Dr He Wang

Dr He Wang

Senior Lecturer

School of Health Sciences

Career Summary

Biography

I am a Senior Lecturer in Occupational in Health and Safety at the School of Health Sciences. I am also a Researcher interested in health effects of environmental and occupational factors.

RESEARCH and INNOVATION

My research focusses on environmental hazards and their health effects by investigating the toxicity of the environmental factors on organisms at molecular and cellular levels in cell, animal and human models.  My research includes the following areas:

  • Inflammatory and fibrotic effects of dust particles;
  • Carcinogenic effects of airborne particles;
  • Development of age-related diseases induced by environmental factors;
  • Smoking and cancer risk;
  • Environmental and occupational respiratory disease;
  • Mutagenic effects of nanoparticles;
  • Harmful effects of arsenic compounds;
  • Respiratory health effects of chemical mixtures;
  • Chemical induced autophagy, apoptosis and senescence and
  • Chemical induced gene expressions reflected by RNA-seq and proteomics.

The long term goal of my research is to effectively prevent environmental and occupational disorders from occurring. The objectives of my current research, to pursue my long term goal, are to detect early and reversible changes induced by environmental and occupational factors and address them in a timely manner.

I used cell, rat and human models in my studies. The hazards under study are mainly airborne particles including nano-sized hazards. My research results contributed significantly to relevant areas. For example, my rat model research contributed to the guidelines for National and World Leadership to Prevent Workplace Illnesses and Injuries led by WHO, NIOSH, United Nations Environment Program (UNEP), and International Labor Organization (ILO). Five articles authored by me have been cited by those organizations to make recommendations for the prevention of silica-related illness. For another example, my paper “Cyto- and genotoxicity of ultrafine TiO2 particles in cultured human lymphoblastoid cells”, has received outstanding citations, indicating the impact of this paper on the scientific community. The results of my research generated a list of publications and more than 1,100 citations so far.

TEACHING and LEARNING

I teach in the postgraduate Work Health and Safety programs at UON as a course coordinator and senior lecturer and played the same roles in the undergraduate BEnvOSH program taught in Singapore. In my teaching, I encourage deep approach by providing hypothetical cases and case analyses. I enjoy teaching and believe it will help to systemizing and expanding my own knowledge.

SERVICE and ENGAGEMENT

At UON I was program coordinator for our BEnvOSH program taught in Singapore during January 2015 to September 2015. I am editorial board member for the following international journals:

Journal of Toxicology and Environmental Health

Austin Journal of Environmental Toxicology

Austin Pharmacology & Pharmaceutics

Journal of Respiratory Medicine and Lung Disease

I am also a full member of American Society of Toxicology

INTERNATIONAL COLLABORATIONS

I am currently collaborating with American researchers in Tulane University and Xavier University for molecular toxicology studies. The research focus is gene expression changes induced by oil spill chemicals. A number of publications have been generated in such collaborations.


Qualifications

  • Doctor of Philosophy, University of Sydney

Keywords

  • Autophagy and Apoptosis
  • Black Lung
  • Cellular Senescence
  • Environmental and Occupational Respiratory Disease
  • Gene Environmental Interactions
  • Toxicity of Arsenic
  • Toxicity of Chemical Mixtures
  • Toxicity of Diesel Exhaust
  • Toxicity of Silica and asbestos
  • Toxicology of nanoparticles

Languages

  • Mandarin (Mother)
  • English (Fluent)

Fields of Research

Code Description Percentage
111705 Environmental and Occupational Health and Safety 70
040101 Atmospheric Aerosols 15
039901 Environmental Chemistry (incl. Atmospheric Chemistry) 15

Professional Experience

UON Appointment

Title Organisation / Department
Senior Lecturer University of Newcastle
School of Health Sciences
Australia

Academic appointment

Dates Title Organisation / Department
26/10/2007 - 10/12/2014 Assistant Professor of Environmental and Occupational Health Sciences

As a faculty member in the School of Public Health and Tropical Medicine, Dr. He Wang taught Occupational Health, Environmental Health Risk Assessment, Principles of Toxicology, Industrial Hygiene and Environmental Cancer Risk Inquiry for undergraduate, graduate and doctoral students. He also mentored master students, PhD candidates and postdoctoral fellows in toxicology research.

Tulane University
Global Environmental Health Sciences
United States
7/08/2003 - 18/10/2007 Senior Lecturer of Environmental and Occupational Health

As a senior lecturer, Dr Wang taught public health and public health sciences to undergraduate students. He also taught Diseases of Occupation, Practical Occupational health, Occupational Toxicology for postgraduate students. He also played role as program coordinator for Master of Public Health and Master of Occupational Health and Safety programs. Dr Wang supervised MPH coursework students and Master of Occupational Health and Safety coursework students in research. Dr He Wang also supervised a PhD candidate in toxicological research as a main supervisor.

Adelaide University
Public health
Australia

Professional appointment

Dates Title Organisation / Department
10/04/1998 - 3/08/2007 Scientific Officer

As a scientific officer, Dr Wang did research on respiratory health of coal miners, school children, newborn and premature infants, cystic fibrosis patients and patients with other respiratory diseases. Dr Wang was initially adjunct lecturer in the School and and promoted to adjunct senior lecturer in 2003.

The University of New South Wales
School of Women's and Children's Health
Australia

Teaching

Code Course Role Duration
OHSE6010 Occupational Health
The University of Newcastle - Faculty of Health and Medicine
The course focus is association between occupational hazards and occupational disorders
Lecturer and coordinator 23/02/2015 - 5/06/2015
OHSE6090 Research inOccupational Health and Safety
The University of Newcastle - Faculty of Health and Medicine
This course introduces aspects of occupational health and safety. Epidemiology is the focus of the course. The course also covers some other research methods and critical appraisal of the literature.
Lecturer and coordinator 27/07/2015 - 6/11/2015
OHSE3640 Research Methods in EOHS
The University of Newcastle, Singapore
This is a research methods course. It covers research methods to investigate the association between environmental/occupational factors and the harmful effects.
Lecturer 4/05/2015 - 31/07/2015
OHSE6010 Occupational Health
The University of Newcastle - Faculty of Health and Medicine
The course focus on the association between occupational hazards and occupational disorders.
Lecturer and coordinator 22/02/2016 - 3/06/2016
OHSE6090 Research inOccupational Health and Safety
The University of Newcastle - Faculty of Health and Medicine
The course focus is epidemiological methods for studying the association between occupational hazards and occupational disorders.
Lecturer and coordinator 25/07/2016 - 7/11/2016
OHSE3640 Research Methods in EOHS
The University of Newcastle, Singapore
This is a research course focusing on the relationship between environmental/occupational factors and harmful health effects.
Lecturer 2/05/2016 - 29/07/2016
OHSE2610 Occupational Health
The University of Newcastle, Singapore
This is a course focusing on the association between occupational hazards and occupational disorders.
Lecturer 2/05/2016 - 29/07/2016
OHSE6010 Occupational Health
The University of Newcastle - Faculty of Health and Medicine
This course introduces students to the relationship between health and the work environment. It illustrates aspects of the structure and function of the human body and investigates the recognition of how hazards in the workplace can affect the body.
Lecturer and Coordinator 27/02/2017 - 23/06/2017
OHSE6090 Research inOccupational Health and Safety
The University of Newcastle - Faculty of Health and Medicine
This course focuses on epidemiological methods to study the association between occupational hazards and occupational disorders.
Lecturer and Coordinator 24/07/2017 - 3/11/2017
OHSE3640 Research Methods in EOHS
The University of Newcastle, Singapore
This is a course focusing on the association between occupational hazards and occupational disorders.
Lecturer and Coordinator 1/05/2017 - 28/07/2017
OHSE2610 Occupational Health
The University of Newcastle, Singapore
This is a course focusing on the association between occupational hazards and occupational disorders.
Lecturer 1/05/2017 - 28/07/2017
OHSE6010 Occupational Health
The University of Newcastle - Faculty of Health and Medicine
This course introduces students to the relationship between health and the work environment. It illustrates aspects of the structure and function of the human body and investigates the recognition of how hazards in the workplace can affect the body.
Lecturer and Coordinator 26/02/2018 - 8/06/2018
Edit

Publications

For publications that are currently unpublished or in-press, details are shown in italics.


