Dr Melissa Tadros

Dr Melissa Tadros

Lecturer

School of Biomedical Sciences and Pharmacy

Career Summary

Biography

The ability to sense a cool summer breeze, an insect crawling on the skin, a full stomach or food that is too hot to eat depends upon a carefully organized sensory pathway from the body to the brain. When this pathway is disrupted, the consequences can be devastating. 

 

Dr Melissa Tadros uses sophisticated neurological techniques to examine this sensory pathway in high resolution, with a main focus on how early life events, including infection, can change the developmental plan for the nerves along this passage. 

 

In parallel with her research, Melissa has a passion for community engagement and the dissemination of knowledge across platforms. She has a wealth of teaching experience, and is known for delivering quality anatomy courses to first year students of allied health programs. Melissa has also been an integral organiser for a number of local and national conferences. 

 

Melissa has a PhD in Neuroscience (University of Newcastle, 2011) and postgraduate qualifications in tertiary education. She brings this wide variety of experiences together to provide mentoring and guidance for younger members of her research team.

Qualifications

  • PhD, University of Newcastle
  • Bachelor of Biomedical Sciences, University of Newcastle
  • Bachelor of Biomedical Sciences (Hons), University of Newcastle

Keywords

  • Brainstem
  • Developmental neurobiology
  • Early-life events
  • Intrinsic excitability
  • Motoneurons
  • Motor control
  • Neurophysiology
  • Neuroscience
  • Pain
  • Sensory neurobiology
  • Spinal cord

Fields of Research

Code Description Percentage
110603 Motor Control 20
110999 Neurosciences not elsewhere classified 50
111699 Medical Physiology not elsewhere classified 30

Professional Experience

UON Appointment

Title Organisation / Department
Research Associate University of Newcastle
School of Biomedical Sciences and Pharmacy
Australia
Lecturer University of Newcastle
School of Biomedical Sciences and Pharmacy
Australia
Casual Lecturer University of Newcastle
School of Biomedical Sciences and Pharmacy
Australia
Casual Lecturer University of Newcastle
School of Biomedical Sciences and Pharmacy
Australia
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Publications

For publications that are currently unpublished or in-press, details are shown in italics.


Journal article (15 outputs)

Year Citation Altmetrics Link
2018 Tadros MA, Graham BA, Callister RJ, 'Moving functional classification of dorsal horn neurons from art to science', JOURNAL OF PHYSIOLOGY-LONDON, 596 1543-1544 (2018)
DOI 10.1113/JP275870
Citations Scopus - 1Web of Science - 1
Co-authors Brett Graham, Robert Callister
2018 Tadros MA, Zouikr I, Hodgson DM, Callister RJ, 'Excitability of rat superficial dorsal horn neurons following a neonatal immune challenge', Frontiers in Neurology, 9 (2018) [C1]
DOI 10.3389/fneur.2018.00743
Co-authors Robert Callister, Deborah Hodgson
2016 Tadros MA, Fuglevand AJ, Brichta AM, Callister RJ, 'Intrinsic excitability differs between murine hypoglossal and spinal motoneurons.', Journal of neurophysiology, 115 2672-2680 (2016) [C1]
DOI 10.1152/jn.01114.2015
Citations Scopus - 4Web of Science - 4
Co-authors Robert Callister, Alan Brichta
2015 Tadros MA, Lim R, Hughes DI, Brichta AM, Callister RJ, 'Electrical maturation of spinal neurons in the human fetus: Comparison of ventral and dorsal horn', Journal of Neurophysiology, 114 2661-2671 (2015) [C1]

© 2015 the American Physiological Society. The spinal cord is critical for modifying and relaying sensory information to, and motor commands from, higher centers in the central ne... [more]

© 2015 the American Physiological Society. The spinal cord is critical for modifying and relaying sensory information to, and motor commands from, higher centers in the central nervous system to initiate and maintain contextually relevant locomotor responses. Our understanding of how spinal sensorimotor circuits are established during in utero development is based largely on studies in rodents. In contrast, there is little functional data on the development of sensory and motor systems in humans. Here, we use patch-clamp electrophysiology to examine the development of neuronal excitability in human fetal spinal cords (10¿18 wk gestation; WG). Transverse spinal cord slices (300 µm thick) were prepared, and recordings were made, from visualized neurons in either the ventral (VH) or dorsal horn (DH) at 32°C. Action potentials (APs) could be elicited in VH neurons throughout the period examined, but only after 16 WG in DH neurons. At this age, VH neurons discharged multiple APs, whereas most DH neurons discharged single APs. In addition, at 16¿18 WG, VH neurons also displayed larger AP and after-hyperpolarization amplitudes than DH neurons. Between 10 and 18 WG, the intrinsic properties of VH neurons changed markedly, with input resistance decreasing and AP and after-hyperpolarization amplitudes increasing. These findings are consistent with the hypothesis that VH motor circuitry matures more rapidly than the DH circuits that are involved in processing tactile and nociceptive information.

