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Biography

Meiosis is the name given to the two cell divisions germ cells go through following DNA replication in order to produce haploid gametes. Our research focuses on mammalian female meiosis where we use imaging based techniques (Fluorescent Protein chimeras) to explore this process in real-time. We argue that this is an important strategy when examining a dynamic process such as the meiotic cell cycle.

Meiosis is important to study because of its unique cell division; in terms of understanding a fundamental cellular developmental biology process; and also because errors in meiotic division lead to aneuploid embryos, the leading cause of early pregnancy loss in humans and the cause of Down's Syndrome.

Research Highlights: The mammalian oocyte spends most of its life arrested at prophase of the first meiotic division and we found that APCcdh1 activity was necessary in order to achieve this arrest (Reis et al., Nature Cell Biology, 2006). Recently we extended these observations to show that APCcdh1 also functioned in prometaphase to prevent aneuploidy in maturing oocytes (Reis et al., Nature Cell Biology, 2007). In the first meiotic division chromosome homologues are separated, a unique cell division, and in 2006 we found that during this division separase is needed to inhibit MPF activity (Gorr et al., Nature Cell Biology, 2006. See Nature Cell Biology, News & Views). Following exit from the first meiotic division the egg re-arrests at metaphase of the second meiotic division until sperm break this arrest with a cytosolic calcium signal. Re-arrest at metaphase of the second meiotic division requires the APC inhibitor Emi2 (Madgwick et al., Journal of Cell Biology, 2006). At fertilization the sister chromatids are separated. Another unequal cell division occurs, and the second polar body is extruded. The fertilized oocyte completes meiosis and forms a zygote.

We are very much interested in how the two meiotic divisions are controlled at a molecular level. Our approach is to over-express and visualise gene products by tagging them with a toolkit of Fluorescent Proteins (Cyan; Cerulean; Yellow, Green; Venus; and Red Fluorescent Proteins) and knock them out using antisense morpholinos.

Qualifications

  • Doctor of Philosophy, University of Liverpool - UK, 11/07/1994
  • Bachelor of Science (Biochemistry)(Honours), University of Leeds - UK, 07/07/1989

Research

Research keywords

  • Cell Cycle
  • Cell Division
  • Chromosomes
  • Female Fertility
  • Meiosis
  • Mitosis
  • Oocytes
  • Ovarian Biology
  • Reproductive Biology

Research expertise

Mammalian reproductive biology, especially female reproduction with emphasis on oocytes and the meiotic divisions. Cell division and early embryo development, such as the transition from meiosis to mitosis, and the embryonic divisions leading up to implantation. Mitotic cell divisions of cultured cells, and the control of M-phase. Mis-segregation of chromosomes that lead to aneuploidy. Control of the meiotic divisions of oocytes with focus on the mechanisms that control faithful chromosome segregation such as the Spindle Assembly Checkpoint.

Fields of Research

CodeDescriptionPercentage
060699Physiology Not Elsewhere Classified45
060100Biochemistry And Cell Biology35
111499Paediatrics And Reproductive Medicine Not Elsewhere Classified20
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Publications

No publications.

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Grants and Funding

Summary

Number of grants28
Total funding$5,058,322

For project grants received where the lead institution is other than the University of Newcastle, details are shown in italics.

- Indicates that the researcher may be seeking students for this project.

Click on a grant title below to expand the full details for that specific grant.

2012 (3 grants)

Pregnancy and Reproduction MRSP 2011-12 $369,134
Funding Body: Hunter Medical Research Institute

Project Team
Professor Roger Smith, Laureate Professor John Aitken, Conjoint Professor Keith Jones
SchemeRole
MRSP Infrastructure GrantInvestigator
Total AmountFunding StartFunding Finish
$369,13420122012
GNo:G1101192

Project EVA: an environmentally responsible facility for interdisciplinary supercomputing applications$263,000
Funding Body: University of Newcastle

Regulation of the cell cycle by phosphorylation dependent targeting of CaMKII$20,000
Funding Body: University of Newcastle

