2023 |
Groen K, Steffens Reinhardt L, Bourdon J-C, Avery-Kiejda KA, 'It is not all about the alpha: elevated expression of p53ß variants is associated with lower probability of survival in a retrospective melanoma cohort.', Cancer Cell Int, 23 228 (2023) [C1]
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Nova |
2023 |
Steffens Reinhardt L, Groen K, Newton C, Avery-Kiejda KA, 'The role of truncated p53 isoforms in the DNA damage response.', Biochim Biophys Acta Rev Cancer, 1878 188882 (2023) [C1]
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Nova |
2023 |
Steffens Reinhardt L, Groen K, Xavier A, Avery-Kiejda KA, 'p53 Dysregulation in Breast Cancer: Insights on Mutations in the TP53 Network and p53 Isoform Expression', International Journal of Molecular Sciences, 24 10078-10078 [C1]
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Nova |
2023 |
Fisch J, de Moura AC, Feistauer V, Reinhardt LS, Molz P, Morás AM, et al., 'Effects of different maternal diets on adipose tissue inflammation and liver tissue oxidative stress in dams and their female offspring', Molecular and Cellular Biochemistry, [C1]
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2023 |
Jacques CED, Lopes FF, Poletto E, Vera LNP, Vianna P, Reinhardt LS, et al., 'Evaluation of oxidative stress and mitochondrial function in a type II mucopolysaccharidosis cellular model: in vitro effects of genistein and coenzyme Q10', Metabolic Brain Disease, 38 519-529 (2023) [C1]
Mucopolysaccharidosis type II (MPS II or Hunter Syndrome) is a lysosomal disease caused by deficient degradation of glycosaminoglycans (GAGs) heparan sulfate and dermatan sulfate ... [more]
Mucopolysaccharidosis type II (MPS II or Hunter Syndrome) is a lysosomal disease caused by deficient degradation of glycosaminoglycans (GAGs) heparan sulfate and dermatan sulfate due to the deficiency of the enzyme iduronate-2-sulfatase. The main treatment for MPS II is the administration of the recombinant form of the enzyme, in a process known as enzyme replacement therapy (ERT). Oxidative damage can contribute to the pathophysiology of MPS II and treatment with ERT can reduce the effects of oxidative stress. For a better understanding of pathophysiology of MPS II, we evaluated biomarkers of mitochondrial dysfunction, DNA (Deoxyribonucleic acid) damage, antioxidant defenses, reactive species production and lysosomal size in IDS-deficient HEK 293 cells and investigate the in vitro effect of genistein and coenzyme Q10 (CoQ) on these biomarkers. An increase in the production of reactive species was demonstrated, as well as an increase in the activities of the antioxidant enzymes superoxide dismutase (SOD) and catalase (CAT). Also, an increase in lysosomal volume and oxidative damage to DNA were verified. There was no evidence of a change in mitochondrial function in this cell model. In the HEK 293 (human embryonic kidney 293) knockout (KO) HP10 cell model we found that genistein at concentrations of 25 and 50¿µm decreased in vitro the production of reactive species and the activity of the SOD enzyme, showing an antioxidant protective effect. Still, in these cells we verified that the coenzyme Q10 in the concentrations of 5 and 10¿µm decreased in vitro the activity of the SOD enzyme and in the concentration of 10¿µm decreased in vitro the DNA damage, also demonstrating antioxidant protection. In conclusion, MPS II knockout cells demonstrated oxidative stress and DNA damage and genistein, as well as coenzyme Q10, have been shown to have an important protective effect in vitro against these oxidative damages.
