2024 |
Fielding D, Lakis V, Dalley AJ, Chittoory H, Newell F, Koufariotis LT, et al., 'Evaluation of Endobronchial Ultrasound-Guided Transbronchial Needle Aspiration (EBUS-TBNA) Samples from Advanced Non-Small Cell Lung Cancer for Whole Genome, Whole Exome and Comprehensive Panel Sequencing.', Cancers (Basel), 16 (2024)
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2024 |
Mackintosh JA, Keir G, Troy LK, Holland AE, Grainge C, Chambers DC, et al., 'Treatment of idiopathic pulmonary fibrosis and progressive pulmonary fibrosis: A position statement from the Thoracic Society of Australia and New Zealand 2023 revision.', Respirology, 29 105-135 (2024) [C1]
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2023 |
Kritikos V, Harvey ES, Stevens S, Katelaris CH, Langton D, Rimmer J, et al., 'Comorbidities Modify the Phenotype but Not the Treatment Effectiveness to Mepolizumab in Severe Eosinophilic Asthma.', J Allergy Clin Immunol Pract, 11 885-895.e13 (2023) [C1]
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Nova |
2023 |
Gibson PG, Urroz Guerrero PD, Poon C, Rutherford N, Brooker B, Smith A, et al., 'Ventilation Heterogeneity Is a Treatable Trait in Severe Asthma.', J Allergy Clin Immunol Pract, (2023) [C1]
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2023 |
Veerati PC, Reid AT, Nichol KS, Wark PAB, Knight DA, Bartlett NW, Grainge CL, 'Mechanical forces suppress antiviral innate immune responses from asthmatic airway epithelial cells following rhinovirus infection.', Am J Physiol Lung Cell Mol Physiol, 325 L206-L214 (2023) [C1]
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Nova |
2023 |
Ramsahai JM, Simpson JL, Cook A, Gibson PG, McDonald V, Grainge C, et al., 'Randomised controlled trial for the titration of oral corticosteroids using markers of inflammation in severe asthma.', Thorax, 78 868-874 (2023) [C1]
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Nova |
2023 |
West A, Chaudhuri N, Barczyk A, Wilsher ML, Hopkins P, Glaspole I, et al., 'Inhaled pirfenidone solution (AP01) for IPF: a randomised, open-label, dose-response trial', THORAX, [C1]
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2023 |
Awatade NT, Reid AT, Nichol KS, Budden KF, Veerati PC, Pathinayake PS, et al., 'Comparison of commercially available differentiation media on cell morphology, function, and anti-viral responses in conditionally reprogrammed human bronchial epithelial cells.', Scientific reports, 13 11200 (2023) [C1]
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Nova |
2023 |
Barnes H, Chambers D, Grainge C, Corte TJ, Bastiampillai S, Frenkel S, et al., 'Clinical utility of a standardized chronic hypersensitivity pneumonitis exposure questionnaire', RESPIROLOGY, 28 366-372 (2023) [C1]
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Nova |
2023 |
Jee AS, Stewart I, Youssef P, Adelstein S, Lai D, Hua S, et al., 'A Composite Serum Biomarker Index for the Diagnosis of Systemic Sclerosis-Associated Interstitial Lung Disease: A Multicenter, Observational Cohort Study.', Arthritis Rheumatol, 75 1424-1433 (2023) [C1]
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2023 |
Lucas SEM, Raspin K, Mackintosh J, Glaspole I, Reynolds PN, Chia C, et al., 'Preclinical interstitial lung disease in relatives of familial pulmonary fibrosis patients', PULMONOLOGY, 29 257-260
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2022 |
Teoh AKY, Holland AE, Morisset J, Flaherty KR, Wells AU, Walsh SLF, et al., 'Essential Features of an Interstitial Lung Disease Multidisciplinary Meeting An International Delphi Survey', Annals of the American Thoracic Society, 19 66-73 (2022) [C1]
Rationale: The interstitial lung disease (ILD) multidisciplinary meetings (MDM), composed of pulmonologists, radiologists, and pathologists, is integral to the rendering of an acc... [more]
Rationale: The interstitial lung disease (ILD) multidisciplinary meetings (MDM), composed of pulmonologists, radiologists, and pathologists, is integral to the rendering of an accurate ILD diagnosis. However, there is significant heterogeneity in the conduct of ILD MDMs, and questions regarding their best practices remain unanswered. Objectives: To achieve consensus among ILD experts on essential components of an ILD MDM. Methods: Using a Delphi methodology, semi-structured interviews with ILD experts were used to identify key themes and features of ILD MDMs. These items informed two subsequent rounds of online questionnaires that were used to achieve consensus among a broader, international panel of ILD experts. Experts were asked to rate their level of agreement on a five-point Likert scale. An a priori threshold for consensus was set at a median score 4 or 5 with an interquartile range of 0. Results: We interviewed 15 ILD experts, and 102 ILD experts participated in the online questionnaires. Five items and two exploratory statements achieved consensus on being essential for an ILD MDM following two questionnaire rounds. There was consensus that the presence of at least one radiologist, a quiet setting with a visual projection system, a high-quality chest high-resolution computed tomography, and a standardized template summarizing collated patient data are essential components of an ILD MDM. Experts also agreed that it would be useful for ILD MDMs to undergo an annual benchmarking process and a validation process by fulfilling a minimum number of cases annually. Twenty-seven additional features were considered to be either highly desirable or desirable features based on the degree of consensus. Although our findings on desirable features are similar to the current literature, several of these remain controversial and warrant further research. The study also showed an agreement among participants on several future concepts to improve the ILD MDM, such as performing regular self-assessments and conducting research into shared practices to develop an international expert guideline statement on ILD MDMs. Conclusions: This Delphi study showed consensus among international ILD experts on essential and desirable features of an ILD MDM. Our data represent an important step toward potential collaborative research into future standardization of ILD MDMs.
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2022 |
Williams TC, Loo S-L, Nichol KS, Reid AT, Veerati PC, Esneau C, et al., 'IL-25 blockade augments antiviral immunity during respiratory virus infection', COMMUNICATIONS BIOLOGY, 5 (2022) [C1]
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Nova |
2022 |
Veerati PC, Nichol KS, Read JM, Bartlett NW, Wark PAB, Knight DA, et al., 'Conditionally reprogrammed asthmatic bronchial epithelial cells express lower
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Nova |
2022 |
Kan S, Grainge C, Nichol K, Reid A, Knight D, Sun Y, et al., 'TLR7 agonist loaded airway epithelial targeting nanoparticles stimulate innate immunity and suppress viral replication in human bronchial epithelial cells', INTERNATIONAL JOURNAL OF PHARMACEUTICS, 617 (2022) [C1]
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2022 |
Clynick B, Corte TJ, Jo HE, Stewart I, Glaspole IN, Grainge C, et al., 'Biomarker signatures for progressive idiopathic pulmonary fibrosis', EUROPEAN RESPIRATORY JOURNAL, 59 (2022) [C1]
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2022 |
Prêle CM, Miles T, Pearce DR, O'Donoghue RJ, Grainge C, Barrett L, et al., 'Plasma cell but not CD20-mediated B-cell depletion protects from bleomycin-induced lung fibrosis.', The European respiratory journal, 60 2101469 (2022) [C1]
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2022 |
Humphries SM, Mackintosh JA, Jo HE, Walsh SLF, Silva M, Calandriello L, et al., 'Quantitative computed tomography predicts outcomes in idiopathic pulmonary fibrosis', Respirology, 27 1045-1053 (2022) [C1]
Background and objective: Prediction of disease course in patients with progressive pulmonary fibrosis remains challenging. The purpose of this study was to assess the prognostic ... [more]
Background and objective: Prediction of disease course in patients with progressive pulmonary fibrosis remains challenging. The purpose of this study was to assess the prognostic value of lung fibrosis extent quantified at computed tomography (CT) using data-driven texture analysis (DTA) in a large cohort of well-characterized patients with idiopathic pulmonary fibrosis (IPF) enrolled in a national registry. Methods: This retrospective analysis included participants in the Australian IPF Registry with available CT between 2007 and 2016. CT scans were analysed using the DTA method to quantify the extent of lung fibrosis. Demographics, longitudinal pulmonary function and quantitative CT metrics were compared using descriptive statistics. Linear mixed models, and Cox analyses adjusted for age, gender, BMI, smoking history and treatment with anti-fibrotics were performed to assess the relationships between baseline DTA, pulmonary function metrics and outcomes. Results: CT scans of 393 participants were analysed, 221 of which had available pulmonary function testing obtained within 90 days of CT. Linear mixed-effect modelling showed that baseline DTA score was significantly associated with annual rate of decline in forced vital capacity and diffusing capacity of carbon monoxide. In multivariable Cox proportional hazard models, greater extent of lung fibrosis was associated with poorer transplant-free survival (hazard ratio [HR] 1.20, p¿< 0.0001) and progression-free survival (HR 1.14, p¿< 0.0001). Conclusion: In a multi-centre observational registry of patients with IPF, the extent of fibrotic abnormality on baseline CT quantified using DTA is associated with outcomes independent of pulmonary function.
