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Career Summary

Biography

Dr Holt completed her PhD at the University of Newcastle 2002-2006 investigating gene expression in the developing mammalian ovary. Post-doctoral research areas have included nuclear signalling in the developing mammalian testis and currently, control of meiosis and molecular mechanisms underlying aneuploidy in the mammalian female gamete.

Qualifications

  • PhD, University of Newcastle, 17/11/2006
  • Bachelor of Science (Biotechnology)(Honours), University of Newcastle, 30/11/2001

Research

Research keywords

  • Reproductive Medicine
  • meiosis
  • oocytes

Research expertise

  • Egg quality and the ageing ovary
  • Meiosis regulation in the male and female gamete
  • Molecular mechanisms leading to aneuploidy in the egg

Fields of Research

CodeDescriptionPercentage
060100Biochemistry And Cell Biology50
111400Paediatrics And Reproductive Medicine50
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Publications

For publications that are currently unpublished or in-press, details are shown in italics.

Click on a category title below to expand the list of citations for that specific category.

Chapter (2 outputs)

YearCitationAltmetricsLink
2013Holt JE, Lane SIR, Jones KT, 'The Control of Meiotic Maturation in Mammalian Oocytes', , ELSEVIER ACADEMIC PRESS INC 207-226 (2013)
2013Jones KT, Lane SIR, Holt JE, 'Start and Stop Signals of Oocyte Meiotic Maturation', Oogenesis (2013)

Journal article (16 outputs)

YearCitationAltmetricsLink
2014Sadeqzadeh E, de Bock CE, Wojtalewicz N, Holt JE, Smith ND, Dun MD, et al., 'Furin processing dictates ectodomain shedding of human FAT1 cadherin.', Exp Cell Res, 323 41-55 (2014)
2014Holt JE, Pye V, Boon E, Stewart JL, GarcĂ­a-Higuera I, Moreno S, et al., 'The APC/C activator FZR1 is essential for meiotic prophase I in mice.', Development, 141 1354-1365 (2014)
2014Sadeqzadeh E, De Bock CE, Holt JE, Dun MD, Thorne RF, Wojtalewicz N, et al., 'Furin processing dictates ectodomain shedding of human FAT1 cadherin', Experimental Cell Research, 323 41-55 (2014)
2013Merriman JA, Lane SIR, Holt JE, Jennings PC, Garcia-Higuera I, Moreno S, et al., ' Reduced Chromosome Cohesion Measured by Interkinetochore Distance Is Associated with Aneuploidy Even in Oocytes from Young Mice', Biology of Reproduction, 88 31-31 (2013) [C1]
2013Holt JE, Lane SIR, Jones KT, 'Time-lapse epifluorescence imaging of expressed cRNA to cyclin B1 for studying meiosis i in mouse oocytes', Methods in Molecular Biology, 957 91-106 (2013)
2012Holt JE, Lane SI, Jennings PC, Garcia-Higuera I, Moreno S, Jones KT, 'APC FZR1 prevents nondisjunction in mouse oocytes by controlling meiotic spindle assembly timing', Molecular Biology of the Cell, 23 3970-3981 (2012) [C1]

Co-authors: Keith Jones

2012Seah KYM, Holt JE, Garcia-Higuera I, Moreno S, Jones KT, 'The APC activator fizzy-related-1 (FZR1) is needed for preimplantation mouse embryo development', Journal of Cell Science, 125 6030-6037 (2012) [C1]

Co-authors: Keith Jones

2011Holt JE, Tran SM-T, Stewart JL, Minahan KL, Garcia-Higuera I, Moreno S, Jones KT, 'The APC/C activator FZR1 coordinates the timing of meiotic resumption during prophase I arrest in mammalian oocytes', Development, 138 905-913 (2011) [C1]
2010Holt JE, Stewart JL, Jones KT, 'Spatial regulation of APC(Cdh1)-induced cyclin B1 degradation maintains G2 arrest in mouse oocytes', Development, 137 1297-1304 (2010) [C1]
2010Jones KT, Holt JE, 'BubR1 highlights essential function of Cdh1 in mammalian oocytes', Cell Cycle, 9 1029-1030 (2010) [C3]

