Dr Shafiq Syed
Cancer Institute NSW EC Fellow
School of Biomedical Sciences and Pharmacy
- Email:shafiq.syed@newcastle.edu.au
- Phone:(02) 4921 7280
Career Summary
Biography
Shafiq Syed obtained a Bachelor of Veterinary Medicine and Master of Biotechnology and Immunology from India before moving to the University of Newcastle, Australia, where he obtained his PhD degree in 2018. During his PhD, he discovered, for the first time, the biomarker for the much elusive uterine stem cells and detailed their functional characterisation during uterine homeostasis, regeneration and cancer, which further lead to the identification of the cell of origin for endometrial cancer. Dr Syed published his PhD research as a lead author in Cell Stem Cell. The exceptional quality of this research work was also recognised at several international platforms including a featured interview by Technology Networks, USA, and won several awards. In 2020, Research Australia featured his work in their INSPIRE magazine as one of the top 15 innovations nationwide
Dr Syed is currently an Associate Lecturer at the University of Newcastle. His research work has focused on defining the molecular pathways involved in the pathogenesis of gynecological cancers, for which he has developed several models including cancer patient-derived-xenograft and -organoid models, and genetically modified mouse-models of cancer progression and metastasis. Using these cancer models along with uterus (the most regenerative organ) as the robust model organ for adult stem cell biology and tissue regeneration, his group employs a wide range of advanced sophisticated techniques including quantitative biology, cell-lineage tracing, high-throughput RNA-sequencing, proteomics, genomics, and advanced 3D-imaging to identify the basic principles involved in the regulation of growth and development and then extend these investigations to cancer and injury repair.
In his short career, Dr Syed has 21 refereed journal articles and book chapters, with an accompanying 123 citations and H-index of 7 and has published in top journals like Cell Stem Cell x1, Cell Reports x2, Development x1 and Developmental Biology x2. The innovative nature of his research is evidenced by being the first to identify novel endometrial stem cell markers and discover endometrial cancer-cell-of-origin. He has presented at over 15 national and international conferences and has been invited to write several chapters and review articles by renowned publishers including Nova Science Publishers, Inc., Springer, Developmental Biology, Molecular and Cellular Oncology. Also a member of the Hunter Cancer Research Alliance (HCRA) and Australian Society of Reproductive Biology, Syed is specialized in Cancer and Stem Cell Biology.Qualifications
- Doctor of Philosophy, University of Newcastle
Keywords
- Cancer Genetics
- Cancer biology
- Cancer stem cells
- Endometrial cancer
- Endometrial stem cells
- Endometriosis
- Gynecologic oncology
- Ovarian cancer
Languages
- English (Fluent)
Professional Experience
UON Appointment
Title | Organisation / Department |
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Cancer Institute NSW EC Fellow | University of Newcastle School of Biomedical Sciences and Pharmacy Australia |
Academic appointment
Dates | Title | Organisation / Department |
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1/1/2019 - 1/5/2021 | Associate Lecturer | The University of Newcastle - School of Biomedical Sciences and Pharmacy Faculty of Health Australia |
1/1/2012 - 1/7/2012 | Lecturer | G. B. Pant University of Agriculture and Technology, India India |
Teaching appointment
Dates | Title | Organisation / Department |
---|---|---|
1/1/2014 - 31/12/2018 | Casual Academic | School of Biomedical Sciences and Pharmacy, Faculty of Health and Medicine, University of Newcastle Australia |
Awards
Award
Year | Award |
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2020 |
Future Leaders Group Award HCRA Hunter Cancer Research Alliance |
2019 |
Highly Commended Rapid Reflection Oral Presentation Award HCRA Hunter Cancer Research Alliance |
2019 |
Stem Cells Australia Retreat Travel Grant The University of Melbourne |
2017 |
Society of Reproductive Biology Travel Grant Australian Society of Reproductive Biology |
2014 |
Beautiful Science Award School of Biomedical Sciences and Pharmacy, Faculty of Health and Medicine, University of Newcastle |
2012 |
Young Scientist Award Indian Veterinary Research Institute |
2012 |
University of Newcastle Postgraduate Research Scholarship Faculty of Health and Medicine, The University of Newcastle |
2012 |
University of Newcastle Research Scholarship Central (UNRSC) School of Biomedical Sciences and Pharmacy, Faculty of Health and Medicine, University of Newcastle |
Distinction
Year | Award |
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2012 |
CSIR Fellowship Council of Scientific and Industrial Research, Ministry of Science and Technology, Government of India |
2009 |
ICAR Junior Research Fellowship Indian Council of Agricultural Research, India |
2009 |
DBT Fellowship Department of Biotechnology, Ministry of Science and Technology, India |
Thesis Examinations
Year | Level | Discipline | Thesis |
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2019 | Honours | Health | Calcium imaging of a spinal cord amplifier circuit: in vitro analysis of calretinin neuron activity in the spinal dorsal horn |