DOI 10.1152/jn.00682.2015
Citations Scopus - 3Web of Science - 3
Co-authors Alan Brichta, Rebecca Lim, Robert Callister
2015 Tadros MA, Farrell KE, Graham BA, Brichta AM, Callister RJ, 'Properties of sodium currents in neonatal and young adult mouse superficial dorsal horn neurons', Molecular Pain, 11 (2015) [C1]

© Tadros et al.; licensee BioMed Central. Background: Superficial dorsal horn (SDH) neurons process nociceptive information and their excitability is partly determined by the prop... [more]

© Tadros et al.; licensee BioMed Central. Background: Superficial dorsal horn (SDH) neurons process nociceptive information and their excitability is partly determined by the properties of voltage-gated sodium channels. Recently, we showed the excitability and action potential properties of mouse SDH neurons change markedly during early postnatal development. Here we compare sodium currents generated in neonate (P0-5) and young adult (=P21) SDH neurons. Results: Whole cell recordings were obtained from lumbar SDH neurons in transverse spinal cord slices (CsF internal, 32°C). Fast activating and inactivating TTX-sensitive inward currents were evoked by depolarization from a holding potential of 100mV. Poorly clamped currents, based on a deflection in the IV relationship at potentials between 60 and 50mV, were not accepted for analysis. Current density and decay time increased significantly between the first and third weeks of postnatal development, whereas time to peak was similar at both ages. This was accompanied by more subtle changes in activation range and steady state inactivation. Recovery from inactivation was slower and TTX-sensitivity was reduced in young adult neurons. Conclusions: Our study suggests sodium channel expression changes markedly during early postnatal development in mouse SDH neurons. The methods employed in this study can now be applied to future investigations of spinal cord sodium channel plasticity in murine pain models.

DOI 10.1186/s12990-015-0014-5
Citations Scopus - 1Web of Science - 1
Co-authors Alan Brichta, Robert Callister
2015 Tadros MA, Farrell KE, Graham BA, Brichta AM, Callister RJ, 'Properties of sodium currents in neonatal and young adult mouse superficial dorsal horn neurons.', Molecular pain, 11 17 (2015)
DOI 10.1186/s12990-015-0014-5
Co-authors Brett Graham, Robert Callister, Alan Brichta
2014 Tadros MA, Farrell KE, Schofield PR, Brichta AM, Graham BA, Fuglevand AJ, Callister RJ, 'Intrinsic and synaptic homeostatic plasticity in motoneurons from mice with glycine receptor mutations', Journal of Neurophysiology, 111 1487-1498 (2014) [C1]

Inhibitory synaptic inputs to hypoglossal motoneurons (HMs) are important for modulating excitability in brainstem circuits. Here we ask whether reduced inhibition, as occurs in t... [more]

Inhibitory synaptic inputs to hypoglossal motoneurons (HMs) are important for modulating excitability in brainstem circuits. Here we ask whether reduced inhibition, as occurs in three murine mutants with distinct naturally occurring mutations in the glycine receptor (GlyR), leads to intrinsic and/or synaptic homeostatic plasticity. Whole cell recordings were obtained from HMs in transverse brainstem slices from wild-type (wt), spasmodic (spd), spastic (spa), and oscillator (ot) mice (C57Bl/6, approximately postnatal day 21). Passive and action potential (AP) properties in spd and ot HMs were similar to wt. In contrast, spa HMs had lower input resistances, more depolarized resting membrane potentials, higher rheobase currents, smaller AP amplitudes, and slower afterhyperpolarization current decay times. The excitability of HMs, assessed by "gain" in injected current/firing-frequency plots, was similar in all strains whereas the incidence of rebound spiking was increased in spd. The difference between recruitment and derecruitment current (i.e., ¿I) for AP discharge during ramp current injection was more negative in spa and ot. GABAAminiature inhibitory postsynaptic current (mIPSC) amplitude was increased in spa and ot but not spd, suggesting diminished glycinergic drive leads to compensatory adjustments in the other major fast inhibitory synaptic transmitter system in these mutants. Overall, our data suggest long-term reduction in glycinergic drive to HMs results in changes in intrinsic and synaptic properties that are consistent with homeostatic plasticity in spa and ot but not in spd. We propose such plasticity is an attempt to stabilize HM output, which succeeds in spa but fails in ot. © 2014 the American Physiological Society.

