Dr Kirsten Coupland

Dr Kirsten Coupland

Postdoctoral Researcher

School of Biomedical Sciences and Pharmacy (Human Physiology)

Career Summary

Biography

Dr. Kirsten Coupland is a postdoctoral researcher based at the Hunter Medical Research Institute (HMRI) and the University of Newcastle. Her current research examines the molecular basis of post-stroke outcomes, the results of which she aims to see implemented in a clinical setting.

Dr. Coupland was awarded her PhD from the University of New South Wales in 2015 for her work examining the expression of the microtubule associated protein tau (MAPT) gene in late-onset neurodegenerative disease and its association with disease risk and modifiable lifestyle factors. Under the supervision of Dr. Carol Dobson-Stone and A/Prof. John Kwok, Kirsten’s work identified multiple genetic factors with the potential to contribute to neurodegenerative disease risk. Her work led to the finding that increased DNA methylation (a modifiable epigenetic mark) of MAPT was associated with delayed onset of Parkinson’s disease, and that this methylation was modifiable by vitamin E. These findings led to two awards and an invitation by the Royal Society to present at the Commonwealth Science Conference in Bangalore, India.

Following her PhD, Kirsten took up a postdoctoral position at Karolsinka Institutet, Sweden in the lab of A/Prof Helena Karlström to examine the molecular underpinnings of an inherited vascular dementia (CADASIL). This condition arises due to mutations in a single gene (NOTCH3) and manifests as a series of small strokes that damage the brain and lead to memory impairment. This work focused on the development of a therapy that directs the immune system to remove a toxic build-up of protein in order to pre-emptively treat CADASIL. Her research at Karolinska Institutet resulted in the securing of several competitive grants that continue to fund this important work. In recognition of her skills and experience in neurodegenerative disease and vasculature, Kirsten was recruited to the labs of Prof. Neil Spratt and A/Prof Rohan Walker in May 2018 under the prestigious Dalara Foundation fellowship where she will investigate new targets for post-stroke therapy to improve patient outcomes.


Qualifications

  • Doctor of Philosophy, University of New South Wales
  • Bachelor of Science (Honours), University of New South Wales
  • Diploma in Innovation Management, University of New South Wales

Keywords

  • Stroke
  • Glymphatics
  • Cerebrovasculature
  • Neuroscience
  • Neurodegeneration
  • Epigenetics

Languages

  • English (Mother)

Fields of Research

Code Description Percentage
110999 Neurosciences not elsewhere classified 100

Professional Experience

UON Appointment

Title Organisation / Department
Postdoctoral Researcher University of Newcastle
School of Biomedical Sciences and Pharmacy
Australia

Academic appointment

Dates Title Organisation / Department
16/02/2015 - 27/04/2018 Postdoctoral Researcher Karolinska Institute
Neurobiology, Care Science and Society
Sweden
1/03/2010 - 23/12/2011 Research Assistant NeuRA
Ageing and Neurodegeneration
Australia

Awards

Prize

Year Award
2012 Junior Oral Presentation Prize
Prince of Wales Childrens Hospital
2012 Student Oral Presentation
Australasian GeneMappers Conference

Invitations

Participant

Year Title / Rationale
2015 Synthetic Biology Summer School

Speaker

Year Title / Rationale
2014 Commonwealth Science Conference
2013 Amplify Festival Brightsparks

Teaching

Code Course Role Duration
0000 Erasmus Internship
Karolinska Institute
In-lab supervisor 13/04/2015 - 14/09/2015
0000 Erasmus internship
Karolinska Institute
In-lab supervisor 4/09/2017 - 4/12/2017
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Publications

For publications that are currently unpublished or in-press, details are shown in italics.


