Dr Kim Tran

Tank goby Glossogobius giuris is widely distributed across Vietnamese rivers, estuaries, and lagoons, particularly in the central region where this species plays a pivotal role as a high commercial source for local communities. In this study, we employed landmark-based geometric morphometrics to compare the morphometric variations among three distinct populations of G. giuris inhabiting the Tam Giang Lagoon (Thua Thien Hue Province), Nhat Le Estuary (Quang Binh Province), and Truong Giang River (Quang Nam Province), Vietnam. The analysis, utilizing Permutational Multivariate Analysis of Variance (PERMANOVA) with Mahalanobis distances, revealed significant differences in the body shape among the three populations (P < 0.001). Specifically, with respect to the lateral side, the highest Mahalanobis distance was observed between specimens from the Tam Giang and Nhat Le, followed by the Tam Giang and Truong Giang River, with the lowest value found between the Nhat Le and Truong Giang. A similar pattern was observed for the dorsal side. Canonical variate analysis (CVA) illustrated three distinct groups with statistical significance in all cases (P < 0.001) and the confusion matrix showed a high corrected grouping rate of 88.5% for the lateral side and 82.8% for the dorsal side. These findings indicated notable variations in the body shape of G. giuris among the three studied areas. Fish sampled from the Tam Giang Lagoon exhibited a streamlined body shape, while those from the Truong Giang River and Nhat Le Estuary displayed a deeper body profile. The observed morphological differences among these fish populations are likely attributed to phenotypic adaptations driven by environmental factors and habitat distinctions. Importantly, this study marks the first attempt to compare the body shape variations of G. giuris in both freshwater and estuarine environments within central Vietnam, utilizing a landmark-based geometric approach. These findings provide crucial insights for further investigations into the ecological adaptations and evolutionary processes of this fish species.

Mesoporous silica-based materials are currently being explored as a new type of bioscaffold for bone regeneration applications. Zinc(Zn)¿ion incorporation is shown to play an important role in promoting bone regeneration and also providing antimicrobial activity to the scaffold materials. In this work, the role of pore size, geometry, and ordered structure on the Zn loading and release performance of two different mesoporous silica, SBA-1 and SBA-15, are compared. Zn loading is varied from 2.5 to 10 wt% for both samples, and its effect on the antibacterial and osteogenic activity is evaluated. Zn loading up to 10 wt% has a negligible effect on the morphology and textural properties of the mesoporous silica samples. The inductively coupled plasma mass spectrometry (ICP-MS) analysis reveals that SBA-15 exhibits significantly higher Zn release in Luria-Bertani (LB) broth as compared to SBA-1 that is reflected in the higher antibacterial activity of SBA-15 against both gram-positive and gram-negative bacteria. Various assays show that 5 wt% Zn loading is sufficient to produce both bactericidal and inhibitory effects on bacterial cells. The 5 wt% Zn-loaded samples induce osteogenic differentiation ofavianized bone marow-derived stromal cells (TVA-BMSCs) though SBA-15 samples show better compatibility compared to SBA-1, suggesting that Zn incorporation can produce sufficient antibacterial effect and osteogenic differentiation of TVA-BMSCs.

Oyster aquaculture in northern Vietnam is a new and growing industry that brings benefits to coastal communities. By understanding the oyster supply chain, the potential value can be fully explored, and socio-economic and environmental gains targeted. This study surveyed industry representatives ¿ from farm to plate ¿ to examine the industry status, distribution network, challenges, and opportunities. Four broad distribution network models were identified: direct-to-sale, wholesaler, cooperative/processor, and retailer. These models are quite different and comparing them is complex. A reduction in the marketing margin in any supply chain is likely to result in shared economic and/or welfare gains for pre-harvest elements, elements in the value chain, and consumers. Similarly, lower farm production and hatchery costs and increased consumer demand would result in welfare gains shared along the supply chain, although these do not necessarily translate to changes in the marketing margin. Key challenges were associated with spatial and marine development planning, lack of monitoring, collaboration/coordination along the supply chain, market price variation and consumers. Challenges were further explored in the categories of collaboration/coordination, regulation, and export. Opportunities exist to better understand how oyster shell might be used as a by-product such as a livestock mineral supplement, in soil acidity management, and in crop and vegetable growth. Almost half of the survey respondents have oyster shell as a by-product, suggesting the potential for carbon offset schemes could also be explored, at least to the extent to which they ameliorate the carbon footprint of the industry. These opportunities could advance and value-add to oyster aquaculture in northern Vietnam.