2019 | Honours | Health | In vitro efficacy of ONC201 in diffuse intrinsic pontine glioma (DIPG) |
Teaching
Code | Course | Role | Duration |
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HUBS2209 |
Cancer Biology and Oncology School of Biomedical Sciences and Pharmacy, Faculty of Health and Medicine, University of Newcastle This course will cover the underlying molecular and cellular biology involved in carcinogenesis, tumor growth, and metastasis, and the implications of the basic biological findings on cancer prevention, diagnosis, and treatment. It involves integrated learning between the areas of Biochemistry, Cell Biology, Molecular Biology and Human Genetics. Students will also learn about the biologic hallmarks of cancer, genomic instability, dysregulated cancer cell metabolism, and the role of microenvironment, lifestyle factors, and immune system in cancer development and progression. The course is organised as a series of modules that include lectures and practical classes that provides students with a solid background in general cancer biology and prepares them for a career in laboratory-based research in areas of the biomedical sciences. |
Course Coordinator and Lecturer | 7/1/2021 - 7/1/2022 |
HUBS2107 |
Mammalian Growth and Development School of Biomedical Sciences and Pharmacy, Faculty of Health and Medicine, University of Newcastle This course will provide students with an overview of the biomolecular, cellular, genetic, physiological and anatomical aspects of reproductive growth and development, from the creation of the germ cells in the developing embryo to reproductive senescence in the ageing adult. The course will examine how sperm and eggs (gametes) are made; fertilisation achieved; and successful pregnancy initiated, maintained and stopped at birth. Developmental principles will be studied. The course will focus only on mammals with an emphasis on humans. Current research findings and techniques will be a key focus of this course. |
Course Coordinator | 7/1/2020 - 7/1/2021 |
HUBS2107 |
Mammalian Growth and Development School of Biomedical Sciences and Pharmacy, Faculty of Health and Medicine, University of Newcastle This course will provide students with an overview of the biomolecular, cellular, genetic, physiological and anatomical aspects of reproductive growth and development, from the creation of the germ cells in the developing embryo to reproductive senescence in the ageing adult. The course will examine how sperm and eggs (gametes) are made; fertilisation achieved; and successful pregnancy initiated, maintained and stopped at birth. Developmental principles will be studied. The course will focus only on mammals with an emphasis on humans. Current research findings and techniques will be a key focus of this course. |
Lecturer | 7/1/2019 - 7/1/2020 |
HUBS3412 |
Work Integrated Learning in Biomedical Science 1 School of Biomedical Sciences and Pharmacy, Faculty of Health and Medicine, University of Newcastle This course is a research or industry placement course, which provides an opportunity for a student to work as part of a research laboratory, or within industry. |
Examiner/Evaluator | 7/1/2019 - 7/1/2021 |
HUBS2505 |
Human Pathophysiology School of Biomedical Sciences and Pharmacy, Faculty of Health and Medicine, University of Newcastle This course provides an introduction to the concept of human disease and the pathophysiological mechanisms underlying the causes. Students will be provided with an introduction to pathophysiology of the immune, endocrine, circulatory, respiratory, gastrointestinal, musculoskeletal, renal and nervous systems. Associated pathologies of these systems will be introduced and explored. |
Lecturer | 22/2/2021 - 30/6/2021 |
HUBS3413 |
Work Integrated Learning in Biomedical Science 2 School of Biomedical Sciences and Pharmacy, Faculty of Health and Medicine, University of Newcastle This course is a research or industry placement course, which provides an opportunity for a student to work as part of a research laboratory, or within industry |
Examiner/Evaluator | 7/1/2019 - 7/1/2021 |
HUBS2206 |
Human Biochemistry and Cell Biology School of Biomedical Sciences and Pharmacy, Faculty of Health and Medicine, University of Newcastle This course will cover the underlying molecular and cellular biology involved in carcinogenesis, tumor growth, and metastasis, and the implications of the basic biological findings on cancer prevention, diagnosis, and treatment. It involves integrated learning between the areas of Biochemistry, Cell Biology, Molecular Biology and Human Genetics. Students will also learn about the biologic hallmarks of cancer, genomic instability, dysregulated cancer cell metabolism, and the role of microenvironment, lifestyle factors, and immune system in cancer development and progression. The course is organised as a series of modules that include lectures and practical classes that provides students with a solid background in general cancer biology and prepares them for a career in laboratory-based research in areas of the biomedical sciences. |
Course Coordinator | 22/2/2021 - 30/6/2021 |
VPB112 |
Veterinary Physiology and Biochemistry G. B. Pant University of Agriculture and Technology, India |
Lecturer | 1/1/2012 - 1/7/2012 |
Publications
For publications that are currently unpublished or in-press, details are shown in italics.
