2023 |
Bryan ER, Trim LK, Sadowski P, Paramasivan S, Kim JJ, Gough K, et al., 'Prophylactic and therapeutic vaccination protects sperm health from Chlamydia muridarum-induced abnormalities.', Biol Reprod, 108 758-777 (2023) [C1]
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Nova |
2023 |
Frost ER, Ford EA, Peters AE, Lovell-Badge R, Taylor G, McLaughlin EA, Sutherland JM, 'A New Understanding, Guided by Single-Cell Sequencing, of the Establishment and Maintenance of the Ovarian Reserve in Mammals', SEXUAL DEVELOPMENT, 17 145-155 (2023) [C1]
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Nova |
2023 |
Radhakrishnan K, Luu M, Iaria J, Sutherland JM, McLaughlin EA, Zhu HJ, Loveland KL, 'Activin and BMP Signalling in Human Testicular Cancer Cell Lines, and a Role for the Nucleocytoplasmic Transport Protein Importin-5 in Their Crosstalk', Cells, 12 (2023) [C1]
Testicular germ cell tumours (TGCTs) are the most common malignancy in young men. Originating from foetal testicular germ cells that fail to differentiate correctly, TGCTs appear ... [more]
Testicular germ cell tumours (TGCTs) are the most common malignancy in young men. Originating from foetal testicular germ cells that fail to differentiate correctly, TGCTs appear after puberty as germ cell neoplasia in situ cells that transform through unknown mechanisms into distinct seminoma and non-seminoma tumour types. A balance between activin and BMP signalling may influence TGCT emergence and progression, and we investigated this using human cell line models of seminoma (TCam-2) and non-seminoma (NT2/D1). Activin A- and BMP4-regulated transcripts measured at 6 h post-treatment by RNA-sequencing revealed fewer altered transcripts in TCam-2 cells but a greater responsiveness to activin A, while BMP4 altered more transcripts in NT2/D1 cells. Activin significantly elevated transcripts linked to pluripotency, cancer, TGF-ß, Notch, p53, and Hippo signalling in both lines, whereas BMP4 altered TGF-ß, pluripotency, Hippo and Wnt signalling components. Dose-dependent antagonism of BMP4 signalling by activin A in TCam-2 cells demonstrated signalling crosstalk between these two TGF-ß superfamily arms. Levels of the nuclear transport protein, IPO5, implicated in BMP4 and WNT signalling, are highly regulated in the foetal mouse germline. IPO5 knockdown in TCam-2 cells using siRNA blunted BMP4-induced transcript changes, indicating that IPO5 levels could determine TGF-ß signalling pathway outcomes in TGCTs.
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2022 |
Ford EA, Frost ER, Beckett EL, Roman SD, McLaughlin EA, Sutherland JM, 'Transcriptomic profiling of neonatal mouse granulosa cells reveals new insights into primordial follicle activation .', Biol Reprod, 106 503-514 (2022) [C1]
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2022 |
Siddall NA, Casagranda F, Johanson TM, Dominado N, Heaney J, Sutherland JM, et al., 'MiMIC analysis reveals an isoform specific role for Drosophila Musashi in follicle stem cell maintenance and escort cell function', CELL DEATH DISCOVERY, 8 (2022) [C1]
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Nova |
2022 |
Ford EA, Peters AE, Roman SD, McLaughlin EA, Beckett EL, Sutherland JM, 'A scoping review of the information provided by fertility smartphone applications', Human Fertility, 25 625-639 (2022) [C1]
The growth of smartphone application use across areas of female reproductive health has led to increased interest into their functions and benefits. This scoping review aims to de... [more]
The growth of smartphone application use across areas of female reproductive health has led to increased interest into their functions and benefits. This scoping review aims to determine the nature and extent of the peer-reviewed literature presented on fertility-based apps, to identify the reliability of the information within the apps, and to determine the ability of this information to educate users. A systematic search of six databases was conducted in April 2020, returning a total of 21,158 records. After duplicate removal, title and abstract screening exclusionary steps, 27 records were reviewed and charted. Records covered a variety of reproductive health themes including contraception, sexual health, and family planning, and used a range of methodologies. The accuracy of fertility information within the apps reported in these studies was variable, but overall there was a lack of depth in the coverage of content in apps. It was common for studies in this review to base fertile window algorithms on stringent cycle length and variability requirements, limiting the applicability of information delivered to users. Furthermore, studies from app affiliates often lacked collaborations with researchers, minimising the potential for fertility knowledge improvements integrated across the suite of female reproductive health apps.
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Nova |
2022 |
FORD E, PETERS A, ROMAN S, MCLAUGHLIN E, BECKETT E, SUTHERLAND J, 'Studies of Fertility Smartphone Apps Reveal a Lack of Insight and Scientific Information', Fertility & Reproduction, 04 183-183 (2022)
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2022 |
Prokopuk L, Jarred EG, Blücher RO, McLaughlin EA, Stringer JM, Western PS, 'An essential role for Polycomb Repressive Complex 2 in the mouse ovary.', Reproduction, 163 167-182 (2022) [C1]
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Nova |
2021 |
Sutherland JM, McLaughlin EA, 'Ovarian ageing: Where are we now? And where to next?', Current Opinion in Endocrine and Metabolic Research, 18 29-34 (2021) [C1]
From the mid-1980s, the scientific community has demonstrated irrefutable evidence for the age-related decline in female fertility (Menken et al., 1986) [1], supporting observatio... [more]
From the mid-1980s, the scientific community has demonstrated irrefutable evidence for the age-related decline in female fertility (Menken et al., 1986) [1], supporting observations in animals and humans of maternal ageing, increased incidence of miscarriage and congenital abnormality dating back to the mid-20th Century (Warburton, 1970) [2]. Although, it was not until 2005 that a report arising from the European Society of Human Reproduction and Embryology sought to address ovarian ageing as the primary contributing factor to this decline and its profound influence on the success of assisted reproduction. Over the resulting two decades, researchers have endeavoured to define and quantify the genetic, molecular and cellular factors directly responsible for ovarian ageing and subsequently female age-related infertility, seeking to ultimately improve diagnostics and treatments. In this review, we undertake a closer investigation of the ¿usual suspects¿ and speculate on the role of synergistic inflammaging and finally address the question of whether in 2020 we are really any closer to finding the culprits underpinning the ageing ovary.
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2021 |
Peters AE, Caban SJ, McLaughlin EA, Roman SD, Bromfield EG, Nixon B, Sutherland JM, 'The Impact of Aging on Macroautophagy in the Pre-ovulatory Mouse Oocyte', Frontiers in Cell and Developmental Biology, 9 (2021) [C1]
Accompanying the precipitous age-related decline in human female fertility is an increase in the proportion of poor-quality oocytes within the ovary. The macroautophagy pathway, a... [more]
Accompanying the precipitous age-related decline in human female fertility is an increase in the proportion of poor-quality oocytes within the ovary. The macroautophagy pathway, an essential protein degradation mechanism responsible for maintaining cell health, has not yet been thoroughly investigated in this phenomenon. The aim of this study was to characterize the macroautophagy pathway in an established mouse model of oocyte aging using in-depth image analysis-based methods and to determine mechanisms that account for the observed changes. Three autophagy pathway markers were selected for assessment of gene and protein expression in this model: Beclin 1; an initiator of autophagosome formation, Microtubule-associated protein 1 light chain 3B; a constituent of the autophagosome membrane, and lysosomal-associated membrane protein 1; a constituent of the lysosome membrane. Through quantitative image analysis of immunolabeled oocytes, this study revealed impairment of the macroautophagy pathway in the aged oocyte with an attenuation of both autophagosome and lysosome number. Additionally, an accumulation of amphisomes greater than 10 µm2 in area were observed in aging oocytes, and this accumulation was mimicked in oocytes treated with lysosomal inhibitor chloroquine. Overall, these findings implicate lysosomal dysfunction as a prominent mechanism by which these age-related changes may occur and highlight the importance of macroautophagy in maintaining mouse pre-ovulatory oocyte quality. This provides a basis for further investigation of dysfunctional autophagy in poor oocyte quality and for the development of therapeutic or preventative strategies to aid in the maintenance of pre-ovulatory oocyte health.