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2022 |
Ramarao-Milne P, Kondrashova O, Patch AM, Nones K, Koufariotis LT, Newell F, et al., 'Comparison of actionable events detected in cancer genomes by whole-genome sequencing, in silico whole-exome and mutation panels', ESMO Open, 7 (2022) [C1]
Background: Next-generation sequencing is used in cancer research to identify somatic and germline mutations, which can predict sensitivity or resistance to therapies, and may be ... [more]
Background: Next-generation sequencing is used in cancer research to identify somatic and germline mutations, which can predict sensitivity or resistance to therapies, and may be a useful tool to reveal drug repurposing opportunities between tumour types. Multigene panels are used in clinical practice for detecting targetable mutations. However, the value of clinical whole-exome sequencing (WES) and whole-genome sequencing (WGS) for cancer care is less defined, specifically as the majority of variants found using these technologies are of uncertain significance. Patients and methods: We used the Cancer Genome Interpreter and WGS in 726 tumours spanning 10 cancer types to identify drug repurposing opportunities. We compare the ability of WGS to detect actionable variants, tumour mutation burden (TMB) and microsatellite instability (MSI) by using in silico down-sampled data to mimic WES, a comprehensive sequencing panel and a hotspot mutation panel. Results: We reveal drug repurposing opportunities as numerous biomarkers are shared across many solid tumour types. Comprehensive panels identify the majority of approved actionable mutations, with WGS detecting more candidate actionable mutations for biomarkers currently in clinical trials. Moreover, estimated values for TMB and MSI vary when calculated from WGS, WES and panel data, and are dependent on whether all mutations or only non-synonymous mutations were used. Our results suggest that TMB and MSI thresholds should not only be tumour-dependent, but also be sequencing platform-dependent. Conclusions: There is a large opportunity to repurpose cancer drugs, and these data suggest that comprehensive sequencing is an invaluable source of information to guide clinical decisions by facilitating precision medicine and may provide a wealth of information for future studies. Furthermore, the sequencing and analysis approach used to estimate TMB may have clinical implications if a hard threshold is used to indicate which patients may respond to immunotherapy.
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2022 |
Alam MZ, Simonetti A, Brillantino R, Tayler N, Grainge C, Siribaddana P, et al., 'Predicting Pulmonary Function From the Analysis of Voice: A Machine Learning Approach', Frontiers in Digital Health, 4 (2022) [C1]
Introduction: To self-monitor asthma symptoms, existing methods (e.g. peak flow metre, smart spirometer) require special equipment and are not always used by the patients. Voice r... [more]
Introduction: To self-monitor asthma symptoms, existing methods (e.g. peak flow metre, smart spirometer) require special equipment and are not always used by the patients. Voice recording has the potential to generate surrogate measures of lung function and this study aims to apply machine learning approaches to predict lung function and severity of abnormal lung function from recorded voice for asthma patients. Methods: A threshold-based mechanism was designed to separate speech and breathing from 323 recordings. Features extracted from these were combined with biological factors to predict lung function. Three predictive models were developed using Random Forest (RF), Support Vector Machine (SVM), and linear regression algorithms: (a) regression models to predict lung function, (b) multi-class classification models to predict severity of lung function abnormality, and (c) binary classification models to predict lung function abnormality. Training and test samples were separated (70%:30%, using balanced portioning), features were normalised, 10-fold cross-validation was used and model performances were evaluated on the test samples. Results: The RF-based regression model performed better with the lowest root mean square error of 10·86. To predict severity of lung function impairment, the SVM-based model performed best in multi-class classification (accuracy = 73.20%), whereas the RF-based model performed best in binary classification models for predicting abnormal lung function (accuracy = 85%). Conclusion: Our machine learning approaches can predict lung function, from recorded voice files, better than published approaches. This technique could be used to develop future telehealth solutions including smartphone-based applications which have potential to aid decision making and self-monitoring in asthma.
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Nova |
2022 |
Walsh SLF, Mackintosh JA, Calandriello L, Silva M, Sverzellati N, Larici AR, et al., 'Deep Learning-based Outcome Prediction in Progressive Fibrotic Lung Disease Using High-Resolution Computed Tomography.', Am J Respir Crit Care Med, 206 883-891 (2022) [C1]
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2021 |
Thomas D, Harvey ES, McDonald VM, Stevens S, Upham JW, Katelaris CH, et al., 'Mepolizumab and Oral Corticosteroid Stewardship: Data from the Australian Mepolizumab Registry', Journal of Allergy and Clinical Immunology: In Practice, 9 2715-2724.e5 (2021) [C1]
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Nova |
2021 |
Girkin J, Loo S-L, Esneau C, Maltby S, Mercuri F, Chua B, et al., 'TLR2-mediated innate immune priming boosts lung anti-viral immunity', EUROPEAN RESPIRATORY JOURNAL, 58 (2021) [C1]
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Nova |
2021 |
Schuliga M, Kanwal A, Read J, Blokland KEC, Burgess JK, Prele CM, et al., 'A cGAS-dependent response links DNA damage and senescence in alveolar epithelial cells: a potential drug target in IPF', AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY, 321 L859-L871 (2021) [C1]
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Nova |
2021 |
Cooper WA, Mahar A, Myers JL, Grainge C, Corte TJ, Williamson JP, et al., 'Untitled', ARCHIVES OF PATHOLOGY & LABORATORY MEDICINE, 145 1326-1327 (2021)
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2021 |
Mackintosh JA, Pietsch M, Lutzky V, Enever D, Bancroft S, Apte SH, et al., 'TELO-SCOPE study: a randomised, double-blind, placebo-controlled, phase 2 trial of danazol for short telomere related pulmonary fibrosis', BMJ OPEN RESPIRATORY RESEARCH, 8 (2021)
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2021 |
Mackintosh JA, Glenn L, Barnes H, Dunn E, Bancroft S, Reddy T, et al., 'Benefits of a virtual interstitial lung disease multidisciplinary meeting in the face of COVID-19', RESPIROLOGY, 26 612-615 (2021)
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2021 |
Thomashow BM, Mannino DM, Tal-Singer R, Crapo JD, 'A rapidly changing understanding of COPD: World COPD Day from the COPD Foundation', American Journal of Physiology - Lung Cellular and Molecular Physiology, 321 L983-L987 (2021)
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2021 |
Cooper WA, Mahar A, Myers JL, Grainge C, Corte TJ, Williamson JP, et al., 'Cryobiopsy for Identification of Usual Interstitial Pneumonia and Other Interstitial Lung Disease Features', AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, 203 1306-1313 (2021) [C1]
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Nova |
2021 |
Maltby S, McDonald VM, Upham JW, Bowler SD, Chung LP, Denton EJ, et al., 'Severe asthma assessment, management and the organisation of care in Australia and New Zealand: expert forum roundtable meetings', INTERNAL MEDICINE JOURNAL, 51 169-180 (2021) [C1]
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Nova |
2021 |
