Dr  Feng Shi

Dr Feng Shi

Senior Research Fellow

Global Centre for Environmental Remediation

Career Summary

Biography

Dr Feng is currently working at a Senior Research Fellow at Global Centre for Environmental Remediation (GCER), University of Newcastle, Australia. His post-graduate studies based at Cawthron Institute (Nelson, New Zealand) involve investigation of novel toxins produced by the marine dinoflagellate Karenia brevisulcata. He was awarded a PhD in Toxicology by Lincoln University, New Zealand in 2012. Dr Feng Shi joined work as chemist/senior chemist in 2013 in Sydney and had been managing a NATA ISO/IEC 17025:2017 accredited laboratory in Sydney, performing food chemistry, biotoxin and environmental analysis before joint GCER in 2020. He is a NATA appointed technical assessor for biotoxin and environmental contaminant analysis in Australia. His research background and experience is varied and covers a range of disciplines in the general area of analytical chemistry, natural compound chemistry, toxicology and environmental science:
  • Natural compound chemistry - extraction, isolation and chromatographic purification of novel compounds; spectroscopic characterization;
  • Analytical chemistry – method development and validation using LC-HRMS, LC-MS/MS, GC-HRMS and GC-MS/MS;
  • Toxicology - fish and larval bioassays; mammalian cell assays including mode of action studies (sodium channel; apoptosis);
  • Environmental assessment and monitoring: NEPM 2013, Dredging 2009, ANZECC & ARMCANZ 2000;
  • Laboratory management and good QA/QC practice including ISO17025, APVMA, and FDA.

Qualifications

  • Doctor of Philosophy, Lincoln University - New Zealand
  • Postgraduate Diploma in Applied Science, Lincoln University - New Zealand

Keywords

  • Analytical Chemistry
  • Food Safety
  • Instrumental Analysis
  • Laboratory Management
  • Natural Compound Chemistry
  • Quality Assurance
  • Toxicology

Languages

  • English (Working)

Fields of Research

Code Description Percentage
340105 Instrumental methods (excl. immunological and bioassay methods) 40
340502 Natural products and bioactive compounds 30
410402 Environmental assessment and monitoring 30

Professional Experience

UON Appointment

Title Organisation / Department
Senior Research Fellow University of Newcastle
Global Centre for Environmental Remediation
Australia

Professional appointment

Dates Title Organisation / Department
11/12/2017 - 11/5/2020 Manager Symbio Laboratories
Sydney Chemistry Laboratory
Australia
4/3/2013 - 11/12/2017 Senior Chemist/Chemist Advanced Analytical Australia Ltd
Australia
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Publications

For publications that are currently unpublished or in-press, details are shown in italics.


Journal article (15 outputs)

Year Citation Altmetrics Link
2024 Liu S, Duan L, Shi F, Filippelli GM, Naidu R, 'Concentrations of per- and polyfluoroalkyl substances in vegetables from Sydney and Newcastle, Australia.', J Sci Food Agric, (2024)
DOI 10.1002/jsfa.13491
Co-authors Ravi Naidu
2023 Al Amin M, Luo Y, Shi F, Yu L, Liu Y, Nolan A, et al., 'A modified TOP assay to detect per- and polyfluoroalkyl substances in aqueous film-forming foams (AFFF) and soil.', Front Chem, 11 1141182 (2023) [C1]
DOI 10.3389/fchem.2023.1141182
Co-authors Ravi Naidu, Megh Mallavarapu, Yanju Liu, Cheng Fang
2021 Satake M, Irie R, Holland PT, Harwood DT, Shi F, Itoh Y, et al., 'Brevisulcenals-A1 and A2, Sulfate Esters of Brevisulcenals, Isolated from the Red Tide Dinoflagellate Karenia brevisulcata', Toxins, 13 (2021) [C1]

Two different types of polycyclic ether toxins, namely brevisulcenals (KBTs) and brevisulcatic acids (BSXs), produced by the red tide dinoflagellate Karenia brevisulcata, were the... [more]