Journal article (63 outputs)

Year Citation Altmetrics Link
2018 Zhang M, Zheng H, Zhang X, Tian X, Xu S, Liu Y, et al., 'Involvement of nerve growth factor in mouse hippocampal neuronal cell line (HT22) differentiation and underlying role of DNA methyltransferases', Journal of toxicology and environmental health. Part A, 81 1116-1122 (2018)

DNA methylation is an epigenetic event involved in regulation of gene transcription during cell differentiation. DNA methyltransferases (DNMT) play a role in differentiation of ne... [more]

DNA methylation is an epigenetic event involved in regulation of gene transcription during cell differentiation. DNA methyltransferases (DNMT) play a role in differentiation of neural stem cells into neurons. The aim of this study was to determine whether nerve growth factor (NGF) was involved in differentiation of mouse hippocampal neuronal cell line (HT22) as assessed by IncuCyte. Quantitative PCR and western blot were used to measure gene and protein expression of DNMT as well as the activity of DNMTs. Treatment with NGF was found to upregulate both gene and protein expressions as well as total activity of DNMTs in differentiating HT22 cells. Compared to undifferentiating cells, the percentage of differentiating cells at S phase increased significantly when incubated with NGF. In undifferentiated cells, NGF failed to induce gene and protein expressions and activity of DNMTs. Data demonstrate that differentiation of HT22 cells by exposure to NGF involve the activation of DNMTs pathway.

DOI 10.1080/15287394.2018.1504384
2018 Wu P, Yan X-S, Zhang Y, Huo D-S, Song W, Fang X, et al., 'The protective mechanism underlying total flavones of Dracocephalum (TFD) effects on rat cerebral ischemia reperfusion injury.', J Toxicol Environ Health A, 81 1108-1115 (2018)
DOI 10.1080/15287394.2018.1503073
2018 Jia J-X, Yan X-S, Song W, Fang X, Cai Z-P, Huo D-S, et al., 'The protective mechanism underlying phenylethanoid glycosides (PHG) actions on synaptic plasticity in rat Alzheimer's disease model induced by beta amyloid 1-42.', J Toxicol Environ Health A, 81 1098-1107 (2018)
DOI 10.1080/15287394.2018.1501861
2018 Wu P, Yan X-S, Zhang Y, Huo D-S, Song W, Fang X, et al., 'The protective mechanism underlying total flavones of Dracocephalum (TFD) effects on rat cerebral ischemia reperfusion injury.', J Toxicol Environ Health A, 81 1199-1206 (2018)
DOI 10.1080/15287394.2018.1504385
2017 Zhao ZY, Gao YY, Gao L, Zhang M, Wang H, Zhang CH, 'Protective effects of bellidifolin in hypoxia-induced in pheochromocytoma cells (PC12) and underlying mechanisms', Journal of Toxicology and Environmental Health - Part A: Current Issues, 80 1187-1192 (2017) [C1]

© 2017 Taylor & Francis. Bellidifolin, a xanthone compound derived from plants of Gentiana species, is known to exert a variety of pharmacological activities including anti-... [more]

© 2017 Taylor & Francis. Bellidifolin, a xanthone compound derived from plants of Gentiana species, is known to exert a variety of pharmacological activities including anti-oxidation, anti-inflammatory and antitumor actions as well as a protective effect on cerebral ischemic nerve injury. The aim of this study was to examine the protective effects of bellidifolin on nerve injury produced by hypoxia and possible underlying mechanisms using pheochromocytoma cells (PC12). Data showed that the viability of PC12 cells subjected to hypoxia resulted in a significant decrease; however; pretreatment with certain concentrations of bellidifolin (20 or 40¿µmol/L) prior to hypoxia significantly increased the survival rate. The results of immunohistochemical staining analysis revealed that there were no marked alterations in the expression of pERK protein between all bellidifolin groups while the expression of p-p38MAPK protein was significantly enhanced by hypoxia. Pretreatment with different concentrations of bellidifolin followed by hypoxia significantly decreased the expression of p-p38MAPK protein. The results of western blot analysis showed that hypoxia induced the expression of the MAPK signaling pathway downstream of the key apoptosis factor caspase-3. Compared to hypoxia, the expression of caspase-3 in the presence of belliidifolin was significantly lower. Data suggest that bellidifolin may contribute to the protective effects associated with nerve injury initiated by hypoxia by mechanisms related to inhibition of cell apoptosis independent of the ERK pathway, but may involve blockade of p38MAPK signaling pathway activation and downstream caspase-3 expression.

DOI 10.1080/15287394.2017.1367114
Citations Scopus - 2Web of Science - 2
2017 Jia JX, Yan XS, Cai ZP, Song W, Huo DS, Zhang BF, et al., 'The effects of phenylethanoid glycosides, derived from Herba cistanche, on cognitive deficits and antioxidant activities in male SAMP8 mice', Journal of Toxicology and Environmental Health - Part A: Current Issues, 80 1180-1186 (2017) [C1]

© 2017 Taylor & Francis. Cognitive deficits are closely associated with hippocampal synaptic changes. Phenylethanoid glycosides (PhG), derived from Herba cistanche, are know... [more]

© 2017 Taylor & Francis. Cognitive deficits are closely associated with hippocampal synaptic changes. Phenylethanoid glycosides (PhG), derived from Herba cistanche, are known to exert protective effects on cognitive deficits in Alzheimer¿s disease (AD); however, the underlying mechanisms of this herbal extract on cognitive performance remain unclear. The aim of this study was thus to examine the protective mechanism attributed to PhG on cognitive deficits in an AD senescence accelerated mouse prone 8 (SAMP8) model. Cognitive deficit parameters examined included (1) Morris water maze (MWM) assessing cognitive performance and (2) quantification of dendritic spine density in hippocampal CA1 region by Golgi staining, a molecular biomarker of synaptic function. In addition, levels of malondialdehyde (MDA) and activities of superoxide dismutase (SOD) and gluthathione peroxidase (GSH-Px) were determined to examine the potential role of oxidant processes in cognitive dysfunction. Data showed that PhG significantly decreased escape latency and path length, associated with a rise in the percentage of time spent in the target quadrant and number of platform crossings. In addition, PhG significantly increased dendritic spine density in the hippocampal CA1 region accompanied by elevated expression levels of synaptophysin (SYN) and post synaptic density 95 (PSD-95), reduced MDA content, and elevated the activities of SOD and GSH-Px. Data suggest that the ability of PhG to ameliorate cognitive deficits in SAMP8 mice may be related to promotion in synaptic plasticity involving antioxidant processes.

