Dr Kavita Pabreja

Dr Kavita Pabreja

Research Associate

School of Medicine and Public Health

Career Summary

Biography

Dr. Pabreja is an early career researcher and post-doctoral Research Associate with Priority Research Centre for Healthy Lungs under the leadership of Professor Jodie L Simpson.

Dr. Pabreja is involved in working on research projects investigating the role of the immune system, chronic inflammation and bacterial persistence in patients with asthma. On her research career journey, Dr. Pabreja has developed a wide range of appropriate skills and knowledge throughout the principles of biology, pharmacology and drug discovery covering extensive graduate and post-graduate training. 

Dr. Pabreja's doctoral research emphasized on utilizing the potential of new generation sequencing (NGS) technology, RNA-Seq, to understand the downstream signaling pathways associated with GLP-1R in diabetes and identifying novel targets that can be developed as effective therapeutics. Additionally, her postgraduate (MPharm) research focused on the understanding the pathogenesis underlying diabetic neuropathic pain and inflammation. 

With a background in chronic diseases and associated inflammation, she is well on the way in establishing herself in the field of chronic and inflammatory airway diseases. 


Qualifications

  • Doctor of Philosophy, Monash University
  • Master of Pharmacy, Punjab Technical University

Keywords

  • Asthma
  • Biochemical analysis
  • Cell death and proliferation
  • Efferocytosis
  • Inflammation
  • Inflammatory mediators
  • Macrophages
  • Neuropathy
  • Neutrophils
  • Non-typeable Haemophilus influenzae
  • Phagocytosis
  • Receptor Biology
  • Type II Diabetes

Languages

  • English (Fluent)
  • Hindi (Mother)

Fields of Research

Code Description Percentage
110203 Respiratory Diseases 100

Professional Experience

UON Appointment

Title Organisation / Department
Research Associate University of Newcastle
School of Medicine and Public Health
Australia
Research Associate Priority Research Centre (PRC) for Healthy Lungs | The University of Newcastle
School of Medicine and Public Health
Australia
Research Associate University of Newcastle
School of Medicine and Public Health
Australia

Professional appointment

Dates Title Organisation / Department
9/09/2016 -  Research Associate Priority Research Centre (PRC) for Healthy Lungs | The University of Newcastle
School of Medicine and Public Health
Australia
9/09/2016 -  Research Associate University of Newcastle
School of Medicine and Public Health
Australia
13/07/2015 - 7/09/2016 Research Assistant Priority Research Centre (PRC) for Healthy Lungs | The University of Newcastle
School of Medicine and Public Health
Australia
13/07/2015 - 7/09/2016 Research Assistant University of Newcastle
School of Medicine and Public Health
Australia

Teaching appointment

Dates Title Organisation / Department
2/02/2015 - 10/07/2015 Sessional Teaching Associate Monash University
Faculty of Pharmacy & Pharmaceutical Sciences, The Monash Institute of Pharmaceutical Sciences
Australia
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Publications

For publications that are currently unpublished or in-press, details are shown in italics.


Chapter (1 outputs)

Year Citation Altmetrics Link
2018 Awasthi R, Manchanda S, Das P, Velu V, Malipeddi H, Pabreja K, et al., 'Poly(vinylpyrrolidone)', Engineering of Biomaterials for Drug Delivery Systems: Beyond Polyethylene Glycol, Woodhead Publishing, Duxford, England 255-272 (2018) [B1]
DOI 10.1016/B978-0-08-101750-0.00009-X
Citations Scopus - 1

Journal article (18 outputs)

Year Citation Altmetrics Link
2018 Tiwari J, Gupta G, De Jesus Andreoli Pinto T, Sharma R, Pabreja K, Matta Y, et al., 'Role of microRNAs (miRNAs) in the pathophysiology of Diabetes mellitus', Panminerva Medica, 60 25-28 (2018) [C1]

© 2017 EDIZIONI MINERVA MEDICA. Diabetes mellitus is becoming the critical problem among the entire world and it is difficult to understand the molecular mechanism representing th... [more]

© 2017 EDIZIONI MINERVA MEDICA. Diabetes mellitus is becoming the critical problem among the entire world and it is difficult to understand the molecular mechanism representing the concept of diabetic pathology. Recently the knowledge of the involvement of genetics in type 2 diabetes mellitus (T2DM) susceptibility has sketched a great concentration towards the transcriptional activity of ß cells within the pancreas. This disease becomes the leading cause of death, so it is necessary to study the molecular pathogenesis, phenotypes, and characteristics to design the therapeutic parameters. Here in this review role of miRNA is being illustrated as it plays a crucial role in the pathogenesis, progression, and fate of beta cells of pancreas regulating the insulin secretion. Here in this review, we try to include the effects and pathophysiology of various miRNA in diabetes mellitus and on the various sites of the human body.