Two different types of polycyclic ether toxins, namely brevisulcenals (KBTs) and brevisulcatic acids (BSXs), produced by the red tide dinoflagellate Karenia brevisulcata, were the cause of a toxic incident that occurred in New Zealand in 1998. Four major components, KBT-F,-G,-H, and-I, shown to be cytotoxic and lethal in mice, were isolated from cultured K. brevisulcata cells, and their structures were elucidated by spectroscopic analyses. New analogues, brevisulcenal-A1 (KBT-A1) and brevisulcenal-A2 (KBT-A2), toxins of higher polarity than that of known KBTs, were isolated from neutral lipophilic extracts of bulk dinoflagellate culture extracts. The structures of KBT-A1 and KBT-A2 were elucidated as sulfated analogues of KBT-F and KBT-G, respectively, by NMR and matrix-assisted laser desorption/ionization tandem mass spectrometry (MALDI TOF/TOF), and by comparison with the spectra of KBT-F and KBT-G. The cytotoxicities of the sulfate analogues were lower than those of KBT-F and KBT-G.

DOI 10.3390/TOXINS13020082
Citations Scopus - 3Web of Science - 2
2021 Liu W, Su Y, Liu J, Zhang K, Wang X, Chen Y, et al., 'Determination of cyflufenamid residues in 12 foodstuffs by QuEChERS-HPLC-MS/MS', Food Chemistry, 362 (2021) [C1]
DOI 10.1016/j.foodchem.2021.130148
Citations Scopus - 21Web of Science - 11
2021 Wu X, Cai Y, Shi F, Duan L, Zhang Q, Xu N, 'Characterization of allelopathic compounds from the harmful dinoflagellate, Cochlodinium geminatum', Harmful Algae, 107 (2021) [C1]

In 2006, species of Cochlodinium geminatum were first recorded as new, harmful algal bloom (HAB)-forming dinoflagellate, in the Pearl River Estuary, China. Previous studies indica... [more]

In 2006, species of Cochlodinium geminatum were first recorded as new, harmful algal bloom (HAB)-forming dinoflagellate, in the Pearl River Estuary, China. Previous studies indicated that allelopathy (the release of chemical compounds) is an important competitive strategy for C. geminatum in inhibiting the growth of phytoplankton competitors, however, little is known about the allelochemicals produced by this species. In this study, allelopathic compounds were characterized using a series of chromatographic techniques. Methanol extracts from cultures showed significant inhibitory effects towards the co-occurring phytoplankton, Akashiwo sanguinea, and potent hemolytic activity against rabbit erythrocytes. Solid phase extraction (SPE) fractions from a reverse phase SPE column, exhibited inhibitory and synergistic effects on A. sanguinea growth, of which fractions eluted in 80% and 100% methanol showed the strongest allelopathic activity. These fractions were further purified using reverse phase high performance liquid chromatography (HPLC), and six fractions were collected. Three fractions displayed strong inhibitory effects towards A. sanguinea, with 14%¿25% inhibitory rates, but a higher 35% inhibitory rate was observed when fractions were combined, suggesting allelochemicals had synergistic allelopathic effects. Allelopathic activity also exhibited time dependent reduction when stored at -20°C. Furthermore, trypsin digestion and dialysis assays indicated C. geminatum produced multiple allelopathic compounds. Our study demonstrated that species of C. geminatum produced and released multiple allelopathic compounds of low polarity and sufficient hydrophilicity, which elicit important competitive advantages by inhibiting co-occurring phytoplankton, and promoting population growth.