Project Team
Doctor Nikki Verrills, Doctor Kathryn Skelding, Professor John Rostas, Associate Professor Phillip Dickson, Conjoint Professor Keith Jones
SchemeRole
Near Miss GrantInvestigator
Total AmountFunding StartFunding Finish
$20,00020122012
GNo:G1200679

2011 (7 grants)

Gamete-specific knockout of Fizzy-Related to examine its meiotic role in oocytes and sperm$600,000
Funding Body: ARC (Australian Research Council)

Project Team
Professor Eileen McLaughlin, Conjoint Professor Keith Jones, Professor Sergio Moreno
SchemeRole
Discovery ProjectsInvestigator
Total AmountFunding StartFunding Finish
$600,00020112014
GNo:G1000020

An Advanced Mass Spectrometer for Applications in Phospho-Proteomics, Glycomics and Top-Down Sequencing of Proteins$250,000
Funding Body: ARC (Australian Research Council)

Project Team
Doctor Mark Baker, Laureate Professor John Aitken, Professor Roger Smith, Conjoint Professor Keith Jones, Conjoint Professor Ray Rose, Professor Lois Salamonsen
SchemeRole
Linkage Infrastructure Equipment & Facilities (LIEF)Investigator
Total AmountFunding StartFunding Finish
$250,00020112011
GNo:G1000632

SCIREQ FlexiVentFX system + FlexiVentFX extension$45,000
Funding Body: NHMRC (National Health & Medical Research Council)

Project Team
Professor Phil Hansbro, Professor Paul Foster, Professor Joerg Mattes, Doctor Simon Keely, Doctor Jay Horvat, Doctor Nicole Hansbro, Doctor Ming Yang, Doctor Catherine Ptaschinski, Doctor Kelly Asquith, Doctor Gough Au, Conjoint Associate Professor Peter Wark, Laureate Professor John Aitken, Conjoint Professor Keith Jones, Professor Roger Smith, Professor Judith Black, Professor Rakesh Kumar, Professor Paul Hertzog
SchemeRole
Equipment GrantInvestigator
Total AmountFunding StartFunding Finish
$45,00020112011
GNo:G1100037

Factors affecting the quality of mammalian eggs when females age.$25,000
Funding Body: University of Newcastle

Project Team
Conjoint Professor Keith Jones
SchemeRole
Near Miss GrantChief Investigator
Total AmountFunding StartFunding Finish
$25,00020112011
GNo:G1001042

An Advanced Mass Spectrometer for Applications in Phospho-Proteomics, Glycomics and Top-Down Sequencing of Proteins$15,000
Funding Body: Prince Henry's Institute of Medical Research

Project Team
Doctor Mark Baker, Laureate Professor John Aitken, Professor Roger Smith, Conjoint Professor Keith Jones, Conjoint Professor Ray Rose, Professor Lois Salamonsen
SchemeRole
Linkage Infrastructure Equipment & Facilities (LIEF) Partner fundingInvestigator
Total AmountFunding StartFunding Finish
$15,00020112011
GNo:G1100844

Eppendorf mastercycler pro with thermomixer comfort and 5430R centrifuge$15,000
Funding Body: NHMRC (National Health & Medical Research Council)

Project Team
Professor Eileen McLaughlin, Professor Peter Lewis, Professor Adam McCluskey, Conjoint Professor Keith Jones, Associate Professor Brett Nixon, Doctor Shaun Roman, Doctor Jennette Sakoff, Doctor Ian Grainge, Doctor Janet Holt, Doctor Xiao Yang
SchemeRole
Equipment GrantInvestigator
Total AmountFunding StartFunding Finish
$15,00020112011
GNo:G1100028

IMPLEN NanoPhotometer pearl$10,000
Funding Body: NHMRC (National Health & Medical Research Council)

