Dr Gemma Madsen

Senior Research Officer

School of Medicine and Public Health (Reproductive Medicine)

Career Summary

Biography

Research Expertise
Reproductive Medicine, Protein Biochemistry, Tissue Culture, Immunocytochemistry, Immunology, Psychoimmunology, Virology, Molecular Biology, Conference Organisation, Data Management and Co-ordinator of Multicentre Clinical Trial.

Qualifications

  • PhD (Medicine), University of Newcastle

Keywords

  • Biochemistry, Molecular Biology
  • Reproductive medicine, preterm birth, pregnancy

Languages

  • English (Fluent)

Fields of Research

Code Description Percentage
110999 Neurosciences not elsewhere classified 35
111499 Paediatrics and Reproductive Medicine not elsewhere classified 65

Professional Experience

UON Appointment

Title Organisation / Department
Senior Research Officer University of Newcastle
School of Medicine and Public Health
Australia
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Publications

For publications that are currently unpublished or in-press, details are shown in italics.


Chapter (1 outputs)

Year Citation Altmetrics Link
2000 Smith R, Changs E-C, Mesiano S, Nicholson R, Cheng Y-H, Bowman M, et al., 'Regulation of parturition: The role of CRH', Hormones and Women's Health The Reproductive Years, Harwood Academic Publishers, Amsterdam, The Netherlands 215-219 (2000) [B1]
Co-authors Vicki Clifton, John Fitter, Maria Bowman, Roger Smith

Journal article (24 outputs)

Year Citation Altmetrics Link
2014 Chai SY, Smith R, Fitter JT, Mitchell C, Pan X, Ilicic M, et al., 'Increased progesterone receptor a expression in labouring human myometrium is associated with decreased promoter occupancy by the histone demethylase JARID1A', Molecular Human Reproduction, 20 442-453 (2014) [C1]

Progesterone regulates female reproductive function predominantly through two nuclear progesterone receptors (PRs), PR-A and PR-B. During human parturition myometrial PR expressio... [more]

Progesterone regulates female reproductive function predominantly through two nuclear progesterone receptors (PRs), PR-A and PR-B. During human parturition myometrial PR expression is altered to favour PR-A, which activates pro-labour genes. We have previously identified histone H3 lysine 4 trimethylation (H3K4me3) as an activator of myometrial PR-A expression at labour. To further elucidate the mechanisms regulating PR isoform expression in the human uterus at labour, we have (i) determined the methylation profile of the cytosine-guanine dinucleotides (CpG) island in the promoter region of the PR gene and (ii) identified the histone-modifying enzymes that target the H3K4me3 mark at the PR promoters in term and preterm human myometrial tissues obtained before and after labour onset. Bisulphite sequencing showed that despite overall low levels of PR CpG island methylation, there was a significant decrease in methylated CpGs with labour in both preterm (P < 0.05) and term (P < 0.01) groups downstream of the PR-B transcription start site. This methylation change was not associated with altered PR-B expression, but may contribute to the increase in PR-A expression with labour. Chromatin immunoprecipitation revealed that the histone methyltransferase, SET and MYND domain-containing protein 3 (SMYD3), bound to the PR gene at significantly higher levels at the PR-A promoter compared with the PR-B promoter (P < 0.010), with no labour-associated changes observed. The H3K4 demethylase, Jumonji AT-rich interactive domain 1A (JARID1A), also bound to the PR-A, but not to the PR-B promoter prior to term labour, and decreased significantly at the onset of labour (P = 0.014), providing a mechanism for the previously reported increase in H3K4me3 level and PR-A expression with labour. Our studies suggest that epigenetic changes mediated by JARID1A, SMYD3 and DNA methylation may be responsible, at least in part, for the functional progesterone withdrawal that precipitates human labour. © The Author 2014. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved.