DOI 10.1016/j.hal.2021.102069
Citations Scopus - 2
2018 Satake M, Irie R, Hamamoto Y, Tachibana K, Holland PT, Harwood DT, et al., 'BREVISULCENAL-G, -H, and -I, POLYCYCLIC ETHER MARINE TOXINS FROM THE DINOFLAGELLATE KARENIA BREVISULCATA', HETEROCYCLES, 96 2096-2105 (2018)
DOI 10.3987/COM-18-14014
Citations Scopus - 3Web of Science - 3
2018 Dorantes-Aranda JJ, Tan JYC, Hallegraeff GM, Campbell K, Ugalde SC, Harwood DT, et al., 'Detection of Paralytic Shellfish Toxins in Mussels and Oysters Using the Qualitative Neogen Lateral-Flow Immunoassay: An Interlaboratory Study', JOURNAL OF AOAC INTERNATIONAL, 101 468-479 (2018)
DOI 10.5740/jaoacint.17-0221
Citations Web of Science - 9
2016 Irie R, Suzuki R, Tachibana K, Holland PT, Harwood DT, Shi F, et al., 'BREVISULCATIC ACIDS FROM A MARINE MICROALGAL SPECIES IMPLICATED IN A TOXIC EVENT IN NEW ZEALAND', HETEROCYCLES, 92 45-54 (2016)
DOI 10.3987/COM-15-13332
Citations Scopus - 6Web of Science - 5
2014 Harwood DT, Shi F, Satake M, Holland PT, 'A sensitive LC-MS/MS assay for brevisulcenal and brevisulcatic acid toxins produced by the dinoflagellate Karenia brevisulcata', TOXICON, 84 19-27 (2014)
DOI 10.1016/j.toxicon.2014.03.004
Citations Scopus - 10Web of Science - 10
2014 Suzuki R, Irie R, Harntaweesup Y, Tachibana K, Holland PT, Harwood DT, et al., 'Brevisulcatic Acids, Marine Ladder-Frame Polyethers from the Red Tide Dinoflagellate Karenia brevisulcata in New Zealand', ORGANIC LETTERS, 16 5850-5853 (2014)
DOI 10.1021/ol502700h
Citations Scopus - 13Web of Science - 11
2013 Selwood AI, Wilkins AL, Munday R, Shi F, Rhodes LL, Holland PT, 'Portimine: a bioactive metabolite from the benthic dinoflagellate Vulcanodinium rugosum', TETRAHEDRON LETTERS, 54 4705-4707 (2013)
DOI 10.1016/j.tetlet.2013.06.098
Citations Scopus - 63Web of Science - 49
2012 Hamamoto Y, Tachibana K, Holland PT, Shi F, Beuzenberg V, Itoh Y, Satake M, 'Brevisulcenal-F: A Polycyclic Ether Toxin Associated with Massive Fish-kills in New Zealand', JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 134 4963-4968 (2012)
DOI 10.1021/ja212116q
Citations Scopus - 43Web of Science - 40
2012 Shi F, McNabb P, Rhodes L, Holland P, Webb S, Adamson J, et al., 'The toxic effects of three dinoflagellate species from the genus Karenia on invertebrate larvae and finfish', NEW ZEALAND JOURNAL OF MARINE AND FRESHWATER RESEARCH, 46 149-165 (2012)
DOI 10.1080/00288330.2011.616210
Citations Scopus - 31Web of Science - 29
2012 Beuzenberg V, Mountfort D, Holland P, Shi F, MacKenzie L, 'Optimization of growth and production of toxins by three dinoflagellates in photobioreactor cultures', JOURNAL OF APPLIED PHYCOLOGY, 24 1023-1033 (2012)
DOI 10.1007/s10811-011-9726-8
Citations Scopus - 21Web of Science - 16
2012 Holland PT, Shi F, Satake M, Hamamoto Y, Ito E, Beuzenberg V, et al., 'Novel toxins produced by the dinoflagellate Karenia brevisulcata', HARMFUL ALGAE, 13 47-57 (2012)
DOI 10.1016/j.hal.2011.10.002
Citations Scopus - 33Web of Science - 28
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Research Supervision

Number of supervisions

Completed0
Current1

Current Supervision

Commenced Level of Study Research Title Program Supervisor Type
2023 PhD Assessment of the Environment Contaminants in Selected Environment Samples by Multi Residue Method using Quadrupole Time of Flight Mass Spectrometry PhD (Environment Remediation), College of Engineering, Science and Environment, The University of Newcastle Co-Supervisor
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Dr Feng Shi

Position

Senior Research Fellow
Global Centre for Environmental Remediation
College of Engineering, Science and Environment

Contact Details

Email feng.shi@newcastle.edu.au
Link Personal webpage
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