasmid-based system that allows rapid generation of tightly controlled gene expression in Staphylococcus aureus', Microbiology, 157 666-676 (2011) [C1]
DOI10.1099/mic.0.045146-0
CitationsScopus - 10Web of Science - 10
2010Yang X, Lewis PJ, 'The interaction between RNA polymerase and the elongation factor NusA', RNA Biology, 7 272-275 (2010) [C1]
CitationsScopus - 8Web of Science - 9
2010Yang X, Lewis PJ, 'The interaction between bacterail transcription factors and RNA polymerase during the transition from initiation to elongation', Transcription, 1 1-4 (2010) [C1]
DOI10.4161/trns.1.2.12791
CitationsScopus - 4
2010Doherty GP, Bailey KL, Lewis PJ, 'Stage-specific fluorescence intensity of GFP and mCherry during sporulation in Bacillus Subtilis', BMC Research Notes, 3 1-8 (2010) [C1]
DOI10.1186/1756-0500-3-303
CitationsScopus - 6
2010Robinson A, Brzoska AJ, Turner KM, Withers RW, Harry EJ, Lewis PJ, Dixon NE, 'Essential biological processes of an emerging pathogen: DNA replication, transcription, and cell division in Acinetobacter spp', Microbiology and Molecular Biology Reviews, 74 273-297 (2010) [C1]
DOI10.1128/MMBR.00048-09
CitationsScopus - 20Web of Science - 23
2010Botella E, Fogg M, Jules M, Piersma S, Doherty GP, Hansen A, et al., 'pBaSysBioII: An integrative plasmid generating gfp transcriptional fusions for high-throughput analysis of gene expression in Bacillus subtilis', Microbiology, 156 1600-1608 (2010) [C1]
DOI10.1099/mic.0.035758-0
CitationsScopus - 29Web of Science - 29
2010Doherty GP, Fogg MJ, Wilkinson AJ, Lewis PJ, 'Small subunits of RNA polymerase: Localization, levels and implications for core enzyme composition', Microbiology, 16 3532-3543 (2010) [C1]
DOI10.1099/mic.0.041566-0
CitationsScopus - 13Web of Science - 13
2009Yang X, Molimau S, Doherty GP, Johnston EB, Marles-Wright J, Rothnagel R, et al., 'The structure of bacterial RNA polymerase in complex with the essential transcription elongation factor NusA', EMBO Reports, 10 997-1002 (2009) [C1]
DOI10.1038/embor.2009.155
CitationsScopus - 23Web of Science - 24
2009Johnston EB, Lewis PJ, Griffith R, 'The interaction of Bacillus subtilis sigma(A) with RNA polymerase', Protein Science, 18 2287-2297 (2009) [C1]
DOI10.1002/pro.239
CitationsScopus - 12Web of Science - 12
2008Yang X, Lewis PJ, 'Overproduction and purification of recombinant Bacillus subtilis RNA polymerase', Protein Expression and Purification, 59 86-93 (2008) [C1]
DOI10.1016/j.pep.2008.01.006
CitationsScopus - 8Web of Science - 11
2008Meredith DH, Plank MW, Lewis PJ, 'Different patterns of integral membrane protein localization during cell division in Bacillus subtilis', Microbiology, 154 64-71 (2008) [C1]
DOI10.1099/mic.0.2007/013268-0
CitationsScopus - 4Web of Science - 4
2008Lewis PJ, Doherty GP, Clarke J, 'Transcription factor dynamics', Microbiology, 154 1837-1844 (2008) [C1]
DOI10.1099/mic.0.2008/018549-0
CitationsScopus - 5Web of Science - 6
2008Huo N, Lewis PJ, 'Green fluorescent protein labeling of ylyA gene in Bacillus subtilis', Weishengwu Xuebao, 48 829-833 (2008) [C1]
2008Marles-Wright J, Grant T, Delumeau O, Van Duinen G, Firbank SJ, Lewis PJ, et al., 'Molecular architecture of the 'stressosome,' a signal integration and transduction hub', Science, 322 92-96 (2008) [C1]
DOI10.1126/science.1159572
CitationsScopus - 64Web of Science - 61
2008Yang X, Doherty GP, Lewis PJ, 'Tandem affinity purification vectors for use in gram positive bacteria', Plasmid, 59 54-62 (2008) [C1]
DOI10.1016/j.plasmid.2007.11.001
CitationsScopus - 2Web of Science - 2
2006Doherty GP, Meredith DH, Lewis PJ, 'Subcellular partitioning of transcription factors in Bacillus subtilis', Journal of Bacteriology, 188 4101-4110 (2006) [C1]
DOI10.1128/JB.01934-05
CitationsScopus - 11Web of Science - 12
2005Davies KM, Dedman AJ, van Horck S, Lewis PJ, 'The NusA:RNA polymerase ratio is increased at sites of rRNA synthesis in Bacillus subtilis.', Mol Microbiol, 57 366-379 (2005)
DOI10.1111/j.1365-2958.2005.04469.xAuthor URL
2005MacDougall IJA, Lewis PJ, Griffith R, 'Homology modelling of RNA polymerase and associated transcription factors from Bacillus subtilis', Journal of Molecular Graphics & Modelling, 23 297-303 (2005) [C1]
DOI10.1016/j.jmgm.2004.10.001
CitationsScopus - 5Web of Science - 6
2005Davies KM, Dedman AJ, Van Horck SH, Lewis PJ, 'The NusA : RNA polymerase ratio is increased at sites of rRNA synthesis in Bacillus subtilis', Molecular Microbiology, 57 366-379 (2005) [C1]
DOI10.1111/j.1365-2958.2005.04669.x
CitationsScopus - 18Web of Science - 20
2004Migocki MD, Lewis PJ, Wake RG, Harry EJ, 'The midcell replication factory in Bacillus subtilis is highly mobile: implications for coordinating chromosome replication with other cell cycle events', Molecular Microbiology, 54 452-463 (2004) [C1]
DOI10.1111/j.1365-2958.2004.04267.x
CitationsScopus - 38Web of Science - 41
2004Lewis PJ, 'Bacterial subcellular architecture: recent advances and future prospects', Molecular Microbiology, 54 1135-1150 (2004) [C1]
DOI10.1111/j.1365-2958.2004.04343.x
CitationsScopus - 30Web of Science - 32
2004Johnson AS, Van Horck SH, Lewis PJ, 'Dynamic localization of membrance proteins in Bacillus subtilis', Microbiology, 150 2815-2824 (2004) [C1]
DOI10.1099/mic.0.27223-0
CitationsScopus - 31Web of Science - 33
2003Davies KM, Lewis PJ, 'Localization of rRNA Synthesis in Bacillus subtilis: Characterization of Loci Involved in Transcription Focus Formation', Journal of Bacteriology, 185 2346-2353 (2003) [C1]
DOI10.1128/JB.185.7.2346-2353.2003
CitationsScopus - 16Web of Science - 16
2003Harry EJ, Lewis PJ, 'Early targeting of Min proteins to the cell poles in germinated spores of Bacillus subtilis: evidence for division apparatus-independent recruitment of Min proteins to the division site', Molecular Microbiology, 47 37-48 (2003) [C1]
DOI10.1046/j.1365-2958.2003.03253.x
CitationsScopus - 40Web of Science - 41
2001Lewis PJ, 'Bacterial chromosome segregation', Microbiology, 147 519-526 (2001) [C1]
CitationsScopus - 18Web of Science - 19
2001Feucht A, Lewis PJ, 'Improved plasmid vectors for the production of multiple fluorescent protein fusions in Bacillus subtilis', Gene, 264 289-297 (2001) [C1]
CitationsScopus - 44Web of Science - 44
2000Meijer WJJ, Lewis PJ, Errington J, Salas M, 'Dynamic relocalization of phage f29 DNA during replication and the role of the viral protein p16.7', EMBO Journal, 19 4182-4190 (2000)

