Dr Rick Thorne

HCRA Resource Manager

School of Biomedical Sciences and Pharmacy (Medical Biochemistry)

Career Summary

Biography

Dr. Rick Thorne (PhD 1999) is a postdoctoral researcher sponsored by the HMRI Cancer Research Program and contracted lecturer in the School of Biomedical Sciences & Pharmacy at the University of Newcastle.

His major interest involves different families of cell adhesion molecules and how these engage signalling pathways, particularly how these contribute to the development and progression of cancer. These interests overlap with CD36, another signalling molecule with disease functions outside cancer, particularly the metabolic syndrome and related disorders such as diabetes.

Dr. Thorne has over ten years postdoctoral experience beginning with the award of a two year competitive postdoctoral fellowship Brawn Postdoctoral Research Fellow undertaken at the UoN under Prof. Gordon Burns. During that period he was awarded two competitive overseas training schemes through the UICC, an International Cancer Technology Transfer Fellowships undertaken at the ICRF in London, UK (2000) and a Cancer Research Training Course held in Toronto, Canada (2001). Pursing further training he then relocated the UK to take up a three year post as a Postdoctoral Fellow in Training (2002-2004) at the 5* Institute of Cancer Research (ICR, London).

This appointment also led to a number of quality publications and first-hand experience as a PhD supervisor and supervisor of graduate student projects for Imperial College (honours equivalent). Following the ICR appointment he returned to UoN under an ARC Australian Postdoctoral Fellowship awarded from 2004-2007 in connection with an ARC Discovery Project. Subsequent to this award he received a three year Cancer Institute NSW fellowship for the period 2008-2010. After a number of years acting as laboratory manager for the Cancer Research Unit, Dr. Thorne inherited the facilities’ of Prof. Burns and now runs this laboratory on an independent basis.

Over the last five years Dr. Thorne has contributed 27 original research articles and one review article. For ten of these publications he is the first, equal contributory or senior author. Within this body of work there is a strong indication of productive collaborations with local, national and international colleagues.

Research Expertise
I have been working in laboratory-based science for nearly twenty years with direct hands on experience in vast array of techniques. I have a strong interest in the technical aspects of science, and have been instrumental in obtaining key equipment for my own research work and for the greater benefit of my research colleagues.

A summary of my technical skills and experience is as follows:

Molecular Biology: All DNA manipulations associated with the construction of expression vectors including epitope tagged molecules, fusion proteins, mutants and chimeras, recombinant adenoviruses. DNA and RNA preparation and analysis by southern and northern blotting, PCR, screening assays for cDNA libraries, yeast-2-hybrid assays etc.

Protein analysis and biochemistry: SDS-PAGE, 2D gels, immunoprecipitation, GST-pulldown, immunoblotting, use of radioisotopes, kinase assays, metabolic labeling studies, N-glycan analysis, chromatographic and related biochemical methods, flow cytometry, ELISA assays, protein and enzyme assays.

Imaging: I have developed and manage a microscope facility consisting of a trio of three Zeiss microscopes freely available to all subject to appropriate training.

All utilise a common software package (Axiovision) that helps in training of users. I have a great deal of experience in immunofluorescence and confocal microscopic imaging. The Axioplan 2 is a standard upright miscroscope equipped with colour and monochrome cameras, Apotome imaging device, Colibri 2 illumination system. This is powerful system fitted with a large number of epifluorescence filter sets and high performance objectives. This system is adaptable to almost any specimen on a slide. The Axiovert 200 is a manual inverted fluorescence microscope fitted with a high resolution monochrome camera. Suitable for general work using cultured cells. The Cell Observer System is for live cell imaging and more complex scanning tasks. It consists of an automated inverted microscope with scanning stage and an integrated incubator system. Cell culture and in vitro assays: derivation and maintenance of cell lines, cryopreservation, mycoplasma screening, cell transfection methods, cloning and cell sorting, production and characterization of monoclonal antibodies, cell adhesion, migration and chemotaxis assays, drug toxicity, apoptosis, phagocytosis and cell-mediated immunity assays. In vivo models: I have developed experimental models for cancer metastasis and immunoscintography in human xenograft models of melanoma, and experimental chemotherapy protocols for an rodent model of acquired drug-resistance in mammary cancer.

Teaching Expertise
Research Higher Degree Completions Mr. George Tzircotis (PhD 2005, University of London, UK) co-supervisor Ms. Kristy Shipman (PhD 2009, University of Newcastle, Australia) co-supervisor Mr. Mohammad Alkhatatbeh (thesis accepted December, 2012) primary supervisor Current Research Higher Degree Students As primary supervisor Ms. Camila Oliveira (enrolment confirmed, thesis submission due November 2012) Mr. Alireza Arjmand (enrolment confirmed, thesis submission due February 2012) Ms. Elham Sadeqzadeh (enrolment confirmed, thesis submission due February 2012) Mr. Abdulrzag Ahmed (enrolment confirmed, thesis submission due February 2014) As co-supervisor Ms. Jiao Li (other supervisors A/Prof. Derek Laver and A/Prof. Dirk Van Helden)

Additionally I have sat on a number of RHD confirmation panels for students from both the School of Biological Sciences and the School of Medicine and Public Health. Honours (and equivalent) supervision Ms. Ka Man Emily Chu (Final Year Project, Imperial College London, 2003) Ms. Dale Anne Boyce (Honours, Bachelor of Biomedical Sciences, University of Newcastle, 2005) Mr. Andrew James William Weir (Honours, Bachelor of Biomedical Sciences, University of Newcastle, 2007) Mr. Simon Bone (Honours, Bachelor of Biomedical Sciences, University of Newcastle, 2007) Mr. Steven Alley (Honours, Bachelor of Biomedical Sciences, University of Newcastle, 2009) Mr. Tim Kelso (Honours, Bachelor of Biomedical Sciences, University of Newcastle, 2010) Ms. Anna Timofeeva (Final Year Project, Karolinska Institute, Sweden, 2011) Undergraduate Teaching experience Contracted 0.5 lecturing appointment for undergraduate teaching in the School of Biomedical Sciences Bachelor’s program in 2001 and 2012 where I lectured major components of HUBS 2206 (Human Biochemistry and Cell Biology), HUBS 2209 (Human Molecular Science) and supervised associated Laboratory Practical Classes Course coordinator of HUBS 3409 (Project in Biomedical Sciences) in 2011 and 2012

Administrative Expertise
Membership of the Steering committee of the HMRI Cancer Research Program (2005-current) Organising committee member and session chairs for the HMRI Translational Cancer Conferences held in 2006 and 2008 Convener of the HMRI CRP seminar program from 2005-2009 HMRI Grant Review Panel Member 2009 Ad hoc representation on various committees at the School and Faculty level acting on behalf of Cancer Researchers and/or the discipline of Medical Biochemistry Reviewer of project grant applications in Research Infrastructure Block Grant (RIBG) Scheme (2006) Peer reviewer for both ARC Discovery and NHMRC Project grants since 2007 Peer reviewer for a number of scientific journals including Biochemical Journal, Journal of Lipid Research, Experimental Cell Research, Immunology and Cell Biology, Journal of Signal Transduction, Stem Cell Reviews & Reports and the British Journal of Cancer Member of the NHMRC GRPs in 2011 and 2012 assessing grants for Cancer Biology & Oncology


Qualifications

  • PhD, University of Newcastle
  • Bachelor of Science, University of Newcastle
  • Bachelor of Science (Honours), University of Newcastle

Keywords

  • apoptosis
  • cell adhesion
  • cell signaling

Fields of Research

CodeDescriptionPercentage
110199Medical Biochemistry and Metabolomics not elsewhere classified30
110399Clinical Sciences not elsewhere classified30
060199Biochemistry and Cell Biology not elsewhere classified40

Professional Experience

UON Appointment

DatesTitleOrganisation / Department
1/01/2012 - 31/12/2012Postdoctoral Research FellowUniversity of Newcastle
School of Biomedical Sciences and Pharmacy
Australia
1/01/2001 - 2/10/2001Brawn FellowUniversity of Newcastle
Medicine and Health Sciences
Australia

Academic appointment

DatesTitleOrganisation / Department
1/01/2010 - 1/12/2012Postdoctoral Fellow and Contracted LecturerUniversity of Newcastle
School of Biomedical Sciences and Pharmacy
Australia
1/01/2009 - Membership - American Association of Cancer ResearchAmerican Association of Cancer Research
United States
1/01/2008 - 1/12/2010CI NSW Career Development Postdoctoral FellowUniversity of Newcastle
School of Biomedical Sciences and Pharmacy
Australia
1/06/2004 - 1/06/2007ARC Postdoctoral Research FellowUniversity of Newcastle
School of Biomedical Sciences and Pharmacy
Australia
1/05/2001 - 1/05/2004Postdoctoral Fellow in TrainingInsititute of Cancer Research, London
Breakthrough Breast Cancer Research Centre
United States
1/01/2000 - 1/04/2001Fellowship - Gladys M Brawn Memorial FellowshipUniversity of Newcastle
1/01/2000 - 1/05/2001Gladys M. Brawn Postdoctoral Research FellowUniversity of Newcastle
School of Biomedical Sciences and Pharmacy
Australia

Membership

DatesTitleOrganisation / Department
Lifetime Fellow of the UICC OrganisationThe Union for International Cancer Control (UICC)
Australia

Awards

Research Award

YearAward
2007
Unknown

Invitations

Participant

YearTitle / Rationale
2006Dr. A. Obaidat, Department of Pharmacy
Organisation: Jordan University of Science and Technology, King Abdullah II Hospital
Edit

Publications

For publications that are currently unpublished or in-press, details are shown in italics.


