Dr Harry Wang
Honorary Senior Lecturer
School of Health Sciences
- Email:he.wang@newcastle.edu.au
- Phone:(02) 4921 7735
Career Summary
Biography
I am a Senior Lecturer in Occupational in Health and Safety at the School of Health Sciences. I am also a Researcher interested in health effects of environmental and occupational factors.
RESEARCH and INNOVATION
My research focusses on environmental hazards and their health effects by investigating the toxicity of the environmental factors on organisms at molecular and cellular levels in cell, animal and human models. My research includes the following areas:
- Inflammatory and fibrotic effects of dust particles;
- Carcinogenic effects of airborne particles;
- Development of age-related diseases induced by environmental factors;
- Smoking and cancer risk;
- Environmental and occupational respiratory disease;
- Mutagenic effects of nanoparticles;
- Harmful effects of arsenic compounds;
- Respiratory health effects of chemical mixtures;
- Chemical induced autophagy, apoptosis and senescence and
- Chemical induced gene expressions reflected by RNA-seq and proteomics.
The long term goal of my research is to effectively prevent environmental and occupational disorders from occurring. The objectives of my current research, to pursue my long term goal, are to detect early and reversible changes induced by environmental and occupational factors and address them in a timely manner.
I used cell, rat and human models in my studies. The hazards under study are mainly airborne particles including nano-sized hazards. My research results contributed significantly to relevant areas. For example, my rat model research contributed to the guidelines for National and World Leadership to Prevent Workplace Illnesses and Injuries led by WHO, NIOSH, United Nations Environment Program (UNEP), and International Labor Organization (ILO). Five articles authored by me have been cited by those organizations to make recommendations for the prevention of silica-related illness. For another example, my paper “Cyto- and genotoxicity of ultrafine TiO2 particles in cultured human lymphoblastoid cells”, has received outstanding citations, indicating the impact of this paper on the scientific community. The results of my research generated a list of publications and more than 1,100 citations so far.
TEACHING and LEARNING
I teach in the postgraduate Work Health and Safety programs at UON as a course coordinator and senior lecturer and played the same roles in the undergraduate BEnvOSH program taught in Singapore. In my teaching, I encourage deep approach by providing hypothetical cases and case analyses. I enjoy teaching and believe it will help to systemizing and expanding my own knowledge.
SERVICE and ENGAGEMENT
At UON I was program coordinator for our BEnvOSH program taught in Singapore during January 2015 to September 2015. I am editorial board member for the following international journals:
Journal of Toxicology and Environmental Health
Austin Journal of Environmental Toxicology
Austin Pharmacology & Pharmaceutics
Journal of Respiratory Medicine and Lung Disease
I am also a full member of American Society of Toxicology
INTERNATIONAL COLLABORATIONS
I am currently collaborating with American researchers in Tulane University and Xavier University for molecular toxicology studies. The research focus is gene expression changes induced by oil spill chemicals. A number of publications have been generated in such collaborations.
Qualifications
- Doctor of Philosophy, University of Sydney
Keywords
- Autophagy and Apoptosis
- Black Lung
- Cellular Senescence
- Environmental and Occupational Respiratory Disease
- Gene Environmental Interactions
- Toxicity of Arsenic
- Toxicity of Chemical Mixtures
- Toxicity of Diesel Exhaust
- Toxicity of Silica and asbestos
- Toxicology of nanoparticles
Languages
- Mandarin (Mother)
- English (Fluent)
Professional Experience
Academic appointment
Dates | Title | Organisation / Department |
---|---|---|
26/10/2007 - 10/12/2014 |
Assistant Professor of Environmental and Occupational Health Sciences As a faculty member in the School of Public Health and Tropical Medicine, Dr. He Wang taught Occupational Health, Environmental Health Risk Assessment, Principles of Toxicology, Industrial Hygiene and Environmental Cancer Risk Inquiry for undergraduate, graduate and doctoral students. He also mentored master students, PhD candidates and postdoctoral fellows in toxicology research. |
Tulane University Global Environmental Health Sciences United States |
7/8/2003 - 18/10/2007 |
Senior Lecturer of Environmental and Occupational Health As a senior lecturer, Dr Wang taught public health and public health sciences to undergraduate students. He also taught Diseases of Occupation, Practical Occupational health, Occupational Toxicology for postgraduate students. He also played role as program coordinator for Master of Public Health and Master of Occupational Health and Safety programs. Dr Wang supervised MPH coursework students and Master of Occupational Health and Safety coursework students in research. Dr He Wang also supervised a PhD candidate in toxicological research as a main supervisor. |
Adelaide University Public health Australia |
Professional appointment
Dates | Title | Organisation / Department |
---|---|---|
10/4/1998 - 3/8/2007 |
Scientific Officer As a scientific officer, Dr Wang did research on respiratory health of coal miners, school children, newborn and premature infants, cystic fibrosis patients and patients with other respiratory diseases. Dr Wang was initially adjunct lecturer in the School and and promoted to adjunct senior lecturer in 2003. |
The University of New South Wales School of Women's and Children's Health Australia |
Teaching
Code | Course | Role | Duration |
---|---|---|---|
OHSE3640 |
Research Methods in EOHS The University of Newcastle, Singapore This is a research course focusing on the relationship between environmental/occupational factors and harmful health effects. |
Lecturer | 2/5/2016 - 29/7/2016 |
OHSE6010 |
Occupational Health The University of Newcastle - Faculty of Health and Medicine The course focus on the association between occupational hazards and occupational disorders. |
Lecturer and coordinator | 22/2/2016 - 3/6/2016 |
OHSE3640 |
Research Methods in EOHS The University of Newcastle, Singapore This is a course focusing on the association between occupational hazards and occupational disorders. |
Lecturer and Coordinator | 1/5/2017 - 28/7/2017 |
OHSE2610 |
Occupational Health The University of Newcastle, Singapore This is a course focusing on the association between occupational hazards and occupational disorders. |
Lecturer | 2/5/2016 - 29/7/2016 |
OHSE6010 |
Occupational Health The University of Newcastle - Faculty of Health and Medicine This course introduces students to the relationship between health and the work environment. It illustrates aspects of the structure and function of the human body and investigates the recognition of how hazards in the workplace can affect the body. |
Lecturer and Coordinator | 27/2/2017 - 23/6/2017 |
OHSE6090 |
Research inOccupational Health and Safety The University of Newcastle - Faculty of Health and Medicine This course introduces aspects of occupational health and safety. Epidemiology is the focus of the course. The course also covers some other research methods and critical appraisal of the literature. |
Lecturer and coordinator | 27/7/2015 - 6/11/2015 |
OHSE6090 |
Research inOccupational Health and Safety The University of Newcastle - Faculty of Health and Medicine This course focuses on epidemiological methods to study the association between occupational hazards and occupational disorders. |
Lecturer and Coordinator | 24/7/2017 - 3/11/2017 |
OHSE2610 |
Occupational Health The University of Newcastle, Singapore This is a course focusing on the association between occupational hazards and occupational disorders. |
Lecturer | 1/5/2017 - 28/7/2017 |
OHSE6010 |
Occupational Health The University of Newcastle - Faculty of Health and Medicine This course introduces students to the relationship between health and the work environment. It illustrates aspects of the structure and function of the human body and investigates the recognition of how hazards in the workplace can affect the body. |
Lecturer and Coordinator | 26/2/2018 - 8/6/2018 |
OHSE3640 |
Research Methods in EOHS The University of Newcastle, Singapore This is a research methods course. It covers research methods to investigate the association between environmental/occupational factors and the harmful effects. |
Lecturer | 4/5/2015 - 31/7/2015 |
OHSE6090 |
Research inOccupational Health and Safety The University of Newcastle - Faculty of Health and Medicine The course focus is epidemiological methods for studying the association between occupational hazards and occupational disorders. |
Lecturer and coordinator | 25/7/2016 - 7/11/2016 |
OHSE6010 |
Occupational Health The University of Newcastle - Faculty of Health and Medicine The course focus is association between occupational hazards and occupational disorders |
Lecturer and coordinator | 23/2/2015 - 5/6/2015 |
Publications
For publications that are currently unpublished or in-press, details are shown in italics.
Journal article (75 outputs)
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2020 |
Yu S, Wang F, Bi Y, Wang P, Zhang R, Bohatko-Naismith J, et al., 'Autophagy regulates the Wnt/GSK3ß/ß-catenin/cyclin D1 pathway in mesenchymal stem cells (MSCs) exposed to titanium dioxide nanoparticles (TiO
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2020 |
Wang P, Zhang R, Yu S, Lee C, Wang H, 'Simulative structure and binding sites of lyral with olfactory receptor 10J5 using computational prediction methods', Journal of Toxicology and Environmental Health - Part A: Current Issues, 83 1-8 (2020) [C1] Olfactory receptor (OR) genes are extensively distributed throughout the human organism. Although these receptors are predominantly located in the olfactory epithelium, binding be... [more] Olfactory receptor (OR) genes are extensively distributed throughout the human organism. Although these receptors are predominantly located in the olfactory epithelium, binding between odorant chemicals and corresponding ORs initiates downstream events in other tissues. In particular, exposure to allergen fragrances results in the induction of contact dermatitis. At present, current methodologies are limited in their ability to predict the consequences of fragrancy chemicals on humans. The aim of this study was designed to simulate the bindingstructure¿between lyral and OR10J5, a known allergen which produces contact dermatitis, and its corresponding OR OR10J5 in an effort to predict dermal outcomes using computational methods. Results demonstrated that binding between lyral and OR10J5 involved amino acid residues Phe104, Val105, Cyx178, Ile180, and Tyr258, respectively, which were located on binding sites of the receptor transmembrane 3(TM3), TM3, extracellular loop 2(EL2), EL2, TM6. Evidence indicates that computer simulating binding interactions occurred between an odorant chemical and its receptors which initiated downstream alterations accounting possibly for the observed in vivo contact dermatitis.