We have examined the localization of DNA replication of the Bacillus subtilis phage f29 by immunofluorescence. To determine where phage replication was localized within infected cells, we examined the distribution of phage replication proteins and the sites of incorporation of nucleotide analogues into phage DNA. On initiation of replication, the phage DNA localized to a single focus within the cell, nearly always towards one end of the host cell nucleoid. At later stages of the infection cycle, phage replication was found to have redistributed to multiple sites around the periphery of the nucleoid, just under the cell membrane. Towards the end of the cycle, phage DNA was once again redistributed to become located within the bulk of the nucleoid. Efficient redistribution of replicating phage DNA from the initial replication site to various sites surrounding the nucleoid was found to be dependent on the phage protein p16.7.

CitationsScopus - 18
1994LEWIS PJ, NWOGUH CE, BARER MR, HARWOOD CR, ERRINGTON J, 'USE OF DIGITIZED VIDEO MICROSCOPY WITH A FLUOROGENIC ENZYME-SUBSTRATE TO DEMONSTRATE CELL-SPECIFIC AND COMPARTMENT-SPECIFIC GENE-EXPRESSION IN SALMONELLA-ENTERITIDIS AND BACILLUS-SUBTILIS', MOLECULAR MICROBIOLOGY, 13 655-662 (1994)
DOI10.1111/j.1365-2958.1994.tb00459.xAuthor URL
CitationsWeb of Science - 30
Show 47 more journal articles

Conference (21 outputs)