Journal article (6 outputs)

Year Citation Altmetrics Link
2018 Panahi M, Yousefi Mesri N, Samuelsson EB, Coupland KG, Forsell C, Graff C, et al., 'Differences in proliferation rate between CADASIL and control vascular smooth muscle cells are related to increased TGFß expression', Journal of Cellular and Molecular Medicine, 22 3016-3024 (2018)

© 2018 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine. Cerebral autosomal-domi... [more]

© 2018 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine. Cerebral autosomal-dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL) is a familial fatal progressive degenerative disorder. One of the pathological hallmarks of CADASIL is a dramatic reduction of vascular smooth muscle cells (VSMCs) in cerebral arteries. Using VSMCs from the vasculature of the human umbilical cord, placenta and cerebrum of CADASIL patients, we found that CADASIL VSMCs had a lower proliferation rate compared to control VSMCs. Exposure of control VSMCs and endothelial cells (ECs) to media derived from CADASIL VSMCs lowered the proliferation rate of all cells examined. By quantitative RT-PCR analysis, we observed increased Transforming growth factor-ß (TGFß) gene expression in CADASIL VSMCs. Adding TGFß-neutralizing antibody restored the proliferation rate of CADASIL VSMCs. We assessed proliferation differences in the presence or absence of TGFß-neutralizing antibody in ECs co-cultured with VSMCs. ECs co-cultured with CADASIL VSMCs exhibited a lower proliferation rate than those co-cultured with control VSMCs, and neutralization of TGFß normalized the proliferation rate of ECs co-cultured with CADASIL VSMCs. We suggest that increased TGFß expression in CADASIL VSMCs is involved in the reduced VSMC proliferation in CADASIL and may play a role in situ in altered proliferation of neighbouring cells in the vasculature.

DOI 10.1111/jcmm.13534
2016 Coupland KG, Kim WS, Halliday GM, Hallupp M, Dobson-Stone C, Kwok JBJ, 'Role of the Long Non-Coding RNA MAPT-AS1 in Regulation of Microtubule Associated Protein Tau (MAPT) Expression in Parkinson's Disease', PLOS ONE, 11 (2016)
DOI 10.1371/journal.pone.0157924
Citations Scopus - 3Web of Science - 3
2015 Coupland KG, Kim WS, Halliday GM, Hallupp M, Dobson-Stone C, Kwok JBJ, 'Effect of PSEN1 Mutations on MAPT Methylation in Early-Onset Alzheimer's Disease', CURRENT ALZHEIMER RESEARCH, 12 745-751 (2015)
DOI 10.2174/1567205012666150710110756
Citations Scopus - 3Web of Science - 3
2014 Coupland KG, Mellick GD, Silburn PA, Mather K, Armstrong NJ, Sachdev PS, et al., 'DNA Methylation of the MAPT Gene in Parkinson's Disease Cohorts and Modulation by Vitamin E In Vitro', MOVEMENT DISORDERS, 29 1606-1614 (2014)
DOI 10.1002/mds.25784
Citations Scopus - 29Web of Science - 27
2012 Zemb O, Lee M, Gutierrez-Zamora ML, Hamelin J, Coupland K, Hazrin-Chong NH, et al., 'Improvement of RNA-SIP by pyrosequencing to identify putative 4-n-nonylphenol degraders in activated sludge', WATER RESEARCH, 46 601-610 (2012)
DOI 10.1016/j.watres.2011.10.047
Citations Scopus - 15Web of Science - 13
2010 Luty AA, Kwok JBJ, Dobson-Stone C, Loy CT, Coupland KG, Karlstrom H, et al., 'Sigma Nonopioid Intracellular Receptor 1 Mutations Cause Frontotemporal Lobar Degeneration-Motor Neuron Disease', ANNALS OF NEUROLOGY, 68 639-649 (2010)
DOI 10.1002/ana.22274
Citations Scopus - 102Web of Science - 97
Show 3 more journal articles

Conference (1 outputs)

Year Citation Altmetrics Link
2015 Kwok J, Coupland K, Mellick G, Mather K, Thalamuthu A, Armstrong N, et al., 'Genome-wide association study identifies the SLC2A14 gene on chromosome 12P13 as a trans-acting locus for methylation of MAPT gene', JOURNAL OF NEUROCHEMISTRY, Cairns, AUSTRALIA (2015)
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Grants and Funding

Summary

Number of grants 11
Total funding $804,800

Click on a grant title below to expand the full details for that specific grant.


