Currently Approved SOPs
Current
Standard Operating Procedures - currently approved
The SOPs listed below are currently approved by the ACEC and may be cited in the relevant section of the ACEC application by full title (Number, date of approval and procedure name).
These currently approved SOPs do not need to be attached to an ACEC application.
Please note: ANY variation in technique from an approved SOP as written must be stated in the application and the variation described in full. Unless otherwise stated in the application, the ACEC will assume that the procedure will be conducted in accordance with the SOP and any variation to the SOP without prior approval from the ACEC will constitute a breach of the approved protocol.
SOPs are in numerical order under the appropriate category.
Euthanasia Techniques
- SOP#9 - Sodium pentobarbitone euthanasia - mouse (PDF 120 KB)
- SOP#10 - Euthanasia by cervical dislocation- Mouse (PDF 152 KB)
- SOP#74 - Euthanasia using Carbon Dioxide Gas- rodents and small birds (PDF 124 KB)
- SOP#75 - Euthanasia by intraperitoneal sodium pentobarbitone - rodents (PDF 143 KB)
- SOP#76 - Euthanasia by intravenous sodium pentobarbitone - all species (PDF 141 KB)
- SOP#92 - Euthanasia of frogs and tadpoles (PDF 139 KB)
- SOP#143 - Euthanasia by decapitation - neonatal rats and mice (PDF 162 KB)
- SOP#148 - CO2 asphyxiation - euthanasia dam, foetal and neonatal - guinea pig (PDF 238 KB)
Administration of Substances Techniques
- SOP#77 - Injection, intramuscular- rodents, rabbits and birds (PDF 238 KB)
- SOP#78 - Injection, intraperitoneal- rodents (PDF 238 KB)
- SOP#79 - Injection, intravenous- rodents and birds (PDF 253 KB)
- SOP#80 - Injection, subcutaneous- rodents and rabbits (PDF 126 KB)
- SOP#119 - Oral Gavage - Mouse (PDF 149 KB)
Sampling Techniques (Tissues/Body Fluids/Blood Collection)
- SOP#24 - Blood collection - tail vein rat (PDF 178 KB)
- SOP#39 - Blood collection – tail vein - Mouse (PDF 141 KB)
- SOP#73 - Blood Collection, saphenous vein - mouse (PDF 234 KB)
- SOP#121 - Blood collection- saphenous vein - Rat (PDF 216 KB)
Anaesthesia Techniques
- SOP#6 - Isoflurane anaesthesia of the rat and mouse (PDF 245 KB)
- SOP#7 - Intraperitonal anaesthesia with ketamine/xylazine - Mouse (PDF 154 KB)
- SOP#8 - IV Alfaxan Mouse- non painful procedures (PDF 132 KB)
- SOP#43 - IP Anaesthesia: Ketamine /xylazine or medetomidine-NR - mouse (PDF 245 KB)
- SOP#207 - Anaesthesia- Ketamine, medetomidine - mouse (PDF 132 KB)
Testing Techniques
Respiratory System Techniques
- SOP#19 - Intranasal inoculation - Mouse (PDF 118 KB)
- SOP#114 - Intratracheal inoculation- mouse (PDF 126 KB)
Reproductive Techniques
The University of Newcastle acknowledges the traditional custodians of the lands within our footprint areas: Awabakal, Darkinjung, Biripai, Worimi, Wonnarua, and Eora Nations. We also pay respect to the wisdom of our Elders past and present.