YearCitationAltmetricsLink
2008Brzoska AJ, Turner K, Robinson A, Dixon N, Harry L, Lewis PJ, 'Identification of novel protein-protein interactions in Acinetobacter spp', ASM 2008 Melbourne: Program and Abstracts Book, Melbourne, VIC (2008) [E3]
2008Robinson A, Brzoska AJ, Turner K, Harry L, Lewis PJ, Dixon N, 'DNA replication in pathogenic bacteria: Understanding and disrupting protein-protein interactions', ASM 2008 Melbourne: Program and Abstracts Book, Melbourne, VIC (2008) [E3]
2008Turner K, Brzoska AJ, Robinson A, Firth N, Lewis PJ, Dixon N, Harry L, 'Isolation of novel cell division complexes using tandem affinity purification', ASM 2008 Melbourne: Program and Abstracts Book, Melbourne, VIC (2008) [E3]
2008Withers RW, Brzoska AJ, Lewis PJ, 'Establishment of an in vitro transcription assay for Acinetobacter spp', ASM 2008 Melbourne: Program and Abstracts Book, Melbourne, VIC (2008) [E3]
2008Brzoska AJ, Lewis PJ, 'Identification of novel protein-protein interactions in S. aureus', International Symposium on Staphylococci and Staphylococcal Infections: Abstracts, Cairns, QLD (2008) [E3]
2008Lewis PJ, 'Subcellular localization of transcription factors', Society for General Microbiology Spring Meeting Abstracts, Edinburgh, Scotland (2008) [E3]
2007Johnston EB, Griffith R, Lewis PJ, 'The design and testing of inhibitors of interactions between essential transcription factors and RNA polymerase', 32nd Lorne Conference on Protein Structure and Function. Abstracts, Lorne, Vic (2007) [E3]
2007Lewis PJ, Meredith DH, Plank MW, 'Selective removal of integral membrane proteins from the mid-cell site at the onset of cell division', 4th Conference on Functional Genomics of Gram-Positive Microorganisms. Abstracts, Tirrenia, Pisa, Italy (2007) [E3]
2007Yang X, Lewis PJ, 'Structure of transcription factor NUSA-RNA polymerase complex', ASM 2007 Adelaide Program and Abstracts Book, Adelaide, SA (2007) [E3]
2007Lewis PJ, Doherty GP, Meredith DH, 'Transcription factor dynamics', ASM 2007 Adelaide Program and Abstracts Book, Adelaide, SA (2007) [E3]
2007Harriott K, Lewis PJ, 'Characterisation of the interaction between PcrA and the b sub-unit of RNA polymerase', BacPath 9 : The Molecular Biology of Bacterial Pathogens. Abstracts, Lorne, VIC (2007) [E3]
2007Johnston EB, Griffith R, Lewis PJ, 'The design and testing of inhibitors of interactions between essential transcription factors and RNA polymerase', BacPath 9 : The Molecular Biology of Bacterial Pathogens. Abstracts, Lorne, VIC (2007) [E3]
2004MacDougall IJA, Lewis PJ, Griffith R, 'Molecular modelling of the RNA polymerase complex in Bacillus subtilis', MM2004: 9th Molecular Modelling Workshop, Sydney, Australia (2004) [E3]
2004Lewis PJ, 'Subcellular organization of transcription in bacteria', ASM 2004 Sydney National Conference, Sydney, Australia (2004) [E3]
2004Doherty GP, Meredith DH, Lewis PJ, 'Composition of Transcription complexes in Bacillus subtilis', ASM 2004 Sydney National Conference, Sydney, Australia (2004) [E3]
2004Martin S, MacDougall IJA, Griffith R, Lewis PJ, 'Investigation of the interactions between transcription factor NusA with RNA polymerase in Bacillus subtilis', ASM 2004 Sydney National Conference, Sydney, Australia (2004) [E3]
2004Migocki MD, Lewis PJ, Wake G, Harry E, 'Is chromosome replication involved in the spatial regulation of midcell division in Bacillus subtilis?', ASM 2004 Sydney National Conference, Sydney, Australia (2004) [E3]
2003Davies KM, Dedman A, Walsh AK, Van Horck SH, Lewis PJ, 'The bacterial transcription factor NusA is preferentially recruited to sites of rRNA synthesis: Direct evidence for differential RNAP elongation complex formation in vivo', Functional Genomics of Gram-positive Microorganisms 12th International Conference on Bacilli, Baveno, Italy (2003) [E3]
2002Lewis PJ, Davies KM, 'Analysis of transcription foci in bacillus subtilis', American Society for Microbiology - ASM Conference: Prokaryotic Development, Quebec, CANADA (2002) [E3]
2002Lewis PJ, Harry E, 'Cellular targeting of proteins involved in positioning the division site in bacteria', Proceedings of the Australian Society for Biochemistry and Molecular Biology, Sydney, NSW (2002) [E3]
2001Lewis PJ, 'Compartmentalisation of transcription and translation in bacteria', Australian Society for Biochemistry & Molecular Biology Incorp - Combio 2001, Canberra ACT (2001) [E3]
Show 18 more conferences
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Grants and Funding

Summary

Number of grants45
Total funding$26,063,564

Click on a grant title below to expand the full details for that specific grant.


