Applications and Capabilities

Q-ToF

Bruker Daltonics’ micrOTOF-Q II is configured with an Apollo ion source followed by an ion transfer stage which guides the ions into an analytical quadrupole and collision cell for accumulation of parent/fragment ions prior to mass analysis in the Time-of-Flight mass spectrometer.

 

As with the LTQ the micrOTOF-Q II is connected to a split-less Nano-Flow Liquid Chromatography system for Reversed Phase pre-separation of sample to reduce complexity when analysed by the Mass Spectrometer. The ion source is generally configured for Nano-Flow, Electro-Spray Ionisation (ESI) MS, however options are available for both Micro-Flow ESI and Atmospheric Pressure Chemical Ionisation (APCI). Detection is possible in both positive and negative ion modes. A syringe pump is also available for direct injection of sample into either the Micro-Flow ESI or APCI ion sources.

The Q-ToF offers an increase in resolution and mass accuracy over the LTQ, yet unlike the ion trap, does not possess the varied options for ion fragmentation and is not capable of performing MSn analysis beyond MS3 (Pseudo MS3 possible with In Source Decay).

 

With mass resolution of better than 17,500 and mass accuracy of 1-2 ppm the new micrOTOF-Q II is a high-performance system for many analytical challenges, including identification and quantitation in LC/MS/MS proteomics, metabolomics and other small molecule applications including highly confident formula determination of small molecules (up to 1kDa) using Bruker’s SmartFormula.

 

 

Bruker Daltonics’ micrOTOF-Q II