Journal article (67 outputs)

YearCitationAltmetricsLink
2015Zouikr I, Ahmed AF, Horvat JC, Beagley KW, Clifton VL, Ray A, et al., 'Programming of formalin-induced nociception by neonatal LPS exposure: Maintenance by peripheral and central neuroimmune activity', Brain, Behavior, and Immunity, 44 235-246 (2015)

The immune and nociceptive systems are shaped during the neonatal period where they undergo fine-tuning and maturation. Painful experiences during this sensitive period of develop... [more]

The immune and nociceptive systems are shaped during the neonatal period where they undergo fine-tuning and maturation. Painful experiences during this sensitive period of development are known to produce long-lasting effects on the immune and nociceptive responses. It is less clear, however, whether inflammatory pain responses are primed by neonatal exposure to mild immunological stimuli, such as with lipopolysaccharide (LPS). Here, we examine the impact of neonatal LPS exposure on inflammatory pain responses, peripheral and hippocampal interleukin-1ß (IL-1ß), as well as mast cell number and degranulation in preadolescent and adult rats. Wistar rats were injected with LPS (0.05 mg/kg IP, Salmonella enteritidis) or saline on postnatal days (PNDs) 3 and 5 and later subjected to the formalin test at PNDs 22 and 80-97. At both time-points, and one-hour after formalin injection, blood and hippocampus were collected for measuring circulating and central IL-1ß levels using ELISA and Western blot, respectively. Paw tissue was also isolated to assess mast cell number and degree of degranulation using Toluidine Blue staining. Behavioural analyses indicate that at PND 22, LPS-challenged rats displayed enhanced flinching (p<.01) and licking (p<.01) in response to formalin injection. At PNDs 80-97, LPS-challenged rats exhibited increased flinching (p<.05), an effect observed in males only. Furthermore, neonatal LPS exposure enhanced circulating IL-1ß and mast cell degranulation in preadolescent but not adult rats following formalin injection. Hippocampal IL-1ß levels were increased in LPS-treated adult but not preadolescent rats in response to formalin injection. These data suggest neonatal LPS exposure produces developmentally regulated changes in formalin-induced behavioural responses, peripheral and central IL-1ß levels, as well as mast cell degranulation following noxious stimulation later in life. These findings highlight the importance of immune activation during the neonatal period in shaping immune response and pain sensitivity later in life. This is of clinical relevance given the high prevalence of bacterial infection during the neonatal period, particularly in the vulnerable population of preterm infants admitted to neonatal intensive care units.

DOI10.1016/j.bbi.2014.10.014
CitationsScopus - 1
Co-authorsPhilip Hansbro, Jay Horvat, Deborah Hodgson
2015Pundavela J, Roselli S, Faulkner S, Attia J, Scott RJ, Thorne RF, et al., 'Nerve fibers infiltrate the tumor microenvironment and are associated with nerve growth factor production and lymph node invasion in breast cancer', Molecular Oncology, (2015)

Infiltration of the tumor microenvironment by nerve fibers is an understudied aspect of breast carcinogenesis. In this study, the presence of nerve fibers was investigated in a co... [more]

Infiltration of the tumor microenvironment by nerve fibers is an understudied aspect of breast carcinogenesis. In this study, the presence of nerve fibers was investigated in a cohort of 369 primary breast cancers (ductal carcinomas in situ, invasive ductal and lobular carcinomas) by immunohistochemistry for the neuronal marker PGP9.5. Isolated nerve fibers (axons) were detected in 28% of invasive ductal carcinomas as compared to only 12% of invasive lobular carcinomas and 8% of ductal carcinomas in situ (p=0.0003). In invasive breast cancers, the presence of nerve fibers was observed in 15% of lymph node negative tumors and 28% of lymph node positive tumors (p=0.0031), indicating a relationship with the metastatic potential. In addition, there was an association between the presence of nerve fibers and the expression of nerve growth factor (NGF) in cancer cells (p=0.0001). Invitro, breast cancer cells were able to induce neurite outgrowth in PC12 cells, and this neurotrophic activity was partially inhibited by anti-NGF blocking antibodies. In conclusion, infiltration by nerve fibers is a feature of the tumor microenvironment that is associated with aggressiveness and involves NGF production by cancer cells. The potential participation of nerve fibers in breast cancer progression needs to be further considered.

DOI10.1016/j.molonc.2015.05.001
Co-authorsPhillip Jobling, John Attia, Rodney Scott, Hubert Hondermarck, Marjorie Walker
2015Ahmed AF, de Bock CE, Lincz LF, Pundavela J, Zouikr I, Sontag E, et al., 'FAT1 cadherin acts upstream of Hippo signalling through TAZ to regulate neuronal differentiation.', Cellular and molecular life sciences : CMLS, (2015)
Co-authorsHubert Hondermarck
2014Pundavela J, Demont Y, Jobling P, Lincz LF, Roselli S, Thorne RF, et al., 'ProNGF correlates with Gleason score and is a potential driver of nerve infiltration in prostate cancer', American Journal of Pathology, 184 3156-3162 (2014) [C1]

Nerve infiltration is essential to prostate cancer progression, but the mechanism by which nerves are attracted to prostate tumors remains unknown. We report that the precursor of... [more]

Nerve infiltration is essential to prostate cancer progression, but the mechanism by which nerves are attracted to prostate tumors remains unknown. We report that the precursor of nerve growth factor (proNGF) is overexpressed in prostate cancer and involved in the ability of prostate cancer cells to induce axonogenesis. A series of 120 prostate cancer and benign prostate hyperplasia (BPH) samples were analyzed by IHC for proNGF. ProNGF was mainly localized in the cytoplasm of epithelial cells, with marked expression in cancer compared with BPH. Importantly, the proNGF level positively correlated with the Gleason score (n = 104, tB = 0.51). A higher level of proNGF was observed in tumors with a Gleason score of =8 compared with a Gleason score of 7 and 6 (P < 0.001). In vitro, proNGF was detected in LNCaP, DU145, and PC-3 prostate cancer cells and BPH-1 cells but not in RWPE-1 immortalized nontumorigenic prostate epithelial cells or primary normal prostate epithelial cells. Co-culture of PC12 neuronal-like cells or 50B11 neurons with PC-3 cells resulted in neurite outgrowth in neuronal cells that was inhibited by blocking antibodies against proNGF, indicating that prostate cancer cells can induce axonogenesis via secretion of proNGF. These data reveal that ProNGF is a biomarker associated with high-risk prostate cancers and a potential driver of infiltration by nerves.

DOI10.1016/j.ajpath.2014.08.009
CitationsScopus - 2Web of Science - 1
Co-authorsPhillip Jobling, Hubert Hondermarck, Lisa Lincz, Marjorie Walker
2014Sadeqzadeh E, De Bock CE, Wojtalewicz N, Holt JE, Smith ND, Dun MD, et al., 'Furin processing dictates ectodomain shedding of human FAT1 cadherin', Experimental Cell Research, 323 41-55 (2014)
DOI10.1016/j.yexcr.2014.02.012
Co-authorsJanet Holt, Matt Dun
2014Sadeqzadeh E, De Bock CE, Wojtalewicz N, Holt JE, Smith ND, Dun MD, et al., 'Furin processing dictates ectodomain shedding of human FAT1 cadherin', Experimental Cell Research, 323 41-55 (2014) [C1]

Fat1 is a single pass transmembrane protein and the largest member of the cadherin superfamily. Mouse knockout models and in vitro studies have suggested that Fat1 influences cell... [more]

Fat1 is a single pass transmembrane protein and the largest member of the cadherin superfamily. Mouse knockout models and in vitro studies have suggested that Fat1 influences cell polarity and motility. Fat1 is also an upstream regulator of the Hippo pathway, at least in lower vertebrates, and hence may play a role in growth control. In previous work we have established that FAT1 cadherin is initially cleaved by proprotein convertases to form a noncovalently linked heterodimer prior to expression on the cell surface. Such processing was not a requirement for cell surface expression, since melanoma cells expressed both unprocessed FAT1 and the heterodimer on the cell surface. Here we further establish that the site 1 (S1) cleavage step to promote FAT1 heterodimerisation is catalysed by furin and we identify the cleavage site utilised. For a number of other transmembrane receptors that undergo heterodimerisation the S1 processing step is thought to occur constitutively but the functional significance of heterodimerisation has been controversial. It has also been generally unclear as to the significance of receptor heterodimerisation with respect to subsequent post-translational proteolysis that often occurs in transmembrane proteins. Exploiting the partial deficiency of FAT1 processing in melanoma cells together with furin-deficient LoVo cells, we manipulated furin expression to demonstrate that only the heterodimer form of FAT1 is subject to cleavage and subsequent release of the extracellular domain. This work establishes S1-processing as a clear functional prerequisite for ectodomain shedding of FAT1 with general implications for the shedding of other transmembrane receptors. © 2014.