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2020 |
Zhang R, Wang P, Yu S, Hansbro P, Wang H, 'Computerized screening of G-protein coupled receptors to identify and characterize olfactory receptors', Journal of Toxicology and Environmental Health - Part A: Current Issues, 83 9-19 (2020) [C1] Olfactory receptors (ORs) are a group of G protein coupled receptors (GPCRs) that initiate chemical odorant signals. Although ORs are predominantly located in nasal epithelia to d... [more] Olfactory receptors (ORs) are a group of G protein coupled receptors (GPCRs) that initiate chemical odorant signals. Although ORs are predominantly located in nasal epithelia to detect smell, these receptors are also present in peripherally in non-nasal organs/tissues. Since the quality of life and cognitive and sensorial features of sense of smell are worsened in multiple chemical sensitivity due to the interaction of ORs with offending compounds, it is important to not only differentiate these receptors from other GPCRs but also characterize these organelles to understand the underlying mechanisms of smelling disorders. The aim of this study was develop computerized programs to differentiate ORs from GPCRs. The computer program was developed on the basis of widely accepted basic algorithms. It is noteworthy that an accuracy of 95.5% was attained, a level not achieved using other established techniques for screening of ORs from GPCRs. The high accuracy rate indicates that this method of differential identification appears reliable. Our findings indicate that this novel method may be considered as a tool for identification and characterization of receptors which might aid in therapeutic approaches to human chemical-mediated sensitization.
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2020 |
Zhang R, Wang P, Yu S, Wang H, 'Computational prediction methods to simulate structure and binding sites of coumarin with olfactory receptor 5P3', Journal of Toxicology and Environmental Health - Part A: Current Issues, 82 1199-1206 (2020) [C1]
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2019 |
Song W, Zhao J, Yan XS, Fang X, Huo DS, Wang H, et al., 'Mechanisms Associated with Protective Effects of Ginkgo Biloba Leaf Extracton in Rat Cerebral Ischemia Reperfusion Injury', Journal of Toxicology and Environmental Health - Part A: Current Issues, 82 1045-1051 (2019) [C1] Cerebral infarction occurs as a consequence of cerebral ischemia-reperfusion injury (CIRI). Ginkgo biloba leaf extract (GbE) is composed predominantly of active ingredients such a... [more] Cerebral infarction occurs as a consequence of cerebral ischemia-reperfusion injury (CIRI). Ginkgo biloba leaf extract (GbE) is composed predominantly of active ingredients such as flavonoids and terpene lactones and often used to treat cerebrovascular diseases. However, the mechanisms underlying the use of this herbal extract to treat cerebrovascular-mediated damage are not known. The aim of this study was to examine the effectiveness of administration GbE to ameliorate the observed consequences of CIRI. The following parameters were measured: (1) behavioral score (2) infarct area (3) the content of serum malondialdehyde (MDA) as well as activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) and (4) interleukin 6 (IL-6) and tumor necrosis factor-alpha (TNF-a) expression levels in the infarcted brain tissue. Data demonstrated that treatment with GbE to CIRI rats resulted in significant reduction in cerebral-infarcted area associated with improvement in behavioral score. GbE was found to decrease serum MDA levels concomitant with elevated activity levels of SOD and GSH-PX. Immunohistochemistry and Western blot analysis showed that GbE significantly lowered the levels of IL-6 and TNF-a in the infarcted brain tissue. Data suggest that GbE may be therapeutically effective in improving behavioral score in CIRI rats through reduction of oxidative stress and anti-inflammation in the cerebral infarction region.
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2019 |
Wu P, Yan XS, Zhou LL, Liu XL, Huo DS, Song W, et al., 'Involvement of apoptosis in the protective effects of Dracocephalum moldavaica in cerebral ischemia reperfusion rat model', Journal of Toxicology and Environmental Health - Part A: Current Issues, 82 1036-1044 (2019) [C1] An extract of Dracocephalum moldevica (DML) was found to exert protective effects on cerebral ischemia-reperfusion injury (CIRI); however, the mechanisms underlying the observed a... [more] An extract of Dracocephalum moldevica (DML) was found to exert protective effects on cerebral ischemia-reperfusion injury (CIRI); however, the mechanisms underlying the observed actions of this plant-derived mixture remain to be determined. Thus, the aim of this study was to examine the influence of DML on CIRI rat model induced by middle cerebral artery occlusion (MCAO). The following parameters were measured: (1) viable neurons in the infarcted area using Nissl staining; and (2) immunohistochemistry and Western blot were employed to determine protein expression levels of p53, bcl-2 associated X protein (bax) and B-cell lymphoma-2 (bcl-2), three biomarkers of apoptosis. MCAO significantly decreased the number of viable cortical pyramidal neurons in the infarcted area, while treatment with DML extract significantly elevated the number of viable neurons. MCAO was found to significantly elevate in gene expression levels of p53 and protein expression levels bax accompanied by diminished protein expression levels of bcl-2. Prior administration of DML extract produced marked reduction in gene expression levels of p53 and protein expression levels bax but increased in protein expression levels of bcl-2. Data suggested apoptosis was initiated in MCAO and that DML was effective in treating CIRI via an anti-apoptotic action as evidenced by inhibition of gene expression levels of p53 and protein expression levels of bax with concomitant elevation in protein expression levels of bcl-2. Our findings suggest that extract of DML may prove beneficial in treatment of cerebrovascular disorders.
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2019 |
Zhang M, Zhang YQ, Wei XZ, Lee C, Huo DS, Wang H, Zhao ZY, 'Differentially expressed long-chain noncoding RNAs in human neuroblastoma cell line (SH-SY5Y): Alzheimer s disease cell model', Journal of Toxicology and Environmental Health - Part A: Current Issues, 82 1052-1060 (2019) [C1] A number of complex human diseases including neurological diseases is characterized by dysregulation of long-chain noncoding RNA (lncRNA). The pathogenesis of Alzheimer's dis... [more] A number of complex human diseases including neurological diseases is characterized by dysregulation of long-chain noncoding RNA (lncRNA). The pathogenesis of Alzheimer's disease (AD), a neurodegenerative disorder is believed to involve alterations in lncRNAs. However, the specific lncRNAs modified in AD remain to be determined. The aim of this study was to identify lncRNAs associated with AD using human neuroblastoma cell line (SH-SY5Y) treated with beta-amyloid (Aß) as a model of this disease. The differential expressions of lncRNA were compared between beta-amyloid (Aß) SH-SY5Y cells and normal SH-SY5Y cells utilizing Illumina X10 gene sequencing. The differential expression profiles of amyloid (Aß)-treated SH-SY5Y cells were determined and verified by qRT-PCR method. The expression levels of lncRNA were expressed by calculating the abundance of FPKM (measure gene expression). The differential expression of log2 (multiple change) >1 or log2 (multiple change) < -1 had statistical significance (P<.05). The differential expression profiles of amyloid (Aß)-treated SH-SY5Y cells showed 40 lncRNA were up-regulated, while 60 lncRNA were down-regulated. GO and KEGG analysis demonstrated that differentially expressed genes were predominantly involved in the mitogen-activated protein kinase (MAPK) signaling pathway, p53 signaling pathway, hepatitis B, cell cycle, post-translational protein modification, and regulation. In conclusion, approximately 100 dysregulated lncRNA transcripts were found in amyloid (Aß)-treated SH-SY5Y cells and these lncRNAs may play an important role in the occurrence and development of AD through altered signal pathways.
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2019 |
Wang L, Pei JH, Jia JX, Wang J, Song W, Fang X, et al., 'Inhibition of oxidative stress by testosterone improves synaptic plasticity in senescence accelerated mice', Journal of Toxicology and Environmental Health - Part A: Current Issues, 82 1061-1068 (2019) [C1] It is well known that synaptic plasticity is associated with cognitive performance in Alzheimer¿s disease (AD). Testosterone (T) is known to exert protective effects on cognitive ... [more] It is well known that synaptic plasticity is associated with cognitive performance in Alzheimer¿s disease (AD). Testosterone (T) is known to exert protective effects on cognitive deficits in AD, but the underlying mechanisms of androgenic action on synaptic plasticity remain unclear. Thus, the aim of this study was to examine the protective mechanism attributed to T on synaptic plasticity in an AD senescence accelerated mouse prone 8 (SAMP8) model. The following parameters were measured: (1) number of intact pyramidal cells in hippocampal CA1 region (2) phosphorylated N-methyl-D-aspartate receptor-1 (p-NMDAR1) and (3) phosphorylated calmodulin-dependent protein kinase II (p-CaMKII). In addition, the content of whole brain malondialdehyde (MDA) as well as activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) were determined. Treatment with T significantly elevated the number of intact pyramidal cells in hippocampal CA1 region and markedly increased hippocampal protein and mRNA expression levels of p-NMDAR1 and p-CaMK II. Further, T significantly decreased whole brain MDA levels accompanied by elevated activities of SOD and GSH-Px. Data suggest that the protective effects of T on synaptic plasticity in a mouse AD model may be associated with reduction of oxidant stress.