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Nova |
2021 |
Frost ER, Ford EA, Taylor G, Boeing S, Beckett EL, Roman SD, et al., 'Two alternative methods for the retrieval of somatic cell populations from the mouse ovary', MOLECULAR HUMAN REPRODUCTION, 27 (2021) [C1]
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Nova |
2021 |
Bryan ER, Barrero RA, Cheung E, Tickner JAD, Trim LK, Richard D, et al., 'DNA damage contributes to transcriptional and immunological dysregulation of testicular cells during Chlamydia infection', AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY, 86 (2021) [C1]
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Nova |
2020 |
Frost ER, Ford EA, Peters AE, Reed NL, McLaughlin EA, Baker MA, et al., 'Signal transducer and activator of transcription (STAT) 1 and STAT3 are expressed in the human ovary and have Janus kinase 1-independent functions in the COV434 human granulosa cell line', Reproduction, Fertility and Development, 32 1027-1039 (2020) [C1]
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2020 |
Ford E, Beckett EL, Roman S, McLaughlin EA, Sutherland J, 'Advances in human primordial follicle activation and premature ovarian insufficiency.', Reproduction, 159 R15-R29 (2020) [C1]
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Nova |
2020 |
Frost ER, Ford EA, Peters AE, Reed NL, McLaughlin EA, Baker MA, et al., 'Signal transducer and activator of transcription (STAT) 1 and STAT3 are expressed in the human ovary and have Janus kinase 1-independent functions in the COV434 human granulosa cell line', Reproduction, Fertility and Development, 32 1027-1039 (2020) [C1]
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Nova |
2020 |
Ford EA, Roman SD, McLaughlin EA, Beckett EL, Sutherland JM, 'The association between reproductive health smartphone applications and fertility knowledge of Australian women', BMC Women's Health, 20 (2020) [C1]
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Nova |
2020 |
Bryan ER, Redgrove KA, Mooney AR, Mihalas BP, Sutherland JM, Carey AJ, et al., 'Chronic testicular Chlamydia muridarum infection impairs mouse fertility and offspring development ', Biology of Reproduction, 102 888-901 (2020) [C1]
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Nova |
2020 |
Mihalas BP, Redgrove KA, Bernstein IR, Robertson MJ, McCluskey A, Nixon B, et al., 'Dynamin 2-dependent endocytosis is essential for mouse oocyte development and fertility', FASEB Journal, 34 5162-5177 (2020) [C1]
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Nova |
2019 |
Bryan ER, Kollipara A, Trim LK, Armitage CW, Carey AJ, Mihalas B, et al., 'Hematogenous dissemination of Chlamydia muridarum from the urethra in macrophages causes testicular infection and sperm DNA damage', Biology of Reproduction, 101 748-759 (2019) [C1]
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Nova |
2019 |
Bryan ER, McLachlan R, Rombauts L, Katz DJ, Yazdani A, Bogoevski K, et al., 'Detection of chlamydia infection within human testicular biopsies', HUMAN REPRODUCTION, 34 1891-1898 (2019) [C1]
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Nova |
2019 |
Zhou W, Stanger SJ, Anderson AL, Bernstein IR, De Iuliis GN, McCluskey A, et al., 'Mechanisms of tethering and cargo transfer during epididymosome-sperm interactions.', BMC biology, 17 35-35 (2019) [C1]
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Nova |
2019 |
Bromfield E, Walters JLH, Cafe S, Bernstein I, Stanger SR, Anderson AL, et al., 'Differential cell death decisions in the testis: evidence for an exclusive window of ferroptosis in round spermatids', Molecular Human Reproduction, 25 241-256 (2019) [C1]
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Nova |
2019 |
Mihalas BP, Camlin NJ, Xavier MJ, Peters AE, Holt JE, Sutherland JM, et al., 'The small non-coding RNA profile of mouse oocytes is modified during aging', AGING-US, 11 2968-2997 (2019) [C1]
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Nova |
2018 |
Sutherland JM, Sobinoff AP, Fraser BA, Redgrove KA, Siddall NA, Koopman P, et al., 'RNA binding protein Musashi-2 regulates PIWIL1 and TBX1 in mouse spermatogenesis', JOURNAL OF CELLULAR PHYSIOLOGY, 233 3262-3273 (2018) [C1]
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Nova |
2018 |
Saeidi S, Shapouri F, de Iongh RU, Casagranda F, Sutherland JM, Western PS, et al., 'Esrp1 is a marker of mouse fetal germ cells and differentially expressed during spermatogenesis', PLOS ONE, 13 (2018) [C1]
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Nova |
2018 |
Micati DJ, Hime GR, McLaughlin EA, Abud HE, Loveland KL, 'Differential expression profiles of conserved Snail transcription factors in the mouse testis', ANDROLOGY, 6 362-373 (2018) [C1]
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Nova |
2018 |
Hall SE, Upton RMO, McLaughlin EA, Sutherland JM, 'Phosphoinositide 3-kinase/protein kinase B (PI3K/AKT) and Janus kinase/signal transducer and activator of transcription (JAK/STAT) follicular signalling is conserved in the mare ovary', Reproduction, Fertility and Development, 30 624-633 (2018) [C1]
The mare ovary is unique in its anatomical structure; however, the signalling pathways responsible for physiological processes, such as follicular activation, remain uncharacteris... [more]
The mare ovary is unique in its anatomical structure; however, the signalling pathways responsible for physiological processes, such as follicular activation, remain uncharacterised. This provided us with the impetus to explore whether signalling molecules from important folliculogenesis pathways, phosphoinositide 3-kinase/protein kinase B (PI3K/AKT) and Janus kinase/signal transducer and activator of transcription (JAK/STAT), are conserved in the mare ovary. Messenger RNA expression of six genes important in follicle development was measured using quantitative polymerase chain reaction and protein localisation of key pathway members (PI3K, AKT1, phosphatase and tensin homologue (PTEN), JAK1, STAT3 and suppressor of cytokine signalling 4 (SOCS4)) was compared in tissue from fetal and adult mare ovaries. Tissue from adult ovaries exhibited significantly increased levels of mRNA expression of PI3K, AKT1, PTEN, JAK1, STAT3 and SOCS4 compared with tissue from fetal ovaries. PI3K, AKT1, JAK1 and STAT3 demonstrated redistributed localisation, from pregranulosa cells in fetal development, to both the oocyte and granulosa cells of follicles in the adult ovary, whilst negative feedback molecules PTEN and SOCS4 were only localised to the granulosa cells in the adult ovary. These findings suggest that the PI3K/AKT and JAK/STAT signalling pathways are utilised during folliculogenesis in the mare, similarly to previously studied mammalian species, and may serve as useful biomarkers for assessment of ovary development in the horse.
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Nova |
2018 |
Sutherland JM, Frost ER, Ford EA, Peters AE, Reed NL, Seldon AN, et al., 'Janus Kinase JAK1 maintains the ovarian reserve of primordial follicles in the mouse ovary.', Molecular Human Reproduction, 24 533-542 (2018) [C1]
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Nova |
2018 |
Walters JLH, De Iuliis GN, Dun MD, Aitken RJ, McLaughlin EA, Nixon B, Bromfield EG, 'Pharmacological inhibition of arachidonate 15-lipoxygenase protects human spermatozoa against oxidative stress.', Biology of reproduction, 98 784-794 (2018) [C1]
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Nova |
2018 |
Mihalas BP, Bromfield EG, Sutherland JM, De Iuliis GN, McLaughlin EA, John Aitken R, Nixon B, 'Oxidative damage in naturally aged mouse oocytes is exacerbated by dysregulation of proteasomal activity', Journal of Biological Chemistry, 293 18944-18964 (2018) [C1]
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Nova |
2017 |
Zhou W, De Iuliis GN, Turner AP, Reid AT, Anderson AL, McCluskey A, et al., 'Developmental expression of the dynamin family of mechanoenzymes in the mouse epididymis', BIOLOGY OF REPRODUCTION, 96 159-173 (2017) [C1]
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Nova |
2017 |
Bromfield EG, Aitken RJ, McLaughlin EA, Nixon B, 'Proteolytic degradation of heat shock protein A2 occurs in response to oxidative stress in male germ cells of the mouse', MOLECULAR HUMAN REPRODUCTION, 23 91-105 (2017) [C1]
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Nova |
2017 |
Camlin NJ, Jarnicki AG, Vanders RL, Walters KA, Hansbro PM, McLaughlin EA, Holt JE, 'Grandmaternal smoke exposure reduces female fertility in a murine model, with great-grandmaternal smoke exposure unlikely to have an effect', HUMAN REPRODUCTION, 32 1270-1281 (2017) [C1]
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Nova |
2017 |
Sominsky L, Hodgson DM, McLaughlin EA, Smith R, Wall HM, Spencer SJ, 'Linking stress and infertility: a novel role for ghrelin', Endocrine Reviews, 38 432-467 (2017) [C1]
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Nova |
2017 |
Camlin NJ, McLaughlin EA, Holt JE, 'Motoring through: the role of kinesin superfamily proteins in female meiosis', HUMAN REPRODUCTION UPDATE, 23 409-420 (2017) [C1]
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Nova |
2017 |
Camlin NJ, McLaughlin EA, Holt JE, 'Kif4 Is Essential for Mouse Oocyte Meiosis', PLOS ONE, 12 (2017) [C1]
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Nova |
2017 |
Camlin NJ, McLaughlin EA, Holt JE, 'The use of C57Bl/6xCBA F1 hybrid cross as a model for human age-related oocyte aneuploidy', MOLECULAR REPRODUCTION AND DEVELOPMENT, 84 6-7 (2017)
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2017 |
Bromfield EG, Mihalas BP, Dun MD, Aitken RJ, McLaughlin EA, Walters JLH, Nixon B, 'Inhibition of arachidonate 15-lipoxygenase prevents 4-hydroxynonenal-induced proteindamage in male germ cells', Biology of Reproduction, 96 598-609 (2017) [C1]
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Nova |
2017 |
Trinh TN, McLaughlin EA, Gordon CP, Bernstein IR, Pye VJ, Redgrove KA, McCluskey A, 'Small molecule Hedgehog pathway antagonists', ORGANIC & BIOMOLECULAR CHEMISTRY, 15 3046-3059 (2017) [C1]
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Nova |
2017 |
Mihalas BP, De Iuliis GN, Redgrove KA, McLaughlin EA, Nixon B, 'The lipid peroxidation product 4-hydroxynonenal contributes to oxidative stress-mediated deterioration of the ageing oocyte', SCIENTIFIC REPORTS, 7 (2017) [C1]
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Nova |
2017 |
Mihalas BP, Redgrove KA, McLaughlin EA, Nixon B, 'Molecular mechanisms responsible for increased vulnerability of the ageing oocyte to oxidative damage', Oxidative Medicine and Cellular Longevity, 2017 1-22 (2017) [C1]
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Nova |
2017 |
Hutcheon K, McLaughlin EA, Stanger SJ, Bernstein IR, Dun MD, Eamens AL, Nixon B, 'Analysis of the small non-protein-coding RNA profile of mouse spermatozoa reveals specific enrichment of piRNAs within mature spermatozoa', RNA Biology, 14 1776-1790 (2017) [C1]
Post-testicular sperm maturation and storage within the epididymis is a key determinant of gamete quality and fertilization competence. Here we demonstrate that mouse spermatozoa ... [more]
Post-testicular sperm maturation and storage within the epididymis is a key determinant of gamete quality and fertilization competence. Here we demonstrate that mouse spermatozoa possess a complex small non-protein-coding RNA (sRNA) profile, the composition of which is markedly influenced by their epididymal transit. Thus, although microRNAs (miRNAs) are highly represented in the spermatozoa of the proximal epididymis, this sRNA class is largely diminished in mature spermatozoa of the distal epididymis. Coincident with this, a substantial enrichment in Piwi-interacting RNA (piRNA) abundance in cauda spermatozoa was detected. Further, features of cauda piRNAs, including; predominantly 29¿31 nts in length; preference for uracil at their 5' terminus; no adenine enrichment at piRNA nt 10, and; predominantly mapping to intergenic regions of the mouse genome, indicate that these piRNAs are generated by the PIWIL1-directed primary piRNA production pathway. Accordingly, PIWIL1 was detected via immunoblotting and mass spectrometry in epididymal spermatozoa. These data provide insight into the complexity and dynamic nature of the sRNA profile of spermatozoa and raise the intriguing prospect that piRNAs are generated in situ in maturing spermatozoa. Such information is of particular interest in view of the potential role for paternal sRNAs in influencing conception, embryo development and intergenerational inheritance.