DOI10.1016/j.yexcr.2014.02.012
CitationsScopus - 1Web of Science - 1
Co-authorsMatt Dun, Janet Holt
2014Sadeqzadeh E, de Bock CE, O'Donnell MR, Timofeeva A, Burns GF, Thorne RF, 'FAT1 cadherin is multiply phosphorylated on its ectodomain but phosphorylation is not catalysed by the four-jointed homologue', FEBS LETTERS, 588 3511-3517 (2014) [C1]
DOI10.1016/j.febslet.2014.08.014Author URL
2014Sadeqzadeh E, De Bock CE, Thorne RF, 'Sleeping Giants: Emerging Roles for the Fat Cadherins in Health and Disease', Medicinal Research Reviews, 34 190-221 (2014)
DOI10.1002/med.21286
CitationsScopus - 6
2014Sadeqzadeh E, De Bock CE, Thorne RF, 'Sleeping Giants: Emerging Roles for the Fat Cadherins in Health and Disease', Medicinal Research Reviews, 34 190-221 (2014) [C1]

The vertebrate Fat cadherins comprise a small gene family of four members, Fat1-Fat4, all closely related in structure to Drosophila ft and ft2. Over the past decade, knock-out mo... [more]

The vertebrate Fat cadherins comprise a small gene family of four members, Fat1-Fat4, all closely related in structure to Drosophila ft and ft2. Over the past decade, knock-out mouse studies, genetic manipulation, and large sequencing projects has aided our understanding of the function of vertebrate Fat cadherins in tissue development and disease. The majority of studies of this family have focused on Fat1, with evidence now showing it can bind enable (ENA)/Vasodilator-stimulated phosphoprotein (VASP), ß-catenin and Atrophin proteins to influence cell polarity and motility; HOMER-1 and HOMER-3 proteins to regulate actin accumulation in neuronal synapses; and scribble to influence the Hippo signaling pathway. Fat2 and Fat3 can regulate cell migration in a tissue specific manner and Fat4 appears to influence both planar cell polarity and Hippo signaling recapitulating the activity of Drosophila ft. Knowledge about the exact downstream signaling pathways activated by each family member remains in its infancy, but it is becoming clearer that they have tissue specific and redundant roles in development and may be lost or gained in cancer. In this review, we summarize the recent progress on understanding the role of the Fat cadherin family, integrating the current knowledge of molecular interactions and tissue distributions, together with the accumulating evidence of their changed expression in human disease. The latter is now beginning to promote interest in these molecules as both biomarkers and new targets for therapeutic intervention. © 2013 Wiley Periodicals, Inc.

DOI10.1002/med.21286
CitationsScopus - 7Web of Science - 6
2014Mhaidat NM, Bouklihacene M, Thorne RF, '5-Fluorouracil-induced apoptosis in colorectal cancer cells is caspase-9-dependent and mediated by activation of protein kinase C-d', Oncology Letters, 8 699-704 (2014) [C1]

Elucidation of the molecular mechanisms by which 5-fluorouracil (5-FU) induces apoptosis is required in order to understand the resistance of colorectal cancer (CRC) cells to 5-FU... [more]

Elucidation of the molecular mechanisms by which 5-fluorouracil (5-FU) induces apoptosis is required in order to understand the resistance of colorectal cancer (CRC) cells to 5-FU. In the current study, 5-FU-induced apoptosis was assessed using the propidium iodide method. Involvement of protein kinase C (PKC) was assessed by evaluating the extent of their activation in CRC, following treatment with 5-FU, using biochemical inhibitors and western blot analysis. The results revealed that 5-FU induces varying degrees of apoptosis in CRC cells; HCT116 cells were identified to be the most sensitive cells and SW480 were the least sensitive. In addition, 5-FU-induced apoptosis was caspase-dependent as it appeared to be initiated by caspase-9. Furthermore, PKCe was marginally expressed in CRC cells and no changes were observed in the levels of cleavage or phosphorylation following treatment with 5-FU. The treatment of HCT116 cells with 5-FU increased the expression, phosphorylation and cleavage of PKCd. The inhibition of PKCd was found to significantly inhibit 5-FU-induced apoptosis. These results indicated that 5-FU induces apoptosis in CRC by the activation of PKCd and caspase-9. In addition, the levels of PKCd activation may determine the sensitivity of CRC to 5-FU.

DOI10.3892/ol.2014.2211
CitationsScopus - 2Web of Science - 1
2014Vuong QV, Sadeqzadeh E, Hirun S, Goldsmith CD, Zammitt N, Bowyer MB, et al., 'Phenolic Compounds, Antioxidant and Anti-Cancer Properties of the Australian Maroon Bush Scaevola spinescens (Goodeniaceae)', Journal of Bioanalysis & Biomedicine, S12 (2014) [C1]
DOI10.4172/1948-593X.S12-002
Co-authorsMichael Bowyer, Vanquan Vuong, Judith Weidenhofer, C Scarlett, Jennette Sakoff
2014Wojtalewicz N, Sadeqzadeh E, Weiss JV, Tehrani MM, Klein-Scory S, Hahn S, et al., 'A Soluble Form of the Giant Cadherin Fat1 Is Released from Pancreatic Cancer Cells by ADAM10 Mediated Ectodomain Shedding', PLOS ONE, 9 (2014) [C1]
DOI10.1371/journal.pone.0090461Author URL
2014Oliveira CS, de Bock CE, Molloy TJ, Sadeqzadeh E, Geng XY, Hersey P, et al., 'Macrophage migration inhibitory factor engages PI3K/Akt signalling and is a prognostic factor in metastatic melanoma', BMC Cancer, 14 (2014) [C1]

Background: Macrophage migration inhibitory factor (MIF) is a widely expressed cytokine involved in a variety of cellular processes including cell cycle regulation and the control... [more]

Background: Macrophage migration inhibitory factor (MIF) is a widely expressed cytokine involved in a variety of cellular processes including cell cycle regulation and the control of proliferation. Overexpression of MIF has been reported in a number of cancer types and it has previously been shown that MIF is upregulated in melanocytic tumours with the highest expression levels occurring in malignant melanoma. However, the clinical significance of high MIF expression in melanoma has not been reported. Methods: MIF expression was depleted in human melanoma cell lines using siRNA-mediated gene knockdown and effects monitored using in vitro assays of proliferation, cell cycle, apoptosis, clonogenicity and Akt signalling. In silico analyses of expression microarray data were used to correlate MIF expression levels in melanoma tumours with overall patient survival using a univariate Cox regression model. Results: Knockdown of MIF significantly decreased proliferation, increased apoptosis and decreased anchorage-independent growth. Effects were associated with reduced numbers of cells entering S phase concomitant with decreased cyclin D1 and CDK4 expression, increased p27 expression and decreased Akt phosphorylation. Analysis of clinical outcome data showed that MIF expression levels in primary melanoma were not associated with outcome (HR = 1.091, p = 0.892) whereas higher levels of MIF in metastatic lesions were significantly associated with faster disease progression (HR = 2.946, p = 0.003 and HR = 4.600, p = 0.004, respectively in two independent studies). Conclusions: Our in vitro analyses show that MIF functions upstream of the PI3K/Akt pathway in human melanoma cell lines. Moreover, depletion of MIF inhibited melanoma proliferation, viability and clonogenic capacity. Clinically, high MIF levels in metastatic melanoma were found to be associated with faster disease recurrence. These findings support the clinical significance of MIF signalling in melanoma and provide a strong rationale for both targeting and monitoring MIF expression in clinical melanoma.

