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Associate Professor Christopher Scarlett

Associate Professor

School of Environmental and Life Sciences (Applied Sciences)

Career Summary

Biography

I undertook my Bachelor of Science (Hons I) studies at the University of Newcastle. I then completed my PhD through the University of Sydney, Faculty of Medicine where I established a novel proteomic initiative with the specific aim of using proteomics as a tool to answer clinically relevant questions. Using this approach I identified unique markers of pancreatic and bile duct cancer by creating powerful new diagnostic models for the improved diagnosis of pancreatico-biliary malignancies. I then continued my interest in pancreatic cancer research by joining the Pancreatic Cancer Research Group at the Garvan Institute of Medical Research in 2007.

In my early post-doctoral years, I was awarded an NHMRC Postdoctoral Training Fellowship (Peter Doherty) and was funded as a Cancer Institute NSW Early Career Development Fellow at the Garvan Institute. My research interests primarily focussed on signalling pathways in pancreatic cancer to identify novel therapeutic and chemopreventative strategies, as well as defining the role of bone marrow derived cells in the development of the normal pancreas, pancreatic injury and regeneration, and pancreatic cancer.

Recognition of these contributions included a feature article in the CINSW Cancer Research Achievement Report 2008; A celebration of the outstanding contributions made to cancer research in NSW. I am also is a Cancer Institute NSW Career Development Fellow investigating novel therapeutic approaches to pancreatic cancer, and was awarding the Cancer Research Network Innovation Award (Sydney Cancer Conference 2010).

I am also investigating the personalised treatment of metastatic pancreatic cancer based on genomic sequence data acquired through the Australian Pancreatic Cancer Genome Initiative, as part of the International Cancer Genome Consortium.

I moved to the University of Newcastle in early 2012 to take a position within the School of Environmental and Life Sciences,and am now driving a research program investigating aberrations in nutrient-gene interactions to define clinically relevant phenotypes of pancreatic cancer, as well as examining the efficacy of bioactive compounds as novel therapeutics in the treatment of pancreatic cancer.

Research Expertise
My research interests have primarily focused on signalling pathways in pancreatic cancer to identify novel therapeutic and chemopreventative strategies, as well as defining the role of bone marrow derived cells in the development of the normal pancreas, pancreatic injury and regeneration, and pancreatic cancer.

I am also investigating novel therapeutic approaches to pancreatic cancer in addition to investigating personalised treatment of metastatic pancreatic cancer based on genomic sequence data acquired through the Australian Pancreatic Cancer Genome Initiative, as part of the International Cancer Genome Consortium (ICGC). Upon my move to the University of Newcastle, School of Environmental and Life Sciences, I have been investigating aberrations in nutrient-gene interactions to define clinically relevant phenotypes of pancreatic cancer, as well as examining the efficacy of bioactive compounds as novel therapeutics in the treatment of pancreatic cancer.

Teaching Expertise
I have been involved in coordinating and teaching core courses for the Bachelor of Food Science and Human Nutrition Program, in particular BIOL2011 - Fundamentals of Biology and Biochemistry; and FSHN2100 - Microbiology, Food Safety and Immunology.

Collaborations
I am an Associate Investigator for the Australian Pancreatic Cancer Network, based at the Garvan Institute of Medical Research, which provides direct links to the clinical management of patients facilitating rapid translation of scientific discoveries to patient care, providing a unique opportunity for translational research efforts in pancreatic cancer in Australia.

I am also contributing to the Australian Pancreatic Cancer Genome Initiative (APGI), which aims to sequence ~400 individual pancreatic cancers as part of the International Cancer Genome Consortium (ICGC).

I have been awarded project grant funding through the Priority-driven Collaborative Cancer Research Scheme, co-funded by Cancer Australia and the Cure Cancer Australia Foundation (CIA; 2012-1013). I am also a Co-Investigator on a successful NHMRC program grant ($11,128,320; 2009-2013), and a Co-Chief Investigator on successful project grants through the NHMRC (CI-B; 2011-2013) and The Cancer Council NSW (CI-C; 2010-2012) in collaboration with Dr Tao Liu at the Children’s Cancer Institute Australia.


Qualifications

  • PhD, University of Sydney
  • Bachelor of Science, University of Newcastle
  • Bachelor of Science (Honours), University of Newcastle

Keywords

  • Biochemistry
  • Carcinogenesis
  • Metastases
  • Microbiology
  • Mouse Models
  • Novel Therapeutics
  • Pancreatic Cancer
  • Research Methods

Fields of Research

Code Description Percentage
111299 Oncology and Carcinogenesis not elsewhere classified 60
111201 Cancer Cell Biology 20
111204 Cancer Therapy (excl. Chemotherapy and Radiation Therapy) 20

Professional Experience

UON Appointment

Title Organisation / Department
Associate Professor University of Newcastle
School of Environmental and Life Sciences
Australia

Academic appointment

Dates Title Organisation / Department
1/06/2012 - 1/02/2013 Fellow

Cancer Institute NSW:- Career Development Fellowship

University of Newcastle
School of Environmental and Life Sciences
Australia
1/03/2012 -  Honorary Scientist Garvan Institute of Medical Research
Cancer Research Program
Australia
1/01/2007 - 1/03/2012 Senior Research Officer Garvan Institute of Medical Research
Cancer Research Program
Australia
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Publications

For publications that are currently unpublished or in-press, details are shown in italics.


Book (2 outputs)

Year Citation Altmetrics Link
2015 Scarlett CS, Vuong QV, Plant Bioactive Compounds for Pancreatic Cancer Prevention and Treatment, Nova Science Publishers, Inc., New York (2015) [A3]
Co-authors Vanquan Vuong
2014 Scarlett CJ, Vuong QV, Preface (2014)
Co-authors Vanquan Vuong

Chapter (4 outputs)

Year Citation Altmetrics Link
2015 Vuong QV, Scarlett CJ, 'Selected Australian Flora As Potential Sources of Anti-Cancer Agents', Plant Bioactive Compounds for Pancreatic Cancer Prevention and Treatment, Nova Science Publishers , Inc., New York 195-217 (2015) [B1]
Co-authors Vanquan Vuong
2015 Bowyer MC, McCluskey A, Scarlett CJ, Vuong QV, 'Pancreatic Cancer drugs: Case Studies in Synthesis and Production', Plant Bioactive Compounds for Pancreatic Cancer Prevention and Treatment, Nova Science Publishers, New York 145-193 (2015) [B1]
Co-authors Vanquan Vuong, Adam Mccluskey, Michael Bowyer
2015 Thuong PT, Khoi NM, Scarlett CJ, Ito F, 'Vietnamese Medicinal Plants as Potential Anticancer Agents', Plant Bioactive Compounds for Pancreatic Cancer Prevention and Treatment, Nova Science Publishers, New York 219-252 (2015) [B1]
2013 Vuong QV, Scarlett CJ, Roach PD, 'Green tea and pancreatic cancer chemoprevention', Green Tea: Varieties, Production and Health Benefits, Nova Science Publishers, New York 75-90 (2013) [B1]
Citations Scopus - 1
Co-authors Paul Roach, Vanquan Vuong
Show 1 more chapter

Journal article (109 outputs)

Year Citation Altmetrics Link
2017 Van TN, Vuong QV, Bowyer MC, Van Altena IA, Scarlett CJ, 'MICROWAVE-ASSISTED EXTRACTION FOR SAPONINS AND ANTIOXIDANT CAPACITY FROM XAO TAM PHAN (PARAMIGNYA TRIMERA) ROOT', JOURNAL OF FOOD PROCESSING AND PRESERVATION, 41 (2017) [C1]
DOI 10.1111/jfpp.12851
Citations Web of Science - 2
Co-authors Ian Vanaltena, Vanquan Vuong, Michael Bowyer
2017 Saberi B, Thakur R, Bhuyan DJ, Vuong QV, Chockchaisawasdee S, Golding JB, et al., 'Development of edible blend films with good mechanical and barrier properties from pea starch and guar gum', STARCH-STARKE, 69 (2017) [C1]
DOI 10.1002/star.201600227
Citations Scopus - 2
Co-authors Vanquan Vuong
2017 Bhuyan DJ, Vuong QV, Chalmers AC, van Altena IA, Bowyer MC, Scarlett CJ, 'Development of the ultrasonic conditions as an advanced technique for extraction of phenolic compounds from Eucalyptus robusta', SEPARATION SCIENCE AND TECHNOLOGY, 52 100-112 (2017) [C1]
DOI 10.1080/01496395.2016.1250777
Citations Scopus - 1Web of Science - 1
Co-authors Ian Vanaltena, Vanquan Vuong, Michael Bowyer, Anita Chalmers
2017 Vu HT, Scarlett CJ, Vuong QV, 'Optimization of ultrasound-assisted extraction conditions for recovery of phenolic compounds and antioxidant capacity from banana (Musa cavendish) peel', Journal of Food Processing and Preservation, 41 (2017)

© 2016 Wiley Periodicals, Inc. A large quantity of banana peel is generated annually and is considered as waste with low value. This study aimed to optimize five extraction param... [more]

© 2016 Wiley Periodicals, Inc. A large quantity of banana peel is generated annually and is considered as waste with low value. This study aimed to optimize five extraction parameters; including ultrasonic temperature, time and power, as well as acetone concentration and sample to solvent ratio, for maximum recovery of phenolic compounds, flavonoids, proanthocyanidins and antioxidant properties from banana (Musa cavendish) peel using response surface methodology. The results showed that recovery yields of phenolic compounds, flavonoids, proanthocyanidins and antioxidant properties were affected by the extraction parameters; of which the acetone concentration had the greatest effect. Optimal extraction conditions were found to be at ultrasonic temperature of 30°C, ultrasonic time of 5 min, ultrasonic power of 150 W, sample to solvent ratio of 8:100 g/mL and acetone concentration of 60%. Under these optimal conditions, 23.49 mg of phenolic compounds, 39.46 mg of flavonoids and 13.11 mg of proanthocyanidins could be extracted from 1 g of banana (M. cavendish) peel. Practical applications: Banana peel known as waste is generated in a big quantity with limited utilization. Therefore, it is necessary to utilize this by-product for adding value to food industry. This study was designed to establish a simple, effective extraction method for maximum recovery of phenolic compounds from banana peel. Findings from this study can be used for further isolation and purification of phenolic compounds from banana peel for subsequent application in nutraceutical and pharmaceutical industry.

DOI 10.1111/jfpp.13148
Co-authors Vanquan Vuong
2017 Saberi B, Vuong QV, Chockchaisawasdee S, Golding JB, Scarlett CJ, Stathopoulos CE, 'Physical, Barrier, and Antioxidant Properties of Pea Starch-Guar Gum Biocomposite Edible Films by Incorporation of Natural Plant Extracts', Food and Bioprocess Technology, 1-11 (2017)

© 2017 Springer Science+Business Media, LLC Active food packaging based on pea starch and guar gum (PSGG) films containing natural antioxidants (NAs) was developed. Four kinds of... [more]

© 2017 Springer Science+Business Media, LLC Active food packaging based on pea starch and guar gum (PSGG) films containing natural antioxidants (NAs) was developed. Four kinds of NAs (epigallocatechin gallate (EGCG), blueberry ash (BBA) fruit extract, macadamia (MAC) peel extract, and banana (BAN) peel extract) were added into the PSGG-based films as antioxidant additive. The effects of these compounds at different amounts on the physical and antioxidant characteristics of the PSGG film were investigated. The antioxidant activity was calculated with three analytical assays: DPPH radical scavenging ability assay, cupric reducing antioxidant capacity (CUPRAC), and ferric reducing activity power (FRAP). EGCG-PSGG films showed higher antioxidant activity, followed by BBA-PSGG, MAC-PSGG, and BAN-PSGG films, at all concentrations (0.75¿3 mg/mL) and with all procedures tested. Additionally, the antioxidant activity of films showed a concentration dependency. The results revealed that addition of NAs made the PSGG film darker and less transparent. However, the moisture barrier was significantly improved when NAs were incorporated into the film. The FTIR spectra were examined to determine the interactions between polymers and NAs. The results suggested that incorporation of EGCG, BBA, MAC, and BAN into PSGG films have great potential for use as active food packaging for food preservation.

DOI 10.1007/s11947-017-1995-z
Co-authors Vanquan Vuong
2017 Pristijono P, Bowyer MC, Scarlett CJ, Vuong QV, Stathopoulos CE, Golding JB, 'The application of low pressure storage to maintain the quality of zucchinis', New Zealand Journal of Crop and Horticultural Science, (2017)
DOI 10.1080/01140671.2017.1383277
Co-authors Vanquan Vuong, Michael Bowyer
2017 Ngoc MQP, Chalmers AC, Vuong QV, Bowyer MC, Scarlett CJ, 'Characterising the Physical, Phytochemical and Antioxidant Properties of the Tuckeroo (Cupaniopsis anacardioides) Fruit', TECHNOLOGIES, 5 (2017) [C1]
DOI 10.3390/technologies5030057
Co-authors Michael Bowyer, Vanquan Vuong, Anita Chalmers
2017 Dang TT, Vuong QV, Schreider MJ, Bowyer MC, Altena IAV, Scarlett CJ, 'The Effects of Drying on Physico-Chemical Properties and Antioxidant Capacity of the Brown Alga (Hormosira banksii (Turner) Decaisne)', Journal of Food Processing and Preservation, 41 (2017)

© 2016 Wiley Periodicals, Inc. Hormosira banksii is a rich source of polyphenols, which can be utilized in the food or pharmaceutical industries. This study aimed to determine th... [more]

© 2016 Wiley Periodicals, Inc. Hormosira banksii is a rich source of polyphenols, which can be utilized in the food or pharmaceutical industries. This study aimed to determine the impact of six drying methods on properties of the alga H. banksii. Our data revealed that drying conditions significantly affected recovery yield, residual moisture, extraction yield, total phenolic content (TPC) as well as antioxidant capacity of H. banksii (P < 0.05). Optimal conditions for oven, vacuum and de-humidification were 40, 50 and 50C, respectively, and microwave power is 840 W. Under optimal conditions, H. banksii prepared by freeze, de-humidification and vacuum had significantly higher levels of TPC, total flavonoid content (TFC) and proanthocyanidins as well as possessing stronger antioxidant capacity in comparison with those prepared by sun, microwave and oven drying methods. As freeze drying is costly and time-consuming, de-humidification (50C, air in and out of 11.1 and 15.4%) and vacuum (50C, 10 psi) were recommended for drying H. banksii. Practical Applications: Algae possess various antioxidants with potential benefits for health. Drying is considered as a method for preserving materials, transport with low costs and especially first step for extraction, isolation and purification of active compounds. Thus, it is important to investigate the effects of drying conditions on the properties of the dried alga H. banksii. From the findings, the different drying conditions significantly affected the phytochemical profile and antioxidant activity of the dried alga and the optimal drying conditions could be applied for preparation of dried H. banksii for fu rther processing (extraction, fractionation and isolation of bioactive compounds) as well as potential industrial applications (as a reference for drying H. banksii and other algae).