DOI 10.1080/15287394.2017.1367097
Citations Scopus - 2Web of Science - 2
2017 Zhang M, Zheng HX, Gao YY, Zheng B, Liu JP, Wang H, et al., 'The influence of Schisandrin B on a model of Alzheimer¿s disease using ß-amyloid protein Aß

© 2017 Taylor & Francis. Schisandrin B, an active substance, is derived from Chinese herb fruit Wuweizi, which exerts various pharmacological activities and has displayed si... [more]

© 2017 Taylor & Francis. Schisandrin B, an active substance, is derived from Chinese herb fruit Wuweizi, which exerts various pharmacological activities and has displayed significant beneficial effects in ameliorating Alzheimer¿s disease (AD). The aim of this study was to further extend our examination for the use of schisandrin B extract in the potential treatment of AD effects by investigating DNA methylation (DNMT), known to be modified in this disease using SH-SY5Y neuronal cell line exposed to ß-amyloid protein (Aß1-42). In particular, the purpose of this investigation was to examine alterations in mRNA and protein expression of DNMT. Data demonstrated that schisandrin B blocked Aß1-42-mediated injury in SH-SY5Y neuronal cell line as evidenced by a restoration of cellular morphology and cell viability to approximate control levels at the highest 10¿µg/ml Schisandrin B. Incubation with Aß1-42significantly decreased mRNA and protein expression of DNMT3A and DNMT1 in SH-SY5Y neuronal cell line. Incubation with Aß1-42followed by 24 treatment with schisandrin B significantly inhibited the Aß1-42-induced changes in mRNA and protein expression of DNMT3A and DNMT3B in a concentration-dependent manner. It is of interest that the mRNA expression of DNMT3A and DNMT1 were significantly higher than control. Data thus indicate schisandrin B was effective in inhibiting the actions of Aß1-42on cell survival and morphology and that DNA methylation may be associated with the beneficial findings.

DOI 10.1080/15287394.2017.1367133
Citations Scopus - 3Web of Science - 3
2017 Jia JX, Zhang Y, Wang ZL, Yan XS, Jin M, Huo DS, et al., 'The inhibitory effects of Dracocephalum moldavica L. (DML) on rat cerebral ischemia reperfusion injury', Journal of Toxicology and Environmental Health - Part A: Current Issues, 80 1206-1211 (2017) [C1]

© 2017 Taylor & Francis. Ischemia reperfusion injury (IRI) is closely associated with oxidative stress and inflammatory responses. Dracocephalum moldavica L. (DML), a Chines... [more]

© 2017 Taylor & Francis. Ischemia reperfusion injury (IRI) is closely associated with oxidative stress and inflammatory responses. Dracocephalum moldavica L. (DML), a Chinese herbal medicine is known to exert protective effects on myocardial ischemia reperfusion injury in rats by inhibiting oxidation damage and inflammatory reactions. However, the effectiveness of DML in cerebral ischemia reperfusion injury (CIRI) as a protective substance and the underlying mechanisms remain to be determined. The aim of this study was thus to examine the influence of DML on CIRI using a rat model induced by 2-h transient middle cerebral artery occlusion (MCAO) produced by intraluminal suture blockade followed by 22¿h reperfusion. The parameters determined include neurological behavior, histochemical assessment of cerebral infarct volume, and determination of various metabolic biomarkers. Data showed that DML markedly improved neurobehavioral scores and reduced cerebral edema and infarction. In addition, DML significantly reduced malondialdehyde (MDA) content and elevated activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px), in addition, marked decrease in levels of interleukin-6 (IL-6), interleukin-8 (IL-8), and tumor necrosis factor-a (TNF-a). Data suggest that the protective effects of DML on CIRI may be related to processes involving antioxidation and anti-inflammation.

DOI 10.1080/15287394.2017.1367139
Citations Scopus - 2Web of Science - 2
2017 Mu Y, Wang R, Wang H, 'Programmed Cell Death of Cultured A549 Lung Ep-ithelial Cells Induced by Sodium Ar-senite Exposure.', Journal of Medicinal Chemistry and Toxicology, 2 85-89 (2017) [C1]
DOI 10.15436/2575-808X.17.1593
2017 Yu S, Bohatko-Naismith J, Zhang X, Zhou X, Wang P, Wang H, 'Cellular responses in titanium dioxide nanoparticle cytotoxicity studies: parts of the map waiting to be composed', Journal of Medicinal Chemistry and Toxicology, 2 1-9 (2017) [C1]
DOI 10.15436/2575-808X.17.1402
Co-authors Joanna Bohatko-Naismith, Xu Zhang
2017 Zheng Q-N, Wang J, Zhou H-B, Niu S-F, Liu Q-L, Yang Z-J, et al., 'Effectiveness of Amygdalus mongolica oil in hyperlipidemic rats and underlying antioxidant processes.', Journal of toxicology and environmental health. Part A, 80 1193-1198 (2017) [C1]
DOI 10.1080/15287394.2017.1367124
Citations Scopus - 1Web of Science - 1
2017 Liu YZ, Zhang L, Roy-Engel AM, Saito S, Lasky JA, Wang G, Wang H, 'Carcinogenic effects of oil dispersants: A KEGG pathway-based RNA-seq study of human airway epithelial cells', Gene, 602 16-23 (2017) [C1]

© 2016 Elsevier B.V. The health impacts of the BP oil spill are yet to be further revealed as the toxicological effects of oil products and dispersants on human respiratory system... [more]

© 2016 Elsevier B.V. The health impacts of the BP oil spill are yet to be further revealed as the toxicological effects of oil products and dispersants on human respiratory system may be latent and complex, and hence difficult to study and follow up. Here we performed RNA-seq analyses of a system of human airway epithelial cells treated with the BP crude oil and/or dispersants Corexit 9500 and Corexit 9527 that were used to help break up the oil spill. Based on the RNA-seq data, we then systemically analyzed the transcriptomic perturbations of the cells at the KEGG pathway level using two pathway-based analysis tools, GAGE (generally applicable gene set enrichment) and GSNCA (Gene Sets Net Correlations Analysis). Our results suggested a pattern of change towards carcinogenesis for the treated cells marked by upregulation of ribosomal biosynthesis (hsa03008) (p¿=¿1.97E¿-¿13), protein processing (hsa04141) (p¿=¿4.09E¿-¿7), Wnt signaling (hsa04310) (p¿=¿6.76E¿-¿3), neurotrophin signaling (hsa04722) (p¿=¿7.73E¿-¿3) and insulin signaling (hsa04910) (p¿=¿1.16E¿-¿2) pathways under the dispersant Corexit 9527 treatment, as identified by GAGE analysis. Furthermore, through GSNCA analysis, we identified gene co-expression changes for several KEGG cancer pathways, including small cell lung cancer pathway (hsa05222, p¿=¿9.99E¿-¿5), under various treatments of oil/dispersant, especially the mixture of oil and Corexit 9527. Overall, our results suggested carcinogenic effects of dispersants (in particular Corexit 9527) and their mixtures with the BP crude oil, and provided further support for more stringent safety precautions and regulations for operations involving long-term respiratory exposure to oil and dispersants.

