Dr Thi Thu Suong Nguyen

Postdoctoral Researcher

School of Environmental and Life Sciences

Career Summary

Biography

Suong Nguyen earned her B.S. in Biotechnology (2007) and M.S. in Genetics (2010) from the University of Natural Sciences, HCM, Vietnam. She then commenced her PhD study in the laboratory of Assoc/Prof. David McCurdy at the University of Newcastle. Her project was to investigate the molecular mechanism underlying the development of transfer cells – a unique cell type specialised for nutrient transport in plants. She completed her PhD in 2017 and continued transfer cell research in the McCurdy Lab as a Research Assistant. In March 2020 she joined the BlueGreen team of Prof. Brett Neilan at UON as a Postdoctoral Research Associate to study cyanobacteria.

Qualifications

  • Doctor of Philosophy, University of Newcastle
  • Master of Science, National University of Civil Engineering - Vietnam

Keywords

  • cyanobacteria, bioinformatics

Languages

  • English (Working)

Fields of Research

Code Description Percentage
060599 Microbiology not elsewhere classified 100

Professional Experience

UON Appointment

Title Organisation / Department
Casual Tutor University of Newcastle
UON Global
Australia
Postdoctoral Researcher University of Newcastle
School of Environmental and Life Sciences
Australia
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Publications

For publications that are currently unpublished or in-press, details are shown in italics.


Chapter (1 outputs)

Year Citation Altmetrics Link
2016 Nguyen STT, McCurdy DW, 'Transdifferentiation: A Plant Perspective', Molecular Cell Biology of the Growth and Differentiation of Plant Cells, CRC Press, Boca Raton, Florida 298-319 (2016) [B1]
DOI 10.1201/b20316-21
Co-authors David Mccurdy

Journal article (5 outputs)

Year Citation Altmetrics Link
2018 Wu Y, Hou J, Yu F, Nguyen STT, McCurdy DW, 'Transcript Profiling Identifies NAC-Domain Genes Involved in Regulating Wall Ingrowth Deposition in Phloem Parenchyma Transfer Cells of Arabidopsis thaliana', FRONTIERS IN PLANT SCIENCE, 9 (2018) [C1]
DOI 10.3389/fpls.2018.00341
Citations Scopus - 4Web of Science - 3
Co-authors David Mccurdy
2017 Nguyen STT, McCurdy DW, 'Wall ingrowth deposition in phloem parenchyma transfer cells in Arabidopsis: Heteroblastic variations and a potential role in pathogen defence', Plant Signaling and Behavior, 12 (2017) [C1]
DOI 10.1080/15592324.2017.1338226
Citations Scopus - 2Web of Science - 1
Co-authors David Mccurdy
2017 Nguyen STT, Greaves T, McCurdy DW, 'Heteroblastic Development of Transfer Cells Is Controlled by the microRNA miR156/SPL Module', PLANT PHYSIOLOGY, 173 1676-1691 (2017) [C1]
DOI 10.1104/pp.16.01741
Citations Scopus - 7Web of Science - 8
Co-authors David Mccurdy
2015 Nguyen STT, McCurdy DW, 'High-resolution confocal imaging of wall ingrowth deposition in plant transfer cells: Semi-quantitative analysis of phloem parenchyma transfer cell development in leaf minor veins of Arabidopsis', BMC Plant Biology, 15 (2015) [C1]

© 2015 Nguyen and McCurdy; licensee BioMed Central. Background: Transfer cells (TCs) are trans-differentiated versions of existing cell types designed to facilitate enhanced membr... [more]

© 2015 Nguyen and McCurdy; licensee BioMed Central. Background: Transfer cells (TCs) are trans-differentiated versions of existing cell types designed to facilitate enhanced membrane transport of nutrients at symplasmic/apoplasmic interfaces. This transport capacity is conferred by intricate wall ingrowths deposited secondarily on the inner face of the primary cell wall, hence promoting the potential trans-membrane flux of solutes and consequently assigning TCs as having key roles in plant growth and productivity. However, TCs are typically positioned deep within tissues and have been studied mostly by electron microscopy. Recent advances in fluorophore labelling of plant cell walls using a modified pseudo-Schiff-propidium iodide (mPS-PI) staining procedure in combination with high-resolution confocal microscopy have allowed visualization of cellular details of individual tissue layers in whole mounts, hence enabling study of tissue and cellular architecture without the need for tissue sectioning. Here we apply a simplified version of the mPS-PI procedure for confocal imaging of cellulose-enriched wall ingrowths in vascular TCs at the whole tissue level. Results: The simplified mPS-PI staining procedure produced high-resolution three-dimensional images of individual cell types in vascular bundles and, importantly, wall ingrowths in phloem parenchyma (PP) TCs in minor veins of Arabidopsis leaves and companion cell TCs in pea. More efficient staining of tissues was obtained by replacing complex clearing procedures with a simple post-fixation bleaching step. We used this modified procedure to survey the presence of PP TCs in other tissues of Arabidopsis including cotyledons, cauline leaves and sepals. This high-resolution imaging enabled us to classify different stages of wall ingrowth development in Arabidopsis leaves, hence enabling semi-quantitative assessment of the extent of wall ingrowth deposition in PP TCs at the whole leaf level. Finally, we conducted a defoliation experiment as an example of using this approach to statistically analyze responses of PP TC development to leaf ablation. Conclusions: Use of a modified mPS-PI staining technique resulted in high-resolution confocal imaging of polarized wall ingrowth deposition in TCs. This technique can be used in place of conventional electron microscopy and opens new possibilities to study mechanisms determining polarized deposition of wall ingrowths and use reverse genetics to identify regulatory genes controlling TC trans-differentiation.

DOI 10.1186/s12870-015-0483-8
Citations Scopus - 9Web of Science - 9
Co-authors David Mccurdy
2013 Chinnappa KSA, Nguyen TTS, Hou J, Wu Y, McCurdy DW, 'Phloem parenchyma transfer cells in Arabidopsis - an experimental system to identify transcriptional regulators of wall ingrowth formation', Frontiers in Plant Science, 4 (2013) [C1]
DOI 10.3389/fpls.2013.00102
Citations Scopus - 17Web of Science - 15
Co-authors David Mccurdy
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Dr Thi Thu Suong Nguyen

Positions

Postdoctoral Researcher
School of Environmental and Life Sciences
Faculty of Science

Casual Tutor
UON Global
Global Engagement and Partnerships Division

Contact Details

Email thithusuong.nguyen@newcastle.edu.au
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