DOI 10.23736/S0031-0808.17.03382-1
Citations Scopus - 1
2017 Tiwari J, Gupta G, Dahiya R, Pabreja K, Sharma RK, Mishra A, Dua K, 'RECENT UPDATE ON BIOLOGICAL ACTIVITIES AND PHARMACOLOGICAL ACTIONS OF LIRAGLUTIDE', EXCLI JOURNAL, 16 742-747 (2017)
DOI 10.17179/excli2017-323
Citations Scopus - 1Web of Science - 1
2017 Gupta G, Chellappan DK, Agarwal M, Ashwathanarayana M, Nammi S, Pabreja K, Dua K, 'Pharmacological evaluation of the recuperative effect of morusin against aluminium trichloride (AlCl

© 2017 Bentham Science Publishers. Background: Elevation in brain levels of aluminium can be neurotoxic and can cause learning and memory deficiencies. In Chinese medicine, Morus ... [more]

© 2017 Bentham Science Publishers. Background: Elevation in brain levels of aluminium can be neurotoxic and can cause learning and memory deficiencies. In Chinese medicine, Morus alba is used as a neuroprotective herb. The current study was intended to discover the recuperative effect of morusin against aluminium trichloride (AlCl3)-induced memory impairment in rats along with biochemical mechanism of its protective action. Methods: Memory deficiency was produced by AlCl3 (100 mg/kg; p.o.) in experimental animals. Learning and memory activity was measured using Morris water maze (MWM) test model. Central cholinergic activity was evaluated through the measurement of brain acetylcholinesterase (AChE) activity. In addition to the above, oxidative stress was determined through assessment of brain thiobarbituric acid-reactive species (TBARS) and glutathione (GSH) levels. Results: AlCl3 administration prompted significant deficiency of learning and memory in rats, as specified by a noticeable reduction in MWM presentation. AlCl3 administration also produced a significant deterioration in brain AChE action and brain oxidative stress (increase in TBARS and decrease in GSH) levels. Treatment with morusin (5.0 and 10.0 mg/kg, dose orally) significantly overturned AlCl3-induced learning and memory shortages along with diminution of AlCl3-induced rise in brain AChE activity and brain oxidative stress levels. Conclusion: It may be concluded that morusin exerts a memory-preservative outcome in mental discrepancies of rats feasibly through its various activities.

DOI 10.2174/1871524917666161111095335
Citations Scopus - 3
2017 Pabreja K, Gibson P, Lochrin AJ, Wood L, Baines KJ, Simpson JL, 'Sputum colour can identify patients with neutrophilic inflammation in asthma', BMJ Open Respiratory Research, 4 (2017) [C1]

© Article author(s) (or their employer(s) unless otherwise stated in the text of the article) 2017. All rights reserved. Introduction Sputum colour is associated with neutrophilic... [more]

© Article author(s) (or their employer(s) unless otherwise stated in the text of the article) 2017. All rights reserved. Introduction Sputum colour is associated with neutrophilic inflammation in chronic bronchitis and chronic obstructive pulmonary disease (COPD). Neutrophilia and sputum expectoration is notable in asthma, but whether sputum colour is associated with and predicts the presence of neutrophilic inflammation in asthma is unknown. The objective of the study is to assess the ability of sputum colour in distinguishing asthma inflammatory phenotypes. Methods Induced sputum samples collected from 271 adults with stable asthma were retrospectively assessed. Sputum colour was determined using the BronkoTest sputum colour chart and correlated to differential cell counts and CXCL-8 concentration. Neutrophilic inflammation was defined as an age-corrected sputum neutrophil proportion (=61.6% for age 20¿40 years; =63.2% for age 40¿60 and =67.2% for age >60 years), whereas neutrophilic bronchitis (NB) was defined as high total cell count (=5.1×106cells/mL) plus an increased age-corrected neutrophil proportion. The optimal cut-off for sputum colour to predict neutrophilic inflammation and NB was determined using receiver operator characteristic curve analysis. Results A sputum colour score of =3 represented and predicted neutrophilic inflammation with modest accuracy (area under the curve (AUC)=0.64; p<0.001, specificity=78.4%, sensitivity=49.2%). Participants with a sputum colour score of =3 had significantly (p<0.05) higher CXCL-8, total cells and neutrophil number and proportion. Sputum colour score was also positively correlated with these factors. Sputum colour score =3 predicted NB with reasonably good accuracy (AUC=0.79, p<0.001, specificity=79.3%, sensitivity=70.7%). Conclusions Visual gradation of sputum colour in asthma relates to high total cell count and neutrophilic inflammation. Assessment of sputum colour can identify adults with asthma who are likely to have NB without the need for sputum processing and differential cell count, which may facilitate asthma management.