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Nova |
2017 |
Zhou W, Anderson AL, Turner AP, De Iuliis GN, McCluskey A, McLaughlin EA, Nixon B, 'Characterization of a novel role for the dynamin mechanoenzymes in the regulation of human sperm acrosomal exocytosis.', Molecular Human Reproduction, 23 657-673 (2017) [C1]
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Nova |
2017 |
Fuller EA, Sominsky L, Sutherland JM, Redgrove KA, Harms L, McLaughlin EA, Hodgson DM, 'Neonatal immune activation depletes the ovarian follicle reserve and alters ovarian acute inflammatory mediators in neonatal rats', BIOLOGY OF REPRODUCTION, 97 719-730 (2017) [C1]
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Nova |
2016 |
Redgrove KA, Bernstein IR, Pye VJ, Mihalas BP, Sutherland JM, Nixon B, et al., 'Dynamin 2 is essential for mammalian spermatogenesis', SCIENTIFIC REPORTS, 6 (2016) [C1]
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Nova |
2016 |
Bromfield EG, McLaughlin EA, Aitken RJ, Nixon B, 'Heat shock protein member A2 forms a stable complex with angiotensin convertingenzymeand protein disulfide isomerase A6 in human spermatozoa', Molecular Human Reproduction, 22 93-109 (2016) [C1]
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Nova |
2016 |
Shapouri F, Saeidi S, de Iongh RU, Casagranda F, Western PS, McLaughlin EA, et al., 'Tob1 is expressed in developing and adult gonads and is associated with the P-body marker, Dcp2', Cell and Tissue Research, 364 443-451 (2016) [C1]
Tob1 is a member of the BTG/TOB family of proteins with established antiproliferative function. In Danio rerio and Xenopus laevis, the Tob1 gene is expressed from the one-cell sta... [more]
Tob1 is a member of the BTG/TOB family of proteins with established antiproliferative function. In Danio rerio and Xenopus laevis, the Tob1 gene is expressed from the one-cell stage through to early gastrula stages, followed in later development by discrete expression in many tissues including the notochord and somites. In both mouse and human, Tob1 is expressed in many adult tissues including the testis and ovary; however, the specific cell types are unknown. We examine Tob1 gene expression in mouse in developing germ cells and in sorted male germ cells (gonocytes, spermatogonia, pachytene spermatocytes and round spermatids) by reverse transcription and droplet digital polymerase chain reaction (RT-ddPCR) and in adult ovary and testis by immunofluorescence with anti-Tob1 protein staining. By RT-ddPCR, Tob1 expression was low in developing male germ cells but was highly expressed in round spermatids. In developing female germ cells undergoing entry into meiosis, it increased 10-fold. Tob1 was also highly expressed in round spermatids and in oocytes in all stages of folliculogenesis. Notably, a marker for P-bodies, Dcp-2, was also highly expressed in round spermatids and all oocyte stages examined. The cytoplasmic presence of Tob1 protein in round spermatids and oocytes and the association of Tob1 protein with Dcp2 in both cell types suggest that Tob1 protein plays a role in post-transcriptional mechanisms.
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Nova |
2016 |
Stanger SJ, Law EA, Jamsai D, O'Bryan MK, Nixon B, McLaughlin EA, et al., 'A novel germ cell protein, SPIF (sperm PKA interacting factor), is essential for the formation of a PKA/TCP11 complex that undergoes conformational and phosphorylation changes upon capacitation', FASEB Journal, 30 2777-2791 (2016) [C1]
Spermatozoa require the process of capacitation to enable them to fertilize an egg. PKA is crucial to capacitation and the development of hyperactivated motility. Sperm PKA is act... [more]
Spermatozoa require the process of capacitation to enable them to fertilize an egg. PKA is crucial to capacitation and the development of hyperactivated motility. Sperm PKA is activated by cAMP generated by the germ cell-enriched adenylyl cyclase encoded by Adcy10. Male mice lacking Adcy10 are sterile, because their spermatozoa are immotile. The current studywas designed to identify binding partners of the sperm-specific (Ca2) catalytic subunit of PKA (PRKACA) by using it as the "bait" in a yeast 2-hybrid system. This approach was used to identify a novel germ cell-enriched protein, sperm PKA interacting factor (SPIF), in 25% of the positive clones. Homozygous Spif-nullmice were embryonically lethal. SPIF was coexpressed and coregulated with PRKACA and with t-complex protein (TCP)-11, a protein associated with PKA signaling. We established that these 3 proteins form part of a novel complex in mouse spermatozoa. Upon capacitation, the SPIF protein becomes tyrosine phosphorylatedin > 95% of sperm. Anapparent molecular rearrangement in the complex occurs, bringing PRKACA and TCP11 into proximity. Taken together, these results suggest a role for the novel complex of SPIF, PRKACA, and TCP11 during spermcapacitation, fertilization, and embryogenesis.
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Nova |
2016 |
Reilly JN, McLaughlin EA, Stanger SJ, Anderson AL, Hutcheon K, Church K, et al., 'Characterisation of mouse epididymosomes reveals a complex profile of microRNAs and a potential mechanism for modification of the sperm epigenome', SCIENTIFIC REPORTS, 6 (2016) [C1]
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Nova |
2016 |
Camlin NJ, Sobinoff AP, Sutherland JM, Beckett EL, Jarnicki AG, Vanders RL, et al., 'Maternal Smoke Exposure Impairs the Long-Term Fertility of Female Offspring in a Murine Model.', Biol Reprod, 94 39 (2016) [C1]
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Nova |
2016 |
Trinh TN, McLaughlin EA, Abdel-Hamid MK, Gordon CP, Bernstein IR, Pye V, et al., 'Quinolone-1-(2H)-ones as hedgehog signalling pathway inhibitors', ORGANIC & BIOMOLECULAR CHEMISTRY, 14 6304-6315 (2016) [C1]
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Nova |
2016 |
Kumar M, Camlin NJ, Holt JE, Teixeira JM, McLaughlin EA, Tanwar PS, 'Germ cell specific overactivation of WNT/ßcatenin signalling has no effect on folliculogenesis but causes fertility defects due to abnormal foetal development', SCIENTIFIC REPORTS, 6 (2016) [C1]
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Nova |
2016 |
Fuller EA, Carey MC, Redgrove KA, McLaughlin EA, Hodgson DM, 'Abstract # 1761 Neonatal immune activation with lipopolysaccharide and a second hit of adulthood stress alters ovarian inflammatory mediators: Implications for female subfertility', Brain, Behavior, and Immunity, 57 e15-e16 (2016)
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2015 |
Sutherland JM, Sobinoff AP, Fraser BA, Redgrove KA, Davidson TL, Siddall NA, et al., 'RNA binding protein Musashi-1 directly targets Msi2 and Erh during early testis germ cell development and interacts with IPO5 upon translocation to the nucleus', FASEB Journal, 29 2759-2768 (2015) [C1]
Controlled gene regulation during gamete development is vital for maintaining reproductive potential. During the process of gamete development, male germ cells experience extended... [more]
Controlled gene regulation during gamete development is vital for maintaining reproductive potential. During the process of gamete development, male germ cells experience extended periods of inactive transcription despite requirements for continued growth and differentiation. Spermatogenesis therefore provides an ideal model to study the effects of posttranscriptional control on gene regulation. During spermatogenesis posttranscriptional regulation is orchestrated by abundantly expressed RNA-binding proteins. One such group of RNA-binding proteins is the Musashi family, previously identified as a critical regulator of testis germ cell development and meiosis in Drosophila and also shown to be vital to sperm development and reproductive potential in the mouse. We focus in depth on the role and function of the vertebrate Musashi ortholog Musashi-1 (MSI1). Through detailed expression studies and utilizing our novel transgenic Msi1 testis-specific overexpression model, we have identified 2 unique RNA-binding targets of MSI1 in spermatogonia,Msi2 and Erh, and have demonstrated a role for MSI1 in translational regulation. We have also provided evidence to suggest that nuclear import protein, IPO5, facilitates the nuclear translocation of MSI1 to the transcriptionally silenced XY chromatin domain in meiotic pachytene spermatocytes, resulting in the release of MSI1 RNA-binding targets. This firmly establishes MSI1 as a master regulator of posttranscriptional control during early spermatogenesis and highlights the significance of the subcellular localization of RNA binding proteins in relation to their function.
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Nova |
2015 |
Stenevi U, Lundström M, 'Can endophthalmitis be prevented?', Acta Ophthalmol, 93 301-302 (2015)
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2015 |
Mihalas BP, Western PS, Loveland KL, McLaughlin EA, Holt JE, 'Changing expression and subcellular distribution of karyopherins during murine oogenesis', REPRODUCTION, 150 485-496 (2015) [C1]
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Nova |
2015 |
Anderson AL, Stanger SJ, Mihalas BP, Tyagi S, Holt JE, McLaughlin EA, Nixon B, 'Assessment of microRNA expression in mouse epididymal epithelial cells and spermatozoa by next generation sequencing', Genomics Data, 6 208-211 (2015) [C1]
The mammalian epididymis is a highly specialized region of the male reproductive tract that is lined with a continuous layer of epithelial cells that display a remarkable level of... [more]
The mammalian epididymis is a highly specialized region of the male reproductive tract that is lined with a continuous layer of epithelial cells that display a remarkable level of regionalized secretory and absorptive activity. The luminal environment created by this combined secretory and absorptive activity is directly responsible for promoting the functional maturation of spermatozoa and their maintenance in a quiescent and viable state prior to ejaculation. This study was designed to identify the complement of microRNAs (miRNAs) that are expressed within the mouse epididymal epithelial cells and the maturing populations of spermatozoa. Through the use of Next Generation Sequencing technology we have demonstrated that both epididymal epithelial cells and spermatozoa harbour a complex repertoire of miRNAs that have substantially different expression profiles along the length of the tract. These data, deposited in the Gene Expression Omnibus (GEO) with the accession numbers GSE70197 and GSE70198, afford valuable insight into the post-transcriptional control of gene expression within the epididymis and provide the first evidence for the dynamic transformation of the miRNA content of maturing sperm cells. Ultimately such information promises to inform our understanding of the aetiology of male infertility. Herein we provide a detailed description of the methodology used to generate these important data.
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Nova |
2015 |
Sobinoff AP, Dando SJ, Redgrove KA, Sutherland JM, Stanger SJ, Armitage CW, et al., 'Chlamydia muridarum infection-induced destruction of male germ cells and sertoli cells is partially prevented by Chlamydia major outer membrane protein-specific immune CD4 cells.', Biol Reprod, 92 27 (2015) [C1]
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Nova |
2015 |
Nixon B, Stanger SJ, Mihalas BP, Reilly JN, Anderson AL, Dun MD, et al., 'Next generation sequencing analysis reveals segmental patterns of microRNA expression in mouse epididymal epithelial cells', PLoS ONE, 10 (2015) [C1]
The functional maturation of mammalian spermatozoa is accomplished as the cells descend through the highly specialized microenvironment of the epididymis. This dynamic environment... [more]
The functional maturation of mammalian spermatozoa is accomplished as the cells descend through the highly specialized microenvironment of the epididymis. This dynamic environment is, in turn, created by the combined secretory and absorptive activity of the surrounding epithelium and displays an extraordinary level of regionalization. Although the regulatory network responsible for spatial coordination of epididymal function remains unclear, recent evidence has highlighted a novel role for the RNA interference pathway. Indeed, as noncanonical regulators of gene expression, small noncoding RNAs have emerged as key elements of the circuitry involved in regulating epididymal function and hence sperm maturation. Herein we have employed next generation sequencing technology to profile the genome-wide miRNA signatures of mouse epididymal cells and characterize segmental patterns of expression. An impressive profile of some 370 miRNAs were detected in the mouse epididymis, with a subset of these specifically identified within the epithelial cells that line the tubule (218). A majority of the latter miRNAs (75%) were detected at equivalent levels along the entire length of the mouse epididymis. We did however identify a small cohort of miRNAs that displayed highly regionalized patterns of expression, including miR-204-5p and miR-196b-5p, which were down- and up-regulated by approximately 39- and 45-fold between the caput/caudal regions, respectively. In addition we identified 79 miRNAs (representing ~ 21% of all miRNAs) as displaying conserved expression within all regions of the mouse, rat and human epididymal tissue. These included 8/14 members of let-7 family of miRNAs that have been widely implicated in the control of androgen signaling and the repression of cell proliferation and oncogenic pathways. Overall these data provide novel insights into the sophistication of the miRNA network that regulates the function of the male reproductive tract.