20141 grants / $20,000

Development of compounds that inhibit transcription initiation $20,000

Funding body: University of Newcastle

Funding bodyUniversity of Newcastle
Project TeamProfessor Peter Lewis
SchemeNear Miss Grant
RoleLead
Funding Start2014
Funding Finish2014
GNoG1301396
Type Of FundingInternal
CategoryINTE
UONY

20133 grants / $53,000

Characterisation of a macromolecular complex essential for rapid microbial cell proliferation$20,000

Funding body: University of Newcastle

Funding bodyUniversity of Newcastle
Project TeamProfessor Peter Lewis, Doctor Xiao Yang
SchemeNear Miss Grant
RoleLead
Funding Start2013
Funding Finish2013
GNoG1300482
Type Of FundingInternal
CategoryINTE
UONY

Characterisation of a macromolecular complex essential for rapid microbial cell proliferation$20,000

Funding body: Hunter Medical Research Institute

Funding bodyHunter Medical Research Institute
Project TeamProfessor Peter Lewis
SchemeNear Miss
RoleLead
Funding Start2013
Funding Finish2013
GNoG1300698
Type Of FundingExternal
CategoryEXTE
UONY

New Drugs to Kill Superbugs$13,000

Funding body: University of Newcastle - Faculty of Science & IT

Funding bodyUniversity of Newcastle - Faculty of Science & IT
Project TeamProfessor Peter Lewis, Professor Adam McCluskey, Doctor Chris Gordon, Doctor Joey Ambrus
SchemeStrategic Small Grant
RoleLead
Funding Start2013
Funding Finish2013
GNoG1401065
Type Of FundingInternal
CategoryINTE
UONY

20123 grants / $323,426

Targeting nucleic acid synthesis and cell division in gram-negative bacterial pathogens$311,439

Funding body: NHMRC (National Health & Medical Research Council)

Funding bodyNHMRC (National Health & Medical Research Council)
Project TeamProfessor Nicholas Dixon, Associate Professor Elizabeth Harry, Professor Peter Lewis, Associate Professor Aaron Oakley, Doctor Ian Grainge
SchemeProject Grant
RoleLead
Funding Start2012
Funding Finish2012
GNoG1101133
Type Of FundingAust Competitive - Commonwealth
Category1CS
UONY

Ultrasonic Homogenizer System and -80 ºC Freezers for chemical and biological sample storage$10,000

Funding body: NHMRC (National Health & Medical Research Council)

Funding bodyNHMRC (National Health & Medical Research Council)
Project TeamProfessor Adam McCluskey, Professor Eileen McLaughlin, Professor Peter Lewis, Ms Belinda Nixon, Doctor Shaun Roman, Doctor Jennette Sakoff, Doctor Ian Grainge
SchemeEquipment Grant
RoleInvestigator
Funding Start2012
Funding Finish2012
GNoG1100986
Type Of FundingOther Public Sector - Commonwealth
Category2OPC
UONY

Faculty Visiting Fellowship 2012$1,987

Funding body: University of Newcastle - Faculty of Science & IT

Funding bodyUniversity of Newcastle - Faculty of Science & IT
Project TeamProfessor Peter Lewis
SchemeVisiting Fellowship
RoleLead
Funding Start2012
Funding Finish2012
GNoG1401126
Type Of FundingInternal
CategoryINTE
UONY

20116 grants / $2,011,667

Exploitation of bacterial transcription initiation as a target for new antimicrobials$521,706

Funding body: NHMRC (National Health & Medical Research Council)

Funding bodyNHMRC (National Health & Medical Research Council)
Project TeamProfessor Peter Lewis, Associate Professor Renate Griffith, Associate Professor Naresh Kumar
SchemeProject Grant
RoleLead
Funding Start2011
Funding Finish2011
GNoG1000285
Type Of FundingAust Competitive - Commonwealth
Category1CS
UONY

Establishment of a comprehensive regional biophysical analysis facility $500,000

Funding body: ARC (Australian Research Council)

Funding bodyARC (Australian Research Council)
Project TeamProfessor Peter Lewis, Professor Nicholas Dixon, Professor Eileen McLaughlin, Professor Adam McCluskey, Associate Professor Aaron Oakley, Laureate Professor Paul Foster, Laureate Professor John Aitken, Associate Professor Jennifer Beck, Dr Michael Kelso, Professor Graham Nicholson, Associate Professor Elizabeth Harry, Professor Ian Charles, Associate Professor Steven Djordjevic
SchemeLinkage Infrastructure Equipment & Facilities (LIEF)
RoleLead
Funding Start2011
Funding Finish2011
GNoG1000630
Type Of FundingScheme excluded from IGS
CategoryEXCL
UONY