Cooper WA, Mahar A, Myers JL, Grainge C, Corte TJ, Williamson JP, et al., 'Letters to the Editor', Archives of Pathology and Laboratory Medicine, 145 1326-1327 (2021)
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2021 |
Jee AS, Sheehy R, Hopkins P, Corte TJ, Grainge C, Troy LK, et al., 'Diagnosis and management of connective tissue disease-associated interstitial lung disease in Australia and New Zealand: A position statement from the Thoracic Society of Australia and New Zealand*', RESPIROLOGY, 26 23-51 (2021)
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2021 |
Twaddell SH, Gibson PG, Grainge C, Baines KJ, 'Parapneumonic Effusions Are Characterized by Elevated Levels of Neutrophil Extracellular Traps', CHEST, 160 1645-1655 (2021) [C1]
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Nova |
2021 |
Waters DW, Schuliga M, Pathinayake PS, Wei L, Tan H-Y, Blokland KEC, et al., 'A Senescence Bystander Effect in Human Lung Fibroblasts', BIOMEDICINES, 9 (2021) [C1]
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Nova |
2020 |
Schuliga M, Read J, Blokland KEC, Waters DW, Burgess J, Prele C, et al., 'Self DNA perpetuates IPF lung fibroblast senescence in a cGAS-dependent manner', CLINICAL SCIENCE, 134 889-905 (2020) [C1]
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Nova |
2020 |
Veerati PC, Mitchel JA, Reid AT, Knight DA, Bartlett NW, Park JA, Grainge CL, 'Airway mechanical compression: Its role in asthma pathogenesis and progression', European Respiratory Review, 29 1-13 (2020) [C1]
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Nova |
2020 |
Reid AT, Nichol KS, Veerati PC, Moheimani F, Kicic A, Stick SM, et al., 'Blocking notch3 signaling abolishes MUC5AC production in airway epithelial cells from individuals with asthma', American Journal of Respiratory Cell and Molecular Biology, 62 513-523 (2020) [C1]
In asthma, goblet cell numbers are increased within the airway epithelium, perpetuating the production of mucus that is more difficult to clear and results in airway mucus pluggin... [more]
In asthma, goblet cell numbers are increased within the airway epithelium, perpetuating the production of mucus that is more difficult to clear and results in airway mucus plugging. Notch1, Notch2, or Notch3, or a combination of these has been shown to influence the differentiation of airway epithelial cells. How the expression of specific Notch isoforms differs in fully differentiated adult asthmatic epithelium and whether Notch influences mucin production after differentiation is currently unknown. We aimed to quantify different Notch isoforms in the airway epithelium of individuals with severe asthma and to examine the impact of Notch signaling on mucin MUC5AC. Human lung sections and primary bronchial epithelial cells from individuals with and without asthma were used in this study. Primary bronchial epithelial cells were differentiated at the air-liquid interface for 28 days. Notch isoform expression was analyzed by Taqman quantitative PCR. Immunohistochemistry was used to localize and quantify Notch isoforms in human airway sections. Notch signaling was inhibited in vitro using dibenzazepine or Notch3-specific siRNA, followed by analysis of MUC5AC. NOTCH3 was highly expressed in asthmatic airway epithelium compared with nonasthmatic epithelium. Dibenzazepine significantly reduced MUC5AC production in air-liquid interface cultures of primary bronchial epithelial cells concomitantly with suppression of NOTCH3 intracellular domain protein. Specific knockdown using NOTCH3 siRNA recapitulated the dibenzazepine-induced reduction in MUC5AC. We demonstrate that NOTCH3 is a regulator of MUC5AC production. Increased NOTCH3 signaling in the asthmatic airway epithelium may therefore be an underlying driver of excess MUC5AC production.
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Nova |
2020 |
Blokland KEC, Waters DW, Schuliga M, Read J, Pouwels SD, Grainge CL, et al., 'Senescence of IPF Lung Fibroblasts Disrupt Alveolar Epithelial Cell Proliferation and Promote Migration in Wound Healing', PHARMACEUTICS, 12 (2020) [C1]
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Nova |
2020 |
Troy LK, Grainge C, Corte TJ, Williamson JP, Vallely MP, Cooper WA, et al., 'Diagnostic accuracy of transbronchial lung cryobiopsy for interstitial lung disease diagnosis (COLDICE): a prospective, comparative study', The Lancet Respiratory Medicine, 8 171-181 (2020) [C1]
Background: Transbronchial lung cryobiopsy (TBLC) is a novel technique for sampling lung tissue for interstitial lung disease diagnosis. The aim of this study was to establish the... [more]
Background: Transbronchial lung cryobiopsy (TBLC) is a novel technique for sampling lung tissue for interstitial lung disease diagnosis. The aim of this study was to establish the diagnostic accuracy of TBLC compared with surgical lung biopsy (SLB), in the context of increasing use of TBLC in clinical practice as a less invasive biopsy technique. Methods: COLDICE was a prospective, multicentre, diagnostic accuracy study investigating diagnostic agreement between TBLC and SLB, across nine Australian tertiary hospitals. Patients with interstitial lung disease aged between 18 and 80 years were eligible for inclusion if they required histopathological evaluation to aid diagnosis, after detailed baseline evaluation. After screening at a centralised multidisciplinary discussion (MDD), patients with interstitial lung disease referred for lung biopsy underwent sequential TBLC and SLB under one anaesthetic. Each tissue sample was assigned a number between 1 and 130, allocated in a computer-generated random sequence. Encoded biopsy samples were then analysed by masked pathologists. At subsequent MDD, de-identified cases were discussed twice with either TBLC or SLB along with clinical and radiological data, in random non-consecutive order. Co-primary endpoints were agreement of histopathological features in TBLC and SLB for patterns of definite or probable usual interstitial pneumonia, indeterminate for usual interstitial pneumonia, and alternative diagnosis; and for agreement of consensus clinical diagnosis using TBLC and SLB at MDD. Concordance and ¿ values were calculated for each primary endpoint. This study is registered with the Australian New Zealand Clinical Trials Registry, ACTRN12615000718549. Findings: Between March 15, 2016, and April 15, 2019, we enrolled 65 patients (31 [48%] men, 34 [52%] women; mean age 66·1 years [SD 9·3]; forced vital capacity 83·7% [SD 14·2]; diffusing capacity for carbon monoxide 63·4% [SD 12·8]). TBLC (7·1 mm, SD 1·9) and SLB (46·5 mm, 14·9) samples were each taken from two separate ipsilateral lobes. Histopathological agreement between TBLC and SLB was 70·8% (weighted ¿ 0·70, 95% CI 0·55¿0·86); diagnostic agreement at MDD was 76·9% (¿ 0·62, 0·47¿0·78). For TBLC with high or definite diagnostic confidence at MDD (39 [60%] of 65 cases), 37 (95%) were concordant with SLB diagnoses. In the 26 (40%) of 65 cases with low-confidence or unclassifiable TBLC diagnoses, SLB reclassified six (23%) to alternative high-confidence or definite MDD diagnoses. Mild-moderate airway bleeding occurred in 14 (22%) patients due to TBLC. The 90-day mortality was 2% (one of 65 patients), following acute exacerbation of idiopathic pulmonary fibrosis. Interpretation: High levels of agreement between TBLC and SLB for both histopathological interpretation and MDD diagnoses were shown. The TBLC MDD diagnoses made with high confidence were particularly reliable, showing excellent concordance with SLB MDD diagnoses. These data support the clinical utility of TBLC in interstitial lung disease diagnostic algorithms. Further studies investigating the safety profile of TBLC are needed. Funding: University of Sydney, Hunter Medical Research Institute, Erbe Elektromedizin, Medtronic, Cook Medical, Rymed, Karl-Storz, Zeiss, and Olympus.