DOI 10.1152/jn.00728.2013
Citations Scopus - 7Web of Science - 7
Co-authors Brett Graham, Alan Brichta, Robert Callister
2014 Zouikr I, Tadros MA, Barouei J, Beagley KW, Clifton VL, Callister RJ, Hodgson DM, 'Altered nociceptive, endocrine, and dorsal horn neuron responses in rats following a neonatal immune challenge', PSYCHONEUROENDOCRINOLOGY, 41 1-12 (2014) [C1]
DOI 10.1016/j.psyneuen.2013.11.016
Citations Scopus - 16Web of Science - 15
Co-authors Deborah Hodgson, Robert Callister
2014 Lim R, Drury HR, Tadros MA, Callister RJ, Brichta AM, Camp AJ, 'Preliminary Characterization of Voltage-Activated Whole-Cell Currents in Developing Human Vestibular Hair Cells and Calyx Afferent Terminals', Journal of the Association for Research in Otolaryngology, (2014) [C1]

We present preliminary functional data from human vestibular hair cells and primary afferent calyx terminals during fetal development. Whole-cell recordings were obtained from hai... [more]

We present preliminary functional data from human vestibular hair cells and primary afferent calyx terminals during fetal development. Whole-cell recordings were obtained from hair cells or calyx terminals in semi-intact cristae prepared from human fetuses aged between 11 and 18 weeks gestation (WG). During early fetal development (11-14 WG), hair cells expressed whole-cell conductances that were qualitatively similar but quantitatively smaller than those observed previously in mature rodent type II hair cells. As development progressed (15-18 WG), peak outward conductances increased in putative type II hair cells but did not reach amplitudes observed in adult human hair cells. Type I hair cells express a specific low-voltage activating conductance, G. A similar current was first observed at 15 WG but remained relatively small, even at 18 WG. The presence of a "collapsing" tail current indicates a maturing type I hair cell phenotype and suggests the presence of a surrounding calyx afferent terminal. We were also able to record from calyx afferent terminals in 15-18 WG cristae. In voltage clamp, these terminals exhibited fast inactivating inward as well as slower outward conductances, and in current clamp, discharged a single action potential during depolarizing steps. Together, these data suggest the major functional characteristics of type I and type II hair cells and calyx terminals are present by 18 WG. Our study also describes a new preparation for the functional investigation of key events that occur during maturation of human vestibular organs. © 2014 The Author(s).

DOI 10.1007/s10162-014-0471-y
Citations Scopus - 7Web of Science - 7
Co-authors Rebecca Lim, Alan Brichta, Robert Callister
2014 Harris BM, Hughes DI, Bolton PS, Tadros MA, Callister RJ, Graham BA, 'Contrasting alterations to synaptic and intrinsic properties in upper-cervical superficial dorsal horn neurons following acute neck muscle inflammation', MOLECULAR PAIN, 10 (2014) [C1]
DOI 10.1186/1744-8069-10-25
Citations Scopus - 4Web of Science - 4
Co-authors Philip Bolton, Robert Callister, Brett Graham
2013 Zouikr I, Tadros MA, Clifton VL, Beagley KW, Hodgson DM, 'Low Formalin Concentrations Induce Fine-Tuned Responses That Are Sex and Age-Dependent: A Developmental Study', PLOS ONE, 8 (2013) [C1]
DOI 10.1371/journal.pone.0053384
Citations Scopus - 7Web of Science - 8
Co-authors Deborah Hodgson
2012 Tadros MA, Harris B, Anderson WB, Brichta AM, Graham BA, Callister RJ, 'Are all spinal segments equal: Intrinsic membrane properties of superficial dorsal horn neurons in the developing and mature mouse spinal cord', Journal of Physiology, 590 2409-2425 (2012) [C1]
Citations Scopus - 11Web of Science - 12
Co-authors Robert Callister, Brett Graham, Alan Brichta
2011 Graham BA, Tadros MA, Schofield PR, Callister RJ, 'Probing glycine receptor stoichiometry in superficial dorsal horn neurones using the spasmodic mouse', Journal of Physiology, 589 2459-2474 (2011) [C1]
DOI 10.1113/jphysiol.2011.206326
Citations Scopus - 23Web of Science - 23
Co-authors Robert Callister, Brett Graham
2011 Pringle KG, Tadros MA, Callister RJ, Lumbers ER, 'The expression and localization of the human placental prorenin/renin-angiotensin system throughout pregnancy: Roles in trophoblast invasion and angiogenesis?', Placenta, 32 956-962 (2011) [C1]
Citations Scopus - 48Web of Science - 47
Co-authors E Lumbers, Robert Callister, Kirsty Pringle
2009 Tadros MA, Graham BA, Brichta AM, Callister RJ, 'Evidence for a critical period in the development of excitability and potassium currents in mouse lumbar superficial dorsal horn neurons', Journal of Neurophysiology, 101 1800-1812 (2009) [C1]
DOI 10.1152/jn.90755.2008
Citations Scopus - 33Web of Science - 31
Co-authors Brett Graham, Alan Brichta, Robert Callister
Show 12 more journal articles