Chapter (6 outputs)
Year | Citation | Altmetrics | Link | |||||
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2022 |
Mohan H, Vandna, Soni S, Syed S, 'Targeting Reactive Oxygen Species (ROS) for Cancer Therapy', Handbook of Oxidative Stress in Cancer: Therapeutic Aspects: Volume 1 3181-3196 (2022) Reactive oxygen species (ROS) are the highly reactive molecules that play vital roles in cancer progression as well as in the regulation of important cellular pathways. Interestin... [more] Reactive oxygen species (ROS) are the highly reactive molecules that play vital roles in cancer progression as well as in the regulation of important cellular pathways. Interestingly, ROS also have beneficial roles such as it enhance the anti-tumorigenic signaling and increasing the cancer cell death by oxidative damage. ROS can be quenched by the antioxidant system, but these are not much effective at the time of the high level of ROS production, hence causing several pathological conditions such as tumor cell promotion and progression which affects the signaling pathways. It was observed that tumor cells generate a high level of ROS that results in increased metabolic rate, hypoxia, and mutation in the gene. Cancer cells also maintain a significant level of antioxidant enzymes that neutralize the effect of elevated ROS, indicating an intricate equilibrium of ROS is required for cancer cell survival and function. Further increase in ROS level may lead to programmed cell death (PCD). Elevated ROS levels generated by various metabolic pathways can work as Trojan horse for killing cancer cells. However, to precisely kill the cancer cells only signaling pathway that regulates diverse functions in cancer cells needs to be elucidated. This review focuses on ROS generation in tumor cells, their role in cancer biology, and the molecular mechanism of therapeutics based on altering the ROS level to treat cancer.
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2022 |
Mohan H, Vandna, Soni S, Syed S, 'Targeting Reactive Oxygen Species (ROS) for Cancer Therapy', Handbook of Oxidative Stress in Cancer: Therapeutic Aspects, Springer Nature Singapore 1-16 (2022)
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2021 |
Prasad M, Ghosh M, Suman, Patki HS, Kumar S, Brar B, et al., 'Imaging Techniques in Veterinary Disease Diagnosis', Advances in Animal Disease Diagnosis, CRC Press 103-145 (2021)
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2013 |
Minakshi P, Shafiq M, Alisha A, Ranjan K, Kumar P, Anshul B, et al., 'Animal forensics and applications', Biotechnology: Prospects and Applications 265-286 (2013) Forensic science is the application of a broad spectrum of sciences and technologies to investigate situations after getting the facts and to establish what occurred based on coll... [more] Forensic science is the application of a broad spectrum of sciences and technologies to investigate situations after getting the facts and to establish what occurred based on collected evidence. This is especially important in law enforcement where forensics is done in relation to criminal or civil law. In civil actions, forensics can help resolve a broad spectrum of legal issues through the identification, analysis, and evaluation of physical evidence. The field of forensic science covers document examination, DNA analysis using electronic or digital media, fingerprinting, autopsy techniques, forensic engineering, forensic anthropology, pathology, economics, accounting, biology, entomology, toxicology, and much more. In this chapter we have described different materials such as hair, blood, bone, teeth, saliva, nails, feathers, skin, leather, sperm, feces, and urine and different methods for extracting DNA from different sources. The applications of animal forensics can be broadly viewed in the following four categories such as animals can be the victim, can be the perpetrator, and can be the witness and wildlife forensics. Molecular animal forensics provides different genetic tools such as DNA sequencing, single nucleotide polymorphism (SNP), PCR-RFLP, and microsatellite analysis for species identification and for characterization or identification of a sample recovered from a crime scene or illegal wildlife traders and black markets involved in wildlife trade. The genetic identification can be done as species identification, identification of geographic origin, individual identification, etc. Mitochondrial and nuclear markers can be used for genetic identification of the species. Identification of geographic origin is done by phylogeography and population assignment methods. To summarize, various techniques of individual identification, sexing, and parentage can be used. These techniques involve the microsatellite genotyping, DNA nucleotide sequencing, SNP typing, RAPD, and AFLP.
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2013 |
Minakshi P, Ranjan K, Dahiya S, Pawan K, Rupinder, Shafiq, et al., 'Restriction enzyme analysis based differentiation of Indian isolates of Bluetongue Virus-1 isolated from Northern and Southern States of India', Microbial, Plant and Animal Research 219-223 (2013) Bluetongue (BT) is an economically important viral disease of sheep and goat causing high morbidity and mortality. In the present study, eight Bluetongue virus (BTV) isolates from... [more] Bluetongue (BT) is an economically important viral disease of sheep and goat causing high morbidity and mortality. In the present study, eight Bluetongue virus (BTV) isolates from Utter Pradesh (UP), four BTV isolates from Andhra Pradesh (AP) and one isolate each from Haryana, Rajasthan and Tamil Nadu (TN) states were adapted in BHK- 21 cell line. All the isolates were confirmed as bluetongue virus (BTV) based on amplification of 366bp amplicon with group specific ns1 gene based RT-PCR. All the isolates were then confirmed as BTV serotype-1 based on segment 2 (vp2 gene) specific RT-PCR amplifying a specific amplicon of 605bp size. The isolates were further confirmed as BTV-1 by nucleotide sequencing of partially amplified products of vp2 gene. The sequencing