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Nova |
2015 |
Finke EH, Hickerson B, McLaughlin E, 'Parental Intention to Support Video Game Play by Children With Autism Spectrum Disorder: An Application of the Theory of Planned Behavior', LANGUAGE SPEECH AND HEARING SERVICES IN SCHOOLS, 46 154-165 (2015)
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2015 |
Nixon B, Stanger SJ, Mihalas BP, Reilly JN, Anderson AL, Tyagi S, et al., 'The MicroRNA Signature of Mouse Spermatozoa Is Substantially Modified During Epididymal Maturation', Biology of Reproduction, 93 (2015) [C1]
In recent years considerable effort has been devoted to understanding the epigenetic control of sperm development, leading to an increased appreciation of the importance of RNA in... [more]
In recent years considerable effort has been devoted to understanding the epigenetic control of sperm development, leading to an increased appreciation of the importance of RNA interference pathways, and in particular miRNAs, as key regulators of spermatogenesis and epididymal maturation. It has also been shown that sperm are endowed with an impressive array of miRNA that have been implicated in various aspects of fertilization and embryo development. However, to date there have been no reports on whether the sperm miRNA signature is static or whether it is influenced by their prolonged maturation within the male reproductive tract. To investigate this phenomenon, we employed next-generation sequencing to systematically profile the miRNA signature of maturing mouse spermatozoa. In so doing we have provided the first evidence for the posttesticular modification of the sperm miRNA profile under normal physiological conditions. Such modifications include the apparent loss and acquisition of an impressive cohort of some 113 and 115 miRNAs, respectively, between the proximal and distal epididymal segments. Interestingly, the majority of these changes occur late in maturation and include the uptake of novel miRNA species in addition to a significant increase in many miRNAs natively expressed in immature sperm. Because sperm are not capable of de novo transcription, these findings identify the epididymis as an important site in establishing the sperm epigenome with the potential to influence the peri-conceptual environment of the female reproductive tract, contribute to the inheritance of acquired characteristics, and/or alter the developmental trajectory of the resulting offspring.
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Nova |
2015 |
Reid AT, Anderson AL, Roman SD, McLaughlin EA, McCluskey A, Robinson PJ, et al., 'Glycogen synthase kinase 3 regulates acrosomal exocytosis in mouse spermatozoa via dynamin phosphorylation', FASEB JOURNAL, 29 2872-2882 (2015) [C1]
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Nova |
2015 |
Bromfield EG, Aitken RJ, Anderson AL, McLaughlin EA, Nixon B, 'The impact of oxidative stress on chaperone-mediated human sperm-egg interaction.', Hum Reprod, 30 2597-2613 (2015) [C1]
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Nova |
2015 |
Nixon B, Bromfield EG, Dun MD, Redgrove KA, McLaughlin EA, Aitken RJ, 'The role of the molecular chaperone heat shock protein A2 (HSPA2) in regulating human sperm-egg recognition', ASIAN JOURNAL OF ANDROLOGY, 17 568-573 (2015) [C1]
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Nova |
2015 |
Sutherland JM, Sobinoff AP, Gunter KM, Fraser BA, Pye V, Bernstein IR, et al., 'Knockout of RNA Binding Protein MSI2 Impairs Follicle Development in the Mouse Ovary: Characterization of MSI1 and MSI2 during Folliculogenesis.', Biomolecules, 5 1228-1244 (2015) [C1]
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Nova |
2015 |
Fuller EA, Sominsky L, McLaughlin EA, Hodgson DM, 'Acute neonatal immune challenge: Lipopolysaccharide exposure alters neonatal ovarian inflammatory pathways and mediators', Brain, Behavior, and Immunity, 49 e35-e35 (2015)
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2014 |
Robker RL, Watson LN, Robertson SA, Dunning KR, McLaughlin EA, Russell DL, 'Identification of sites of STAT3 action in the female reproductive tract through conditional gene deletion', PLoS ONE, 9 (2014) [C1]
The STAT3 transcription factor is a pleiotropic transducer of signalling by hormones, growth factors and cytokines that has been identified in the female reproductive tract from o... [more]
The STAT3 transcription factor is a pleiotropic transducer of signalling by hormones, growth factors and cytokines that has been identified in the female reproductive tract from oocytes and granulosa cells of the ovary to uterine epithelial and stromal cells. In the present study we used transgenic models to investigate the importance of STAT3 for reproductive performance in these different tissues. The Cre-LoxP system was used to delete STAT3 in oocytes by crossing Stat3 fl/fl with Zp3-cre+ mice, or in ovarian granulosa cells and uterine stroma by crossing with Amhr2-Cre+ mice. Surprisingly, deletion of STAT3 in oocytes had no effect on fertility indicating that the abundance of STAT3 protein in maturing oocytes and fertilized zygotes is not essential to these developmental stages. In Stat3fl/fl;Amhr2-cre + females impaired fertility was observed through significantly fewer litters and smaller litter size. Ovulation rate, oocyte fertilization and development to blastocyst were unaffected in this line; however, poor recombination efficiency in granulosa cells had yielded no net change in STAT3 protein abundance. In contrast, uteri from these mice showed STAT3 protein depletion selectively from the stomal compartment. A significant reduction in number of viable fetuses on gestational day 18, increased fetal resorptions and disrupted placental morphology were evident causes of the reduced fertility. In conclusion, this study defines an important role for STAT3 in uterine stromal cells during embryo implantation and the development of a functional placenta. © 2014 Robker et al.
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Nova |
2014 |
Redgrove KA, McLaughlin EA, 'The role of the immune response in chlamydia trachomatis infection of the male genital tract: A double-edged sword', Frontiers in Immunology, 5 1-22 (2014) [C1]
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Nova |
2014 |
Camlin NJ, McLaughlin EA, Holt JE, 'Through the smoke: Use of in vivo and in vitro cigarette smoking models to elucidate its effect on female fertility', Toxicology and Applied Pharmacology, 281 266-275 (2014) [C1]
A finite number of oocytes are established within the mammalian ovary prior to birth to form a precious ovarian reserve. Damage to this limited pool of gametes by environmental fa... [more]
A finite number of oocytes are established within the mammalian ovary prior to birth to form a precious ovarian reserve. Damage to this limited pool of gametes by environmental factors such as cigarette smoke and its constituents therefore represents a significant risk to a woman's reproductive capacity. Although evidence from human studies to date implicates a detrimental effect of cigarette smoking on female fertility, these retrospective studies are limited and present conflicting results. In an effort to more clearly understand the effect of cigarette smoke, and its chemical constituents, on female fertility, a variety of in vivo and in vitro animal models have been developed. This article represents a systematic review of the literature regarding four of experimental model types: 1) direct exposure of ovarian cells and follicles to smoking constituents' in vitro, 2) direct exposure of whole ovarian tissue with smoking constituents in vitro, 3) whole body exposure of animals to smoking constituents and 4) whole body exposure of animals to cigarette smoke. We summarise key findings and highlight the strengths and weaknesses of each model system, and link these to the molecular mechanisms identified in smoke-induced fertility changes.
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Nova |
2014 |
Yun Y, Holt JE, Lane SIR, McLaughlin EA, Merriman JA, Jones KT, 'Reduced ability to recover from spindle disruption and loss of kinetochore spindle assembly checkpoint proteins in oocytes from aged mice', Cell Cycle, 13 1938-1947 (2014) [C1]
Currently, maternal aging in women, based on mouse models, is thought to raise oocyte aneuploidy rates, because chromosome cohesion deteriorates during prophase arrest, and Sgo2, ... [more]
Currently, maternal aging in women, based on mouse models, is thought to raise oocyte aneuploidy rates, because chromosome cohesion deteriorates during prophase arrest, and Sgo2, a protector of centromeric cohesion, is lost. Here we show that the most common mouse strain, C57Bl6/J, is resistant to maternal aging, showing little increase in aneuploidy or Sgo2 loss. Instead it demonstrates significant kinetochore-associated loss in the spindle assembly checkpoint protein Mad2 and phosphorylated Aurora C, which is involved in microtubule-kinetochore error correction. Their loss affects the fidelity of bivalent segregation but only when spindle organization is impaired during oocyte maturation. These findings have an impact clinically regarding the handling of human oocytes ex vivo during assisted reproductive techniques and suggest there is a genetic basis to aneuploidy susceptibility. © 2014 Landes Bioscience.
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Nova |
2014 |
Holt JE, Pye V, Boon E, Stewart JL, Garcia-Higuera I, Moreno S, et al., 'The APC/C activator FZR1 is essential for meiotic prophase I in mice', DEVELOPMENT, 141 1354-U327 (2014) [C1]
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Nova |
2014 |
Trinh TN, McLaughlin EA, Gordon CP, McCluskey A, 'Hedgehog signalling pathway inhibitors as cancer suppressing agents', MedChemComm, 5 117-133 (2014) [C1]
The Hedgehog (Hh) signalling pathway plays a pivotal role in the spatial and temporal regulation of cell proliferation and differentiation. By controlling the correct maturation o... [more]
The Hedgehog (Hh) signalling pathway plays a pivotal role in the spatial and temporal regulation of cell proliferation and differentiation. By controlling the correct maturation of developing tissues and ensuring attainment of the correct size, position and the presence of fully functioning cellular structures, the Hh plays a pivotal role in development. Conversely aberrant Hh signalling is involved in Gorlin syndrome, basal cell carcinoma (the most common cancer in the world), and more than one third of all human medulloblastoma cases. In all of these cases, it is believed that deregulated Hh signalling leads to increased cell proliferation and tumour formation. Inhibition of the Hedgehog signalling pathway, is a recently validated anti-cancer drug target, with vismodegib (Erivedge¿), approved by the U.S. Food and Drug Administration for the treatment of adult basal cell carcinoma. In this perspective we outline the current state of Hh pathway inhibitors with a particular focus on potential limitations of upstream Hh pathway inhibition in relation to resistance mutations and crosstalk pathways. Together, these limitations indicate that inhibition of downstream components, specifically the Gli family of transcription factors, may represent a next generation approach to suppress tumours associated with aberrant Hh pathway signalling. © 2014 The Royal Society of Chemistry.