DOI 10.1093/molehr/gau005
Citations Scopus - 3Web of Science - 2
Co-authors John Fitter, Roger Smith
2013 Smith R, Zakar T, Madsen G, 'Mammalian Labor: Variations on a Theme by Amniota', ENDOCRINOLOGY, 154 584-588 (2013) [C1]
DOI 10.1210/en.2012-2262
Co-authors Roger Smith
2012 Smith R, Paul JW, Maiti K, Tolosa Gonzalez JM, Madsen GM, 'Recent advances in understanding the endocrinology of human birth', Trends in Endocrinology & Metabolism, 23 516-523 (2012) [C1]
Citations Scopus - 6Web of Science - 5
Co-authors Jonathan Paul, Roger Smith
2012 Chai S, Smith R, Zakar T, Mitchell CM, Madsen GM, 'Term myometrium is characterized by increased activating epigenetic modifications at the progesterone receptor-A promoter', Molecular Human Reproduction, 18 401-409 (2012) [C1]
Citations Scopus - 8Web of Science - 5
Co-authors Roger Smith
2009 Merlino A, Welsh T, Erdonmez T, Madsen GM, Zakar T, Smith R, et al., 'Nuclear progesterone receptor expression in the human fetal membranes and decidua at term before and after labor', Reproductive Sciences, 16 357-363 (2009) [C1]
DOI 10.1177/1933719108328616
Citations Scopus - 18Web of Science - 17
Co-authors Roger Smith
2007 Madsen GM, Macintyre DA, Mesiano S, Smith R, 'Progesterone receptor or cytoskeletal protein?', Reproductive Sciences, 14 217-222 (2007) [C1]
DOI 10.1177/1933719107302380
Citations Scopus - 9Web of Science - 8
Co-authors Roger Smith
2006 Gill AW, Madsen GM, Knox M, Bisits AM, Giles WB, Tudehope D, et al., 'Neonatal neurodevelopmental outcomes following tocolysis with glycerol trinitrate patches', American Journal of Obstetrics and Gynecology, 195 484-487 (2006) [C1]
DOI 10.1016/j.ajog.2006.01.103
Citations Scopus - 3Web of Science - 6
Co-authors Roger Smith
2004 Madsen GM, Zakar T, Ku CY, Sanborn B, Smith R, Mesiano S, 'Prostaglandins Differentially Modulate Progesterone Receptor-A and -B Expression in Human Myometrial Cells: Evidence for Prostaglandin-Induced Functional Progesterone Withdrawal', Endocrinology and Metabolism, 89 1010-1013 (2004) [C1]
DOI 10.1210/jc.2003-031037
Citations Scopus - 66Web of Science - 55
Co-authors Roger Smith
2004 Bisits AM, Madsen GM, Knox M, Gill AW, Smith R, Yeo GS, et al., 'The Randomized Nitric Oxide Tocolysis Trial (RNOTT) for the treatment of preterm labor', American Journal of Obstetrics and Gynecology, 191 683-690 (2004) [C1]
DOI 10.1016/j.ajog.2004.02.019
Citations Scopus - 32Web of Science - 22
Co-authors Roger Smith
2004 Madsen GM, Zakar T, Manuelpillai U, Wallace E, Kwek K, Yeo GS, et al., 'Intracrine Control of Estrogen Action in Human Gestational Tissues at Parturition', Society for Gynecologic Investigation. Journal, 11 213-219 (2004) [C1]
DOI 10.1016/j.jsgi.2003.12.003
Citations Scopus - 5Web of Science - 5
Co-authors Roger Smith
2001 Leung T, Chung T, Madsen GM, Lam P, Sahota D, Smith R, 'Rate of rise in maternal plasma corticotrohpin-releasing hormone and its relation to gestational length', BJOG: An international Journal of Obstetrics and Gynaecology, 108 527-532 (2001) [C1]