data thus generated was analysed to seek for any intraserotypic variations among these various Indian isolates of BTV-1 which originated from different states of India. For this purpose, restriction map for various restriction enzymes (RE) was constructed after carrying out in silico restriction enzyme analysis using Restriction Mapper V 3.0. software. In silico restriction enzyme analysis (REA) with restriction enzyme (RE) HindII, of two samples NRT35 (JQ037810) and NRT39 (JQ037809) from AP revealed a single RE site at nt1250. One isolate each from UP (MKD25 (JQ037807)), Rajasthan (AY559058) and Haryana (AY559060) also revealed a single RE site at nt1531, whereas remaining isolates revealed presence of two RE sites at nt1250 and nt1531. In case of TN (AY559061) and BT1 (JQ037811) isolate, REA with RE TaqI revealed one extra RE site at nt1244 in addition to the one at nt1513 in remaining isolates, where as one isolate each from UP (MKD22 (JQ037804)) and AP (NRT35 (JQ037810)) revealed one additional RE site at nt1285 and nt1685 respectively, with RE NdeII. A little genetic diversity was observed within BTV1 isolates of adjoining states such as Haryana, Rajasthan, Andhra Pradesh and Tamil Nadu due to point mutations. The presence of a common culicoides vector species C. oxystoma has been reported in all these states and could be the possible cause for spread of virus in these parts of India. © 2013 by Nova Science Publishers, Inc. All rights reserved. |
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2013 |
Shafiq M, Minakshi P, Ranjan AK, Ravinder P, Prasad G, 'Restriction enzyme analysis based differentiation of Indian and global isolates of BTV21', Microbial, Plant and Animal Research 91-96 (2013) One of the most important vector borne diseases of ruminants worldwide is causedby bluetongue (BT) virus, an orbivirus of the Reoviridae family. Bluetongue (BT) is aneconomically ... [more] One of the most important vector borne diseases of ruminants worldwide is causedby bluetongue (BT) virus, an orbivirus of the Reoviridae family. Bluetongue (BT) is aneconomically important viral disease of ruminant species especially sheep and goatcausing high morbidity and mortality.The BT virus has segmented genome and is thus prone to frequent mutations andgene reassortment following the co-infection of two or more viruses in the host cell,leading to emergence of new genetic variants. For the present study, vp2 gene of Indianisolate KMNO-7 of BTV21, adapted in BHK-21 cell line, was sequenced full lengthutilising piecemeal approach after designing 7 primer pairs for the same and in silicorestriction enzyme analysis (REA) was done.The Restriction enzyme profile of the vp2 gene of the Indian isolate was thencompared with that of the other global isolates of BTV21 namely BTV21/ japan/TO2-1(AB686218.1), BTV21/japan/ ON89-1 (AB686215.1) and BTV21/SA-ref (AJ585142.1).Such comparison revealed certain restriction sites which were unique to the isolates andthus could help in differentiating Indian isolate from the global isolates. It was found thatthe restriction site for the enzyme EcoRV was present exclusively in the Indian isolateKMNO-7 while other isolates lacked the site for the enzyme. Similarly restriction site forthe enzyme Xbal1 was present exclusively in the BTV21/japan/ON89-1 isolate and not inthe other isolates. BTV21/SA-ref (AJ585142.1) lacked the restriction sites for theenzymes Sal1, Sca1 and BamH1 whereas other isolates possessed the restriction sites forthese enzymes. The restriction site for the enzyme Hind111 was absent inBTV21/japan/ON89-1isolate but was present in the remaining three isolates. Although there are various nucleotide sequence based methods for the differentiation of variousstrains of bluetongue virus, however, REA can serve as a quick method of differentiatingglobal isolates of a particular bluetongue virus serotype which can be furthercharacterised after deducing the nucleotide sequence of the vp2 gene. © 2013 by Nova Science Publishers, Inc. All rights reserved. |
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Journal article (15 outputs)
Year | Citation | Altmetrics | Link | ||||||||
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2022 |
Jamaluddin MFB, Ko Y-A, Ghosh A, Syed SM, Ius Y, O'Sullivan R, et al., 'Proteomic and functional characterization of intra-tumor heterogeneity in human endometrial cancer', CELL REPORTS MEDICINE, 3 (2022) [C1]
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2020 |
Ali A, Syed SM, Tanwar PS, 'Protocol for
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2020 |
Ali A, Syed SM, Jamaluddin MFB, Colino-Sanguino Y, Gallego-Ortega D, Tanwar PS, 'Cell Lineage Tracing Identifies Hormone-Regulated and Wnt-Responsive Vaginal Epithelial Stem Cells', Cell Reports, 30 1463-1477.e7 (2020) [C1] The intact vaginal epithelium is essential for women's reproductive health and provides protection against HIV and sexually transmitted infections. How this epithelium mainta... [more] The intact vaginal epithelium is essential for women's reproductive health and provides protection against HIV and sexually transmitted infections. How this epithelium maintains itself remains poorly understood. Here, we used single-cell RNA sequencing (RNA-seq) to define the diverse cell populations in the vaginal epithelium. We show that vaginal epithelial cell proliferation is limited to the basal compartment without any obvious label-retaining cells. Furthermore, we developed vaginal organoids and show that the basal cells have increased organoid forming efficiency. Importantly, Axin2 marks a self-renewing subpopulation of basal cells that gives rise to differentiated cells over time. These cells are ovariectomy-resistant stem cells as they proliferate even in the absence of hormones. Upon hormone supplementation, these cells expand and reconstitute the entire vaginal epithelium. Wnt/ß-catenin is essential for the proliferation and differentiation of vaginal stem cells. Together, these data define heterogeneity in vaginal epithelium and identify vaginal epithelial stem cells.