Chemical Biology$444,961

Funding body: University of Newcastle

Funding bodyUniversity of Newcastle
Project TeamProfessor Adam McCluskey, Doctor Warwick Belcher, Doctor Ian Grainge, Professor Christopher Grof, Professor Peter Lewis, Professor Eileen McLaughlin, Doctor Shaun Roman, Conjoint Professor Ray Rose, Doctor Jennette Sakoff, Doctor Nikki Verrills
SchemePriority Research Centre
RoleInvestigator
Funding Start2011
Funding Finish2011
GNoG1100052
Type Of FundingInternal
CategoryINTE
UONY

Structure function analysis of the NusA-RNA polymerase interaction$300,000

Funding body: ARC (Australian Research Council)

Funding bodyARC (Australian Research Council)
Project TeamProfessor Peter Lewis, Doctor Xiao Yang, Dr Richard Lewis, Dr Jon Marles-Wright
SchemeDiscovery Projects
RoleLead
Funding Start2011
Funding Finish2011
GNoG1000129
Type Of FundingAust Competitive - Commonwealth
Category1CS
UONY

Establishment of a comprehensive regional biophysical analysis facility $230,000

Funding body: ARC (Australian Research Council)

Funding bodyARC (Australian Research Council)
Project TeamProfessor Peter Lewis, Professor Nicholas Dixon, Professor Eileen McLaughlin, Professor Adam McCluskey, Associate Professor Aaron Oakley, Laureate Professor Paul Foster, Laureate Professor John Aitken, Associate Professor Jennifer Beck, Dr Michael Kelso, Professor Graham Nicholson, Associate Professor Elizabeth Harry, Professor Ian Charles, Associate Professor Steven Djordjevic
SchemeLinkage Infrastructure Equipment & Facilities (LIEF) Partner funding
RoleLead
Funding Start2011
Funding Finish2011
GNoG1100484
Type Of FundingScheme excluded from IGS
CategoryEXCL
UONY

Eppendorf mastercycler pro with thermomixer comfort and 5430R centrifuge$15,000

Funding body: NHMRC (National Health & Medical Research Council)

Funding bodyNHMRC (National Health & Medical Research Council)
Project TeamProfessor Eileen McLaughlin, Professor Peter Lewis, Professor Adam McCluskey, Conjoint Professor Keith Jones, Associate Professor Brett Nixon, Doctor Shaun Roman, Doctor Jennette Sakoff, Doctor Ian Grainge, Doctor Janet Holt, Doctor Xiao Yang
SchemeEquipment Grant
RoleInvestigator
Funding Start2011
Funding Finish2011
GNoG1100028
Type Of FundingOther Public Sector - Commonwealth
Category2OPC
UONY

20093 grants / $570,000

LAS-4000 Chemiluminescence imaging system$25,000

Funding body: NHMRC (National Health & Medical Research Council)

Funding bodyNHMRC (National Health & Medical Research Council)
Project TeamProfessor Eileen McLaughlin, Laureate Professor John Aitken, Professor Peter Lewis, Associate Professor Brett Nixon, Doctor Shaun Roman
SchemeEquipment Grant
RoleInvestigator
Funding Start2009
Funding Finish2009
GNoG0189852
Type Of FundingOther Public Sector - Commonwealth
Category2OPC
UONY

20082 grants / $107,600

X-ray crystallography resource for membrane proteins and large macromolecular complexes$93,000

Funding body: ARC (Australian Research Council)

Funding bodyARC (Australian Research Council)
Project TeamProfessor Peter Lewis, Doctor Robyn Malby
SchemeLinkage Infrastructure Equipment & Facilities (LIEF)
RoleLead
Funding Start2008
Funding Finish2008
GNoG0188894
Type Of FundingScheme excluded from IGS
CategoryEXCL
UONY

Structural Analysis of Bacterial Transcription Proteins by X-ray Crystallography$14,600

Funding body: University of Newcastle

Funding bodyUniversity of Newcastle
Project TeamDoctor Robyn Malby, Professor Peter Lewis
SchemePilot Grant
RoleInvestigator
Funding Start2008
Funding Finish2008
GNoG0189075
Type Of FundingInternal
CategoryINTE
UONY

20077 grants / $1,367,901

Nucleic Acid Synthesis and Cell Division in Model Pathogenic Bacteria$740,250

Funding body: NHMRC (National Health & Medical Research Council)

Funding bodyNHMRC (National Health & Medical Research Council)
Project TeamProfessor Peter Lewis, Professor Nicholas Dixon, Associate Professor Elizabeth Harry
SchemeProject Grant
RoleLead
Funding Start2007
Funding Finish2007
GNoG0186390
Type Of FundingAust Competitive - Commonwealth
Category1CS
UONY

Towards an understanding of dynamic genetic regulation at global scale in bacteria: A systems biology approach$486,000

Funding body: NHMRC (National Health & Medical Research Council)

