Staff Profile
Conjoint Professor Keith Jones
Conjoint Professor
School of Biomedical Sciences and Pharmacy
EditCareer Summary
Biography
Meiosis is the name given to the two cell divisions germ cells go through following DNA replication in order to produce haploid gametes. Our research focuses on mammalian female meiosis where we use imaging based techniques (Fluorescent Protein chimeras) to explore this process in real-time. We argue that this is an important strategy when examining a dynamic process such as the meiotic cell cycle.
Meiosis is important to study because of its unique cell division; in terms of understanding a fundamental cellular developmental biology process; and also because errors in meiotic division lead to aneuploid embryos, the leading cause of early pregnancy loss in humans and the cause of Down's Syndrome.
Research Highlights: The mammalian oocyte spends most of its life arrested at prophase of the first meiotic division and we found that APCcdh1 activity was necessary in order to achieve this arrest (Reis et al., Nature Cell Biology, 2006). Recently we extended these observations to show that APCcdh1 also functioned in prometaphase to prevent aneuploidy in maturing oocytes (Reis et al., Nature Cell Biology, 2007). In the first meiotic division chromosome homologues are separated, a unique cell division, and in 2006 we found that during this division separase is needed to inhibit MPF activity (Gorr et al., Nature Cell Biology, 2006. See Nature Cell Biology, News & Views). Following exit from the first meiotic division the egg re-arrests at metaphase of the second meiotic division until sperm break this arrest with a cytosolic calcium signal. Re-arrest at metaphase of the second meiotic division requires the APC inhibitor Emi2 (Madgwick et al., Journal of Cell Biology, 2006). At fertilization the sister chromatids are separated. Another unequal cell division occurs, and the second polar body is extruded. The fertilized oocyte completes meiosis and forms a zygote.
We are very much interested in how the two meiotic divisions are controlled at a molecular level. Our approach is to over-express and visualise gene products by tagging them with a toolkit of Fluorescent Proteins (Cyan; Cerulean; Yellow, Green; Venus; and Red Fluorescent Proteins) and knock them out using antisense morpholinos.
Qualifications
- Doctor of Philosophy, University of Liverpool - UK, 11/07/1994
- Bachelor of Science (Biochemistry)(Honours), University of Leeds - UK, 07/07/1989
Research
Research keywords
- Cell Cycle
- Cell Division
- Chromosomes
- Female Fertility
- Meiosis
- Mitosis
- Oocytes
- Ovarian Biology
- Reproductive Biology
Research expertise
Mammalian reproductive biology, especially female reproduction with emphasis on oocytes and the meiotic divisions. Cell division and early embryo development, such as the transition from meiosis to mitosis, and the embryonic divisions leading up to implantation. Mitotic cell divisions of cultured cells, and the control of M-phase. Mis-segregation of chromosomes that lead to aneuploidy. Control of the meiotic divisions of oocytes with focus on the mechanisms that control faithful chromosome segregation such as the Spindle Assembly Checkpoint.
Fields of Research
| Code | Description | Percentage |
|---|
| 060699 | Physiology Not Elsewhere Classified | 45 |
| 060100 | Biochemistry And Cell Biology | 35 |
| 111499 | Paediatrics And Reproductive Medicine Not Elsewhere Classified | 20 |
EditPublications
No publications.
EditGrants and Funding
Summary
| Number of grants | 28 |
|---|
| Total funding | $5,058,322 |
|---|
For project grants received where the lead institution is other than the University of Newcastle, details are shown in italics.
- Indicates that the researcher may be seeking students for this project.
Click on a grant title below to expand the full details for that specific grant.