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Nova | |||||||||
2020 |
Minakshi P, Mohan H, Manjeet, Ravina, Brar B, Shafiq M, Pundir CS, 'Organic Polymer and Metal Nano-particle Based Composites for Improvement of the Analytical Performance of Electrochemical Biosensor', CURRENT TOPICS IN MEDICINAL CHEMISTRY, 20 1029-1041 (2020) [C1]
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2020 |
Syed SM, Tanwar PS, 'Axin2(+) endometrial stem cells: the source of endometrial regeneration and cancer', MOLECULAR & CELLULAR ONCOLOGY, 7 (2020) [C1]
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Nova | |||||||||
2020 |
Syed SM, Kumar M, Ghosh A, Tomasetig F, Ali A, Whan RM, et al., 'Endometrial Axin2 Cells Drive Epithelial Homeostasis, Regeneration, and Cancer following Oncogenic Transformation', Cell Stem Cell, 26 64-80.e13 (2020) [C1]
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2020 |
Ghosh A, Syed SM, Kumar M, Carpenter TJ, Teixeira JM, Houairia N, et al., 'In Vivo Cell Fate Tracing Provides No Evidence for Mesenchymal to Epithelial Transition in Adult Fallopian Tube and Uterus', CELL REPORTS, 31 (2020) [C1]
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2019 |
Al-Juboori AAA, Ghosh A, Bin Jamaluddin MF, Kumar M, Sahoo SS, Syed SM, et al., 'Proteomic Analysis of Stromal and Epithelial Cell Communications in Human Endometrial Cancer Using a Unique 3D Co-Culture Model', PROTEOMICS, 19 (2019) [C1]
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2017 |
Goad J, Ko YA, Kumar M, Syed SM, Tanwar PS, 'Differential Wnt signaling activity limits epithelial gland development to the anti-mesometrial side of the mouse uterus', Developmental Biology, 423 138-151 (2017) [C1]
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2017 |
Ghosh A, Syed SM, Tanwar PS, 'In vivo genetic cell lineage tracing reveals that oviductal secretory cells self-renew and give rise to ciliated cells', Development (Cambridge), 144 3031-3041 (2017) [C1] The epithelial lining of the fallopian tube is vital for fertility, providing nutrition to gametes and facilitating their transport. It is composed of two major cell types: secret... [more] The epithelial lining of the fallopian tube is vital for fertility, providing nutrition to gametes and facilitating their transport. It is composed of two major cell types: secretory cells and ciliated cells. Interestingly, human ovarian cancer precursor lesions primarily consist of secretory cells. It is unclear why secretory cells are the dominant cell type in these lesions. Additionally, the underlying mechanisms governing fallopian tube epithelial homoeostasis are unknown. In the present study, we showed that across the different developmental stages of mouse oviduct, secretory cells are the most frequently dividing cells of the oviductal epithelium. In vivo genetic cell lineage tracing showed that secretory cells not only self-renew, but also give rise to ciliated cells. Analysis of a Wnt reporter mouse model and various Wnt target genes showed that the Wnt signaling pathway is involved in oviductal epithelial homoeostasis. By developing two triple-transgenic mouse models, we showed that Wnt/ ß-catenin signaling is essential for self-renewal as well as the differentiation of secretory cells. In summary, our results provide mechanistic insight into oviductal epithelial homoeostasis.
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2016 |
Kumar M, Syed SM, Taketo MM, Tanwar PS, 'Epithelial Wnt/ßcatenin signalling is essential for epididymal coiling', Developmental Biology, 412 234-249 (2016) [C1] Organ shape and size are important determinants of their physiological functions. Epithelial tubes are anlagen of many complex organs. How these tubes acquire their complex shape ... [more] Organ shape and size are important determinants of their physiological functions. Epithelial tubes are anlagen of many complex organs. How these tubes acquire their complex shape and size is a fundamental question in biology. In male mice, the Wolffian duct (WD; postnatally known as epididymis) undergoes an astonishing transformation, where a straight tube only a few millimetres long elongates to over 1000 times its original length and fits into a very small space, due to extensive coiling of epithelium, to perform the highly specialized function of sperm maturation. Defective coiling disrupts sperm maturation and leads to male infertility. Recent work has shown that epithelial cell proliferation is a major driver of WD coiling. Still, very little is known about the molecular signals involved in this process. Testicular androgens are known regulators of WD development. However, epithelial androgen receptor signalling is dispensable for WD coiling. In this study, we have shown that Wnt signalling is highly active in the entire WD epithelium during its coiling, and is limited to only a few segments of the epididymis in later life. Pharmacological and genetic suppression of Wnt signalling inhibited WD coiling by decreasing cell proliferation and promoting apoptosis. Comparative gene expression analysis identified Fibroblast growth factor 7 (Fgf7) as a prime Wnt target gene involved in WD coiling and in vitro treatment with Fgf7 protein increased coiling of WDs. In summary, our work has established that epithelial canonical Wnt signalling is a critical regulator of WD coiling and its precise regulation is essential for WD/epididymal differentiation.
