Dr Gemma Madsen

Senior Research Officer

School of Medicine and Public Health (Reproductive Medicine)

Career Summary

Biography

Research Expertise

Reproductive Medicine, Protein Biochemistry, Tissue Culture, Immunocytochemistry, Immunology, Psychoimmunology, Virology, Molecular Biology, Conference Organisation, Data Management and Co-ordinator of Multicentre Clinical Trial.


Qualifications

  • PhD (Medicine), University of Newcastle

Keywords

  • Biochemistry, Molecular Biology
  • Reproductive medicine, preterm birth, pregnancy

Languages

  • English (Fluent)

Fields of Research

CodeDescriptionPercentage
110999Neurosciences not elsewhere classified35
111499Paediatrics and Reproductive Medicine not elsewhere classified65

Professional Experience

UON Appointment

DatesTitleOrganisation / Department
23/01/2015 - 31/12/2015Senior Research OfficerUniversity of Newcastle
School of Medicine and Public Health
Australia
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Publications

For publications that are currently unpublished or in-press, details are shown in italics.


Chapter (1 outputs)

YearCitationAltmetricsLink
2000Smith R, Changs E-C, Mesiano S, Nicholson R, Cheng Y-H, Bowman M, et al., 'Regulation of parturition: The role of CRH', Hormones and Women's Health The Reproductive Years, Harwood Academic Publishers, Amsterdam, The Netherlands 215-219 (2000) [B1]
Co-authorsVicki Clifton, John Fitter, Maria Bowman, Roger Smith

Journal article (24 outputs)

YearCitationAltmetricsLink
2014Chai SY, Smith R, Fitter JT, Mitchell C, Pan X, Ilicic M, et al., 'Increased progesterone receptor a expression in labouring human myometrium is associated with decreased promoter occupancy by the histone demethylase JARID1A', Molecular Human Reproduction, 20 442-453 (2014) [C1]

Progesterone regulates female reproductive function predominantly through two nuclear progesterone receptors (PRs), PR-A and PR-B. During human parturition myometrial PR expressio... [more]

Progesterone regulates female reproductive function predominantly through two nuclear progesterone receptors (PRs), PR-A and PR-B. During human parturition myometrial PR expression is altered to favour PR-A, which activates pro-labour genes. We have previously identified histone H3 lysine 4 trimethylation (H3K4me3) as an activator of myometrial PR-A expression at labour. To further elucidate the mechanisms regulating PR isoform expression in the human uterus at labour, we have (i) determined the methylation profile of the cytosine-guanine dinucleotides (CpG) island in the promoter region of the PR gene and (ii) identified the histone-modifying enzymes that target the H3K4me3 mark at the PR promoters in term and preterm human myometrial tissues obtained before and after labour onset. Bisulphite sequencing showed that despite overall low levels of PR CpG island methylation, there was a significant decrease in methylated CpGs with labour in both preterm (P < 0.05) and term (P < 0.01) groups downstream of the PR-B transcription start site. This methylation change was not associated with altered PR-B expression, but may contribute to the increase in PR-A expression with labour. Chromatin immunoprecipitation revealed that the histone methyltransferase, SET and MYND domain-containing protein 3 (SMYD3), bound to the PR gene at significantly higher levels at the PR-A promoter compared with the PR-B promoter (P < 0.010), with no labour-associated changes observed. The H3K4 demethylase, Jumonji AT-rich interactive domain 1A (JARID1A), also bound to the PR-A, but not to the PR-B promoter prior to term labour, and decreased significantly at the onset of labour (P = 0.014), providing a mechanism for the previously reported increase in H3K4me3 level and PR-A expression with labour. Our studies suggest that epigenetic changes mediated by JARID1A, SMYD3 and DNA methylation may be responsible, at least in part, for the functional progesterone withdrawal that precipitates human labour. © The Author 2014. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved.