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2020 |
Abramson MJ, Murambadoro T, Alif SM, Benke GP, Dharmage SC, Glaspole I, et al., 'Occupational and environmental risk factors for idiopathic pulmonary fibrosis in Australia: case-control study', THORAX, 75 864-869 (2020) [C1]
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2020 |
Moore I, Wrobel J, Rhodes J, Lin Q, Webster S, Jo H, et al., 'Australasian interstitial lung disease registry (AILDR): objectives, design and rationale of a bi-national prospective database', BMC PULMONARY MEDICINE, 20 (2020) [C1]
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2020 |
Lau EMT, Grainge C, Williamson JP, Corte TJ, Cooper WA, Phillips MJ, et al., 'Methodologies of COLDICE and Cryo-PID studies: details make the difference', ANNALS OF TRANSLATIONAL MEDICINE, 8 (2020)
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2020 |
Knight DA, Grainge CL, Stick SM, Kicic A, Schuliga M, 'Epithelial Mesenchymal Transition in Respiratory Disease: Fact or Fiction', Chest, 157 1591-1596 (2020) [C1]
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Nova |
2020 |
Veerati PC, Troy NM, Reid AT, Li NF, Nichol KS, Kaur P, et al., 'Airway Epithelial Cell Immunity Is Delayed During Rhinovirus Infection in Asthma and COPD', FRONTIERS IN IMMUNOLOGY, 11 (2020) [C1]
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Nova |
2020 |
Clynick B, Jo HE, Corte TJ, Glaspole IN, Grainge C, Hopkins PMA, et al., 'Circulating RNA differences between patients with stable and progressive idiopathic pulmonary fibrosis', EUROPEAN RESPIRATORY JOURNAL, 56 (2020) [C1]
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Nova |
2020 |
Harvey ES, Langton D, Katelaris C, Stevens S, Farah CS, Gillman A, et al., 'Mepolizumab effectiveness and identification of super-responders in severe asthma', EUROPEAN RESPIRATORY JOURNAL, 55 (2020) [C1]
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Nova |
2020 |
Kan S, Hariyadi DM, Grainge C, Knight DA, Bartlett NW, Liang M, 'Airway epithelial-targeted nanoparticles for asthma therapy', AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY, 318 L500-L509 (2020) [C1]
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Nova |
2019 |
Clynick B, Jo HE, Corte TJ, Glaspole IN, Grainge C, Hopkins PMA, et al., 'Circulating RNA differences between patients with stable and progressive lung function in IPF (2019) (vol 54, 1802115, 2019)', EUROPEAN RESPIRATORY JOURNAL, 54 CP8-CP8 (2019)
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2019 |
Veerati PC, Grainge C, 'Peering deeper into asthmatic lungs', RESPIROLOGY, 24 1037-1038 (2019)
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2019 |
Jo HE, Corte TJ, Glaspole I, Grainge C, Hopkins PMA, Moodley Y, et al., 'Gastroesophageal reflux and antacid therapy in IPF: analysis from the Australia IPF Registry', BMC PULMONARY MEDICINE, 19 (2019) [C1]
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Nova |
2019 |
Twaddell SH, Baines KJ, Grainge C, Gibson PG, 'The Emerging Role of Neutrophil Extracellular Traps in Respiratory Disease', Chest, 156 774-782 (2019) [C1]
Neutrophil extracellular traps (NETs) are extrusions of intracellular DNA and attached granular material that enable bacterial killing. NETs are increasingly recognized for their ... [more]
Neutrophil extracellular traps (NETs) are extrusions of intracellular DNA and attached granular material that enable bacterial killing. NETs are increasingly recognized for their role in the pathogenesis of respiratory disease. NETs are composed of a complex mix of intracellularly derived material that neutrophils organize within the cytoplasm and then expel in a nondirected manner in the vicinity of invading organisms. Combined, these trap and destroy multiple genera of microbes including bacteria, fungi, viruses, and protozoans, limiting infection especially where phagocytosis is not possible. At first, NET formation was thought to be a terminal event for neutrophils; however, it is now apparent that some neutrophils survive this process, becoming anuclear, and may drive ongoing tissue damage. NETs are now known to be directly cytotoxic to lung epithelium and endothelium, and their excessive production is seen in pneumonia and acute lung injury as well as several chronic diseases, including COPD, asthma, and cystic fibrosis. NETs also appear to play a role in both tumor defense and dissemination, depending on the local microenvironment and the specific tumor subtype. It is becoming increasingly apparent that NET formation can exert a positive or negative influence on multiple respiratory pathologies and that simply globally reducing or increasing NET formation is unlikely to be a therapeutic success. Rather, as our understanding grows, it is likely that targeted NET up- or downregulation along with destruction or protection of already formed NETs may become an additional point of intervention for respiratory physicians.
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Nova |
2019 |
Burgess A, Goon K, Brannan JD, Attia J, Palazzi K, Oldmeadow C, et al., 'Eligibility for anti-fibrotic treatment in idiopathic pulmonary fibrosis depends on the predictive equation used for pulmonary function testing', Respirology, 24 988-995 (2019) [C1]
Background and objective: Publicly funded therapy for idiopathic pulmonary fibrosis (IPF) relies on percentage predicted values from pulmonary function testing, for example Austra... [more]
Background and objective: Publicly funded therapy for idiopathic pulmonary fibrosis (IPF) relies on percentage predicted values from pulmonary function testing, for example Australian patients must have a forced vital capacity =50% (%FVC), transfer factor of the lung for carbon monoxide = 30% (%TLco) and forced expiratory volume in 1 s (FEV1)/FVC ratio > 0.7. Despite defined cut-off values, no jurisdiction prescribes a reference equation for use; multiple equations exist. We hypothesized that access to subsidized treatment varies depending on the chosen equation. The %FVC and %TLco from different commonly used reference equations across general respiratory patients, and IPF-specific patients, were compared. Methods: FVC and TLco measurements from a large general respiratory laboratory and the Australian Idiopathic Pulmonary Fibrosis Registry (AIPFR) database were analysed using multiple equations. Differences between %FVC and %TLco for each equation were calculated, with particular interest in classification of patients (%) at the threshold for subsidized treatment. Results: A total of 20 378 general respiratory database results were analysed. The %FVC = 50% increased from 86% with the Roca equation to 96% with Quanjer (European Coal and Steal Community, ECSC) and %TLco=30% increased from 91% with Paoletti to 98% with Thompson. However, overall increase in eligibility for subsidized treatment was modest, varying from 48.2% to 49.2%. A total of 545 AIPFR database results were analysed. The %FVC = 50% increased from 73% with Roca to 94% with Quanjer (ECSC) and %TLco=30% increased from 87% with Paoletti to 96% with Miller. Overall eligibility for subsidized treatment in the AIPFR group varied from 73.6% to 82.8% between surveyed interstitial lung disease (ILD) centres based entirely on the equation used. Conclusion: Substantial variability exists between reference equations, impacting access to subsidized treatment. Treating clinicians should be aware of this when assessing patients around public funding thresholds.
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Nova |
2019 |
Troy LK, Grainge C, Corte T, Williamson JP, Vallely MP, Cooper W, et al., 'Cryobiopsy versus open lung biopsy in the diagnosis of interstitial lung disease (COLDICE): protocol of a multicentre study', BMJ OPEN RESPIRATORY RESEARCH, 6 (2019)
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2019 |
Jo HE, Glaspole I, Goh N, Hopkins PMA, Moodley Y, Reynolds PN, et al., 'Implications of the diagnostic criteria of idiopathic pulmonary fibrosis in clinical practice: Analysis from the Australian Idiopathic Pulmonary Fibrosis Registry', RESPIROLOGY, 24 361-368 (2019) [C1]
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Nova |
2019 |
Waters DW, Blokland KEC, Pathinayake PS, Wei L, Schuliga M, Jaffar J, et al., 'STAT3 Regulates the Onset of Oxidant-induced Senescence in Lung Fibroblasts.', Am J Respir Cell Mol Biol, 61 61-73 (2019) [C1]
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Nova |
2019 |
Singanayagam A, Loo SL, Calderazzo M, Finney LJ, Torralbo MBT, Bakhsoliani E, et al., 'Antiviral immunity is impaired in COPD patients with frequent exacerbations', American Journal of Physiology - Lung Cellular and Molecular Physiology, 317 L893-L903 (2019) [C1]
Patients with frequent exacerbations represent a chronic obstructive pulmonary disease (COPD) subgroup requiring better treatment options. The aim of this study was to determine t... [more]
Patients with frequent exacerbations represent a chronic obstructive pulmonary disease (COPD) subgroup requiring better treatment options. The aim of this study was to determine the innate immune mechanisms that underlie susceptibility to frequent exacerbations in COPD. We measured sputum expression of immune mediators and bacterial loads in samples from patients with COPD at stable state and during virusassociated exacerbations. In vitro immune responses to rhinovirus infection in differentiated primary bronchial epithelial cells (BECs) sampled from patients with COPD were additionally evaluated. Patients were stratified as frequent exacerbators (=2 exacerbations in the preceding year) or infrequent exacerbators (<2 exacerbations in the preceding year) with comparisons made between these groups. Frequent exacerbators had reduced sputum cell mRNA expression of the antiviral immune mediators type I and III interferons and reduced interferon-stimulated gene (ISG) expression when clinically stable and during virus-associated exacerbation. A role for epithelial cellintrinsic innate immune dysregulation was identified: induction of interferons and ISGs during in vitro rhinovirus (RV) infection was also impaired in differentiated BECs from frequent exacerbators. Frequent exacerbators additionally had increased sputum bacterial loads at 2 wk following virus-associated exacerbation onset. These data implicate deficient airway innate immunity involving epithelial cells in the increased propensity to exacerbations observed in some patients with COPD. Therapeutic approaches to boost innate antimicrobial immunity in the lung could be a viable strategy for prevention and treatment of frequent exacerbations.