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2019 |
Zhao ZY, Zhang YQ, Zhang YH, Wei XZ, Wang H, Zhang M, et al., 'The protective underlying mechanisms of Schisandrin on SH-SY5Y cell model of Alzheimer s disease', Journal of Toxicology and Environmental Health - Part A: Current Issues, 82 1019-1026 (2019) [C1] The extract of Schisandrin a traditional Chinese medicine was postulated to be effective in prevention and treatment of Alzheimer¿s disease (AD). The aim of this study was to exam... [more] The extract of Schisandrin a traditional Chinese medicine was postulated to be effective in prevention and treatment of Alzheimer¿s disease (AD). The aim of this study was to examine the underlying protective actions of Schizandrin using a human neuroblastoma cell line (SH-SY5Y). In particular Schizandrin-mediated effects on expression of glycogen synthase kinase (GSK)-3ß, protein kinase B (Akt) and Tau protein, known to be altered in AD were determined. In preliminary assays, various concentrations of Schisandrin were incubated SH-SY5Y cells to establish effects on cell viability and potential toxicity in further experimentation. Amyloid-ß (Aß1-42) peptide 10 µmol/L was used to induce in vitro AD model in SH-SY5Y. Exposure to Aß1-42 significantly reduced cell viability. Treatment with Schisandrin to Aß1-42 exposed cells increased cell viability compared to amyloid peptide; however only the 10 µmol/L Schisandrin concentration was effective in restoring cell viability to control. Western blot analysis demonstrated that Aß1-42 produced a significant decrease in p-Akt protein expression levels accompanied by marked elevation in p-tau and p-GSK-3ß protein expression levels. Addition of 10 µmol/L Schisandrin to amyloid-treated SH-SY5Y cells was found to significantly increase protein expression levels of p-Akt associated with reduction in expression levels of p-tau and p-GSK-3ß protein. Treatment with 10 µmol/L Schisandrin of SH-SY5Y cells with the p-Akt inhibitor LY294002 demonstrated that the herbal-induced rise in p-Akt protein expression was diminished by this inhibitor indicating that signal transduction occurred in the observed cellular effects. Evidence indicates that Schisandrin inhibition of Aß1-42 -mediated cellular damage in AD neurons may involve activation of the PI3K/Akt signaling pathway where up-regulation of p-Akt activity consequently leads downstream to decreased activity of p-GSK-3ß phosphorylation accompanied by reduced tau protein. Consequently, restoration of neuronal cell viability was noted. Our findings suggest that the use of Schisandrin may be considered beneficial as a therapeutic agent in AD.
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2019 |
Huo DS, Sun JF, Cai ZP, Yan XS, Wang H, Jia JX, Yang ZJ, 'The protective mechanisms underlying Ginsenoside Rg1 effects on rat sciatic nerve injury', Journal of Toxicology and Environmental Health - Part A: Current Issues, 82 1027-1035 (2019) [C1] Ginsenoside Rg1 (GsRg1), derived from the herb Ginseng, was found to exert protective effects in nerve injury; however, the mechanisms underlying these effects remain to be determ... [more] Ginsenoside Rg1 (GsRg1), derived from the herb Ginseng, was found to exert protective effects in nerve injury; however, the mechanisms underlying these effects remain to be determined. Oxidant stress and apoptosis are known to be involved in sciatic nerve injury. Thus, the aim of this study was to examine whether GsRg1 was able to modify sciatic nerve injury in a rat model. The following parameters were measured: (1) number of spinal cord motoneurons by Nissl staining, (2) oxidation parameters including spinal cord malondialdehyde (MDA) levels and activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) as well as (3) involvement of apoptosis by determining caspase-3 and X-linked inhibitor of apoptosis protein (XIAP) by immunohistochemistry and Western blot. The number of spinal cord motoneurons was significantly reduced after sciatic nerve injury, while treatment with GsRg1 markedly elevated cell number. Sciatic nerve injury markedly increased spinal cord MDA content concomitant with reduced activities of SOD and GSH-Px. GsRg1 significantly decreased MDA content accompanied by elevated activities of SOD and GSH-Px. Further nerve injury significantly diminished protein expression levels of XIAP accompanied by elevated protein expression levels of caspase-3 in the spinal cord. GsRg1 markedly increased protein expression levels of XIAP, but significantly reduced protein expression levels of caspase-3. Data suggest that the protective effects of GsRg1 in sciatic nerve injury may be associated with reduced oxidative stress involving anti-apoptotic pathways.
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2019 |
Yan XS, Yang ZJ, Jia JX, Song W, Fang X, Cai ZP, et al., 'Protective mechanism of testosterone on cognitive impairment in a rat model of Alzheimer's disease', Neural Regeneration Research, 14 649-657 (2019) [C1] Cognitive dysfunction in Alzheimer's disease is strongly associated with a reduction in synaptic plasticity, which may be induced by oxidative stress. Testosterone is benefic... [more] Cognitive dysfunction in Alzheimer's disease is strongly associated with a reduction in synaptic plasticity, which may be induced by oxidative stress. Testosterone is beneficial in learning and memory, although the underlying protective mechanism of testosterone on cognitive performance remains unclear. This study explored the protective mechanism of a subcutaneous injection of 0.75 mg testosterone on cognitive dysfunction induced by bilateral injections of amyloid beta 1-42 oligomers into the lateral ventricles of male rats. Morris water maze test results demonstrated that testosterone treatment remarkably reduced escape latency and path length in Alzheimer's disease rat models. During probe trials, testosterone administration significantly elevated the percentage of time spent in the target quadrant and the number of platform crossings. However, flutamide, an androgen receptor antagonist, inhibited the protective effect of testosterone on cognitive performance in Alzheimer's disease rat models. Nissl staining, immunohistochemistry, western blot assay, and enzyme-linked immunosorbent assay results showed that the number of intact hippocampal pyramidal cells, the dendritic spine density in the hippocampal CA1 region, the immune response and expression level of postsynaptic density protein 95 in the hippocampus, and the activities of superoxide dismutase and glutathione peroxidase were increased with testosterone treatment. In contrast, testosterone treatment reduced malondialdehyde levels. Flutamide inhibited the effects of testosterone on all of these indicators. Our data showed that the protective effect of testosterone on cognitive dysfunction in Alzheimer's disease is mediated via androgen receptors to scavenge free radicals, thereby enhancing synaptic plasticity.
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2019 |
Yu S, Mu Y, Zhang X, Li J, Lee C, Wang H, 'Molecular mechanisms underlying titanium dioxide nanoparticles (TiO2NP) induced autophagy in mesenchymal stem cells (MSC)', Journal of Toxicology and Environmental Health - Part A: Current Issues, 82 997-1008 (2019) [C1]
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2018 |
Jia JX, Yan XS, Song W, Fang X, Cai ZP, Huo DS, et al., 'The protective mechanism underlying phenylethanoid glycosides (PHG) actions on synaptic plasticity in rat Alzheimer s disease model induced by beta amyloid 1-42', Journal of Toxicology and Environmental Health - Part A: Current Issues, 81 1098-1107 (2018) [C1] Phenylethanoid glycosides (PHG), derived from Herba cistanche, were found to exert protective effects on cognitive dysfunctions by improving synaptic plasticity in Alzheimer¿s dis... [more] Phenylethanoid glycosides (PHG), derived from Herba cistanche, were found to exert protective effects on cognitive dysfunctions by improving synaptic plasticity in Alzheimer¿s disease (AD) rat model. However, the mechanisms underlying these effects of PHG on synaptic plasticity remain to be determined. Thus the aim of this study was to examine the influence of PHG on synaptic plasticity in male AD rat model induced by bilateral central nervous system ventricle injections of beta amyloid 1¿42 oligomers (Aß1¿42). The following parameters were measured: (1) number of intact pyramidal cells in hippocampal CA1 region by Nissl staining, (2) post synaptic density 95 (PSD-95), phosphorylated N-methyl-D-aspartate receptor-1(p-NMDAR1) and (3) phosphorylated Tau protein (p-Tau) by immunohistochemistry and western blot. In addition, the content of malondialdehyde (MDA) and activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) were determined. Aß1-42 lowered the number of intact pyramidal cells in hippocampal CA1 region. In contrast, treatment with PHG significantly elevated this cell number. Aß1-42 significantly diminished protein expression levels of PSD-95 accompanied by elevated protein expression levels of p-NMDAR1 and p-Tau. PHG markedly increased protein expression levels of PSD-95, but significantly reduced protein expression levels of p-NMDAR1 and p-Tau. Further, Aß1-42 markedly increased MDA content concomitantly with reduced activities of SOD and GSH-Px. PHG significantly decreased MDA content accompanied by elevated activities of SOD and GSH-Px. Data suggest that the protective effects of PHG on synaptic plasticity may involve inhibition of cytotoxicity-mediated by Aß-1-42 administration and reduction of oxidant stress.