DOI10.1093/molehr/gau005
CitationsScopus - 2Web of Science - 2
Co-authorsJohn Fitter, Roger Smith
2013Smith R, Zakar T, Madsen G, 'Mammalian Labor: Variations on a Theme by Amniota', ENDOCRINOLOGY, 154 584-588 (2013) [C1]
DOI10.1210/en.2012-2262Author URL
Co-authorsRoger Smith
2012Smith R, Paul JW, Maiti K, Tolosa Gonzalez JM, Madsen GM, 'Recent advances in understanding the endocrinology of human birth', Trends in Endocrinology & Metabolism, 23 516-523 (2012) [C1]
CitationsScopus - 6Web of Science - 5
Co-authorsJonathan Paul, Roger Smith
2012Chai S, Smith R, Zakar T, Mitchell CM, Madsen GM, 'Term myometrium is characterized by increased activating epigenetic modifications at the progesterone receptor-A promoter', Molecular Human Reproduction, 18 401-409 (2012) [C1]
CitationsScopus - 8Web of Science - 5
Co-authorsRoger Smith
2009Merlino A, Welsh T, Erdonmez T, Madsen GM, Zakar T, Smith R, et al., 'Nuclear progesterone receptor expression in the human fetal membranes and decidua at term before and after labor', Reproductive Sciences, 16 357-363 (2009) [C1]
DOI10.1177/1933719108328616
CitationsScopus - 18Web of Science - 17
Co-authorsRoger Smith
2007Madsen GM, Macintyre DA, Mesiano S, Smith R, 'Progesterone receptor or cytoskeletal protein?', Reproductive Sciences, 14 217-222 (2007) [C1]
DOI10.1177/1933719107302380
CitationsScopus - 9Web of Science - 8
Co-authorsRoger Smith
2006Gill AW, Madsen GM, Knox M, Bisits AM, Giles WB, Tudehope D, et al., 'Neonatal neurodevelopmental outcomes following tocolysis with glycerol trinitrate patches', American Journal of Obstetrics and Gynecology, 195 484-487 (2006) [C1]
DOI10.1016/j.ajog.2006.01.103
CitationsScopus - 4Web of Science - 6
Co-authorsRoger Smith
2004Madsen GM, Zakar T, Ku CY, Sanborn B, Smith R, Mesiano S, 'Prostaglandins Differentially Modulate Progesterone Receptor-A and -B Expression in Human Myometrial Cells: Evidence for Prostaglandin-Induced Functional Progesterone Withdrawal', Endocrinology and Metabolism, 89 1010-1013 (2004) [C1]
DOI10.1210/jc.2003-031037
CitationsScopus - 66Web of Science - 55
Co-authorsRoger Smith
2004Bisits AM, Madsen GM, Knox M, Gill AW, Smith R, Yeo GS, et al., 'The Randomized Nitric Oxide Tocolysis Trial (RNOTT) for the treatment of preterm labor', American Journal of Obstetrics and Gynecology, 191 683-690 (2004) [C1]
DOI10.1016/j.ajog.2004.02.019
CitationsScopus - 32Web of Science - 22
Co-authorsRoger Smith
2004Madsen GM, Zakar T, Manuelpillai U, Wallace E, Kwek K, Yeo GS, et al., 'Intracrine Control of Estrogen Action in Human Gestational Tissues at Parturition', Society for Gynecologic Investigation. Journal, 11 213-219 (2004) [C1]
DOI10.1016/j.jsgi.2003.12.003
CitationsScopus - 5Web of Science - 5
Co-authorsRoger Smith
2001Leung T, Chung T, Madsen GM, Lam P, Sahota D, Smith R, 'Rate of rise in maternal plasma corticotrohpin-releasing hormone and its relation to gestational length', BJOG: An international Journal of Obstetrics and Gynaecology, 108 527-532 (2001) [C1]
CitationsScopus - 20Web of Science - 15