Co-authors: Keith Jones

2009Holt JE, Jones KT, 'Control of homologous chromosome division in the mammalian oocyte', Molecular Human Reproduction, 15 139-147 (2009) [C1]
2007Holt JE, Jans DA, Ly-Huynh JD, Efthymiadis A, Hime GR, Loveland KL, Jans DA, 'Regulation of Nuclear Import During Differentiation: The IMP alpha gene family and spermatogenesis', Current Genomics, 8 323-334 (2007) [C1]
2006Holt JE, Roman SD, Aitken RJ, McLaughlin EA, 'Identification and characterization of a novel Mt-retrotransposon highly represented in the female mouse germline', Genomics, 87 490-499 (2006) [C1]
2006Curry BJ, Holt JE, McLaughlin EA, Aitken RJ, 'Characterization of structure and expression of the Dzip1 gene in the rat and mouse', Genomics, 87 275-285 (2006) [C1]
2006Holt JE, Jackson A, Roman SD, Aitken RJ, Koopman PA, McLaughlin EA, 'CXCR4/SDF1 interaction inhibits the primordial to primary follicle transition in the neonatal mouse ovary', Developmental Biology, 293 449-460 (2006) [C1]
2004Blackmore DG, Baillie LR, Holt JE, Dierkx LM, Aitken RJ, McLaughlin EA, 'Biosynthesis of the Canine Zona Pellucida Requires the Integrated Participation of Both Oocytes and Granulosa Cells', Biology of Reproduction, 71 661-668 (2004) [C1]
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Conference (8 outputs)

YearCitationAltmetricsLink
2010Hopkins LA, Pye VJ, Fraser BA, Holt JE, Jones KT, McLaughlin EA, 'The Role of Fizzy Related 1 in Male Meiosis', Reproduction, Fertility and Development, Sydney (2010) [E3]
2009Jones KT, Weaver J, Holt JE, 'The mechanism by which fizzy-related (Fzr1) controls pro-phase 1 arrest in mouse oocytes', Biology of Reproduction, Pittsburgh, PA (2009) [E3]

Co-authors: Keith Jones

2007Gunter KM, Fraser BA, Holt JE, Stanger SJ, Aitken RJ, Roman SD, McLaughlin EA, 'CXCR4/SDF1 interaction in the neonatal mouse testes', 7th Hunter Cellular Biology Meeting. Program, Hunter Valley, NSW (2007) [E3]
2006Fraser BA, Holt JE, Andrew J, Roman SD, Stanger SJ, Aitken RJ, et al., 'CXCR4/SDF1 Interactionin the Embryonic and Neonatal Mpise Testes', Abstracts, Brisbane Convention Centre, QLD, Australia (2006) [E3]
2006Holt JE, Andrew J, Roman SD, Stanger SJ, Aitken RJ, Peter K, McLaughlin EA, 'CXCR4/SDF-1 interaction in the embryonic and neonatal mouse tests', Book of Abstracts, Chicago, Illinois (2006) [E3]
2005Holt JE, Aitken RJ, Roman SD, McLaughlin E, 'Use of RNA interference for the functional analysis of genes involved in ovarian development', MECHANISMS OF DEVELOPMENT (2005) [E3]
2004Holt JE, Aitken RJ, Roman SD, McLaughlin EA, 'Expression of the Chemokine CXCL12 and its receptor CXCR4 in the activating Mammalian Ovarian follicle', Reproduction, Fertility and Development, Sydney (2004) [E3]
2003Holt JE, Aitken RJ, Roman SD, McLaughlin EA, 'Gene expression in the activating mammalian ovarian follicle', ComBio Combined Conference Abstracts, Melbourne (2003) [E3]
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Grants and Funding

Summary

Number of grants12
Total funding$1,319,020

- Indicates that the researcher may be seeking students for this project.

Click on a grant title below to expand the full details for that specific grant.

2014 (2 grants)

miRNA regulation of sperm maturation $383,447
Funding Body: NHMRC (National Health & Medical Research Council)

Project Team
Associate Professor Brett Nixon, Professor Eileen McLaughlin, Doctor Janet Holt
SchemeRole
Project GrantInvestigator
Total AmountFunding StartFunding Finish
$383,44720142016
GNo:G1300125

The Role of the GTPase Dynamin in the Female Germline$30,000
Funding Body: Hunter Medical Research Institute

Project Team
Ms Kate Redgrove, Doctor Janet Holt, Professor Eileen McLaughlin, Associate Professor Brett Nixon
SchemeRole
Bridging GrantInvestigator
Total AmountFunding StartFunding Finish
$30,00020142014
GNo:G1301333

2013 (2 grants)

Leica TP 1020 Automatic Tissue Processor for histology applications$35,000
Funding Body: NHMRC (National Health & Medical Research Council)

The Annual Scientific Meeting of the Endocrine Society of Australia and Society of Reproductive Biology, Sydney 25-28 August 2013.$595
Funding Body: University of Newcastle - Faculty of Health

Project Team
Doctor Janet Holt
SchemeRole
Travel GrantChief Investigator
Total AmountFunding StartFunding Finish
$59520132013
GNo:G1300895

2012 (2 grants)

Regulation of germ cell number and quality by Fizzy-Related protein$375,000
Funding Body: ARC (Australian Research Council)

Project Team
Doctor Janet Holt
SchemeRole
Discovery Early Career Researcher Award (DECRA)Chief Investigator
Total AmountFunding StartFunding Finish
$375,00020122014
GNo:G1100556

The control of chromosome division during female meiosis$330,000
Funding Body: ARC (Australian Research Council)