2012 (3 grants)
Pregnancy and Reproduction MRSP 2011-12 $369,134
Funding Body: Hunter Medical Research Institute
Project EVA: an environmentally responsible facility for interdisciplinary supercomputing applications$263,000
Funding Body: University of Newcastle
| Project Team |
|---|
| Professor Pablo Moscato, Laureate Professor Jon Borwein, Conjoint Professor Keith Jones, Conjoint Professor Chris Levi, Professor Mark Parsons, Professor Michael Ostwald, Professor Hugh Craig, Conjoint Professor Peter Greer, Associate Professor Stephan Chalup, Associate Professor Regina Berretta |
| Scheme | Role |
|---|
| Equipment Grant | Investigator |
| Total Amount | Funding Start | Funding Finish |
|---|
| $263,000 | 2012 | 2012 |
| GNo:G1100627 |
Regulation of the cell cycle by phosphorylation dependent targeting of CaMKII$20,000
Funding Body: University of Newcastle
2011 (7 grants)
Gamete-specific knockout of Fizzy-Related to examine its meiotic role in oocytes and sperm$600,000
Funding Body: ARC (Australian Research Council)
An Advanced Mass Spectrometer for Applications in Phospho-Proteomics, Glycomics and Top-Down Sequencing of Proteins$250,000
Funding Body: ARC (Australian Research Council)
SCIREQ FlexiVentFX system + FlexiVentFX extension$45,000
Funding Body: NHMRC (National Health & Medical Research Council)
| Project Team |
|---|
| Professor Phil Hansbro, Professor Paul Foster, Professor Joerg Mattes, Doctor Simon Keely, Doctor Jay Horvat, Doctor Nicole Hansbro, Doctor Ming Yang, Doctor Catherine Ptaschinski, Doctor Kelly Asquith, Doctor Gough Au, Conjoint Associate Professor Peter Wark, Laureate Professor John Aitken, Conjoint Professor Keith Jones, Professor Roger Smith, Professor Judith Black, Professor Rakesh Kumar, Professor Paul Hertzog |
| Scheme | Role |
|---|
| Equipment Grant | Investigator |
| Total Amount | Funding Start | Funding Finish |
|---|
| $45,000 | 2011 | 2011 |
| GNo:G1100037 |
Factors affecting the quality of mammalian eggs when females age.$25,000
Funding Body: University of Newcastle
| Project Team |
|---|
| Conjoint Professor Keith Jones |
| Scheme | Role |
|---|
| Near Miss Grant | Chief Investigator |
| Total Amount | Funding Start | Funding Finish |
|---|
| $25,000 | 2011 | 2011 |
| GNo:G1001042 |
An Advanced Mass Spectrometer for Applications in Phospho-Proteomics, Glycomics and Top-Down Sequencing of Proteins$15,000
Funding Body: Prince Henry's Institute of Medical Research
Eppendorf mastercycler pro with thermomixer comfort and 5430R centrifuge$15,000
Funding Body: NHMRC (National Health & Medical Research Council)
| Project Team |
|---|
| Professor Eileen McLaughlin, Professor Peter Lewis, Professor Adam McCluskey, Conjoint Professor Keith Jones, Associate Professor Brett Nixon, Doctor Shaun Roman, Doctor Jennette Sakoff, Doctor Ian Grainge, Doctor Janet Holt, Doctor Xiao Yang |
| Scheme | Role |
|---|
| Equipment Grant | Investigator |
| Total Amount | Funding Start | Funding Finish |
|---|
| $15,000 | 2011 | 2011 |
| GNo:G1100028 |
IMPLEN NanoPhotometer pearl$10,000
Funding Body: NHMRC (National Health & Medical Research Council)
| Project Team |
|---|
| Doctor Murray Cairns, Associate Professor Paul Tooney, Associate Professor Alan Brichta, Professor John Rostas, Emeritus Professor Patricia Michie, Conjoint Professor Keith Jones, Professor Ulli Schall, Associate Professor Phillip Dickson, Doctor Frederick Walker, Doctor Rick Thorne, Doctor Chris Dayas, Doctor Nikki Verrills, Doctor Janet Holt, Doctor Severine Roselli, Doctor Kathryn Skelding, Doctor Jude Weidenhofer, Associate Professor Liz Milward, Doctor Charles De Bock, Doctor Julie Merriman-Jones, Doctor Jing Qin Wu, Doctor Bing Liu, Mr Dan Johnstone, Ms Belinda Goldie, Ms Natalie Beveridge |