Co-authorsRoger Smith
2000Clifton VL, Qing G, Murphy VE, Schwartz J, Madsen GM, Smith R, 'Localization and Characterization of Urocortin during Human Pregancy', Placenta, 21 782-788 (2000) [C1]
CitationsScopus - 44Web of Science - 39
Co-authorsRoger Smith, Vicki Clifton, Vanessa Murphy
2000Leung TN, Chung T, Madsen GM, Lam C, Lam P, Walters W, Smith R, 'Analysis of mid-trimester corticotrophin-releasing hormone and a-fetoprotein concentrations for predicting pre-eclampsia', Human Reproduction, 15 1813-1818 (2000) [C1]
CitationsScopus - 21Web of Science - 19
Co-authorsRoger Smith
2000Giles W, Bisits A, Knox M, Madsen GM, Smith R, 'The effect of fetal fibronectin testing on admissions to a tertiary maternal-fetal medicine unit and cost savings', American Journal of Obstetrics and Gynecology, 182 439-442 (2000) [C1]
Co-authorsRoger Smith
1999Smith R, Wicking J, Bowman M, Belleoud A, Dubreuil G, Davies J, Madsen GM, 'Corticotropin-releasing hormone in chimpazee and gorilla pregnancies', Journal of clinical endocrinology and Metabolism, (Aug);84/8 2820-2825 (1999) [C1]
CitationsScopus - 52Web of Science - 44
Co-authorsMaria Bowman, Roger Smith
1999Leung TN, Chung T, Madsen GM, McLean M, Chang AMZ, Smith R, 'Elevated mid-trimester maternal coritcotropin-releasing hormone levels in pregnancies destined to deliver preterm before 34 weeks', British Jounal of Obsetrics and Gynecology, (Oct);106 1041-1046 (1999) [C1]
CitationsScopus - 59Web of Science - 47
Co-authorsRoger Smith
1998Bisits AM, Madsen GM, McLean MT, Ocallaghan S, Smith R, Giles WB, 'CRH- a biomechnaical predictor of preterm delivery in a pilot randomised trial - of the treatment of preterm labour', American Journal of Obstetrics & Gynecology, 178 862-866 (1998) [C1]
Co-authorsRoger Smith
1998Bisits A, Madsen GM, McLean M, Ocallaghan S, Smith R, Giles W, 'Corticotropin-releasing hormone:a biochemical predictor of preterm delivery in a pilot randomised trial of the treatment of preterm labour', American Journal of Obstetrics and Gynaecology, 178(4) 862 (1998) [C1]
CitationsScopus - 30Web of Science - 27
Co-authorsRoger Smith
1998Batey RG, Madsen GM, 'The carbohydrate-deficient transferrin test in hospital practice', Drug and Alcohol Review, 17 105-109 (1998) [C1]
1998Chan EC, Falconer J, Madsen GM, Rice KC, Webster EL, Chrousos GP, Smith R, 'Corticotropin-releasing hormone type 1 receptor antagonist delays parturition in sheep', Endocrinology, 139(7) 3357-3360 (1998) [C1]
CitationsScopus - 55Web of Science - 46
Co-authorsRoger Smith
1988POWIS DA, MADSEN GM, TOROK TL, 'AUGMENTATION OF THE INDIRECT SYMPATHOMIMETIC ACTION OF TYRAMINE BY CARDIOACTIVE STEROIDS IS A CONSEQUENCE OF ELEVATED INTRACELLULAR SODIUM', NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY, 337 273-278 (1988)
Author URL
CitationsScopus - 2Web of Science - 3
1986Powis DA, Madsen GM, 'Unsuitability of the 86Rb+ uptake method for estimation of (Na+ + K+)-ATPase activity in innervated tissues', BBA - Biomembranes, 861 251-258 (1986)