Citations Scopus - 20Web of Science - 15
Co-authors Roger Smith
2000 Clifton VL, Qing G, Murphy VE, Schwartz J, Madsen GM, Smith R, 'Localization and Characterization of Urocortin during Human Pregancy', Placenta, 21 782-788 (2000) [C1]
Citations Scopus - 44Web of Science - 39
Co-authors Roger Smith, Vicki Clifton, Vanessa Murphy
2000 Leung TN, Chung T, Madsen GM, Lam C, Lam P, Walters W, Smith R, 'Analysis of mid-trimester corticotrophin-releasing hormone and a-fetoprotein concentrations for predicting pre-eclampsia', Human Reproduction, 15 1813-1818 (2000) [C1]
Citations Scopus - 21Web of Science - 19
Co-authors Roger Smith
2000 Giles W, Bisits A, Knox M, Madsen GM, Smith R, 'The effect of fetal fibronectin testing on admissions to a tertiary maternal-fetal medicine unit and cost savings', American Journal of Obstetrics and Gynecology, 182 439-442 (2000) [C1]
Co-authors Roger Smith
1999 Smith R, Wicking J, Bowman M, Belleoud A, Dubreuil G, Davies J, Madsen GM, 'Corticotropin-releasing hormone in chimpazee and gorilla pregnancies', Journal of clinical endocrinology and Metabolism, (Aug);84/8 2820-2825 (1999) [C1]
Citations Scopus - 52Web of Science - 44
Co-authors Maria Bowman, Roger Smith
1999 Leung TN, Chung T, Madsen GM, McLean M, Chang AMZ, Smith R, 'Elevated mid-trimester maternal coritcotropin-releasing hormone levels in pregnancies destined to deliver preterm before 34 weeks', British Jounal of Obsetrics and Gynecology, (Oct);106 1041-1046 (1999) [C1]
Citations Scopus - 60Web of Science - 48
Co-authors Roger Smith
1998 Bisits AM, Madsen GM, McLean MT, Ocallaghan S, Smith R, Giles WB, 'CRH- a biomechnaical predictor of preterm delivery in a pilot randomised trial - of the treatment of preterm labour', American Journal of Obstetrics & Gynecology, 178 862-866 (1998) [C1]
Co-authors Roger Smith
1998 Bisits A, Madsen GM, McLean M, Ocallaghan S, Smith R, Giles W, 'Corticotropin-releasing hormone:a biochemical predictor of preterm delivery in a pilot randomised trial of the treatment of preterm labour', American Journal of Obstetrics and Gynaecology, 178(4) 862 (1998) [C1]
Citations Scopus - 30Web of Science - 27
Co-authors Roger Smith
1998 Batey RG, Madsen GM, 'The carbohydrate-deficient transferrin test in hospital practice', Drug and Alcohol Review, 17 105-109 (1998) [C1]
1998 Chan EC, Falconer J, Madsen GM, Rice KC, Webster EL, Chrousos GP, Smith R, 'Corticotropin-releasing hormone type 1 receptor antagonist delays parturition in sheep', Endocrinology, 139(7) 3357-3360 (1998) [C1]
Citations Scopus - 55Web of Science - 46
Co-authors Roger Smith
1988 POWIS DA, MADSEN GM, TOROK TL, 'AUGMENTATION OF THE INDIRECT SYMPATHOMIMETIC ACTION OF TYRAMINE BY CARDIOACTIVE STEROIDS IS A CONSEQUENCE OF ELEVATED INTRACELLULAR SODIUM', NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY, 337 273-278 (1988)
Citations Scopus - 2Web of Science - 3
1986 Powis DA, Madsen GM, 'Unsuitability of the 86Rb+ uptake method for estimation of (Na+ + K+)-ATPase activity in innervated tissues', BBA - Biomembranes, 861 251-258 (1986)