Project Team
Doctor Murray Cairns, Associate Professor Paul Tooney, Associate Professor Alan Brichta, Professor John Rostas, Emeritus Professor Patricia Michie, Conjoint Professor Keith Jones, Professor Ulli Schall, Associate Professor Phillip Dickson, Doctor Frederick Walker, Doctor Rick Thorne, Doctor Chris Dayas, Doctor Nikki Verrills, Doctor Janet Holt, Doctor Severine Roselli, Doctor Kathryn Skelding, Doctor Jude Weidenhofer, Associate Professor Liz Milward, Doctor Charles De Bock, Doctor Julie Merriman-Jones, Doctor Jing Qin Wu, Doctor Bing Liu, Mr Dan Johnstone, Ms Belinda Goldie, Ms Natalie Beveridge
SchemeRole
Equipment GrantInvestigator
Total AmountFunding StartFunding Finish
$10,00020112011
GNo:G1100030

2010 (5 grants)

HMRI MRSP Infrastructure Grant (10-11) - PREGNANCY & REPRODUCTION$362,277
Funding Body: Hunter Medical Research Institute

Project Team
Professor Roger Smith, Laureate Professor John Aitken, Conjoint Professor Keith Jones
SchemeRole
MRSP Infrastructure GrantInvestigator
Total AmountFunding StartFunding Finish
$362,27720102011
GNo:G1100474

Laser microdissection microscopy system for cell and development biology$350,000
Funding Body: ARC (Australian Research Council)

Laser microdissection microscopy system for cell and development biology$215,000
Funding Body: University of Newcastle

ABI 7500 Real Time PCR System $34,000
Funding Body: NHMRC (National Health & Medical Research Council)

Electron Multiplier Charge-Coupled Device Camera. Hamamatsu EM-CCD Camera C9100-13$29,079
Funding Body: NHMRC (National Health & Medical Research Council)

Project Team
Conjoint Professor Keith Jones, Professor Eileen McLaughlin, Professor Dirk Van Helden, Doctor Rick Thorne
SchemeRole
Equipment GrantChief Investigator
Total AmountFunding StartFunding Finish
$29,07920102010
GNo:G1000052

2009 (9 grants)

The role of the Anaphase-Promoting Complex activator Cdh1 in mammalian oocytes and aneuploidy$506,250
Funding Body: NHMRC (National Health & Medical Research Council)

Project Team
Conjoint Professor Keith Jones, Professor Eileen McLaughlin
SchemeRole
Project GrantChief Investigator
Total AmountFunding StartFunding Finish
$506,25020092011
GNo:G0188850

An Advanced Mass Spectrometry Facility for Applications in Proteomics and Organic Chemistry$495,000
Funding Body: ARC (Australian Research Council)

Elucidation of the signalling pathways during fertilization in mammals$325,000
Funding Body: ARC (Australian Research Council)

Project Team
Conjoint Professor Keith Jones, Dr Olaf Stemmann
SchemeRole
Discovery ProjectsChief Investigator
Total AmountFunding StartFunding Finish
$325,00020092011
GNo:G0188824

HMRI MRSP Infrastructure Grant (09-10) Pregnancy and Reproduction$296,789
Funding Body: Hunter Medical Research Institute

Project Team
Professor Roger Smith, Laureate Professor John Aitken, Conjoint Professor Keith Jones
SchemeRole
MRSP Infrastructure GrantInvestigator
Total AmountFunding StartFunding Finish
$296,78920092010
GNo:G1000642

Confocal Laser Scanning Microscopy for Live Cell Imaging$275,000
Funding Body: ARC (Australian Research Council)

Aging eggs: understanding the molecular mechanisms of declining female fertility$79,489
Funding Body: Hunter Medical Research Institute

Project Team
Conjoint Professor Keith Jones, Professor Eileen McLaughlin
SchemeRole
Project GrantChief Investigator
Total AmountFunding StartFunding Finish
$79,48920092010
GNo:G0189795

An Advanced Mass Spectrometry Facility for Applications in Proteomics and Organic Chemistry$50,000
Funding Body: ARC (Australian Research Council)

Leica VT1200S - Fully automated vibrating blade microtome$16,209
Funding Body: NHMRC (National Health & Medical Research Council)