DOI 10.1136/bmjresp-2017-000236
Citations Scopus - 3
Co-authors Jodie Simpson, Peter Gibson, Lisa Wood, Katherine Baines
2017 Gupta G, Chellappan DK, Kikuchi IS, Pinto TDJA, Pabreja K, Agrawal M, et al., 'Nephrotoxicity in rats exposed to paracetamol: The protective role of moralbosteroid, a steroidal glycoside', Journal of Environmental Pathology, Toxicology and Oncology, 36 113-119 (2017) [C1]

© 2017 Begell House, Inc. Paracetamol (PCM) has an acceptable safety profile when used at prescribed doses. However, it is now understood that paracetamol can damage the kidneys w... [more]

© 2017 Begell House, Inc. Paracetamol (PCM) has an acceptable safety profile when used at prescribed doses. However, it is now understood that paracetamol can damage the kidneys when administered as an overdose. In addition, oxidative stress can play a major role in causing nephrotoxicity. This investigation studies the efficacy of moralbosteroid isolated from M. alba stem bark. Nephrotoxicity was induced with administration of paracetamol. Nephroprotection was studied using two doses of the extract. The experimental animals were divided into four groups (n = 6). Two groups served as positive and negative controls, respectively, and two received the test substances. All of the contents were orally administered. Significant reductions in nephrotoxicity and oxidative damages were observed in the treatment groups. There was a marked decrease in blood levels of urea, creatinine, and lipid peroxidation. Furthermore, it was found that glutathione levels in the blood increased dramatically after treatment. Histological findings confirmed the potent renoprotective potential of moralbosteroid. This was evidenced by the minimized intensity of nephritic cellular destruction. In animal studies, moralbosteroid exhibited dose-dependent activity, which is thought to be mediated through its antioxidant potential.

DOI 10.1615/JEnvironPatholToxicolOncol.2017019457
Citations Scopus - 3Web of Science - 2
2012 Dua K, Pabreja K, Gorajana A, 'Dissolution behaviour of aceclofenac-PVP coprecipitates', Ars Pharmaceutica, 53 7-12 (2012)

Aim: The objective of the present investigation was to study the effect of PVP on in vitro dissolution of aceclofenac from coprecipitates. Materials and Methods: Aceclofenac copre... [more]

Aim: The objective of the present investigation was to study the effect of PVP on in vitro dissolution of aceclofenac from coprecipitates. Materials and Methods: Aceclofenac coprecipitates (CP) with different drug loadings were prepared and in vitro dissolution studies of pure drug, physical mixtures and coprecipitates were carried out. Results: Coprecipitates of aceclofenac with PVP showed considerable increase in the dissolution rate in comparison with physical mixture and pure drug in 0.1 N HCl, pH1.2 and phosphate buffer, pH, 7.4. Coprecipitates in 1:2 ratio showed maximum dissolution rate in comparison to other ratios. Amorphous nature of the drug in coprecipitates was confirmed by scanning electron microscopy and a decrease in enthalpy of drug melting in coprecipitates compared to the pure drug. FT- IR spectroscopy and differential scanning calorimetry studies indicated no interaction between aceclofenac and PVP in coprecipitates in solid state. Dissolution enhancement was attributed to decreased crystallinity of the drug and to the wetting, eutectic formation and solubilizing effect of the carrier from the coprecipitates of aceclofenac. Conclusion: dissolution of aceclofenac can be enhanced by the use of hydrophilic carriers like PVP.

2012 Gorajana A, Rajendran A, Dua K, Pabreja K, Hoon TP, 'Preparation, Characterization, and In Vitro Evaluation of Nitrendipine Solid Dispersions', Journal of Dispersion Science and Technology, 33 676-684 (2012)

In order to enhance the absorption of dissolution rate of nitrendipine (NIT), solid dispersions were prepared using two water soluble carriers, polyvinylpyrrolidone K30 (PVP K30) ... [more]