20174 grants / $761,500

HMRI Early Career Research Fellowship in Stroke, supported by Dalara Foundation$712,500

Funding body: Dalara Foundation

Funding body Dalara Foundation
Project Team Professor Neil Spratt, Doctor Kirsten Coupland
Scheme Research Funding
Role Investigator
Funding Start 2017
Funding Finish 2022
GNo G1701347
Type Of Funding C3120 - Aust Philanthropy
Category 3120
UON Y

Institutional grant$21,000

Funding body: Karolinska Institutet Fonden Geriatrik

Funding body Karolinska Institutet Fonden Geriatrik
Scheme Karolinska Institutet Fonden Geriatrik
Role Lead
Funding Start 2017
Funding Finish 2018
GNo
Type Of Funding International - Competitive
Category 3IFA
UON N

Research Grant$17,000

Funding body: Loo och Hans Ostermans

Funding body Loo och Hans Ostermans
Scheme Loo och Hans Ostermans
Role Lead
Funding Start 2017
Funding Finish 2018
GNo
Type Of Funding International - Competitive
Category 3IFA
UON N

Foundation Grant$11,000

Funding body: Gamla Tjänarinnor

Funding body Gamla Tjänarinnor
Scheme Gamla Tjänarinnor
Role Lead
Funding Start 2017
Funding Finish 2018
GNo
Type Of Funding International - Competitive
Category 3IFA
UON N

20164 grants / $20,000

Institutional grant$8,300

Funding body: Karolinska Institutet Fonden Geriatrik

Funding body Karolinska Institutet Fonden Geriatrik
Scheme Karolinska Institutet Fonden Geriatrik
Role Lead
Funding Start 2016
Funding Finish 2017
GNo
Type Of Funding International - Competitive
Category 3IFA
UON N

Foundation Grant$5,000

Funding body: Gamla Tjänarinnor

Funding body Gamla Tjänarinnor
Scheme Gamla Tjänarinnor
Role Lead
Funding Start 2016
Funding Finish 2017
GNo
Type Of Funding International - Competitive
Category 3IFA
UON N

Research Grant$4,200

Funding body: Strokeförbundet

Funding body Strokeförbundet
Scheme Strokeförbundet
Role Lead
Funding Start 2016
Funding Finish 2017
GNo
Type Of Funding International - Competitive
Category 3IFA
UON N

Research Grant$2,500

Funding body: Stohnes Stiftelse

Funding body Stohnes Stiftelse
Scheme Stohnes Stiftelse
Role Lead
Funding Start 2016
Funding Finish 2017
GNo
Type Of Funding International - Competitive
Category 3IFA
UON N

20153 grants / $23,300

Institutional grant$15,000

Funding body: Karolinska Institutet Fonden Geriatrik

Funding body Karolinska Institutet Fonden Geriatrik
Scheme Karolinska Institutet Fonden Geriatrik
Role Lead
Funding Start 2015
Funding Finish 2016
GNo
Type Of Funding International - Competitive
Category 3IFA
UON N

Foundation Grant$6,700

Funding body: Gamla Tjänarinnor

Funding body Gamla Tjänarinnor
Scheme Gamla Tjänarinnor
Role Lead
Funding Start 2015
Funding Finish 2016
GNo
Type Of Funding International - Competitive
Category 3IFA
UON N

Research Grant$1,600

Funding body: Stohnes Stiftelse

Funding body Stohnes Stiftelse
Scheme Stohnes Stiftelse
Role Lead
Funding Start 2015
Funding Finish 2016
GNo
Type Of Funding International - Non Competitive
Category 3IFB
UON N
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Research Collaborations

The map is a representation of a researchers co-authorship with collaborators across the globe. The map displays the number of publications against a country, where there is at least one co-author based in that country. Data is sourced from the University of Newcastle research publication management system (NURO) and may not fully represent the authors complete body of work.

Country Count of Publications
Australia 6
Sweden 2
United States 2
Finland 1
Poland 1
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Dr Kirsten Coupland

Position

Postdoctoral Researcher
Spratt Lab
School of Biomedical Sciences and Pharmacy
Faculty of Health and Medicine

Focus area

Human Physiology

Contact Details

Email kirsten.coupland@newcastle.edu.au
Phone (02) 4921 16903
Mobile +61 402 180 915

Office

Room MS-502
Building Medical Sciences Building
Location Callaghan
University Drive
Callaghan, NSW 2308
Australia
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