(Na+ + K+)-ATPase activity was estimated by 86Rb+ uptake in dog saphenous vein to determine the validity of the technique in tissues that have a sympathetic innervation. When saph... [more]

(Na+ + K+)-ATPase activity was estimated by 86Rb+ uptake in dog saphenous vein to determine the validity of the technique in tissues that have a sympathetic innervation. When saphenous vein rings were incubated at 37°C in Krebs' solution containing 86Rb+, the cardenolide acetylstrophanthidin caused a concentration-dependent inhibition of Rb+ uptake. The threshold for inhibition was approx. 10 nM acetylstrophanthidin and the maximum effect was obtained at 9 µM. In the upper part of this concentration range (> 1 µM) acetylstrophanthidin released noradrenaline from the sympathetic nerve terminals associated with the tissue. In this upper part of the acetylstrophanthidin concentration range the a-adrenoceptor antagonist phentolamine (8 µM) reduced, by up to 25%, the degree of 86Rb+ uptake inhibition caused by the cardenolide. In other experiments, saphenous vein strips were loaded with 86Rb+ and perifused with Krebs' solution containing acetylstrophanthidin. At concentrations which release noradrenaline, acetylstrophanthidin increased the efflux of 86Rb+. Phentolamine (8 µM) prevented the acetylstrophanthidin-evoked efflux of the isotope as did prior in vitro denervation of 86Rb+ loaded strips with 6-hydroxydopamine. Exogenous noradrenaline (1-100 µM) added to the perifusing fluid also caused an efflux of 86Rb+ that was attenuated by phentolamine. The data indicate for dog saphenous vein that with low concentrations of acetylstrophanthidin the extent of 86Rb+ accumulation might accurately reflect prevailing (Na+ + K+)-ATPase activity. At higher concentrations of acetylstrophanthidin, however, noradrenaline is released from the nerve endings and causes 86Rb+ efflux from the smooth muscle cells consequent upon a-adrenoceptor activation. Since this efflux reduces the extent of Rb+ accumulation, measurement of the latter does not adequately reflect uptake mediated by the activity of (Na+ + K+)-ATPase. This is significant because in most applications of the 86Rb+ uptake method it is the estimate of Rb+ accumulation made in the presence of a high concentration of cardenolide that forms the basis of all subsequent calculations with respect to (Na+ + K+)- ATPase activity. © 1986.

DOI10.1016/0005-2736(86)90427-X
CitationsScopus - 1
1986POWIS DA, MADSEN GM, 'UNSUITABILITY OF THE RB-86(+) UPTAKE METHOD FOR ESTIMATION OF (NA+ + K+)-ATPASE ACTIVITY IN INNERVATED TISSUES', BIOCHIMICA ET BIOPHYSICA ACTA, 861 251-258 (1986)
DOI10.1016/0005-2736(86)90427-XAuthor URL
CitationsWeb of Science - 4
1986POWIS DA, MADSEN GM, 'ALPHA-ADRENOCEPTOR-MEDIATED INCREASE IN TENSION AND EFFLUX OF RB-86 IN DOG SAPHENOUS-VEIN', BLOOD VESSELS, 23 95-96 (1986)
Author URL
CitationsWeb of Science - 1
Show 21 more journal articles

Conference (7 outputs)