This study aimed to reveal possible alterations to lipidomic profiles in Sydney rock oysters, Saccostrea glomerata, exposed to estrogenic mixtures (i.e., estrone, E1; 17ß-estradiol, E2; estriol, E3; 17a-ethinylestradiol, EE2; bisphenol A, BPA; 4-t-octylphenol, 4-t-OP; and 4-nonylphenol, 4-NP) at "low" and "high" concentrations, typical of those detected in Australian and global receiving waters. A seven-day acute exposure window exhibited significantly lower abundances of many non-polar metabolites in digestive gland, gills, and gonads. Overall, there was a strong effect of the carrier solvent ethanol (despite a low exposure of 0.0002%), with all solvent containing treatments exhibiting lower abundances of lipidic metabolites, especially in the gill and digestive gland. No significant changes of the lipidome were exhibited in the male gonad by estrogenic exposure. However, in the female gonad, significant reductions of phospholipids and phosphatidylcholine were associated with exposure to high estrogenic mixtures. We hypothesise that the decreases in these phospholipids in the female gonad may be attributable to 1) lower algal consumption and thus lower uptake of lipidic building blocks; 2) a reduction of available substrates for phospholipid and phosphatidylcholine synthesis; and/or 3) induction of reactive oxygen species via estrogen metabolism, which may cause lipid peroxidation and lower abundance of phospholipids.

This study assessed the ability of phosphorus (P) fertilizer to remediate the rhizosphere of three wild plant species (Banksia seminuda, a tree; Chloris truncata, a grass; and Hakea prostrata, a shrub) growing in a soil contaminated with total (aliphatic) petroleum hydrocarbon (TPH). Plant growth, photosynthesis (via chlorophyll fluorescence), soil microbial activity, alkane hydroxylase AlkB (aliphatic hydrocarbon-degrading) gene abundance, and TPH removal were evaluated 120 days after planting. Overall, although TPH served as an additional carbon source for soil microorganisms, the presence of TPH in soil resulted in decreased plant growth and photosynthesis. However, growth, photosynthesis, microbial activities, and AlkB gene abundance were enhanced by the application of P fertilizer, thereby increasing TPH removal rates, although the extent and optimum P dosage varied among the plant species. The highest TPH removal (64.66%) was observed in soil planted with the Poaceae species, C. truncata, and amended with 100 mg P kg-1 soil, while H. prostrata showed higher TPH removal compared to the plant belonging to the same Proteaceae family, B. seminuda. The presence of plants resulted in higher AlkB gene abundance and TPH removal relative to the unplanted control. The removal of TPH was associated directly with AlkB gene abundance (R2 > 0.9, p < 0.001), which was affected by plant identity and P levels. The results indicated that an integrated approach involving wild plant species and optimum P amendment, which was determined through experimentation using different plant species, was an efficient way to remediate soil contaminated with TPH.

This study assessed the appropriate sampling design required for quantifying variability in metal accumulation in the leaf tissues of A. marina, a dominant mangrove inhabiting Australian estuaries, by applying a hierarchical nested sampling design to sample mangroves at various levels of biological and spatial hierarchies (leaf, branch, tree, site). It was revealed that most variation in metal accumulation occurred among trees and branches, with insignificant variation between sites and among leaves. We also examined the accumulation of metal (loid)s in the leaf tissues collected from six locations across the Georges River estuary in southern Sydney, which differ in metal contamination history. Prospect Creek and Salt Pan Creek were the most contaminated locations, which exceeded sediment quality guideline values for Cu (66.71 ± 2.18 µg/g), Zn (317.14 ± 46.14 µg/g) and Pb (81.02 ± 2.79 µg/g). All metal(loid) concentrations in leaf tissues were much lower than their concentrations in sediment, but essential metals exhibited greater mobility. Out of 10 metal(loid)s, Mn, Co and Pb in leaves showed linear relationships (R2 = 0.28¿0.47) with sediment, indicating that mangrove leaves may be used as a bioindicator of environmental loads for these metals.