In order to enhance the absorption of dissolution rate of nitrendipine (NIT), solid dispersions were prepared using two water soluble carriers, polyvinylpyrrolidone K30 (PVP K30) and polyethylene glycol 4000 (PEG-4000), by solvent and fusion method. The dissolution profile of solid dispersions were compared to the pure drug in both pH 1.2 hydrochloric acid and pH 6.8 phosphate buffer and the results have shown profound improvement in drug release. The solubility of solid dispersion was increased by several folds when compared to pure NIT. The physicochemical properties of the solid dispersions were examined using analytical techniques. The results of microscopic studies, x-ray powder diffraction (XRPD), and differential scanning calorimetric (DSC) analysis confirmed the amorphous state of solid dispersion in comparison to the crystalline nature of pure drug, proposing that NIT was molecularly dispersed in the polymer matrices, which were accounted for by dissolution rate enhancement. Fourier transform infrared (FTIR) spectroscopic analysis indicated the presence of hydrogen bonding between NIT and the polymers, which also explained the improvement in solubility and dissolution rate. In conclusion, solid dispersion of NIT with PVP K30 and PEG-4000 improved the solubility and rate of dissolution, which may improve the absorption of the drug and subsequently the bioavailability of NIT. © 2012 Copyright Taylor and Francis Group, LLC.

DOI 10.1080/01932691.2011.579829
Citations Scopus - 4
2012 Ramana MV, Dua K, Himaja M, Pabreja K, 'Preparation and characterization of solid dispersions of Rofecoxib', Anti-Inflammatory and Anti-Allergy Agents in Medicinal Chemistry, 10 393-398 (2012)

The present study is aimed at improving the dissolution of poorly soluble drug, rofecoxib, using solid dispersion technique. The solid dispersions were prepared in different propo... [more]

The present study is aimed at improving the dissolution of poorly soluble drug, rofecoxib, using solid dispersion technique. The solid dispersions were prepared in different proportions using hydrophilic carriers like mannitol, and urea. The dissolution rate studies were performed in both simulated gastric fluid and simulated intestinal fluid. It is observed that the dissolution was affected by the acidity of the medium. Solid dispersions gave faster dissolution rate when compared to corresponding physical mixture and pure drug. In vivo absorption and anti-inflammatory activity studies of solid dispersions also confirmed the above results. The DSC thermogram and IR spectra revealed that there is no interaction of Rofecoxib with additives and the drug, rofecoxib is stable in solid dispersions. © 2011 Bentham Science Publishers.

DOI 10.2174/1871523011109060393
Citations Scopus - 1
2011 Pabreja K, Dua K, Sharma S, Padi SSV, Kulkarni SK, 'Minocycline attenuates the development of diabetic neuropathic pain: Possible anti-inflammatory and anti-oxidant mechanisms', European Journal of Pharmacology, 661 15-21 (2011)

Painful neuropathy, a common complication of diabetes mellitus is characterized by allodynia and hyperalgesia. Recent studies emphasized on the role of non-neuronal cells, particu... [more]

Painful neuropathy, a common complication of diabetes mellitus is characterized by allodynia and hyperalgesia. Recent studies emphasized on the role of non-neuronal cells, particularly microglia in the development of neuronal hypersensitivity. The purpose of the present study is to evaluate the effect of minocyline, a selective inhibitor of microglial activation to define the role of neuroimmune activation in experimental diabetic neuropathy. Cold allodynia and thermal and chemical hyperalgesia were assessed and the markers of inflammation and oxidative and nitrosative stress were estimated in streptozotocin-induced diabetic rats. Chronic administration of minocycline (40 and 80 mg/kg, i.p.) for 2 weeks started 2 weeks after diabetes induction attenuated the development of diabetic neuropathy as compared to diabetic control animals. In addition, minocyline treatment reduced the levels of interleukin-1ß and tumor necrosis factor-a, lipid peroxidation, nitrite and also improved antioxidant defense in spinal cords of diabetic rats as compared to diabetic control animals. In contrast, minocycline (80 mg/kg, per se) had no effect on any of these behavioral and biochemical parameters assessed in age-matched control animals. The results of the present study strongly suggest that activated microglia are involved in the development of experimental diabetic neuropathy and minocycline exerted its effect probably by inhibition of neuroimmune activation of microglia. In addition, the beneficial effects of minocycline are partly mediated by its anti-inflammatory effect by reducing the levels of proinflammatory cytokines and in part by modulating oxidative and nitrosative stress in the spinal cord that might be involved in attenuating the development of behavioral hypersensitivity in diabetic rats. © 2011 Elsevier B.V. All rights reserved.