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2018 |
Wu P, Yan XS, Zhang Y, Huo DS, Song W, Fang X, et al., 'The protective mechanism underlying total flavones of Dracocephalum (TFD) effects on rat cerebral ischemia reperfusion injury', Journal of Toxicology and Environmental Health - Part A: Current Issues, 81 1199-1206 (2018) [C1] Previously, total flavones of Dracocephalum (TFD), derived from Dracocephalum, were found to exert protective effects in cerebral ischemia reperfusion injury (CIRI) in middle cere... [more] Previously, total flavones of Dracocephalum (TFD), derived from Dracocephalum, were found to exert protective effects in cerebral ischemia reperfusion injury (CIRI) in middle cerebral artery occlusion (MCAO) rat model. However, the mechanisms underlying these observed effects of TFD on MCAO-induced rats still remain to be determined. Therefore, the aim of this study was to examine whether TFD alleviated MCAO through mechanisms involving anti-inflammatory and anti-apoptotic using MCAO rats. The following parameters were measured: (1) percentage (%) area of brain infarction; (2) serum levels of inflammatory cytokines, including tumor necrosis factor alpha (TNF-a) and interleukin-6 (IL-6) and (3) expression protein levels of caspase-3 and AMP-activated protein kinase (AMPK). Results showed that MCAO significantly increased the % area of brain infarction, while TFD administration in these animals markedly reduced % area of brain infarction. A significant elevation on serum levels of TNF-a and IL-6 was noted with MCAO which was markedly reduced by TFD. In addition, MCAO produced a significant rise in protein expression levels of caspase-3 and AMPK. In contrast, TFD markedly lowered protein expression levels of caspase-3 and AMPK. Data suggest that the protective effects of TFD in MCAO model animals may involve inhibition of inflammatory mediator release associated with apoptosis through down regulation of AMPK signaling pathway.
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2018 |
Zhang M, Zheng H, Zhang X, Tian X, Xu S, Liu Y, et al., 'Involvement of nerve growth factor in mouse hippocampal neuronal cell line (HT22) differentiation and underlying role of DNA methyltransferases', Journal of Toxicology and Environmental Health - Part A: Current Issues, 81 1116-1122 (2018) [C1] DNA methylation is an epigenetic event involved in regulation of gene transcription during cell differentiation. DNA methyltransferases (DNMT) play a role in differentiation of ne... [more] DNA methylation is an epigenetic event involved in regulation of gene transcription during cell differentiation. DNA methyltransferases (DNMT) play a role in differentiation of neural stem cells into neurons. The aim of this study was to determine whether nerve growth factor (NGF) was involved in differentiation of mouse hippocampal neuronal cell line (HT22) as assessed by IncuCyte. Quantitative PCR and western blot were used to measure gene and protein expression of DNMT as well as the activity of DNMTs. Treatment with NGF was found to upregulate both gene and protein expressions as well as total activity of DNMTs in differentiating HT22 cells. Compared to undifferentiating cells, the percentage of differentiating cells at S phase increased significantly when incubated with NGF. In undifferentiated cells, NGF failed to induce gene and protein expressions and activity of DNMTs. Data demonstrate that differentiation of HT22 cells by exposure to NGF involve the activation of DNMTs pathway.
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2017 |
Zhao ZY, Gao YY, Gao L, Zhang M, Wang H, Zhang CH, 'Protective effects of bellidifolin in hypoxia-induced in pheochromocytoma cells (PC12) and underlying mechanisms', Journal of Toxicology and Environmental Health - Part A: Current Issues, 80 1187-1192 (2017) [C1] Bellidifolin, a xanthone compound derived from plants of Gentiana species, is known to exert a variety of pharmacological activities including anti-oxidation, anti-inflammatory an... [more] Bellidifolin, a xanthone compound derived from plants of Gentiana species, is known to exert a variety of pharmacological activities including anti-oxidation, anti-inflammatory and antitumor actions as well as a protective effect on cerebral ischemic nerve injury. The aim of this study was to examine the protective effects of bellidifolin on nerve injury produced by hypoxia and possible underlying mechanisms using pheochromocytoma cells (PC12). Data showed that the viability of PC12 cells subjected to hypoxia resulted in a significant decrease; however; pretreatment with certain concentrations of bellidifolin (20 or 40¿µmol/L) prior to hypoxia significantly increased the survival rate. The results of immunohistochemical staining analysis revealed that there were no marked alterations in the expression of pERK protein between all bellidifolin groups while the expression of p-p38MAPK protein was significantly enhanced by hypoxia. Pretreatment with different concentrations of bellidifolin followed by hypoxia significantly decreased the expression of p-p38MAPK protein. The results of western blot analysis showed that hypoxia induced the expression of the MAPK signaling pathway downstream of the key apoptosis factor caspase-3. Compared to hypoxia, the expression of caspase-3 in the presence of belliidifolin was significantly lower. Data suggest that bellidifolin may contribute to the protective effects associated with nerve injury initiated by hypoxia by mechanisms related to inhibition of cell apoptosis independent of the ERK pathway, but may involve blockade of p38MAPK signaling pathway activation and downstream caspase-3 expression.
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2017 |
Jia JX, Yan XS, Cai ZP, Song W, Huo DS, Zhang BF, et al., 'The effects of phenylethanoid glycosides, derived from Herba cistanche, on cognitive deficits and antioxidant activities in male SAMP8 mice', Journal of Toxicology and Environmental Health - Part A: Current Issues, 80 1180-1186 (2017) [C1] Cognitive deficits are closely associated with hippocampal synaptic changes. Phenylethanoid glycosides (PhG), derived from Herba cistanche, are known to exert protective effects o... [more] Cognitive deficits are closely associated with hippocampal synaptic changes. Phenylethanoid glycosides (PhG), derived from Herba cistanche, are known to exert protective effects on cognitive deficits in Alzheimer¿s disease (AD); however, the underlying mechanisms of this herbal extract on cognitive performance remain unclear. The aim of this study was thus to examine the protective mechanism attributed to PhG on cognitive deficits in an AD senescence accelerated mouse prone 8 (SAMP8) model. Cognitive deficit parameters examined included (1) Morris water maze (MWM) assessing cognitive performance and (2) quantification of dendritic spine density in hippocampal CA1 region by Golgi staining, a molecular biomarker of synaptic function. In addition, levels of malondialdehyde (MDA) and activities of superoxide dismutase (SOD) and gluthathione peroxidase (GSH-Px) were determined to examine the potential role of oxidant processes in cognitive dysfunction. Data showed that PhG significantly decreased escape latency and path length, associated with a rise in the percentage of time spent in the target quadrant and number of platform crossings. In addition, PhG significantly increased dendritic spine density in the hippocampal CA1 region accompanied by elevated expression levels of synaptophysin (SYN) and post synaptic density 95 (PSD-95), reduced MDA content, and elevated the activities of SOD and GSH-Px. Data suggest that the ability of PhG to ameliorate cognitive deficits in SAMP8 mice may be related to promotion in synaptic plasticity involving antioxidant processes.
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2017 |
Zhang M, Zheng HX, Gao YY, Zheng B, Liu JP, Wang H, et al., 'The influence of Schisandrin B on a model of Alzheimer s disease using ß-amyloid protein Aß Schisandrin B, an active substance, is derived from Chinese herb fruit Wuweizi, which exerts various pharmacological activities and has displayed significant beneficial effects in... [more] Schisandrin B, an active substance, is derived from Chinese herb fruit Wuweizi, which exerts various pharmacological activities and has displayed significant beneficial effects in ameliorating Alzheimer¿s disease (AD). The aim of this study was to further extend our examination for the use of schisandrin B extract in the potential treatment of AD effects by investigating DNA methylation (DNMT), known to be modified in this disease using SH-SY5Y neuronal cell line exposed to ß-amyloid protein (Aß1-42). In particular, the purpose of this investigation was to examine alterations in mRNA and protein expression of DNMT. Data demonstrated that schisandrin B blocked Aß1-42-mediated injury in SH-SY5Y neuronal cell line as evidenced by a restoration of cellular morphology and cell viability to approximate control levels at the highest 10¿µg/ml Schisandrin B. Incubation with Aß1-42 significantly decreased mRNA and protein expression of DNMT3A and DNMT1 in SH-SY5Y neuronal cell line. Incubation with Aß1-42 followed by 24 treatment with schisandrin B significantly inhibited the Aß1-42 -induced changes in mRNA and protein expression of DNMT3A and DNMT3B in a concentration-dependent manner. It is of interest that the mRNA expression of DNMT3A and DNMT1 were significantly higher than control. Data thus indicate schisandrin B was effective in inhibiting the actions of Aß1-42 on cell survival and morphology and that DNA methylation may be associated with the beneficial findings.