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Nova |
2014 |
Sahin Z, Szczepny A, Mclaughlin EA, Meistrich ML, Zhou W, Ustunel I, Loveland KL, 'Dynamic Hedgehog signalling pathway activity in germline stem cells', Andrology, 2 267-274 (2014) [C1]
Summary: Although the contribution of Hedgehog (Hh) signalling to stem cell development and oncogenesis is well recognised, its importance for spermatogonial stem cells (SSCs) has... [more]
Summary: Although the contribution of Hedgehog (Hh) signalling to stem cell development and oncogenesis is well recognised, its importance for spermatogonial stem cells (SSCs) has not been established. Here we interrogate adult rat SSCs using an established model in which only undifferentiated spermatogonial cells remain in the testis at 15 weeks following irradiation, and spermatogonial differentiation is induced within 4 weeks by gonadotrophin-releasing hormone antagonist (GnRH-ant) administration. Synthesis of Hh pathway components in untreated adult rat testes was compared with that in irradiated testes prior to and after GnRH-ant exposure using in situ hybridization. In adult testes with complete spermatogenesis, the Desert Hedgehog ligand transcript, Dhh, was detected in Sertoli cells, some spermatogonia and in spermatocytes by in situ hybridization. Spermatogenic cells were identified as sites of Hh signalling through detection of transcripts encoding the Hh receptor, Ptc2 transcripts and proteins for the key downstream target of Hh signalling, Gli1 and the Hh transcriptional activator, Gli2. Remarkably, the undifferentiated spermatogonia present in irradiated adult rat testes contained Dhh in addition to Ptc2, Gli1 and Gli2, revealing the potential for an autocrine Hh signalling loop to sustain undifferentiated spermatogonial cells. These transcripts became undetectable by in situ hybridization following GnRH-ant induction of spermatogonial differentiation, however, detection of Gli1 protein in spermatogonia in all groups indicates that Hh signalling is sustained. This is the first evidence of active Hh signalling in mammalian male germline stem cells, as has been documented for some cancer stem cells. © 2014 American Society of Andrology and European Academy of Andrology.
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Nova |
2014 |
Sutherland JM, Fraser BA, Sobinoff AP, Pye VJ, Davidson T-L, Siddall NA, et al., 'Developmental expression of Musashi-1 and Musashi-2 RNA-binding proteins during spermatogenesis: analysis of the deleterious effects of dysregulated expression.', Biol Reprod, 90 92 (2014) [C1]
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Nova |
2014 |
Fuller EA, Sominsky L, Rolfe KT, Sutherland JM, McLaughlin EA, Hodgson DM, '93. Acute neonatal immune challenge: Implications for the programming of later life female sub-fertility', Brain, Behavior, and Immunity, 40 e27-e27 (2014)
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2014 |
Sobinoff AP, Sutherland JM, Beckett EL, Stanger SJ, Johnson R, Jarnicki AG, et al., 'Damaging legacy: maternal cigarette smoking has long-term consequences for male offspring fertility.', Hum Reprod, 29 2719-2735 (2014) [C1]
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Nova |
2013 |
McIver SC, Roman SD, Nixon B, Loveland KL, McLaughlin EA, 'The rise of testicular germ cell tumours: The search for causes, risk factors and novel therapeutic targets', F1000 Research, 2 1-11 (2013) [C1]
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Nova |
2013 |
McIver SC, Roman SD, Nixon B, Loveland KL, McLaughlin EA, 'The rise of testicular germ cell tumours: the search for causes, risk factors and novel therapeutic targets', F1000Research,
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2013 |
Merriman JA, Lane SIR, Holt JE, Jennings PC, García-Higuera I, Moreno S, et al., 'Reduced Chromosome Cohesion Measured by Interkinetochore Distance Is Associated with Aneuploidy Even in Oocytes from Young Mice1', Biology of Reproduction, 88 (2013) [C1]
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Nova |
2013 |
Sobinoff AP, Beckett EL, Jarnicki AG, Sutherland JM, McCluskey A, Hansbro PM, McLaughlin EA, 'Scrambled and fried: Cigarette smoke exposure causes antral follicle destruction and oocyte dysfunction through oxidative stress', TOXICOLOGY AND APPLIED PHARMACOLOGY, 271 156-167 (2013) [C1]
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2013 |
Davies RG, Wagstaff KM, McLaughlin EA, Loveland KL, Jans DA, 'The BRCA1-binding protein BRAP2 can act as a cytoplasmic retention factor for nuclear and nuclear envelope-localizing testicular proteins', Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1833 3436-3444 (2013) [C1]
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Nova |
2013 |
Redgrove KA, Anderson AL, McLaughlin EA, O'Bryan MK, Aitken RJ, Nixon B, 'Investigation of the mechanisms by which the molecular chaperone HSPA2 regulates the expression of sperm surface receptors involved in human spermoocyte recognition', MOLECULAR HUMAN REPRODUCTION, 19 120-135 (2013) [C1]
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Nova |
2013 |
Sobinoff AP, Sutherland JM, Mclaughlin EA, 'Intracellular signalling during female gametogenesis', Molecular Human Reproduction, 19 265-278 (2013) [C1]
Female reproductive potential is dictated by the size of the primordial follicle pool and the correct regulation of oocyte maturation and activation-events essential for productio... [more]
Female reproductive potential is dictated by the size of the primordial follicle pool and the correct regulation of oocyte maturation and activation-events essential for production of viable offspring. Although a substantial body of work underpins our understanding of these processes, the molecular mechanisms of follicular and oocyte development are not fully understood. This review summarizes recent findings which have improved our conception of how folliculogenesis and oocyte competence are regulated, and discusses their implications for assisted reproductive techniques. We highlight evidence provided by genetically modified mouse models and in vitro studies which have refined our understanding of Pi3k/Akt and mTOR signalling in the oocyte and have discovered a role for Jak/Stat/Socs signalling in granulosa cells during primordial follicle activation. We also appraise a novel role for the metal ion zinc in the regulation of meiosis I and meiosis II progression through early meiosis inhibitor (Emi2) and Mos-Mapk signalling, and examine studies which expand our understanding of intracellular calcium signalling and extrinsic Plc¿ in stimulating oocyte activation. © The Author 2012. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved.
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Nova |
2013 |
O'Bryan MK, Clark BJ, McLaughlin EA, D'Sylva RJ, O'Donnell L, Wilce JA, et al., 'RBM5 Is a Male Germ Cell Splicing Factor and Is Required for Spermatid Differentiation and Male Fertility', PLOS GENETICS, 9 (2013) [C1]
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Nova |
2013 |
Sominsky L, Sobinoff AP, Jobling MS, Pye V, McLaughlin EA, Hodgson DM, 'Immune regulation of ovarian development: programming by neonatal immune challenge.', Frontiers in Neuroscience, 7 100 (2013) [C1]
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Nova |
2013 |
Sominsky L, Sobinoff AP, Pye V, McLaughlin EA, Hodgson DM, '10. Modulation of inflammatory signalling pathways in the ovary by immune challenge in early life', Brain, Behavior, and Immunity, 32 e3-e3 (2013)
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2013 |
McIver SC, Loveland KL, Roman SD, Nixon B, Kitazawa R, McLaughlin EA, 'The chemokine CXCL12 and its receptor CXCR4 are implicated in human seminoma metastasis', ANDROLOGY, 1 517-529 (2013) [C1]
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Nova |
2012 |
Merriman-Jones JA, Jennings PC, McLaughlin EA, Jones KT, 'Effect of aging on superovulation efficiency, aneuploidy rates, and sister chromatid cohesion in mice aged up to 15 months', Biology of Reproduction, 86 1-6 (2012) [C1]
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Nova |
2012 |
Sominsky Bar L, Meehan CL, Walker AK, Bobrovskaya L, McLaughlin EA, Hodgson DM, 'Neonatal immune challenge alters reproductive development in the female rat', Hormones and Behavior, 62 345-355 (2012) [C1]
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Nova |
2012 |
Sutherland JM, Keightley RA, Nixon B, Roman SD, Robker RL, Russell DL, McLaughlin EA, 'Suppressor of cytokine signaling 4 (SOCS4): Moderator of ovarian primordial follicle activation', Journal of Cellular Physiology, 227 1188-1198 (2012) [C1]
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Nova |
2012 |
Sobinoff AP, Pye VJ, Nixon B, Roman SD, McLaughlin EA, 'Jumping the gun: Smoking constituent BaP causes premature primordial follicle activation and impairs oocyte fusibility through oxidative stress', Toxicology and Applied Pharmacology, 260 70-80 (2012) [C1]
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Nova |
2012 |
Dun MD, Anderson AL, Bromfield EG, Asquith KL, Emmett BJ, McLaughlin EA, et al., 'Investigation of the expression and functional significance of the novel mouse sperm protein, a disintegrin and metalloprotease with thrombospondin type 1 motifs number 10 (ADAMTS10)', International Journal of Andrology, 35 572-589 (2012) [C1]
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Nova |
2012 |
Sobinoff AP, Nixon B, Roman SD, McLaughlin EA, 'Staying alive: PI3K pathway promotes primordial follicle activation and survival in response to 3MC-induced ovotoxicity', Toxicological Sciences, 128 258-271 (2012) [C1]
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Nova |
2012 |
McIver SC, Roman SD, Nixon B, McLaughlin EA, 'miRNA and mammalian male germ cells', Human Reproduction Update, 18 44-59 (2012) [C1]
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Nova |
2012 |
McIver SC, Stanger SJ, Santarelli DMF, Roman SD, Nixon B, McLaughlin EA, 'A unique combination of male germ cell mirnas coordinates gonocyte differentiation', PLoS One, 7 1-14 (2012) [C1]
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Nova |
2012 |