The current study represents the first attempt to analyse quantitatively, within a phylogenetic framework, uptake and partitioning patterns of copper (Cu), zinc (Zn), cadmium (Cd) and lead (Pb) in extant saltmarsh taxa globally, and to assess associations of these patterns with various plant traits indicative of their halophytic adaptations. Despite saltmarsh being diverse taxonomically, most saltmarsh taxa accumulate metals to roots at, or above, unity (> 1). Further, there is significant translocation from roots to shoot for Cu, Zn and Cd (= 1), however, Pb is less mobile (TF = 0.65). Patterns of accumulation were similar among families, except greater Cd accumulation to roots in members of Juncaceae. Patterns of uptake to roots and translocation to leaves were broadly similar among plant type, plant form, habitat and photosynthetic mode. Zinc is lower in the leaves of salt-secreting species for some closely related taxa, suggesting some species co-excrete sodium (Na+) and Zn2+ through glands in leaf tissue. Salinity tolerance has no relationship to metal uptake and translocation. Translocation of Zn is greater at lower Zn sediment exposures, reflecting its active uptake and essentiality, but such bias does not affect outcomes of analyses when included as a covariate.

Microplastics (MPs) exposed to the natural environment provide an ideal surface for biofilm formation, which potentially acts as a reactive phase facilitating the sorption of hazardous contaminants. Until now, changes in the contaminant sorption capacity of MPs due to biofilm formation have not been quantified. This is the first study that compared the capacity of naturally aged, biofilm-covered microplastic fibers (BMFs) to adsorb perfluorooctane sulfonate (PFOS) and lead (Pb) at environmentally relevant concentrations. Changes in the surface properties and morphology of aged microplastic fibers (MF) were studied by surface area analysis, infrared spectroscopy, and scanning electron microscopy. Results revealed that aged MFs exhibited higher surface areas because of biomass accumulation compared to virgin samples and followed the order polypropylene>polyethylene>nylon>polyester. The concentrations of adsorbed Pb and PFOS were 4-25% and 20-85% higher in aged MFs and varied among the polymer types. The increased contaminant adsorption was linked with the altered surface area and the hydrophobic/hydrophilic characteristics of the samples. Overall, the present study demonstrates that biofilms play a decisive role in contaminant-plastic interactions and significantly enhance the vector potential of MFs for toxic environmental contaminants. We anticipate that knowledge generated from this study will help refine the planetary risk assessment of MPs.

The long-term aging of plastic leads to weathering and biofouling that can influence the behavior and fate of plastic in the marine environment. This is the first study to fingerprint the contaminant profiles and bacterial communities present in plastic-associated inorganic and organic matter (PIOM) isolated from 10 year-aged plastic. Plastic sleeves were sampled from an oyster aquaculture farm and the PIOM was isolated from the intertidal, subtidal, and sediment-buried segments to investigate the levels of metal(loid)s, polyaromatic hydrocarbons (PAHs), per-fluoroalkyl substances (PFAS) and explore the microbial community composition. Results indicated that the PIOM present on long-term aged high-density polyethylene plastic harbored high concentrations of metal(loid)s, PAHs, and PFAS. Metagenomic analysis revealed that the bacterial composition in the PIOM differed by habitat type, which consisted of potentially pathogenic taxa including Vibrio, Shewanella, and Psychrobacter. This study provides new insights into PIOM as a potential sink for hazardous environmental contaminants and its role in enhancing the vector potential of plastic. Therefore, we recommend the inclusion of PIOM analysis in current biomonitoring regimes and that plastics be used with caution in aquaculture settings to safeguard valuable food resources, particularly in areas of point-source contamination.