| Scheme | Role |
|---|
| Equipment Grant | Investigator |
| Total Amount | Funding Start | Funding Finish |
|---|
| $10,000 | 2011 | 2011 |
| GNo:G1100030 |
2010 (5 grants)
HMRI MRSP Infrastructure Grant (10-11) - PREGNANCY & REPRODUCTION$362,277
Funding Body: Hunter Medical Research Institute
Laser microdissection microscopy system for cell and development biology$350,000
Funding Body: ARC (Australian Research Council)
| Project Team |
|---|
| Professor Eileen McLaughlin, Conjoint Professor Keith Jones, Laureate Professor John Aitken, Associate Professor Brett Nixon, Doctor Shaun Roman, Associate Professor Alan Brichta, Doctor Rick Thorne, Doctor Doug Smith, Associate Professor David McCurdy, Conjoint Professor Ray Rose, Professor Christopher Grof, Conjoint Professor Leonie Ashman, Professor Gordon Burns, Doctor Brett Graham, Associate Professor Paul Tooney, Professor Roger Smith, Professor Paul Foster, Professor Trevor Day, Professor Robert Callister |
| Scheme | Role |
|---|
| Linkage Infrastructure Equipment & Facilities (LIEF) | Investigator |
| Total Amount | Funding Start | Funding Finish |
|---|
| $350,000 | 2010 | 2010 |
| GNo:G0190369 |
Laser microdissection microscopy system for cell and development biology$215,000
Funding Body: University of Newcastle
| Project Team |
|---|
| Professor Eileen McLaughlin, Conjoint Professor Keith Jones, Laureate Professor John Aitken, Associate Professor Brett Nixon, Doctor Shaun Roman, Associate Professor Alan Brichta, Doctor Rick Thorne, Doctor Doug Smith, Associate Professor David McCurdy, Conjoint Professor Ray Rose, Professor Christopher Grof, Conjoint Professor Leonie Ashman, Professor Gordon Burns, Doctor Brett Graham, Associate Professor Paul Tooney, Professor Roger Smith, Professor Paul Foster, Professor Trevor Day, Professor Robert Callister |
| Scheme | Role |
|---|
| Equipment Grant | Investigator |
| Total Amount | Funding Start | Funding Finish |
|---|
| $215,000 | 2010 | 2010 |
| GNo:G1000874 |
ABI 7500 Real Time PCR System
$34,000
Funding Body: NHMRC (National Health & Medical Research Council)
| Project Team |
|---|
| Doctor Rick Thorne, Doctor Nikki Verrills, Doctor Murray Cairns, Associate Professor Paul Tooney, Doctor Doug Smith, Professor Gordon Burns, Conjoint Professor Leonie Ashman, Conjoint Professor Keith Jones, Doctor Charles De Bock, Doctor Chris Dayas, Doctor Brett Graham, Doctor Martin Horan, Doctor Rebecca Lim, Doctor Severine Roselli, Doctor Larisa Bobrovskaya, Doctor Kathryn Skelding, Doctor Frederick Walker, Doctor Jude Weidenhofer, Associate Professor Philip Bolton, Associate Professor Alan Brichta, Professor Robert Callister, Professor Trevor Day, Associate Professor Phillip Dickson, Professor Manohar Garg, Doctor Phil Jobling, Associate Professor Derek Laver, Associate Professor Eugene Nalivaiko, Professor John Rostas |
| Scheme | Role |
|---|
| Equipment Grant | Investigator |
| Total Amount | Funding Start | Funding Finish |
|---|
| $34,000 | 2010 | 2010 |
| GNo:G1000055 |
Electron Multiplier Charge-Coupled Device Camera. Hamamatsu EM-CCD Camera C9100-13$29,079
Funding Body: NHMRC (National Health & Medical Research Council)
2009 (9 grants)