Project Team
Doctor Janet Holt
SchemeRole
Discovery ProjectsChief Investigator
Total AmountFunding StartFunding Finish
$330,00020122014
GNo:G1100078

2011 (5 grants)

2010 Research Fellowship - PRCRS (DECRA 1/3/2012)$121,978
Funding Body: University of Newcastle

Project Team
Doctor Janet Holt
SchemeRole
Research FellowshipChief Investigator
Total AmountFunding StartFunding Finish
$121,97820112013
GNo:G1100163

Eppendorf mastercycler pro with thermomixer comfort and 5430R centrifuge$15,000
Funding Body: NHMRC (National Health & Medical Research Council)

Project Team
Professor Eileen McLaughlin, Professor Peter Lewis, Professor Adam McCluskey, Conjoint Professor Keith Jones, Associate Professor Brett Nixon, Doctor Shaun Roman, Doctor Jennette Sakoff, Doctor Ian Grainge, Doctor Janet Holt, Doctor Xiao Yang
SchemeRole
Equipment GrantInvestigator
Total AmountFunding StartFunding Finish
$15,00020112011
GNo:G1100028

Mechanisms underlying the incidence of aneuploidy in mammalian eggs$15,000
Funding Body: University of Newcastle

Project Team
Doctor Janet Holt
SchemeRole
Fellowship GrantChief Investigator
Total AmountFunding StartFunding Finish
$15,00020112011
GNo:G1100395

IMPLEN NanoPhotometer pearl$10,000
Funding Body: NHMRC (National Health & Medical Research Council)

Project Team
Conjoint Associate Professor Murray Cairns, Associate Professor Paul Tooney, Associate Professor Alan Brichta, Emeritus Professor John Rostas, Emeritus Professor Patricia Michie, Conjoint Professor Keith Jones, Professor Ulli Schall, Associate Professor Phillip Dickson, Doctor Frederick Walker, Doctor Rick Thorne, Doctor Chris Dayas, Doctor Nikki Verrills, Doctor Janet Holt, Doctor Severine Roselli, Doctor Kathryn Skelding, Doctor Jude Weidenhofer, Associate Professor Liz Milward, Doctor Charles De Bock, Doctor Julie Merriman-Jones, Doctor Jing Qin Wu, Doctor Bing Liu, Mr Dan Johnstone, Ms Belinda Goldie, Ms Natalie Beveridge
SchemeRole
Equipment GrantInvestigator
Total AmountFunding StartFunding Finish
$10,00020112011
GNo:G1100030

EMBO Meiosis Meeting, Hotel Ariston and Congress Centre, Cappacio, Italy, 17 - 21 September 2011$1,500
Funding Body: University of Newcastle - Faculty of Health

Project Team
Doctor Janet Holt
SchemeRole
Travel GrantChief Investigator
Total AmountFunding StartFunding Finish
$1,50020112011
GNo:G1100939

2010 (1 grants)

Cold Springs Harbor Germ Cell Conference, Cold Spring Harbor Laboratory, NT, USA, 5 - 9 October 2010$1,500
Funding Body: University of Newcastle - Faculty of Health

Project Team
Doctor Janet Holt
SchemeRole
Travel GrantChief Investigator
Total AmountFunding StartFunding Finish
$1,50020102010
GNo:G1000768
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Research Supervision

Number of current supervisions4
Total current UoN PhD EFTSL1.3

For supervisions undertaken at an institution other that the University of Newcastle, the institution name is listed below the program name.

Current Supervision

CommencedProposed
Completion
ProgramSupervisor TypeResearch Title
20142018PhD (Medical Genetics)Co-SupervisorTo Investigate the Interaction of Breast Cancer and Ovarian Cancer
20132017PhD (Biological Sciences)Principal SupervisorThe Impact of Maternal Ageing and Chemotoxicants on Female Fertility
20132017PhD (Medical Biochemistry)Co-SupervisorRole of Stromal WNT Signaling in Ovarian Epithelial Carcinogenesis
20102014PhD (Human Physiology)Principal SupervisorElucidation of Higher Meiotic Errors on Oocyte from Advanced Maternat Ages through Kinetochore Tracking

Past Supervision

YearProgramSupervisor TypeResearch Title
2013PhD (Human Physiology)Co-SupervisorRole of Fzr1 in Embryogenesis
2012M Philosophy (Human Physiolog)Co-SupervisorCharacterisation of an Oocyte Specific Knockout Model of Cdh1
2012PhD (Human Physiology)Co-SupervisorControl of Chromosome Segregation in Mouse Oocytes
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Dr Janet Holt

Work Phone(02) 49854484
Email
PositionARC DECRA FELLOW
School of Biomedical Sciences and Pharmacy
Faculty of Health and Medicine
The University of Newcastle, Australia
Focus AreaHuman Physiology
Office
LS2.39,
Life Sciences Building,
Callaghan
University Drive
Callaghan NSW 2308
Australia
URL:www.newcastle.edu.au/profile/janet-holt