DOI 10.1111/jfpp.13025
Co-authors Ian Vanaltena, Vanquan Vuong, Michael Bowyer, Maria Schreider
2017 Vu HT, Scarlett CJ, Vuong QV, 'Effects of drying conditions on physicochemical and antioxidant properties of banana (Musa cavendish) peels', DRYING TECHNOLOGY, 35 1141-1151 (2017) [C1]
DOI 10.1080/07373937.2016.1233884
Co-authors Vanquan Vuong
2017 Bhuyan DJ, Sakoff J, Bond DR, Predebon M, Vuong QV, Chalmers AC, et al., 'In vitro anticancer properties of selected Eucalyptus species', IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL, 53 604-615 (2017) [C1]
DOI 10.1007/s11626-017-0149-y
Co-authors Danielle Bond, Vanquan Vuong, Anita Chalmers, Michael Bowyer, Jennette Sakoff, Ian Vanaltena
2017 Pham HNT, van Vuong Q, Bowyer MC, Scarlett CJ, 'Phytochemical profiles and antioxidant capacity of the crude extracts, aqueous- and saponin-enriched butanol fractions of Helicteres hirsuta Lour. leaves and stems', Chemical Papers, 1-10 (2017)

© 2017 Institute of Chemistry, Slovak Academy of Sciences This study aimed to compare phytochemical profiles and antioxidant capacity of various extracts including crude extracts... [more]

© 2017 Institute of Chemistry, Slovak Academy of Sciences This study aimed to compare phytochemical profiles and antioxidant capacity of various extracts including crude extracts, aqueous- and saponin-enriched butanol fractions prepared from the stems and leaves of Helicteres hirsuta Lour. The results revealed that all the three powdered extracts from the leaves and the stems possessed high levels of phenolics (177.07¿241.03 mg GAE g -1 ), flavonoids (158.03¿280.06 mg CE g -1 ) and saponins (165.77¿1035.33 mg ESE g -1 ) and exhibited strong antioxidant capacity. HPLC analysis identified nine major compounds in the leaf powder crude extract; however, the leaf aqueous fraction had three extra compounds; whereas, the saponin-enriched butanol leaf fraction had seven extra compounds. For the stems, twelve main compounds were evident in either the powdered crude extract or the aqueous fraction, and five new compounds were revealed in the saponin-enriched butanol fraction. The findings revealed that the powdered aqueous fractions and saponin-enriched butanol fractions are potential sources of biologically active compounds for further investigation and industrial utilisation.

DOI 10.1007/s11696-017-0216-6
Co-authors Michael Bowyer, Vanquan Vuong
2017 Dang TT, van Vuong Q, Schreider MJ, Bowyer MC, van Altena IA, Scarlett CJ, 'Optimisation of ultrasound-assisted extraction conditions for phenolic content and antioxidant activities of the alga Hormosira banksii using response surface methodology', Journal of Applied Phycology, 1-13 (2017)

© 2017 Springer Science+Business Media Dordrecht This study aimed to optimise ultrasound-assisted extraction (UAE) conditions of the brown alga Hormosira banksii for total phenol... [more]

© 2017 Springer Science+Business Media Dordrecht This study aimed to optimise ultrasound-assisted extraction (UAE) conditions of the brown alga Hormosira banksii for total phenolic content (TPC) and antioxidant activities including total antioxidant capacity (ABTS), DPPH free radical scavenging capacity (DPPH) and ferric reducing antioxidant power (FRAP) using response surface methodology (RSM). Box¿Behnken design was employed to assess the effect of ultrasonic temperature, time and power on the TPC and antioxidant activities of the extracts. The results showed that RSM was an accurate and reliable method in predicting TPC and antioxidant activities (ABTS, DPPH and FRAP) of the extracts with R 2 values of 0.97, 0.96, 0.92 and 0.94, respectively. The ultrasonic temperature and time had the significant impact on TPC and antioxidant capacities. The optimal UAE conditions for the maximal values of TPC and antioxidant activities were of 30 °C, 60 min and power 60%, or 150 W. The values of TPC and antioxidant activities (ABTS, DPPH, FRAP) achieved under these parameters were 23.12 (mg GAE g -1 ), 85.64 (mg TE g -1 ), 47.24 (mg TE g -1 ) and 12.56 (mg TE g -1 ), respectively. UAE was found to be more efficient in comparison to conventional extraction, with shorter time for extraction and higher of TPC level and antioxidant activities. Therefore, ultrasonic-assisted extraction using RSM is effective for extraction and further isolation and purification of phenolic compounds from H. banksii. In addition, this alga could be a potential rich source of natural antioxidants applied in food and pharmaceutical fields.

DOI 10.1007/s10811-017-1162-y
Co-authors Michael Bowyer, Vanquan Vuong, Ian Vanaltena
2017 Pham HNT, Vuong QV, Bowyer MC, Scarlett CJ, 'Effect of extraction solvents and thermal drying methods on bioactive compounds and antioxidant properties of Catharanthus roseus (L.) G. Don (Patricia White cultivar)', Journal of Food Processing and Preservation, (2017)

© 2017 Wiley Periodicals, Inc. Catharanthus roseus (L.) G. Don (C. roseus) is well known as an important medicinal plant, with compounds such as the vinca alkaloids isolated for ... [more]

© 2017 Wiley Periodicals, Inc. Catharanthus roseus (L.) G. Don (C. roseus) is well known as an important medicinal plant, with compounds such as the vinca alkaloids isolated for their anticancer activity. As such, it is important to determine the effective solvent for bioactive compound extraction from this plant and the suitable drying methods for preparation of starting material. The aims of this study were to determine the effect of extraction solvents and drying methods on bioactive compounds and antioxidant properties of C. roseus. Water was found to be the optimal solvent for phenolic and flavonoid extraction; whereas, methanol was the best solvent for saponin and proanthocyanidin extraction. The data also revealed that vacuum drying at 50°C was suitable for drying the leaf and the flower which contained high levels of phenolics and flavonoids, while infrared drying at 35°C was recommended for drying the stem and the root which had high saponin content. Practical applications: This study suggested that different parts of C. roseus had different suitable thermal drying methods. For the leaf and the flower, vacuum drying at 50°C was the optimal drying method, whereas infrared drying at 35°C was suitable for drying the stem and the root. These drying conditions can be easily applied for preparation of dried plant parts with high levels of bioactive compounds in the large scale. Importantly, the data indicated that the stem and the root of C. roseus which were considered as waste when the leaf was used for exploiting alkaloids, possessed great content of saponins. Therefore, these parts can be further used for isolation and purification of saponins.

DOI 10.1111/jfpp.13199
Co-authors Vanquan Vuong, Michael Bowyer
2017 Pristijono P, Papoutsis K, Scarlett CJ, Bowyer MC, Vuong QV, Stathopoulos CE, Golding JB, 'Postharvest UV-C treatment combined with 1-methylcyclopropene (1-MCP), followed by storage in continuous low-level ethylene atmosphere, improves the quality of tomatoes', Journal of Horticultural Science and Biotechnology, 92 521-529 (2017)

© 2017 The Journal of Horticultural Science &amp; Biotechnology Trust. Mature green tomatoes (Solanum lycopersicum L. cv Neang Pich) were exposed to 13.6 kJ m -2 UV-C or 0.5¿... [more]

© 2017 The Journal of Horticultural Science & Biotechnology Trust. Mature green tomatoes (Solanum lycopersicum L. cv Neang Pich) were exposed to 13.6 kJ m -2 UV-C or 0.5¿µL L -1 1-MCP or combination of 13.6 kJ m -2 UV-C and 0.5¿µL L -1 1-MCP, with appropriate untreated controls. After treatment, tomatoes were stored in air containing 0.1¿µL L -1 ethylene at 20°C and 100% RH. The untreated fruit ripened significantly faster than those of all other treatments. UV-C treatment alone was able to delay fruit ripening by up to 5 days longer compared to untreated fruits whilst the additional of 1-MCP further delayed fruit ripening. UV-C and 1-MCP treatments alone or in combination had significantly slower ethylene production rates throughout the storage period. The fruit treated with the combination of 1-MCP and UV-C was significantly firmer and had higher total phenolic content compared to that of the other treatments. However, there was no difference between treatments in soluble solids content/titratable acids ratio, chlorophyll content, lycopene content and total antioxidant activity. These results show that UV-C and 1-MCP treatment delay ripening and improve the quality of tomatoes in the pr esence of low-level ethylene during storage. This new treatment could be used to extend the shelf-life of mature green tomatoes through the supply chain without the use of refrigeration.

DOI 10.1080/14620316.2017.1300512
Co-authors Vanquan Vuong, Michael Bowyer
2017 Scarpa A, Chang DK, Nones K, Corbo V, Patch A-M, Bailey P, et al., 'Whole-genome landscape of pancreatic neuroendocrine tumours', NATURE, 543 65-+ (2017) [C1]
DOI 10.1038/nature21063
Citations Scopus - 5Web of Science - 8
2017 Pristijono P, Papoutsis K, Scarlett CJ, Bowyer MC, Vuong QV, Stathopoulos CE, Golding JB, 'Postharvest UV-C treatment combined with 1-methylcyclopropene (1-MCP), followed by storage in continuous low-level ethylene atmosphere, improves the quality of tomatoes', The Journal of Horticultural Science and Biotechnology, 92 521-529 (2017) [C1]
DOI 10.1080/14620316.2017.1300512
Co-authors Michael Bowyer
2017 Bowyer MC, Pristijono P, Scarlett C, Vuong Q, Stathopoulos C, Jessup A, Golding J, 'Use of low-pressure storage to improve the quality of tomatoes', The journal of horticultural science & biotechnology, (2017)
DOI 10.1080/14620316.2017.1301222
Co-authors Vanquan Vuong, Michael Bowyer
2017 Saberi B, Chockchaisawasdee S, Golding JB, Scarlett CJ, Stathopoulos CE, 'Physical and mechanical properties of a new edible film made of pea starch and guar gum as affected by glycols, sugars and polyols', International Journal of Biological Macromolecules, 104 345-359 (2017) [C1]
DOI 10.1016/j.ijbiomac.2017.06.051
2017 Nguyen VT, Bowyer MC, Van Altena IA, Scarlett CJ, 'Microwave-assisted extraction as an advanced technique for optimization of saponin yield and antioxidant potential from Phyllanthus amarus', Separation Science and Technology (Philadelphia), 1-11 (2017)

© 2017 Taylor &amp; Francis The objective of this study was to apply microwave-assisted extraction (MAE) as an advanced technique for optimization of saponin yield and antioxid... [more]

© 2017 Taylor & Francis The objective of this study was to apply microwave-assisted extraction (MAE) as an advanced technique for optimization of saponin yield and antioxidant potential from Phyllanthus amarus. The findings indicated that the optimal MAE parameters consisted of 100% methanol, irradiation time 4 s/min, extraction time 50 min, and solvent to sample ratio 50 mL/g. Under these optimal parameters, saponin content (SC), saponin extraction efficiency (SEE), and total phenolic content (TPC) of P. amarus were 229.5 mg escin equivalents (EEs)/g dried sample, 82.8%, and 40.7 mg gallic acid equivalents (GAEs)/g dried sample, respectively. The antioxidant capacity of P. amarus in terms of 2,2´-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) radical scavenging capacity (ARSC), 2,2-diphenyl-1-picryl-hydrazil radical scavenging capacity (DRSC), and ferric reducing antioxidant power (FRAP) were 487.3, 330.6, and 233.5 mg trolox equivalents (TEs)/g dried sample, respectively. These measured values were not significantly different from the predicted values by response surface methodology (227.9 mg EE/g dried sample, 82.1%, and 39.2 mg GAE/g dried sample for SC, SEE, and TPC and 484.8, 297.3, and 226.6 mg TE/g dried sample for ARSC, DRSC, and FRAP, respectively). Hence, the optimal MAE parameters are suggested for effective extraction of saponins from P. amarus for further investigations and applications.

DOI 10.1080/01496395.2017.1374972
Co-authors Michael Bowyer, Ian Vanaltena
2017 Humphris JL, Patch A-M, Nones K, Bailey PJ, Johns AL, Mckay S, et al., 'Hypermutation In Pancreatic Cancer', GASTROENTEROLOGY, 152 68-+ (2017) [C1]
DOI 10.1053/j.gastro.2016.09.060
Citations Scopus - 2Web of Science - 4
2017 Saberi B, Chockchaisawasdee S, Golding JB, Scarlett CJ, Stathopoulos CE, 'Development of biocomposite films incorporated with different amounts of shellac, emulsifier, and surfactant', Food Hydrocolloids, 72 174-184 (2017) [C1]
DOI 10.1016/j.foodhyd.2017.05.042
2017 Wong M, Tee AEL, Milazzo G, Bell JL, Poulos RC, Atmadibrata B, et al., 'The Histone Methyltransferase DOT1L Promotes Neuroblastoma by Regulating Gene Transcription.', Cancer Res, 77 2522-2533 (2017)
DOI 10.1158/0008-5472.CAN-16-1663
Co-authors Xu Zhang
2017 Bhuyan DJ, Vuong QV, Bond DR, Chalmers AC, van Altena IA, Bowyer MC, Scarlett CJ, 'Exploring the Least Studied Australian Eucalypt Genera: Corymbia and Angophora for Phytochemicals with Anticancer Activity against Pancreatic Malignancies', CHEMISTRY & BIODIVERSITY, 14 (2017) [C1]
DOI 10.1002/cbdv.201600291
Citations Scopus - 1Web of Science - 1
Co-authors Ian Vanaltena, Michael Bowyer, Danielle Bond, Anita Chalmers, Vanquan Vuong
2017 Papoutsis K, Pristijono P, Golding JB, Stathopoulos CE, Bowyer MC, Scarlett CJ, Vuong QV, 'Enhancement of the total phenolic compounds and antioxidant activity of aqueous Citrus limon L. pomace extract using microwave pretreatment on the dry powder', Journal of Food Processing and Preservation, 41 (2017)

© 2016 Wiley Periodicals, Inc. The effect of microwave pretreatment on the levels of total phenolic compounds, flavonoids, proanthocyanidins, and individual major compounds as we... [more]

© 2016 Wiley Periodicals, Inc. The effect of microwave pretreatment on the levels of total phenolic compounds, flavonoids, proanthocyanidins, and individual major compounds as well as the total antioxidant activity of the dried lemon pomace was investigated. The results showed that microwave pretreatment significantly affected all the examined parameters. The total phenolic content, total flavonoids, proanthocyanidins, as well as the total antioxidant activity significantly increased as the microwave radiation time and power increased (e.g., 2.5-fold for phenolics, 1.4-fold for flavonoids, and 5.5-fold for proanthocyanidins); however, irradiation of more than 480¿W for 5¿min resulted in the decrease of these parameters. These findings indicate that microwave irradiation time and power may enhance higher levels of the phenolic compounds as well as the antioxidant capacity of the dried lemon pomace powder. However, higher and longer irradiation may lead to a degradation of phenolic compounds and lower the antioxidant capacity of the dried lemon pomace. Practical applications: Lemon pomace could be a good source of bioactive compounds and antioxidants. Microwave irradiation could be applied for the enhancement of the total phenolic compounds and antioxidants of the lemon pomace-dried powder. The findings of this study can be applied for enhancing the bioactive compounds and the antioxidant activity of the dried lemon pomace for further extraction, isolation, and utilisation.

DOI 10.1111/jfpp.13152
Co-authors Michael Bowyer, Vanquan Vuong
2017 Saberi B, Chockchaisawasdee S, Golding JB, Scarlett CJ, Stathopoulos CE, 'Characterization of pea starch-guar gum biocomposite edible films enriched by natural antimicrobial agents for active food packaging', Food and Bioproducts Processing, 105 51-63 (2017) [C1]

© 2017 Antimicrobial activity of epigallocatechin-3-gallate (EGCG) and two native Australian plants blueberry ash (BBA) fruit and macadamia (MAC) skin extracts against nine patho... [more]

© 2017 Antimicrobial activity of epigallocatechin-3-gallate (EGCG) and two native Australian plants blueberry ash (BBA) fruit and macadamia (MAC) skin extracts against nine pathogenic and spoilage bacteria and seven strains of fungi, using an agar well diffusion assay were investigated. The minimum inhibitory concentrations (MIC) of these compounds were calculated using 96-well microtiter plates method. Finally, active antimicrobial packaging films were prepared by incorporation of EGCG, BBA and MAC extracts at 1-, 2-, 3-, and 4-fold of their correspondence MIC values into edible films based on pea starch and guar gum (PSGG). The antimicrobial activity of films was investigated against target microorganisms by agar disc diffusion technique and quantified using the viable cell count assay. Among the test microorganisms, Salmonella typhimurium and Rhizopus sp. were the most resistance to active films. Films containing EGCG showed the highest activity against all test strains. As the concentration of compounds increased higher than 2¿×¿MIC, the mechanical characteristics of the films were affected considerably. The results indicated that EGCG-PSGG, BBA-PSGG and MAC-PSGG films can be used as active food packaging systems for preserving food safety and prolonging the shelf-life of the packaged food.