Funding bodyNHMRC (National Health & Medical Research Council)
Project TeamProfessor Peter Lewis
SchemeNHMRC - European Union Collaborative Research Grants
RoleLead
Funding Start2007
Funding Finish2007
GNoG0186507
Type Of FundingAust Competitive - Commonwealth
Category1CS
UONY

Towards an understanding of dynamic transcriptional regulation at a global scale in bacteria: a system biology approach$110,250

Funding body: Department of Innovation, Industry, Science and Research

Funding bodyDepartment of Innovation, Industry, Science and Research
Project TeamProfessor Peter Lewis
SchemeInternational Science Linkages (ISL) Program
RoleLead
Funding Start2007
Funding Finish2007
GNoG0187015
Type Of FundingAust Competitive - Commonwealth
Category1CS
UONY

RNA POLYMERASE PROTEIN INTERACTIONS AND THE REGULATION OF TRANSCRIPTION ELONGATION$16,900

Funding body: University of Newcastle

Funding bodyUniversity of Newcastle
Project TeamProfessor Peter Lewis
SchemeNear Miss Grant
RoleLead
Funding Start2007
Funding Finish2007
GNoG0187186
Type Of FundingInternal
CategoryINTE
UONY

Hamamatsu ORCA-AG cooled CCD camera$12,000

Funding body: NHMRC (National Health & Medical Research Council)

Funding bodyNHMRC (National Health & Medical Research Council)
Project TeamProfessor Peter Lewis
SchemeEquipment Grant
RoleLead
Funding Start2007
Funding Finish2007
GNoG0188190
Type Of FundingOther Public Sector - Commonwealth
Category2OPC
UONY

4th Conference on Functional Genomics of Gram-Positive Microorganisms, Green Park Resort, Tirrenia, Italy, 24/6/2007 - 28/6/2007$2,500

Funding body: University of Newcastle

Funding bodyUniversity of Newcastle
Project TeamProfessor Peter Lewis
SchemeTravel Grant
RoleLead
Funding Start2007
Funding Finish2007
GNoG0187783
Type Of FundingInternal
CategoryINTE
UONY

Towards an understanding of dynamic transcriptional regulation at global scale in bacteria: a systems biology approach. BaSysBio$1

Funding body: European Commission, European Union

Funding bodyEuropean Commission, European Union
Project TeamProfessor Peter Lewis
SchemeSixth Framework Programme
RoleLead
Funding Start2007
Funding Finish2007
GNoG0186876
Type Of FundingNot Known
CategoryUNKN
UONY

20062 grants / $20,273,000

Towards and understanding of dynamic genetic regulation at a global scale in bacteria: a Systems biology approach$20,000,000

Funding body: European Commission, European Union

Funding bodyEuropean Commission, European Union
Project Team
Scheme6th Framework
RoleInvestigator
Funding Start2006
Funding Finish2007
GNo
Type Of FundingInternational - Non Competitive
Category3IFB
UONY

Structural analysis and functional inactivation of bacterial transcription complexes$273,000

Funding body: ARC (Australian Research Council)

Funding bodyARC (Australian Research Council)
Project TeamProfessor Peter Lewis, Conjoint Associate Professor Renate Griffith, Dr Richard Lewis
SchemeDiscovery Projects
RoleLead
Funding Start2006
Funding Finish2006
GNoG0185244
Type Of FundingAust Competitive - Commonwealth
Category1CS
UONY

20055 grants / $274,064

Structural analysis of bacillus subtilis RNA polymerase elongation complexes$25,064

Funding body: ARC (Australian Research Council)

Funding bodyARC (Australian Research Council)
Project TeamProfessor Peter Lewis, Dr Richard Lewis, Assoc. Prof Alasdair McDowall
SchemeLinkage International Award
RoleLead
Funding Start2005
Funding Finish2005
GNoG0184314
Type Of FundingAust Competitive - Commonwealth
Category1CS
UONY

2005 RIBG allocation$20,811

Funding body: University of Newcastle

Funding bodyUniversity of Newcastle
Project TeamProfessor Peter Lewis
SchemeResearch Infrastructure Block Grant (RIBG)
RoleLead
Funding Start2005
Funding Finish2005
GNoG0185832
Type Of FundingInternal
CategoryINTE
UONY

Structural Analysis and Functional Inactivation of Bacillus Subtilis RNA Polymerase$13,000

Funding body: University of Newcastle

Funding bodyUniversity of Newcastle
Project TeamProfessor Peter Lewis, Conjoint Associate Professor Renate Griffith
SchemeProject Grant
RoleLead
Funding Start2005
Funding Finish2005
GNoG0184652
Type Of FundingInternal
CategoryINTE
UONY

Development of genetically engineered systems to monitor the bioavailability of pollutants in soil and water samples$8,000

Funding body: University of Newcastle

Funding bodyUniversity of Newcastle
Project TeamProfessor Peter Lewis
SchemeProject Grant
RoleLead
Funding Start2005
Funding Finish2005
GNoG0184651
Type Of FundingInternal
CategoryINTE
UONY