Siddall NA, Kalcina M, Johanson TM, Monk AC, Casagranda F, Been RP, et al., 'Drosophila Rbp6 Is an orthologue of vertebrate Msi-1 and Msi-2, but does not function redundantly with dMsi to regulate germline stem cell behaviour', PloS One, 7 (2012) [C1]
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Nova |
2012 |
Sominsky L, Meehan CL, Walker AK, Bobrovskaya L, McLaughlin EA, Hodgson DM, '33. Programming of reproductive development by neonatal immunological challenge: Evidence for transgenerational inheritance', Brain, Behavior, and Immunity, 26 S9-S10 (2012)
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2011 |
Gunter KM, McLaughlin EA, 'Translational control in germ cell development: A role for the RNA-binding proteins Musashi-1 and Musashi-2', IUBMB Life, 63 678-685 (2011) [C1]
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Nova |
2011 |
Monk AC, Siddall NA, Fraser BA, McLaughlin EA, Hime GR, 'Differential roles of HOW in male and female Drosophila germline differentiation', PLoS ONE, 6 (2011) [C1]
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Nova |
2011 |
Redgrove KA, Anderson AL, Dun MD, McLaughlin EA, O'Bryan MK, Aitken RJ, Nixon B, 'Involvement of multimeric protein complexes in mediating the capacitation-dependent binding of human spermatozoa to homologous zonae pellucidae', Developmental Biology, 356 460-474 (2011) [C1]
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Nova |
2011 |
Nixon B, Mitchell LA, Anderson AL, McLaughlin EA, O'Bryan MK, Aitken RJ, 'Proteomic and functional analysis of human sperm detergent resistant membranes', Journal of Cellular Physiology, 226 2651-2665 (2011) [C1]
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Nova |
2011 |
McLaughlin EA, Aitken RJ, 'Is there a role for immunocontraception?', Molecular and Cellular Endocrinology, 335 78-88 (2011) [C1]
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Nova |
2011 |
Walker AK, Hiles SA, Sominsky Bar L, McLaughlin EA, Hodgson DM, 'Neonatal lipopolysaccharide exposure impairs sexual development and reproductive success in the Wistar rat', Brain, Behavior, and Immunity, 25 674-684 (2011) [C1]
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Nova |
2011 |
Sobinoff AP, Mahony M, Nixon B, Roman SD, McLaughlin EA, 'Understanding the villain: DMBA-induced preantral ovotoxicity involves selective follicular destruction and primordial follicle activation through PI3K/Akt and mTOR signaling', Toxicological Sciences, 123 563-575 (2011) [C1]
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Nova |
2010 |
Gunter KM, Fraser BA, Sobinoff AP, Pye V, Siddall NA, Hime GR, McLaughlin EA, '149. TRANSLATIONAL CONTROL IN FOLLICULOGENESIS AND OOCYTE DEVELOPMENT: A ROLE FOR RNA-BINDING PROTEIN MUSASHI-1', Reproduction, Fertility and Development, 22 67-67 (2010)
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2010 |
McLaughlin EA, Fraser BA, Pye V, Bigland M, Siddall NA, Hime GR, '167. MUSASHI FAMILY OF RNA BINDING PROTEINS: CELL CYCLE REGULATORS IN SPERMATOGENESIS', Reproduction, Fertility and Development, 22 85-85 (2010)
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2010 |
Hopkins L, Pye V, Fraser B, Holt J, Jones K, McLaughlin E, '320. THE ROLE OF FIZZY RELATED 1 IN MALE MEIOSIS', Reproduction, Fertility and Development, 22 120-120 (2010)
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2010 |
Monk AC, Siddall NA, Volk T, Fraser BA, Quinn LM, McLaughlin EA, Hime GR, 'HOW is required for stem cell maintenance in the drosophila testis and for the onset of transit-amplifying divisions', Cell Stem Cell, 6 348-360 (2010) [C1]
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Nova |
2010 |
Sobinoff AP, Pye VJ, Nixon B, Roman SD, McLaughlin EA, 'Adding insult to injury: Effects of xenobiotic-induced preantral ovotoxicity on ovarian development and oocyte fusibility', Toxicological Sciences, 118 653-666 (2010) [C1]
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Nova |
2010 |
Redgrove KA, Nixon B, McLaughlin EA, O'Bryan MK, Aitken RJ, '119. IDENTIFICATION AND CHARACTERISATION OF SURFACE PROTEIN COMPLEXES IN HUMAN SPERMATOZOA', Reproduction, Fertility and Development, 22 37-37 (2010)
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2010 |
Sobinoff AP, Pye V, Nixon B, Roman SD, McLaughlin EA, '121. SHORT TERM XENOBIOTIC EXPOSURE COMPROMISES LONG TERM OOCYTE VIABILITY', Reproduction, Fertility and Development, 22 39-39 (2010)
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2010 |
Sutherland JM, Keightley R, Robker RL, Russell DL, McLaughlin EA, '126. JAK/STAT SIGNALLING IN FOLLICULOGENESIS', Reproduction, Fertility and Development, 22 44-44 (2010)
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2010 |
Watson LN, Robker RL, Dunning KR, McLaughlin EA, Russell DL, '130. CONDITIONAL TARGETED DELETIONS OF STAT3 TO IDENTIFY ITS ROLE IN OOCYTESAND GRANULOSA CELLS', Reproduction, Fertility and Development, 22 48-48 (2010)
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2010 |
McIver SC, Roman SD, Nixon B, McLaughlin EA, '142. microRNA AND EARLY MALE GERM CELLS', Reproduction, Fertility and Development, 22 60-60 (2010)
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2010 |
Anderson AL, Mitchell L, McLaughlin EA, O'Bryan MK, Aitken RJ, Nixon B, '322. PROTEOMIC AND FUNCTIONAL ANALYSIS OF HUMAN SPERM DETERGENT RESISTANT MEMBRANES', Reproduction, Fertility and Development, 22 122-122 (2010)
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2010 |
Bernstein IR, McLaughlin EA, O'Bryan MK, '324. SLX4, A KEY REGULATOR OF MEIOSIS AND DNA REPAIR IN THE MALE GERMLINE', Reproduction, Fertility and Development, 22 124-124 (2010)
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2010 |
Tannock SJ, McLaughlin EA, Aitken RJ, Roman SD, '326. SPIF - A NOVEL TESTIS-SPECIFIC GENE AND ITS INTERACTION WITH PKA', Reproduction, Fertility and Development, 22 126-126 (2010)
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2009 |
Gunter KM, Fraser BA, Sobinoff AP, Siddall NA, Hime GR, McLaughlin EA, '144. ROLE OF RNA-BINDING PROTEIN, MUSASHI-1 (Msi-1), IN MURINE FOLLICULOGENESIS AND OOCYTE DEVELOPMENT', Reproduction, Fertility and Development, 21 62-62 (2009)
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2009 |
Nixon B, Bielanowicz AJ, McLaughlin EA, Tanphaichitr N, Ensslin MA, Aitken RJ, 'Composition and significance of detergent resistant membranes in mouse spermatozoa', Journal of Cellular Physiology, 218 122-134 (2009) [C1]
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Nova |
2009 |
Yu DMT, Ajami K, Gall MG, Park J, Lee CS, Evans KA, et al., 'The in vivo expression of dipeptidyl peptidases 8 and 9', Journal of Histochemistry & Cytochemistry, 57 1025-1038 (2009) [C1]
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Nova |
2009 |
McLaughlin EA, McIver SC, 'Awakening the oocyte: Controlling primordial follicle development', Reproduction, 137 1-11 (2009) [C1]
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Nova |
2009 |
Sobinoff AP, Pye V, Nixon B, Roman SD, McLaughlin EA, '153. XENOBIOTICS; INFLUENCE ON OVARIAN FOLLICULAR DEVELOPMENT', Reproduction, Fertility and Development, 21 71-71 (2009)
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2009 |
Redgrove KA, McLaughlin EA, O'Bryan MK, Aitken RJ, Nixon B, '168. IDENTIFICATION AND CHARACTERISATION OF SURFACE PROTEIN COMPLEXES IN HUMAN SPERMATOZOA', Reproduction, Fertility and Development, 21 86-86 (2009)
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2009 |
Keightley RA, Nixon B, Roman SD, Russell DL, Robker RL, McLaughlin EA, '509. REGULATING MURINE FOLLICLE DEVELOPMENT BY STIMULATION OF THE JAK2/STAT3 SIGNAL TRANSDUCTION PATHWAY', Reproduction, Fertility and Development, 21 108-108 (2009)
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2008 |
McLaughlin EA, Gunter K, Woollett K, Roman SD, 'Chemokines: Role in Germ Cell Migration and Survival.', Biology of Reproduction, 78 53-53 (2008)
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2008 |
Fraser BA, Sobinoff A, Hime G, Siddall N, Roman S, McLaughlin EA, 'The Role of the RNA-Binding Protein, Musashi-1, in Murine Spermatogonial Stem Cell Maintenance.', Biology of Reproduction, 78 228-229 (2008)
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2008 |
Koppers AJ, De Iuliis GN, Read JM, McLaughlin EA, Aitken RJ, 'Significance of mitochondrial reactive oxygen species in the generation of oxidative stress in spermatozoa', Journal of Clinical Endocrinology & Metabolism, 93 3199-3207 (2008) [C1]
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Nova |
2008 |
Keightley R, McLaughlin E, Roman SD, Robker RL, Russell DL, '268. Suppressors of cytokine signalling (SOCS): roles in ovarian follicle activation', Reproduction, Fertility and Development, 20 68-68 (2008)
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2007 |
Fitzsimmons C, McLaughlin EA, Mahony MJ, Clulow J, 'Optimisation of handling, activation and assessment procedures for Bufo marinus spermatozoa', Reproduction, Fertility and Development, 19 594-601 (2007) [C1]
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2007 |
Aitken RJ, Wingate JK, De Iuliis GN, McLaughlin EA, 'Analysis of lipid peroxidation in human spermatozoa using BODIPY C11', Molecular Human Reproduction, 13 203-211 (2007) [C1]
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2007 |
Nixon B, Aitken RJ, McLaughlin EA, 'New insights into the molecular mechanisms of sperm-egg interaction', Cellular and Molecular Life Sciences, 64 1805-1823 (2007) [C1]
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2007 |
Aitken RJ, McLaughlin EA, 'Molecular mechanisms of sperm capacitation: progesterone-induced secondary calcium oscillations reflect the attainment of a capacitated state', Society of Reproduction and Fertility Supplement, 63 273-293 (2007) [C1]
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2006 |
De Iuliis GN, Wingate JK, Koppers AJ, McLaughlin EA, Aitken RJ, 'Definitive evidence for the nonmitochondrial production of superoxide anion by human spermatozoa', Journal of Clinical Endocrinology and Metabolism, 91 1968-1975 (2006) [C1]
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Nova |
2006 |
Aitken RJ, Wingate JK, De Iuliis GN, Koppers AJ, McLaughlin EA, 'Cis-unsaturated Fatty acids stimulate reactive oxygen species generation and lipid peroxidation in human spermatozoa', Journal of Clinical Endocrinology and Metabolism, 91 4154-4163 (2006) [C1]