DOI 10.1016/j.fbp.2017.06.003
2017 Pristijono P, Bowyer MC, Scarlett CJ, Vuong QV, Stathopoulos CE, Golding JB, 'The Effect of Postharvest UV-C Treatment and Associated with Different Storage Conditions on the Quality of Tahitian Limes (Citrus latifolia)', Journal of Food and Nutritional Disorders, (2017)
DOI 10.4172/2324-9323.1000230
Co-authors Michael Bowyer, Vanquan Vuong
2017 Nguyen VT, Sakoff JA, Scarlett CJ, 'Physicochemical, Antioxidant, and Cytotoxic Properties of Xao Tam Phan (Paramignya trimera) Root Extract and Its Fractions.', Chem Biodivers, 14 (2017)
DOI 10.1002/cbdv.201600396
Co-authors Jennette Sakoff
2017 Thakur R, Saberi B, Pristijono P, Stathopoulos C, Golding J, Scarlett C, et al., 'Use of response surface methodology (RSM) to optimize pea starch¿chitosan novel edible film formulation', Journal of Food Science and Technology, (2017)
DOI 10.1007/s13197-017-2664-y
Co-authors Vanquan Vuong, Michael Bowyer
2017 Nguyen VT, Sakoff JA, Scarlett CJ, 'Physicochemical Properties, Antioxidant and Cytotoxic Activities of Crude Extracts and Fractions from Phyllanthus amarus.', Medicines (Basel), 4 (2017)
DOI 10.3390/medicines4020042
2017 Pham HNT, Vuong QV, Bowyer MC, Scarlett CJ, 'Optimization of ultrasound-assisted extraction of Helicteres hirsuta Lour. for enhanced total phenolic compound and antioxidant yield', Journal of Applied Research on Medicinal and Aromatic Plants, (2017)

© 2017 Elsevier GmbH. Using response surface methodology, this study aimed to optimize five different ultrasound extraction parameters; including ultrasonic temperature, ultrason... [more]

© 2017 Elsevier GmbH. Using response surface methodology, this study aimed to optimize five different ultrasound extraction parameters; including ultrasonic temperature, ultrasonic time, ultrasonic power, sample-to-solvent ratio and methanol concentration, for maximal extraction of bioactive compounds and antioxidant capacity from Helicteres hirsuta Lour. (H. hirsuta), which has been used as a traditional medicine for malaria and diabetes treatment. Results showed that either ultrasonic temperature or sample-to-solvent ratio had a greater influence on extraction efficiency of total phenolics, flavonoids and antioxidant capacity than that of methanol concentration. Optimal extraction conditions were determined to be ultrasonic temperature of 60. °C, ultrasonic time of 25. min, ultrasonic power of 150W, sample-to-solvent ratio of 3:100. g/mL, and a solvent composition of 40% (v/v) aqueous methanol. Under these conditions, 15.97. mg GAE/g of phenolics, 16.42. mg CE/g of flavonoids, and 13.34. g/100. g of extractable solids were obtained. The highest values for DPPH, ABTS and FRAP assays were also obtained under these conditions, with the exception of CUPRAC (88% maximum value). These optimal extraction conditions can be applied to prepare powdered crude extract for further isolation and purification of individual bioactive compounds for potential use in the pharmaceutical industry.

DOI 10.1016/j.jarmap.2017.07.002
Co-authors Vanquan Vuong, Michael Bowyer
2017 Thanh VN, Scarlett CJ, Bowyer MC, Phuong DN, Quan VV, 'Impact of Different Extraction Solvents on Bioactive Compounds and Antioxidant Capacity from the Root of Salacia chinensis L.', JOURNAL OF FOOD QUALITY, (2017) [C1]
DOI 10.1155/2017/9305047
Co-authors Michael Bowyer, Vanquan Vuong
2017 Papoutsis K, Pristijono P, Golding JB, Stathopoulos CE, Bowyer MC, Scarlett CJ, Vuong QV, 'Effect of vacuum-drying, hot air-drying and freeze-drying on polyphenols and antioxidant capacity of lemon (Citrus limon) pomace aqueous extracts', INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY, 52 880-887 (2017) [C1]
DOI 10.1111/ijfs.13351
Citations Scopus - 1
Co-authors Michael Bowyer, Vanquan Vuong
2017 Bhuyan DJ, Vuong QV, Chalmers AC, Bowyer MC, van Altena IA, Scarlett CJ, 'Phytochemical, antibacterial and antifungal properties of an aqueous extract of Eucalyptus microcorys leaves', SOUTH AFRICAN JOURNAL OF BOTANY, 112 180-185 (2017) [C1]
DOI 10.1016/j.sajb.2017.05.030
Co-authors Vanquan Vuong, Ian Vanaltena, Anita Chalmers, Michael Bowyer
2017 Van TN, Sakoff JA, Scarlett CJ, 'Physicochemical Properties, Antioxidant and Anti-proliferative Capacities of Dried Leaf and Its Extract from Xao tam phan (Paramignya trimera)', CHEMISTRY & BIODIVERSITY, 14 (2017) [C1]
DOI 10.1002/cbdv.201600498
Citations Scopus - 1
Co-authors Jennette Sakoff
2017 Hong NTP, Quan VV, Bowyer MC, Scarlett CJ, 'Optimum conventional extraction conditions for phenolics, flavonoids, and antioxidant capacity of Helicteres hirsuta Lour.', ASIA-PACIFIC JOURNAL OF CHEMICAL ENGINEERING, 12 332-347 (2017) [C1]
DOI 10.1002/apj.2076
Co-authors Michael Bowyer, Vanquan Vuong
2016 Saberi B, Thakur R, Vuong QV, Chockchaisawasdee S, Golding JB, Scarlett CJ, Stathopoulos CE, 'Optimization of physical and optical properties of biodegradable edible films based on pea starch and guar gum', Industrial Crops and Products, 86 342-352 (2016) [C1]

© 2016 Elsevier B.V. The influence of process variables (pea starch, guar gum and glycerol) on the viscosity (V), solubility (SOL), moisture content (MC), transparency (TR), Hunt... [more]

© 2016 Elsevier B.V. The influence of process variables (pea starch, guar gum and glycerol) on the viscosity (V), solubility (SOL), moisture content (MC), transparency (TR), Hunter parameters (L, a, and b), total color difference (dE), yellowness index (YI), and whiteness index (WI) of the pea starch based edible films was studied using three factors with three level Box-Behnken response surface design. The individual linear effect of pea starch, guar and glycerol was significant (p < 0.05) on all the responses. However, a value was only significantly (p < 0.05) affected by pea starch and guar gum in a positive and negative linear term, respectively. The effect of interaction of starch × glycerol was also significant (p < 0.05) on TR of edible films. Interaction between independent variables starch × guar gum had a significant impact on the b and YI values. The quadratic regression coefficient of pea starch showed a significant effect (p < 0.05) on V, MC, L, b, dE, YI, and WI; glycerol level on dE and WI; and guar gum on dE and SOL value. The results were analyzed by Pareto analysis of variance (ANOVA) and the second order polynomial models were developed from the experimental design with reliable and satisfactory fit with the corresponding experimental data and high coefficient of determination (R 2 ) values ( > 0.93). Three-dimensional response surface plots were established to investigate the relationship between process variables and the responses. The optimized conditions with the goal of maximizing TR and minimizing SOL, YI and MC were 2.5 g pea starch, 25% glycerol and 0.3 g guar gum. Results revealed that pea starch/guar gum edible films with appropriate physical and optical characteristics can be effectively produced and successfully applied in the food packaging industry.

DOI 10.1016/j.indcrop.2016.04.015
Citations Scopus - 11Web of Science - 7
Co-authors Vanquan Vuong
2016 Bailey P, Chang DK, Nones K, Johns AL, Patch AM, Gingras MC, et al., 'Genomic analyses identify molecular subtypes of pancreatic cancer', Nature, 531 47-52 (2016) [C1]

© 2016 Macmillan Publishers Limited. All rights reserved. Integrated genomic analysis of 456 pancreatic ductal adenocarcinomas identified 32 recurrently mutated genes that aggreg... [more]

© 2016 Macmillan Publishers Limited. All rights reserved. Integrated genomic analysis of 456 pancreatic ductal adenocarcinomas identified 32 recurrently mutated genes that aggregate into 10 pathways: KRAS, TGF-ß, WNT, NOTCH, ROBO/SLIT signalling, G1/S transition, SWI-SNF, chromatin modification, DNA repair and RNA processing. Expression analysis defined 4 subtypes: (1) squamous; (2) pancreatic progenitor; (3) immunogenic; and (4) aberrantly differentiated endocrine exocrine (ADEX) that correlate with histopathological characteristics. Squamous tumours are enriched for TP53 and KDM6A mutations, upregulation of the TP63¿N transcriptional network, hypermethylation of pancreatic endodermal cell-fate determining genes and have a poor prognosis. Pancreatic progenitor tumours preferentially express genes involved in early pancreatic development (FOXA2/3, PDX1 and MNX1). ADEX tumours displayed upregulation of genes that regulate networks involved in KRAS activation, exocrine (NR5A2 and RBPJL), and endocrine differentiation (NEUROD1 and NKX2-2). Immunogenic tumours contained upregulated immune networks including pathways involved in acquired immune suppression. These data infer differences in the molecular evolution of pancreatic cancer subtypes and identify opportunities for therapeutic development.

DOI 10.1038/nature16965
Citations Scopus - 221Web of Science - 222
2016 Nguyen VT, Pham NMQ, Vuong QV, Bowyer MC, van Altena IA, Scarlett CJ, 'Phytochemical retention and antioxidant capacity of xao tam phan (Paramignya trimera) root as prepared by different drying methods', Drying Technology, 34 324-334 (2016) [C1]

© 2016, Taylor &amp; Francis Group, LLC. Xao tam phan (Paramignya trimera (Oliv.) Guillaum) has been used as an herbal medicine for the treatment of cancer or cancer-like disea... [more]

© 2016, Taylor & Francis Group, LLC. Xao tam phan (Paramignya trimera (Oliv.) Guillaum) has been used as an herbal medicine for the treatment of cancer or cancer-like diseases in recent years, particularly in Vietnam. Drying is an important step for preparation of dried materials for storage and further investigation; however, the effects of drying must be taken into account when processing samples, because this can have profound effects on the stability of phytochemical compounds and the biological activity of the dried P. trimera root. As such, this study assessed the effects of four different drying methods (conventional, hot air, vacuum, and microwave) on phytochemical retention and antioxidant capacity of P. trimera root, to identify an optimal drying method for P. trimera root. The results showed that the drying methods significantly affected phytochemical levels and antioxidant capacity of P. trimera root and that among the four drying methods tested, microwave drying (400¿W) had the highest levels of phytochemical compounds, with total phenolic, total flavonoid, proanthocyanidin, and saponin contents of 11.27¿mg GAE, 19.88¿mg RE, 3.98¿mg CE, and 267.15¿mg EE/gram of dried sample, respectively. Dried sample prepared using this method had antioxidant capacity comparable to that of other drying methods. In addition, this method had the shortest drying time (0.28¿h) and consumed the least energy (0.28¿kWh). Therefore, microwave drying should be considered for drying P. trimera root for further investigation and utilization.

DOI 10.1080/07373937.2015.1053566
Citations Scopus - 6Web of Science - 8
Co-authors Ian Vanaltena, Michael Bowyer, Vanquan Vuong
2016 Nguyen VT, Bowyer MC, Van Altena IA, Scarlett CJ, 'Optimisation of microwave-assisted extraction from Phyllanthus amarus for phenolic compounds-enriched extracts and antioxidant capacity', CHEMICAL PAPERS, 70 713-725 (2016) [C1]
DOI 10.1515/chempap-2016-0009
Citations Web of Science - 1
Co-authors Ian Vanaltena, Michael Bowyer
2016 Gingras MC, Covington KR, Chang DK, Donehower LA, Gill AJ, Ittmann MM, et al., 'Ampullary Cancers Harbor ELF3 Tumor Suppressor Gene Mutations and Exhibit Frequent WNT Dysregulation', Cell Reports, 14 907-919 (2016) [C1]

© 2016 The Authors. The ampulla of Vater is a complex cellular environment from which adenocarcinomas arise to form a group of histopathologically heterogenous tumors. To evaluat... [more]

© 2016 The Authors. The ampulla of Vater is a complex cellular environment from which adenocarcinomas arise to form a group of histopathologically heterogenous tumors. To evaluate the molecular features of these tumors, 98 ampullary adenocarcinomas were evaluated and compared to 44 distal bile duct and 18 duodenal adenocarcinomas. Genomic analyses revealed mutations in the WNT signaling pathway among half of the patients and in all three adenocarcinomas irrespective of their origin and histological morphology. These tumors were characterized by a high frequency of inactivating mutations of ELF3, a high rate of microsatellite instability, and common focal deletions and amplifications, suggesting common attributes in the molecular pathogenesis are at play in these tumors. The high frequency of WNT pathway activating mutation, coupled with small-molecule inhibitors of ß-catenin in clinical trials, suggests future treatment decisions for these patients may be guided by genomic analysis.

DOI 10.1016/j.celrep.2015.12.005
Citations Scopus - 6
2016 Weidenhofer JC, Colvin EK, Bond DR, Scarlett CJ, 'Animal models of pancreatic cancer and their application in clinical research', Gastrointestinal Cancer : Targets and Therapy, 2016 31-39 (2016) [C1]
Co-authors Judith Weidenhofer, Danielle Bond
2016 Thuy Pham HN, Nguyen VT, Vuong QV, Bowyer MC, Scarlett CJ, 'Bioactive compound yield and antioxidant capacity of Helicteres hirsuta Lour. stem as affected by various solvents and drying methods', Journal of Food Processing and Preservation, (2016)
DOI 10.1111/jfpp.12879
Citations Scopus - 1
Co-authors Michael Bowyer, Vanquan Vuong
2016 Papoutsis K, Pristijono P, Golding J, Stathopoulos C, Bowyer M, Scarlett C, Vuong QV, 'Optimisation of aqueous extraction conditions for the recovery of phenolic compounds and antioxidants from lemon pomace', International Journal of Food Science & Technology, 51 2009-2018 (2016) [C1]
DOI 10.1111/ijfs.13168
Citations Scopus - 2Web of Science - 2
Co-authors Michael Bowyer, Vanquan Vuong
2016 Papoutsis K, Pristijono P, Golding JB, Stathopoulos CE, Scarlett CJ, Bowyer MC, Vuong QV, 'Impact of different solvents on the recovery of bioactive compounds and antioxidant properties from lemon (Citrus limon L.) pomace waste', Food Science and Biotechnology, 25 971-977 (2016) [C1]

© 2016, The Korean Society of Food Science and Technology and Springer Science+Business Media Dordrecht. The effects of different solvents on the recovery of (i) extractable soli... [more]

© 2016, The Korean Society of Food Science and Technology and Springer Science+Business Media Dordrecht. The effects of different solvents on the recovery of (i) extractable solids (ES), (ii) total phenolic compounds (TPC), (iii) total flavonoid content (TFC), (iv) vitamin C, and (v) antioxidant activity from lemon pomace waste were investigated. The results revealed that solvents significantly affected the recovery of ES, TPC, TFC, and antioxidant properties. Absolute methanol and 50% acetone resulted in the highest extraction yields of TPC, whereas absolute methanol resulted in the highest extraction of TFC, and water had the highest recovery of vitamin C. 50% ethanol, and 50% acetone had higher extraction yields for TPC, and TFC, as well as higher antioxidant activity compared with their absolute solvents and water. TPC and TFC were shown to be the major components contributing to the antioxidant activity of lemon pomace.