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Nova |
2019 |
Vertigan AE, Grainge CL, 'Case 1', ERS Monograph, 2019 334-337 (2019)
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2018 |
Reid AT, Veerati PC, Gosens R, Bartlett NW, Wark PA, Grainge CL, et al., 'Persistent induction of goblet cell differentiation in the airways: Therapeutic approaches', Pharmacology and Therapeutics, 185 155-169 (2018) [C1]
Dysregulated induction of goblet cell differentiation results in excessive production and retention of mucus and is a common feature of several chronic airways diseases. To date, ... [more]
Dysregulated induction of goblet cell differentiation results in excessive production and retention of mucus and is a common feature of several chronic airways diseases. To date, therapeutic strategies to reduce mucus accumulation have focused primarily on altering the properties of the mucus itself, or have aimed to limit the production of mucus-stimulating cytokines. Here we review the current knowledge of key molecular pathways that are dysregulated during persistent goblet cell differentiation and highlights both pre-existing and novel therapeutic strategies to combat this pathology.
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Nova |
2018 |
Waters DW, Blokland KEC, Pathinayake PS, Burgess JK, Mutsaers SE, Prele CM, et al., 'Fibroblast senescence in the pathology of idiopathic pulmonary fibrosis', American Journal of Physiology - Lung Cellular and Molecular Physiology, 315 L162-L172 (2018) [C1]
Idiopathic pulmonary fibrosis (IPF) is a chronic fibrosing interstitial pneu monia of unknown cause with a median survival of only three years. Little is known about the mechanism... [more]
Idiopathic pulmonary fibrosis (IPF) is a chronic fibrosing interstitial pneu monia of unknown cause with a median survival of only three years. Little is known about the mechanisms that precede the excessive collagen deposition seen in IPF, but cellular senescence has been strongly implicated in disease pathology. Senescence is a state of irreversible cell-cycle arrest accompanied by an abnormal secretory profile and is thought to play a critical role in both development and wound repair. Normally, once a senescent cell has contributed to wound repair, it is promptly removed from the environment via infiltrating immune cells. However, if immune clearance fails, the persistence of senescent cells is thought to drive disease pathology through their altered secretory profile. One of the major cell types involved in wound healing is fibroblasts, and senescent fibroblasts have been identified in the lungs of patients with IPF and in fibroblast cultures from IPF lungs. The question of what is driving abnormally high numbers of fibroblasts into senescence remains unanswered. The transcription factor signal transducer and activator of transcription 3 (STAT3) plays a role in a myriad of processes, including cell-cycle progression, gene transcription, as well as mitochondrial respiration, all of which are dysregulated during senescence. Activation of STAT3 has previously been shown to correlate with IPF progression and therefore is a potential molecular target to modify early-stage senescence and restore normal fibroblast function. This review summarizes what is presently known about fibroblast senescence in IPF and how STAT3 may contribute to this phenotype.
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Nova |
2018 |
Schuliga M, Pechkovsky DV, Read J, Waters DW, Blokland KEC, Reid AT, et al., 'Mitochondrial dysfunction contributes to the senescent phenotype of IPF lung fibroblasts', JOURNAL OF CELLULAR AND MOLECULAR MEDICINE, 22 5847-5861 (2018) [C1]
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Nova |
2018 |
Singanayagam A, Glanville N, Girkin JL, Ching YM, Marcellini A, Porter JD, et al., 'Corticosteroid suppression of antiviral immunity increases bacterial loads and mucus production in COPD exacerbations', NATURE COMMUNICATIONS, 9 (2018) [C1]
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Nova |
2018 |
Sarwar G, Bisquera A, Peel R, Hancock S, Grainge C, Attia J, 'The effect of inhaled corticosteroids on bone mineral density measured by quantitative ultrasonography in an older population', Clinical Respiratory Journal, 12 659-665 (2018) [C1]
Introduction: Prolonged use of systemic corticosteroids leads to reduced bone mineral density and osteoporosis, in turn increasing the risk of minimal trauma fractures with their ... [more]
Introduction: Prolonged use of systemic corticosteroids leads to reduced bone mineral density and osteoporosis, in turn increasing the risk of minimal trauma fractures with their associated morbidity and mortality in elderly populations. However, the effect of inhaled corticosteroids on bone mineral density has been debated in the medical literature. Objectives: We aimed to determine the effect of inhaled corticosteroids on bone mineral density measured using calcaneal quantitative ultrasonography in a cohort of older Australians. Methods: Data was collected from the Hunter Community Study, a longitudinal cohort of Australians aged 55-85. Simple and multiple linear regression methods were used to test the cross-sectional association between inhaled corticosteroids and calcaneal bone mineral density measured with quantitative ultrasound at baseline. A causal diagram was used to determine the minimally sufficient number of co-variates necessary to determine the unconfounded effect of inhaled corticosteroids on bone mineral density; these included gender, body mass index, smoking, asthma, alcohol use, age, physical activity, and diet. Results: There were 152 (6.8%) patients on inhaled corticosteroids and 2098 (93%) controls. Simple and multiple linear regression methods showed a non-significant effect of inhaled steroids on BMD with slight decrease of BMD -0.010 g/cm2 (95% CI -0.042 to 0.022, P =.55) and -0.013 g/cm2 (95% CI -0.062 to 0.036, P =.61) respectively. Age, gender, body mass index, and smoking were stronger predictors of BMD. Conclusions: No statistically significant relationship was detected between the use of inhaled corticosteroids and reduced bone mineral density in this observational study of a cohort of older Australians.
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Nova |
2018 |
Jo HE, Prasad JD, Troy LK, Mahar A, Bleasel J, Ellis SJ, et al., 'Diagnosis and management of idiopathic pulmonary fibrosis: Thoracic Society of Australia and New Zealand and Lung Foundation Australia position statements summary', MEDICAL JOURNAL OF AUSTRALIA, 208 82-+ (2018)
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2018 |
Grainge C, Park J-A, 'Inflammatory insights into airway remodelling in asthma', RESPIROLOGY, 23 1084-1085 (2018)
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2018 |
Schuliga M, Grainge C, Westall G, Knight D, 'The fibrogenic actions of the coagulant and plasminogen activation systems in pulmonary fibrosis', International Journal of Biochemistry and Cell Biology, 97 108-117 (2018) [C1]
Fibrosis causes irreversible damage to lung structure and function in restrictive lung diseases such as idiopathic pulmonary fibrosis (IPF). Extravascular coagulation involving fi... [more]
Fibrosis causes irreversible damage to lung structure and function in restrictive lung diseases such as idiopathic pulmonary fibrosis (IPF). Extravascular coagulation involving fibrin formation in the intra-alveolar compartment is postulated to have a pivotal role in the development of pulmonary fibrosis, serving as a provisional matrix for migrating fibroblasts. Furthermore, proteases of the coagulation and plasminogen activation (plasminergic) systems that form and breakdown fibrin respectively directly contribute to pulmonary fibrosis. The coagulants, thrombin and factor Xa (FXa) evoke fibrogenic effects via cleavage of the N-terminus of protease-activated receptors (PARs). Whilst the formation and activity of plasmin, the principle plasminergic mediator is suppressed in the airspaces of patients with IPF, localized increases are likely to occur in the lung interstitium. Plasmin-evoked proteolytic activation of factor XII (FXII), matrix metalloproteases (MMPs) and latent, matrix-bound growth factors such as epidermal growth factor (EGF) indirectly implicate plasmin in pulmonary fibrosis. Another plasminergic protease, urokinase plasminogen activator (uPA) is associated with regions of fibrosis in the remodelled lung of IPF patients and elicits fibrogenic activity via binding its receptor (uPAR). Plasminogen activator inhibitor-1 (PAI-1) formed in the injured alveolar epithelium also contributes to pulmonary fibrosis in a manner that involves vitronectin binding. This review describes the mechanisms by which components of the two systems primarily involved in fibrin homeostasis contribute to interstitial fibrosis, with a particular focus on IPF. Selectively targeting the receptor-mediated mechanisms of coagulant and plasminergic proteases may limit pulmonary fibrosis, without the bleeding complications associated with conventional anti-coagulant and thrombolytic therapies.