DOI 10.1016/j.gene.2016.11.028
2016 Liu Y, Li Y, Yang T, Yang J, Wang H, Wu G, 'Acute changes in murine hippocampus and olfactory bulb after nasal instillation of varying size cerium dioxide particles', JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A-CURRENT ISSUES, 79 869-877 (2016) [C1]
DOI 10.1080/15287394.2016.1193116
2016 Huo DS, Sun JF, Zhang B, Yan XS, Wang H, Jia JX, Yang ZJ, 'Protective effects of testosterone on cognitive dysfunction in Alzheimer¿s disease model rats induced by oligomeric beta amyloid peptide 1-42', Journal of Toxicology and Environmental Health - Part A: Current Issues, 79 856-863 (2016) [C1]

© 2016 Taylor & Francis. Cognitive dysfunction is known to be influenced by circulating sex steroidal hormones. The aim of this study was to examine the protective effect an... [more]

© 2016 Taylor & Francis. Cognitive dysfunction is known to be influenced by circulating sex steroidal hormones. The aim of this study was to examine the protective effect and possible protective mechanism of testosterone (T) on cognitive performance in male rats induced by intrahippocampal injections of beta amyloid 1-42 oligomers (Aß1-42). Treatment with T as evidenced by the Morris water maze (MWM) test significantly shortened escape latency and reduced path length to reach the platform compared to the control (C). During probe trials, the T group displayed a significantly greater percent of time in the target quadrant and improved the number of platform crossings compared with C, flutamide (F), an antiandrogen, and a combined F and T group. Flutamide markedly inhibited the influence of T on cognitive performance. Following Nissl staining, the number of intact pyramidal cells was significantly elevated in the T group, and the effect of T was blocked by F. Immunohistochemisty and Western blot analysis showed that the protein expression level of Aß 1-42 was markedly decreased and expression levels of synaptophysin (SYN) significantly increased with T, while F inhibited all T-mediated effects. Our data suggest that the influence of T on cognitive performance was mediated via androgen receptors (AR) to remove beta amyloid, which leads to enhanced synaptic plasticity.

DOI 10.1080/15287394.2016.1193114
Citations Scopus - 6Web of Science - 6
2016 Jia JX, Cui CL, Yan XS, Zhang BF, Song W, Huo DS, et al., 'Effects of testosterone on synaptic plasticity mediated by androgen receptors in male SAMP8 mice', Journal of Toxicology and Environmental Health - Part A: Current Issues, 79 849-855 (2016) [C1]

© 2016 Taylor & Francis. Synaptic changes are closely associated with cognitive deficits. In addition, testosterone (T) is known to exert regulative effects on synaptic plas... [more]

© 2016 Taylor & Francis. Synaptic changes are closely associated with cognitive deficits. In addition, testosterone (T) is known to exert regulative effects on synaptic plasticity. T may improve cognitive deficits in Alzheimer¿s disease (AD) patients, but the underlying mechanisms of androgenic action on cognitive performance remain unclear. The aim of this study was thus to examine the protective mechanism attributed to T on cognitive performance in an AD senescence, accelerated mouse prone 8 (SAMP8) animal model. Using Golgi staining to quantify the dendritic spine density in hippocampal CA1 region, molecular biomarkers of synapse function were analyzed using immunohistochemistry and western blot. T significantly increased the dendritic spine density in hippocampal CA1 region, while flutamide (F) inhibited these T-mediated effects. Immunohistochemistry and western blot analysis showed that the expression levels of brain derived neurotrophic factor (BDNF), postsynaptic density 95 (PSD-95), and p-cyclic-AMP response element binding protein (CREB)/CREB levels were significantly elevated in the T group, but F reduced the T-induced effects in these biomarkers to control levels. There were no significant differences in the expression levels of PSD-95, BDNF, and p-CREB/CREB between C and F. These findings indicate that the effects of T on improvement in synaptic plasticity were mediated via androgen receptor (AR). It is conceivable that new treatments targeted toward preventing synaptic pathology in AD may involve the use of androgen-acting drugs.

DOI 10.1080/15287394.2016.1193113
Citations Scopus - 12Web of Science - 11
2016 Major D, Derbes RS, Wang H, Roy-Engel AM, 'Effects of corexit oil dispersants and the WAF of dispersed oil on DNA damage and repair in cultured human bronchial airway cells, BEAS-2B', Gene Reports, 3 22-30 (2016) [C1]

© 2016 Elsevier Inc. All rights reserved. Large quantities of dispersants were used as a method to disperse the roughly 210 million gallons of spilled crude oil that consumed the ... [more]

© 2016 Elsevier Inc. All rights reserved. Large quantities of dispersants were used as a method to disperse the roughly 210 million gallons of spilled crude oil that consumed the Gulf of Mexico. Little is known if the oil-dispersant and oil-dispersant mixtures on human airway BEAS-2B epithelial cells. Here we present the cytotoxic and genotoxic in vitro effects on the human lung cells BEAS-2B following exposure to and oil-dispersant mixtures on human airway BEAS-2B epithelial cells. Here we present the cytotoxic and genotoxic in vitro effects on the human lung cells BEAS-2B following exposure to Corexit dispersants EC9500 and EC9527, Water Accommodated Fraction (WAF) -crude, WAF-9500 + Oil, and WAF-9527 + Oil. Cellular cytotoxicity to WAF-dispersed oil samples was observed at concentrations greater than 1000 ppm with over 70% of observed cellular death. At low concentration exposures (100 and 300 ppm) DNA damage was evidenced by the detection of single strand breaks (SSBs) and double strand breaks (DSBs) as measured by alkaline and neutral comet assay analyses. Immunoblot analyses of the phosphorylated histone H2A.X (¿-H2A.X) and tumor suppressor p53 protein confirmed activation of the DNA damage response due to the exposure-induced DNA breaks. Although, many xenobiotics interfere with DNA repair pathways, in vitro evaluation of the nucleotide excision repair (NER) and DSB repair pathways appear to be unaffected by the oil-dispersant mixtures tested. Overall, this study supports that oil-dispersant mixtures induce genotoxic effects in culture.

DOI 10.1016/j.genrep.2015.12.002
Citations Scopus - 2Web of Science - 2
2016 Liu Y-Z, Roy-Engel AM, Baddoo MC, Flemington EK, Wang G, Wang H, 'The impact of oil spill to lung health--Insights from an RNA-seq study of human airway epithelial cells.', Gene, 578 38-51 (2016) [C1]
DOI 10.1016/j.gene.2015.12.016
Citations Scopus - 3Web of Science - 4
2015 Sun Y, Shao H, Wang H, 'Occupational diseases prevention and control in China: a comparison with the United States', Journal of Public Health (Germany), 23 379-386 (2015)

© 2015, Springer-Verlag Berlin Heidelberg. Aim: Occupational diseases have become an important health issue and social problem for countries throughout the world. The United State... [more]

© 2015, Springer-Verlag Berlin Heidelberg. Aim: Occupational diseases have become an important health issue and social problem for countries throughout the world. The United States and China face the serious situation of occupational diseases prevention and control. Methods: This paper compared the prevention and control of occupational diseases in the United States and China in the following aspects: status, law and related agencies, definition and categories, surveillance and reporting systems, diagnosis and identification. Results: China faces more challenges in occupational diseases prevention and control. Conclusion: There is a gap in the overall strength of occupational disease prevention and control between the US and China. There is a lot of work to do for China.