(Na+ + K+)-ATPase activity was estimated by 86Rb+ uptake in dog saphenous vein to determine the validity of the technique in tissues that have a sympathetic innervation. When saph... [more]

(Na+ + K+)-ATPase activity was estimated by 86Rb+ uptake in dog saphenous vein to determine the validity of the technique in tissues that have a sympathetic innervation. When saphenous vein rings were incubated at 37°C in Krebs' solution containing 86Rb+, the cardenolide acetylstrophanthidin caused a concentration-dependent inhibition of Rb+ uptake. The threshold for inhibition was approx. 10 nM acetylstrophanthidin and the maximum effect was obtained at 9 µM. In the upper part of this concentration range (> 1 µM) acetylstrophanthidin released noradrenaline from the sympathetic nerve terminals associated with the tissue. In this upper part of the acetylstrophanthidin concentration range the a-adrenoceptor antagonist phentolamine (8 µM) reduced, by up to 25%, the degree of 86Rb+ uptake inhibition caused by the cardenolide. In other experiments, saphenous vein strips were loaded with 86Rb+ and perifused with Krebs' solution containing acetylstrophanthidin. At concentrations which release noradrenaline, acetylstrophanthidin increased the efflux of 86Rb+. Phentolamine (8 µM) prevented the acetylstrophanthidin-evoked efflux of the isotope as did prior in vitro denervation of 86Rb+ loaded strips with 6-hydroxydopamine. Exogenous noradrenaline (1-100 µM) added to the perifusing fluid also caused an efflux of 86Rb+ that was attenuated by phentolamine. The data indicate for dog saphenous vein that with low concentrations of acetylstrophanthidin the extent of 86Rb+ accumulation might accurately reflect prevailing (Na+ + K+)-ATPase activity. At higher concentrations of acetylstrophanthidin, however, noradrenaline is released from the nerve endings and causes 86Rb+ efflux from the smooth muscle cells consequent upon a-adrenoceptor activation. Since this efflux reduces the extent of Rb+ accumulation, measurement of the latter does not adequately reflect uptake mediated by the activity of (Na+ + K+)-ATPase. This is significant because in most applications of the 86Rb+ uptake method it is the estimate of Rb+ accumulation made in the presence of a high concentration of cardenolide that forms the basis of all subsequent calculations with respect to (Na+ + K+)- ATPase activity. © 1986.

DOI 10.1016/0005-2736(86)90427-X
Citations Scopus - 2
1986 POWIS DA, MADSEN GM, 'UNSUITABILITY OF THE RB-86(+) UPTAKE METHOD FOR ESTIMATION OF (NA+ + K+)-ATPASE ACTIVITY IN INNERVATED TISSUES', BIOCHIMICA ET BIOPHYSICA ACTA, 861 251-258 (1986)
DOI 10.1016/0005-2736(86)90427-X
Citations Web of Science - 4
1986 POWIS DA, MADSEN GM, 'ALPHA-ADRENOCEPTOR-MEDIATED INCREASE IN TENSION AND EFFLUX OF RB-86 IN DOG SAPHENOUS-VEIN', BLOOD VESSELS, 23 95-96 (1986)
Citations Web of Science - 1
Show 21 more journal articles

Conference (7 outputs)