Conference (12 outputs)

Year Citation Altmetrics Link
2015 Tadros M, Lim R, Hughes D, Jobling P, Brichta A, Callister R, 'Electrical maturation of sensorimotor processing in the human foetus', JOURNAL OF NEUROCHEMISTRY, Cairns, AUSTRALIA (2015) [E3]
Co-authors Alan Brichta, Phillip Jobling, Rebecca Lim, Robert Callister
2014 Lim R, Drury HR, Camp AJ, Tadros MA, Callister RJ, Brichta AM, 'Anatomical and physiological characterisation of human vestibular hair cells', Journal of Vestibular Research: Equilibrium and Orientation: an international journal of experimental and clinical vestibular science, Buenos Aires, Argentina (2014) [E3]
Co-authors Robert Callister, Alan Brichta, Rebecca Lim
2013 Tadros MA, Fuglevand AJ, Brichta AM, Callister RJ, 'Electrophysiological properties of cranial and spinal motor neurons in mice', Proceedings of the Australian Neuroscience Society, Melbourne (2013) [E3]
Co-authors Alan Brichta, Robert Callister
2012 Zouikr I, Tadros MA, Callister RJ, Nakamura T, Beagley K, Clifton V, Hodgson DM, 'Neonatal lipopolysaccharide exposure alters nociception', Abstracts of the 21st Annual Meeting of the International Behavioral Neuroscience Society, Kona, Hawaii (2012) [E3]
Co-authors Robert Callister, Deborah Hodgson
2012 Tadros MA, Lim R, Graham BA, Hughes DI, Brichta AM, Callister RJ, 'Excitability of human ventral horn neurons during early foetal development', Abstracts. Australian Neuroscience Society 32nd Annual Meeting, Gold Coast, Queensland (2012) [E3]
Co-authors Rebecca Lim, Brett Graham, Alan Brichta, Robert Callister
2012 Zouikr I, Tadros MA, Callister RJ, Nakamura T, Beagley K, Hodgson DM, 'Long-term impact of neonatal exposure to a bacterial mimetic on nociception', Abstracts. Australian Neuroscience Society 32nd Annual Meeting, Gold Coast, Queensland (2012) [E3]
Co-authors Robert Callister, Deborah Hodgson
2012 Lim R, Camp AJ, Tadros MA, Drury HR, Callister RJ, Brichta AM, 'Whole cell conductances of developing human hair cells', Abstracts. Australian Neuroscience Society 32nd Annual Meeting, Gold Coast, Queensland (2012) [E3]
Co-authors Robert Callister, Alan Brichta, Rebecca Lim
2012 Lim R, Camp AJ, Drury HR, Tadros MA, Callister RJ, Brichta AM, 'Characterisation of developing human hair cells', Association for Research in Otolaryngology, San Diego, California (2012)
Co-authors Rebecca Lim, Alan Brichta, Robert Callister
2012 Tadros MA, Jack R, Lim R, Graham BA, Brichta AM, Hughes DI, Callister RJ, 'Sensorimotor processing in the spinal cord of the developing human fetus', Society for Neuroscience, New Orleans, USA (2012)
Co-authors Rebecca Lim, Robert Callister, Alan Brichta, Brett Graham
2012 Lim R, Camp AJ, Tadros MA, Drury HR, Callister RJ, Brichta AM, 'Whole cell conductances of developing human hair cells. Mon 047', 32nd Proceedings of the Australian Neuroscience Society, Gold Coast, Queensland (2012)
Co-authors Rebecca Lim, Alan Brichta, Robert Callister
2011 Tadros MA, Lim R, Graham BA, Hughes DI, Brichta AM, Callister RJ, 'Excitability of human ventral horn neurons during early foetal development', Poster Abstracts. Australian Neuroscience Society Annual Meeting, Auckland, NZ (2011) [E3]
Co-authors Rebecca Lim, Robert Callister, Alan Brichta, Brett Graham
2009 Tadros MA, Anderson WB, Graham BA, Callister RJ, 'Responses to current injection differ between mouse cervical, thoracic and lumbar superficial dorsal horn neurons', ANS 2009 Abstracts: Posters, Canberra, ACT (2009) [E3]
Co-authors Robert Callister, Brett Graham
Show 9 more conferences
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Grants and Funding