DOI 10.1007/s10389-015-0689-0
Citations Scopus - 1Web of Science - 1
2015 Zhang M, Huo DS, Cai ZP, Shao G, Wang H, Zhao ZY, Yang ZJ, 'The Effect of Schizandrol A-Induced DNA Methylation on SH-SY5YAB 1-40 Altered Neuronal Cell Line: A Potential Use in Alzheimers Disease', Journal of Toxicology and Environmental Health - Part A: Current Issues, 78 1321-1327 (2015) [C1]

Copyright © Taylor & Francis Group, LLC. Disturbances in DNA methylation are postulated to result in various central nervous system diseases including Alzheimers disease (AD... [more]

Copyright © Taylor & Francis Group, LLC. Disturbances in DNA methylation are postulated to result in various central nervous system diseases including Alzheimers disease (AD). The SH-SY5Y neuronal cell line treated with Aß1-40 (5 mol/L) protein is considered to be a model of AD. Hence the aim of this study was to examine the influence of Schizandrol A, a plant extract, on DNA methylation in SH-SY5Y cells exposed to Aß1-40. Aß1-40 were incubated with varying concentrations of Sehizandrol A to a final concentration of 1 (low), 3 (intermediate) or 9 g/ml (high). Exposure of SH-SY5Y with Aß1-40 reduced viability, and altered cellular morphology and mRNA expression of DNA methyltransferase (DNMT3A) and DNMT3B. Treatment with 1 or 3 g/ml Sehizandrol A resulted in normal cell morphology as well as elevated cell number, enhanced viability, and increased mRNA expression of DNMT3A and DNMT3B compared to saline. However, an increase in Sehizandrol A to 9 g/ml produced a fall in cell viability, as well as a decrease in mRNA DNMT3A and DNMT3B expression to control levels. Data demonstrated that Schizandrol A at 1 or 3 g/ml improved cell morphological appearance and viability of Aß1-40 injured SH-SY5Y cells by an enhanced DNA methylation pathway.

DOI 10.1080/15287394.2015.1085942
Citations Scopus - 5Web of Science - 2
2015 Jia JX, Cui CL, Song W, Yan XS, Huo DS, Wang H, Yang ZJ, 'Effects of Testosterone Treatment on Synaptic Plasticity and Behavior in Senescence Accelerated Mice', Journal of Toxicology and Environmental Health - Part A: Current Issues, 78 1311-1320 (2015) [C1]

Copyright © Taylor & Francis Group, LLC. Learning and memory are known to be influenced by circulating sex steroidal hormones and these behavioral processes are diminished i... [more]

Copyright © Taylor & Francis Group, LLC. Learning and memory are known to be influenced by circulating sex steroidal hormones and these behavioral processes are diminished in aging. Thus, the aim of this study was to examine the mechanism underlying testosterone-induced effects on cognitive performance in the senescence accelerated mouse P8 (SAMP8) model. Treatment with testosterone (T) as evidenced by the Morris water maze test produced a significantly shorter escape latency and reduced path length to reach the platform compared to the control (C). No significant differences were noted in mean swim speed among all groups. During the probe trials, the T group spent a significantly greater percent of time in the target quadrant and improved the number of platform crossings. Flutamide (F), an antiandrogen, significantly inhibited the effects of T on behavioral and memory performances indicators. Following Nissl staining, the number of intact pyramidal cells was markedly elevated in the treated mice, and this effect was blocked by F. Immunohistochemistry and Western blot analysis showed that the expression levels of NMDAR1, SYN, and p-CREC/CREB protein levels were significantly increased in the T group, while F inhibited the T-mediated effects. Western blot analysis showed that there were no significant differences in the expression levels of SYN, p-CREC/CREB, and NMDAR1 between C, F, and F + T groups. Reverse-transcription polymerase chain reaction (RT-PCR) analysis showed that the mRNA expression levels of NMDAR1 and SYN were significantly increased in T-administered mice, while F inhibited the T-mediated effects. Data suggest that the T-mediated increase in SYN expression levels resulted in improvement in behavioral performances and learning, which may involve stimulation of central nervous system androgen receptors (AR).

DOI 10.1080/15287394.2015.1085839
Citations Scopus - 7Web of Science - 5
2015 Huo DS, Zhang M, Cai ZP, Dong CX, Wang H, Yang ZJ, 'The Role of Nerve Growth Factor in Ginsenoside Rg1-Induced Regeneration of Injured Rat Sciatic Nerve', Journal of Toxicology and Environmental Health - Part A: Current Issues, 78 1328-1337 (2015) [C1]

Copyright © Taylor & Francis Group, LLC. Sciatic nerve injury is commonly seen in clinical practice predominantly associated with trauma or sports injuries. Recent studies i... [more]

Copyright © Taylor & Francis Group, LLC. Sciatic nerve injury is commonly seen in clinical practice predominantly associated with trauma or sports injuries. Recent studies indicated that ginsenoside Rg1 (Gs Rg1), extracted from Chinese herbs, was found to promote regeneration of injured rat sciatic nerve and that nerve growth factor (NGF) may be involved in this process. The aim of this study was to examine the role that NGF may play in ginsenoside Rg1-induced regeneration of rat sciatic nerve following injury. Animals following surgical right sciatic nerve injury were subsequently administered intraperitoneally either saline (sham control) or different doses of 2, 4, 8, or 12 mg/kg daily GsRg1 for 2 to 8 wk. In addition, 100 g/kg mecobalamin, a drug utilized to treat nerve injuries, was employed as a positive control. After 2, 4, or 8 wk, sciatic functional index (SFI) and mean nerve conduction velocity (MNCV), markers of sciatic nerve function, were assessed to determine whether recovery of injured sciatic nerve occurred. In addition, immunohistochemistry and Western blot methods were used to examine NGF protein expression changes. Results showed that all doses of GsRg1 significantly increased SFI and MNCV in injured sciatic-nerve-damaged rats in a manner similar to that noted with mecobalamin. It is of interest that the intermediate 4- and 8-mg/kg doses were more effective in restoring nerve functions. Immunohistochemistry and Western blot results also demonstrated a similar pattern with enhanced NGF protein expression at all doses, but greater effects were noted at 4 and 8 mg/kg GsRg1. Data suggest that GsRg1 promotes recovery of injured sciatic nerve functions within a specific dose range and that NGF may be involved in this physiological process.

DOI 10.1080/15287394.2015.1085943
Citations Scopus - 1Web of Science - 1
2015 Wang C, Leigh J, Wang S, Wang H, 'Intervention of pentoxifylline on silica-induced inflammatory reaction and apoptosis', TOXICOLOGICAL AND ENVIRONMENTAL CHEMISTRY, 97 640-650 (2015) [C1]
DOI 10.1080/02772248.2015.1054609
2014 Sun Y, Xu L, Shao H, Wang H, 'China's laws, rights, and administrative structures in occupational safety and health: A comparison with the United States', JOURNAL OF PUBLIC HEALTH POLICY, 35 455-469 (2014)
DOI 10.1057/jphp.2014.12
2013 Yadav S, Anbalagan M, Shi Y, Wang F, Wang H, 'Arsenic inhibits the adipogenic differentiation of mesenchymal stem cells by down-regulating peroxisome proliferator-activated receptor gamma and CCAAT enhancer-binding proteins', TOXICOLOGY IN VITRO, 27 211-219 (2013)
DOI 10.1016/j.tiv.2012.10.012
Citations Scopus - 12Web of Science - 8
2013 Shi Y, Roy-Engel AM, Wang H, 'Effects of Corexit Dispersants on Cytotoxicity Parameters in a Cultured Human Bronchial Airway Cells, BEAS-2B', JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A-CURRENT ISSUES, 76 827-835 (2013)
DOI 10.1080/15287394.2013.821396
Citations Scopus - 14Web of Science - 14
2013 Wang H, Wang JJ, Sanderson BJS, 'IN VITRO ADVERSE EFFECTS OF IRON ORE DUSTS ON HUMAN LYMPHOBLASTOID CELLS IN CULTURE', JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A-CURRENT ISSUES, 76 874-882 (2013)
DOI 10.1080/15287394.2013.826566
2012 Major DN, Wang H, 'How public health impact is addressed: a retrospective view on three different oil spills', TOXICOLOGICAL AND ENVIRONMENTAL CHEMISTRY, 94 442-467 (2012)
DOI 10.1080/02772248.2012.654633
Citations Scopus - 7Web of Science - 7
2012 Major D, Zhang Q, Wang G, Wang H, 'Oil-dispersant mixtures: understanding chemical composition and its relation to human toxicity', TOXICOLOGICAL AND ENVIRONMENTAL CHEMISTRY, 94 1832-1845 (2012)
DOI 10.1080/02772248.2012.730202
Citations Scopus - 11Web of Science - 10
2012 Wang H, Shi Y, Major D, Yang Z, 'Lung epithelial cell death induced by oil-dispersant mixtures', TOXICOLOGY IN VITRO, 26 746-751 (2012)
DOI 10.1016/j.tiv.2012.03.011
Citations Scopus - 9Web of Science - 10
2011 Yang ZJ, Song F, Wang ZJ, Shi Y, Fang G, Wang H, 'Co-administration of Mixed Steroid Hormones can Enhance the Recovery of Spermatogenesis Damaged by Gossypol Acetic Acid in Adult Rats', Journal of Reproduction and Contraception, 22 233-245 (2011)