The role of the Anaphase-Promoting Complex activator Cdh1 in mammalian oocytes and aneuploidy$506,250
Funding Body: NHMRC (National Health & Medical Research Council)
An Advanced Mass Spectrometry Facility for Applications in Proteomics and Organic Chemistry$495,000
Funding Body: ARC (Australian Research Council)
| Project Team |
|---|
| Laureate Professor John Aitken, Professor Adam McCluskey, Doctor Mark Baker, Doctor Nikki Verrills, Professor Marcel Maeder, Doctor Xiaojing Zhou, Professor Eileen McLaughlin, Associate Professor Brett Nixon, Doctor Shaun Roman, Conjoint Professor Ray Rose, Professor Hugh Dunstan, Professor Christopher Grof, Professor Roger Smith, Conjoint Professor Peter Gibson, Conjoint Professor Alison Jones, Professor Mike Calford, Conjoint Professor Keith Jones, Doctor Rick Thorne, Emeritus Professor Peter Dunkley, Professor Paul Foster, Conjoint Professor Leonie Ashman, Professor Gordon Burns, Associate Professor Phillip Dickson, Professor John Rostas, Professor Rodney Scott, Associate Professor Paul Tooney, Professor Phil Hansbro, Professor Pablo Moscato, Professor Paul Dastoor, Professor Peter Lewis |
| Scheme | Role |
|---|
| Linkage Infrastructure Equipment & Facilities (LIEF) | Investigator |
| Total Amount | Funding Start | Funding Finish |
|---|
| $495,000 | 2009 | 2009 |
| GNo:G0189122 |
Elucidation of the signalling pathways during fertilization in mammals$325,000
Funding Body: ARC (Australian Research Council)
| Project Team |
|---|
| Conjoint Professor Keith Jones, Dr Olaf Stemmann |
| Scheme | Role |
|---|
| Discovery Projects | Chief Investigator |
| Total Amount | Funding Start | Funding Finish |
|---|
| $325,000 | 2009 | 2011 |
| GNo:G0188824 |
HMRI MRSP Infrastructure Grant (09-10) Pregnancy and Reproduction$296,789
Funding Body: Hunter Medical Research Institute
Confocal Laser Scanning Microscopy for Live Cell Imaging$275,000
Funding Body: ARC (Australian Research Council)
| Project Team |
|---|
| Conjoint Professor Keith Jones, Professor Eileen McLaughlin, Laureate Professor John Aitken, Conjoint Professor Ray Rose, Emeritus Professor John Patrick, Conjoint Professor Christina Offler, Associate Professor David McCurdy, Conjoint Professor Leonie Ashman, Professor Gordon Burns, Professor Dirk Van Helden, Doctor Nikki Verrills, Associate Professor Brett Nixon, Doctor Shaun Roman, Associate Professor Yong-Ling Ruan, Doctor Rick Thorne, Professor Mike Calford |
| Scheme | Role |
|---|
| Linkage Infrastructure Equipment & Facilities (LIEF) | Chief Investigator |
| Total Amount | Funding Start | Funding Finish |
|---|
| $275,000 | 2009 | 2009 |
| GNo:G0189038 |
Aging eggs: understanding the molecular mechanisms of declining female fertility$79,489
Funding Body: Hunter Medical Research Institute
An Advanced Mass Spectrometry Facility for Applications in Proteomics and Organic Chemistry$50,000
Funding Body: ARC (Australian Research Council)
| Project Team |
|---|
| Laureate Professor John Aitken, Professor Adam McCluskey, Doctor Mark Baker, Doctor Nikki Verrills, Professor Marcel Maeder, Doctor Xiaojing Zhou, Professor Eileen McLaughlin, Associate Professor Brett Nixon, Doctor Shaun Roman, Conjoint Professor Ray Rose, Professor Hugh Dunstan, Professor Christopher Grof, Professor Roger Smith, Conjoint Professor Peter Gibson, Conjoint Professor Alison Jones, Professor Mike Calford, Conjoint Professor Keith Jones, Doctor Rick Thorne, Emeritus Professor Peter Dunkley, Professor Paul Foster, Conjoint Professor Leonie Ashman, Professor Gordon Burns, Associate Professor Phillip Dickson, Professor John Rostas, Professor Rodney Scott, Associate Professor Paul Tooney, Professor Phil Hansbro, Professor Pablo Moscato, Professor Paul Dastoor, Professor Peter Lewis |