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Nova |
2023 |
Henn JG, Bernardes Ferro M, Lopes Alves GA, Pires Peña F, de Oliveira JVR, de Souza BM, et al., 'Development and characterization of a temozolomide-loaded nanoemulsion and the effect of ferrocene pre and co-treatments in glioblastoma cell models', Pharmacological Reports, 75 1597-1609 (2023) [C1]
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2023 |
Hammerschmidt TG, Donida B, Raabe M, Faverzani JL, Lopes FDF, Machado AZ, et al., 'Evidence of redox imbalance and mitochondrial dysfunction in Niemann-Pick type C1 patients: the
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Nova |
2023 |
Steffens Reinhardt L, Groen K, Zhang X, Morten BC, Wawruszak A, Avery-Kiejda KA, 'p53 isoform expression promotes a stemness phenotype and inhibits doxorubicin sensitivity in breast cancer.', Cell Death Dis, 14 509 (2023) [C1]
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Nova |
2022 |
Steffens Reinhardt L, Groen K, Morten BC, Bourdon J-C, Avery-Kiejda KA, 'Cytoplasmic p53ß Isoforms Are Associated with Worse Disease-Free Survival in Breast Cancer.', Int J Mol Sci, 23 (2022) [C1]
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Nova |
2022 |
Hammerschmidt TG, Donida B, Faverzani JL, Moura AP, dos Reis BG, Machado AZ, et al., 'Cytokine profile and cholesterol levels in patients with Niemann-Pick type C disease presenting neurological symptoms: in vivo effect of miglustat and in vitro effect of N-acetylcysteine and coenzyme Q10', Experimental Cell Research, 416 (2022) [C1]
Niemann Pick type C is an inborn error of metabolism (IEM), classified as a lysosomal storage disease (LSD) caused by a dysfunction in NPC transport protein, that leads to intrace... [more]
Niemann Pick type C is an inborn error of metabolism (IEM), classified as a lysosomal storage disease (LSD) caused by a dysfunction in NPC transport protein, that leads to intracellular accumulation of non-esterified cholesterol and other lipids. Clinical manifestations are ample, with visceral and neurological symptoms. Miglustat, a molecule that reversibly inhibits glucosylceramide synthase is used as treatment for this disorder. Studies demonstrated the influence of oxidative stress and inflammation in IEM, as well in animal model of NP-C disease. Nonetheless, literature lacks data on patients, so our work aimed to investigate if there is influence of chronic inflammation in the pathophysiology of NP-C disease, and the effect of miglustat, N-acetylcysteine (NAC) and Coenzyme Q10 (CoQ10). We evaluated the plasmatic cytokines in NPC patients at diagnosis and during the treatment with miglustat. Additionally, we performed an in vitro study with antioxidants NAC (1 mM and 2.5 mM) and CoQ10 (5 µM and 10 µM), where we could verify its effect on inflammatory parameters, as well as in cholesterol accumulation. Our results showed that NP-C patients have higher plasmatic levels of pro and anti-inflammatory cytokines (IL-6, IL-8, and IL-10) at diagnosis and the treatment with miglustat was able to restore it. In vitro study showed that treatment with antioxidants in higher concentrations significantly decrease cholesterol accumulation, and NAC at 2.5 mM normalized the level of pro-inflammatory cytokines. Although the mechanism is not completely clear, it can be related to restoration in lipid traffic and decrease in oxidative stress caused by antioxidants.
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Nova |
2022 |
Gauthier MF, de Andrade AA, Fisch J, Feistauer V, Morás AM, Reinhardt LS, et al., 'Dietary interventions in mice affect oxidative stress and gene expression of the Prlr and Esr1 in the adipose tissue and hypothalamus of dams and their offspring', Journal of Physiology and Biochemistry, 78 271-282 (2022) [C1]
Maternal diet is key to the progeny¿s health since it may impact on the offspring¿s adult life. In this study, mice dams received standard (CONT), restrictive (RD), or hypercalori... [more]
Maternal diet is key to the progeny¿s health since it may impact on the offspring¿s adult life. In this study, mice dams received standard (CONT), restrictive (RD), or hypercaloric (HD) diets during mating, pregnancy, and lactation. Male offspring of each group of dams also received these diets: CONT, RD, HD. Aiming to evaluate the oxidative stress in the adipose tissue, reactive oxygen species (ROS) production, catalase (CAT), and superoxide dismutase (SOD) activities were analyzed in dams and offspring. In the adipose tissue and hypothalamus, gene expression of prolactin (Prlr) and estrogen alpha (Esr1) receptors was performed in dams and offspring. Protein expression of Stat5 was evaluated in the adipose tissue of the offspring from RD-fed dams. HD-fed dams increased triglycerides and leptin serum concentrations, and decreased SOD activity in the adipose tissue. In the offspring¿s adipose tissue, we observed a maternal diet effect caused by HD, with increased ROS production and SOD and CAT activities. Gene expression of Prlr and Esr1 in the offspring¿s adipose tissue was decreased due to maternal RD. Mice from HD-fed dams showed higher Stat5 expression compared to the offspring from CONT and RD dams in the adipose tissue. In the hypothalamus, we found decreased expression of Prlr in RD and HD dams, compared to CONT; and a maternal diet effect on Prlr and Esr1 gene expression in the offspring. In conclusion, we can affirm that maternal nutrition impacts the redox state and influences the gene expression of Prlr and Esr1, which are involved in energy metabolism, both peripherally and centrally in the adult life of the female offspring.