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Nova |
2018 |
Grainge CL, Lau LC, Ward JA, Dulay V, Lahiff G, Wilson S, et al., 'Effect of Bronchoconstriction on Airway Remodeling in Asthma (vol 364, pg 2006, 2011)', NEW ENGLAND JOURNAL OF MEDICINE, 378 2450-2450 (2018) |
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2018 |
Jo H, Troy L, Keir G, Chambers D, Holland A, Goh N, et al., 'Treatment of Idiopathic Pulmonary Fibrosis in Australia and New Zealand: A position statement from the Thoracic Society of Australia and New Zealand and the Lung Foundation Australia (vol 22, pg 1436, 2017)', RESPIROLOGY, 23 116-116 (2018)
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2018 |
Jo HE, Glaspole I, Moodley Y, Chapman S, Ellis S, Goh N, et al., 'Disease progression in idiopathic pulmonary fibrosis with mild physiological impairment: Analysis from the Australian IPF registry', BMC Pulmonary Medicine, 18 (2018) [C1]
Background: Idiopathic pulmonary fibrosis (IPF) is a progressive and fatal fibrosing lung disease of unknown cause. The advent of anti-fibrotic medications known to slow disease p... [more]
Background: Idiopathic pulmonary fibrosis (IPF) is a progressive and fatal fibrosing lung disease of unknown cause. The advent of anti-fibrotic medications known to slow disease progression has revolutionised IPF management in recent years. However, little is known about the natural history of IPF patients with mild physiological impairment. We aimed to assess the natural history of these patients using data from the Australian IPF Registry (AIPFR). Methods: Using our cohort of real-world IPF patients, we compared FVC criteria for mild physiological impairment (FVC = 80%) against other proposed criteria: DLco = 55%; CPI =40 and GAP stage 1 with regards agreement in classification and relationship with disease outcomes. Within the mild cohort (FVC = 80%), we also explored markers associated with poorer prognosis at 12 months. Results: Of the 416 AIPFR patients (mean age 70.4 years, 70% male), 216 (52%) were classified as 'mild' using FVC = 80%. There was only modest agreement between FVC and DLco (k = 0.30), with better agreement with GAP (k = 0.50) and CPI (k = 0.48). Patients who were mild had longer survival, regardless of how mild physiologic impairment was defined. There was, however, no difference in the annual decline in FVC% predicted between mild and moderate-severe groups (for all proposed criteria). For patients with mild impairment (n = 216, FVC = 80%), the strongest predictor of outcomes at 12 months was oxygen desaturation on a 6 min walk test. Conclusion: IPF patients with mild physiological impairment have better survival than patients with moderate-severe disease. Their overall rate of disease progression however, is comparable, suggesting that they are simply at different points in the natural history of IPF disease.
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2017 |
Williamson JP, Twaddell SH, Lee YCG, Salamonsen M, Hew M, Fielding D, et al., 'Thoracic ultrasound recognition of competence: A position paper of the Thoracic Society of Australia and New Zealand', Respirology, 22 405-408 (2017) [C1]
The ability to perform bedside thoracic ultrasound is increasingly recognized as an essential skill for thoracic clinicians, extending the clinical examination and aiding diagnost... [more]
The ability to perform bedside thoracic ultrasound is increasingly recognized as an essential skill for thoracic clinicians, extending the clinical examination and aiding diagnostic and therapeutic procedures. Thoracic ultrasound reduces complications and increases success rates when used prior to thoracentesis or intercostal chest tube insertion. It is increasingly difficult to defend performing these procedures without real or near-real time image guidance. To assist thoracic physicians and others achieve and demonstrate thoracic ultrasound competence, the Interventional Pulmonology Special Interest Group (IP-SIG) of the Thoracic Society of Australia and New Zealand (TSANZ) has developed a new pathway with four components: (i) completion of an approved thoracic ultrasound theory and hands-on teaching course. (ii) A log of at least 40 relevant scans. (iii) Two formative assessments (following 5¿10 scans and again after 20 scans) using the Ultrasound-Guided Thoracentesis Skills and Tasks Assessment Tool (UG-STAT). (iv) A barrier assessment (UG-STAT, pass score of 90%) by an accredited assessor not directly involved in the candidate's training. Upon completion of these requirements a candidate may apply to the TSANZ for recognition of competence. This pathway is intended to provide a regional standard for thoracic ultrasound training.
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Nova |
2017 |
Murray LA, Grainge C, Wark PA, Knight DA, 'Use of biologics to treat acute exacerbations and manage disease in asthma, COPD and IPF', Pharmacology and Therapeutics, 169 1-12 (2017) [C1]
A common feature of chronic respiratory disease is the progressive decline in lung function. The decline can be indolent, or it can be accelerated by acute exacerbations, whereby ... [more]
A common feature of chronic respiratory disease is the progressive decline in lung function. The decline can be indolent, or it can be accelerated by acute exacerbations, whereby the patient experiences a pronounced worsening of disease symptoms. Moreover, acute exacerbations may also be a marker of insufficient disease management. The underlying cause of an acute exacerbation can be due to insults such as pathogens or environmental pollutants, or the cause can be unknown. For each acute exacerbation, the patient may require medical intervention such as rescue medication, or in more severe cases, hospitalization and ventilation and have an increased risk of death. Biologics, such as monoclonal antibodies, are being developed for chronic respiratory diseases including asthma, COPD and IPF. This therapeutic approach is particularly well suited for chronic use based on the route and frequency of delivery and importantly, the potential for disease modification. In recent clinical trials, the frequency of acute exacerbation has often been included as an endpoint, to help determine whether the investigational agent is impacting disease. Therefore the significance of acute exacerbations in driving disease, and their potential as a marker of disease activity and progression, has recently received much attention. There is also now a need to standardize the definition of an acute exacerbation in specific disease settings, particularly as this endpoint is increasingly used in clinical trials to also assess therapeutic efficacy. Moreover, specifically targeting exacerbations may offer a new therapeutic approach for several chronic respiratory diseases.
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Nova |
2017 |
Glaspole IN, Watson AL, Allan H, Chapman S, Cooper WA, Corte TJ, et al., 'Determinants and outcomes of prolonged anxiety and depression in idiopathic pulmonary fibrosis', EUROPEAN RESPIRATORY JOURNAL, 50 (2017)
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2017 |
Jo HE, Glaspole I, Grainge C, Goh N, Hopkins PMA, Moodley Y, et al., 'Baseline characteristics of idiopathic pulmonary fibrosis: analysis from the Australian Idiopathic Pulmonary Fibrosis Registry (vol 49, 1601592, 2017)', EUROPEAN RESPIRATORY JOURNAL, 49 (2017)
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2017 |
Schuliga M, Jaffar J, Berhan A, Langenbach S, Harris T, Waters D, et al., 'Annexin A2 contributes to lung injury and fibrosis by augmenting factor Xa fibrogenic activity', American Journal of Physiology - Lung Cellular and Molecular Physiology, 312 L772-L782 (2017) [C1]
In lung injury and disease, including idiopathic pulmonary fibrosis (IPF), extravascular factor X is converted into factor Xa (FXa), a coagulant protease with fibrogenic actions. ... [more]
In lung injury and disease, including idiopathic pulmonary fibrosis (IPF), extravascular factor X is converted into factor Xa (FXa), a coagulant protease with fibrogenic actions. Extracellular annexin A2 binds to FXa, augmenting activation of the protease-activated receptor-1 (PAR-1). In this study, the contribution of annexin A2 in lung injury and fibrosis was investigated. Annexin A2 immunoreactivity was observed in regions of fibrosis, including those associated with fibroblasts in lung tissue of IPF patients. Furthermore, annexin A2 was detected in the conditioned media and an EGTA membrane wash of human lung fibroblast (LF) cultures. Incubation with human plasma (5% vol/vol) or purified FXa (15¿50 nM) evoked fibrogenic responses in LF cultures, with FXa increasing interleukin-6 (IL-6) production and cell number by 270 and 46%, respectively (P < 0.05, n = 5¿8). The fibrogenic actions of plasma or FXa were attenuated by the selective FXa inhibitor apixaban (10 µM, or antibodies raised against annexin A2 or PAR-1 (2 µg/ml). FXastimulated LFs from IPF patients (n = 6) produced twice as much IL-6 as controls (n = 10) (P < 0.05), corresponding with increased levels of extracellular annexin A2. Annexin A2 gene deletion in mice reduced bleomycin-induced increases in bronchoalveolar lavage fluid (BALF) IL-6 levels and cell number (*P < 0.05; n = 4¿12). Lung fibrogenic gene expression and dry weight were reduced by annexin A2 gene deletion, but lung levels of collagen were not. Our data suggest that annexin A2 contributes to lung injury and fibrotic disease by mediating the fibrogenic actions of FXa. Extracellular annexin A2 is a potential target for the treatment of IPF.