DOI10.1186/1471-2407-14-630
Co-authorsXu Zhang
2013Li J, Imtiaz MS, Beard NA, Dulhunty AF, Thorne R, vanHelden DF, Laver DR, 'ß-Adrenergic stimulation increases RyR2 activity via intracellular Ca2+ and Mg2+ regulation.', PLoS One, 8 e58334 (2013) [C1]
DOI10.1371/journal.pone.0058334Author URL
Co-authorsDerek Laver, Dirk Vanhelden
2013Mhaidat NM, Qandil AM, Al-Balas QA, Hassan MA, Jaradat SA, Matalkah AM, Thorne RT, 'Methoxyphenylcipro induces antitumor activity in human cancer cells', Archives of Pharmacal Research, 36 1023-1028 (2013) [C1]
DOI10.1007/s12272-013-0087-5Author URL
2013Guo ST, Jiang CC, Wang GP, Li YP, Wang CY, Guo XY, et al., 'MicroRNA-497 targets insulin-like growth factor 1 receptor and has a tumour suppressive role in human colorectal cancer', ONCOGENE, 32 1910-1920 (2013) [C1]
DOI10.1038/onc.2012.214Author URL
CitationsScopus - 41Web of Science - 34
Co-authorsXu Zhang
2013Ardjmand A, de Bock CE, Shahrokhi S, Lincz LF, Boyd AW, Burns GF, Thorne RF, 'Fat1 cadherin provides a novel minimal residual disease marker in acute lymphoblastic leukemia', HEMATOLOGY, 18 315-321 (2013) [C1]
DOI10.1179/1607845413Y.0000000080Author URL
Co-authorsLisa Lincz
2013Ye Y, Jin L, Wilmott JS, Hu WL, Yosufi B, Thorne RF, et al., 'PI(4,5)P2 5-phosphatase A regulates PI3K/Akt signalling and has a tumour suppressive role in human melanoma', NATURE COMMUNICATIONS, 4 (2013) [C1]
DOI10.1038/ncomms2489Author URL
CitationsScopus - 15Web of Science - 15
Co-authorsXu Zhang
2013Alkhatatbeh MJ, Enjeti AK, Acharya S, Thorne RF, Lincz LF, 'The origin of circulating CD36 in type 2 diabetes', NUTRITION & DIABETES, 3 (2013) [C1]
DOI10.1038/nutd.2013.1Author URL
CitationsScopus - 8Web of Science - 6
Co-authorsLisa Lincz
2012De Bock CE, Ardjmand Ghahestani A, Molloy TJ, Bone SM, Johnstone DM, Campbell DM, et al., 'The Fat1 cadherin is overexpressed and an independent prognostic factor for survival in paired diagnosis-relapse samples of precursor B-cell acute lymphoblastic leukemia', Leukemia, 26 918-926 (2012) [C1]
CitationsScopus - 11Web of Science - 12
Co-authorsLisa Lincz
2012Mhaidat NM, Abdul-Razzak KK, Alkofahi AS, Alsarhan AM, Aldaher AN, Thorne RF, 'Altholactone induces apoptotic cell death in human colorectal cancer cells', Phytotherapy Research, 26 926-931 (2012) [C1]
CitationsScopus - 4Web of Science - 3
2012Tay KH, Jin L, Tseng HY, Jiang CC, Ye Y, Thorne RF, et al., 'Suppression of PP2A is critical for protection of melanoma cells upon endoplasmic reticulum stress', Cell Death and Disease, 3 e337 (2012) [C1]
CitationsScopus - 15Web of Science - 15
Co-authorsXu Zhang, Nikki Verrills
2011Alkhatatbeh MJ, Mhaidat NM, Enjeti AK, Lincz L, Thorne RF, 'The putative diabetic plasma marker, soluble CD36, is non-cleaved, non-soluble and entirely associated with microparticles', Journal of Thrombosis and Haemostasis, 9 844-851 (2011) [C1]
DOI10.1111/j.1538-7836.2011.04220.x
CitationsScopus - 16Web of Science - 16
Co-authorsLisa Lincz
2011Jiang CC, Lai F, Thorne RF, Yang F, Liu H, Hersey P, Zhang XD, 'MEK-independent survival of B-RAFV600E melanoma cells selected for resistance to apoptosis induced by the RAF inhibitor PLX4720', Clinical Cancer Research, 17 721-730 (2011) [C1]
CitationsScopus - 58Web of Science - 56
Co-authorsXu Zhang
2011Sadeqzadeh E, De Bock CE, Zhang XD, Shipman KL, Scott NM, Song C, et al., 'Dual processing of FAT1 cadherin protein by human melanoma cells generates distinct protein products', Journal of Biological Chemistry, 286 28181-28191 (2011) [C1]
DOI10.1074/jbc.M111.234419
CitationsScopus - 13Web of Science - 12
Co-authorsXu Zhang
2011Jin L, Hu WL, Jiang CC, Wang JX, Han CC, Chu P, et al., 'MicroRNA-149*, a p53-responsive microRNA, functions as an oncogenic regulator in human melanoma', Proceedings of the National Academy of Sciences, 108 15840-15845 (2011) [C1]
CitationsScopus - 58Web of Science - 58
Co-authorsXu Zhang
2011Dong L, Jiang CC, Thorne RF, Croft A, Yang F, Liu H, et al., 'Ets-1 mediates upregulation of Mcl-1 downstream of XBP-1 in human melanoma cells upon ER stress', Oncogene, 30 3716-3726 (2011) [C1]
DOI10.1038/onc.2011.87
CitationsScopus - 28Web of Science - 27
Co-authorsXu Zhang
2010Tseng H-Y, Jiang CC, Croft A, Croft A, Thorne RF, Yang F, et al., 'Contrasting effects of Nutlin-3 on TRAIL - and Docetaxel-induced Apoptosis due to upregulation of TRAIL-R2 and Mcl-1 in human melanoma cells', Molecular Cancer Therapeutics, 9 3363-3374 (2010) [C1]
DOI10.1158/1535-7163.MCT-10-0646
Co-authorsXu Zhang
2010Zhang X, Thorne RF, Wagner TE, Wei Y, 'Regulatory T cells and cancer therapy: An old story with a new hope', Current Cancer Therapy Reviews, 6 34-40 (2010) [C1]
DOI10.2174/157339410790596470
2010Jiang CC, Lai F, Tay KH, Croft A, Rizos H, Becker TM, et al., 'Apoptosis of human melanoma cells induced by inhibition of B-RAF(V600E) involves preferential splicing of bim(S)', Cell Death & Disease, 1 e69 (2010) [C1]
DOI10.1038/cddis.2010.48
CitationsScopus - 50Web of Science - 50
Co-authorsXu Zhang
2010Thorne RF, Ralston KJ, De Bock CE, Mhaidat NM, Zhang XD, Boyd AW, Burns GF, 'Palmitoylation of CD36/FAT regulates the rate of its post-transcriptional processing in the endoplasmic reticulum', Biochimica et Biophysica Acta - Molecular Cell Research, 1803 1298-1307 (2010) [C1]
DOI10.1016/j.bbamcr.2010.07.002
CitationsScopus - 9Web of Science - 9
Co-authorsXu Zhang
2010Yang F, Tay KH, Dong L, Thorne RF, Jiang CC, Yang E, et al., 'Cystatin B inhibition of TRAIL-induced apoptosis is associated with the protection of FLIPL from degradation by the E3 ligase itch in human melanoma cells', Cell Death and Differentiation, 17 1354-1367 (2010) [C1]
DOI10.1038/cdd.2010.29
CitationsScopus - 29Web of Science - 26
Co-authorsXu Zhang
2010Mao ZG, Jiang CC, Thorne RF, Hersey P, Zhang XD, 'TRAIL-induced apoptosis of human melanoma cells involves activation of caspase-4', Apoptosis, 15 1211-1222 (2010) [C1]
DOI10.1007/s10495-010-0513-9
CitationsScopus - 12Web of Science - 10
Co-authorsXu Zhang
2009Jiang CC, Yang F, Thorne RF, Zhu BK, Hersey P, Zhang XD, 'Human melanoma cells under endoplasmic reticulum stress acquire resistance to microtubule-targeting drugs through XBP-1-mediated activation of Akt', Neoplasia, 11 436-447 (2009) [C1]
DOI10.1593/neo.09208
CitationsScopus - 28Web of Science - 26
Co-authorsXu Zhang
2009Zhang LJ, Chen S, Wu P, Hu CS, Thorne RF, Luo CM, et al., 'Inhibition of MEK blocks GRP78 up-regulation and enhances apoptosis induced by ER stress in gastric cancer cells', Cancer Letters, 274 40-46 (2009) [C1]
DOI10.1016/j.canlet.2008.08.030
CitationsScopus - 24Web of Science - 22
Co-authorsXu Zhang
2009Mhaidat NM, Alali FQ, Matalqah SM, Matalka II, Jaradat SA, Al-Sawalha NA, Thorne RF, 'Inhibition of MEK sensitizes paclitaxel-induced apoptosis of human colorectal cancer cells by downregulation of GRP78', Anti-Cancer Drugs, 20 601-606 (2009) [C1]
DOI10.1097/cad.0b013e32832e3120
CitationsScopus - 12Web of Science - 13
2008Mhaidat NM, Thorne RF, Zhang XD, Hersey P, 'Involvement of endoplasmic reticulum stress in Docetaxel-induced JNK-dependent apoptosis of human melanoma', Apoptosis, 13 1505-1512 (2008) [C1]
DOI10.1007/s10495-008-0276-8
CitationsScopus - 17Web of Science - 16
Co-authorsXu Zhang
2008Chen LH, Jiang CC, Watts R, Thorne RF, Kiejda KA, Zhang XD, Hersey P, 'Inhibition of endoplasmic reticulum stress-induced apoptosis of melanoma cells by the ARC protein', Cancer Research, 68 834-842 (2008) [C1]
DOI10.1158/0008-5472.can-07-5056
CitationsScopus - 27Web of Science - 24
Co-authorsKelly Kiejda, Xu Zhang
2008Jiang CC, Lucas K, Kiejda KA, Wade M, Debock CE, Thorne RF, et al., 'Up-regulation of Mcl-1 is critical for survival of human melanoma cells upon endoplasmic reticulum stress', Cancer Research, 68 6708-6717 (2008) [C1]
DOI10.1158/0008-5472.can-08-0349
CitationsScopus - 73Web of Science - 69
Co-authorsXu Zhang, Kelly Kiejda