Project Team
Professor Robert Callister, Associate Professor Alan Brichta, Conjoint Professor Keith Jones, Associate Professor Jon Hirst, Doctor Brett Graham, Associate Professor Philip Bolton, Doctor Phil Jobling, Associate Professor Paul Tooney, Doctor Angela McPherson, Doctor Rebecca Lim, Doctor Ramatis De Oliveira, Mr Matthew Walsh
SchemeRole
Equipment GrantInvestigator
Total AmountFunding StartFunding Finish
$16,20920092009
GNo:G0189842

GRC Fertilization and Egg Activation, Plymouth USA, 12-17 July 2009$1,700
Funding Body: University of Newcastle - Faculty of Health

Project Team
Conjoint Professor Keith Jones
SchemeRole
Travel GrantChief Investigator
Total AmountFunding StartFunding Finish
$1,70020092009
GNo:G0190133

2008 (4 grants)

The function of gametogenenin in male fertility and embryo genesis$230,500
Funding Body: NHMRC (National Health & Medical Research Council)

Project Team
Conjoint Professor Keith Jones, Dr M O'Bryan
SchemeRole
Project GrantChief Investigator
Total AmountFunding StartFunding Finish
$230,50020082010
GNo:G0188417

Olympus Fluoview FV1000-IX81 Microscope$141,970
Funding Body: NHMRC (National Health & Medical Research Council)

Project Team
Conjoint Professor Keith Jones
SchemeRole
Equipment GrantChief Investigator
Total AmountFunding StartFunding Finish
$141,97020082008
GNo:G0188673

Colibri high-performance LED illumination system for fluorescence live cell microscopy$23,225
Funding Body: NHMRC (National Health & Medical Research Council)

Project Team
Doctor Rick Thorne, Dr Charles De Bock, Professor Xu Dong Zhang, Doctor Lisa Lincz, Professor Gordon Burns, Conjoint Professor Peter Hersey, Professor Dirk Van Helden, Conjoint Professor Keith Jones, Professor Roger Smith
SchemeRole
Equipment GrantInvestigator
Total AmountFunding StartFunding Finish
$23,22520082008
GNo:G0188545

Programmed mammalian oocyte activation: the production line model of primordial follicle development$14,700
Funding Body: University of Newcastle

Project Team
Professor Eileen McLaughlin, Conjoint Professor Keith Jones
SchemeRole
Pilot GrantInvestigator
Total AmountFunding StartFunding Finish
$14,70020082008
GNo:G0189084
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Research Supervision

Number of current supervisions2
Total current UoN PhD EFTSL0.45

For supervisions undertaken at an institution other that the University of Newcastle, details are shown in italics, and the institution name is listed below the program name.

Current Supervision

CommencedProposed
Completion
ProgramSupervisor TypeResearch Title
20112016PhD (Biological Sciences)Co-SupervisorGamete-Specific Knockout of Fizzy-Related 1 to Examine its Meiotic Role in Spermatozoa
20102014PhD (Human Physiology)Co-SupervisorElucidation of Higher Meiotic Errors on Oocyte from Advanced Maternat Ages through Kinetochore Tracking

Past Supervision

YearProgramSupervisor TypeResearch Title
2013PhD (Human Physiology)Principal SupervisorRole of Fzr1 in Embryogenesis
2013PhD (Human Physiology)Principal SupervisorDNA Repair and the Fanconi Anemia Pathway: Insights into Female Meiosis and Mitosis
2012M Philosophy (Human Physiolog)Principal SupervisorCharacterisation of an Oocyte Specific Knockout Model of Cdh1
2012PhD (Human Physiology)Principal SupervisorControl of Chromosome Segregation in Mouse Oocytes
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Conjoint Professor Keith Jones

Work Phone(02) 4921 6682
Fax(02) 4921 7903
Email
PositionConjoint Professor
School of Biomedical Sciences and Pharmacy
Faculty of Health and Medicine
The University of Newcastle, Australia
Focus AreaHuman Physiology
Office
MS612,
Medical Sciences,
Callaghan
University Drive
Callaghan NSW 2308
Australia
URL:www.newcastle.edu.au/profile/keith-jones