DOI 10.1016/j.ejphar.2011.04.014
Citations Scopus - 79
2011 Dua K, Pabreja K, Ramana MV, Bukhari NI, 'Preparation, characterization, and in vitro evaluation of aceclofenac PVP-solid dispersions', Journal of Dispersion Science and Technology, 32 1151-1157 (2011)

The objective of the present investigation was to study the effect of polyvinylpyrrolidone (PVP) on in vitro dissolution of aceclofenac from solid dispersions. Aceclofenac binary ... [more]

The objective of the present investigation was to study the effect of polyvinylpyrrolidone (PVP) on in vitro dissolution of aceclofenac from solid dispersions. Aceclofenac binary solid dispersions (SD) with different drug loadings were prepared using the melting or fusion method. In vitro dissolution of pure drug, physical mixtures, and solid dispersions were carried out. Solid dispersion of aceclofenac with PVP showed considerable increase in the dissolution rate in comparison with physical mixture and pure drug in 0.1N HCl, pH 1.2 and phosphate buffer, pH 7.4. Solid dispersions in 1:2 ratio showed maximum dissolution rate in comparison to other ratios. The amorphous nature of the drug in solid dispersion was confirmed by scanning electron microscopy and a decrease in enthalpy of drug melting in solid dispersion compared to the pure drug. FTIR spectroscopy and differential scanning calorimetry studies indicated no interaction between aceclofenac and PVP in solid dispersions in solid state. Dissolution enhancement was attributed to decreased crystallinity of the drug and to the wetting, eutectic formation, and solubilizing effect of the carrier from the solid dispersions of aceclofenac. In conclusion, dissolution of aceclofenac can be enhanced by the use of hydrophilic carriers like PVP. © Taylor & Francis Group, LLC.

DOI 10.1080/01932691.2010.498239
Citations Scopus - 3
2011 Dua K, Pabreja K, Ramana MV, 'Enhancement of dissolution behavior of aceclofenac by complexation with ß-cyclodextrin-choline dichloride coprecipitate', Journal of Dispersion Science and Technology, 32 1477-1484 (2011)

The objective of the present investigation was to study the effect of presence of choline dichloride (CDC) in ß-cyclodextrin (ß-CD) on in vitro dissolution of aceclofenac (AF) fro... [more]

The objective of the present investigation was to study the effect of presence of choline dichloride (CDC) in ß-cyclodextrin (ß-CD) on in vitro dissolution of aceclofenac (AF) from molecular inclusion complexes. The molecular inclusion complexes of AF with ß-CD coprecipitated with CDC in 1:1 and 1:2 M ratio were prepared using kneading method. In vitro dissolution of pure drug, physical mixtures, and cyclodextrin inclusion complexes (AF-ß-CD-CDC) were carried out. Molecular inclusion complexes of aceclofenac with coprecipitated ß-CD showed considerable increase in the dissolution rate in comparison with physical mixture and pure drug in 0.1 N HCl, pH 1.2 and phosphate buffer, pH, 7.4. Inclusion complexes with 1:2 M ratio showed maximum dissolution rate in comparison to other ratios. FTIR spectroscopy and differential scanning calorimetry studies indicated no interaction between AF and ß-CD-CDC in complexes in solid state. Dissolution enhancement was attributed to the formation of water soluble inclusion complexes with the precipitated form of b-CD. The in vitro release from all the formulations was best described by first order kinetics (R2=1/4 0.9354 and 0.9268 in 0.1 N HCl and phosphate buffer, respectively) followed by Higuchi release model (R2=1/4 0.9029 and 0.9578 in 0.1 N HCl and phosphate buffer, respectively). In conclusion, dissolution of aceclofenac can be enhanced by using the ß-CD-CDC coprecipitate as a host molecule. © Taylor & Francis Group, LLC.

DOI 10.1080/01932691.2010.513317
Citations Scopus - 6
2011 Pabreja K, Dua K, Gorajana A, 'Evaluation of topical gels containing ketorolac tromethamine on inflammation and hyperalgesia in rats', Anti-Inflammatory and Anti-Allergy Agents in Medicinal Chemistry, 10 323-326 (2011)

Over recent years, non-steroidal anti-inflammatory drugs (NSAIDs) have been increasingly introduced as topical preparations as they are simple to apply and deliver high drug conce... [more]

Over recent years, non-steroidal anti-inflammatory drugs (NSAIDs) have been increasingly introduced as topical preparations as they are simple to apply and deliver high drug concentrations locally with limited side effects. Ketorolac trometamol (KT), a potent COX-2 inhibitor, produces typical side effects of NSAIDs when given orally and systemically. Hence the present investigation encompasses the development of topical formulations employing different dermatological bases and evaluated for its efficacy and safety. Standard procedures were followed to test the anti-inflammatory and antihyperalgesic effects in male Wistar albino rats. Amongst the various semisolid formulations, the formulation containing hydroalcoholic carbopol gel base (KT 1) was found to be significantly (p < 0.05) more effective in inhibiting hyperalgesia associated with inflammation (79.69±1.51 after 5 h of carrageenan administration) as compared to formulation containing plain carbopol gel base (68.75±2.76) and PEG base 73.44±1.23. This demonstrates the suitability of carbopol gel base as an ideal dermatological base for ketorolac trometamol topical formulation and thus providing an ample credence for better therapeutic efficacy. © 2011 Bentham Science Publishers.