Objective: To determine whether co-administration of mixed steriod hormones can enhance the restoration of spermatogenesis damaged by gossypol acetic acid (GA). Methods: Adult mal... [more]

Objective: To determine whether co-administration of mixed steriod hormones can enhance the restoration of spermatogenesis damaged by gossypol acetic acid (GA). Methods: Adult male Wistar rats were treated daily for 8 weeks with GA at 50mg/kg plus testosterone undercanoate (100 mg/kg)/desogestrel (0.125 mg/kg)/mini-dose ethinylestradiol (0.025 mg/kg) (TU/DSG/EE), followed by a period of 9 weeks for recovery. Control animals were administered the same dose of GA or TU/DSG/EE, and vehicle, respectively. Testis weight, testicular sperm head count and histological analysis were utilized to assess the spermatogenesis. Results: At the end of the 9-week reovery period, in rats given GA alone, spermatogenesis steadily declined. However, when rats received combined hormone administration during GA treatment, this decline was prevented and an complete recovery of spermagenesis occurred. The haploid spermatids and spermatocytes was not to be protected but to be more aggravatedly damaged. The excellent recovery must have resulted from that the hormone treatment could protect the ability of stem spermatogonia to differentialte and evolve progressively into mature spermatozoa. In addition, the concentrations of serum LH, FSH and intratesticular testosteron (ITT) notably decreased after 2 or 8 weeks of treatment, then returned to control levels at the end of 9-week recovery period. Conclusion: Steriod hormone treatment enhaces the recovery of spermatogenesis through preventing seminiferous epithelim from GA-induced destructive damage in rats. The enhanced recovery was closely associated with the marked suppression in intratesticular testosteron (ITT). © 2011 The Editorial Board of Journal of Reproduction and Contraception.

DOI 10.1016/S1001-7844(12)60020-0
Citations Scopus - 1
2010 Yadav S, Shi Y, Wang F, Wang H, 'Arsenite induces apoptosis in human mesenchymal stem cells by altering Bc1-2 family proteins and by activating intrinsic pathway', TOXICOLOGY AND APPLIED PHARMACOLOGY, 244 263-272 (2010)
DOI 10.1016/j.taap.2010.01.001
Citations Scopus - 27Web of Science - 27
2010 Wang F, Shi Y, Yadav S, Wang H, 'p52-Bcl3 complex promotes cyclin D1 expression in BEAS-2B cells in response to low concentration arsenite', TOXICOLOGY, 273 12-18 (2010)
DOI 10.1016/j.tox.2010.04.009
Citations Scopus - 10Web of Science - 10
2010 Shi Y, Wang F, He J, Yadav S, Wang H, 'Titanium dioxide nanoparticles cause apoptosis in BEAS-2B cells through the caspase 8/t-Bid-independent mitochondrial pathway', TOXICOLOGY LETTERS, 196 21-27 (2010)
DOI 10.1016/j.toxlet.2010.03.014
Citations Scopus - 59Web of Science - 50
2010 Shi Y, Yadav S, Wang F, Wang H, 'Endotoxin Promotes Adverse Effects of Amorphous Silica Nanoparticles on Lung Epithelial Cells in Vitro', JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A-CURRENT ISSUES, 73 748-756 (2010)
DOI 10.1080/15287391003614042
Citations Scopus - 41Web of Science - 39
2010 Yadav S, Shi Y, Wang H, 'IL-16 effects on A549 lung epithelial cells: Dependence on CD9 as an IL-16 receptor?', JOURNAL OF IMMUNOTOXICOLOGY, 7 183-193 (2010)
DOI 10.3109/15476911003649346
Citations Scopus - 2Web of Science - 4
2007 Lawson G, Wang H, 'Public Health Implications of Asbestos: Epidemiological Aspects', Environmental Health, 7 22-27 (2007)
2007 Lawson G, Wang H, 'Harmful Effects of Asbestos: Mechanisms of Toxicity', Environmental Health, 7 28-35 (2007)
2007 Wang JJ, Sanderson BJS, Wang H, 'Cytotoxicity and genotoxicity of ultrafine crystalline SiO2 particulate in cultured human lymphoblastoid cells', ENVIRONMENTAL AND MOLECULAR MUTAGENESIS, 48 151-157 (2007)
DOI 10.1002/em.20287
Citations Scopus - 86Web of Science - 83
2007 Wang JJ, Sanderson BJS, Wang H, 'Cyto- and genotoxicity of ultrafine TiO2 particles in cultured human lymphoblastoid cells', MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS, 628 99-106 (2007)
DOI 10.1016/j.mrgentox.2006.12.003
Citations Scopus - 374Web of Science - 336
2007 Sanderson BJS, Wang JJ, Wang H, 'Letter and new data in response to Letter to the Editor by William P. Gulledge about article "Cyto- and genotoxicity of ultrafine TiO2 particles in cultured human lymphoblastoid cells" by Wang et al. Reply', MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS, 634 243-244 (2007)
DOI 10.1016/j.mrgentox.2007.06.007
Citations Scopus - 1Web of Science - 1
2007 Wang JJ, Wang H, Sanderson BJS, 'Ultrafine quartz-induced damage in human lymphoblastoid cells in vitro using three genetic damage end-points', TOXICOLOGY MECHANISMS AND METHODS, 17 223-232 (2007)
DOI 10.1080/15376510600943775
Citations Scopus - 18Web of Science - 15
2006 Wang H, Leigh J, 'Effects of nitric oxide synthase inhibitor ¿-Nitro-L-Arginine Methyl Ester, on silica-induced inflammatory reaction and apoptosis', Particle and Fibre Toxicology, 3 (2006)

Background: Although nitric oxide is overproduced by macrophages and neutrophils after exposure to silica, its role in silica-induced inflammatory reaction and apoptosis needs fur... [more]

Background: Although nitric oxide is overproduced by macrophages and neutrophils after exposure to silica, its role in silica-induced inflammatory reaction and apoptosis needs further clarification. In this study, rats were intratracheally instilled with either silica suspension or saline to examine inflammatory reactions and intraperitoneally injected with ¿-nitro-L-arginine methyl ester (L-NAME), an inhibitor of nitric oxide synthases, or saline to examine the possible role of nitric oxide production in the reaction. Results: Results showed that silica instillation induced a strong inflammatory reaction indicated by increased total cell number, number of neutrophils, protein concentration and lactate dehydrogenase (LDH) activity in bronchoalveolar lavage fluid (BALF). There were no significant differences in these indices between silica-instilled groups with and without L-NAME injection (p > 0.05) except LDH level. The results also showed that apoptotic leucocytes were identified in BALF cells of silica-instilled groups whereas no significant difference was found between silica-instilled groups with and without L-NAME injection in the apoptotic reaction (p > 0.05). Silica instillation significantly increased the level of BALF nitrite/nitrate and L-NAME injection reduced this increase. Conclusion: Intratracheal instillation of silica caused an obvious inflammatory reaction and leucocyte apoptosis, but these reactions were not influenced by intraperitoneal injection of L-NAME and reduced production of NO. This supports the possibility that silica-induced lung inflammation and BALF cell apoptosis are via NO-independent mechanisms. © 2006 Wang and Leigh; licensee BioMed Central Ltd.

