Biological Mass Spectrometry
This stream helps discover and quantify proteins, peptides, small molecules and metabolites. It supports biomedical, biological and environmental research.
Our top-of-the-line Mass Spectrometer combines three mass analysers into a single intelligent system. It is designed for higher throughput, while still able to discover and profile molecules from the most complex sample types in proteomics, metabolomics, small molecule and biopharmaceutical analysis.
Features
- Advanced experimental control utilising multiple analysers and MSn fragmentation techniques (HCD, ETD, CID).
- Flexible chromatography from 1nl/min to 100ul/min using the Vanquish Neo’s nano-, capillary-and micro-flow ranges
- Field asymmetric ion mobility spectrometry (FAIMS) to further separate complex mixtures
- Real-time searching to help identify and further manipulate peptides on the fly and improve TMT quant accuracy
- Proton transfer charge reduction (PTCR) to simplify intact large molecule analysis
- Electron transfer dissociation (ETD) for the identification of modified proteins and peptides
- A high mass range which extends the mass range from 2000 m/z to 8000 m/z for detection of large molecules
Combines a quadrupole mass filter with a high-field Orbitrap analyser. This hybrid configuration is ideal for discovery and quantitation from complex sample types that do not require the advanced fragmentation, computing and MSn features of our Eclipse Tribrid Orbitrap.
Features
- High-field Orbitrap analyser with resolving power up to 480,000
- Scan speeds up to 40Hz
- Field asymmetric ion mobility spectrometry (FAIMS) to further separate complex mixtures
- Nano-flow chromatography front end for separation of complex ‘omics mixtures
An older version of hybrid Quadrupole Orbitrap LC/MS. This system is dedicated to more basic workflows that do not require the advanced features of our latest generation of Orbitraps. Perfect for generating IDs from gel plugs or for High-Resolution accurate mass measurements.
Features
- Nano-flow chromatography front end for separation of complex ‘omics mixtures
- High-flow chromatography option available for High-res small molecule workflows
- Orbitrap analyser capable of a resolution of 280,000
The LC/MS of choice for fast and highly selective quantitation of specific target molecules within your samples.
The QTRAP 6500 can function as both a standard Triple Quadrupole for MRM based quantitation, or as a Linear Ion Trap for qualitative analysis.
Tackles a wide range of sample types including food and pharmaceutical testing, environmental monitoring, clinical and ‘omics’ quantitation.
Features
- High throughput and ultra-high-performance liquid chromatography front-end
- Flexible chromatography column chemistry options
- Reproducibly target up to 4,000 compounds in a single analysis with scheduled multiple reaction monitoring and 5msec polarity switching
- Quantify and confirm the identity of your targets by performing a Multiple Reaction Monitoring (MRM) triggered full scan MS/MS in the linear ion trap.
This system is dedicated to pre-fractionate samples ‘offline’ prior to their introduction to our online Orbitrap LC/MS systems. HILIC and high-pH fractionation options are both available.
This technique is used where the maximum identification of peptides/proteins is required from extremely complex sample types.
The University of Newcastle acknowledges the traditional custodians of the lands within our footprint areas: Awabakal, Darkinjung, Biripai, Worimi, Wonnarua, and Eora Nations. We also pay respect to the wisdom of our Elders past and present.