YearCitationAltmetricsLink
2012Chai S, Fitter JT, Smith R, Madsen GM, 'DNA methylation of progesterone receptor isoforms in term human myometrium', Abstracts. The Annual Scientific Meeting of the Endocrine Society of Australia and the Society for Reproductive Biology 2012, Gold Coast, QLD (2012) [E3]
Co-authorsJohn Fitter, Roger Smith
2012Mitchell CM, Chai S, Madsen GM, Hirst JJ, Zakar T, 'Bivalent epigenetic modifications regulate PTGS2 expression during gestation in the amnion', Reproductive Sciences, San Diego, CA (2012) [E3]
Co-authorsJon Hirst
2011Chai SY, Smith R, Zakar T, Mitchell CM, Madsen GM, 'Term myometrium is characterized by increased activating epigenetic marks on the progesterone receptor A promoter', Reproductive Sciences, Miami Beach, Florida (2011) [E3]
Co-authorsRoger Smith
2010Chai S, Smith R, Madsen GM, 'Epigenetic regulation of progesterone receptor isoform expression in term human myometrium', The Endocrine Society of Australia Annual Scientific Meeting Proceedings and Abstract Book, Sydney (2010) [E3]
Co-authorsRoger Smith
2004Madsen GM, Zakar T, Chan EC, Yeo GS, Kwek K, Mesiano S, 'Prostaglandin Receptor Expression in Human Myometrium Before and After the Onset of Term Labor', Prostaglandin Receptor Expression in Human Myometrium Before and After the Onset of Term Labor, Texas (2004) [E3]
2001Giles WB, Knox M, Madsen GM, Bisits AM, Gill AW, Smith R, et al., 'The randomised nitric oxide tocolysis trial', American Journal of Obstetrics and Gynecology, Reno, Nevada (2001) [E3]
1998Giles WB, Knox M, Masden G, Bisits AM, Smith R, 'The effect of fetal fibronectin usage on admissions to a tertiary maternal fetal medicine unit and cost saving', American Journal of Obstetrics and Gynecology, Miami, 1998 (1998) [E1]
CitationsScopus - 41Web of Science - 33
Co-authorsRoger Smith
Show 4 more conferences
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Grants and Funding

Summary

Number of grants11
Total funding$507,577

Click on a grant title below to expand the full details for that specific grant.


20151 grants / $25,222

Regulation of estrogen activation in human parturition$25,222

Funding body: John Hunter Hospital Charitable Trust Fund

Funding bodyJohn Hunter Hospital Charitable Trust Fund
Project TeamDoctor Gemma Madsen, Dr Sharyn Rayner
SchemeResearch Grant
RoleLead
Funding Start2015
Funding Finish2015
GNoG1500609
Type Of FundingGrant - Aust Non Government
Category3AFG
UONY

20131 grants / $23,810

Epigenetic Regulation of Progesterone Receptors and the Onset of Labour$23,810

Funding body: John Hunter Hospital Charitable Trust Fund

Funding bodyJohn Hunter Hospital Charitable Trust Fund
Project TeamDoctor Gemma Madsen, Dr Sharyn Rayner
SchemeResearch Grant
RoleLead
Funding Start2013
Funding Finish2013
GNoG1300703
Type Of FundingOther Public Sector - State
Category2OPS
UONY

20122 grants / $44,454

The regulation of progesterone withdrawal in human parturition$34,454

Funding body: John Hunter Hospital Charitable Trust Fund

Funding bodyJohn Hunter Hospital Charitable Trust Fund
Project TeamDoctor Gemma Madsen, Dr Sharyn Rayner
SchemeResearch Grant
RoleLead
Funding Start2012
Funding Finish2012
GNoG1200642
Type Of FundingOther Public Sector - State
Category2OPS
UONY

Precellys Tissue Homogeniser$10,000

Funding body: NHMRC (National Health & Medical Research Council)

Funding bodyNHMRC (National Health & Medical Research Council)
Project TeamProfessor Roger Smith, Professor Eugenie Lumbers, Conjoint Professor Tam Zakar, Professor Jon Hirst, Conjoint Professor Ian Wright, Doctor Gemma Madsen, Doctor Kaushik Maiti
SchemeEquipment Grant
RoleInvestigator
Funding Start2012
Funding Finish2012
GNoG1100979
Type Of FundingOther Public Sector - Commonwealth
Category2OPC
UONY

20111 grants / $69,157

Research microscope, confocal ready nikon eclipse 90i microscope$69,157

Funding body: NHMRC (National Health & Medical Research Council)