2008Mhaidat NM, Thorne RF, De Bock CE, Zhang XD, Hersey P, 'Melanoma cell sensitivity to docetaxal-induced apoptosis is determined by class III beta-tubulin levels', FEBS Letters, 582 267-272 (2008) [C1]
DOI10.1016/j.febslet.2007.12.014
CitationsScopus - 9Web of Science - 8
Co-authorsXu Zhang
2008Mhaidat NM, Thorne RF, De Bock CE, Zhang XD, Hersey P, 'Melanoma cell sensitivity to docetaxal-induced apoptosis is determined by class III beta-tubulin levels', FEBS Letters, 582 267-272 (2008) [C1]
DOI10.1016/j.febslet.2007.12.014
Co-authorsXu Zhang
2008Zhang LJ, Hao YZ, Hu CS, Ye Y, Xie QP, Thorne RF, et al., 'Inhibition of apoptosis facilitates necrosis induced by cisplatin in gastric cancer cells', Anti-Cancer Drugs, 19 159-166 (2008) [C1]
DOI10.1097/CAD.0b013e3282f30d05
CitationsScopus - 6Web of Science - 7
Co-authorsXu Zhang
2007Mhaidat NM, Thorne RF, Zhang XD, Hersey P, 'Regulation of docetaxel-induced apoptosis of human melanoma cells by different isoforms of protein kinase C', Molecular Cancer Research, 5 1073-1081 (2007) [C1]
DOI10.1158/1541-7786.mcr-07-0059
CitationsScopus - 21Web of Science - 19
Co-authorsXu Zhang
2007Thorne RF, Mhaidat N, Ralston KJ, Burns GF, 'Shed gangliosides provide detergent-independent evidence for type-3 glycosynapses', Biochemical and Biophysical Research Communications, 356 306-311 (2007) [C1]
DOI10.1016/j.bbrc.2007.02.139
CitationsScopus - 5Web of Science - 5
2007Jiang CC, Li HC, Gillespie S, Kiejda KA, Mhaidat N, Yu FW, et al., 'Tunicamycin sensitizes human melanoma cells to tumor necrosis factor-related apoptosis-inducing ligand-induced apoptosis by up-regulation of TRAIL-R2 via the unfolded protein response', Cancer Research, 67 5880-5888 (2007) [C1]
DOI10.1158/0008-5472.CAN-07-0213
CitationsScopus - 61Web of Science - 61
Co-authorsXu Zhang, Kelly Kiejda
2007Thorne RF, Mhaidat N, Ralston KJ, Burns GF, 'CD36 is a receptor for oxidized high density lipoprotein: Implications for the development of atherosclerosis', FEBS Letters, 581 1227-1232 (2007) [C1]
DOI10.1016/j.febslet.2007.02.043
CitationsScopus - 38Web of Science - 36
2007Chen LH, Jiang CC, Kiejda KA, Wang YF, Thorne RF, Zhang XD, Hersey P, 'Thapsigargin sensitizes human melanoma cells to TRAIL-induced apoptosis by up-regulation of TRAIL-R2 through the unfolded protein response', Carcinogenesis, 28 2328-2336 (2007) [C1]
DOI10.1093/carcin/bgm173
CitationsScopus - 30Web of Science - 24
Co-authorsXu Zhang, Kelly Kiejda
2006Tzircotis G, Thorne RF, Isacke CM, 'Directional sensing of a phorbol ester gradient requires CD44 and is regulated by CD44 phosphorylation', Oncogene, 25 7401-7410 (2006) [C1]
DOI10.1038/sj.onc.1209724
CitationsScopus - 11Web of Science - 11
2006Thorne RF, Zhang XH, Song CJ, Jin BQ, Burns GF, 'Novel immunoblotting monoclonal antibodies against human and rat CD36/fat used to identify an isoform of CD36 in rat muscle', DNA and Cell Biology, 25 302-311 (2006) [C1]
DOI10.1089/dna.2006.25.302
CitationsScopus - 6Web of Science - 6
2006Thorne RF, Law E, Elith CA, Ralston KJ, Bates RC, Burns GF, 'The association between CD36 and Lyn protein tyrosine kinase is mediated by lipid', Biochemical and Biophysical Research Communications, 351 51-56 (2006) [C1]
DOI10.1016/j.bbrc.2006.09.156
CitationsScopus - 10Web of Science - 8
2005Tzircotis G, Thorne RF, Isacke CM, 'Chemotaxis towards hyaluronan is dependent on CD44 expression and modulated by cell type variation in CD44-hyaluronan binding', Journal of Cell Science, 118 5119-5128 (2005) [C1]
DOI10.1242/jcs.02629
CitationsScopus - 52Web of Science - 51
2004Ralston KJ, Hird S, Zhang X, Scott JL, Jin B, Thorne RF, et al., 'The LFA-1-associated molecule PTA-1 (CD226) on T cells forms a dynamic molecular complex with protein 4.1G and human discs large', Journal of Biological Chemistry, 279 33816-33828 (2004) [C1]
DOI10.1074/jbc.M401040200
CitationsScopus - 43Web of Science - 40
2004Thorne RF, Legg JW, Isacke CM, 'The role of the CD44 transmembrane and cytoplasmic domains in co-ordinating adhesive and signalling events', JOURNAL OF CELL SCIENCE, 117 373-380 (2004) [C1]
DOI10.1242/jcs.00954Author URL
CitationsScopus - 104Web of Science - 96
2003Sullivan A, Uff CR, Isacke CM, Thorne RF, 'PACE-1, a novel protein that interacts with the C-terminal domain of ezrin', EXPERIMENTAL CELL RESEARCH, 284 224-238 (2003) [C1]
DOI10.1016/S0014-4827(02)00054-XAuthor URL
CitationsScopus - 5Web of Science - 5
2000Gruarin P, Thorne R, Dorahy DJ, Burns G, Sitia R, Alessio M, 'CD36 is a Ditopic Glycoprotein with the N-Terminal Domain Implicated in Intracellular Transport', Biochemical and Biophysical Research Communications, 275 446-454 (2000) [C1]
CitationsScopus - 31Web of Science - 30
2000Thorne R, Marshall J, Shafren D, Gibson PG, Hart I, Burns G, 'The Integrins a3B1 and a6B1 Physically and Functionally Associate with CD36 in Human Melanoma Cells', Journal of Biological Chemistry, 275 35264-35275 (2000) [C1]
CitationsScopus - 67Web of Science - 66
Co-authorsPeter Gibson
2000Thorne RF, Marshall J, Shafren D, Gibson P, Hart I, Burns GF, 'The Integrins (alpha)3(beta)1 Physically and functionally Associate with CD36 in Human Melanoma Cells', The Journal of Biological Chemistry, 275 No. 45 35264-35275 (2000) [C1]
2000Shafren D, Dorahy DJ, Thorne R, Barry R, 'Cytoplasmic interactions between decay-accelerating factor and intercellular adhesion molecule-1 are not required for coxsackievirus A21 cell infection', Journal of General Virology, 81 889-894 (2000) [C1]
CitationsScopus - 17Web of Science - 14
2000Gruarin P, Ulliers D, Thorne RF, Alessio M, 'Methionine 156 in the immunodominant domain on CD36 contributes to define the epitope recognized by the NL07 MoAb', Molecular and Cellular Biochemistry, 214 89-95 (2000) [C1]
CitationsScopus - 1Web of Science - 1
1998Gruarin P, Sitia R, Thorne RF, Burns GF, Alessio M, 'CD36 is a ditopic glycoprotein with both transmembrane domains implicated in intracellular transport.', MOLECULAR BIOLOGY OF THE CELL, 9 456A-456A (1998)
Author URL
1998Bates RC, Elith CA, Thorne RF, Burns GF, 'Engagement of Variant CD44 confers resistance to anti-integrin antibody-mediated apoptosis in a colon carcinoma cell line', Cell Adhesion and Communication, 6 (1) 21-38 (1998) [C1]
CitationsScopus - 20Web of Science - 21
1998Shafren DR, Dorahy DJ, Thorne RF, Kinoshita T, Barry RD, Burns GF, 'Antibody binding to individual short consensus repeats of decay-accelerating factor enhances enterovirus cell attachment and infectivity', The Journal of Immunology, 160 2318-2323 (1998) [C1]
CitationsScopus - 22Web of Science - 22
1997Thorne RF, Meldrum CJ, Harris SJ, Dorahy DJ, Shafren DR, Berndt MC, et al., 'CD36 forms covalently associated dimers and multimers in platelets and transfected COS-7 cells', BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 240 812-818 (1997)
DOI10.1006/bbrc.1997.7755Author URL
CitationsScopus - 25Web of Science - 24
Co-authorsPeter Gibson
1997Dorahy DJ, Thorne RF, Fecondo JV, Burns GF, 'Stimulation of platelet activation and aggregation by a carboxyl-terminal peptide from thrombospondin binding to the integrin-associated protein receptor', JOURNAL OF BIOLOGICAL CHEMISTRY, 272 1323-1330 (1997)
Author URL
CitationsScopus - 44Web of Science - 43
1997Radford KJ, Thorne RF, Hersey P, 'Regulation of tumor cell motility and migration by CD63 in a human melanoma cell line', JOURNAL OF IMMUNOLOGY, 158 3353-3358 (1997)
Author URL
CitationsScopus - 67Web of Science - 65
1996Radford KJ, Thorne RF, Hersey P, 'CD63 associates with transmembrane 4 superfamily members, CD9 and CD81, and with beta 1 integrins in human melanoma', BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 222 13-18 (1996)
DOI10.1006/bbrc.1996.0690Author URL
CitationsScopus - 66Web of Science - 66
1989SMART YC, STEVENSON KL, THORNE RF, THOMAS WD, HSU LH, BURTON RC, 'EXPRESSION OF NATURAL-KILLER (NK) CELL-SPECIFIC ALLOANTIGENS ON A MOUSE NK-LIKE CELL-LINE', IMMUNOLOGY AND CELL BIOLOGY, 67 239-242 (1989)
DOI10.1038/icb.1989.36Author URL
CitationsScopus - 3Web of Science - 5
Show 64 more journal articles