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2014 |
Kumar R, Gupta M, Shafiq S, Balhara AK, Em S, Ghosh M, Singh I, 'Sequencing-based polymorphism in leptin receptor gene and lack of association with postpartum anestrus in Murrah buffaloes', Turkish Journal of Veterinary and Animal Sciences, 38 670-674 (2014) In order to detect polymorphism in exon 20 of the leptin receptor (LEPR) gene and its possible association with postpartum anestrus (PPA), peripheral blood samples were obtained f... [more] In order to detect polymorphism in exon 20 of the leptin receptor (LEPR) gene and its possible association with postpartum anestrus (PPA), peripheral blood samples were obtained from 40 Murrah buffaloes including 20 PPA (>120 days after parturition) and 20 normal cyclic (<60 days after parturition) buffaloes having similar postpartum estrous conditions over previous consecutive gestations. Genomic DNA was isolated, and PCR was standardized to amplify partial exon 20 of the LEPR gene of 413 bp. Amplified fragments of the gene were sequenced and sequence variation was detected by assessing multiple alignments. The LEPR gene showed polymorphism at A231G, C247A, and G347A. Chi-square test of these 3 polymorphism sites between the 2 groups did not reveal any significant association of polymorphism with PPA. Hence, polymorphism at exon 20 of the LEPR gene was found in Murrah buffaloes, but the role of these polymorphisms in PPA could not be established.
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2013 |
Shafiq M, Minakshi P, Bhateja A, Ranjan K, Prasad G, 'Evidence of genetic reassortment between Indian isolate of bluetongue virus serotype 21 (BTV-21) and bluetongue virus serotype 16 (BTV-16)', Virus Research, 173 336-343 (2013) The genome of bluetongue virus (BTV) consists of 10 segments. Of these seg-2 encoded VP2 is the major serotype determining protein, and seg-6 encoded VP5 protein enhances the prot... [more] The genome of bluetongue virus (BTV) consists of 10 segments. Of these seg-2 encoded VP2 is the major serotype determining protein, and seg-6 encoded VP5 protein enhances the protective neutralizing activity of VP2 protein inducing higher serotype specific antibody titer than the VP2 alone. Out of the twenty-six BTV serotypes found worldwide, 22 were reported from different states of India. These include serotype 21 which was recently isolated from Andhra Pradesh, and was involved in a severe outbreak of bluetongue in Indian native sheep. BTV21 (KMNO-7) and BTV16 were circulating at the same time. This co-circulation, along with the fact that the virus genome is segmented, provides an opportunity for these two isolates of different serotypes to simultaneously infect the same animal, and even the same cell or a same vector with the potential for generation of reassortant viruses. This study was carried out to provide some insights into the outbreak. We carried out full length sequencing of genome seg-2 and seg-6 of Indian isolates VJW64 (BTV16) and KMNO-7 (BTV21). Detailed phylogenetic analysis revealed that genome seg-6 of Indian isolate KMNO-7 (BTV21) clusters with isolates of BTV16 showing maximum nucleotide similarity of 97.6% with TUR/2000/02 isolate of BTV16, which is much more than it shows with any isolate of BTV21. KMNO-7 (BTV21) significantly diverged from original strain of BTV21, and is a reassortant strain having acquired seg-6 from an isolate of BTV16. This study provides some useful insights into the epidemiology of the bluetongue disease, and undermines serotyping on genome seg-6 basis. © 2013 Elsevier B.V.
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Show 12 more journal articles |
Conference (10 outputs)
Year | Citation | Altmetrics | Link | ||
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2019 |
Syed S, Lombard J, Mandaliya H, Nahar P, Ius Y, O'Sullivan R, et al., 'Axin2 Marks the Cell of Origin for Endometrial Cancer', ASIA-PACIFIC JOURNAL OF CLINICAL ONCOLOGY (2019)
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2019 |
Jamaluddin M, Ko Y-A, Syed S, Ius Y, O'Sullivan R, Netherton J, et al., 'Understanding the basis of intra-tumour heterogeneity in human endometrial cancer', Newcastle City Hall, Newcastle, NSW, Australia (2019)
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2017 |
Syed SM, Tanwar PS, 'Ignore no more: Role of uterine aging in fertility', Perth (2017)
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2015 |
Kumar M, Goad J, Syed S, Tanwar PS, 'Twists and Turns: Balanced Wnt signalling is essential for epididymal coiling', Adelaide, SA, Australia (2015) [E3]
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2015 |
Goad J, Kumar M, Syed S, Tanwar PS, 'Gone with the Wnt: Unopposed oestrogen leads to endometrial cancer by regulating Wnt signaling', Adelaide, SA, Australia (2015) [E3]
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2015 |
Goad J, Syed S, Tanwar PS, 'Synergistic effect of unopposed oestrogen and overactive Wnt signalling in endometrial cancer', Newcastle, NSW, Australia (2015) [E3]
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2015 |
Syed S, Goad J, Sahoo S, Cardona J, Kumar J, Tanwar P, 'Endometrial hyperplasia and cancer: Side effects of miscommunication', Adelaide, SA, Australia (2015) [E3]
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2015 |
Syed S, Goad J, Sahoo S, Cardona J, Tanwar PS, 'Influence of bad neighborhood: Stromal contribution to endometrial cancer development', Newcastle, NSW, Australia (2015) [E3]
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Show 7 more conferences |
Grants and Funding
Summary
Number of grants | 9 |
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Total funding | $1,500,845 |
Click on a grant title below to expand the full details for that specific grant.