2011 Dua K, Pabreja K, Ramana MV, Lather V, 'Dissolution behavior of ß-cyclodextrin molecular inclusion complexes of aceclofenac', Journal of Pharmacy and Bioallied Sciences, 3 417-425 (2011)

The objective of the present investigation was to study the effect of-cyclodextrin (-CD) on the in vitro dissolution of aceclofenac (AF) from molecular inclusion complexes. Aceclo... [more]

The objective of the present investigation was to study the effect of-cyclodextrin (-CD) on the in vitro dissolution of aceclofenac (AF) from molecular inclusion complexes. Aceclofenac molecular inclusion complexes in 1:1 and 1:2 M ratio were prepared using a kneading method. The in vitro dissolution of pure drug, physical mixtures, and cyclodextrin inclusion complexes was carried out. Molecular inclusion complexes of AF with-CD showed a considerable increase in the dissolution rate in comparison with the physical mixture and pure drug in 0.1 N HCl, pH 1.2, and phosphate buffer, pH 7.4. Inclusion complexes with a 1:2 M ratio showed the maximum dissolution rate in comparison to other ratios. Fourier transform infrared spectroscopy and differential scanning calorimetry studies indicated no interaction between AF and-CD in complexes in solid state. Molecular modeling results indicated the relative energetic stability of the-CD dimer-AF complex as compared to-CD monomer-AF. Dissolution enhancement was attributed to the formation of water soluble inclusion complexes with-CD. The in vitro release from all the formulations was best described by first-order kinetics (R2= 0.9826 and 0.9938 in 0.1 N HCl and phosphate buffer, respectively) followed by the Higuchi release model (R2= 0.9542 and 0.9686 in 0.1 N HCl and phosphate buffer, respectively). In conclusion, the dissolution of AF can be enhanced by the use of a hydrophilic carrier like-CD.

DOI 10.4103/0975-7406.84457
Citations Scopus - 27
2010 Dua K, Pabreja K, Ramana MV, 'Comparative Investigation on in vitro release of extemporaneously prepared norfloxacin semisolid formulations with marketed silver sulfadiazine 1% cream, USP using model independent approach', Ars Pharmaceutica, 51 177-185 (2010)

Objective: In an attempt for better treatment of bacterial infections, various semisolid formulations containing 5% w/w of norfloxacin were prepared and evaluated for in vitro dru... [more]

Objective: In an attempt for better treatment of bacterial infections, various semisolid formulations containing 5% w/w of norfloxacin were prepared and evaluated for in vitro drug release and in vitro skin permeability using dialysis membrane and rat abdominal skin respectively. The in vitro diffusion and permeation profile of the prepared formulation was compared with marketed silver sulfadiazine cream 1%, USP using model independent approach. Methods: Various semisolid formulations were prepared with different dermatological bases using standard procedures. In vitro diffusion and permeation studies were carried out using Keshary-Chein (KC) type diffusion cell using dialysis membrane and rat abdominal skin respectively. Results: The f1 lower than 15 and f2 higher than 50 indicated similarities in the in vitro diffusion and permeation profiles of the extemporaneously prepared selected semisolid formulations and marketed silver sulfadiazine 1% cream, USP. Conclusion: Amongst all the semisolid formulations prepared, carbopol gel base was found to be most suitable dermatological base for norfloxacin, the results obtained for in vitro diffusion, and in vitro skin permeation studies are comparable with that of marketed silver sulphadiazine 1% cream, USP.