DOI 10.1007/s10068-016-0158-8
Citations Scopus - 1Web of Science - 1
Co-authors Michael Bowyer, Vanquan Vuong
2016 Saberi B, Vuong QV, Chockchaisawasdee S, Golding JB, Scarlett CJ, Stathopoulos CE, 'Mechanical and Physical Properties of Pea Starch Edible Films in the Presence of Glycerol', JOURNAL OF FOOD PROCESSING AND PRESERVATION, 40 1339-1351 (2016) [C1]
DOI 10.1111/jfpp.12719
Citations Scopus - 3Web of Science - 3
Co-authors Vanquan Vuong
2016 Van TN, Hong NTP, Bowyer MC, van Altena IA, Scarlett CJ, 'Influence of solvents and novel extraction methods on bioactive compounds and antioxidant capacity of Phyllanthus amarus', CHEMICAL PAPERS, 70 556-566 (2016) [C1]
DOI 10.1515/chempap-2015-0240
Citations Scopus - 3Web of Science - 4
Co-authors Michael Bowyer, Ian Vanaltena
2016 Bhuyan D, Vuong QV, Chalmers A, van Altena I, Bowyer M, Scarlett C, 'Investigation of phytochemicals and antioxidant capacity of selected Eucalyptus species using conventional extraction', Chemical Papers, 70 567-575 (2016) [C1]
DOI 10.1515/chempap-2015-0237
Citations Scopus - 4Web of Science - 4
Co-authors Ian Vanaltena, Anita Chalmers, Michael Bowyer, Vanquan Vuong
2016 Papoutsis K, Vuong QV, Pristijono P, Golding JB, Bowyer MC, Scarlett CJ, Stathopoulos CE, 'Enhancing the Total Phenolic Content and Antioxidants of Lemon Pomace Aqueous Extracts by Applying UV-C Irradiation to the Dried Powder', FOODS, 5 (2016) [C1]
DOI 10.3390/foods5030055
Citations Web of Science - 2
Co-authors Michael Bowyer, Vanquan Vuong
2016 Saberi B, Vuong QV, Chockchaisawasdee S, Golding J, Scarlett C, Stathopoulos C, 'Water Sorption Isotherm of Pea Starch Edible Films and Prediction Models', Foods, 5 (2016) [C1]
DOI 10.3390/foods5010001
Citations Web of Science - 3
Co-authors Vanquan Vuong
2016 Chuen TLK, Vuong QV, Hirun S, Bowyer MB, Predebon MJ, Goldsmith CD, et al., 'Antioxidant and anti-proliferative properties of Davidson¿s plum (Davidsonia pruriens F. Muell) phenolic-enriched extracts as affected by different extraction solvents', Journal of Herbal Medicine, 6 187-192 (2016) [C1]
DOI 10.1016/j.hermed.2016.08.005
Citations Scopus - 1Web of Science - 1
Co-authors Jennette Sakoff, Vanquan Vuong, Michael Bowyer
2016 Thakur R, Saberi B, Pristijono P, Golding J, Stathopoulos C, Scarlett C, et al., 'Characterization of rice starch-L-carrageenan biodegradable edible film. Effect of stearic acid on the film properties', INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 93 952-960 (2016) [C1]
DOI 10.1016/j.ijbiomac.2016.09.053
Citations Scopus - 2Web of Science - 1
Co-authors Michael Bowyer, Vanquan Vuong
2016 Nguyen VT, Scarlett CJ, 'Mass Proportion, Bioactive Compounds and Antioxidant Capacity of Carrot Peel as Affected by Various Solvents', TECHNOLOGIES, 4 (2016) [C1]
DOI 10.3390/technologies4040036
2015 Vuong QV, Hirun S, Chuen TLK, Goldsmith CD, Murchie S, Bowyer MC, et al., 'Antioxidant and anticancer capacity of saponin-enriched Carica papaya leaf extracts', International Journal of Food Science and Technology, 50 169-177 (2015) [C1]

© 2014 Institute of Food Science and Technology. Summary: The papaya (Carica papaya) leaf (PL) contains high levels of saponins and polyphenolic compounds, and historically, it h... [more]

© 2014 Institute of Food Science and Technology. Summary: The papaya (Carica papaya) leaf (PL) contains high levels of saponins and polyphenolic compounds, and historically, it has been used as a folk medicine for numerous ailments, including cancer. PL is traditionally prepared by hot water extraction; however, optimised extraction conditions have not been assessed. This study optimised conditions for the extraction of saponins from PL and assessed their antioxidant capacity and antipancreatic cancer activity. Optimisation was achieved using response surface methodology. Saponins and total phenolic compounds were assessed for their antioxidant, free radical scavenging, ion-reducing capacity, and antipancreatic cancer activity. Optimal aqueous extraction conditions were 85 °C, 25 min. and a water-to-leaf ratio of 20:1 mL g -1 . Ethanol extracts demonstrated higher antioxidant, free radical scavenging and ion-reducing capacity, as well as antipancreatic cancer activity. This study revealed that the PL contains numerous bioactive compounds, with significant anticancer activity warranting further studies on the isolation and characterisation of individual bioactive compounds from the PL.

DOI 10.1111/ijfs.12618
Citations Scopus - 12Web of Science - 9
Co-authors Michael Bowyer, Vanquan Vuong
2015 Vuong QV, Van TN, Dang TT, Bhuyan DJ, Goldsmith CD, Sadeqzadeh E, et al., 'Optimization of ultrasound-assisted extraction conditions for euphol from the medicinal plant, Euphorbia tirucalli, using response surface methodology', INDUSTRIAL CROPS AND PRODUCTS, 63 197-202 (2015) [C1]
DOI 10.1016/j.indcrop.2014.09.057
Citations Scopus - 13Web of Science - 11
Co-authors Vanquan Vuong, Michael Bowyer
2015 Nguyen VT, Bowyer MC, Vuong QV, Altena IAV, Scarlett CJ, 'Phytochemicals and antioxidant capacity of Xao tam phan (Paramignya trimera) root as affected by various solvents and extraction methods', Industrial Crops and Products, 67 192-200 (2015) [C1]

© 2015 Elsevier B.V. Xao tam phan (. Paramignya trimera (Oliv.) Guillaum) is a Vietnamese traditionally medicinal plant used in the treatment of numerous cancers. The preparation... [more]

© 2015 Elsevier B.V. Xao tam phan (. Paramignya trimera (Oliv.) Guillaum) is a Vietnamese traditionally medicinal plant used in the treatment of numerous cancers. The preparation of Xao tam phan extracts including solvent type and extraction method have significant effects on extraction efficiency, phytochemical profile and biological activity. This study aimed to investigate the effects of five various solvents (water, acetonitrile, methanol, ethyl acetate and hexane) and three different extraction methods (conventional, ultrasound-assisted and microwave-assisted) on phytochemical yield and antioxidant capacity of P. trimera root from Vietnam. The results indicate that methanol extracted the maximal yield of phytochemicals from P. trimera and exhibited the greatest antioxidant capacity, with eleven compounds were identified and quantified. Microwave-assisted extraction produced the maximal phytochemical yields (except for total flavonoids) and antioxidant capacity, when compared to conventional and ultrasound-assisted extractions. These data reveal that the use of methanol and microwave-assisted extraction are recommended for extraction of biologically active phytochemicals from P. trimera root for application in the nutraceutical and/or pharmaceutical industries.

DOI 10.1016/j.indcrop.2015.01.051
Citations Scopus - 18Web of Science - 19
Co-authors Ian Vanaltena, Vanquan Vuong, Michael Bowyer
2015 Moran-Jones K, Gloss BS, Murali R, Chang DK, Colvin EK, Jones MD, et al., 'Connective tissue growth factor as a novel therapeutic target in high grade serous ovarian cancer', Oncotarget, 6 44551-44562 (2015) [C1]

Ovarian cancer is the most common cause of death among women with gynecologic cancer. We examined molecular profiles of fibroblasts from normal ovary and high-grade serous ovarian... [more]

Ovarian cancer is the most common cause of death among women with gynecologic cancer. We examined molecular profiles of fibroblasts from normal ovary and high-grade serous ovarian tumors to identify novel therapeutic targets involved in tumor progression. We identified 2,300 genes that are significantly differentially expressed in tumor-associated fibroblasts. Fibroblast expression of one of these genes, connective tissue growth factor (CTGF), was confirmed by immunohistochemistry. CTGF protein expression in ovarian tumor fibroblasts significantly correlated with gene expression levels. CTGF is a secreted component of the tumor microenvironment and is being pursued as a therapeutic target in pancreatic cancer. We examined its effect in in vitro and ex vivo ovarian cancer models, and examined associations between CTGF expression and clinico-pathologic characteristics in patients. CTGF promotes migration and peritoneal adhesion of ovarian cancer cells. These effects are abrogated by FG-3019, a human monoclonal antibody against CTGF, currently under clinical investigation as a therapeutic agent. Immunohistochemical analyses of high-grade serous ovarian tumors reveal that the highest level of tumor stromal CTGF expression was correlated with the poorest prognosis. Our findings identify CTGF as a promoter of peritoneal adhesion, likely to mediate metastasis, and a potential therapeutic target in high-grade serous ovarian cancer. These results warrant further studies into the therapeutic efficacy of FG-3019 in high-grade serous ovarian cancer.

DOI 10.18632/oncotarget.6082
Citations Scopus - 7Web of Science - 5
2015 Vuong QV, Hirun S, Chuen TLK, Goldsmith CD, Munro B, Bowyer MC, et al., 'Physicochemical, antioxidant and anti-cancer activity of a Eucalyptus robusta (Sm.) leaf aqueous extract', INDUSTRIAL CROPS AND PRODUCTS, 64 167-174 (2015) [C1]
DOI 10.1016/j.indcrop.2014.10.061
Citations Scopus - 9Web of Science - 8
Co-authors Jennette Sakoff, Anita Chalmers, Vanquan Vuong, Michael Bowyer
2015 Susanto JM, Colvin EK, Pinese M, Chang DK, Pajic M, Mawson A, et al., 'The epigenetic agents suberoylanilide hydroxamic acid and 5-AZA-2' deoxycytidine decrease cell proliferation, induce cell death and delay the growth of MiaPaCa2 pancreatic cancer cells in vivo', International Journal of Oncology, 46 2223-2230 (2015) [C1]

© 2015, Spandidos Publications. All rights reserved. Despite incremental advances in the diagnosis and treatment for pancreatic cancer (PC), the 5-year survival rate remains &lt... [more]

© 2015, Spandidos Publications. All rights reserved. Despite incremental advances in the diagnosis and treatment for pancreatic cancer (PC), the 5-year survival rate remains < 5%. Novel therapies to increase survival and quality of life for PC patients are desperately needed. Epigenetic therapeutic agents such as histone deacetylase inhibitors (HDACi) and DNA methyltransferase inhibitors (DNMTi) have demonstrated therapeutic benefits in human cancer. We assessed the efficacy of these epigenetic therapeutic agents as potential therapies for PC using in vitro and in vivo models. Treatment with HDACi [suberoylanilide hydroxamic acid (SAHA)] and DNMTi [5-AZA-2' deoxycytidine (5-AZA-dc)] decreased cell proliferation in MiaPaCa2 cells, and SAHA treatment, with or without 5-AZA-dc, resulted in higher cell death and lower DNA synthesis compared to 5-AZA-dc alone and controls (DMSO). Further, combination treatment with SAHA and 5-AZA-dc significantly increased expression of p21 WAF1 , leading to G1 arrest. Treatment with epigenetic agents delayed tumour growth in vivo, but did not decrease growth of established pancreatic tumours. In conclusion, these data demonstrate a potential role for epigenetic modifier drugs for the management of PC, specifically in the chemoprevention of PC, in combination with other chemotherapeutic agents.

DOI 10.3892/ijo.2015.2894
Citations Scopus - 1Web of Science - 1
2015 Goldsmith CD, Vuong QV, Sadeqzadeh E, Stathopoulos CE, Roach PD, Scarlett CJ, 'Phytochemical properties and anti-proliferative activity of Olea Europaea L. leaf extracts against pancreatic cancer cells', Molecules, 20 12992-13004 (2015) [C1]
DOI 10.3390/molecules200712992
Citations Scopus - 9Web of Science - 8
Co-authors Paul Roach, Vanquan Vuong
2015 Hirun S, Choi JH, Ayarungsaritkul J, Pawsaut C, Sutthiwanjampa C, Vuong QV, et al., 'Optimization of far-infrared vacuum drying conditions for Miang leaves (Camellia sinensis var. assamica) using response surface methodology', Food Science and Biotechnology, 24 461-469 (2015) [C1]

© 2015, The Korean Society of Food Science and Technology and Springer Science+Business Media Dordrecht. Far-infrared (FIR) vacuum is an advanced drying technique that has recent... [more]

© 2015, The Korean Society of Food Science and Technology and Springer Science+Business Media Dordrecht. Far-infrared (FIR) vacuum is an advanced drying technique that has recently been applied in food processing. Optimal drying conditions for processing tea from Miang leaves using FIR vacuum drying were investigated. Response surface methodology with a central composite design was used to design, analyze, and predict the optimal time and temperature conditions for FIR vacuum drying, taking into account the physicochemical properties of Miang leaves. When the temperature increased from 50 to 65°C and the time from 60 to 120 min, the amount of epicatechin, epicatechin gallate, epigallocatechin gallate, and total catechins significantly (p < 0.05) increased while the moisture content and water activity significantly (p < 0.05) decreased, compared with controls. The physicochemical properties of dried Miang leaves were significantly (p > 0.05) influenced by time and temperature, compared with controls. Drying conditions of 65°C for 120 min are recommended for optimization of drying.

DOI 10.1007/s10068-015-0061-8
Citations Scopus - 3Web of Science - 2
Co-authors Vanquan Vuong
2015 Munro B, Vuong QV, Chalmers AC, Goldsmith CD, Bowyer MC, Scarlett CJ, 'Phytochemical, Antioxidant and Anti-Cancer Properties of Euphorbia tirucalli Methanolic and Aqueous Extracts.', Antioxidants, 4 647-661 (2015) [C1]
DOI 10.3390/antiox4040647
Citations Web of Science - 6
Co-authors Vanquan Vuong, Michael Bowyer, Anita Chalmers
2015 Bhuyan DJ, Quan VV, Chalmers AC, van Altena IA, Bowyer MC, Scarlett CJ, 'Microwave-assisted extraction of Eucalyptus robusta leaf for the optimal yield of total phenolic compounds', INDUSTRIAL CROPS AND PRODUCTS, 69 290-299 (2015) [C1]
DOI 10.1016/j.indcrop.2015.02.044
Citations Scopus - 23Web of Science - 22
Co-authors Ian Vanaltena, Vanquan Vuong, Michael Bowyer, Anita Chalmers
2015 Chuen TLK, Vuong QV, Hirun S, Bowyer MC, Goldsmith CD, Scarlett CJ, 'Optimum aqueous extraction conditions for preparation of a phenolic-enriched Davidson¿s plum (Davidsonia pruriens F. Muell) extract', International Journal of Food Science and Technology, 50 2475-2482 (2015) [C1]
DOI 10.1111/ijfs.12915
Citations Scopus - 3Web of Science - 3
Co-authors Vanquan Vuong, Michael Bowyer
2015 Waddell N, Pajic M, Patch AM, Chang DK, Kassahn KS, Bailey P, et al., 'Whole genomes redefine the mutational landscape of pancreatic cancer', Nature, 518 495-501 (2015) [C1]

© 2015 Macmillan Publishers Limited. All rights reserved. Pancreatic cancer remains one of the most lethal of malignancies and a major health burden. We performed whole-genome se... [more]

© 2015 Macmillan Publishers Limited. All rights reserved. Pancreatic cancer remains one of the most lethal of malignancies and a major health burden. We performed whole-genome sequencing and copy number variation (CNV) analysis of 100 pancreatic ductal adenocarcinomas (PDACs). Chromosomal rearrangements leading to gene disruption were prevalent, affecting genes known to be important in pancreatic cancer (TP5 3, SMAD4, CDKN2A, ARID1A and ROBO2) and new candidate drivers of pancreatic carcinogenesis (KDM6A and PREX2). Patterns of structural variation (variation in chromosomal structure) classified PDACs into 4 subtypes with potential clinical utility: the subtypes were termed stable, locally rearranged, scattered and unstable. A significant proportion harboured focal amplifications, many of which contained druggable oncogenes (ERBB2, MET, FGFR1, CDK6, PIK3R3 and PIK3CA), but at low individual patient prevalence. Genomic instability co-segregated with inactivation of DNA maintenance genes (BRCA1, BRCA2 or PALB2) and a mutational signature of DNA damage repair deficiency. Of 8 patients who received platinum therapy, 4 of 5 individuals with these measures of defective DNA maintenance responded.