DOI 10.1186/1743-8977-3-14
Citations Scopus - 5
2006 Loughran-Fowlds A, Oei J, Wang H, Xu H, Wimalasundera N, Egan C, et al., 'The influence of gestation and mechanical ventilation on serum Clara cell secretory protein (CC10) concentrations in ventilated and nonventilated newborn infants', PEDIATRIC RESEARCH, 60 103-108 (2006)
DOI 10.1203/01.pdr.0000219388.56608.77
Citations Scopus - 13Web of Science - 13
2006 Wang H, Peng X, 'Nitric Oxide: A Non-invasive Measure of Silica Induced Health Effects and Its Potential Role in Silica Induced Effects', Environmental Health, 6 17-23 (2006)
2006 Wang H, Pisaniello D, 'The Role of Nitric Oxide or Its Metabolites in the Development of Asbestos Induced Mesothelioma', Environmental Health, 6 24-30 (2006)
2006 Lawson G, Wang H, 'Public Health Impact of Diesel Exhaust: Toxicity of Nano-sized Diesel Exhaust Particles - Part III', Environmental Health, 6 28-33 (2006)
2006 Lawson G, Wang H, Peng X, 'Animal Model of Silicosis and Silica-induced Inflammation', Environmental Health, 6 25-31 (2006)
2006 Wang H, Peng X, Lawson G, 'Silica Carcinogenesis and the Possibility to Assess the Carcinogenesis by Micronucleus Formation in Alveolar Macrophages ex vivo', Environmental Health, 6 32-38 (2006)
2006 Banerjee KK, Wang H, Pisaniello D, 'Iron-ore Dust and Its Health Impacts', Environmental Health, 6 11-16 (2006)
2006 Lawson G, Wang H, 'Public Health Impact of Diesel Exhaust: Toxicity of Nano-sized Diesel Exhaust Particles - Part I', Environmental Health, 6 17-21 (2006)
2006 Lawson G, Wang H, 'Public Health Impact of Diesel Exhaust: Toxicity of Nano-sized Diesel Exhaust Particles - Part II', Environmental Health, 6 22-27 (2006)
2005 Thomas PS, Gibson PG, Wang H, Shah S, Henry RL, 'The relationship of exhaled nitric oxide to airway inflammation and responsiveness in children', Journal of Asthma, 42 291-295 (2005) [C1]
DOI 10.1081/JAS-200057908
Citations Scopus - 36Web of Science - 37
Co-authors Peter Gibson
2005 Gu ZH, Wang H, Thomas P, Henry R, Wang R, 'Respiratory Health of Underground Coal Miners in Newcastle', Environmental Health, 5 14-22 (2005)
2005 Wang H, Leigh J, 'Intervention of Ticlopidine on Silica-induced Inflammatory Reaction and Apoptosis', Environmental Health, 5 23-32 (2005)
2003 Gibson PG, Henry RL, Shah S, Powell H, Wang H, 'Migration to a Western Country Increases Asthma Symptoms But Not Eosinophilic Airway Inflammation', Pediatric Pulmonology, 36 209-215 (2003) [C1]
DOI 10.1002/ppul.10323
Citations Scopus - 43Web of Science - 40
Co-authors Peter Gibson
2003 Oei J, Lui K, Wang H, Henry R, 'Decreased neutrophil apoptosis in tracheal fluids of preterm infants at risk of chronic lung disease', ARCHIVES OF DISEASE IN CHILDHOOD, 88 245-249 (2003)
Citations Scopus - 21Web of Science - 15
2002 Dakin CJ, Numa AH, Wang H, Morton JR, Vertzyas CC, Henry RL, 'Inflammation, infection, and pulmonary function in infants and young children with cystic fibrosis', American Journal of Respiratory and Critical Care Medicine, 165 904-910 (2002)

Our aim was to study the effect of lower airway infection on clinical parameters, pulmonary function tests, and inflammation in clinically stable infants and young children with c... [more]

Our aim was to study the effect of lower airway infection on clinical parameters, pulmonary function tests, and inflammation in clinically stable infants and young children with cystic fibrosis (CF). To accomplish this goal, a prospective cohort of screened CF patients under 4 years of age were studied, using elective anesthesia and intubation for: passive respiratory mechanics (single breath occlusion passive deflation) and lung volumes (nitrogen washout), under neuromuscular blockade; and bronchoalveolar lavage (BAL) of 3 main bronchi for cytology, cytokine interleukin (IL)-8, and quantitative microbiology. There were 22 children studied, with a mean age of 23.2 months (6.7-44 months). A greater relative risk of lower airway pathogens was associated with prior respiratory admission (3.60, 95% confidence interval [Cl] 2.87-4.51), history of asthma (1.75, 95% Cl 1.52-2.03), and chronic symptoms (1,50, 95% Cl 1.23-1.83), especially wheeze (1.88, 95% Cl 1.61-2.19). Lower respiratory pathogens (= 10 cfu/ml BAL) were found in 14 out of 22, and greater than 105 cfu/ml in 8 out of 22 subjects. The level of pathogens in BAL (log10 cfu/ml) explained 78% of the variability in percent neutrophils and 34% of the variability in IL-8 levels. Pathogen level also correlated with pulmonary function tests of specific respiratory system compliance (r -0.49, p = 0.02) and functional residual capacity over total lung capacity (r 0.49, p = 0.03). We conclude that the presence of pathogens in the lower airways correlated with levels of inflammation, respiratory system compliance, and degree of air trapping.

DOI 10.1164/ajrccm.165.7.2010139
Citations Scopus - 186
2002 Wang H, Oei J, Lui K, Henry R, 'Interleukin-16 in tracheal aspirate fluids of newborn infants', EARLY HUMAN DEVELOPMENT, 67 79-86 (2002)
DOI 10.1016/S0378-3782(01)00257-2
Citations Scopus - 12Web of Science - 10
2002 Oei J, Lui K, Wang H, Henry R, 'Decreased interleukin-10 in tracheal aspirates from preterm infants developing chronic lung disease', ACTA PAEDIATRICA, 91 1194-1199 (2002)
Citations Scopus - 30Web of Science - 26
2002 Dakin CJ, Numa AH, Wang H, Morton JR, Vertzyas CC, Henry RL, 'Inflammation, infection, and pulmonary function in infants and young children with cystic fibrosis', AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, 165 904-910 (2002)
DOI 10.1164/rccm.2010139
Citations Web of Science - 163
2002 Dakin CJ, Pereira JK, Henry RL, Wang H, Morton JR, 'Relationship between sputum inflammatory markers, lung function, and lung pathology on high-resolution computed tomography in children with cystic fibrosis', PEDIATRIC PULMONOLOGY, 33 475-482 (2002)
DOI 10.1002/ppul.10109
Citations Scopus - 32Web of Science - 24
2001 Wang H, 'Interlel'kin-16 in trachea aspirates of preterm infants with chronic lung disease', Respirology, 6 (2001)

