Flow cytometry

The flow cytometry sub-unit of the ABRF is available for analysis of multiple cellular and particle parameters, and additionally for fluorescence activated cell sorting.

The sub-unit currently comprises six instruments across two locations, the University of Newcastle and Hunter Medical Research Institute (HMRI).

Cell analysis

Flow cytometry has many applications, including:

  • Immunophenotyping
  • Cellular metabolism studies: including changes in intracellular calcium levels, cellular pH and changes in membrane potential
  • DNA analysis/ploidy/karyotyping: Studies of changes in DNA/RNA levels using DNA-binding dyes such as Ethidium bromide
  • Cytotoxicity studies: including studies of Natural killer cell activity and Cytotoxic T lymphocyte (CTL) activity
  • Cell signalling

Detailed information on equipment

The University of Newcastle acknowledges the traditional custodians of the lands within our footprint areas: Awabakal, Darkinjung, Biripai, Worimi, Wonnarua, and Eora Nations. We also pay respect to the wisdom of our Elders past and present.