Year Citation Altmetrics Link
2012 Chai S, Fitter JT, Smith R, Madsen GM, 'DNA methylation of progesterone receptor isoforms in term human myometrium', Abstracts. The Annual Scientific Meeting of the Endocrine Society of Australia and the Society for Reproductive Biology 2012, Gold Coast, QLD (2012) [E3]
Co-authors John Fitter, Roger Smith
2012 Mitchell CM, Chai S, Madsen GM, Hirst JJ, Zakar T, 'Bivalent epigenetic modifications regulate PTGS2 expression during gestation in the amnion', Reproductive Sciences, San Diego, CA (2012) [E3]
Co-authors Jon Hirst
2011 Chai SY, Smith R, Zakar T, Mitchell CM, Madsen GM, 'Term myometrium is characterized by increased activating epigenetic marks on the progesterone receptor A promoter', Reproductive Sciences, Miami Beach, Florida (2011) [E3]
Co-authors Roger Smith
2010 Chai S, Smith R, Madsen GM, 'Epigenetic regulation of progesterone receptor isoform expression in term human myometrium', The Endocrine Society of Australia Annual Scientific Meeting Proceedings and Abstract Book, Sydney (2010) [E3]
Co-authors Roger Smith
2004 Madsen GM, Zakar T, Chan EC, Yeo GS, Kwek K, Mesiano S, 'Prostaglandin Receptor Expression in Human Myometrium Before and After the Onset of Term Labor', Prostaglandin Receptor Expression in Human Myometrium Before and After the Onset of Term Labor, Texas (2004) [E3]
2001 Giles WB, Knox M, Madsen GM, Bisits AM, Gill AW, Smith R, et al., 'The randomised nitric oxide tocolysis trial', American Journal of Obstetrics and Gynecology, Reno, Nevada (2001) [E3]
1998 Giles WB, Knox M, Masden G, Bisits AM, Smith R, 'The effect of fetal fibronectin usage on admissions to a tertiary maternal fetal medicine unit and cost saving', American Journal of Obstetrics and Gynecology, Miami, 1998 (1998) [E1]
Citations Scopus - 41Web of Science - 33
Co-authors Roger Smith
Show 4 more conferences
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Grants and Funding

Summary

Number of grants 11
Total funding $507,577

Click on a grant title below to expand the full details for that specific grant.


20151 grants / $25,222

Regulation of estrogen activation in human parturition$25,222

Funding body: John Hunter Hospital Charitable Trust Fund

Funding body John Hunter Hospital Charitable Trust Fund
Project Team Doctor Gemma Madsen, Dr Sharyn Rayner
Scheme Research Grant
Role Lead
Funding Start 2015
Funding Finish 2015
GNo G1500609
Type Of Funding Grant - Aust Non Government
Category 3AFG
UON Y

20131 grants / $23,810

Epigenetic Regulation of Progesterone Receptors and the Onset of Labour$23,810

Funding body: John Hunter Hospital Charitable Trust Fund

Funding body John Hunter Hospital Charitable Trust Fund
Project Team Doctor Gemma Madsen, Dr Sharyn Rayner
Scheme Research Grant
Role Lead
Funding Start 2013
Funding Finish 2013
GNo G1300703
Type Of Funding Other Public Sector - State
Category 2OPS
UON Y

20122 grants / $44,454

The regulation of progesterone withdrawal in human parturition$34,454

Funding body: John Hunter Hospital Charitable Trust Fund

Funding body John Hunter Hospital Charitable Trust Fund
Project Team Doctor Gemma Madsen, Dr Sharyn Rayner
Scheme Research Grant
Role Lead
Funding Start 2012
Funding Finish 2012
GNo G1200642
Type Of Funding Other Public Sector - State
Category 2OPS
UON Y

Precellys Tissue Homogeniser$10,000

Funding body: NHMRC (National Health & Medical Research Council)

Funding body NHMRC (National Health & Medical Research Council)
Project Team Professor Roger Smith, Professor Eugenie Lumbers, Conjoint Professor Tam Zakar, Professor Jon Hirst, Conjoint Professor Ian Wright, Doctor Gemma Madsen, Doctor Kaushik Maiti
Scheme Equipment Grant
Role Investigator
Funding Start 2012
Funding Finish 2012
GNo G1100979
Type Of Funding Other Public Sector - Commonwealth
Category 2OPC
UON Y

20111 grants / $69,157

Research microscope, confocal ready nikon eclipse 90i microscope$69,157

Funding body: NHMRC (National Health & Medical Research Council)