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Nova |
2006 |
Holt JE, Roman SD, Aitken RJ, McLaughlin EA, 'Identification and characterization of a novel Mt-retrotransposon highly represented in the female mouse germline', Genomics, 87 490-499 (2006) [C1]
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2006 |
Curry BJ, Holt JE, McLaughlin EA, Aitken RJ, 'Characterization of structure and expression of the Dzip1 gene in the rat and mouse', Genomics, 87 275-285 (2006) [C1]
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2006 |
Holt JE, Jackson A, Roman SD, Aitken RJ, Koopman PA, McLaughlin EA, 'CXCR4/SDF1 interaction inhibits the primordial to primary follicle transition in the neonatal mouse ovary', Developmental Biology, 293 449-460 (2006) [C1]
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Nova |
2006 |
Hutt KJ, McLaughlin EA, Holland MK, 'Primordial follicle activation and follicular development in the juvenile rabbit ovary', Cell and Tissue Research, 326 809-822 (2006) [C1]
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2006 |
Hutt KJ, McLaughlin EA, Holland MK, 'Kit ligand and c-Kit have diverse roles during mammalian oogenesis and folliculogenesis', Molecular Human Reproduction, 12 61-69 (2006) [C1]
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2006 |
Prabhu SM, Meistrich ML, McLaughlin EA, Roman SD, Warne S, Mendis S, et al., 'Expression of c-Kit receptor mRNA and protein in the developing, adult and irradiated rodent testis', Reproduction, 131 489-499 (2006) [C1]
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2006 |
Siddall NA, McLaughlin EA, Marriner NL, Hime GR, 'The RNA-binding protein Musashi is required intrinsically to maintain stem cell identity', Proceedings of the National Academy of Sciences of the United States of America, 103 8402-8407 (2006) [C1]
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Nova |
2006 |
Mackenzie SM, McLaughlin EA, Perkins HD, French N, Sutherland T, Jackson RJ, et al., 'Immunocontraceptive effects on female rabbits infected with recombinant myxoma virus expressing rabbit ZP2 or ZP3', Biology of Reproduction, 74 511-521 (2006) [C1]
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2006 |
Hutt KJ, McLaughlin EA, Holland MK, 'KIT/KIT ligand in mammalian oogenesis and folliculogenesis: Roles in rabbit and murine ovarian follicle activation and oocyte growth', Biology of Reproduction, 75 421-433 (2006) [C1]
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2005 |
Asquith KL, Bielanowicz AJ, McLaughlin EA, Nixon B, Aitken RJ, 'Localization and significance of molecular chaperones, heat shock protein 1, and tumor rejection antigen gp96 in the male reproductive tract and during capacitation and acrosome reaction', Biology of Reproduction, 72 328-337 (2005) [C1]
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Nova |
2005 |
Telfer EE, Gosden RG, Byskov AG, Spears N, Albertini D, Andersen CY, et al., 'On regenerating the ovary and generating controversy', Cell, 122 821-822 (2005) [C1]
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2005 |
Prabhu SM, Meistrich ML, Mendis S, McLaughlin EA, Loveland KL, '216. c-kit Expression study: timing of onset in rodent testis and irradiated rat testis model', Reproduction, Fertility and Development, 17 83-83 (2005)
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2004 |
Blackmore DG, Baillie LR, Holt JE, Dierkx LM, Aitken RJ, McLaughlin EA, 'Biosynthesis of the Canine Zona Pellucida Requires the Integrated Participation of Both Oocytes and Granulosa Cells', Biology of Reproduction, 71 661-668 (2004) [C1]
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2004 |
Baker MA, Krutskikh A, Curry BJ, McLaughlin EA, Aitken RJ, 'Identification of Cytochrome P450-Reductase as the Enzyme Responsible for NADPH-Dependent Lucigenin and Tetrazolium Salt Reduction in Rat Epididymal Sperm Preparations', Biology of Reproduction, 71 307-318 (2004) [C1]
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2004 |
Asquith KL, Baleato R, McLaughlin EA, Nixon B, Aitken RJ, 'Tyrosine phosphorylation activates surface chaperones facilitating sperm-zona recognition', Journal of Cell Science, 117 3645-3657 (2004) [C1]
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Nova |
2004 |
Kerr PJ, Perkins HD, Inglis B, Stagg R, McLaughlin EA, Collins SV, Van Leeuwen BH, 'Expression of rabbit IL-4 by recombinant myxoma viruses enhances virulence and overcomes genetic resistance to myxomatosis', Virology, 324 117-128 (2004) [C1]
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2004 |
Curry BJ, Su H, Law EG, McLaughlin EA, Nixon B, Aitken RJ, 'Identification of RARhoGAP, a novel putative RhoGAP gene expressed in male germ cells', Genomics, 84 406-418 (2004) [C1]
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2004 |
Aitken RJ, Ryan AL, Baker MA, McLaughlin EA, 'Redox activity associated with the maturation and capacitation of mammalian spermatozoa', Free Radical Biology and Medicine, 36 994-1010 (2004) [C1]
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2004 |
Paul JW, McLaughlin EA, Aitken RJ, '207.Oolemmal proteomics: identification of oocyte cell surface protein complexes involved in sperm - egg interaction', Reproduction, Fertility and Development, 16 207-207 (2004)
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2004 |
Holt JE, Aitken RJ, Roman SS, McLaughlin EA, '217.Expression of the chemokine CXCL12 and its receptor CXCR4 in the activating mammalian ovarian follicle', Reproduction, Fertility and Development, 16 217-217 (2004)
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2003 |
McLaughlin EA, Holland MK, Aitken RJ, 'Contraceptive vaccines', Expert Opinion on Biological Therapy, 3 829-841 (2003) [C1]
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2003 |
Asquith KL, McLaughlin EA, Nixon B, Aitken RJ, '65. Localisation of tyrosine phosphorylated proteins on mouse spermatozoa during zona pellucida interaction and characterisation of sperm surface phosphoproteins', Reproduction, Fertility and Development, 15 65-65 (2003)
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2003 |
Hutt KJ, McLaughlin EA, Holland MK, '89. The effect of kit ligand on follicle growth initiation in cultured rabbit and mouse ovaries', Reproduction, Fertility and Development, 15 89-89 (2003)
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2002 |
Hancock P, McLaughlin EA, 'British Andrology Society guidelines for the assessment of post vasectomy semen samples', Journal of Clinical Pathology, 55 812-816 (2002) [C1]
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2002 |
McLaughlin EA, 'Cryopreservation, screening and storage of sperm The challenges for the twenty-first century', Human Fertility, 5 S61-S65 (2002) [C1]
The advent of HIV and the serious nature of the sequelae resulted in a major reassessment of artificial insemination practices in the UK. The development of human semen cryopreser... [more]
The advent of HIV and the serious nature of the sequelae resulted in a major reassessment of artificial insemination practices in the UK. The development of human semen cryopreservation had enormous impact on reproductive medicine and the availability of cryopreserved quarantined donor semen became a mainstay for the treatment of male infertility in the UK. The regulation and accreditation of assisted reproductive technologies and the introduction of peer-reviewed guidelines have largely standardized clinical and laboratory practice. The introduction of assisted fertilization techniques such as intracytoplasmic sperm injection, testicular sperm retrieval and improved oncology treatments have placed pressure on reproductive biologists and cryobiologists to design and use cryopreservation protocols for the optimum survival of sperm. © 2002, Informa UK Ltd All rights reserved: reproduction in whole or part not permitted. All rights reserved.
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2002 |
McLaughlin EA, 'Cryopreservation, screening and storage of sperm The challenges for the twenty-first century', Human Fertility, 5 S61-S65 (2002)
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2001 |
Ford WCL, Williams KM, Harrison S, Rees JM, Ray BD, McLaughlin EA, Hull MGR, 'Value of the hamster oocyte test and computerised measurements of sperm motility in predicting if four or more viable embryos will be obtained in an IVF cycle', INTERNATIONAL JOURNAL OF ANDROLOGY, 24 109-119 (2001) [C1]
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2001 |
McLaughlin EA, Frayne J, Bloomberg G, Hall L, 'Do fertilin beta and cyritestin play a major role in mammalian sperm-oolemma interactions? A critical re-evaluation of the use of peptide mimics in identifying specific oocyte recognition proteins', MOLECULAR HUMAN REPRODUCTION, 7 313-317 (2001)
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2001 |
McLaughlin E, 'The screeing of sperm donors - in search of the perfect male', ART & Science, (2001) [C3] |
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2000 |
McLaughlin EA, 'Recruitment of only cytomegalovirus (CMV) negative semen donors', HUMAN REPRODUCTION, 15 2248-2249 (2000) |
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2000 |
McLaughlin EA, 'Recruitment of only cytomegalovirus (CMV) negative semen donors', HUMAN REPRODUCTION, 15 2247-2247 (2000)
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2000 |
Curson R, Karakosta C, McLaughlin EA, 'Recruitment of only cytomegalovirus (CMV) negative semen donors (multiple letters)', Human Reproduction, 15 2247-2249 (2000)
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2000 |
Curson R, Karakosta C, 'Recruitment of only cytomegalovirus (CMV) negative semen donors', HUMAN REPRODUCTION, 15 2247-2248 (2000)
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2000 |
Turner K, Troup S, Morroll D, Glew A, McLaughlin E, Avery S, et al., 'Accreditation standards and guidelines for IVF laboratories', Human Fertility, 3 174-180 (2000)
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2000 |
McLaughlin DEA, '', Human Reproduction, 15 2248-2249 (2000)
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2000 |
McLaughlin DEA, 'Letters to the Editor', Human Reproduction, 15 2247-2247 (2000)