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Nova |
2017 |
Jo HE, Glaspole I, Grainge C, Goh N, Hopkins PMA, Moodley Y, et al., 'Baseline characteristics of idiopathic pulmonary fibrosis: analysis from the Australian Idiopathic Pulmonary Fibrosis Registry', EUROPEAN RESPIRATORY JOURNAL, 49 (2017) [C1]
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2017 |
Jo HE, Troy LK, Keir G, Chambers DC, Holland A, Goh N, et al., 'Treatment of idiopathic pulmonary fibrosis in Australia and New Zealand: A position statement from the Thoracic Society of Australia and New Zealand and the Lung Foundation Australia', Respirology, 22 1436-1458 (2017) [C1]
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Nova |
2017 |
Liu G, Cooley MA, Nair PM, Donovan C, Hsu AC, Jarnicki AG, et al., 'Airway remodelling and inflammation in asthma are dependent on the extracellular matrix protein fibulin-1c', JOURNAL OF PATHOLOGY, 243 510-523 (2017) [C1]
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Nova |
2016 |
Grainge C, Thomas PS, Mak JCW, Benton MJ, Lim TK, Ko FWS, 'Year in review 2015: Asthma and chronic obstructive pulmonary disease', RESPIROLOGY, 21 765-775 (2016)
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2016 |
Grainge CL, Maltby S, Gibson PG, Wark PAB, McDonald VM, 'Targeted therapeutics for severe refractory asthma: monoclonal antibodies', EXPERT REVIEW OF CLINICAL PHARMACOLOGY, 9 927-941 (2016) [C1]
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Nova |
2016 |
Mackay RMA, Grainge CL, Lau LC, Barber C, Clark HW, Howarth PH, 'Airway surfactant protein D deficiency in adults with severe asthma', Chest, 149 1165-1172 (2016) [C1]
BACKGROUND: Surfactant protein D (SP-D) is an essential component of the innate immune defense against pathogens within the airways. SP-D also regulates allergic inflammation and ... [more]
BACKGROUND: Surfactant protein D (SP-D) is an essential component of the innate immune defense against pathogens within the airways. SP-D also regulates allergic inflammation and promotes the removal of apoptotic cells. SP-D dysregulation is evident in several pulmonary diseases. Our aim was to investigate whether airway and serum levels of SP-D are altered in treatment-resistant severe asthma. METHODS: SP-D concentrations were measured in matched serum and BAL samples collected from 10 healthy control subjects (HC) and 50 patients with asthma (22 with mild asthma [MA] and 28 with severe asthma [SA]). These samples were also evaluated by using Western blot analysis to investigate variations in SP-D size. RESULTS: SP-D levels in BAL samples were significantly lower in SA compared with HC and MA (P < .001) and inversely correlated with BAL eosinophil cationic protein concentrations in SA (P < .01). Serum SP-D was significantly increased in SA compared with HC and MA (P < .001), and BAL/serum ratios were significantly lower in SA compared with HC and MA (P < .001). Reduced SP-D levels in BAL samples, with concomitant increases in serum in SA, were associated with degraded fragments of SP-D in the serum and increased BAL neutrophil counts and lipopolysaccharide levels. CONCLUSIONS: These findings suggest defective innate immunity within the airways in SA, as reflected by low BAL SP-D concentrations and altered bacterial presence with airway neutrophilia. Furthermore, BAL SP-D leakage into the serum in patients with SA may provide a peripheral blood biomarker, reflecting increased epithelial damage and/or epithelial permeability within the peripheral airways.
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Nova |
2016 |
Hill AR, Donaldson JE, Blume C, Smithers N, Tezera L, Tariq K, et al., 'IL-1 alpha mediates cellular cross-talk in the airway epithelial mesenchymal trophic unit', TISSUE BARRIERS, 4 (2016) [C1]
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Nova |
2016 |
Rupani H, Martinez-Nunez RT, Dennison P, Lau LCK, Jayasekera N, Havelock T, et al., 'Toll-like Receptor 7 Is Reduced in Severe Asthma and Linked to an Altered MicroRNA Profile', AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, 194 26-37 (2016) [C1]
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Nova |
2016 |
Mahmoodi E, Grainge C, Erdstein A, O'kane G, 'Septic arthritis caused by pet rodents: A diagnostic dilemma', AUSTRALASIAN MEDICAL JOURNAL, 9 270-273 (2016)
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2016 |
Mackay R-MA, Grainge CL, Lau LC, Barber C, Clark HW, Howarth PH, 'Serum Surfactant Protein D as a Marker of Asthma Severity Response', CHEST, 150 474-474 (2016)
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2015 |
Gosens R, Grainge C, 'Bronchoconstriction and airway biology: Potential impact and therapeutic opportunities', Chest, 147 798-803 (2015) [C1]
Recent work has demonstrated that mechanical forces occurring in the airway as a consequence of bronchoconstriction are sufficient to not only induce symptoms but also influence a... [more]
Recent work has demonstrated that mechanical forces occurring in the airway as a consequence of bronchoconstriction are sufficient to not only induce symptoms but also influence airway biology. Animal and human in vitro and in vivo work demonstrates that the airways are structurally and functionally altered by mechanical stress induced by bronchoconstriction. Compression of the airway epithelium and mechanosensing by the airway smooth muscle trigger the activation and release of growth factors, causing cell proliferation, extracellular matrix protein accumulation, and goblet cell differentiation. These effects of bronchoconstriction are of major importance to asthma pathophysiology and appear sufficient to induce remodeling independent of the inflammatory response. We review these findings in detail and discuss previous studies in light of this new evidence regarding the influence of mechanical forces in the airways. Furthermore, we highlight potential impacts of therapies influencing mechanical forces on airway structure and function in asthma.
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Nova |
2015 |
Lim TK, Ko FW, Thomas PS, Grainge C, Yang IA, 'Year in review 2014: Chronic obstructive pulmonary disease, asthma and airway biology', Respirology, (2015) [C3]
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2015 |
Sarwar G, de Malmanche T, Rassam L, Grainge C, Williams A, Arnold D, 'Chronic granulomatous disease presenting as refractory pneumonia in late adulthood.', Respirology case reports, 3 54-56 (2015) [C3]
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2015 |
Tayler N, Grainge C, Gove K, Howarth P, Holloway J, 'Clinical assessment of speech correlates well with lung function during induced bronchoconstriction', Primary Care Respiratory Medicine, 25 (2015) [C1]
Clinical assessment of asthma often includes a crude assessment of speech, for example whether the patient can speak in full sentences. To date, this statement, despite appearing ... [more]
Clinical assessment of asthma often includes a crude assessment of speech, for example whether the patient can speak in full sentences. To date, this statement, despite appearing in national asthma guidelines, has not been related to lung function testing in asthma exacerbation. Seven asthmatics underwent a bronchial challenge and were then recorded reading a standardised text for 1 min. The recordings were played to 88 healthcare professionals who were asked to estimate FEV1% predicted. Health care professionals' estimations showed moderate correlation to FEV1% predicted (rho = 0.61 Po0.01). There were no significant differences between professionals grouped by seniority or speciality. Speech can intuitively be estimated by health care professionals with moderate accuracy. This gives an evidence basis for the assessment in speech in acute asthma and may provide a new avenue for monitoring.