Funding body NHMRC (National Health & Medical Research Council)
Project Team Professor Roger Smith, Conjoint Professor Tam Zakar, Professor Jon Hirst, Doctor Kaushik Maiti, Doctor Gemma Madsen, Professor Rodney Scott, Conjoint Professor Peter Wark, Laureate Professor Paul Foster, Professor Phil Hansbro, Conjoint Professor Ian Wright
Scheme Equipment Grant
Role Investigator
Funding Start 2011
Funding Finish 2011
GNo G1100024
Type Of Funding Other Public Sector - Commonwealth
Category 2OPC
UON Y

20101 grants / $303,750

Regulation of progesterone action in human parturition$303,750

Funding body: NHMRC (National Health & Medical Research Council)

Funding body NHMRC (National Health & Medical Research Council)
Project Team Doctor Gemma Madsen
Scheme Project Grant
Role Lead
Funding Start 2010
Funding Finish 2010
GNo G0190155
Type Of Funding Aust Competitive - Commonwealth
Category 1CS
UON Y

20091 grants / $18,286

Epigenetic regulation of progesterone receptor isoform expression in human myometrium$18,286

Funding body: John Hunter Hospital Charitable Trust Fund

Funding body John Hunter Hospital Charitable Trust Fund
Project Team Doctor Gemma Madsen
Scheme Research Grant
Role Lead
Funding Start 2009
Funding Finish 2009
GNo G0190236
Type Of Funding Other Public Sector - State
Category 2OPS
UON Y

20081 grants / $15,208

Regulation of the differential expression of the A and B isoforms of the progesterone receptor in human parturition$15,208

Funding body: John Hunter Hospital Charitable Trust Fund

Funding body John Hunter Hospital Charitable Trust Fund
Project Team Doctor Gemma Madsen, Doctor Fatima Patel
Scheme Research Grant
Role Lead
Funding Start 2008
Funding Finish 2008
GNo G0189518
Type Of Funding Other Public Sector - State
Category 2OPS
UON Y

20051 grants / $1,220

Society of Gynecologic Investigation 1st International Summit on Preterm Birth, 10-12 November 2005$1,220

Funding body: University of Newcastle

Funding body University of Newcastle
Project Team Doctor Gemma Madsen
Scheme Travel Grant
Role Lead
Funding Start 2005
Funding Finish 2005
GNo G0185914
Type Of Funding Internal
Category INTE
UON Y

20041 grants / $1,470

Society of Gynecologic Investigation 51st Annual Scientific Meeting, 24 to 27 March 2004$1,470

Funding body: University of Newcastle

Funding body University of Newcastle
Project Team Doctor Gemma Madsen
Scheme Travel Grant
Role Lead
Funding Start 2004
Funding Finish 2004
GNo G0183892
Type Of Funding Internal
Category INTE
UON Y

20001 grants / $5,000

Role of estrogen receptors (ERa and ERb) in the regulation of human parturition.$5,000

Funding body: ARC (Australian Research Council)

Funding body ARC (Australian Research Council)
Project Team Doctor Sam Mesiano, Doctor Gemma Madsen
Scheme Small Grant
Role Investigator
Funding Start 2000
Funding Finish 2000
GNo G0178902
Type Of Funding Scheme excluded from IGS
Category EXCL
UON Y
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Research Supervision

Current Supervision

Commenced Research Title / Program / Supervisor Type
2013 Epigenetic Regulation of Progesterone Receptors and the Onset of Labour
Medical Studies, Faculty of Health and Medicine
Co-Supervisor
2009 Epigenetic Regulation of Progesterone Receptor Isoforms in Human Paturition
Medical Studies, Faculty of Health and Medicine
Principal Supervisor
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Dr Gemma Madsen

Position

Senior Research Officer
Mothers and Babies Research Centre
School of Medicine and Public Health
Faculty of Health and Medicine

Focus area

Reproductive Medicine

Contact Details

Email gemma.madsen@newcastle.edu.au
Phone (02) 4042 0359
Fax (02) 4042 0045

Office

Room 3403-HMRI (Mothers and Babies Research Centre)
Building HMRI - John Hunter Hospital
Location HMRI-John Hunter Hospital

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