Funding bodyNHMRC (National Health & Medical Research Council)
Project TeamProfessor Roger Smith, Conjoint Professor Tam Zakar, Professor Jon Hirst, Doctor Kaushik Maiti, Doctor Gemma Madsen, Professor Rodney Scott, Conjoint Professor Peter Wark, Laureate Professor Paul Foster, Professor Phil Hansbro, Conjoint Professor Ian Wright
SchemeEquipment Grant
RoleInvestigator
Funding Start2011
Funding Finish2011
GNoG1100024
Type Of FundingOther Public Sector - Commonwealth
Category2OPC
UONY

20101 grants / $303,750

Regulation of progesterone action in human parturition$303,750

Funding body: NHMRC (National Health & Medical Research Council)

Funding bodyNHMRC (National Health & Medical Research Council)
Project TeamDoctor Gemma Madsen
SchemeProject Grant
RoleLead
Funding Start2010
Funding Finish2010
GNoG0190155
Type Of FundingAust Competitive - Commonwealth
Category1CS
UONY

20091 grants / $18,286

Epigenetic regulation of progesterone receptor isoform expression in human myometrium$18,286

Funding body: John Hunter Hospital Charitable Trust Fund

Funding bodyJohn Hunter Hospital Charitable Trust Fund
Project TeamDoctor Gemma Madsen
SchemeResearch Grant
RoleLead
Funding Start2009
Funding Finish2009
GNoG0190236
Type Of FundingOther Public Sector - State
Category2OPS
UONY

20081 grants / $15,208

Regulation of the differential expression of the A and B isoforms of the progesterone receptor in human parturition$15,208

Funding body: John Hunter Hospital Charitable Trust Fund

Funding bodyJohn Hunter Hospital Charitable Trust Fund
Project TeamDoctor Gemma Madsen, Doctor Fatima Patel
SchemeResearch Grant
RoleLead
Funding Start2008
Funding Finish2008
GNoG0189518
Type Of FundingOther Public Sector - State
Category2OPS
UONY

20051 grants / $1,220

Society of Gynecologic Investigation 1st International Summit on Preterm Birth, 10-12 November 2005$1,220

Funding body: University of Newcastle

Funding bodyUniversity of Newcastle
Project TeamDoctor Gemma Madsen
SchemeTravel Grant
RoleLead
Funding Start2005
Funding Finish2005
GNoG0185914
Type Of FundingInternal
CategoryINTE
UONY

20041 grants / $1,470

Society of Gynecologic Investigation 51st Annual Scientific Meeting, 24 to 27 March 2004$1,470

Funding body: University of Newcastle

Funding bodyUniversity of Newcastle
Project TeamDoctor Gemma Madsen
SchemeTravel Grant
RoleLead
Funding Start2004
Funding Finish2004
GNoG0183892
Type Of FundingInternal
CategoryINTE
UONY

20001 grants / $5,000

Role of estrogen receptors (ERa and ERb) in the regulation of human parturition.$5,000

Funding body: ARC (Australian Research Council)

Funding bodyARC (Australian Research Council)
Project TeamDoctor Sam Mesiano, Doctor Gemma Madsen
SchemeSmall Grant
RoleInvestigator
Funding Start2000
Funding Finish2000
GNoG0178902
Type Of FundingScheme excluded from IGS
CategoryEXCL
UONY
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Research Supervision

Current Supervision

CommencedResearch Title / Program / Supervisor Type
2013Epigenetic Regulation of Progesterone Receptors and the Onset of Labour
Medical Studies, Faculty of Health and Medicine
Co-Supervisor
2009Epigenetic Regulation of Progesterone Receptor Isoforms in Human Paturition
Medical Studies, Faculty of Health and Medicine
Principal Supervisor
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Dr Gemma Madsen

Position

Senior Research Officer
Mothers and Babies Research Centre
School of Medicine and Public Health
Faculty of Health and Medicine

Focus area

Reproductive Medicine

Contact Details

Emailgemma.madsen@newcastle.edu.au
Phone(02) 4042 0359
Fax(02) 4042 0045

Office

Room3403-HMRI (Mothers and Babies Research Centre)
BuildingHMRI - John Hunter Hospital
LocationHMRI-John Hunter Hospital

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