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Nova |
2014 |
Grainge C, Dennison P, Lau L, Davies D, Howarth P, 'Asthmatic and Normal Respiratory Epithelial Cells Respond Differently to Mechanical Apical Stress', AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, 190 477-480 (2014) [C1]
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Nova |
2014 |
Green BJ, Wiriyachaiporn S, Grainge C, Rogers GB, Kehagia V, Lau L, et al., 'Potentially Pathogenic Airway Bacteria and Neutrophilic Inflammation in Treatment Resistant Severe Asthma', PLOS ONE, 9 (2014) [C1]
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2013 |
Grainge CL, Davies DE, 'Epithelial Injury and Repair in Airways Diseases', CHEST, 144 1906-1912 (2013) [C1]
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2013 |
Grainge C, Jayasekera N, Dennison P, Rupani H, Kurukulaaratchy R, Howarth P, 'Case series reporting the effectiveness of mycophenolate mofetil in treatment-resistant asthma', EUROPEAN RESPIRATORY JOURNAL, 42 1134-1137 (2013) [C1]
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2013 |
Grainge C, Jayasekera N, Dennison P, Rupani H, Kurukulaaratchy R, Howarth P, 'MYCOPHENOLATE MOFETIL IMPROVES LUNG FUNCTION AND SYMPTOMS IN SEVERE TREATMENT RESISTANT ASTHMA', RESPIROLOGY, 18 12-12 (2013) [E3]
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Nova |
2012 |
Grainge C, Dulay V, Ward J, Lau L, Cottey L, Haitchi HM, et al., 'RESISTIN-LIKE MOLECULE BETA IN BRONCHIAL EPITHELIUM INCREASES WITH ASTHMA SEVERITY AND AIRWAY CHALLENGES', RESPIROLOGY, 17 26-26 (2012) [E3] |
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Nova |
2012 |
Grainge C, Dennison P, Davies DE, Howarth PH, 'COMPRESSION STIMULATES TGF beta 2 RELEASE FROM ASTHMATIC BUT NOT NORMAL PRIMARY BRONCHIAL EPITHELIAL CELLS', RESPIROLOGY, 17 42-42 (2012) [E3] |
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Nova |
2012 |
Grainge C, Dragolea N, Howarth PH, 'ALLERGEN CHALLENGE INCREASES ALTERNATIVELY ACTIVATED MACROPHAGES WITHIN THE AIRWAYS IN ALLERGIC ASTHMA', RESPIROLOGY, 17 51-51 (2012) [E3] |
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Nova |
2012 |
Grainge C, Dulay V, Ward J, Sammut D, Davies E, Green B, et al., 'Resistin-like molecule-beta is induced following bronchoconstriction of asthmatic airways', RESPIROLOGY, 17 1094-1100 (2012) [C1]
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Nova |
2011 |
Grainge C, Howarth PH, 'Repeated high-dose inhalation allergen challenge in asthma', CLINICAL RESPIRATORY JOURNAL, 5 150-155 (2011) [C1]
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Nova |
2011 |
Grainge CL, Lau LCK, Ward JA, Dulay V, Lahiff G, Wilson S, et al., 'Effect of Bronchoconstriction on Airway Remodeling in Asthma', NEW ENGLAND JOURNAL OF MEDICINE, 364 2006-2015 (2011) [C1]
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2011 |
Grainge CL, Howarth PH, 'Bronchoconstriction and Airway Remodeling REPLY', NEW ENGLAND JOURNAL OF MEDICINE, 365 1157-1157 (2011)
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Nova |
2011 |
Grainge CL, Howarth PH, 'The authors reply', New England Journal of Medicine, 365 1157 (2011) |
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Nova |
2011 |
Cakebread JA, Xu Y, Grainge C, Kehagia V, Howarth PH, Holgate ST, Davies DE, 'Exogenous IFN-beta has antiviral and anti-inflammatory properties in primary bronchial epithelial cells from asthmatic subjects exposed to rhinovirus', JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, 127 1148-U416 (2011) [C1]
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Nova |
2010 |
Grainge C, Jugg BJ, Smith AJ, Brown RFR, Jenner J, Parkhouse DA, Rice P, 'Delayed low-dose supplemental oxygen improves survival following phosgene-induced acute lung injury', INHALATION TOXICOLOGY, 22 552-560 (2010)
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Nova |
2010 |
Grainge C, Rice P, 'Management of phosgene-induced acute lung injury', CLINICAL TOXICOLOGY, 48 497-508 (2010) [D1]
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Nova |
2010 |
Hatzivlassiou M, Grainge C, Kehagia V, Lau L, Howarth PH, 'The allergen specificity of the late asthmatic reaction', ALLERGY, 65 355-358 (2010)
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Nova |
2010 |
Grainge C, Smith AJ, Jugg BJ, Fairhall SJ, Mann T, Perrott R, et al., 'Furosemide in the treatment of phosgene induced acute lung injury.', Journal of the Royal Army Medical Corps, 156 245-250 (2010)
Using previously validated methods, 16 anaesthetised large white pigs were exposed to phosgene (target inhaled dose 0.3 mg kg(-1)), established on mechanical ventilation and rando... [more]
Using previously validated methods, 16 anaesthetised large white pigs were exposed to phosgene (target inhaled dose 0.3 mg kg(-1)), established on mechanical ventilation and randomised to treatment with either nebulised furosemide (4 ml of 10 mg x ml(-1) solution) or saline control. Treatments were given at 1, 3, 5, 7, 9, 12, 16 and 20 hours post phosgene exposure; the animals were monitored to 24 hours following phosgene exposure. Furosemide treatment had no effect on survival, and had a deleterious effect on PaO2: FiO2 ratio between 19 and 24 hours. All other measures investigated were unaffected by treatment. Nebulised furosemide treatment following phosgene induced acute lung injury does not improve survival and worsens PaO2: FiO2 ratio. Nebulised furosemide should be avoided following phosgene exposure.
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2009 |
Grainge C, Brown R, Jugg BJ, Smith AJ, Mann TM, Jenner J, et al., 'Early treatment with nebulised salbutamol worsens physiological measures and does not improve survival following phosgene induced acute lung injury.', Journal of the Royal Army Medical Corps, 155 105-109 (2009)
To examine the effectiveness of nebulised salbutamol in the treatment of phosgene induced acute lung injury. Using previously validated methods, 12 anaesthetised large white pigs ... [more]
To examine the effectiveness of nebulised salbutamol in the treatment of phosgene induced acute lung injury. Using previously validated methods, 12 anaesthetised large white pigs were exposed to phosgene (Ct 1978 +/- 8 mg min m(-3)), established on mechanical ventilation and randomised to treatment with either nebulised salbutamol (2.5 mg per dose) or saline control. Treatments were given 1, 5, 9, 13, 17 and 21 hours following phosgene exposure. The animals were followed to 24 hours following phosgene exposure. Salbutamol treatment had no effect on mortality and had a deleterious effect on arterial oxygenation, shunt fraction and heart rate. There was a reduction in the number of neutrophils from 24.0% +/- 4.4 to 12.17% +/- 2.1 (p < 0.05) in bronchoalveolar lavage, with some small decreases in inflammatory mediators in bronchoalveolar lavage but not in plasma. Nebulised salbutamol treatment following phosgene induced acute lung injury does not improve survival, and worsens various physiological parameters including arterial oxygen partial pressure and shunt fraction. Salbutamol treatment reduces neutrophil influx into the lung. Its sole use following phosgene exposure is not recommended.
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2005 |
Grainge C, Traer E, Fulton J, 'Do weekend plan standard forms improve communication and influence quality of patient care?', POSTGRADUATE MEDICAL JOURNAL, 81 524-525 (2005)
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2005 |
Grainge C, Nokes T, 'Cerebral arterial thrombosis in a young woman following vasopressin for von Willebrand's disease', THROMBOSIS AND HAEMOSTASIS, 93 380-380 (2005)
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2005 |
Grainge C, Heber M, 'The role of the physician in modern military operations: 12 months experience in Southern Iraq.', Journal of the Royal Army Medical Corps, 151 101-104 (2005)
OBJECTIVES: To examine the profile of medical morbidity and the role of the physician in modern conflict. METHODS: Retrospective survey of admission records at a British Military ... [more]
OBJECTIVES: To examine the profile of medical morbidity and the role of the physician in modern conflict. METHODS: Retrospective survey of admission records at a British Military Field Hospital on operational duty in Southern Iraq. RESULTS: 62.5% of 4870 admissions to the Field Hospital in Shaibah during the first 12 months of military operations in Iraq were under the care of physicians. Of these 1531 (31.4%) were due to diarrhoea and vomiting (D&V) and 764 (15.7%) due to heat illness. The incidence of heat illness rose with ambient temperature, but soldiers were more likely to be admitted with heat illness shortly after arrival in theatre than when fully acclimatised. There was also a steady flow of admissions with a broad spectrum of medical pathology requiring the clinical skills of a general physician. CONCLUSIONS: A general physician is a necessary part of the clinical team in modern conflict. The incidence of D&V and of heat illness on military operations remains high. Planners for any operation in tropical climates should take this into consideration and put preventative measures into place early.
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2004 |
Grainge C, 'Breath of life: the evolution of oxygen therapy', JOURNAL OF THE ROYAL SOCIETY OF MEDICINE, 97 489-493 (2004)
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2001 |
Blanco G, Coulton GR, Biggin A, Grainge C, Moss J, Barrett M, et al., 'The kyphoscoliosis (ky) mouse is deficient in hypertrophic responses and is caused by a mutation in a novel muscle-specific protein', HUMAN MOLECULAR GENETICS, 10 9-16 (2001)
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