20044 grants / $795,450

Multifunctional confocal laser scanning microscope with time resolved and two photon imaging and fluorescence correlation capabilities$579,000

Funding body: Australian Research Council

Funding bodyAustralian Research Council
Project Team
SchemeLinkage Infrastructure Equipment & Facilities (LIEF)
RoleInvestigator
Funding Start2004
Funding Finish2004
GNo
Type Of FundingAust Competitive - Commonwealth
Category1CS
UONY

In vivo studies on the regulation of transcription and translation in Bacillus subtilis$210,000

Funding body: ARC (Australian Research Council)

Funding bodyARC (Australian Research Council)
Project TeamProfessor Peter Lewis
SchemeDiscovery Projects
RoleLead
Funding Start2004
Funding Finish2004
GNoG0182839
Type Of FundingAust Competitive - Commonwealth
Category1CS
UONY

Investigation of transcript factor interaction with RNA polymerase in Bacillus subtilis$6,000

Funding body: University of Newcastle

Funding bodyUniversity of Newcastle
Project TeamProfessor Peter Lewis, Conjoint Associate Professor Renate Griffith
SchemeProject Grant
RoleLead
Funding Start2004
Funding Finish2004
GNoG0183378
Type Of FundingInternal
CategoryINTE
UONY

ASM 2004 Sydney, 26 Sept - 1 Oct 2004$450

Funding body: University of Newcastle

Funding bodyUniversity of Newcastle
Project TeamProfessor Peter Lewis
SchemeTravel Grant
RoleLead
Funding Start2004
Funding Finish2004
GNoG0184930
Type Of FundingInternal
CategoryINTE
UONY

20032 grants / $10,900

Protein-protein interactions in Bacillus subtilis.$8,500

Funding body: University of Newcastle

Funding bodyUniversity of Newcastle
Project TeamProfessor Peter Lewis, Conjoint Associate Professor Renate Griffith
SchemeProject Grant
RoleLead
Funding Start2003
Funding Finish2003
GNoG0182487
Type Of FundingInternal
CategoryINTE
UONY

International Conference of Functional Genomics of Gram-positive Microorganisms, Baveno, Italy 22-28 June, 2003$2,400

Funding body: University of Newcastle

Funding bodyUniversity of Newcastle
Project TeamProfessor Peter Lewis
SchemeTravel Grant
RoleLead
Funding Start2003
Funding Finish2003
GNoG0183093
Type Of FundingInternal
CategoryINTE
UONY

20023 grants / $17,400

Investigation of Protein-Protein Interactions in Bacillus subtillis$10,000

Funding body: University of Newcastle

Funding bodyUniversity of Newcastle
Project TeamProfessor Peter Lewis, Conjoint Associate Professor Renate Griffith
SchemeProject Grant
RoleLead
Funding Start2002
Funding Finish2002
GNoG0181282
Type Of FundingInternal
CategoryINTE
UONY

Subcellular localisation of translation factors in Bacillus subtillis$5,000

Funding body: University of Newcastle

Funding bodyUniversity of Newcastle
Project TeamProfessor Peter Lewis
SchemeProject Grant
RoleLead
Funding Start2002
Funding Finish2002
GNoG0181368
Type Of FundingInternal
CategoryINTE
UONY

Prokaryotic Development Canada, 10-14 July, 2002$2,400

Funding body: University of Newcastle

Funding bodyUniversity of Newcastle
Project TeamProfessor Peter Lewis
SchemeTravel Grant
RoleLead
Funding Start2002
Funding Finish2002
GNoG0182003
Type Of FundingInternal
CategoryINTE
UONY

20013 grants / $224,156

Characterisation of transcription foci, their role in chromosome segregation, and the localisation of transcription and translation factors in Bacillus subtilis$211,350

Funding body: ARC (Australian Research Council)

Funding bodyARC (Australian Research Council)
Project TeamProfessor Peter Lewis
SchemeLarge Grant
RoleLead
Funding Start2001
Funding Finish2001
GNoG0179272
Type Of FundingAust Competitive - Commonwealth
Category1CS
UONY

Subcellular localisation of energy generating enzymes in the bacterium Bacillus subtilis.$12,000

Funding body: University of Newcastle

Funding bodyUniversity of Newcastle
Project TeamProfessor Peter Lewis
SchemeProject Grant
RoleLead
Funding Start2001
Funding Finish2001
GNoG0179953
Type Of FundingInternal
CategoryINTE
UONY

ComBio 2001 National Convention Centres, Canberra 1-4 October, 2001$806

Funding body: University of Newcastle

Funding bodyUniversity of Newcastle
Project TeamProfessor Peter Lewis
SchemeTravel Grant
RoleLead
Funding Start2001
Funding Finish2001
GNoG0181575
Type Of FundingInternal
CategoryINTE
UONY