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1999 |
Whittington K, Harrison SC, Williams KM, Day JL, Mclaughlin EA, Hull MGR, Ford WCL, 'Reactive oxygen species (ROS) production and the outcome of diagnostic tests of sperm function', INTERNATIONAL JOURNAL OF ANDROLOGY, 22 236-242 (1999)
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1999 |
McLaughlin EA, 'Ensuring the safe cryopreservation of tissues & cells', CRYO-LETTERS, 20 133-136 (1999)
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1998 |
McLaughlin EA, Day J, Harrison S, Mitchell J, Prosser C, Hull M, 'Recruitment of gamete donors and payment of expenses', HUMAN REPRODUCTION, 13 1130-1132 (1998)
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1998 |
Hull MGR, Williams JAC, Ray B, McLaughlin EA, Akande VA, Ford WCL, 'The contribution of subtle oocyte or sperm dysfunction affecting fertilization in endometriosis-associated or unexplained infertility: A controlled comparison with tubal infertility and use of donor spermatozoa', HUMAN REPRODUCTION, 13 1825-1830 (1998)
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1998 |
Williams K, Frayne J, McLaughlin EA, Hall L, 'Expression of extracellular superoxide dismutase in the human male reproductive tract, detected using antisera raised against a recombinant protein', MOLECULAR HUMAN REPRODUCTION, 4 235-242 (1998)
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1997 |
Williams JAC, Ray B, McLaughlin EA, Hull MGR, 'The contribution of subtle oocyte or sperm dysfunction to endometriosis-associated and unexplained infertility', HUMAN REPRODUCTION, 12 P157-P157 (1997) |
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1997 |
Williams JAC, Ray B, McLaughlin EA, Hull MGR, 'P-157. The contribution of subtle oocyte or sperm dysfunction to endometriosis-associated and unexplained infertility', Human Reproduction, 12 194-194 (1997)
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1996 |
Coulson C, McLaughlin EA, Harris S, Ford WCL, Hull MGR, 'Randomized controlled trial of cervical cap with intracervical reservoir versus standard intracervical injection to inseminate cryopreserved donor semen', HUMAN REPRODUCTION, 11 84-87 (1996)
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1996 |
Liversedge NH, Jenkins JM, Keay SD, McLaughlin EA, AlSufyan H, Maile LA, et al., 'Antibiotic treatment based on seminal cultures from asymptomatic male partners in in-vitro fertilization is unnecessary and may be detrimental', HUMAN REPRODUCTION, 11 1227-1231 (1996)
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1996 |
Ford WCL, Williams KM, McLaughlin EA, Harrison S, Ray B, Hull MGR, 'The indirect immunobead test for seminal antisperm antibodies and fertilization rates at in-vitro fertilization', HUMAN REPRODUCTION, 11 1418-1422 (1996)
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1995 |
Plant A, McLaughlin EA, Ford WCL, 'Intracellular calcium measurements in individual human sperm demonstrate that the majority can respond to progesterone', Fertility and Sterility, 64 1213-1215 (1995)
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1995 |
PLANT A, MCLAUGHLIN EA, FORD WCL, 'INTRACELLULAR CALCIUM MEASUREMENTS IN INDIVIDUAL HUMAN SPERM DEMONSTRATE THAT THE MAJORITY CAN RESPOND TO PROGESTERONE', FERTILITY AND STERILITY, 64 1213-1215 (1995)
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1995 |
FOWLER PA, FAHY U, CULLER MD, KNIGHT PG, WARDLE PG, MCLAUGHLIN EA, et al., 'GONADOTROPIN SURGE-ATTENUATING FACTOR BIOACTIVITY IS PRESENT IN FOLLICULAR-FLUID FROM NATURALLY CYCLING WOMEN', HUMAN REPRODUCTION, 10 68-74 (1995)
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1994 |
FOWLER PA, FRASER M, CUNNINGHAM P, KNIGHT PG, BYRNE B, MCLAUGHLIN EA, et al., 'HIGHER GONADOTROPIN SURGE-ATTENUATING FACTOR BIOACTIVITY IS FOUND IN SMALL FOLLICLES FROM SUPEROVULATED WOMEN', JOURNAL OF ENDOCRINOLOGY, 143 33-44 (1994)
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1994 |
MCLAUGHLIN EA, FORD WCL, HULL MGR, 'ADENOSINE-TRIPHOSPHATE AND MOTILITY CHARACTERISTICS AF FRESH AND CRYOPRESERVED HUMAN SPERMATOZOA', INTERNATIONAL JOURNAL OF ANDROLOGY, 17 19-23 (1994)
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1994 |
FORD WCL, REES JM, McLAUGHLIN EA, LING L, HULL MGR, 'Pentoxifylline acts synergistically with A23187 to increase the penetration of zona-free hamster oocytes by cryopreserved human spermatozoa', International Journal of Andrology, 17 199-204 (1994)
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1994 |
CORRIGAN E, MCLAUGHLIN EA, COULSON N, FORD WCL, HULL MGR, 'THE EFFECT OF HALVING THE STANDARD-DOSE OF CRYOPRESERVED SEMEN FOR DONOR INSEMINATION - A CONTROLLED-STUDY OF CONCEPTION RATES', HUMAN REPRODUCTION, 9 330-333 (1994)
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1994 |
MCLAUGHLIN EA, FORD WCL, 'EFFECTS OF CRYOPRESERVATION ON THE INTRACELLULAR CALCIUM-CONCENTRATION OF HUMAN SPERMATOZOA AND ITS RESPONSE TO PROGESTERONE', MOLECULAR REPRODUCTION AND DEVELOPMENT, 37 241-246 (1994)
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1993 |
MCLAUGHLIN EA, FORD WCL, HULL MGR, 'EFFECTS OF CRYOPRESERVATION ON THE HUMAN SPERM ACROSOME AND ITS RESPONSE TO A23187', JOURNAL OF REPRODUCTION AND FERTILITY, 99 71-76 (1993)
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1993 |
McLaughlin E, Ford W, Hull MGR, 'The contribution of the toxicity of a glycerol-egg yolk-citrate cryopreservative to the decline in human sperm motility during cryopreservation', International Journal of Gynecology & Obstetrics, 41 207-207 (1993)
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1992 |
MCLAUGHLIN EA, FORD WCL, HULL MGR, 'MOTILITY CHARACTERISTICS AND MEMBRANE INTEGRITY OF CRYOPRESERVED HUMAN SPERMATOZOA', JOURNAL OF REPRODUCTION AND FERTILITY, 95 527-534 (1992)
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1992 |
MCLAUGHLIN EA, FORD WCL, HULL MGR, 'THE CONTRIBUTION OF THE TOXICITY OF A GLYCEROL EGG-YOLK CITRATE CRYOPRESERVATIVE TO THE DECLINE IN HUMAN SPERM MOTILITY DURING CRYOPRESERVATION', JOURNAL OF REPRODUCTION AND FERTILITY, 95 749-754 (1992)
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1992 |
FORD WCL, PONTING FA, MCLAUGHLIN EA, REES JM, HULL MGR, 'CONTROLLING THE SWIMMING SPEED OF HUMAN SPERM BY VARYING THE INCUBATION-TEMPERATURE AND ITS EFFECT ON CERVICAL-MUCUS PENETRATION', INTERNATIONAL JOURNAL OF ANDROLOGY, 15 127-134 (1992)
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1992 |
FORD WCL, MCLAUGHLIN EA, PRIOR SM, REES JM, WARDLE PG, HULL MGR, 'THE YIELD, MOTILITY AND PERFORMANCE IN THE HAMSTER EGG TEST OF HUMAN SPERMATOZOA PREPARED FROM CRYOPRESERVED SEMEN BY 4 DIFFERENT METHODS', HUMAN REPRODUCTION, 7 654-659 (1992)
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1991 |
FORD WCL, REES JM, MCLAUGHLIN EA, GODDARD RJ, HULL MGR, 'THE EFFECT OF A23187 CONCENTRATION AND EXPOSURE TIME ON THE OUTCOME OF THE HAMSTER EGG PENETRATION TEST', INTERNATIONAL JOURNAL OF ANDROLOGY, 14 127-139 (1991)
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1990 |
McLaughlin EA, Ford WC, 'A comparison of the freezing of human semen in the uncirculated vapour above liquid nitrogen and in a commercial semi-programmable freezer.', Human Reproduction, 5 724-728 (1990)
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1988 |
HARRIS MA, REES JM, MCLAUGHLIN EA, FORD WCL, WARDLE PG, HULL MGR, WATHES DC, 'AN EVALUATION OF THE ROLE OF RELAXIN IN THE PENETRATION OF CERVICAL-MUCUS BY SPERMATOZOA', HUMAN REPRODUCTION, 3 856-860 (1988)
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1987 |
WARDLE PG, MCLAUGHLIN E, SYKES JA, HULL MGR, 'INTRAUTERINE INSEMINATION', LANCET, 1 270-270 (1987)
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1987 |
WALKER A, GREGSON S, MCLAUGHLIN E, 'ATTITUDES TOWARDS DONOR INSEMINATION - A POST-WARNOCK SURVEY', HUMAN REPRODUCTION, 2 745-750 (1987)
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1987 |
RAY BD, MCDERMOTT A, WARDLE PG, CORRIGAN E, MITCHELL JD, MCLAUGHLIN EA, et al., 'INVITRO FERTILIZATION - FERTILIZATION FAILURE DUE TO TOXIC CATHETERS', JOURNAL OF IN VITRO FERTILIZATION AND EMBRYO TRANSFER, 4 58-61 (1987)
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1986 |
WARDLE PG, FOSTER PA, MITCHELL JD, MCLAUGHLIN EA, SYKES JAC, CORRIGAN E, et al., 'ENDOMETRIOSIS AND IVF - EFFECT OF PRIOR THERAPY', LANCET, 1 276-277 (1986)
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1986 |
WARDLE PG, FOSTER PA, MITCHELL JD, MCLAUGHLIN EA, WILLIAMS JAC, CORRIGAN E, et al., 'NORETHISTERONE TREATMENT TO CONTROL TIMING OF THE IVF CYCLE', HUMAN REPRODUCTION, 1 455-457 (1986)
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1986 |
WATHES DC, WARDLE PG, REES JM, MITCHELL JD, MCLAUGHLIN EA, HULL MGR, PORTER DG, 'IDENTIFICATION OF RELAXIN IMMUNOREACTIVITY IN HUMAN FOLLICULAR-FLUID', HUMAN REPRODUCTION, 1 515-517 (1986)
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1986 |
WARDLE PG, MCLAUGHLIN EA, WILLIAMS JAC, MITCHELL JD, FOSTER PA, CORRIGAN E, et al., 'SEMINAL ANALYSIS AND CLINICAL SPERM FUNCTION-TESTS - DEFINITION OF NORMAL VALUES USING INVITRO FERTILIZATION (IVF)', BRITISH JOURNAL OF OBSTETRICS AND GYNAECOLOGY, 93 1195-1195 (1986) |
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1985 |
WARDLE PG, MITCHELL JD, MCLAUGHLIN EA, RAY BD, MCDERMOTT A, HULL MGR, 'ENDOMETRIOSIS AND OVULATORY DISORDER - REDUCED FERTILIZATION INVITRO COMPARED WITH TUBAL AND UNEXPLAINED INFERTILITY', LANCET, 2 236-239 (1985)
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1983 |
MCLAUGHLIN EA, BROMWICH PD, MACKEN AM, WALKER AP, NEWTON JR, 'USE OF HOME INSEMINATION IN PROGRAMS OF ARTIFICIAL-INSEMINATION WITH DONOR SEMEN', BRITISH MEDICAL JOURNAL, 287 1110-1110 (1983)
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1979 |
MUSK AW, SMITH TJ, PETERS JM, MCLAUGHLIN E, 'PULMONARY-FUNCTION IN FIREFIGHTERS - ACUTE CHANGES IN VENTILATORY CAPACITY AND THEIR CORRELATES', BRITISH JOURNAL OF INDUSTRIAL MEDICINE, 36 29-34 (1979)
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1975 |
BARNETT WB, MCLAUGHLIN EA, 'ATOMIC-ABSORPTION DETERMINATION OF ANTIMONY, ARSENIC, BISMUTH, CADMIUM, LEAD, AND TIN IN IRON, COPPER, AND ZINC ALLOYS WITH GRAPHITE FURNACE', ANALYTICA CHIMICA ACTA, 80 285-296 (1975)
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