Current views on pathogenesis of chronic lung disease (OLD) support an imbalance of inflammatory and healing processes in susceptible premature infants. Persistence of proinflamma... [more]

Current views on pathogenesis of chronic lung disease (OLD) support an imbalance of inflammatory and healing processes in susceptible premature infants. Persistence of proinflammatory cytokines such as IL-6. IL-8 and TNF-lalpa in trachéal aspirate fluide (TAP) could be a marker of CLD. However, their use in early prediction of Cl.D development is limited. Over the last two decades, systemic dexamethasone have been used in an attempt to attenuate this chronic inflammatory process in CLD infants. Corticosteroids inhibit transcription of some proinflammatory cytokines. It has been demonstrated that dexamethasone can suppress the production of IL-16.11.-16 is a chemoattractant of CD4 cells including lymphocytes and monocytes. Lymphocytes and macrophages transformed from monocytes are more frequently encountered in persistent lung inflammation than acute inflammation. Thus, we hypothesized that IL-16 may play a role in the persistence of lung inflammation in the development of CLD. Fifty-five TAP samples obtained from mechanically ventilated preterm infants during the first 4 weeks of life were analysed by EL1SA kit method (R&D). Among the 17 samples from preterm NON-CLD infants. IL-16 was undetectable. Among the 38 samples from CLD infants, 15 samples were detected (39.5%). The IL-16 range was 900-42100 pg'ml. Considerable proportion of CLD infants ere treated with dexamethasone, both the IL-16 levels and the positive rate may have been affected. Based on the above preliminary result, we concluded that IL-16 is detectable in preterm infants suffered from CLD. Further analysis and studies particularly regarding the influence of corticosteroids treatment are needed.

1999 Dakin C, Wang H, Morton J, Henry R, 'Correlation between sputum cytology in cystic fibrosis and response to treatment of acute exacerbation', Respirology, 4 (1999)

In cystic fibrosis (CF) changes in pulmonary infection or inflammation should be reflected in sputum cytology, with variability between samples being explained by the disease proc... [more]

In cystic fibrosis (CF) changes in pulmonary infection or inflammation should be reflected in sputum cytology, with variability between samples being explained by the disease process. Original sputum processing techniques may fail to show a change due to specimen cell clumping. Method: Prospective study of children with CF admitted to hospital with an exacerbation of chest infection, from July to October 1998. Spontaneous sputum samples were collected during admission. Sputum processing was selective, with dispersion by the 3 enzyme method. Specimens were discarded if >3% squamous cells. Other variables recorded included demographic, day of admission and FEVi. Results: There were 10 children studied (5 males) with mean age 12.1 years (8.1-19.1). Mean admission length was 13 days (4-20). 30 specimens were processed, with >2 values for 5/10 children. FEVi mean at admission was 52% predicted (25-72%), and at discharge 58% (29-93%). Sputum cytology total cell count (TCC) mean was 34.58 x106 (+/- 38.33), with neutrophil mean 33.21 x 10 (+/- 37.77). In 9/10 children the TCC varied inversely with FEVi. In 5/10 the TCC decreased > 2x and FEVi increased >10%, in 3/10 neither TCC nor FEVi changed, and in 1/10 TCC increased and FEVi decreased. Absolute values of FEVi correlated negatively with TCC (-0.54, p=0.01). Conclusion: Absolute values of sputum TCC were useful in reflecting response to treatment during admission. Not every patient had a change in TCC. Using this sputum processing method, there was a significant correlation between sputum TCC and lung function.

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Conference (6 outputs)

Year Citation Altmetrics Link
2018 Holland J, Wang H, James C, 'CAN THE SMARTCAP (R) ALARM RATE PREDICT FATIGUE RELATED MEDICAL CONDITIONS?', INTERNAL MEDICINE JOURNAL (2018)
Co-authors Carole James
2015 Liu X, Pisaniello D, Sanderson B, Wang H, 'CYTOTOXICITY AND GENOTOXICITY OF ULTRAFINE IRON-ORE DUST', RESPIROLOGY, Queensland, AUSTRALIA (2015)
2010 Liu X, Salter A, Thomas P, Leigh J, Wang H, 'EXHALED NITRIC OXIDE LEVELS AND LUNG FUNCTION CHANGES OF UNDERGROUND COAL MINERS IN NEWCASTLE, AUSTRALIA', JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A-CURRENT ISSUES, Cape Town, SOUTH AFRICA (2010)
DOI 10.1080/15287390903486592
Citations Scopus - 2Web of Science - 2
2004 Louhgran-Fowlds AS, Oei J, Wimalasunderera N, Wang H, Xu HX, An C, et al., 'The influence of gestation and mechanical ventilation on serum Clara Cell Protein in preterm infants', PEDIATRIC RESEARCH, San Francisco, CA (2004)
2000 Leigh J, Bonin A, Wang H, 'In vivo genotoxicity of crystalline silica as evidenced by micronuclei in pulmonary alveolar macrophages: Low-dose study', INHALATION TOXICOLOGY, MAASTRICHT, NETHERLANDS (2000)
DOI 10.1080/08958370050165058
Citations Web of Science - 6
1997 Leigh J, Wang H, Bonin A, Peters M, Ruan XY, 'Silica-induced apoptosis in alveolar and granulomatous cells in vivo', ENVIRONMENTAL HEALTH PERSPECTIVES, LAKE PLACID, NY (1997)
DOI 10.2307/3433540
Citations Web of Science - 41
Show 3 more conferences
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Grants and Funding

Summary

Number of grants 1
Total funding $45,500

Click on a grant title below to expand the full details for that specific grant.


20181 grants / $45,500

The Role of Olfactory Receptor in Allergic Inflammation of Respiratory System$45,500

Funding body: Valentine Badham

Funding body Valentine Badham
Project Team Doctor He Wang, Professor Phil Hansbro, Miss Pu Wang
Scheme Valentine Badham PhD Scholarship Project Funds
Role Lead
Funding Start 2018
Funding Finish 2021
GNo G1800956
Type Of Funding C3120 - Aust Philanthropy
Category 3120
UON Y
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Research Supervision

Number of supervisions

Completed0
Current4

Current Supervision

Commenced Level of Study Research Title Program Supervisor Type
2018 PhD Cellular Death and Survival of Pulmonary Cells in Mice and Cultured BEAS-2B Cells in Response to Titanium Dioxide Nanoparticles PhD (Environ & Occupat Hlth), Faculty of Health and Medicine, The University of Newcastle Principal Supervisor
2017 PhD Toxicology of Titanium Dioxide Nanoparticles PhD (Environ & Occupat Hlth), Faculty of Health and Medicine, The University of Newcastle Co-Supervisor
2017 PhD The Role of Olfactory Receptors in Inducing Allergic Inflammation PhD (Environ & Occupat Hlth), Faculty of Health and Medicine, The University of Newcastle Principal Supervisor
2015 Masters To determine whether the SmartCap Drive Fatigue Monitoring Device can help Detect Fatigue Related Medical Disorders in Mobile Plant Operators M Philosophy (Environ&OccHlth), Faculty of Health and Medicine, The University of Newcastle Co-Supervisor
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Dr He Wang

Position

Senior Lecturer
Chronic Respiratory Diseases
School of Health Sciences
Faculty of Health and Medicine

Contact Details

Email he.wang@newcastle.edu.au
Phone (02) 4921 7735
Fax (02) 4921 7053

Office

Room HA08
Building Hunter Building
Location Callaghan
University Drive
Callaghan, NSW 2308
Australia
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