Citations Scopus - 5
2010 Dua K, Pabreja K, Ramana M, 'Aceclofenac topical dosage forms: In vitro and in vivo characterization', Acta Pharmaceutica, 60 467-478 (2010)

Aceclofenac is a new generation non-steroidal anti-inflammatory drug showing effective anti-inflammatory and analgesic properties. It is available in the form of tablets of 100 mg... [more]

Aceclofenac is a new generation non-steroidal anti-inflammatory drug showing effective anti-inflammatory and analgesic properties. It is available in the form of tablets of 100 mg. Importance of aceclofenac as a NSAID has inspired development of topical dosage forms. This mode of administration may help avoid typical side effects associated with oral administration of NSAIDs, which have led to its withdrawal. Furthermore, aceclofenac topical dosage forms can be used as a supplement to oral therapy for better treatment of conditions such as arthritis. Ointments, creams, and gels containing 1% (m/m) aceclofenac have been prepared. They were tested for physical appearance, pH, spreadability, extrudability, drug content uniformity, in vitro diffusion and in vitro permeation. Gels prepared using Carbopol 940 (AF<sub>2</sub>, AF<sub>3</sub>) and macrogol bases (AF<sub>7</sub>) were selected after the analysis of the results. They were evaluated for acute skin irritancy, anti-inflammatory and analgesic effects using the carrageenan-induced thermal hyperalgesia and paw edema method. AF<sub>2</sub> was shown to be significantly (p < 0.05) more effective in inhibiting hyperalgesia associated with inflammation, compared to AF<sub>3</sub> and AF<sub>7</sub>. Hence, AF<sub>2</sub> may be suggested as an alternative to oral preparations.

DOI 10.2478/v1007-010-0036-5
Citations Scopus - 24
2010 Pabreja K, Dua K, Padi SSV, 'Evaluation of extemporaneously manufactured topical gels containing aceclofenac on inflammation and hyperalgesia in rats', Current Drug Delivery, 7 324-328 (2010)

The systemic use of non-steroidal anti-inflammatory drugs (NSAIDs) which act by inhibiting cyclooxygenase (COX) is severely hampered by gastric and peptic ulcers. The topical deli... [more]

The systemic use of non-steroidal anti-inflammatory drugs (NSAIDs) which act by inhibiting cyclooxygenase (COX) is severely hampered by gastric and peptic ulcers. The topical delivery of NSAIDs has the advantages of avoiding gastric and peptic ulcers and delivering the drug to the inflammation site. Importance of aceclofenac as a new generational NSAID has inspired the development of topical dosage forms. This mode of administration may help to avoid typical side effects of NSAIDs associated with oral and systemic administration such as gastric irritation, particularly diarrhoea, nausea, abdominal pain and flatulence. The aim of this study was to formulate topical gel containing 1% of aceclofenac in carbopol and PEG base and to evaluate it for analgesic and antiinflammatory activity using carrageenan-induced thermal hyperalgesia and paw oedema in rats. Carrageenan administration into the hind paw produced a significant inflammation associated with hyperalgesia as shown by decreased rat paw withdrawal latency in response to a thermal stimulus (47±0.5°C) 4 h after carrageenan injection. Topical application of AF1 significantly attenuated the development of hypersensitivity to thermal stimulus as compared to control (P<0.05) and other formulation treated groups (P<0.05). All the AF semisolid formulations, when applied topically 2 h before carrageenan administration, inhibited paw edema in a timedependent manner with maximum percent edema inhibition of 80.33±2.52 achieved with AF1 after 5 h of carrageenan administration However, topical application of AF2 markedly prevented the development of edema as compared to other formulation (AF2 and AF3) treated groups (P<0.05). Among all the semisolid formulations, Carbopol gel base was found to be most suitable dermatological base for aceclofenac. © 2010 Bentham Science Publishers Ltd.

DOI 10.2174/156720110793360649
Citations Scopus - 6
2009 Dua K, Sharma VK, Ramana MV, Sara UVS, Pabreja K, 'Developments in transdermal drug delivery', Australian Journal of Pharmacy, 90 69-71 (2009)
2007 Dua K, Ramana MV, Singh Sara UV, Himaja M, Agrawal A, Garg V, Pabreja K, 'Investigation of enhancement of solubility of norfloxacin ß-cyclodextrin in presence of acidic solubilizing additives', Current Drug Delivery, 4 21-25 (2007)

The present study is aimed at improving the solubility of a poorly water-soluble drug, norfloxacin by incorporating solubilizing additives such as ascorbic acid and citric acid in... [more]

The present study is aimed at improving the solubility of a poorly water-soluble drug, norfloxacin by incorporating solubilizing additives such as ascorbic acid and citric acid into the ß-cyclodextrin complexes. Norfloxacin, being amphoteric in nature, exhibits a higher solubility at pH below 4 and above 8. Addition of substances like ascorbic acid and citric acid in ß-cyclodextrin complexes reduces the pH of the immediate microenvironment of the drug below pH 4. In the present work, ß-cyclodextrin complexes of norfloxacin were prepared along with solubilizing additives such as citric acid and ascorbic acid in various proportion and the dissolution profile was performed in both HCl buffer, pH 1.2 and phosphate buffer, pH 7.4. The results have shown an enhanced dissolution rate in both media. DSC and IR spectral studies performed on the solid complexes have shown that there is no interaction of the drug with the additives and â-cyclodextrin. Disc diffusion studies have shown larger diameters of zone of inhibition indicating a greater diffusivity of the drug into the agar medium. © 2007 Bentham Science Publishers Ltd.