20241 grants / $634,721
Dietary and metabolic control of Axin2+ stem cells in endometrial physiology and cancer$634,721
Funding body: NHMRC (National Health & Medical Research Council)
Funding body | NHMRC (National Health & Medical Research Council) |
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Project Team | Doctor Shafiq Syed, Doctor Shafiq Syed |
Scheme | Investigator Grant |
Role | Lead |
Funding Start | 2024 |
Funding Finish | 2028 |
GNo | G2300237 |
Type Of Funding | C1100 - Aust Competitive - NHMRC |
Category | 1100 |
UON | Y |
20231 grants / $48,545
A fatty link between stem cells and endometrial cancer: Understanding the role of microenvironment and Axin2+ stem cell crosstalk in obesity associated endometrial cancer, metastasis, and drug resista$48,545
Funding body: Cure Cancer Australia Foundation
Funding body | Cure Cancer Australia Foundation |
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Project Team | Doctor Shafiq Syed |
Scheme | Research Grant |
Role | Lead |
Funding Start | 2023 |
Funding Finish | 2024 |
GNo | G2201269 |
Type Of Funding | C1700 - Aust Competitive - Other |
Category | 1700 |
UON | Y |
20222 grants / $100,000
A fatty link between stem cells and endometrial cancer: Understanding the role of Axin2+ stem cells in endometrial regeneration, cancer, and metastasis$50,000
Funding body: Cancer Australia
Funding body | Cancer Australia |
---|---|
Project Team | Doctor Shafiq Syed, Doctor Arnab Ghosh, Doctor Ken Jaaback |
Scheme | Priority-driven Collaborative Cancer Research Scheme |
Role | Lead |
Funding Start | 2022 |
Funding Finish | 2022 |
GNo | G2100584 |
Type Of Funding | C1500 - Aust Competitive - Commonwealth Other |
Category | 1500 |
UON | Y |
A fatty link between stem cells and endometrial cancer: Understanding the role of Axin2+ stem cells in endometrial regeneration, cancer, and metastasis$50,000
Funding body: Cure Cancer Australia Foundation
Funding body | Cure Cancer Australia Foundation |
---|---|
Project Team | Doctor Shafiq Syed |
Scheme | Research Grant |
Role | Lead |
Funding Start | 2022 |
Funding Finish | 2022 |
GNo | G2200183 |
Type Of Funding | C1700 - Aust Competitive - Other |
Category | 1700 |
UON | Y |
20212 grants / $614,721
From Benchside to Bedside: Development of novel targeted strategies for the prevention and conservative management of obesity-driven endometrial cancer$599,721
Funding body: Cancer Institute NSW
Funding body | Cancer Institute NSW |
---|---|
Project Team | Doctor Shafiq Syed, Doctor Shafiq Syed, Professor Ajayan Vinu |
Scheme | Early Career Fellowship |
Role | Lead |
Funding Start | 2021 |
Funding Finish | 2023 |
GNo | G2000902 |
Type Of Funding | C2300 – Aust StateTerritoryLocal – Own Purpose |
Category | 2300 |
UON | Y |
Development of a diagnostic and prognostic blood-based test for endometriosis$15,000
Funding body: College of Health, Medicine and Wellbeing, UoN
Funding body | College of Health, Medicine and Wellbeing, UoN |
---|---|
Project Team | Dr Shafiq Syed, Dr Arnab Ghosh, Dr Muhammad Jamaluddin, Dr Manish Kumar, Professor Pradeep Tanwar, Dr Pravin Nahar, Dr Ken Jaaback |
Scheme | 2021 Strategic Research Pilot Grants |
Role | Lead |
Funding Start | 2021 |
Funding Finish | 2021 |
GNo | |
Type Of Funding | Other Public Sector - Local |
Category | 2OPL |
UON | N |
20202 grants / $6,858
HCRA Biomarkers and Targeted Therapies Flagship Program-2020 Infrastructure Support$4,998
Funding body: Hunter Cancer Research Alliance (HCRA)
Funding body | Hunter Cancer Research Alliance (HCRA) |
---|---|
Scheme | Infrastructure Funding |
Role | Lead |
Funding Start | 2020 |
Funding Finish | 2021 |
GNo | |
Type Of Funding | C3120 - Aust Philanthropy |
Category | 3120 |
UON | N |
Axin2 marks the cell of origin for endometrial cancer$1,860
Funding body: Hunter Cancer Research Alliance (HCRA)
Funding body | Hunter Cancer Research Alliance (HCRA) |
---|---|
Project Team | Dr Shafiq Syed, Associate Professor Pradeep Tanwar |
Scheme | Statistical support |
Role | Lead |
Funding Start | 2020 |
Funding Finish | 2020 |
GNo | |
Type Of Funding | C3120 - Aust Philanthropy |
Category | 3120 |
UON | N |
20191 grants / $96,000
Endometrial Cancer Research Project$96,000
Funding body: Hunter Medical Research Institute