20001 grants / $15,000

High resolution microscopic studies on the internal organisation of the bacterium Bacillus subtilis.$15,000

Funding body: University of Newcastle

Funding bodyUniversity of Newcastle
Project TeamProfessor Peter Lewis
SchemeNew Staff Grant
RoleLead
Funding Start2000
Funding Finish2000
GNoG0179918
Type Of FundingInternal
CategoryINTE
UONY
Edit

Research Supervision

Current Supervision

CommencedResearch Title / Program / Supervisor Type
2015Elucidating the Function of the Novel Transcription Factor AtfA and its Relevance to Virulence and Persistence in the y Proteobacteria
Biological Sciences, Faculty of Science and Information Technology
Principal Supervisor
2013Understanding Chromosome Dimer Resolution Systems of a Pathogenic Bacteria at a Molecular Level
Biological Sciences, Faculty of Science and Information Technology
Co-Supervisor
2012Development of Genetic Tools for the Quantitative Assessment of Gene Depletion in Pathogenic Bacteria
Biological Sciences, Faculty of Science and Information Technology
Co-Supervisor
2012Bacterial Transcription Factors NusB, NusE ATFA and p as Targets for Antibiotic Research
Biological Sciences, Faculty of Science and Information Technology
Principal Supervisor
2006Structural analysis of RNA polymerase
Microbiology, University of Newcastle
Sole Supervisor

Past Supervision

YearResearch Title / Program / Supervisor Type
2014Increased Understanding of the Molecular Interactions Involved in Bacterial Transcription and Recombination
Biological Sciences, Faculty of Science and Information Technology
Principal Supervisor
2013Investigation of the Transcription Complex in Acinetobacter baylyi ADP1 and the Identification of the Novel Small Acidic Transcription Factor AtfA
Biological Sciences, Faculty of Science and Information Technology
Principal Supervisor
2013Genetic Regulation of Embryo Development and Formation of Seed Storage Products in the Legume Model Medicago truncatula
Biological Sciences, Faculty of Science and Information Technology
Co-Supervisor
2012The Characterisation of the Interaction between PcrA and RNA Polymerase
Biological Sciences, Faculty of Science and Information Technology
Sole Supervisor
2011The RNA Polymerase-Q Interaction as a Target for Potential Novel Antimicrobials
Biological Sciences, Faculty of Science and Information Technology
Principal Supervisor
2010The Structure of Bacterial RNA Polymerase in Complex With the Essential Transcription Elongation Factor NusA
Biological Sciences, Faculty of Science and Information Technology
Sole Supervisor
2008The Composition of Transcription Complexes in Bacillus Subtilis
Biological Sciences, Faculty of Science and Information Technology
Sole Supervisor
2007Interaction of C-terminus of PcrA with the B subunit of RNA polymerase
Microbiology, University of Newcastle
Sole Supervisor
2006Characterisation of the interaction between PcrA and the B-subunit of RNA polymerase
Microbiology, University of Newcastle
Sole Supervisor
2005Development of an engineered Bacillus subtilis strain for the detection of bioavailable environmental toxicants
Microbiology, University of Newcastle
Sole Supervisor
2005Characterisation of the interaction between PcrA and the beta subunit of RNA polymerase
Microbiology, University of Newcastle
Sole Supervisor
2005Interaction of transcription factors NusB and NusE in Bacillus subtilis
Microbiology, University of Newcastle
Sole Supervisor
2005The design and testing of inhibitors of interactions between essential transcription factors and RNA polymerase
Microbiology, University of Newcastle
Sole Supervisor
2003Interactions between RNA polymerase and transcription factors: An integrated approach
Microbiology, University of Newcastle
Sole Supervisor
2003Subcellular localisation of the transcription elongation factors NusB, NusG and GreA in Bacillus subtilis
Microbiology, University of Newcastle
Sole Supervisor
2003Analysis of translation elongation factors EF-Tu and EF-G in Bacillus subtilis
Microbiology, University of Newcastle
Sole Supervisor
2003Analysis of translation initiation factors in Bacillus subtilis
Microbiology, University of Newcastle
Sole Supervisor
2002Subcellular localisation of tranlsation factors in Bacillus subtilis
Microbiology, University of Newcastle
Sole Supervisor
2001Subcellular localisation of ATP synthase in Bacillus subtilis
Microbiology, University of Newcastle
Sole Supervisor
2001Subcellular localisation of NusA in Bacillus subtilis
Microbiology, University of Newcastle
Sole Supervisor
Edit

Professor Peter Lewis

Position

Professor
School of Environmental and Life Sciences
Faculty of Science and Information Technology

Focus area

Biological Sciences

Contact Details

Emailpeter.lewis@newcastle.edu.au
Phone(02) 4921 5701
Fax(02) 4921 5472

Office

RoomBG10
BuildingBiological Sciences
LocationCallaghan
University Drive
Callaghan, NSW 2308
Australia
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