| Scheme | Role |
|---|
| Linkage Infrastructure Equipment & Facilities (LIEF) Partner funding | Investigator |
| Total Amount | Funding Start | Funding Finish |
|---|
| $50,000 | 2009 | 2009 |
| GNo:G0189948 |
Leica VT1200S - Fully automated vibrating blade microtome$16,209
Funding Body: NHMRC (National Health & Medical Research Council)
| Project Team |
|---|
| Professor Robert Callister, Associate Professor Alan Brichta, Conjoint Professor Keith Jones, Associate Professor Jon Hirst, Doctor Brett Graham, Associate Professor Philip Bolton, Doctor Phil Jobling, Associate Professor Paul Tooney, Doctor Angela McPherson, Doctor Rebecca Lim, Doctor Ramatis De Oliveira, Mr Matthew Walsh |
| Scheme | Role |
|---|
| Equipment Grant | Investigator |
| Total Amount | Funding Start | Funding Finish |
|---|
| $16,209 | 2009 | 2009 |
| GNo:G0189842 |
GRC Fertilization and Egg Activation, Plymouth USA, 12-17 July 2009$1,700
Funding Body: University of Newcastle - Faculty of Health
2008 (4 grants)
The function of gametogenenin in male fertility and embryo genesis$230,500
Funding Body: NHMRC (National Health & Medical Research Council)
| Project Team |
|---|
| Conjoint Professor Keith Jones, Dr M O'Bryan |
| Scheme | Role |
|---|
| Project Grant | Chief Investigator |
| Total Amount | Funding Start | Funding Finish |
|---|
| $230,500 | 2008 | 2010 |
| GNo:G0188417 |
Olympus Fluoview FV1000-IX81 Microscope$141,970
Funding Body: NHMRC (National Health & Medical Research Council)
| Project Team |
|---|
| Conjoint Professor Keith Jones |
| Scheme | Role |
|---|
| Equipment Grant | Chief Investigator |
| Total Amount | Funding Start | Funding Finish |
|---|
| $141,970 | 2008 | 2008 |
| GNo:G0188673 |
Colibri high-performance LED illumination system for fluorescence live cell microscopy$23,225
Funding Body: NHMRC (National Health & Medical Research Council)
Programmed mammalian oocyte activation: the production line model of primordial follicle development$14,700
Funding Body: University of Newcastle
EditResearch Supervision
| Number of current supervisions | 2 |
|---|
| Total current UoN PhD EFTSL | 0.45 |
|---|
For supervisions undertaken at an institution other that the University of Newcastle, details are shown in italics, and the institution name is listed below the program name.
Current Supervision
| Commenced | Proposed Completion | Program | Supervisor Type | Research Title |
|---|
| 2011 | 2016 | PhD (Biological Sciences) | Co-Supervisor | Gamete-Specific Knockout of Fizzy-Related 1 to Examine its Meiotic Role in Spermatozoa |
| 2010 | 2014 | PhD (Human Physiology) | Co-Supervisor | Elucidation of Higher Meiotic Errors on Oocyte from Advanced Maternat Ages through Kinetochore Tracking |
Past Supervision
| Year | Program | Supervisor Type | Research Title |
|---|
| 2013 | PhD (Human Physiology) | Principal Supervisor | Role of Fzr1 in Embryogenesis |
| 2013 | PhD (Human Physiology) | Principal Supervisor | DNA Repair and the Fanconi Anemia Pathway: Insights into Female Meiosis and Mitosis |
| 2012 | M Philosophy (Human Physiolog) | Principal Supervisor | Characterisation of an Oocyte Specific Knockout Model of Cdh1 |
| 2012 | PhD (Human Physiology) | Principal Supervisor | Control of Chromosome Segregation in Mouse Oocytes |