Summary

Number of grants 8
Total funding $69,231

Click on a grant title below to expand the full details for that specific grant.


20182 grants / $9,081

Immune infiltration of the Gut-Brain-Axis following neonatal inflammation$6,990

Funding body: Faculty of Science and Information Technology The University of Newcastle

Funding body Faculty of Science and Information Technology The University of Newcastle
Scheme Faculty Strategic Seed Grant
Role Investigator
Funding Start 2018
Funding Finish 2018
GNo
Type Of Funding Internal
Category INTE
UON N

Faculty of Health and Medicine Publication Support $2,091

Funding body: Faculty of Health and Medicine, University of Newcastle

Funding body Faculty of Health and Medicine, University of Newcastle
Scheme Faculty Grant
Role Lead
Funding Start 2018
Funding Finish 2018
GNo
Type Of Funding Internal
Category INTE
UON N

20171 grants / $1,000

Travel Grant$1,000

Funding body: Priority Research Centre for Brain and Mental Health Research (CBMHR)

Funding body Priority Research Centre for Brain and Mental Health Research (CBMHR)
Scheme Travel Grant
Role Lead
Funding Start 2017
Funding Finish 2017
GNo
Type Of Funding Internal
Category INTE
UON N

20153 grants / $54,650

Development of a laboratory model to study adult motoneurons$36,650

Funding body: Keith Tulloch Wine

Funding body Keith Tulloch Wine
Project Team Doctor Melissa Tadros, Professor Robert Callister
Scheme Research Funding
Role Lead
Funding Start 2015
Funding Finish 2015
GNo G1501432
Type Of Funding C3120 - Aust Philanthropy
Category 3120
UON Y

Equipment Grant$15,000

Funding body: Priority Research Centre for Translational Neuroscience & Mental Health

Funding body Priority Research Centre for Translational Neuroscience & Mental Health
Scheme Infrastructure Grant
Role Investigator
Funding Start 2015
Funding Finish 2015
GNo
Type Of Funding Internal
Category INTE
UON N

Modelling sensory neuron development in the human$3,000

Funding body: Faculty of Health and Medicine Pilot Grant University of Newcastle

Funding body Faculty of Health and Medicine Pilot Grant University of Newcastle
Scheme UON Faculty of Health and Medicine Pilot Grant
Role Lead
Funding Start 2015
Funding Finish 2015
GNo
Type Of Funding Internal
Category INTE
UON N

20142 grants / $4,500

Society for Neuroscience Chapter Award$3,000

Funding body: Society for Neuroscience

Funding body Society for Neuroscience
Scheme Chapter Travel Award
Role Lead
Funding Start 2014
Funding Finish 2014
GNo
Type Of Funding External
Category EXTE
UON N

Society for Neuroscience, Washington DC USA, 15 - 19 November 2014$1,500

Funding body: University of Newcastle - Faculty of Health and Medicine

Funding body University of Newcastle - Faculty of Health and Medicine
Project Team Doctor Melissa Tadros
Scheme Travel Grant
Role Lead
Funding Start 2014
Funding Finish 2014
GNo G1400718
Type Of Funding Internal
Category INTE
UON Y
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Dr Melissa Tadros

Positions

Lecturer
School of Biomedical Sciences and Pharmacy
Faculty of Health and Medicine

Research Associate
School of Biomedical Sciences and Pharmacy
Faculty of Health and Medicine

Casual Lecturer
School of Biomedical Sciences and Pharmacy
Faculty of Health and Medicine

Casual Lecturer
School of Biomedical Sciences and Pharmacy
Faculty of Health and Medicine

Contact Details

Email melissa.tadros@newcastle.edu.au
Phone (02) 4921 5609

Office

Room MSB 3.12
Building Medical Sciences Building
Location Callaghan
University Drive
Callaghan, NSW 2308
Australia
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