DOI 10.1038/nature14169
Citations Scopus - 423Web of Science - 402
2015 Vuong QV, Zammit N, Munro BR, Murchie S, Bowyer MC, Scarlett CJ, 'Effect of drying conditions on physicochemical and antioxidant properties of vitex agnus-castus leaves', Journal of Food Processing and Preservation, (2015) [C1]

© 2015 Wiley Periodicals, Inc. Vitex agnus-castus (VitexAC) leaves have been used for medicinal purposes for many years. Drying is important to prepare starting materials for fur... [more]

© 2015 Wiley Periodicals, Inc. Vitex agnus-castus (VitexAC) leaves have been used for medicinal purposes for many years. Drying is important to prepare starting materials for further processing, as it is associated with production cost and quality of the materials. Herein, the effects of five different drying conditions on the physical, chemical and antioxidant properties of VitexAC were evaluated. The results showed that 30% of dried leaves with moisture content of less than 7% could be produced from fresh leaves. VitexAC leaves dried by freeze and vacuum drying at 65C had higher levels of bioactive compounds as well as higher antioxidant capacity in comparison with other drying conditions, revealing that these drying conditions are more suitable for drying VitexAC leaves. However, freeze drying is costly and time-consuming; thus, vacuum drying at 65C is suggested for drying the VitexAC leaves as the starting materials for further processing steps. Practical Applications: As drying is an important process to prepare starting materials for further processing steps, it is important to compare different drying methods to identify the most suitable method with low cost and less effect on material quality. The results showed that inappropriate drying conditions resulted in big loss of bioactive compounds and antioxidant capacity. Vacuum drying at 65C was found to be the most suitable method, which can be easily applied for drying Vitex agnus-castus leaves in the industrial scale.

DOI 10.1111/jfpp.12506
Citations Scopus - 4Web of Science - 4
Co-authors Michael Bowyer, Vanquan Vuong
2015 Nguyen VT, Vuong QV, Bowyer MC, Altena IAV, Scarlett CJ, 'Effects of Different Drying Methods on Bioactive Compound Yield and Antioxidant Capacity of Phyllanthus amarus', Drying Technology, 33 1006-1017 (2015) [C1]
DOI 10.1080/07373937.2015.1013197
Citations Scopus - 12Web of Science - 15
Co-authors Michael Bowyer, Vanquan Vuong, Ian Vanaltena
2015 Thuy Pham HN, Nguyen VT, Vuong QV, Bowyer MC, Scarlett CJ, 'Effect of Extraction Solvents and Drying Methods on the Physicochemical and Antioxidant Properties of Helicteres hirsuta Lour. Leaves', Technologies, 3 285-301 (2015) [C1]
DOI 10.3390/technologies3040285
Citations Web of Science - 2
Co-authors Vanquan Vuong, Michael Bowyer
2015 Vuong QV, Chalmers AC, Jyoti Bhuyan D, Bowyer MC, Scarlett CJ, 'Botanical, phytochemical, and anticancer properties of the eucalyptus species', Chemistry and Biodiversity, 12 907-924 (2015) [C1]

Copyright © 2015 Verlag Helvetica Chimica Acta AG, Zürich. The genus Eucalyptus (Myrtaceae) is mainly native to Australia; however, some species are now distributed globally. Eu... [more]

Copyright © 2015 Verlag Helvetica Chimica Acta AG, Zürich. The genus Eucalyptus (Myrtaceae) is mainly native to Australia; however, some species are now distributed globally. Eucalyptus has been used in indigenous Australian medicines for the treatment of a range of aliments including colds, flu, fever, muscular aches, sores, internal pains, and inflammation. Eucalyptus oils containing volatile compounds have been widely used in the pharmaceutical and cosmetics industries for a multitude of purposes. In addition, Eucalyptus extracts containing nonvolatile compounds are also an important source of key bioactive compounds, and several studies have linked Eucalyptus extrac ts with anticancer properties. With the increasing research interest in Eucalyptus and its health properties, this review briefly outlines the botanical features of Eucalyptus, discusses its traditional use as medicine, and comprehensively reviews its phytochemical and anticancer properties and, finally, proposes trends for future studies.

DOI 10.1002/cbdv.201400327
Citations Scopus - 8Web of Science - 8
Co-authors Anita Chalmers, Michael Bowyer, Vanquan Vuong
2014 Shahbazi J, Scarlett CJ, Norris MD, Liu B, Haber M, Tee AE, et al., 'Histone Deacetylase 2 and N-Myc reduce p53 protein phosphorylation at serine 46 by repressing gene transcription of tumor protein 53-induced nuclear protein 1', Oncotarget, 5 4257-4268 (2014) [C1]

Myc oncoproteins and histone deacetylases (HDACs) exert oncogenic effects by modulating gene transcription. Paradoxically, N-Myc induces p53 gene expression. Tumor protein 53-indu... [more]

Myc oncoproteins and histone deacetylases (HDACs) exert oncogenic effects by modulating gene transcription. Paradoxically, N-Myc induces p53 gene expression. Tumor protein 53-induced nuclear protein 1 (TP53INP1) phosphorylates p53 protein at serine 46, leading to enhanced p53 activity, transcriptional activation of p53 target genes and programmed cell death. Here we aimed to identify the mechanism through which N-Myc overexpressing p53 wild-type neuroblastoma cells acquired resistance to apoptosis. TP53INP1 was found to be one of the genes most significantly repressed by HDAC2 and N-Myc according to Affymetrix microarray gene expression datasets. HDAC2 and N-Myc reduced TP53INP1 gene expression by direct binding to the TP53INP1 gene promoter, leading to transcriptional repression of TP53INP1, p53 protein de-phosphorylation at serine 46, neuroblastoma cell proliferation and survival. Moreover, low levels of TP53INP1 expression in human neuroblastoma tissues correlated with high levels of N-Myc expression and poor patient outcome, and the BET bromodomain inhibitors JQ1 and I-BET151 reduced N-Myc expression and reactivated TP53INP1 expression in neuroblastoma cells. These findings identify TP53INP1 repression as an important co-factor for N-Myc oncogenesis, and provide further evidence for the potential application of BET bromodomain inhibitors in the therapy of N-Myc-induced neuroblastoma.

Citations Scopus - 17Web of Science - 17
2014 Vuong QV, Hirun S, Chuen TLK, Goldsmith CD, Bowyer MC, Chalmers AC, et al., 'Physicochemical composition, antioxidant and anti-proliferative capacity of a lilly pilly (Syzygium paniculatum) extract', Journal of Herbal Medicine, 4 134-140 (2014) [C1]

Lilly pilly (LP) fruit (Syzygium paniculatum Gaertn.) is widely grown in eastern Australia and has been used as food by indigenous Australians. However, there is limited informati... [more]

Lilly pilly (LP) fruit (Syzygium paniculatum Gaertn.) is widely grown in eastern Australia and has been used as food by indigenous Australians. However, there is limited information on its bioactivity. This study investigated the physicochemical and antioxidant properties of the crude fruit extract, identified its bioactive compounds and also assessed its potential anti-proliferative effect on pancreatic cancer cells. Our data showed that the LP extract was water-soluble and possessed a total phenolic content of 96 mg of gallic acid equivalents (GAE)/g, flavonoid levels of 52 mg catechin equivalents (CAE)/g, proanthocyanidin levels of 29 mg CAE/g. Several phenolic compounds such as gallic acid, chlorogenic acid, catechin and epicatechin were identified in the LP extract with levels of 0.39, 2.35, 0.47 and 2.9 mg/g, respectively. Results from six different antioxidant assays revealed that the LP extract pocessed potent antioxidant and free radical scavenging capacity. Although antioxidant capacity of the extract was lower than that of vitamin E, vitamin C and BHT, it could be significantly improved if the extract was to be further purified. We also showed that the LP extract (200 µg/mL) significantly reduced the viability of MiaPaCa-2 and ASPC-1 pancreatic cancer cells to levels comparable to that of the chemotherapeutic agent gemcitabine. For this reason lilly pilly should be further investigated for its health promoting and potential anti-cancer benefits, particularly for pancreatic cancer. © 2014 Elsevier GmbH.

DOI 10.1016/j.hermed.2014.04.003
Citations Scopus - 8
Co-authors Michael Bowyer, Anita Chalmers, Vanquan Vuong
2014 Nagrial AM, Chang DK, Nguyen NQ, Johns AL, Chantrill LA, Humphris JL, et al., 'Adjuvant chemotherapy in elderly patients with pancreatic cancer', British Journal of Cancer, 110 313-319 (2014) [C1]

Background:Adjuvant chemotherapy improves survival for patients with resected pancreatic cancer. Elderly patients are under-represented in Phase III clinical trials, and as a cons... [more]

Background:Adjuvant chemotherapy improves survival for patients with resected pancreatic cancer. Elderly patients are under-represented in Phase III clinical trials, and as a consequence the efficacy of adjuvant therapy in older patients with pancreatic cancer is not clear. We aimed to assess the use and efficacy of adjuvant chemotherapy in older patients with pancreatic cancer.Methods:We assessed a community cohort of 439 patients with a diagnosis of pancreatic ductal adenocarcinoma who underwent operative resection in centres associated with the AUSn Pancreatic Cancer Genome Initiative.Results:The median age of the cohort was 67 years. Overall only 47% of all patients received adjuvant therapy. Patients who received adjuvant chemotherapy were predominantly younger, had later stage disease, more lymph node involvement and more evidence of perineural invasion than the group that did not receive adjuvant treatment. Overall, adjuvant chemotherapy was associated with prolonged survival (median 22.1 vs 15.8 months; P < 0.0001). Older patients (aged =70) were less likely to receive adjuvant chemotherapy (51.5% vs 29.8%; P < 0.0001). Older patients had a particularly poor outcome when adjuvant therapy was not delivered (median survival=13.1 months; HR 1.89, 95% CI: 1.27-2.78, P=0.002).Conclusion:Patients aged =70 are less likely to receive adjuvant therapy although it is associated with improved outcome. Increased use of adjuvant therapy in older individuals is encouraged as they constitute a large proportion of patients with pancreatic cancer. © 2014 Cancer Research UK.

DOI 10.1038/bjc.2013.722
Citations Scopus - 17Web of Science - 16
2014 Morran DC, Wu J, Jamieson NB, Mrowinska A, Kalna G, Karim SA, et al., 'Targeting mTOR dependency in pancreatic cancer', Gut, 63 1481-1489 (2014) [C1]

Objective: Pancreatic cancer is a leading cause of cancer-related death in the Western world. Current chemotherapy regimens have modest survival benefit. Thus, novel, effective th... [more]

Objective: Pancreatic cancer is a leading cause of cancer-related death in the Western world. Current chemotherapy regimens have modest survival benefit. Thus, novel, effective therapies are required for treatment of this disease. Design Activating KRAS mutation almost always drives pancreatic tumour initiation, however, deregulation of other potentially druggable pathways promotes tumour progression. PTEN loss leads to acceleration of Kras G12D driven pancreatic ductal adenocarcinoma (PDAC) in mice and these tumours have high levels of mammalian target of rapamycin (mTOR) signalling. To test whether these KRAS PTEN pancreatic tumours show mTOR dependence, we compared response to mTOR inhibition in this model, to the response in another established model of pancreatic cancer, KRAS P53. We also assessed whether there was a subset of pancreatic cancer patients who may respond to mTOR inhibition. Results: We found that tumours in KRAS PTEN mice exhibit a remarkable dependence on mTOR signalling. In these tumours, mTOR inhibition leads to proliferative arrest and even tumour regression. Further, we could measure response using clinically applicable positron emission tomography imaging. Importantly, pancreatic tumours driven by activated KRAS and mutant p53 did not respond to treatment. In human tumours, approximately 20% of cases demonstrated low PTEN expression and a gene expression signature that overlaps with murine KRAS PTEN tumours. Conclusions: KRAS PTEN tumours are uniquely responsive to mTOR inhibition. Targeted anti-mTOR therapies may offer clinical benefit in subsets of human PDAC selected based on genotype, that are dependent on mTOR signalling. Thus, the genetic signatures of human tumours could be used to direct pancreatic cancer treatment in the future.

DOI 10.1136/gutjnl-2013-306202
Citations Scopus - 38Web of Science - 37
2014 Vuong QV, Hirun S, Chuen TLK, Goldsmith CD, Bowyer MC, Chalmers AC, et al., 'Physicochemical composition, antioxidant and anti-proliferative capacity of a lilly pilly (Syzygium paniculatum) extract', Journal of Herbal Medicine, 4 134-140 (2014) [C1]

Lilly pilly (LP) fruit (Syzygium paniculatum Gaertn.) is widely grown in eastern Australia and has been used as food by indigenous Australians. However, there is limited informati... [more]

Lilly pilly (LP) fruit (Syzygium paniculatum Gaertn.) is widely grown in eastern Australia and has been used as food by indigenous Australians. However, there is limited information on its bioactivity. This study investigated the physicochemical and antioxidant properties of the crude fruit extract, identified its bioactive compounds and also assessed its potential anti-proliferative effect on pancreatic cancer cells. Our data showed that the LP extract was water-soluble and possessed a total phenolic content of 96 mg of gallic acid equivalents (GAE)/g, flavonoid levels of 52 mg catechin equivalents (CAE)/g, proanthocyanidin levels of 29 mg CAE/g. Several phenolic compounds such as gallic acid, chlorogenic acid, catechin and epicatechin were identified in the LP extract with levels of 0.39, 2.35, 0.47 and 2.9 mg/g, respectively. Results from six different antioxidant assays revealed that the LP extract pocessed potent antioxidant and free radical scavenging capacity. Although antioxidant capacity of the extract was lower than that of vitamin E, vitamin C and BHT, it could be significantly improved if the extract was to be further purified. We also showed that the LP extract (200 µg/mL) significantly reduced the viability of MiaPaCa-2 and ASPC-1 pancreatic cancer cells to levels comparable to that of the chemotherapeutic agent gemcitabine. For this reason lilly pilly should be further investigated for its health promoting and potential anti-cancer benefits, particularly for pancreatic cancer. © 2014 Elsevier GmbH.

DOI 10.1016/j.hermed.2014.04.003
Citations Scopus - 8Web of Science - 8
Co-authors Vanquan Vuong, Michael Bowyer, Anita Chalmers
2014 Vuong QV, Hirun S, Phillips PA, Chuen TLK, Bowyer MC, Goldsmith CD, Scarlett CJ, 'Fruit-derived phenolic compounds and pancreatic cancer: Perspectives from Australian native fruits', JOURNAL OF ETHNOPHARMACOLOGY, 152 227-242 (2014) [C1]
DOI 10.1016/j.jep.2013.12.023
Citations Scopus - 20Web of Science - 17
Co-authors Michael Bowyer, Vanquan Vuong
2014 Hirun S, Utama-ang N, Vuong QV, Scarlett CJ, 'Investigating the Commercial Microwave Vacuum Drying Conditions on Physicochemical Properties and Radical Scavenging Ability of Thai Green Tea', DRYING TECHNOLOGY, 32 47-54 (2014) [C1]
DOI 10.1080/07373937.2013.811249
Citations Scopus - 6Web of Science - 3
Co-authors Vanquan Vuong
2014 Sun Y, Liu PY, Scarlett CJ, Malyukova A, Liu B, Marshall GM, et al., 'Histone deacetylase 5 blocks neuroblastoma cell differentiation by interacting with N-Myc', Oncogene, 33 2987-2994 (2014) [C1]

The N-Myc oncoprotein induces neuroblastoma, which arises from undifferentiated neuroblasts in the sympathetic nervous system, by modulating gene and protein expression and conseq... [more]

The N-Myc oncoprotein induces neuroblastoma, which arises from undifferentiated neuroblasts in the sympathetic nervous system, by modulating gene and protein expression and consequently causing cell differentiation block and cell proliferation. The class IIa histone deacetylase 5 (HDAC5) represses gene transcription, and blocks myoblast, osteoblast and leukemia cell differentiation. Here we showed that N-Myc upregulated HDAC5 expression in neuroblastoma cells. Conversely, HDAC5 repressed the ubiquitin-protein ligase NEDD4 gene expression, increased Aurora A gene expression and consequently upregulated N-Myc protein expression. Genome-wide gene expression analysis and protein co-immunoprecipitation assays revealed that HDAC5 and N-Myc repressed the expression of a common subset of genes by forming a protein complex, whereas HDAC5 and the class III HDAC SIRT2 independently repressed the expression of another common subset of genes without forming a protein complex. Moreover, HDAC5 blocked differentiation and induced proliferation in neuroblastoma cells. Taken together, our data identify HDAC5 as a novel co-factor in N-Myc oncogenesis, and provide the evidence for the potential application of HDAC5 inhibitors in the therapy of N-Myc-induced neuroblastoma and potentially other c-Myc-induced malignancies.. © 2014 Macmillan Publishers Limited All rights reserved 0950-9232/14.

