2024 |
Paul N, Maiti K, Sultana Z, Fisher JJ, Zhang H, Cole N, et al., 'Human placenta releases extracellular vesicles carrying corticotrophin releasing hormone mRNA into the maternal blood', Placenta, 146 71-78 (2024) [C1]
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Nova |
2023 |
Paul N, Sultana Z, Fisher JJ, Maiti K, Smith R, 'Extracellular vesicles- crucial players in human pregnancy.', Placenta, 140 30-38 (2023) [C1]
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Nova |
2023 |
Grace T, Fisher J, Wang C, Valkenborghs SR, Smith R, Hirst JJ, et al., 'Newcastle 1000 (NEW1000) Study: an Australian population-based prospective pregnancy cohort study design and protocol', BMJ OPEN, 13 (2023)
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2023 |
Naghipour S, Fisher JJ, Perkins AV, Peart JN, Headrick JP, Toit EFD, 'A gut microbiome metabolite paradoxically depresses contractile function while activating mitochondrial respiration', Disease Models & Mechanisms, 16 (2023) [C1]
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Nova |
2023 |
Foteva V, Fisher JJ, Wyrwoll CS, '"Well, what can I do?": An examination of the role supervisors, peers and scientific societies can play in the multicultural student experience', PLACENTA, 141 65-70 (2023) [C1]
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Nova |
2023 |
Schofield LG, Kahl RGS, Rodrigues SL, Fisher JJ, Endacott SK, Delforce SJ, et al., 'Placental deficiency of the (pro)renin receptor ((P)RR) reduces placental development and functional capacity.', Front Cell Dev Biol, 11 1212898 (2023) [C1]
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Nova |
2023 |
Foteva V, Fisher JJ, Qiao Y, Smith R, 'Does the Micronutrient Molybdenum Have a Role in Gestational Complications and Placental Health?', Nutrients, 15 3348-3348 [C1]
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Nova |
2022 |
Bartho LA, Fisher JJ, Walton SL, Perkins A, Cuffe JSM, 'The effect of gestational age on mitochondrial properties of the mouse placenta', REPRODUCTION AND FERTILITY, 3 19-29 (2022) [C1]
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Nova |
2022 |
Quinn KM, Roberts L, Cox AJ, Borg DN, Pennell EN, McKeating DR, et al., 'Blood oxidative stress biomarkers in women: influence of oral contraception, exercise, and N-acetylcysteine', European Journal of Applied Physiology, 122 1949-1964 (2022) [C1]
Purpose: To compare physiological responses to submaximal cycling and sprint cycling performance in women using oral contraceptives (WomenOC) and naturally cycling women (WomenNC)... [more]
Purpose: To compare physiological responses to submaximal cycling and sprint cycling performance in women using oral contraceptives (WomenOC) and naturally cycling women (WomenNC) and to determine whether N-acetylcysteine (NAC) supplementation mediates these responses. Methods: Twenty recreationally trained women completed five exercise trials (i.e., an incremental cycling test, a familiarisation trial, a baseline performance trial and two double-blind crossover intervention trials). During the intervention trials participants supplemented with NAC or a placebo 1 h before exercise. Cardiopulmonary parameters and blood biochemistry were assessed during 40 min of fixed-intensity cycling at 105% of gas-exchange threshold and after 1-km cycling time-trial. Results: WomenOC had higher ventilation (ß [95% CI] = 0.07 L·min-1 [0.01, 0.14]), malondialdehydes (ß = 12.00¿mmol·L-1 [6.82, 17.17]) and C-reactive protein (1.53¿mg·L-1 [0.76, 2.30]), whereas glutathione peroxidase was lower (ß = 22.62¿mU·mL-1 [-¿41.32, -¿3.91]) compared to WomenNC during fixed-intensity cycling. Plasma thiols were higher at all timepoints after NAC ingestion compared to placebo, irrespective of group (all p < 0.001; d = 1.45 to 2.34). For WomenNC but not WomenOC, the exercise-induced increase in malondialdehyde observed in the placebo trial was blunted after NAC ingestion, with lower values at 40¿min (p = 0.018; d = 0.73). NAC did not affect cycling time-trial performance. Conclusions: Blood biomarkers relating to oxidative stress and inflammation are elevated in WomenOC during exercise. There may be an increased strain on the endogenous antioxidant system during exercise, since NAC supplementation in WomenOC did not dampen the exercise-induced increase in malondialdehyde. Future investigations should explore the impact of elevated oxidative stress on exercise adaptations or recovery from exercise in WomenOC.