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2017 |
Jia JX, Zhang Y, Wang ZL, Yan XS, Jin M, Huo DS, et al., 'The inhibitory effects of Dracocephalum moldavica L. (DML) on rat cerebral ischemia reperfusion injury', Journal of Toxicology and Environmental Health - Part A: Current Issues, 80 1206-1211 (2017) [C1] Ischemia reperfusion injury (IRI) is closely associated with oxidative stress and inflammatory responses. Dracocephalum moldavica L. (DML), a Chinese herbal medicine is known to e... [more] Ischemia reperfusion injury (IRI) is closely associated with oxidative stress and inflammatory responses. Dracocephalum moldavica L. (DML), a Chinese herbal medicine is known to exert protective effects on myocardial ischemia reperfusion injury in rats by inhibiting oxidation damage and inflammatory reactions. However, the effectiveness of DML in cerebral ischemia reperfusion injury (CIRI) as a protective substance and the underlying mechanisms remain to be determined. The aim of this study was thus to examine the influence of DML on CIRI using a rat model induced by 2-h transient middle cerebral artery occlusion (MCAO) produced by intraluminal suture blockade followed by 22¿h reperfusion. The parameters determined include neurological behavior, histochemical assessment of cerebral infarct volume, and determination of various metabolic biomarkers. Data showed that DML markedly improved neurobehavioral scores and reduced cerebral edema and infarction. In addition, DML significantly reduced malondialdehyde (MDA) content and elevated activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px), in addition, marked decrease in levels of interleukin-6 (IL-6), interleukin-8 (IL-8), and tumor necrosis factor-a (TNF-a). Data suggest that the protective effects of DML on CIRI may be related to processes involving antioxidation and anti-inflammation.
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2017 |
Mu Y, Wang R, Wang H, 'Programmed Cell Death of Cultured A549 Lung Ep-ithelial Cells Induced by Sodium Ar-senite Exposure.', Journal of Medicinal Chemistry and Toxicology, 2 85-89 (2017) [C1]
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2017 |
Yu S, Bohatko-Naismith J, Zhang X, Zhou X, Wang P, Wang H, 'Cellular responses in titanium dioxide nanoparticle cytotoxicity studies: parts of the map waiting to be composed', Journal of Medicinal Chemistry and Toxicology, 2 1-9 (2017) [C1]
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2017 |
Zheng Q-N, Wang J, Zhou H-B, Niu S-F, Liu Q-L, Yang Z-J, et al., 'Effectiveness of Amygdalus mongolica oil in hyperlipidemic rats and underlying antioxidant processes.', Journal of toxicology and environmental health. Part A, 80 1193-1198 (2017) [C1]
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2017 |
Liu YZ, Zhang L, Roy-Engel AM, Saito S, Lasky JA, Wang G, Wang H, 'Carcinogenic effects of oil dispersants: A KEGG pathway-based RNA-seq study of human airway epithelial cells', Gene, 602 16-23 (2017) [C1] The health impacts of the BP oil spill are yet to be further revealed as the toxicological effects of oil products and dispersants on human respiratory system may be latent and co... [more] The health impacts of the BP oil spill are yet to be further revealed as the toxicological effects of oil products and dispersants on human respiratory system may be latent and complex, and hence difficult to study and follow up. Here we performed RNA-seq analyses of a system of human airway epithelial cells treated with the BP crude oil and/or dispersants Corexit 9500 and Corexit 9527 that were used to help break up the oil spill. Based on the RNA-seq data, we then systemically analyzed the transcriptomic perturbations of the cells at the KEGG pathway level using two pathway-based analysis tools, GAGE (generally applicable gene set enrichment) and GSNCA (Gene Sets Net Correlations Analysis). Our results suggested a pattern of change towards carcinogenesis for the treated cells marked by upregulation of ribosomal biosynthesis (hsa03008) (p¿=¿1.97E¿-¿13), protein processing (hsa04141) (p¿=¿4.09E¿-¿7), Wnt signaling (hsa04310) (p¿=¿6.76E¿-¿3), neurotrophin signaling (hsa04722) (p¿=¿7.73E¿-¿3) and insulin signaling (hsa04910) (p¿=¿1.16E¿-¿2) pathways under the dispersant Corexit 9527 treatment, as identified by GAGE analysis. Furthermore, through GSNCA analysis, we identified gene co-expression changes for several KEGG cancer pathways, including small cell lung cancer pathway (hsa05222, p¿=¿9.99E¿-¿5), under various treatments of oil/dispersant, especially the mixture of oil and Corexit 9527. Overall, our results suggested carcinogenic effects of dispersants (in particular Corexit 9527) and their mixtures with the BP crude oil, and provided further support for more stringent safety precautions and regulations for operations involving long-term respiratory exposure to oil and dispersants.
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2016 |
Liu Y, Li Y, Yang T, Yang J, Wang H, Wu G, 'Acute changes in murine hippocampus and olfactory bulb after nasal instillation of varying size cerium dioxide particles', JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A-CURRENT ISSUES, 79 869-877 (2016) [C1]
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2016 |
Huo DS, Sun JF, Zhang B, Yan XS, Wang H, Jia JX, Yang ZJ, 'Protective effects of testosterone on cognitive dysfunction in Alzheimer s disease model rats induced by oligomeric beta amyloid peptide 1-42', Journal of Toxicology and Environmental Health - Part A: Current Issues, 79 856-863 (2016) [C1] Cognitive dysfunction is known to be influenced by circulating sex steroidal hormones. The aim of this study was to examine the protective effect and possible protective mechanism... [more] Cognitive dysfunction is known to be influenced by circulating sex steroidal hormones. The aim of this study was to examine the protective effect and possible protective mechanism of testosterone (T) on cognitive performance in male rats induced by intrahippocampal injections of beta amyloid 1-42 oligomers (Aß1-42). Treatment with T as evidenced by the Morris water maze (MWM) test significantly shortened escape latency and reduced path length to reach the platform compared to the control (C). During probe trials, the T group displayed a significantly greater percent of time in the target quadrant and improved the number of platform crossings compared with C, flutamide (F), an antiandrogen, and a combined F and T group. Flutamide markedly inhibited the influence of T on cognitive performance. Following Nissl staining, the number of intact pyramidal cells was significantly elevated in the T group, and the effect of T was blocked by F. Immunohistochemisty and Western blot analysis showed that the protein expression level of Aß 1-42 was markedly decreased and expression levels of synaptophysin (SYN) significantly increased with T, while F inhibited all T-mediated effects. Our data suggest that the influence of T on cognitive performance was mediated via androgen receptors (AR) to remove beta amyloid, which leads to enhanced synaptic plasticity.
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2016 |
Jia JX, Cui CL, Yan XS, Zhang BF, Song W, Huo DS, et al., 'Effects of testosterone on synaptic plasticity mediated by androgen receptors in male SAMP8 mice', Journal of Toxicology and Environmental Health - Part A: Current Issues, 79 849-855 (2016) [C1] Synaptic changes are closely associated with cognitive deficits. In addition, testosterone (T) is known to exert regulative effects on synaptic plasticity. T may improve cognitive... [more] Synaptic changes are closely associated with cognitive deficits. In addition, testosterone (T) is known to exert regulative effects on synaptic plasticity. T may improve cognitive deficits in Alzheimer¿s disease (AD) patients, but the underlying mechanisms of androgenic action on cognitive performance remain unclear. The aim of this study was thus to examine the protective mechanism attributed to T on cognitive performance in an AD senescence, accelerated mouse prone 8 (SAMP8) animal model. Using Golgi staining to quantify the dendritic spine density in hippocampal CA1 region, molecular biomarkers of synapse function were analyzed using immunohistochemistry and western blot. T significantly increased the dendritic spine density in hippocampal CA1 region, while flutamide (F) inhibited these T-mediated effects. Immunohistochemistry and western blot analysis showed that the expression levels of brain derived neurotrophic factor (BDNF), postsynaptic density 95 (PSD-95), and p-cyclic-AMP response element binding protein (CREB)/CREB levels were significantly elevated in the T group, but F reduced the T-induced effects in these biomarkers to control levels. There were no significant differences in the expression levels of PSD-95, BDNF, and p-CREB/CREB between C and F. These findings indicate that the effects of T on improvement in synaptic plasticity were mediated via androgen receptor (AR). It is conceivable that new treatments targeted toward preventing synaptic pathology in AD may involve the use of androgen-acting drugs.