Conference (18 outputs)

YearCitationAltmetricsLink
2014Faulkner S, Roselli S, Thorne RF, Scarlett CJ, Walker MM, Hondermarck H, 'PRONGF AND SORTILIN EXPRESSION AND FUNCTION IN PANCREATIC CANCER', ASIA-PACIFIC JOURNAL OF CLINICAL ONCOLOGY (2014) [E3]
Author URL
Co-authorsC Scarlett, Hubert Hondermarck, Marjorie Walker
2014Sadeqzadeh E, Vuong QV, Goldsmith CD, Nguyen VT, Bhuyan DJ, Trung TD, et al., 'A NATURAL PRODUCT DRUG DISCOVERY PIPELINE FOR NOVEL PANCREATIC CANCER THERAPIES: A NEW CANCER RESEARCH HUB FOR THE HUNTER REGION OF NSW', ASIA-PACIFIC JOURNAL OF CLINICAL ONCOLOGY (2014) [E3]
Author URL
Co-authorsTroy Gaston, Michael Bowyer, Anita Chalmers, Vanquan Vuong, Judith Weidenhofer, C Scarlett, Natalie Moltschaniwskyj
2014Guo ST, Chi MN, Yang RH, Guo XY, Wang CY, Zan LQ, et al., 'INOSITOL POLYPHOSPHATE 4-PHOSPHATASE II PROMOTES PI3K SIGNALING AND FUNCTIONS AS AN ONCOGENIC REGULATOR IN HUMAN COLON CANCER', ASIA-PACIFIC JOURNAL OF CLINICAL ONCOLOGY (2014) [E3]
Author URL
Co-authorsXu Zhang, Rodney Scott, Stephen Ackland
2013Ahmed AF, De Bock CE, Sontag E, Hondermarck H, Thorne RF, 'The functional role of Fat1 cadherin in the differentiation and proliferation of SH-SY5Y neuroblastoma cells', -, Pokolbin, NSW, Australia (2013) [E3]
Co-authorsEstelle Sontag, Hubert Hondermarck
2012Li J, Imtiaz MS, Beard NA, Dulhunty AF, Thorne RF, Van Helden DF, Laver DR, 'Adrenergic stimulation increases RYR2 activity via intracellular Ca2+ and Mg2+ regulation', Sydney 2012 Joint AuPS/PSNZ/ASB Meeting. Programme, Sydney, NSW (2012) [E3]
CitationsScopus - 6Web of Science - 6
Co-authorsDirk Vanhelden, Derek Laver
2012Li J, Imtiaz MS, Beard NA, Dulhunty AF, Thorne RF, Van Helden DF, Laver DR, 'Adrenergic stimulation alters RyR2 regulation by Ca2+ and Mg2+', Heart, Lung and Circulation: Abstracts of ISHR Auckland Workshop, Auckland, NZ (2012) [E3]
Co-authorsDirk Vanhelden, Derek Laver
2011Ye Y, Jin L, Wilmott J, Hu WL, Thorne RF, Dong L, et al., 'Phosphatidylinositol 4,5-Bisphosphate 5-Phosphatase A regulates PI3K/Akt signaling in human melanoma cells', Pigment Cell & Melanoma Research, Tampa, FL (2011) [E3]
DOI10.1111/j.1755-148X.2011.00909.x
Co-authorsXu Zhang
2011Alkhatatbeh MJ, Mhaidat NM, Enjeti AK, Lincz LF, Thorne RF, 'Circulating plasma CD36+microparticles as a marker of type 2 diabetes and its complications', Clinical Biochemistry, Mashhad, Iran (2011) [E3]
Co-authorsLisa Lincz
2011Ardjmand Ghahestani A, De Bock CE, Molloy TJ, Bone SM, Johnstone DM, Campbell DM, et al., 'Altered expression of Fat1 cadherin, a novel tumor marker for acute lymphoblastic leukemia', Clinical Biochemistry, Mashhad, Iran (2011) [E3]
Co-authorsLisa Lincz
2011Sadeqzadeh E, Hersey P, Zhang XD, Debock C, Boyd A, Burns GF, Thorne RF, 'Aberrant processing of Fat1 Cadherin in human cancer', Clinical Biochemistry, Mashhad, Iran (2011) [E3]
Co-authorsXu Zhang
2010Dong L, Jiang CC, Thorne RF, Yang F, Liu H, De Bock CE, et al., 'Transcriptional up-regulation of Mcl-1 by ETS1 down-stream of XBP-1 in melanoma cells upon ER stress', Pigment Cell & Melanoma Research, Sydney, Australia (2010) [E3]
Co-authorsXu Zhang
2010Chen LH, Yang F, Tay KH, Dong L, Thorne RF, Jiang CC, et al., 'Cystatin B inhibition of TRAIL-induced apoptosis is associated with protection of FLIPLfrom degradation by the E3 ligase itch human melanoma cells', Pigment Cell & Melanoma Research, Sydney, Australia (2010) [E3]
DOI10.1038/cdd.2010.29
Co-authorsXu Zhang
2010Yang XM, Chen LH, Jiang CC, De Bock CE, Thorne RF, Hersey P, Zhang XD, '40p53 is up-regulated and plays a role in antagonizing p53-mediated apoptosis in human melanoma', Pigment Cell & Melanoma Research, Sydney, Australia (2010) [E3]
Co-authorsXu Zhang
2010Zhang XD, Jiang CC, Lai F, Croft A, Tay KH, Thorne RF, et al., 'Apoptotic response of mutant B-RAF human melanoma cells to a B-RAF inhibitor involves increased splicing production of BimS', AACR 101st Annual Meeting 2010. Abstracts, Washington, DC (2010) [E3]
Co-authorsXu Zhang
2010Salum De Oliveira C, Yan XG, Hersey P, Zhang XD, Thorne RF, 'The role of MIF signaling in melanoma progression', AACR 101st Annual Meeting 2010. Abstracts, Washington, DC (2010) [E3]
Co-authorsXu Zhang
2010Jiang CC, Zhuang LQ, Dong L, Thorne RF, Lavis CJ, Hersey P, Zhang XD, 'Adaptation to ER stress as a driver of increased expression of Mcl-1 with melanoma progression', AACR 101st Annual Meeting 2010. Abstracts, Washington, DC (2010) [E3]
Co-authorsXu Zhang
2010Tseng HY, Jiang CC, Croft A, Tay KH, Yang F, Liu H, et al., 'Contrasting effects of nutlin-3 on TRAIL- and docetaxel-induced apoptosis in human melanoma cells', Melanoma 2010 Congress. Oral and Poster Abstracts, Sydney, NSW (2010) [E3]
DOI10.1158/1535-7163.MCT-10-0646
CitationsScopus - 17Web of Science - 17
Co-authorsXu Zhang
2001Law E, Thorne RF, Zhang XY, Burns GF, Boyd A, 'Fat Protocadherin as a Mechanical Regulator of Melanoma Cell Migration', Melanoma Research, Venice - 28 February to 3 March 2001 (2001) [E3]
Show 15 more conferences
Edit

Grants and Funding

Summary

Number of grants40
Total funding$5,875,982

Click on a grant title below to expand the full details for that specific grant.


20143 grants / $353,114

High Throughput Image Capture Platform for Translational Cancer Research$282,614

Funding body: Cancer Institute NSW

Funding bodyCancer Institute NSW
Project TeamConjoint Professor Stephen Ackland, Professor Rodney Scott, Professor John Forbes, Professor Xu Dong Zhang, Professor Marjorie Walker, Professor Hubert Hondermarck, Doctor Craig Gedye, Doctor Rick Thorne, Mr Loui Rassam, Doctor Stephen Braye
SchemeResearch Equipment Grant
RoleInvestigator
Funding Start2014
Funding Finish2014
GNoG1400626
Type Of FundingOther Public Sector - State
Category2OPS
UONY

What is different about red blood cells in people with type 2 diabetes?$50,000

Funding body: Hunter Medical Research Institute

Funding bodyHunter Medical Research Institute
Project TeamDoctor Lisa Lincz, Doctor Rick Thorne
SchemeProject Grant
RoleInvestigator
Funding Start2014
Funding Finish2014
GNoG1401416
Type Of FundingGrant - Aust Non Government
Category3AFG
UONY

Identification and evaluation of anti-pancreatic cancer activity of cytotoxic compounds extracted from Australian sea sponges: a pilot study$20,500

Funding body: Hunter Medical Research Institute

Funding bodyHunter Medical Research Institute
Project TeamDoctor Christopher Scarlett, Doctor Quan Vuong, Doctor Jude Weidenhofer, Doctor Rick Thorne, Associate Professor Michael Bowyer, Doctor Troy Gaston
SchemeProject Grant
RoleInvestigator
Funding Start2014
Funding Finish2014
GNoG1401452
Type Of FundingGrant - Aust Non Government
Category3AFG
UONY

20133 grants / $385,846

Functional consequences of epigenetic repression of PIB5PA in melanoma$359,250

Funding body: Cancer Council NSW

Funding bodyCancer Council NSW
Project TeamProfessor Xu Dong Zhang, Dr Helen Rizos, Doctor Rick Thorne, Doctor Chen Chen Jiang
SchemeResearch Grant
RoleInvestigator
Funding Start2013
Funding Finish2013
GNoG1200386
Type Of FundingGrant - Aust Non Government
Category3AFG
UONY

Keystone Meeting: The Hippo Tumor Suppressor Network, Monterey USA, 19-23 May 2013$2,000