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2022 |
Zhang X, Groen K, Morten BC, Reinhardt LS, Campbell HG, Braithwaite AW, et al., 'Effect of p53 and its N-terminally truncated isoform, 40p53, on breast cancer migration and invasion', MOLECULAR ONCOLOGY, 16 447-465 (2022) [C1]
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Nova |
2022 |
Reinhardt LS, Moras AM, Henn JG, Arantes PR, Ferro MB, Braganhol E, et al., 'Nek1-inhibitor and temozolomide-loaded microfibers as a co-therapy strategy for glioblastoma treatment', INTERNATIONAL JOURNAL OF PHARMACEUTICS, 617 (2022) [C1]
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2022 |
Reinhardt LS, Zhang X, Groen K, Morten BC, De Iuliis GN, Braithwaite AW, et al., 'Alterations in the p53 isoform ratio govern breast cancer cell fate in response to DNA damage', CELL DEATH & DISEASE, 13 (2022) [C1]
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Nova |
2021 |
Reinhardt LS, Henn JG, Moras AM, de Moura Sperotto ND, Ferro MB, Cao Z, et al., 'Plantago australis Hydroethanolic Extract-Loaded Formulations: Promising Dressings for Wound Healing', REVISTA BRASILEIRA DE FARMACOGNOSIA-BRAZILIAN JOURNAL OF PHARMACOGNOSY, 31 91-101 (2021) [C1]
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2021 |
Moras AM, Henn JG, Reinhardt LS, Lenz G, Moura DJ, 'Recent developments in drug delivery strategies for targeting DNA damage response in glioblastoma', LIFE SCIENCES, 287 (2021) [C1]
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2021 |
Faverzani JL, Steinmetz A, Deon M, Marchetti DP, Guerreiro G, Sitta A, et al., 'L-carnitine protects DNA oxidative damage induced by phenylalanine and its keto acid derivatives in neural cells: a possible pathomechanism and adjuvant therapy for brain injury in phenylketonuria', METABOLIC BRAIN DISEASE, 36 1957-1968 (2021) [C1]
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2021 |
Seba V, de Lima GG, Pereira BL, Silva G, Reinhardt LS, Arantes PR, et al., 'Development, Characterization and Cell Viability Inhibition of PVA Spheres Loaded with Doxorubicin and 4'-Amino-1-Naphthyl-Chalcone (D14) for Osteosarcoma', POLYMERS, 13 (2021) [C1]
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Nova |
2020 |
Steffens Reinhardt L, Zhang X, Wawruszak A, Groen K, De Iuliis GN, Avery-Kiejda KA, 'Good Cop, Bad Cop: Defining the Roles of 40p53 in Cancer and Aging', Cancers, 12 (2020) [C1]
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Nova |
2020 |
Zancan M, Malysz T, Moura DJ, Morás AM, Steffens L, Rasia-Filho AA, 'Gap junctions and expression of Cx36, Cx43 and Cx45 in the posterodorsal medial amygdala of adult rats.', Histol Histopathol, 35 395-403 (2020)
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2020 |
Ana Moira M, Luiza S, Bruna Eliza N, Jenifer S, Eliane D, Luciana Grazziotin R-G, Dinara Jaqueline M, 'Cytotoxic mechanism of Bothrops jararaca venom mediated by mitochondrial depolarization', Advances in Toxicology and Toxic Effects, 4 001-008 (2020) [C1]
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2020 |
Zancan M, Moura DJ, Morás AM, Steffens L, de Moura AC, Giovenardi M, Rasia-Filho AA, 'Neurotrophic factors in the posterodorsal medial amygdala of male and cycling female rats.', Brain Res Bull, 155 92-101 (2020)
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2020 |
Steffens L, Morás AM, Arantes PR, Masterson K, Cao Z, Nugent M, Moura DJ, 'Electrospun PVA-Dacarbazine nanofibers as a novel nano brain-implant for treatment of glioblastoma: in silico and in vitro characterization.', Eur J Pharm Sci, 143 105183 (2020)
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2020 |
Donida B, Raabe M, Tauffner B, de Farias MA, Machado AZ, Timm F, et al., 'Nanoparticles containing ß-cyclodextrin potentially useful for the treatment of Niemann-Pick C.', J Inherit Metab Dis, 43 586-601 (2020)
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2019 |
Steffens Reinhardt L, Chee BS, Cao Z, Moura DJ, Nugent M, 'Freeze-thaw electrospun PVA-dacarbazine nanoparticles: preparation, characterization and anticancer evaluation', INTERNATIONAL JOURNAL OF POLYMERIC MATERIALS AND POLYMERIC BIOMATERIALS, 69 749-760 (2019)
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2019 |