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2016 |
Major D, Derbes RS, Wang H, Roy-Engel AM, 'Effects of corexit oil dispersants and the WAF of dispersed oil on DNA damage and repair in cultured human bronchial airway cells, BEAS-2B', Gene Reports, 3 22-30 (2016) [C1] Large quantities of dispersants were used as a method to disperse the roughly 210 million gallons of spilled crude oil that consumed the Gulf of Mexico. Little is known if the oil... [more] Large quantities of dispersants were used as a method to disperse the roughly 210 million gallons of spilled crude oil that consumed the Gulf of Mexico. Little is known if the oil-dispersant and oil-dispersant mixtures on human airway BEAS-2B epithelial cells. Here we present the cytotoxic and genotoxic in vitro effects on the human lung cells BEAS-2B following exposure to and oil-dispersant mixtures on human airway BEAS-2B epithelial cells. Here we present the cytotoxic and genotoxic in vitro effects on the human lung cells BEAS-2B following exposure to Corexit dispersants EC9500 and EC9527, Water Accommodated Fraction (WAF) -crude, WAF-9500 + Oil, and WAF-9527 + Oil. Cellular cytotoxicity to WAF-dispersed oil samples was observed at concentrations greater than 1000 ppm with over 70% of observed cellular death. At low concentration exposures (100 and 300 ppm) DNA damage was evidenced by the detection of single strand breaks (SSBs) and double strand breaks (DSBs) as measured by alkaline and neutral comet assay analyses. Immunoblot analyses of the phosphorylated histone H2A.X (¿-H2A.X) and tumor suppressor p53 protein confirmed activation of the DNA damage response due to the exposure-induced DNA breaks. Although, many xenobiotics interfere with DNA repair pathways, in vitro evaluation of the nucleotide excision repair (NER) and DSB repair pathways appear to be unaffected by the oil-dispersant mixtures tested. Overall, this study supports that oil-dispersant mixtures induce genotoxic effects in culture.
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2016 |
Liu Y-Z, Roy-Engel AM, Baddoo MC, Flemington EK, Wang G, Wang H, 'The impact of oil spill to lung health--Insights from an RNA-seq study of human airway epithelial cells.', Gene, 578 38-51 (2016) [C1]
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2015 |
Sun Y, Shao H, Wang H, 'Occupational diseases prevention and control in China: a comparison with the United States', Journal of Public Health (Germany), 23 379-386 (2015) Aim: Occupational diseases have become an important health issue and social problem for countries throughout the world. The United States and China face the serious situation of o... [more] Aim: Occupational diseases have become an important health issue and social problem for countries throughout the world. The United States and China face the serious situation of occupational diseases prevention and control. Methods: This paper compared the prevention and control of occupational diseases in the United States and China in the following aspects: status, law and related agencies, definition and categories, surveillance and reporting systems, diagnosis and identification. Results: China faces more challenges in occupational diseases prevention and control. Conclusion: There is a gap in the overall strength of occupational disease prevention and control between the US and China. There is a lot of work to do for China.
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2015 |
Zhang M, Huo DS, Cai ZP, Shao G, Wang H, Zhao ZY, Yang ZJ, 'The Effect of Schizandrol A-Induced DNA Methylation on SH-SY5YAB 1-40 Altered Neuronal Cell Line: A Potential Use in Alzheimers Disease', Journal of Toxicology and Environmental Health - Part A: Current Issues, 78 1321-1327 (2015) [C1] Disturbances in DNA methylation are postulated to result in various central nervous system diseases including Alzheimers disease (AD). The SH-SY5Y neuronal cell line treated with ... [more] Disturbances in DNA methylation are postulated to result in various central nervous system diseases including Alzheimers disease (AD). The SH-SY5Y neuronal cell line treated with Aß1-40 (5 mol/L) protein is considered to be a model of AD. Hence the aim of this study was to examine the influence of Schizandrol A, a plant extract, on DNA methylation in SH-SY5Y cells exposed to Aß1-40. Aß1-40 were incubated with varying concentrations of Sehizandrol A to a final concentration of 1 (low), 3 (intermediate) or 9 g/ml (high). Exposure of SH-SY5Y with Aß1-40 reduced viability, and altered cellular morphology and mRNA expression of DNA methyltransferase (DNMT3A) and DNMT3B. Treatment with 1 or 3 g/ml Sehizandrol A resulted in normal cell morphology as well as elevated cell number, enhanced viability, and increased mRNA expression of DNMT3A and DNMT3B compared to saline. However, an increase in Sehizandrol A to 9 g/ml produced a fall in cell viability, as well as a decrease in mRNA DNMT3A and DNMT3B expression to control levels. Data demonstrated that Schizandrol A at 1 or 3 g/ml improved cell morphological appearance and viability of Aß1-40 injured SH-SY5Y cells by an enhanced DNA methylation pathway.
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2015 |
Jia JX, Cui CL, Song W, Yan XS, Huo DS, Wang H, Yang ZJ, 'Effects of Testosterone Treatment on Synaptic Plasticity and Behavior in Senescence Accelerated Mice', Journal of Toxicology and Environmental Health - Part A: Current Issues, 78 1311-1320 (2015) [C1] Learning and memory are known to be influenced by circulating sex steroidal hormones and these behavioral processes are diminished in aging. Thus, the aim of this study was to exa... [more] Learning and memory are known to be influenced by circulating sex steroidal hormones and these behavioral processes are diminished in aging. Thus, the aim of this study was to examine the mechanism underlying testosterone-induced effects on cognitive performance in the senescence accelerated mouse P8 (SAMP8) model. Treatment with testosterone (T) as evidenced by the Morris water maze test produced a significantly shorter escape latency and reduced path length to reach the platform compared to the control (C). No significant differences were noted in mean swim speed among all groups. During the probe trials, the T group spent a significantly greater percent of time in the target quadrant and improved the number of platform crossings. Flutamide (F), an antiandrogen, significantly inhibited the effects of T on behavioral and memory performances indicators. Following Nissl staining, the number of intact pyramidal cells was markedly elevated in the treated mice, and this effect was blocked by F. Immunohistochemistry and Western blot analysis showed that the expression levels of NMDAR1, SYN, and p-CREC/CREB protein levels were significantly increased in the T group, while F inhibited the T-mediated effects. Western blot analysis showed that there were no significant differences in the expression levels of SYN, p-CREC/CREB, and NMDAR1 between C, F, and F + T groups. Reverse-transcription polymerase chain reaction (RT-PCR) analysis showed that the mRNA expression levels of NMDAR1 and SYN were significantly increased in T-administered mice, while F inhibited the T-mediated effects. Data suggest that the T-mediated increase in SYN expression levels resulted in improvement in behavioral performances and learning, which may involve stimulation of central nervous system androgen receptors (AR).
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2015 |
Huo DS, Zhang M, Cai ZP, Dong CX, Wang H, Yang ZJ, 'The Role of Nerve Growth Factor in Ginsenoside Rg1-Induced Regeneration of Injured Rat Sciatic Nerve', Journal of Toxicology and Environmental Health - Part A: Current Issues, 78 1328-1337 (2015) [C1] Sciatic nerve injury is commonly seen in clinical practice predominantly associated with trauma or sports injuries. Recent studies indicated that ginsenoside Rg1 (Gs Rg1), extract... [more] Sciatic nerve injury is commonly seen in clinical practice predominantly associated with trauma or sports injuries. Recent studies indicated that ginsenoside Rg1 (Gs Rg1), extracted from Chinese herbs, was found to promote regeneration of injured rat sciatic nerve and that nerve growth factor (NGF) may be involved in this process. The aim of this study was to examine the role that NGF may play in ginsenoside Rg1-induced regeneration of rat sciatic nerve following injury. Animals following surgical right sciatic nerve injury were subsequently administered intraperitoneally either saline (sham control) or different doses of 2, 4, 8, or 12 mg/kg daily GsRg1 for 2 to 8 wk. In addition, 100 g/kg mecobalamin, a drug utilized to treat nerve injuries, was employed as a positive control. After 2, 4, or 8 wk, sciatic functional index (SFI) and mean nerve conduction velocity (MNCV), markers of sciatic nerve function, were assessed to determine whether recovery of injured sciatic nerve occurred. In addition, immunohistochemistry and Western blot methods were used to examine NGF protein expression changes. Results showed that all doses of GsRg1 significantly increased SFI and MNCV in injured sciatic-nerve-damaged rats in a manner similar to that noted with mecobalamin. It is of interest that the intermediate 4- and 8-mg/kg doses were more effective in restoring nerve functions. Immunohistochemistry and Western blot results also demonstrated a similar pattern with enhanced NGF protein expression at all doses, but greater effects were noted at 4 and 8 mg/kg GsRg1. Data suggest that GsRg1 promotes recovery of injured sciatic nerve functions within a specific dose range and that NGF may be involved in this physiological process.