Funding body | Hunter Medical Research Institute |
---|---|
Project Team | Professor Pradeep Tanwar, Doctor Shafiq Syed, Doctor Muhammad Fairuz Jamaluddin |
Scheme | Project Grant |
Role | Investigator |
Funding Start | 2019 |
Funding Finish | 2019 |
GNo | G1900346 |
Type Of Funding | C3300 – Aust Philanthropy |
Category | 3300 |
UON | Y |
Research Supervision
Number of supervisions
Current Supervision
Commenced | Level of Study | Research Title | Program | Supervisor Type |
---|---|---|---|---|
2023 | PhD | Role Of Stem/Progenitor Cells In Endometrial Biology And Carcinogenesis | PhD (Medical Biochemistry), College of Health, Medicine and Wellbeing, The University of Newcastle | Co-Supervisor |
2023 | PhD | Patient-derived Organoids for Gynaecological Diseases | PhD (Medical Biochemistry), College of Health, Medicine and Wellbeing, The University of Newcastle | Co-Supervisor |
2023 | PhD | Patient Derive Organoid As A Model For Drug Screening In Women Suffering From Endometrial Cancer | PhD (Medical Biochemistry), College of Health, Medicine and Wellbeing, The University of Newcastle | Co-Supervisor |
2023 | PhD | Using Preclinical Models to Improve Outcomes for Gynaecological Diseases – Endometrial Organoid to Study Drug Resistance in Gynaecological Diseases | PhD (Medical Biochemistry), College of Health, Medicine and Wellbeing, The University of Newcastle | Co-Supervisor |
2022 | PhD | Comparative Proteomic Analysis of the Organoids from Endometriosis Patients with the Organoids from Normal Endometrium and Endometrial Cancer | PhD (Medical Genetics), College of Health, Medicine and Wellbeing, The University of Newcastle | Co-Supervisor |
2022 | PhD | Using OMICS based Approaches for Personalizing Treatments for Gynecological Cancer Patients | PhD (Medical Biochemistry), College of Health, Medicine and Wellbeing, The University of Newcastle | Co-Supervisor |
2021 | Honours | Understand the basis of menses: how uterus performs scarless repair | Health, School of Biomedical Sciences and Pharmacy, Faculty of Health and Medicine, University of Newcastle | Principal Supervisor |
2020 | Honours | Endometrial stem cells and their role in endometriosis, infertility and cancer | Biochemistry & Cell Biology, School of Biomedical Sciences and Pharmacy, Faculty of Health and Medicine, University of Newcastle | Co-Supervisor |
Past Supervision
Year | Level of Study | Research Title | Program | Supervisor Type |
---|---|---|---|---|
2019 | Unknown | Understand the basis of menses: how uterus performs scarless repair | Biochemistry & Cell Biology, School of Biomedical Sciences and Pharmacy, Faculty of Health and Medicine, University of Newcastle | Co-Supervisor |
2019 | Honours | The role of endometrial stem cells in the origin of ovarian cancer | Biochemistry & Cell Biology, School of Biomedical Sciences and Pharmacy, Faculty of Health and Medicine, University of Newcastle | Co-Supervisor |
2019 | Unknown | Role of Stem cells in ovarian cancer development | Biochemistry & Cell Biology, School of Biomedical Sciences and Pharmacy, Faculty of Health and Medicine, University of Newcastle | Co-Supervisor |
News
News • 15 Dec 2023
$10.3m NHMRC Investigator grants help search for health solutions
Six University of Newcastle researchers have been awarded more than $10.3m in National Health and Medical Research Council (NHMRC) Investigator grants* to tackle health problems such as pre-term labour, schizophrenia, endometrial and lung cancers, as well as asthma.
News • 3 Feb 2023
Early-career researchers boosted in mission to beat cancer
On the eve of World Cancer Day on 4 February, four University of Newcastle early career cancer researchers have been awarded $360,000 in grants to support their work investigating blood, gynaecological and colorectal cancers.
Dr Shafiq Syed
Position
Cancer Institute NSW EC Fellow
Associate Prof Pradeep Tanwar Group
School of Biomedical Sciences and Pharmacy
College of Health, Medicine and Wellbeing
Contact Details
shafiq.syed@newcastle.edu.au | |
Phone | (02) 4921 7280 |
Links |
Research Networks Research Networks |
Office
Room | LS-348 |
---|---|
Building | Life Sciences Building |
Location | Callaghan University Drive Callaghan, NSW 2308 Australia |