DOI 10.1038/onc.2013.253
Citations Scopus - 15Web of Science - 14
2014 Colyin EK, Scarlett CJ, 'A historical perspective of pancreatic cancer mouse models', SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY, 27 96-105 (2014) [C1]
DOI 10.1016/j.semcdb.2014.03.025
Citations Scopus - 7
2014 Vuong QV, Sadeqzadeh E, Hirun S, Goldsmith CD, Zammitt N, Bowyer MB, et al., 'Phenolic Compounds, Antioxidant and Anti-Cancer Properties of the Australian Maroon Bush Scaevola spinescens (Goodeniaceae)', Journal of Bioanalysis & Biomedicine, S12 (2014) [C1]
DOI 10.4172/1948-593X.S12-002
Co-authors Vanquan Vuong, Judith Weidenhofer, Rick Thorne, Michael Bowyer, Jennette Sakoff
2014 Goldsmith C, Vuong Q, Stathopoulos C, Roach P, Scarlett C, 'Optimization of the Aqueous Extraction of Phenolic Compounds from Olive Leaves', Antioxidants, 3 700-712 (2014) [C1]
DOI 10.3390/antiox3040700
Co-authors Paul Roach, Vanquan Vuong
2014 Vuong Q, Goldsmith C, Dang T, Nguyen V, Bhuyan D, Sadeqzadeh E, et al., 'Optimisation of Ultrasound-Assisted Extraction Conditions for Phenolic Content and Antioxidant Capacity from Euphorbia tirucalli Using Response Surface Methodology', Antioxidants, 3 604-617 (2014) [C1]
DOI 10.3390/antiox3030604
Co-authors Michael Bowyer, Vanquan Vuong
2013 Scarlett CJ, 'Contribution of bone marrow derived cells to the pancreatic tumor microenvironment', Frontiers in Physiology, 4 (2013) [C1]
DOI 10.3389/fphys.2013.00056
Citations Scopus - 7Web of Science - 8
2013 Chang DK, Jamieson NB, Johns AL, Scarlett CJ, Pajic M, Chou A, et al., 'Histomolecular Phenotypes and Outcome in Adenocarcinoma of the Ampulla of Vater', JOURNAL OF CLINICAL ONCOLOGY, 31 1348-1356 (2013) [C1]
DOI 10.1200/JCO.2012.46.8868
Citations Scopus - 35Web of Science - 33
2013 Liu PY, Xu N, Malyukova A, Scarlett CJ, Sun YT, Zhang XD, et al., 'The histone deacetylase SIRT2 stabilizes Myc oncoproteins', CELL DEATH AND DIFFERENTIATION, 20 503-514 (2013) [C1]
DOI 10.1038/cdd.2012.147
Citations Scopus - 70Web of Science - 60
Co-authors Xu Zhang
2013 Vuong QV, Hirun S, Roach PD, Bowyer MC, Phillips PA, Scarlett CJ, 'Effect of extraction conditions on total phenolic compounds and antioxidant activities of Carica papaya leaf aqueous extracts', JOURNAL OF HERBAL MEDICINE, 3 104-111 (2013) [C1]
DOI 10.1016/j.hermed.2013.04.004
Citations Scopus - 40Web of Science - 41
Co-authors Vanquan Vuong, Paul Roach, Michael Bowyer
2012 Biankin AV, Waddell N, Kassahn KS, Gingras M-C, Muthuswamy LB, Johns AL, et al., 'Pancreatic cancer genomes reveal aberrations in axon guidance pathway genes', Nature, 491 399-405 (2012) [C1]
Citations Scopus - 699Web of Science - 666
2012 Das A, Musgrove EA, Sutherland RL, Biankin AV, Humphris JL, Chang DK, et al., 'The prognostic and predictive value of serum CA19.9 in pancreatic cancer', Annals of Oncology, 23 1713-1722 (2012) [C1]
Citations Scopus - 88Web of Science - 77
2011 Marshall GM, Liu PY, Gherardi S, Scarlett CJ, Bedalov A, Xu N, et al., 'SIRT1 promotes N-Myc oncogenesis through a positive feedback loop involving the effects of MKP3 and ERK on N-Myc protein stability', PLoS Genetics, 7 e1002135-e1002135 (2011) [C1]
DOI 10.1371/journal.pgen.1002135
Citations Scopus - 73Web of Science - 63
2011 Colvin EK, Susanto JM, Kench JG, Ong VN, Mawson A, Pinese M, et al., 'Retinoid signaling in pancreatic cancer, injury and regeneration', PLoS ONE, 6 (2011) [C1]
DOI 10.1371/journal.pone.0029075
Citations Scopus - 6Web of Science - 7
2011 Chong JJH, Chandrakanthan V, Xaymardan M, Asli NS, Li J, Ahmed I, et al., 'Adult cardiac-resident MSC-like stem cells with a proepicardial origin', Cell Stem Cell, 9 527-540 (2011) [C1]
DOI 10.1016/j.stem.2011.10.002
Citations Scopus - 184Web of Science - 171
2011 Scarlett CJ, Colvin EK, Pinese M, Chang DK, Morey AL, Musgrove EA, et al., 'Recruitment and activation of pancreatic stellate cells from the bone marrow in pancreatic cancer: A model of tumor-host interaction', PLoS One, 6 1-8 (2011) [C1]
DOI 10.1371/journal.pone.0026088
Citations Scopus - 24
2011 Kwong RA, Scarlett CJ, Kalish LH, Cole IE, Kench JG, Sum EY, et al., 'LMO4 expression in squamous cell carcinoma of the anterior tongue', Histopathology, 58 477-480 (2011) [C3]
DOI 10.1111/j.1365-2559.2011.03765.x
Citations Scopus - 5
2011 Nguyen NQ, Johns AL, Gill AJ, Ring N, Chang DK, Clarkson A, et al., 'Clinical and immunohistochemical features of 34 solid pseudopapillary tumors of the pancreas', Journal of Gastroenterology and Hepatology, 26 267-274 (2011) [C1]
Citations Scopus - 23Web of Science - 18
2010 Xue A, Scarlett CJ, Chung L, Butturini G, Scarpa A, Gandy R, et al., 'Discovery of serum biomarkers for pancreatic adenocarcinoma using proteomic analysis', British Journal of Cancer, 103 391-400 (2010) [C1]
DOI 10.1038/sj.bjc.6605764
Citations Scopus - 32Web of Science - 33
2010 Marshall GM, Gherardi S, Xu N, Neiron Z, Trahair T, Scarlett CJ, et al., 'Transcriptional upregulation of histone deacetylase 2 promotes Myc-induced oncogenic effects', Oncogene, 29 5957-5968 (2010) [C1]
Citations Scopus - 50Web of Science - 49
2009 Pinese M, Scarlett CJ, Kench JG, Colvin EK, Segara D, Henshall SM, et al., 'Messina: A novel analysis tool to identify biologically relevant molecules in disease', PLoS One, 4 (2009) [C1]
DOI 10.1371/journal.pone.0005337
Citations Scopus - 5Web of Science - 5
2009 Biankin AV, Kench JG, Colvin EK, Segara D, Scarlett CJ, Nguyen NQ, et al., 'Expression of S100A2 Calcium-Binding Protein Predicts Response to Pancreatectomy for Pancreatic Cancer', Gastroenterology, 137 558-568 (2009) [C1]
DOI 10.1053/j.gastro.2009.04.009
Citations Scopus - 42Web of Science - 41
2009 Chang DK, Johns AL, Merrett ND, Gill AJ, Colvin EK, Scarlett CJ, et al., 'Margin Clearance and Outcome in Resected Pancreatic Cancer', Journal of Clinical Oncology, 27 2855-2862 (2009) [C1]
DOI 10.1200/JCO.2008.20.5104
Citations Scopus - 152Web of Science - 130
2008 Murphy NC, Scarlett CJ, Kench JG, Sym EYM, Segara D, Colvin EK, et al., 'Expression of LMO4 and outcome in pancreatic ductal adenocarcinoma', British Journal of Cancer, 98 537-541 (2008) [C1]
DOI 10.1038/sj.bjc.6604177
Citations Scopus - 20
2008 Kuo SCL, Gananadha S, Scarlett CJ, Gill A, Smith RC, 'Sporadic pancreatic polypeptide secreting tumors (PPomas) of the pancreas', World Journal of Surgery, 32 1815-1822 (2008) [C1]
DOI 10.1007/s00268-008-9499-7
Citations Scopus - 15Web of Science - 14
2008 Xue A, Scarlett CJ, Jackson CJ, Allen BJ, Smith RC, 'Prognostic significance of growth factors and the urokinase-type plasminogen activator system in pancreatic ductal adenocarcinoma', Pancreas, 36 160-167 (2008) [C1]
DOI 10.1097/MPA.0b013e31815750f0
Citations Scopus - 35
2007 Smith R, Xue A, Gill A, Scarlett C, Saxby A, Clarkson A, Hugh T, 'High expression of plasminogen activator inhibitor-2 (PAI-2) is a predictor of improved survival in patients with pancreatic adenocarcinoma', WORLD JOURNAL OF SURGERY, 31 493-503 (2007) [C1]
DOI 10.1007/s00268-006-0289-9
Citations Scopus - 27Web of Science - 27
2007 Scarlett CJ, Samra JS, Xue A, Baxter RC, Smith RC, 'Classification of pancreatic cystic lesions using SELDI-TOF mass spectrometry', ANZ Journal of Surgery, 77 648-653 (2007) [C1]

Background: The diagnosis of pancreatic cystic lesions is problematical with difficulties arising in the differentiation between malignant, premalignant or benign lesions. This pr... [more]

Background: The diagnosis of pancreatic cystic lesions is problematical with difficulties arising in the differentiation between malignant, premalignant or benign lesions. This preliminary study aimed to analyse pancreatic cyst fluid, using a proteomic approach, to generate reproducible protein profiles to assist in the classification of malignant and non-carcinoma samples. Methods: Pancreatic cyst fluid samples from patients with pancreatic adenocarcinoma and non-carcinoma cystic lesions were analysed on hydrophobic protein chip arrays by surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS). Results: Differential protein expression profiles were observed between pancreatic adenocarcinoma and non-carcinoma cyst fluid samples using SELDI-TOF MS, with 12 protein peaks differentially expressed between pancreatic adenocarcinoma and non-carcinoma. Additionally, unique patterns were observed between the different subtypes of non-carcinoma samples as well as malignant adenocarcinoma. Conclusions: In this preliminary study we used SELDI-TOF MS to identify protein expression profiles of pancreatic cyst fluid, showing a potential to aid in the differential diagnosis of pancreatic cystic lesions. © 2007 Royal Australasian College of Surgeons.

DOI 10.1111/j.1445-2197.2007.04179.x
Citations Scopus - 12
2007 O'Leary MJ, Xue A, Scarlett CJ, Sevette A, Kee AJ, Smith RC, 'Parenteral versus enteral nutrition: Effect on serum cytokines and the hepatic expression of mRNA of suppressor of cytokine signaling proteins, insulin-like growth factor-1 and the growth hormone receptor in rodent sepsis', CRITICAL CARE, 11 (2007) [C1]
DOI 10.1186/cc5972
Citations Scopus - 7Web of Science - 5
2006 Scarlett CJ, Smith RC, Saxby A, Nielsen A, Samra JS, Wilson SR, Baxter RC, 'Proteomic Classification of Pancreatic Adenocarcinoma Tissue Using Protein Chip Technology', Gastroenterology, 130 1670-1678 (2006) [C1]

Background &amp; Aims: Pancreatic adenocarcinoma is a most devastating cancer that presents late and is rapidly progressive. This study aimed to identify unique, tissue-specific... [more]

Background & Aims: Pancreatic adenocarcinoma is a most devastating cancer that presents late and is rapidly progressive. This study aimed to identify unique, tissue-specific protein biomarkers capable of differentiating pancreatic adenocarcinoma (PC) from adjacent uninvolved pancreatic tissue (AP), benign pancreatic disease (B), and nonmalignant tumor tissue (NM). Methods: Tissue samples representing PC (n = 31), AP (n = 44), and B (n = 19) tissue were analyzed on hydrophobic protein chip arrays by surface-enhanced laser desorption/ionization time-of-flight mass spectrometry. Training models were developed using logistic regression and validated using the 10-fold cross-validation approach. Results: The hydrophobic protein chip array revealed 13 protein peaks differentially expressed between PC and AP (receiver operating characteristic [ROC] area under the curve [AUC] , 0.64-0.85), 8 between PC and B (ROC AUC, 0.67-0.78), and 12 between PC and NM tissue (ROC AUC, 0.63-0.81). Logistic regression and cross-validation identified overlapping panels of peaks to develop a training model that distinguished PC from AP (77.4% sensitivity, 84.1% specificity), B (83.9% sensitivity, 78.9% specificity), and NM tissue (58.1% sensit ivity, 90.5% specificity). The final panels selected correctly classified 80.6% of PC and 88.6% of AP samples (ROC AUC, 0.92), 93.5% of PC and 89.5% of B samples (ROC AUC, 0.99), and 71.0% of PC and 92.1% of NM samples (ROC AUC, 0.91). Conclusions: This study used surface-enhanced laser desorption/ionization time-of-flight mass spectrometry to discover a number of protein panels that can distinguish effectively between pancreatic adenocarcinoma, benign, and adjacent pancreatic tissue. Identification of these proteins will add to our understanding of the biology of pancreatic cancer. Furthermore, these protein panels may have important diagnostic implications. © 2006 American Gastroenterological Association Institute.

DOI 10.1053/j.gastro.2006.02.036
Citations Scopus - 38
2006 Scarlett CJ, Saxby AJ, Nielsen AQ, Bell C, Samra JS, Hugh T, et al., 'Proteomic profiling of cholangiocarcinoma: Diagnostic potential of SELDI-TOF MS in malignant bile duct stricture', Hepatology, 44 658-666 (2006) [C1]

Proteomic techniques promise to improve the diagnosis of cholangiocarcinoma (CC) in both tissue and serum as histological diagnosis and existing serum markers exhibit poor sensiti... [more]

Proteomic techniques promise to improve the diagnosis of cholangiocarcinoma (CC) in both tissue and serum as histological diagnosis and existing serum markers exhibit poor sensitivities. We explored the use of surface-enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS) to identify potential protein biomarkers of CC. Twenty-two resected CC samples were compared with adjacent noninvolved bile duct tissue. Serum from patients with CC (n = 20) was compared with patients with benign disease (n = 20), and healthy volunteers (n = 25). Samples were analyzed on hydrophobic protein chips via SELDI-TOF MS, and classification models were developed using logistic regression and cross-validation analysis. Univariate analysis revealed 14 individual peaks differentially expressed between CC and bile duct tissue, 4 peaks between CC and benign disease, and 12 peaks between CC and sera of healthy volunteers. The 4,462 mass-to-charge serum peak had superior discriminatory ability to carbohydrate antigen 19.9 (CA19.9) and carcinoembryonic antigen (CEA) (P = .004; receiver operating characteristic [ROC] area under the curve [AUC] = 0.76, 0.73, and 0.70, respectively). The training models developed panels of peaks that distinguished CC from bile duct tissue (92.5% sensitivity, 92.3% specificity, ROC AUC = 0.96), CC from benign serum (65.0% sensitivity, 70.0% specificity, ROC AUC = 0.83), and CC from sera of healthy volunteers (75.0% sensitivity, 100% specificity; ROC AUC = 0.92). Serum results were further improved with the inclusion of CA19.9 and CEA (ROC AUC = 0.86 and 0.99 for CC vs benign and healthy volunteer serum, respectively). In conclusion, biomarker panels are capable of distinguishing CC from nonmalignant tissue; serum markers have important diagnostic implications for unknown bile duct stricture. Copyright © 2006 by the American Association for the Study of Liver Diseases.