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2015 |
Wang C, Leigh J, Wang S, Wang H, 'Intervention of pentoxifylline on silica-induced inflammatory reaction and apoptosis', TOXICOLOGICAL AND ENVIRONMENTAL CHEMISTRY, 97 640-650 (2015) [C1]
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2014 |
Sun Y, Xu L, Shao H, Wang H, 'China's laws, rights, and administrative structures in occupational safety and health: A comparison with the United States', JOURNAL OF PUBLIC HEALTH POLICY, 35 455-469 (2014)
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2013 |
Yadav S, Anbalagan M, Shi Y, Wang F, Wang H, 'Arsenic inhibits the adipogenic differentiation of mesenchymal stem cells by down-regulating peroxisome proliferator-activated receptor gamma and CCAAT enhancer-binding proteins', TOXICOLOGY IN VITRO, 27 211-219 (2013)
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2013 |
Shi Y, Roy-Engel AM, Wang H, 'Effects of Corexit Dispersants on Cytotoxicity Parameters in a Cultured Human Bronchial Airway Cells, BEAS-2B', JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A-CURRENT ISSUES, 76 827-835 (2013)
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2013 |
Wang H, Wang JJ, Sanderson BJS, 'IN VITRO ADVERSE EFFECTS OF IRON ORE DUSTS ON HUMAN LYMPHOBLASTOID CELLS IN CULTURE', JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A-CURRENT ISSUES, 76 874-882 (2013)
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2012 |
Major DN, Wang H, 'How public health impact is addressed: a retrospective view on three different oil spills', TOXICOLOGICAL AND ENVIRONMENTAL CHEMISTRY, 94 442-467 (2012)
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2012 |
Major D, Zhang Q, Wang G, Wang H, 'Oil-dispersant mixtures: understanding chemical composition and its relation to human toxicity', TOXICOLOGICAL AND ENVIRONMENTAL CHEMISTRY, 94 1832-1845 (2012)
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2012 |
Wang H, Shi Y, Major D, Yang Z, 'Lung epithelial cell death induced by oil-dispersant mixtures', TOXICOLOGY IN VITRO, 26 746-751 (2012)
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2011 |
Yang ZJ, Song F, Wang ZJ, Shi Y, Fang G, Wang H, 'Co-administration of Mixed Steroid Hormones can Enhance the Recovery of Spermatogenesis Damaged by Gossypol Acetic Acid in Adult Rats', Journal of Reproduction and Contraception, 22 233-245 (2011) Objective: To determine whether co-administration of mixed steriod hormones can enhance the restoration of spermatogenesis damaged by gossypol acetic acid (GA). Methods: Adult mal... [more] Objective: To determine whether co-administration of mixed steriod hormones can enhance the restoration of spermatogenesis damaged by gossypol acetic acid (GA). Methods: Adult male Wistar rats were treated daily for 8 weeks with GA at 50mg/kg plus testosterone undercanoate (100 mg/kg)/desogestrel (0.125 mg/kg)/mini-dose ethinylestradiol (0.025 mg/kg) (TU/DSG/EE), followed by a period of 9 weeks for recovery. Control animals were administered the same dose of GA or TU/DSG/EE, and vehicle, respectively. Testis weight, testicular sperm head count and histological analysis were utilized to assess the spermatogenesis. Results: At the end of the 9-week reovery period, in rats given GA alone, spermatogenesis steadily declined. However, when rats received combined hormone administration during GA treatment, this decline was prevented and an complete recovery of spermagenesis occurred. The haploid spermatids and spermatocytes was not to be protected but to be more aggravatedly damaged. The excellent recovery must have resulted from that the hormone treatment could protect the ability of stem spermatogonia to differentialte and evolve progressively into mature spermatozoa. In addition, the concentrations of serum LH, FSH and intratesticular testosteron (ITT) notably decreased after 2 or 8 weeks of treatment, then returned to control levels at the end of 9-week recovery period. Conclusion: Steriod hormone treatment enhaces the recovery of spermatogenesis through preventing seminiferous epithelim from GA-induced destructive damage in rats. The enhanced recovery was closely associated with the marked suppression in intratesticular testosteron (ITT). © 2011 The Editorial Board of Journal of Reproduction and Contraception.
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2010 |
Yadav S, Shi Y, Wang F, Wang H, 'Arsenite induces apoptosis in human mesenchymal stem cells by altering Bc1-2 family proteins and by activating intrinsic pathway', TOXICOLOGY AND APPLIED PHARMACOLOGY, 244 263-272 (2010)
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2010 |
Wang F, Shi Y, Yadav S, Wang H, 'p52-Bcl3 complex promotes cyclin D1 expression in BEAS-2B cells in response to low concentration arsenite', TOXICOLOGY, 273 12-18 (2010)
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2010 |
Shi Y, Wang F, He J, Yadav S, Wang H, 'Titanium dioxide nanoparticles cause apoptosis in BEAS-2B cells through the caspase 8/t-Bid-independent mitochondrial pathway', TOXICOLOGY LETTERS, 196 21-27 (2010)
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2010 |
Shi Y, Yadav S, Wang F, Wang H, 'Endotoxin Promotes Adverse Effects of Amorphous Silica Nanoparticles on Lung Epithelial Cells in Vitro', JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A-CURRENT ISSUES, 73 748-756 (2010)
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2010 |
Yadav S, Shi Y, Wang H, 'IL-16 effects on A549 lung epithelial cells: Dependence on CD9 as an IL-16 receptor?', JOURNAL OF IMMUNOTOXICOLOGY, 7 183-193 (2010)
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2007 |
Wang JJ, Sanderson BJS, Wang H, 'Cytotoxicity and genotoxicity of ultrafine crystalline SiO2 particulate in cultured human lymphoblastoid cells', ENVIRONMENTAL AND MOLECULAR MUTAGENESIS, 48 151-157 (2007)
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2007 |
Wang JJ, Sanderson BJS, Wang H, 'Cyto- and genotoxicity of ultrafine TiO2 particles in cultured human lymphoblastoid cells', MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS, 628 99-106 (2007)
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2007 |
Sanderson BJS, Wang JJ, Wang H, 'Letter and new data in response to Letter to the Editor by William P. Gulledge about article "Cyto- and genotoxicity of ultrafine TiO2 particles in cultured human lymphoblastoid cells" by Wang et al. Reply', MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS, 634 243-244 (2007)
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2007 |
Wang JJ, Wang H, Sanderson BJS, 'Ultrafine quartz-induced damage in human lymphoblastoid cells in vitro using three genetic damage end-points', TOXICOLOGY MECHANISMS AND METHODS, 17 223-232 (2007)
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2006 |
Wang H, Leigh J, 'Effects of nitric oxide synthase inhibitor -Nitro-L-Arginine Methyl Ester, on silica-induced inflammatory reaction and apoptosis', Particle and Fibre Toxicology, 3 (2006) Background: Although nitric oxide is overproduced by macrophages and neutrophils after exposure to silica, its role in silica-induced inflammatory reaction and apoptosis needs fur... [more] Background: Although nitric oxide is overproduced by macrophages and neutrophils after exposure to silica, its role in silica-induced inflammatory reaction and apoptosis needs further clarification. In this study, rats were intratracheally instilled with either silica suspension or saline to examine inflammatory reactions and intraperitoneally injected with ¿-nitro-L-arginine methyl ester (L-NAME), an inhibitor of nitric oxide synthases, or saline to examine the possible role of nitric oxide production in the reaction. Results: Results showed that silica instillation induced a strong inflammatory reaction indicated by increased total cell number, number of neutrophils, protein concentration and lactate dehydrogenase (LDH) activity in bronchoalveolar lavage fluid (BALF). There were no significant differences in these indices between silica-instilled groups with and without L-NAME injection (p > 0.05) except LDH level. The results also showed that apoptotic leucocytes were identified in BALF cells of silica-instilled groups whereas no significant difference was found between silica-instilled groups with and without L-NAME injection in the apoptotic reaction (p > 0.05). Silica instillation significantly increased the level of BALF nitrite/nitrate and L-NAME injection reduced this increase. Conclusion: Intratracheal instillation of silica caused an obvious inflammatory reaction and leucocyte apoptosis, but these reactions were not influenced by intraperitoneal injection of L-NAME and reduced production of NO. This supports the possibility that silica-induced lung inflammation and BALF cell apoptosis are via NO-independent mechanisms. © 2006 Wang and Leigh; licensee BioMed Central Ltd.
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2006 |
Loughran-Fowlds A, Oei J, Wang H, Xu H, Wimalasundera N, Egan C, et al., 'The influence of gestation and mechanical ventilation on serum Clara cell secretory protein (CC10) concentrations in ventilated and nonventilated newborn infants', PEDIATRIC RESEARCH, 60 103-108 (2006)
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2005 |
Thomas PS, Gibson PG, Wang H, Shah S, Henry RL, 'The relationship of exhaled nitric oxide to airway inflammation and responsiveness in children', Journal of Asthma, 42 291-295 (2005) [C1]
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2003 |
Gibson PG, Henry RL, Shah S, Powell H, Wang H, 'Migration to a Western Country Increases Asthma Symptoms But Not Eosinophilic Airway Inflammation', Pediatric Pulmonology, 36 209-215 (2003) [C1]
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2003 |
Oei J, Lui K, Wang H, Henry R, 'Decreased neutrophil apoptosis in tracheal fluids of preterm infants at risk of chronic lung disease', ARCHIVES OF DISEASE IN CHILDHOOD-FETAL AND NEONATAL EDITION, 88 245-249 (2003)
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2002 |
Dakin CJ, Numa AH, Wang H, Morton JR, Vertzyas CC, Henry RL, 'Inflammation, infection, and pulmonary function in infants and young children with cystic fibrosis', American Journal of Respiratory and Critical Care Medicine, 165 904-910 (2002) Our aim was to study the effect of lower airway infection on clinical parameters, pulmonary function tests, and inflammation in clinically stable infants and young children with c... [more] Our aim was to study the effect of lower airway infection on clinical parameters, pulmonary function tests, and inflammation in clinically stable infants and young children with cystic fibrosis (CF). To accomplish this goal, a prospective cohort of screened CF patients under 4 years of age were studied, using elective anesthesia and intubation for: passive respiratory mechanics (single breath occlusion passive deflation) and lung volumes (nitrogen washout), under neuromuscular blockade; and bronchoalveolar lavage (BAL) of 3 main bronchi for cytology, cytokine interleukin (IL)-8, and quantitative microbiology. There were 22 children studied, with a mean age of 23.2 months (6.7-44 months). A greater relative risk of lower airway pathogens was associated with prior respiratory admission (3.60, 95% confidence interval [Cl] 2.87-4.51), history of asthma (1.75, 95% Cl 1.52-2.03), and chronic symptoms (1,50, 95% Cl 1.23-1.83), especially wheeze (1.88, 95% Cl 1.61-2.19). Lower respiratory pathogens (= 10 cfu/ml BAL) were found in 14 out of 22, and greater than 105 cfu/ml in 8 out of 22 subjects. The level of pathogens in BAL (log10 cfu/ml) explained 78% of the variability in percent neutrophils and 34% of the variability in IL-8 levels. Pathogen level also correlated with pulmonary function tests of specific respiratory system compliance (r -0.49, p = 0.02) and functional residual capacity over total lung capacity (r 0.49, p = 0.03). We conclude that the presence of pathogens in the lower airways correlated with levels of inflammation, respiratory system compliance, and degree of air trapping.