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2022 |
Fisher JJ, James JL, 'Know the game: Insights to help early career researchers successfully navigate academia', Placenta, 125 78-83 (2022) [C1]
Career trajectories in science are often unpredictable, with many early and mid-career researchers working multiple successive fixed-term contracts, and physically relocating to t... [more]
Career trajectories in science are often unpredictable, with many early and mid-career researchers working multiple successive fixed-term contracts, and physically relocating to take up employment opportunities. Whilst this can provide exciting opportunities to change research direction, acquire new skills, and see the world, the precarity of this scenario is also a significant cause of anxiety for many, and can have a negative impact on their ability to maintain career momentum and trajectory, access institutional financial benefits, or make long term career or financial plans. Here, we build on a pair of workshops held at the 2021 International Federation of Placenta Associations annual conference to discuss two key areas important to help early career researchers navigate their careers ¿ building an academic profile, and the financial ramifications of academic careers.
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Nova |
2021 |
Bartho LA, O'Callaghan JL, Fisher JJ, Cuffe JSM, Kaitu'u-Lino TJ, Hannan NJ, et al., 'Analysis of mitochondrial regulatory transcripts in publicly available datasets with validation in placentae from pre-term, post-term and fetal growth restriction pregnancies', Placenta, 112 162-171 (2021) [C1]
Introduction: The human placenta has a defined lifespan and placental aging is a key feature as pregnancy progresses. Placental aging and mitochondrial dysfunction are known to pl... [more]
Introduction: The human placenta has a defined lifespan and placental aging is a key feature as pregnancy progresses. Placental aging and mitochondrial dysfunction are known to play a key role in pregnancy pathophysiology. Premature aging of the placenta has also been linked with placental dysfunction resulting in poor fetal development and premature birth. Methods: The expression of key mitochondrial-related genes were analysed in a series of publicly available databases then expression changes were validated in placental samples collected from term, pre-term, post-term pregnancies and pregnancies complicated by fetal growth restriction (FGR). Gene and protein expression levels of MFN1, MFN2, TFAM, TOMM20, OPA3 and SIRT4 were measured in placental tissues via qPCR and western blotting. Results: Initial analysis found that key mitochondrial transcripts related to biogenesis, bioenergetics and mitophagy clustered by pregnancy trimester. A refined list of 13 mitochondrial-related genes were investigated in additional external datasets of pregnancy complications. In the new cohort, protein expression of MFN1 was decreased in FGR and MFN2 is decreased in post-term placenta. Analysis of placental tissues revealed that TOMM20 gene and protein expression was altered in FGR and post-term placenta. Discussion: MFN1 and MFN2 play a major role in mitochondrial dynamics, and alterations in these markers have been highlighted in early unexplained miscarriage. TOMM20 is an importer protein that plays a major role in mitophagy and changes have also been identified in age-related diseases. Significant changes in MFN1, MFN2 and TOMM20 indicate that mitochondrial regulators play a critical role in placental aging and placental pathophysiology.
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Nova |
2021 |
Quinn KM, Cox AJ, Roberts L, Pennell EN, McKeating DR, Fisher JJ, et al., 'Temporal changes in blood oxidative stress biomarkers across the menstrual cycle and with oral contraceptive use in active women', European Journal of Applied Physiology, 121 2607-2620 (2021) [C1]
Purpose: To examine the temporal changes in blood oxidative stress biomarkers in recreationally-trained women that were naturally-cycling (WomenNC) or using oral contraceptives (W... [more]
Purpose: To examine the temporal changes in blood oxidative stress biomarkers in recreationally-trained women that were naturally-cycling (WomenNC) or using oral contraceptives (WomenOC) across one month. Methods: Blood samples were acquired at three timepoints of the menstrual cycle (1: early-follicular, 2: late-follicular and 3: mid-luteal) and oral contraceptive packet (1: InactiveOC, 2: Mid-activeOC and 3: Late-activeOC) for determination of estradiol, progesterone, oxidative stress, C-reactive protein (CRP) and other cardiometabolic biomarkers in plasma and serum. Results: There was a Group by Time effect on estradiol (p < 0.001, partial ¿2 = 0.64) and progesterone (p < 0.001, partial ¿2 = 0.77). Malondialdehyde, lipid hydroperoxides and CRP concentrations were higher in WomenOC during Late-activeOC compared to InactiveOC (+ 96%, + 23% and + 104%, respectively, p < 0.05). However, there were no changes in these biomarkers across the menstrual cycle in WomenNC (p > 0.05). At all timepoints (i.e., 1, 2 and 3), WomenOC had elevated lipid hydroperoxides (+ 28, + 48% and + 50%) and CRP (+ 71%, + 117% and + 130%) compared to WomenNC (p < 0.05, partial ¿2 > 0.25). There was no Group by Time effect on non-enzymatic antioxidants or glutathione peroxidase; however, glutathione peroxidase was lower in WomenOC, i.e., main effect of group (p < 0.05, partial ¿2 > 0.20). Conclusion: These findings demonstrate that WomenOC not only have higher oxidative stress and CRP than WomenNC, but also a transient increase across one month of habitual oral contraceptive use. Since changes in oxidative stress and CRP often relate to training stress and recovery, these outcomes may have implications to workload monitoring practices in female athletes.