Henn JG, Steffens L, de Moura Sperotto ND, de Souza Ponce B, Veríssimo RM, Boaretto FBM, et al., 'Toxicological evaluation of a standardized hydroethanolic extract from leaves of Plantago australis and its major compound, verbascoside.', J Ethnopharmacol, 229 145-156 (2019)
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2019 |
Luft JG, Steffens L, Morás AM, da Rosa MS, Leipnitz G, Regner GG, et al., 'Rosmarinic acid improves oxidative stress parameters and mitochondrial respiratory chain activity following 4-aminopyridine and picrotoxin-induced seizure in mice.', Naunyn Schmiedebergs Arch Pharmacol, 392 1347-1358 (2019)
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2019 |
Hauschild TC, Guerreiro G, Mescka CP, Coelho DM, Steffens L, Moura DJ, et al., 'DNA damage induced by alloisoleucine and other metabolites in maple syrup urine disease and protective effect of l-carnitine.', Toxicol In Vitro, 57 194-202 (2019)
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2018 |
Caletti G, Herrmann AP, Pulcinelli RR, Steffens L, Morás AM, Vianna P, et al., 'Taurine counteracts the neurotoxic effects of streptozotocin-induced diabetes in rats.', Amino Acids, 50 95-104 (2018)
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2018 |
Marchetti DP, Steffens L, Jacques CE, Guerreiro GB, Mescka CP, Deon M, et al., 'Oxidative Imbalance, Nitrative Stress, and Inflammation in C6 Glial Cells Exposed to Hexacosanoic Acid: Protective Effect of N-acetyl-L-cysteine, Trolox, and Rosuvastatin.', Cell Mol Neurobiol, 38 1505-1516 (2018)
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2018 |
Steinmetz A, Steffens L, Morás AM, Prezzi F, Braganhol E, Saffi J, et al., 'In vitro model to study cocaine and its contaminants.', Chem Biol Interact, 285 1-7 (2018)
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2018 |
de Moura Sperotto ND, Steffens L, Veríssimo RM, Henn JG, Péres VF, Vianna P, et al., 'Wound healing and anti-inflammatory activities induced by a Plantago australis hydroethanolic extract standardized in verbascoside.', J Ethnopharmacol, 225 178-188 (2018)
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2018 |
Bohn DR, Lobato FO, Thill AS, Steffens L, Raabe M, Donida B, et al., 'Artificial cerium-based proenzymes confined in lyotropic liquid crystals: synthetic strategy and on-demand activation.', J Mater Chem B, 6 4920-4928 (2018)
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2018 |
Brito da Silva C, Gil ES, da Silveira Santos F, Morás AM, Steffens L, Bruno Gonçalves PF, et al., 'Proton-Transfer-Based Azides with Fluorescence Off-On Response for Detection of Hydrogen Sulfide: An Experimental, Theoretical, and Bioimaging Study.', J Org Chem, 83 15210-15224 (2018)
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2018 |
da Costa E Silva LD, Pereira P, Regner GG, Boaretto FBM, Hoffmann C, Pflüger P, et al., 'DNA damage and oxidative stress induced by seizures are decreased by anticonvulsant and neuroprotective effects of lobeline, a candidate to treat alcoholism.', Metab Brain Dis, 33 53-61 (2018)
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2018 |
Freese L, Almeida FB, Heidrich N, Hansen AW, Steffens L, Steinmetz A, et al., 'Environmental enrichment reduces cocaine neurotoxicity during cocaine-conditioned place preference in male rats.', Pharmacol Biochem Behav, 169 10-15 (2018)
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2017 |
de Souza VP, Vendrusculo V, Moras AM, Steffens L, Santos FS, Moura DJ, et al., 'Synthesis and photophysical study of new fluorescent proton transfer dihydropyrimidinone hybrids as potential candidates for molecular probes', NEW JOURNAL OF CHEMISTRY, 41 15305-15311 (2017)
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2017 |
Borille BT, González M, Steffens Reinhardt L, Ortiz RS, Limberger RP, 'Cannabis sativa: A systematic review of plant analysis', Drug Analytical Research, 1 1-23 (2017) [C1]
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2017 |
Biancini GB, Morás AM, Reinhardt LS, Busatto FF, de Moura Sperotto ND, Saffi J, et al., 'Globotriaosylsphingosine induces oxidative DNA damage in cultured kidney cells.', Nephrology (Carlton), 22 490-493 (2017)
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