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2002 |
Wang H, Oei J, Lui K, Henry R, 'Interleukin-16 in tracheal aspirate fluids of newborn infants', EARLY HUMAN DEVELOPMENT, 67 79-86 (2002)
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2002 |
Oei J, Lui K, Wang H, Henry R, 'Decreased interleukin-10 in tracheal aspirates from preterm infants developing chronic lung disease', ACTA PAEDIATRICA, 91 1194-1199 (2002)
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2002 |
Dakin CJ, Numa AH, Wang H, Morton JR, Vertzyas CC, Henry RL, 'Inflammation, infection, and pulmonary function in infants and young children with cystic fibrosis', AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, 165 904-910 (2002)
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2002 |
Dakin CJ, Pereira JK, Henry RL, Wang H, Morton JR, 'Relationship between sputum inflammatory markers, lung function, and lung pathology on high-resolution computed tomography in children with cystic fibrosis', PEDIATRIC PULMONOLOGY, 33 475-482 (2002)
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2001 |
Wang H, 'Interlel'kin-16 in trachea aspirates of preterm infants with chronic lung disease', Respirology, 6 (2001) Current views on pathogenesis of chronic lung disease (OLD) support an imbalance of inflammatory and healing processes in susceptible premature infants. Persistence of proinflamma... [more] Current views on pathogenesis of chronic lung disease (OLD) support an imbalance of inflammatory and healing processes in susceptible premature infants. Persistence of proinflammatory cytokines such as IL-6. IL-8 and TNF-lalpa in trachéal aspirate fluide (TAP) could be a marker of CLD. However, their use in early prediction of Cl.D development is limited. Over the last two decades, systemic dexamethasone have been used in an attempt to attenuate this chronic inflammatory process in CLD infants. Corticosteroids inhibit transcription of some proinflammatory cytokines. It has been demonstrated that dexamethasone can suppress the production of IL-16.11.-16 is a chemoattractant of CD4 cells including lymphocytes and monocytes. Lymphocytes and macrophages transformed from monocytes are more frequently encountered in persistent lung inflammation than acute inflammation. Thus, we hypothesized that IL-16 may play a role in the persistence of lung inflammation in the development of CLD. Fifty-five TAP samples obtained from mechanically ventilated preterm infants during the first 4 weeks of life were analysed by EL1SA kit method (R&D). Among the 17 samples from preterm NON-CLD infants. IL-16 was undetectable. Among the 38 samples from CLD infants, 15 samples were detected (39.5%). The IL-16 range was 900-42100 pg'ml. Considerable proportion of CLD infants ere treated with dexamethasone, both the IL-16 levels and the positive rate may have been affected. Based on the above preliminary result, we concluded that IL-16 is detectable in preterm infants suffered from CLD. Further analysis and studies particularly regarding the influence of corticosteroids treatment are needed. |
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1999 |
Dakin C, Wang H, Morton J, Henry R, 'Correlation between sputum cytology in cystic fibrosis and response to treatment of acute exacerbation', Respirology, 4 (1999) In cystic fibrosis (CF) changes in pulmonary infection or inflammation should be reflected in sputum cytology, with variability between samples being explained by the disease proc... [more] In cystic fibrosis (CF) changes in pulmonary infection or inflammation should be reflected in sputum cytology, with variability between samples being explained by the disease process. Original sputum processing techniques may fail to show a change due to specimen cell clumping. Method: Prospective study of children with CF admitted to hospital with an exacerbation of chest infection, from July to October 1998. Spontaneous sputum samples were collected during admission. Sputum processing was selective, with dispersion by the 3 enzyme method. Specimens were discarded if >3% squamous cells. Other variables recorded included demographic, day of admission and FEVi. Results: There were 10 children studied (5 males) with mean age 12.1 years (8.1-19.1). Mean admission length was 13 days (4-20). 30 specimens were processed, with >2 values for 5/10 children. FEVi mean at admission was 52% predicted (25-72%), and at discharge 58% (29-93%). Sputum cytology total cell count (TCC) mean was 34.58 x106 (+/- 38.33), with neutrophil mean 33.21 x 10 (+/- 37.77). In 9/10 children the TCC varied inversely with FEVi. In 5/10 the TCC decreased > 2x and FEVi increased >10%, in 3/10 neither TCC nor FEVi changed, and in 1/10 TCC increased and FEVi decreased. Absolute values of FEVi correlated negatively with TCC (-0.54, p=0.01). Conclusion: Absolute values of sputum TCC were useful in reflecting response to treatment during admission. Not every patient had a change in TCC. Using this sputum processing method, there was a significant correlation between sputum TCC and lung function. |
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Show 72 more journal articles |
Conference (7 outputs)
Year | Citation | Altmetrics | Link | |||||
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2018 | Palaniappan K, Ramasoori Krishnan SP, James C, Wang H, 'Nano Titanium Dioxide (TiO2) Enabled Products in Singapore: Results of a Mapping Study', MIHAICE 2018 Program Book, Kuala Lumpur, Malaysia (2018) | |||||||
2018 | Holland J, Wang H, James C, 'CAN THE SMARTCAP (R) ALARM RATE PREDICT FATIGUE RELATED MEDICAL CONDITIONS?', Can the Smartcap® alarm rate predict fatigue related medical conditions?, 14 16 May 2018 Sydney (2018) | |||||||
2015 | Liu X, Pisaniello D, Sanderson B, Wang H, 'CYTOTOXICITY AND GENOTOXICITY OF ULTRAFINE IRON-ORE DUST', RESPIROLOGY, Queensland, AUSTRALIA (2015) | |||||||
2010 |
Liu X, Salter A, Thomas P, Leigh J, Wang H, 'EXHALED NITRIC OXIDE LEVELS AND LUNG FUNCTION CHANGES OF UNDERGROUND COAL MINERS IN NEWCASTLE, AUSTRALIA', JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A-CURRENT ISSUES, Cape Town, SOUTH AFRICA (2010)
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2004 | Louhgran-Fowlds AS, Oei J, Wimalasunderera N, Wang H, Xu HX, An C, et al., 'The influence of gestation and mechanical ventilation on serum Clara Cell Protein in preterm infants', PEDIATRIC RESEARCH, San Francisco, CA (2004) | |||||||
2000 |
Leigh J, Bonin A, Wang H, 'In vivo genotoxicity of crystalline silica as evidenced by micronuclei in pulmonary alveolar macrophages: Low-dose study', INHALATION TOXICOLOGY, MAASTRICHT, NETHERLANDS (2000)
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Show 4 more conferences |
Research Supervision
Number of supervisions
Past Supervision
Year | Level of Study | Research Title | Program | Supervisor Type |
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2022 | PhD | The Ligand Docking Experiments between Olfactory Receptors and Allergen Molecules and the Classification of Olfactory Receptors using Machine Learning Methods | PhD (Environ & Occupat Hlth), College of Health, Medicine and Wellbeing, The University of Newcastle | Principal Supervisor |
2020 | PhD | The Implication of Autophagy in Cell Fate of Human Bone Marrow-Derived Mesenchymal Stem Cells Exposed to Titanium Dioxide Nanoparticles | PhD (Environ & Occupat Hlth), College of Health, Medicine and Wellbeing, The University of Newcastle | Principal Supervisor |
Dr Harry Wang
Position
Honorary Senior Lecturer
Chronic Respiratory Diseases
School of Health Sciences
College of Health, Medicine and Wellbeing
Contact Details
he.wang@newcastle.edu.au | |
Phone | (02) 4921 7735 |
Fax | (02) 4921 7053 |
Office
Room | HA08 |
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Building | Hunter Building |
Location | Callaghan University Drive Callaghan, NSW 2308 Australia |