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2021 |
McKeating DR, Fisher JJ, MacDonald T, Walker S, Tong S, Bennett WW, et al., 'Circulating trace elements for the prediction of preeclampsia and small for gestational age babies', Metabolomics, 17 (2021) [C1]
Introduction: Poor gestational outcomes due to placental insufficiency can have lifelong consequences for mother and child. Objective: There is a need for better methods of diagno... [more]
Introduction: Poor gestational outcomes due to placental insufficiency can have lifelong consequences for mother and child. Objective: There is a need for better methods of diagnosis, and elemental metabolomics may provide a means to determine the risk of gestational disorders. Methods: This study used blood plasma samples collected at 36¿weeks¿ gestation from women who later developed preeclampsia (n = 38), or small-for-gestational age babies (n = 91), along with matched controls (n = 193). Multi-element analysis was conducted by inductively coupled plasma mass spectrometer (ICP-MS), allowing simultaneous measurement of 28 elements. Results: Women who later developed PE, exhibited significantly increased concentrations of K, Rb and Ba. For SGA pregnancies, there was a significant increase in Cu and a decrease in As concentrations. Despite significant differences in single elements, the elemental profile of groups indicated no clustering of control, PE, or SGA samples. Positive predicative values correctly identified approximately 60% of SGA and 70% of PE samples. Conclusion: This is the first-time elemental metabolomics has been used to predict SGA and PE at 36¿weeks. Though significant changes were identified, routine clinical use may be limited but may contribute to a multi marker test. Future analysis should include other biomarkers, metabolic data or clinical measurements made throughout gestation.
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Nova |
2021 |
Fisher JJ, Vanderpeet CL, Bartho LA, McKeating DR, Cuffe JSM, Holland OJ, Perkins AV, 'Mitochondrial dysfunction in placental trophoblast cells experiencing gestational diabetes mellitus', Journal of Physiology, 599 1291-1305 (2021) [C1]
Key points: Mitochondrial dysfunction is known to occur in diabetic phenotypes including type 1 and 2 diabetes mellitus. The incidence of gestational diabetes mellitus (GDM) is in... [more]
Key points: Mitochondrial dysfunction is known to occur in diabetic phenotypes including type 1 and 2 diabetes mellitus. The incidence of gestational diabetes mellitus (GDM) is increasing and defined as the onset of a diabetic phenotype during pregnancy. The role of placental mitochondria in the aetiology of GDM remains unclear and is an emerging area of research. Differing mitochondrial morphologies within the placenta may influence the pathogenesis of the disorder. This study observed mitochondrial dysfunction in GDM placenta when assessing whole tissue. Upon further investigation into mitochondrial isolates from the cytotrophoblast and syncytiotrophoblast, mitochondrial dysfunction appears exaggerated in syncytiotrophoblast. Assessing mitochondrial populations individually enabled the determination of differences between cell lineages of the placenta and established varying levels of mitochondrial dysfunction in GDM, in some instances establishing significance in pathways previously inconclusive or confounded when assessing whole tissue. This research lays the foundation for future work into mitochondrial dysfunction in the placenta and the role it may play in the aetiology of GDM. Abstract: Mitochondrial dysfunction has been associated with diabetic phenotypes, yet the involvement of placental mitochondria in gestational diabetes mellitus (GDM) remains inconclusive. This is in part complicated by the different mitochondrial subpopulations present in the two major trophoblast cell lineages of the placenta. To better elucidate the role of mitochondria in this pathology, this study examined key aspects of mitochondrial function in placentas from healthy pregnancies and those complicated by GDM in both whole tissue and isolated mitochondria. Mitochondrial content, citrate synthase activity, reactive oxygen species production and gene expression regulating metabolic, hormonal and antioxidant control was examined in placental tissue, before examining functional differences between mitochondrial isolates from cytotrophoblast (Cyto-Mito) and syncytiotrophoblast (Syncytio-Mito). Our study observed evidence of mitochondrial dysfunction across multiple pathways when assessing whole placental tissue from GDM pregnancies compared with healthy controls. Furthermore, by examining isolated mitochondria from the cytotrophoblast and syncytiotrophoblast cell lineages of the placenta we established that although both mitochondrial populations were dysfunctional, they were differentially impacted. These data highlight the need to consider changes in mitochondrial subpopulations at the feto-maternal interface when studying pregnancy pathologies.