Estrogenic compounds enter waterways via effluents from wastewater treatment works (WWTW), thereby indicating a potential risk to organisms inhabiting adjacent receiving waters. However, little is known about the loads or concentrations of estrogenic compounds that enter Australian WWTWs, the efficiency of removing estrogenic compounds throughout the various stages of tertiary WWTW processes (which are common in Australia), nor the concentrations released into estuarine or marine receiving waters, and the associated risk for aquatic taxa residing in these environments. Therefore, seven estrogenic compounds, comprising the natural estrogens estrone (E1), 17ß-estradiol (E2) and estriol (E3), the synthetic estrogen (EE2), and the industrial chemicals bisphenol A (BPA), 4-t-octyl phenol (4-t-OP) and 4-nonyl phenol (4-NP), in wastewater samples were quantified via liquid chromatographic-mass spectrometry (LC-MS) after solid-phase extraction at different stages of wastewater treatment and associated receiving waters. The concentrations of the target compounds in wastewater ranged from < LOQ (limit of quantification) to 158 ng/L for Tanilba Bay WWTW and < LOQ to 162 ng/L for Belmont WWTW. Most target compounds significantly declined after the secondary treatment phase. Appreciable removal efficiency throughout the treatment process was observed with removal from 39.21 to 99.98% of influent values at both WWTWs. The reduction of the natural estrogens (E1, E2 and E3) and 4-t-OP were significantly greater than EE2, BPA, and 4-NP in both WWTWs. Risk quotients (RQs) were calculated to assess potential ecological risks from individual estrogenic compounds. In predicted diluted effluents, no targeted compounds showed any ecological risk (RQ =1.65 × 10-2) at both WWTWs. Similarly, all RQs for shore samples at both WWTWs were below 1. Finally, the hazard index (HI), which represents combined estrogenic contaminants' ecological risk, indicated no mentionable risk for predicted diluted effluents (HI = 0.0097 to 0.0218) as well as shoreline samples (HI = 0.393 to 0.522) in the receiving estuarine or marine waters.

We examined the patterns of uptake and partitioning of metal(loid)s in Suaeda australis from three highly urbanised estuaries (Sydney Olympic Park, Hunter Wetlands and Lake Macquarie) in NSW, Australia. Of these, Sydney Olympic Park was found to be the most contaminated estuary in terms of combined sediment metal(loid) load, followed by Hunter Wetlands and lowest in Lake Macquarie (via PERMANOVA). Uptake in roots was greater for the essential metals Cu and Zn along with the non-essential metal Cd and the metalloid Se (root BCFs >1) and lower for Pb and As (root BCFs <1). Substantial barriers for translocation from roots to stems were identified for all metal(loid)s (stem TFs; 0.07¿0.68). Conversely, unrestricted flow from stems to leaves was observed for all metal(loid)s at unity or higher (leaf TFs = 1). Strong linear relationships between sediment and root for Zn and Pb were observed, indicating roots as a useful bioindicator.

Marine molluscs, such as oysters, respond to estrogenic compounds with the induction of the egg yolk protein precursor, vitellogenin (Vtg), availing a biomarker for estrogenic pollution. Despite this application, the precise molecular mechanism through which estrogens exert their action to induce molluscan vitellogenesis is unknown. As a first step to address this question, we cloned a gene encoding Vtg from the Sydney rock oyster Saccostrea glomerata (sgVtg). Using primers designed from a partial sgVtg cDNA sequence available in Genbank, a full-length sgVtg cDNA of 8498 bp was obtained by 5'- and 3'-RACE. The open reading frame (ORF) of sgVtg was determined to be 7980 bp, which is substantially longer than the orthologs of other oyster species. Its deduced protein sequence shares the highest homology at the N- and C-terminal regions with other molluscan Vtgs. The full-length genomic DNA sequence of sgVtg was obtained by genomic PCR and genome walking targeting the gene body and flanking regions, respectively. The genomic sequence spans 20 kb and consists of 30 exons and 29 introns. Computer analysis identified three closely spaced half-estrogen responsive elements (EREs) in the promoter region and a 210-bp CpG island 62 bp downstream of the transcription start site. Upregulation of sgVtg mRNA expression was observed in the ovaries following in vitro (explants) and in vivo (tank) exposure to 17ß-estradiol (E2). Notably, treatment with an estrogen receptor (ER) antagonist in vitro abolished the upregulation, suggesting a requirement for an estrogen-dependent receptor for transcriptional activation. DNA methylation of the 5' CpG island was analysed using bisulfite genomic sequencing of the in vivo exposed ovaries. The CpG island was found to be hypomethylated (with 0-3% methylcytosines) in both control and E2-exposed oysters. However, no significant differential methylation or any correlation between methylation and sgVtg expression levels was observed. Overall, the results support the possible involvement of an ERE-containing promoter and an estrogen-activated receptor in estrogen signalling in marine molluscs.