Funding body: University of Newcastle - Faculty of Health and Medicine

Funding bodyUniversity of Newcastle - Faculty of Health and Medicine
Project TeamDoctor Rick Thorne
SchemeTravel Grant
RoleLead
Funding Start2013
Funding Finish2013
GNoG1300652
Type Of FundingInternal
CategoryINTE
UONY

20125 grants / $564,368

Targeting Histone Deacetylases to Overcome Resistance of BRAFV600E Melanoma Cells to Apoptosis$346,974

Funding body: NHMRC (National Health & Medical Research Council)

Funding bodyNHMRC (National Health & Medical Research Council)
Project TeamProfessor Xu Dong Zhang, Conjoint Professor Peter Hersey, Dr Tao Liu, Doctor Chen Chen Jiang, Doctor Rick Thorne
SchemeProject Grant
RoleInvestigator
Funding Start2012
Funding Finish2012
GNoG1100133
Type Of FundingAust Competitive - Commonwealth
Category1CS
UONY

High-Resolution Isoelectric Phosphoprotein Signalling System for Signalling Research, Biomarker Validation and Drug Development – Equipment Grant$143,394

Funding body: Hunter Medical Research Institute

Funding bodyHunter Medical Research Institute
Project TeamDoctor Rick Thorne, Professor Xu Dong Zhang, Professor Hubert Hondermarck, Conjoint Professor Stephen Ackland, Doctor Lisa Lincz, Doctor Jennette Sakoff, Emeritus Professor Leonie Ashman
SchemeProject Grant
RoleLead
Funding Start2012
Funding Finish2012
GNoG1200555
Type Of FundingContract - Aust Non Government
Category3AFC
UONY

Microscopic illumination system for advanced fluorescent protein technology$34,000

Funding body: NHMRC (National Health & Medical Research Council)

Funding bodyNHMRC (National Health & Medical Research Council)
Project TeamDoctor Rick Thorne, Professor Xu Dong Zhang, Conjoint Associate Professor Murray Cairns, Doctor Nikki Verrills, Doctor Charles De Bock, Doctor Jude Weidenhofer, Doctor Severine Roselli, Doctor Kathryn Skelding, Emeritus Professor Leonie Ashman, Professor Hubert Hondermarck
SchemeEquipment Grant
RoleLead
Funding Start2012
Funding Finish2012
GNoG1100983
Type Of FundingOther Public Sector - Commonwealth
Category2OPC
UONY

Establishment of Fat1 cadherin as biomarker and unique target for anti-cancer therapy in paediatric acute lymphoblastic leukaemia$20,000

Funding body: Hunter Medical Research Institute

Funding bodyHunter Medical Research Institute
Project TeamDoctor Rick Thorne, Doctor Charles De Bock, Doctor Lisa Lincz
SchemeResearch Grant
RoleLead
Funding Start2012
Funding Finish2012
GNoG1200222
Type Of FundingContract - Aust Non Government
Category3AFC
UONY

A p53-Mediated Pro-Survival Signaling Pathway in Human Melanoma Progression and Resistance to Treatment$20,000

Funding body: University of Newcastle

Funding bodyUniversity of Newcastle
Project TeamProfessor Xu Dong Zhang, Doctor Rick Thorne, Doctor Chen Chen Jiang
SchemeNear Miss Grant
RoleInvestigator
Funding Start2012
Funding Finish2012
GNoG1200672
Type Of FundingInternal
CategoryINTE
UONY

20111 grants / $10,000

IMPLEN NanoPhotometer pearl$10,000

Funding body: NHMRC (National Health & Medical Research Council)

Funding bodyNHMRC (National Health & Medical Research Council)
Project TeamConjoint Associate Professor Murray Cairns, Associate Professor Paul Tooney, Professor Alan Brichta, Emeritus Professor John Rostas, Emeritus Professor Patricia Michie, Conjoint Professor Keith Jones, Professor Ulli Schall, Associate Professor Phillip Dickson, Doctor Frederick Walker, Doctor Rick Thorne, Doctor Chris Dayas, Doctor Nikki Verrills, Doctor Janet Holt, Doctor Severine Roselli, Doctor Kathryn Skelding, Doctor Jude Weidenhofer, Associate Professor Liz Milward, Doctor Charles De Bock, Doctor Julie Merriman-Jones, Doctor Jing Qin Wu, Doctor Bing Liu, Mr Dan Johnstone, Ms Belinda Goldie, Doctor Natalie Beveridge
SchemeEquipment Grant
RoleInvestigator
Funding Start2011
Funding Finish2011
GNoG1100030
Type Of FundingOther Public Sector - Commonwealth
Category2OPC
UONY

20106 grants / $728,079

Pathogenic Function of Plasma CD36 Mircroparticles in Mediating Complications of Type 2 Diabetes$98,000

Funding body: Hunter Medical Research Institute

Funding bodyHunter Medical Research Institute
Project TeamDoctor Rick Thorne, Doctor Lisa Lincz
SchemeProject Grant
RoleLead
Funding Start2010
Funding Finish2010
GNoG0900121
Type Of FundingContract - Aust Non Government
Category3AFC
UONY

Electron Multiplier Charge-Coupled Device Camera. Hamamatsu EM-CCD Camera C9100-13$29,079

Funding body: NHMRC (National Health & Medical Research Council)

Funding bodyNHMRC (National Health & Medical Research Council)
Project TeamConjoint Professor Keith Jones, Professor Eileen McLaughlin, Professor Dirk Van Helden, Doctor Rick Thorne
SchemeEquipment Grant
RoleInvestigator
Funding Start2010
Funding Finish2010
GNoG1000052
Type Of FundingOther Public Sector - Commonwealth
Category2OPC
UONY

AACR 101st Annual Meeting, Washington DC, 17 - 21st April 2010$2,000

Funding body: University of Newcastle - Faculty of Health and Medicine

Funding bodyUniversity of Newcastle - Faculty of Health and Medicine
Project TeamDoctor Rick Thorne
SchemeTravel Grant
RoleLead
Funding Start2010
Funding Finish2010
GNoG1000133
Type Of FundingInternal
CategoryINTE
UONY

20097 grants / $1,366,850

Targeting adaptive mechanisms to endoplasmic reticulum stress in melanoma$491,250

Funding body: NHMRC (National Health & Medical Research Council)

Funding bodyNHMRC (National Health & Medical Research Council)
Project TeamProfessor Xu Dong Zhang, Conjoint Professor Peter Hersey, Doctor Rick Thorne
SchemeProject Grant
RoleInvestigator
Funding Start2009
Funding Finish2009
GNoG0188902
Type Of FundingAust Competitive - Commonwealth
Category1CS
UONY

Confocal Laser Scanning Microscopy for Live Cell Imaging$275,000

Funding body: ARC (Australian Research Council)

Funding bodyARC (Australian Research Council)
Project TeamConjoint Professor Keith Jones, Professor Eileen McLaughlin, Laureate Professor John Aitken, Conjoint Professor Ray Rose, Emeritus Professor John Patrick, Conjoint Professor Christina Offler, Associate Professor David McCurdy, Emeritus Professor Leonie Ashman, Professor Gordon Burns, Professor Dirk Van Helden, Doctor Nikki Verrills, Associate Professor Brett Nixon, Doctor Shaun Roman, Associate Professor Yong-Ling Ruan, Doctor Rick Thorne, Professor Mike Calford
SchemeLinkage Infrastructure Equipment & Facilities (LIEF)
RoleInvestigator
Funding Start2009
Funding Finish2009
GNoG0189038
Type Of FundingScheme excluded from IGS
CategoryEXCL
UONY

FACSAria$30,000

Funding body: NHMRC (National Health & Medical Research Council)

Funding bodyNHMRC (National Health & Medical Research Council)
Project TeamDoctor Rick Thorne, Doctor Severine Roselli, Professor Xu Dong Zhang, Dr Charles De Bock, Conjoint Professor Peter Hersey, Professor Gordon Burns
SchemeEquipment Grant
RoleLead
Funding Start2009
Funding Finish2009
GNoG0189846
Type Of FundingOther Public Sector - Commonwealth
Category2OPC
UONY

Identification of leukaemic stem cells based upon their expression of a novel surface marker, Fat1$25,000

Funding body: Hunter Medical Research Institute

Funding bodyHunter Medical Research Institute
Project TeamDoctor Rick Thorne, Doctor Lisa Lincz
SchemeProject Grant
RoleLead
Funding Start2009
Funding Finish2009
GNoG0189791
Type Of FundingContract - Aust Non Government
Category3AFC
UONY

Hunter Cell Biology Meeting, Pokolbin, 24-27 March 2009$600

Funding body: University of Newcastle - Faculty of Health and Medicine

Funding bodyUniversity of Newcastle - Faculty of Health and Medicine
Project TeamDoctor Rick Thorne
SchemeTravel Grant
RoleLead
Funding Start2009
Funding Finish2009
GNoG0190131
Type Of FundingInternal
CategoryINTE
UONY

20084 grants / $640,125

Involvement of the MIF-CD74-CD44 signalling complex in melanoma progression$600,000

Funding body: Cancer Institute NSW

Funding bodyCancer Institute NSW
Project TeamDoctor Rick Thorne
SchemeCareer Development Fellowship
RoleLead
Funding Start2008
Funding Finish2008
GNoG0188173
Type Of FundingGrant - Aust Non Government
Category3AFG
UONY

Colibri high-performance LED illumination system for fluorescence live cell microscopy$23,225