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Nova |
2021 |
McKeating DR, Clifton VL, Hurst CP, Fisher JJ, Bennett WW, Perkins AV, 'Elemental Metabolomics for Prediction of Term Gestational Outcomes Utilising 18-Week Maternal Plasma and Urine Samples', Biological Trace Element Research, 199 26-40 (2021) [C1]
A normal pregnancy is essential to establishing a healthy start to life. Complications during have been associated with adverse perinatal outcomes and lifelong health problems. Th... [more]
A normal pregnancy is essential to establishing a healthy start to life. Complications during have been associated with adverse perinatal outcomes and lifelong health problems. The ability to identify risk factors associated with pregnancy complications early in gestation is vitally important for preventing negative foetal outcomes. Maternal nutrition has been long considered vital to a healthy pregnancy, with micronutrients and trace elements heavily implicated in maternofoetal metabolism. This study proposed the use of elemental metabolomics to study multiple elements at 18¿weeks gestation from blood plasma and urine to construct models that could predict outcomes such as small for gestational age (SGA) (n = 10), low placental weight (n = 18), and preterm birth (n = 13) from control samples (n = 87). Samples collected from the Lyell McEwin Hospital in Adelaide, South Australia, were measured for 27 plasma elements and 37 urine elements by inductively coupled plasma mass spectrometry. Exploratory analysis indicated an average selenium concentration 20¿µg/L lower than established reference ranges across all groups, low zinc in preterm (0.64¿µg/L, reference range 0.66¿1.10¿µg/L), and higher iodine in preterm and SGA gestations (preterm 102¿µg/L, SGA 111¿µg/L, reference range 40¿92¿µg/L). Using random forest algorithms with receiver operating characteristic curves, low placental weight was predicted with 86.7% accuracy using plasma, 78.6% prediction for SGA with urine, and 73.5% determination of preterm pregnancies. This study indicates that elemental metabolomic modelling could provide a means of early detection of at-risk pregnancies allowing for more targeted monitoring of mothers, with potential for early intervention strategies to be developed.
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2020 |
Bartho LA, Fisher JJ, Cuffe JSM, Perkins A, 'Mitochondrial transformations in the aging human placenta', AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM, 319 E981-E994 (2020) [C1]
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Nova |
2020 |
McKeating DR, Fisher JJ, Zhang P, Bennett WW, Perkins AV, 'Elemental metabolomics in human cord blood: Method validation and trace element quantification', JOURNAL OF TRACE ELEMENTS IN MEDICINE AND BIOLOGY, 59 (2020) [C1]
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2020 |
Fisher JJ, Bartho LA, Perkins AV, Holland OJ, 'Placental mitochondria and reactive oxygen species in the physiology and pathophysiology of pregnancy.', Clinical and experimental pharmacology & physiology, 47 176-184 (2020) [C1]
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2019 |
Hofstee P, Bartho LA, McKeating DR, Radenkovic F, McEnroe G, Fisher JJ, et al., 'Maternal selenium deficiency during pregnancy in mice increases thyroid hormone concentrations, alters placental function and reduces fetal growth', JOURNAL OF PHYSIOLOGY-LONDON, 597 5597-5617 (2019) [C1]
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2019 |
Fisher J, McKeating DR, Pennell EN, Cuffe JS, Holland OJ, Perkins AV, 'Mitochondrial isolation, cryopreservation and preliminary biochemical characterisation from placental cytotrophoblast and syncytiotrophoblast', PLACENTA, 82 1-4 (2019)
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2019 |
Fisher JJ, McKeating DR, Cuffe JS, Bianco-Miotto T, Holland OJ, Perkins A, 'Proteomic Analysis of Placental Mitochondria Following Trophoblast Differentiation', FRONTIERS IN PHYSIOLOGY, 10 (2019) [C1]
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2019 |
McKeating DR, Fisher JJ, Perkins AV, 'Elemental Metabolomics and Pregnancy Outcomes', NUTRIENTS, 11 (2019) [C1]
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2017 |
Holland O, Nitert MD, Gallo LA, Vejzovic M, Fisher JJ, Perkins AV, 'Review: Placental mitochondrial function and structure in gestational disorders', PLACENTA, 54 2-9 (2017) [C1]
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