DOI 10.2174/156720107779314776
Citations Scopus - 36
Show 15 more journal articles

Conference (4 outputs)

Year Citation Altmetrics Link
2017 Erriah M, Pabreja K, Baines KJ, Fricker M, Simpson JL, 'Galectin-3 Expression In Monocyte-Derived Macrophages From Severe Asthmatics', AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, Washington, DC (2017)
Co-authors Michael Fricker, Jodie Simpson, Katherine Baines
2017 Simpson JL, Pabreja K, Baines KJ, Eyres F, Yang M, Nair P, et al., 'Sputum Il-27 Gene Expression In Asthma Endotypes', AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, Washington, DC (2017)
Co-authors Katherine Baines, Ming Yang, Peter Gibson, Jodie Simpson
2017 Pabreja K, Gibson PG, Baines KJ, Eyers F, Yang M, Nair P, et al., 'INCREASED EXPRESSION OF IL-27 IN NEUTROPHILIC ASTHMA', RESPIROLOGY (2017)
Co-authors Jodie Simpson, Paul Foster, Peter Gibson, Ming Yang, Katherine Baines
2017 Erriah M, Pabreja K, Baines KJ, Fricker M, Donnelly LE, Simpson JL, 'EFFEROCYTOSIS OF APOPTOTIC GRANULOCYTES BY MONOCYTE-DERIVED MACROPHAGES IN ADULTS WITH ASTHMA', RESPIROLOGY (2017)
Co-authors Katherine Baines, Michael Fricker, Jodie Simpson
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Grants and Funding

Summary

Number of grants 4
Total funding $28,728

Click on a grant title below to expand the full details for that specific grant.


20173 grants / $6,983

Enrolment in advanced or accelerated leadership program offered through The National Excellence in Educational Leadership Initiative (NEELI), Women & Leadership Australia (WLA)$3,000

Funding body: Hunter Medical Research Institute

Funding body Hunter Medical Research Institute
Project Team Doctor Kavita Pabreja
Scheme Equal Futures Award
Role Lead
Funding Start 2017
Funding Finish 2017
GNo G1701541
Type Of Funding Scheme excluded from IGS
Category EXCL
UON Y

European Respiratory Society (ERS) International Congress 2017 and Lab visit at The National Heart and Lung Institute, Imperial College London, UK$2,000

Funding body: Ian Potter Foundation

Funding body Ian Potter Foundation
Project Team Doctor Kavita Pabreja
Scheme Travel Grant
Role Lead
Funding Start 2017
Funding Finish 2017
GNo G1700106
Type Of Funding Scheme excluded from IGS
Category EXCL
UON Y

SDVCRI Cash Contribution$1,983

Funding body: University of Newcastle

Funding body University of Newcastle
Project Team Doctor Kavita Pabreja
Scheme Special Project Grant
Role Lead
Funding Start 2017
Funding Finish 2017
GNo G1701243
Type Of Funding Internal
Category INTE
UON Y

20161 grants / $21,745

Understanding the impact of antibiotic therapy on bacterial communities in severe asthma$21,745

Funding body: John Hunter Hospital Charitable Trust

Funding body John Hunter Hospital Charitable Trust
Project Team Doctor Kavita Pabreja, Professor Jodie Simpson, Conjoint Associate Professor Nick Saltos
Scheme Research Grant
Role Lead
Funding Start 2016
Funding Finish 2016
GNo G1600241
Type Of Funding Other Public Sector - State
Category 2OPS
UON Y
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Research Supervision

Number of supervisions

Completed1
Current0

Past Supervision

Year Level of Study Research Title Program Supervisor Type
2018 PhD Galectin-3 and Phagocyte Function in Asthma PhD (Medicine), Faculty of Health and Medicine, The University of Newcastle Co-Supervisor
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Dr Kavita Pabreja

Position

Research Associate
PRC for Healthy Lungs and VIVA
School of Medicine and Public Health
Faculty of Health and Medicine

Contact Details

Email kavita.pabreja@newcastle.edu.au
Phone 40420833
Fax 4042 0046

Office

Room HMRI Buiding, Level 2, West
Building HMRI Building
Location New Lambton Heights

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