DOI 10.1002/hep.21294
Citations Scopus - 39
2005 Saxby AJ, Nielsen A, Scarlett CJ, Clarkson A, Morey A, Gill A, Smith RC, 'Assessment of HER-2 status in pancreatic adenocarcinoma: Correlation of immunohistochemistry, quantitative real-time RT-PCR, and FISH with aneuploidy and survival', American Journal of Surgical Pathology, 29 1125-1134 (2005) [C1]

HER-2 is a transmembrane growth factor receptor recognized in overexpression as an independent adverse prognostic factor in several cancers. This study measured HER-2 overexpressi... [more]

HER-2 is a transmembrane growth factor receptor recognized in overexpression as an independent adverse prognostic factor in several cancers. This study measured HER-2 overexpression in pancreatic adenocarcinoma at the genetic, transcriptional, and translational level. Expression was gauged with regard to stage, grade, and survival. Pancreatic adenocarcinoma samples (n = 30) were analyzed with immunohistochemical labeling for HER-2 protein, Quantitative real-time reverse transcriptase polymerase chain reaction (Q-RT-PCR) measurement of HER-2 mRNA and fluorescence in situ hybridization (FISH) analysis of HER-2 gene expression. HER-2 expression in benign pancreatic lesions (n = 10) provided a control. Five (17%) of the pancreatic adenocarcinomas scored maximal 3+ immunohistochemistry (IHC) labeling, seven (23%) had significantly increased expression of HER-2 mRNA, while only one (3%) exhibited low level HER-2 gene amplification. Ten (33%) tumors demonstrated aneuploidy. In general, concordance between methodologies was poor, but the best agreement was seen between FISH aneuploidy status and Q-RT-PCR mRNA overexpression (80% agreement), followed by IHC and Q-RT-PCR (73% agreement). The least agreement was seen between IHC and FISH aneuploidy status (67% agreement). Tumor stage was positively associated with HER-2 mRNA and protein expression, but tumor grade and other patient characteristics did not reach statistical significance. A poor survival outcome was demonstrated with positive HER-2 status in all three measures of overexpression (Kaplan-Meier log-rank score; P < 0.01 [IHC], P = 0.05 [Q-RT-PCR] , P = 0.02 [FISH]). Discordance in expression at the nuclear, cytoplasmic, and cell surface levels highlights the limitations of immunohistochemical evaluation alone and stresses the need for further evaluation of response to anti-HER-2 targeted therapies in tumors displaying overexpression in gene copy, mRNA, and receptor protein. Copyright © 2005 by Lippincott Williams & Wilkins.

DOI 10.1097/01.pas.0000160979.85457.73
Citations Scopus - 43
2005 Nielsen A, Scarlett CJ, Samra JS, Gill A, Li Y, Allen BJ, Smith RC, 'Significant overexpression of urokinase-type plasminogen activator in pancreatic adenocarcinoma using real-time quantitative reverse transcription polymerase chain reaction', Journal of Gastroenterology and Hepatology (Australia), 20 256-263 (2005) [C1]

Background and Aims: Overexpression of urokinase-type plasminogen activator (uPA) has been shown to be strongly associated with an increased metastatic potential and poor prognosi... [more]

Background and Aims: Overexpression of urokinase-type plasminogen activator (uPA) has been shown to be strongly associated with an increased metastatic potential and poor prognosis in a variety of human malignancies. It was hypothesized that uPA would be overexpressed in highly metastatic pancreatic cancer. The aims of this study were to analyze uPA mRNA expression in pancreatic cancer and to correlate this to the expression of uPA protein and to the stage of the disease. Methods: Twenty-one pancreatic adenocarcinoma, six ampullary carcinoma and 10 benign mucinous cystadenoma samples, all with adjacent normal tissue, were collected. uPA mRNA was measured using real-time quantitative reverse transcription polymerase chain reaction. Localization of uPA within normal and pancreatic tumor sections was subsequently confirmed using immunohistochemistry. Results: The median and range of the ratios of uPA mRNA measures between tumor tissue and non-involved pancreatic tissue was 17.1 (1.4-653.6) for pancreatic adenocarcinoma (P < 0.001), 3.9 (0.7-7.7) for ampullary carcinoma (P = 0.055) and 1.9 (0.6-5.9) for mucinous cystadenoma tissue (P = 0.052). uPA low tumors were associated with an exuberant stromal reaction, whereas uPA high tumors showed little stromal response. Immunohistochemistry confirmed that uPA protein was more prevalent in pancreatic adenocarcinoma tissue than in normal tissue and that it was membrane-bound. uPA mRNA expression was significantly associated with poorly differentiated pancreatic cancers (P < 0.05) and positively associated with tumor stage. Conclusions: These observations suggest that significant overexpression of uPA correlates closely to the rapid progression and invasiveness of pancreatic cancer and that uPA may provide a future therapeutic target for pancreatic cancer treatment. © 2004 Blackwell Publishing Asia Pty Ltd.

DOI 10.1111/j.1440-1746.2004.03531.x
Citations Scopus - 23
2004 Scarlett CJ, O'leary MJ, Kee AJ, Nielsen A, Sevette A, Baxter RC, Smith RC, 'A study of parenteral versus enteral nutrition following caecal ligation and puncture in the rat: Influence on survival and tissue protein turnover', Clinical Nutrition, 23 1135-1145 (2004) [C1]

Background &amp; aims: Methods of nutritional management in abdominal sepsis remain controversial. Methods: Sprague Dawley rats were either fed via a central line in the right i... [more]

Background & aims: Methods of nutritional management in abdominal sepsis remain controversial. Methods: Sprague Dawley rats were either fed via a central line in the right internal jugular vein or duodenally via a gastrostomy tube, and were randomised to undergo either caecal ligation and puncture (CLP) or laparotomy only. Post-operatively, animals received either parenteral nutrition, enteral nutrition or saline only (parenteral and enteral nutrition protocols were isocaloric and isonitrogenous). After 72 h, fractional rate of protein synthesis (K s , %/day) was measured in gastrocnemius muscle and liver, and protein breakdown was measured in incubated epitrochlearis muscles. Serum insulin-like growth factor-I (IGF-I), acid-labile subunit (ALS) and IGF binding protein-1 (IGFBP-1) levels were determined by specific radioimmunoassay methods. Results: After CLP, when compared with starved animals, only enteral nutrition resulted in a significant decrease in survival to 7 h (P < 0.001). Parenteral nutrition, but not enteral nutrition, increased muscle (P=0.02) and liver (P < 0.001) K s , IGF-I (P < 0.001) and ALS levels (P < 0.001), whereas both parenteral and enteral nutrition reduced IGFBP-1 levels (P < 0.001). Neither enteral nor parenteral nutrition reduced protein breakdown in septic animals. Conclusions: In this model of severe abdominal sepsis where gut function cannot be assessed, enteral nutrition was associated with increased mortality and was less effective than parenteral nutrition in augmenting muscle and liver protein synthesis. © 2004 Elsevier Ltd. All rights reserved.

DOI 10.1016/j.clnu.2004.02.008
Citations Scopus - 5
2001 Scarlett CJ, Lin M, Aitken RJ, 'Actin polymerisation during morphogenesis of the acrosome as spermatozoa undergo epididymal maturation in the tammar wallaby (Macropus eugenii)', Journal of Anatomy, 198 93-101 (2001) [C1]
DOI 10.1017/S0021878200007299
Citations Scopus - 11Web of Science - 10
Co-authors John Aitken
Show 106 more journal articles

Review (3 outputs)

Year Citation Altmetrics Link
2011 Scarlett CJ, Biankin A, 'S100A2 (S100 calcium binding protein A2; 1q21)', Atlas of Genetics and Cytogenetics in Oncology and Haematology (2011) [D2]
2011 Scarlett CJ, Salisbury EL, Biankin AV, Kench J, 'Precursor lesions in pancreatic cancer: Morphological and molecular pathology', Pathology (2011) [D1]
DOI 10.1097/PAT.0b013e3283445e3a
Citations Scopus - 34Web of Science - 22
2011 Pajic M, Scarlett CJ, Chang DK, Sutherland RL, Biankin AV, 'Preclinical strategies to define predictive biomarkers for therapeutically relevant cancer subtypes', Human Genetics (2011) [D1]
DOI 10.1007/s00439-011-0990-0
Citations Scopus - 10Web of Science - 9

Conference (20 outputs)

Year Citation Altmetrics Link
2016 Goldsmith C, Bond D, Stathopoulos C, Roach P, Scarlett C, 'THE OLIVE PHENOLIC COMPOUNDS APIGENIN, LUTEOLIN AND OLEUROPEIN INDUCE CELL-CYCLE ARREST AND APOPTOSIS IN PANCREATIC CANCER CELLS IN VITRO', ASIA-PACIFIC JOURNAL OF CLINICAL ONCOLOGY (2016)
Co-authors Danielle Bond
2016 Jankowski H, Brzozowski J, Goldie B, Bond D, Munro B, Scarlett C, et al., 'PROSTATE CANCER EXTRACELLULAR VESICLES: FRIEND OR FOE', ASIA-PACIFIC JOURNAL OF CLINICAL ONCOLOGY (2016)
Co-authors Danielle Bond, Judith Weidenhofer
2016 Bond D, Thuong PT, Thiha D, Khoi NM, Cuong NM, Weidenhofer J, Scarlett C, 'PURE COMPOUNDS FROM VIETNAMESE MEDICINAL PLANTS SHOW PROMISING ANTI-PANCREATIC CANCER ACTIVITY IN VITRO', ASIA-PACIFIC JOURNAL OF CLINICAL ONCOLOGY (2016)
Co-authors Judith Weidenhofer, Danielle Bond
2016 Brzozowski J, Jankowski H, Munro B, Predebon M, Bond D, Scarlett C, et al., 'ALTERATIONS IN LIPID COMPOSITION OF NORMAL AND TUMOR-DERIVED PROSTATE EXTRACELLULAR VESICLES', ASIA-PACIFIC JOURNAL OF CLINICAL ONCOLOGY (2016)
Co-authors Judith Weidenhofer, Danielle Bond
2016 Jankowski H, Goldie B, Brzozowski J, Munro B, Bond D, Scarlett C, et al., 'TETRASPANIN CD9 MODIFIES EXTRACELLULAR VESICLE NUCLEIC ACID CARGO IN PROSTATE CELL LINES', ASIA-PACIFIC JOURNAL OF CLINICAL ONCOLOGY (2016)
Co-authors Judith Weidenhofer, Danielle Bond
2016 Pristijono P, Bowyer M, Scarlett C, Vuong Q, Stathopoulos C, Golding J, 'Effect of UV-C irradiation on sprouting of potatoes in storage' (2016)
Co-authors Michael Bowyer, Vanquan Vuong
2016 Thakur R, Pristijono P, Golding J, Stathopoulos C, Scarlett C, Bowyer M, Vuong Q, 'Evaluation of Rice starch-¿-carrageenan Film Properties in the Presence of Different Hydrophobic Plasticizers' (2016)
Co-authors Michael Bowyer, Vanquan Vuong
2015 Jankowski H, Goldie B, Brzozowski J, Bond D, Scarlett C, Skelding KA, Weidenhofer J, 'Differences in extracellular vesicle nucleic acid content show promise as prostate cancer biomarkers' (2015) [O1]
Co-authors Danielle Bond, Kathryn Skelding, Judith Weidenhofer
2015 Chantrill LA, Pinho A, Wu J, Giry-Latierre M, Mawson A, Rees M, et al., 'Expression of the axon guidance protein Robo1 in pancreatic ductal adenocarcinoma from smokers compared to nonsmokers', JOURNAL OF CLINICAL ONCOLOGY (2015) [E3]
2015 Pristijono P, Golding J, Bowyer M, Scarlett C, Stathopoulos, 'Postharvest UV-C irradiation improves the quality of tomatoes and limes' (2015)
Co-authors Michael Bowyer
2015 Brzozowski J, Coldie B, Jankowski H, Bond D, Scarlett C, Dun M, et al., 'THE EFFECTS OF ALTERED CD9 AND CD151 EXPRESSION ON PROSTATE EXOSOMES', ASIA-PACIFIC JOURNAL OF CLINICAL ONCOLOGY (2015) [E3]
Co-authors Kathryn Skelding, Matt Dun, Judith Weidenhofer, Danielle Bond
2015 Jankowski H, Goldie B, Brzozowski J, Bond D, Scarlett C, Skelding K, Weidenhofer J, 'PROSTATE CANCER BIOMARKERS: ARE EXTRACELLULAR VESICLES THE SOLUTION?', ASIA-PACIFIC JOURNAL OF CLINICAL ONCOLOGY (2015) [E3]
Co-authors Judith Weidenhofer, Kathryn Skelding, Danielle Bond
2015 Bond D, Turner A, Richmond R, Sadeqzadeh E, Vuong Q, Bhuyan D, et al., 'THE SEARCH FOR NOVEL TREATMENT AGENTS FOR PANCREATIC CANCER: TALES FROM THE LAND AND SEA', ASIA-PACIFIC JOURNAL OF CLINICAL ONCOLOGY (2015) [E3]
Co-authors Danielle Bond, Judith Weidenhofer, Jennette Sakoff, Michael Bowyer, Troy Gaston, Ian Vanaltena, Vanquan Vuong, Anita Chalmers
2015 Predebon M, Bond D, Brzozowski J, Jankowski H, Deane F, Tarleton M, et al., 'A BISPIDINONE ANALOGUE INDUCES AN APOPTOSIS-MEDIATED CYTOTOXIC EFFECT ON PANCREATIC CANCER CELLS IN VITRO', ASIA-PACIFIC JOURNAL OF CLINICAL ONCOLOGY (2015) [E3]
Co-authors Judith Weidenhofer, Michael Bowyer, Danielle Bond, Adam Mccluskey
2014 Shahbazi J, Scarlett CJ, Norris M, Liu B, Haber M, Tee AE, et al., 'Histone deacetylase 2 and N-Myc reduce p53 protein phosphorylation at serine 46 by repressing gene transcription of tumor protein 53-induced nuclear protein 1', CANCER RESEARCH (2014) [E3]
DOI 10.1158/1538-7445.AM2014-5138
2014 Sun Y, Liu PY, Scarlett CJ, Malyukova A, Liu B, Marshall GM, et al., 'Histone deacetylase 5 blocks neuroblastoma cell differentiation by forming a protein complex with N-Myc and repressing target gene transcription', INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE (2014) [E3]
2014 Faulkner S, Roselli S, Thorne RF, Scarlett CJ, Walker MM, Hondermarck H, 'PRONGF AND SORTILIN EXPRESSION AND FUNCTION IN PANCREATIC CANCER', ASIA-PACIFIC JOURNAL OF CLINICAL ONCOLOGY (2014) [E3]
Co-authors Rick Thorne, Hubert Hondermarck, Marjorie Walker
2014 Goldsmith CD, Vuong QV, Sadeqzadeh E, Stathopoulos CE, Roach PD, Scarlett CJ, 'ANTI-PROLIFERATIVE CAPACITY OF OLEUROPEIN RICH OLIVE LEAF EXTRACTS AGAINST PANCREATIC CANCER CELLS', ASIA-PACIFIC JOURNAL OF CLINICAL ONCOLOGY (2014) [E3]
Co-authors Paul Roach, Vanquan Vuong
2014 Sadeqzadeh E, Vuong QV, Goldsmith CD, Nguyen VT, Bhuyan DJ, Trung TD, et al., 'A NATURAL PRODUCT DRUG DISCOVERY PIPELINE FOR NOVEL PANCREATIC CANCER THERAPIES: A NEW CANCER RESEARCH HUB FOR THE HUNTER REGION OF NSW', ASIA-PACIFIC JOURNAL OF CLINICAL ONCOLOGY (2014) [E3]
Co-authors Vanquan Vuong, Ian Vanaltena, Judith Weidenhofer, Rick Thorne, Michael Bowyer, Troy Gaston, Natalie Moltschaniwskyj, Anita Chalmers
2012 Chantrill L, Gill A, Johns A, Wu J, Scarlett CJ, Biankin A, Australian Pancreatic Cancer Genome Initiative, 'The outliers of pancreatic cancer: Preliminary data for timeless expression as a prognostic biomarker', Asia-Pacific Journal of Clinical Oncology: Special Issue: Medical Oncology Group of Australia Incorporated Annual Scientific Meeting, Program and Abstracts: Targeting Cancer from Diagnosis to Cure (2012) [E3]
Show 17 more conferences
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Grants and Funding

Summary

Number of grants 28
Total funding $5,331,500

Click on a grant title below to expand the full details for that specific grant.