Funding body: NHMRC (National Health & Medical Research Council)

Funding bodyNHMRC (National Health & Medical Research Council)
Project TeamDoctor Rick Thorne, Dr Charles De Bock, Professor Xu Dong Zhang, Doctor Lisa Lincz, Professor Gordon Burns, Conjoint Professor Peter Hersey, Professor Dirk Van Helden, Conjoint Professor Keith Jones, Professor Roger Smith
SchemeEquipment Grant
RoleLead
Funding Start2008
Funding Finish2008
GNoG0188545
Type Of FundingOther Public Sector - Commonwealth
Category2OPC
UONY

Biogenesis of circulating plasma microparticles in the metabolic syndrome$8,450

Funding body: University of Newcastle

Funding bodyUniversity of Newcastle
Project TeamDoctor Rick Thorne, Doctor Lisa Lincz
SchemePilot Grant
RoleLead
Funding Start2008
Funding Finish2008
GNoG0189072
Type Of FundingInternal
CategoryINTE
UONY

Biogenesis of circulating plasma microparticles in the metabolic syndrome$8,450

Funding body: University of Newcastle - Faculty of Health and Medicine

Funding bodyUniversity of Newcastle - Faculty of Health and Medicine
Project TeamDoctor Rick Thorne, Doctor Lisa Lincz
SchemePilot Grant
RoleLead
Funding Start2008
Funding Finish2008
GNoG0189385
Type Of FundingInternal
CategoryINTE
UONY

20075 grants / $966,500

Characterisation and antibody - mediated targeting of a novel specific marker for T cell all/lymphoblastic lymphoma$564,500

Funding body: NHMRC (National Health & Medical Research Council)

Funding bodyNHMRC (National Health & Medical Research Council)
Project TeamProfessor Gordon Burns, Professor Andrew Boyd, Doctor Rick Thorne, Assoc. Prof Alpha Yap
SchemeProject Grant
RoleInvestigator
Funding Start2007
Funding Finish2007
GNoG0186374
Type Of FundingAust Competitive - Commonwealth
Category1CS
UONY

FACSAria - Fluorescence activated cell sorter$300,000

Funding body: Cancer Institute NSW

Funding bodyCancer Institute NSW
Project TeamDoctor Nikki Verrills, Emeritus Professor Leonie Ashman, Laureate Professor John Aitken, Professor Eileen McLaughlin, Professor Alistair Sim, Doctor Rick Thorne, Doctor Jennette Sakoff
SchemeResearch Infrastructure Grants
RoleInvestigator
Funding Start2007
Funding Finish2007
GNoG0187666
Type Of FundingContract - Aust Non Government
Category3AFC
UONY

HMRI Contribution toward FACSAria - Fluorescence activated cell sorter$52,000

Funding body: Hunter Medical Research Institute

Funding bodyHunter Medical Research Institute
Project TeamDoctor Nikki Verrills, Emeritus Professor Leonie Ashman, Laureate Professor John Aitken, Professor Eileen McLaughlin, Professor Alistair Sim, Doctor Rick Thorne, Doctor Jennette Sakoff
SchemeEquipment Grant
RoleInvestigator
Funding Start2007
Funding Finish2007
GNoG0188207
Type Of FundingOther Public Sector - State
Category2OPS
UONY

High speed/sensitivity CCD camera$30,000

Funding body: NHMRC (National Health & Medical Research Council)

Funding bodyNHMRC (National Health & Medical Research Council)
Project TeamProfessor Dirk Van Helden, Professor Eileen McLaughlin, Professor Gordon Burns, Doctor Rick Thorne, Dr Marcus Howlett, Doctor Mohammad Imtiaz, Professor Alan Brichta, Professor Robert Callister, Doctor Brett Graham, Associate Professor Derek Laver, Associate Professor Liz Milward, Doctor John Holdsworth
SchemeEquipment Grant
RoleInvestigator
Funding Start2007
Funding Finish2007
GNoG0188196
Type Of FundingOther Public Sector - Commonwealth
Category2OPC
UONY

A novel adhesive target involved in the survival of leukaemia cells$20,000

Funding body: Hunter Medical Research Institute

Funding bodyHunter Medical Research Institute
Project TeamDoctor Rick Thorne, Doctor Lisa Lincz
SchemeProject Grant
RoleLead
Funding Start2007
Funding Finish2007
GNoG0187243
Type Of FundingContract - Aust Non Government
Category3AFC
UONY

20062 grants / $152,100

Live cell imaging facility$149,100

Funding body: Cancer Institute NSW

Funding bodyCancer Institute NSW
Project TeamDoctor Rick Thorne, Doctor Nikki Verrills, Professor Alistair Sim, Professor Gordon Burns
SchemeResearch Infrastructure Grants
RoleLead
Funding Start2006
Funding Finish2006
GNoG0186146
Type Of FundingContract - Aust Non Government
Category3AFC
UONY

High speed computer for a live cell imaging system$3,000

Funding body: Hunter Medical Research Institute

Funding bodyHunter Medical Research Institute
Project TeamDoctor Rick Thorne, Professor Gordon Burns, Doctor Nikki Verrills, Professor Alistair Sim
SchemeEquipment Grant
RoleLead
Funding Start2006
Funding Finish2006
GNoG0187279
Type Of FundingNot Known
CategoryUNKN
UONY

20052 grants / $40,000

2005 RIBG allocation$32,000

Funding body: University of Newcastle

Funding bodyUniversity of Newcastle
Project TeamDoctor Rick Thorne
SchemeResearch Infrastructure Block Grant (RIBG)
RoleLead
Funding Start2005
Funding Finish2005
GNoG0185802
Type Of FundingInternal
CategoryINTE
UONY

Understanding how some cancer cells survive low oxygen tension$8,000

Funding body: University of Newcastle

Funding bodyUniversity of Newcastle
Project TeamDoctor Rick Thorne
SchemeProject Grant
RoleLead
Funding Start2005
Funding Finish2005
GNoG0184753
Type Of FundingInternal
CategoryINTE
UONY

20041 grants / $660,000

Investigation of a phagocytic-synapse in the uptake of apoptotic cells$660,000

Funding body: ARC (Australian Research Council)

Funding bodyARC (Australian Research Council)
Project TeamProfessor Gordon Burns, Professor M Berndt, Professor Andrew Boyd, Professor P Hogg, Doctor Rick Thorne
SchemeDiscovery Projects
RoleInvestigator
Funding Start2004
Funding Finish2004
GNoG0182803
Type Of FundingAust Competitive - Commonwealth
Category1CS
UONY

20011 grants / $9,000

Analysis of a Novel Membrane Insertion Motif in the CD36 Glycoprotein$9,000

Funding body: University of Newcastle

Funding bodyUniversity of Newcastle
Project TeamDoctor Rick Thorne
SchemeNew Staff Grant
RoleLead
Funding Start2001
Funding Finish2001
GNoG0180732
Type Of FundingInternal
CategoryINTE
UONY
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Research Supervision

Current Supervision

CommencedResearch Title / Program / Supervisor Type
2011Role of FAT1 Cadherin in Neuronal Differentiation
Medical Science, Faculty of Health and Medicine
Principal Supervisor
2007A novel adhesive target involved in acute lympohoblastic leukemia
Biochemistry & Cell Biology, University of Newcastle
Co-Supervisor
2007Role of Fat1 cadherin in melanoma progression
Biochemistry & Cell Biology, University of Newcastle
Principal Supervisor

Past Supervision

YearResearch Title / Program / Supervisor Type
2014Analysis of Fat1 Cadherin and Identification of Novel Biomarkers for Acute Leukemia
Medical Science, Faculty of Health and Medicine
Principal Supervisor
2014Analysis of Post-Translational Modifications of Fat1 Cadherin
Medical Science, Faculty of Health and Medicine
Principal Supervisor
2013The Role of MIF in Melanoma Progression
Medical Science, Faculty of Health and Medicine
Principal Supervisor
2013Biogenesis of Plasma CD36+Microparticles in Human Diabetes and the Metabolic Syndrome
Pharmacy, Faculty of Health and Medicine
Principal Supervisor
2008Characterisation of the Multifunctional Protein, CREAP
General Medicine, Faculty of Health and Medicine
Co-Supervisor
2005Role of Fat1 cadherin in breast cancer metastasis
Biochemistry & Cell Biology, University of Newcastle
Principal Supervisor
2002TBA
Medical Science, Unknown
Co-Supervisor
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News

brain cancer biobank

Brain cancer biobank

October 29, 2014

A new "biobank" for brain cancer is being established at the Hunter Medical Research Institute courtesy of funding from the Mark Hughes Foundation (MHF).

Molecule may hold key to melanoma progression

Molecule may hold key to melanoma progression

February 27, 2013

The search for new pathways to treat melanoma has unearthed a molecular target that may play an important activation role in tumour growth, according to University of Newcastle researchers.

Dr Rick Thorne

Positions

HCRA Resource Manager
School of Biomedical Sciences and Pharmacy
Faculty of Health and Medicine

Conjoint Senior Lecturer
School of Environmental and Life Sciences
Faculty of Science and Information Technology

Focus area

Medical Biochemistry

Contact Details

Emailrick.thorne@newcastle.edu.au
Phone(02) 4349 4926
Fax(02) 4042 0030

Office

RoomW3-025
BuildingHMRI - Level 3 West
LocationJHH site

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