20172 grants / $203,008

HMRI MRSP Cancer Research Program$190,008

Funding body: Hunter Medical Research Institute

Funding body Hunter Medical Research Institute
Project Team Conjoint Professor Stephen Ackland, Professor Xu Dong Zhang, Laureate Professor Rodney Scott, Doctor Nikki Verrills, Conjoint Associate Professor Jarad Martin, Doctor Steve Smith, Associate Professor Christine Paul, Conjoint Professor Peter Greer, Conjoint Associate Professor Anthony Proietto, Doctor Fiona Day, Associate Professor Christopher Scarlett
Scheme NSW MRSP Infrastructure Grant
Role Investigator
Funding Start 2017
Funding Finish 2017
GNo G1700603
Type Of Funding Other Public Sector - State
Category 2OPS
UON Y

Subproject: HMRI MRSP Cancer Research Program$13,000

Funding body: Hunter Medical Research Institute

Funding body Hunter Medical Research Institute
Project Team Associate Professor Christopher Scarlett
Scheme NSW MRSP Infrastructure Grant
Role Lead
Funding Start 2017
Funding Finish 2017
GNo GS170005
Type Of Funding Other Public Sector - State
Category 2OPS
UON Y

20162 grants / $28,750

Circulating exosomes in pancreatic cancer as a source of novel diagnostic biomarkers$23,750

Funding body: Hunter Medical Research Institute

Funding body Hunter Medical Research Institute
Project Team Associate Professor Christopher Scarlett, Doctor Jude Weidenhofer, Doctor Kelly Kiejda
Scheme Project Grant
Role Lead
Funding Start 2016
Funding Finish 2016
GNo G1601070
Type Of Funding Grant - Aust Non Government
Category 3AFG
UON Y

Equipment Grant: Developing Synthetic Exosomes to Target and Deliver Anti-Cancer Agents to Prostate Cancer$5,000

Funding body: Hunter Medical Research Institute

Funding body Hunter Medical Research Institute
Project Team Mr Joshua Brzozowski, Doctor Jude Weidenhofer, Doctor Kathryn Skelding, Associate Professor Christopher Scarlett
Scheme Project Grant
Role Investigator
Funding Start 2016
Funding Finish 2016
GNo G1601042
Type Of Funding Grant - Aust Non Government
Category 3AFG
UON Y

20153 grants / $157,938

Live cell imager for enhancement of pre-clinical cancer studies in the Hunter Translational Cancer Research Centre$124,938

Funding body: Cancer Institute NSW

Funding body Cancer Institute NSW
Project Team Conjoint Professor Stephen Ackland, Doctor Kelly Kiejda, Associate Professor Kevin Spring, Professor Xu Dong Zhang, Associate Professor Deborah Marsh, Associate Professor Christopher Scarlett, Doctor Pradeep Tanwar, Doctor Kathryn Skelding, Doctor Rick Thorne, Doctor Nikola Bowden
Scheme Research Equipment Grant
Role Investigator
Funding Start 2015
Funding Finish 2016
GNo G1500598
Type Of Funding Other Public Sector - State
Category 2OPS
UON Y

Live cell imager for enhancement of pre-clinical cancer studies in the Hunter Translational Cancer Research Centre$25,000

Funding body: University of Newcastle

Funding body University of Newcastle
Project Team Conjoint Professor Stephen Ackland, Doctor Kelly Kiejda, Associate Professor Kevin Spring, Professor Xu Dong Zhang, Associate Professor Deborah Marsh, Associate Professor Christopher Scarlett, Doctor Pradeep Tanwar, Doctor Kathryn Skelding, Doctor Rick Thorne, Doctor Nikola Bowden
Scheme Equipment Grant
Role Investigator
Funding Start 2015
Funding Finish 2015
GNo G1500953
Type Of Funding Internal
Category INTE
UON Y

Novel Drug Leads for Pancreatic Cancer Treatment$8,000

Funding body: Calvary Mater Newcastle

Funding body Calvary Mater Newcastle
Project Team Associate Professor Christopher Scarlett, Dr Jennette Sakoff
Scheme Project Grant
Role Lead
Funding Start 2015
Funding Finish 2015
GNo G1500436
Type Of Funding Contract - Aust Non Government
Category 3AFC
UON Y

20148 grants / $2,171,048

Food & Beverage Supply Chain Optimisation Industrial Transformation Training Centre$1,243,927

Funding body: ARC (Australian Research Council)

Funding body ARC (Australian Research Council)
Project Team Professor Rick Middleton, Professor Regina Berretta, Associate Professor Michael Bowyer, Doctor Ali Eshragh, Associate Professor Behnam Fahimnia, Professor Mathieu Savelsbergh, Professor Natashia Boland, Dr Costas Stathopoulos, Professor John Bartholdi, Doctor Simon Dunstall, Mrs Carlee McGowan, Mr Robert McMahon, Mr Tim Norris, Mr Robert Scoines, Associate Professor Christopher Scarlett, Doctor Quan Vuong
Scheme Industrial Transformation Training Centres
Role Investigator
Funding Start 2014
Funding Finish 2016
GNo G1301004
Type Of Funding Aust Competitive - Commonwealth
Category 1CS
UON Y

ARC ITTC ELS subaccount$629,422

Funding body: ARC (Australian Research Council)

Funding body ARC (Australian Research Council)
Project Team Associate Professor Michael Bowyer, Associate Professor Christopher Scarlett
Scheme Industrial Transformation Training Centres
Role Investigator
Funding Start 2014
Funding Finish 2016
GNo GS150004
Type Of Funding Aust Competitive - Commonwealth
Category 1CS
UON Y

Visualisation of microparticles for development of biomarkers and targeted drug delivery mechanisms$125,199

Funding body: Cancer Institute NSW

Funding body Cancer Institute NSW
Project Team Associate Professor Christopher Scarlett, Doctor Kathryn Skelding, Doctor Jude Weidenhofer, Doctor Matt Dun, Doctor Kelly Kiejda, Professor Adam McCluskey, Ms Elham Sadeqzadeh, Professor Hubert Hondermarck, Doctor Rick Thorne, Laureate Professor Rodney Scott
Scheme Research Equipment Grant
Role Lead
Funding Start 2014
Funding Finish 2014
GNo G1400627
Type Of Funding Other Public Sector - State
Category 2OPS
UON Y

Food & Beverage Supply Chain Optimisation Industrial Transformation Training Centre$90,000

Funding body: Coca Cola Amatil (Australia)

Funding body Coca Cola Amatil (Australia)
Project Team Professor Rick Middleton, Professor Regina Berretta, Associate Professor Michael Bowyer, Doctor Ali Eshragh, Associate Professor Christopher Scarlett, Doctor Masoud Talebian, Associate Professor Behnam Fahimnia, Professor Mathieu Savelsbergh, Professor Natashia Boland, Dr Costas Stathopoulos, Professor John Bartholdi, Doctor Simon Dunstall, Mrs Carlee McGowan, Mr Robert McMahon, Mr Robert Scoines, Mr Tim Norris
Scheme Industrial Transformation Training Centres Partner Funding
Role Investigator
Funding Start 2014
Funding Finish 2016
GNo G1301129
Type Of Funding Grant - Aust Non Government
Category 3AFG
UON Y

Food & Beverage Supply Chain Optimisation Industrial Transformation Training Centre$30,000

Funding body: Sanitarium Health and Wellbeing Company

Funding body Sanitarium Health and Wellbeing Company
Project Team Professor Rick Middleton, Professor Regina Berretta, Associate Professor Michael Bowyer, Doctor Ali Eshragh, Associate Professor Christopher Scarlett, Doctor Masoud Talebian, Associate Professor Behnam Fahimnia, Professor Mathieu Savelsbergh, Professor Natashia Boland, Dr Costas Stathopoulos, Professor John Bartholdi, Doctor Simon Dunstall, Mrs Carlee McGowan, Mr Robert McMahon, Mr Robert Scoines, Mr Tim Norris
Scheme Industrial Transformation Training Centres Partner Funding
Role Investigator
Funding Start 2014
Funding Finish 2016
GNo G1301130
Type Of Funding Grant - Aust Non Government
Category 3AFG
UON Y

Food & Beverage Supply Chain Optimisation Industrial Transformation Training Centre$30,000

Funding body: Sunrice

Funding body Sunrice
Project Team Professor Rick Middleton, Professor Regina Berretta, Associate Professor Michael Bowyer, Doctor Ali Eshragh, Associate Professor Christopher Scarlett, Doctor Masoud Talebian, Associate Professor Behnam Fahimnia, Professor Mathieu Savelsbergh, Professor Natashia Boland, Dr Costas Stathopoulos, Professor John Bartholdi, Doctor Simon Dunstall, Mrs Carlee McGowan, Mr Robert McMahon, Mr Robert Scoines, Mr Tim Norris
Scheme Industrial Transformation Training Centres Partner Funding
Role Investigator
Funding Start 2014
Funding Finish 2016
GNo G1301131
Type Of Funding Grant - Aust Non Government
Category 3AFG
UON Y

Identification and evaluation of anti-pancreatic cancer activity of cytotoxic compounds extracted from Australian sea sponges: a pilot study$20,500

Funding body: Hunter Medical Research Institute

Funding body Hunter Medical Research Institute
Project Team Associate Professor Christopher Scarlett, Doctor Quan Vuong, Doctor Jude Weidenhofer, Doctor Rick Thorne, Associate Professor Michael Bowyer, Doctor Troy Gaston
Scheme Project Grant
Role Lead
Funding Start 2014
Funding Finish 2015
GNo G1401452
Type Of Funding Grant - Aust Non Government
Category 3AFG
UON Y

2013 Vice Chancellor's Award for Research Excellence - Regional$2,000

Funding body: University of Newcastle

Funding body University of Newcastle
Project Team Associate Professor Christopher Scarlett
Scheme Award for Research Excellence
Role Lead
Funding Start 2014
Funding Finish 2014
GNo G1301445
Type Of Funding Internal
Category INTE
UON Y

20133 grants / $54,303

Gastro-pancreatic cancer research$32,050

Funding body: Terrigal Trotters Inc. Running Club

Funding body Terrigal Trotters Inc. Running Club
Project Team Associate Professor Christopher Scarlett
Scheme Bay to Bay Running Festival
Role Lead
Funding Start 2013
Funding Finish 2017
GNo G1301010
Type Of Funding Grant - Aust Non Government
Category 3AFG
UON Y

Extraction and identification of cytotoxic compounds from Australian sea sponges as novel therapeutic agents for pancreatic cancer.$14,749

Funding body: University of Newcastle - Faculty of Science & IT

Funding body University of Newcastle - Faculty of Science & IT
Project Team Associate Professor Christopher Scarlett, Doctor Quan Vuong
Scheme Strategic Small Grant
Role Lead
Funding Start 2013
Funding Finish 2013
GNo G1401071
Type Of Funding Internal
Category INTE
UON Y

Faculty Visiting Fellowship 2013$7,504

Funding body: University of Newcastle - Faculty of Science & IT

Funding body University of Newcastle - Faculty of Science & IT
Project Team Associate Professor Christopher Scarlett
Scheme Visiting Fellowship
Role Lead
Funding Start 2013
Funding Finish 2013
GNo G1401145
Type Of Funding Internal
Category INTE
UON Y

20125 grants / $583,826

The therapeutic potential of targeting S100A2 calcium-binding protein in pancreatic cancer$177,451

Funding body: Cancer Australia

Funding body Cancer Australia
Project Team Associate Professor Christopher Scarlett
Scheme Priority-driven Collaborative Cancer Research Scheme
Role Lead
Funding Start 2012
Funding Finish 2014
GNo G1200404
Type Of Funding Aust Competitive - Commonwealth
Category 1CS
UON Y

The therapeutic potential of targeting S100A2 calcium-binding protein in pancreatic cancer$177,450

Pancreatic cancer (PC) is the fourth leading cause of cancer death in Western societies with an overall 5-year survival rate of less than 5%. Consequently, there is an urgent need to develop novel therapeutic strategies. Analysis of clinical material by our group demonstrate that S100A2 calcium-binding protein defines a specific pancreatic cancer phenotype, and suggests that S100A2 calcium-binding protein may either play a key role, or is a surrogate marker of a metastatic phenotype. In addition, our preliminary experiments suggest that S100A2 does play a key role in the metastatic process in PC. Gain of function studies showed increased invasion in vitro, and in vivo analyses demonstrated marked increased potential to develop liver metastases, the major cause of death for pancreatic cancer. Consequently, the overall aim of this proposal is to test the hypothesis that S100A2 plays a role in pancreatic cancer metastasis, and may be suitable as a therapeutic target in pancreatic cancer.

Funding body: Cure Cancer Australia Foundation

Funding body Cure Cancer Australia Foundation
Project Team

Chris Scarlett

Scheme Research Grant
Role Lead
Funding Start 2012
Funding Finish 2013
GNo
Type Of Funding Not Known
Category UNKN
UON N

Novel Targeted Therapies for Pancreatic Cancer$149,877

Funding body: Cancer Institute NSW

Funding body Cancer Institute NSW
Project Team Associate Professor Christopher Scarlett
Scheme Career Development Fellowship
Role Lead
Funding Start 2012
Funding Finish 2012
GNo G1200531
Type Of Funding Other Public Sector - State
Category 2OPS
UON Y

Establishment of a pancreatic cancer research program for screening the activity and efficacy of natural bioactive compounds$74,048

Funding body: Ramaciotti Foundations

Funding body Ramaciotti Foundations
Project Team Associate Professor Christopher Scarlett
Scheme Establishment Grant
Role Lead
Funding Start 2012
Funding Finish 2013
GNo G1200739
Type Of Funding Grant - Aust Non Government
Category 3AFG
UON Y

Project support to develop pancreatic cancer research within the School$5,000

Funding body: University of Newcastle

Funding body University of Newcastle
Project Team Associate Professor Christopher Scarlett
Scheme New Staff Grant
Role Lead
Funding Start 2012
Funding Finish 2012
GNo G1200559
Type Of Funding Internal
Category INTE
UON Y

20111 grants / $344,208

Targeting histone deacetylases for the therapy of Myc-induced malignancies$344,208

BACKGROUND: N-Myc and c-Myc oncoproteins are over-expressed in tumour tissues from neuroblastoma (N-Myc) and pancreatic cancer (c-Myc) patients, induce oncogenesis by modulating gene transcription (CIA, PNAS 2007), and are stabilized when phosphorylated by ERK. By repressing target gene transcription, histone deacetylase 2 (HDAC2) induces proliferation and blocks apoptosis in cancer cells, and HDAC5 blocks stem cell differentiation. HDAC inhibitors including panobinostat are highly effective anti-cancer agents in clinical trials. However, some patients are insensitive. The ERK inhibitor PD0325901 reduces Myc expression, induces tumour cell growth arrest and apoptosis, and blocks tumour growth in mice and in clinical trials. PRELIMINARY DATA: We have found in neuroblastoma and pancreatic cancer cells that Myc oncoproteins up-regulate HDAC2 and HDAC5 expression, that HDAC2 promotes cell proliferation and HDAC5 blocks cell differentiation, and that HDAC2 and Myc commonly repress the transcription of a subset of genes and form a protein complex. Moreover, HDAC2 protein expression is up-regulated in Myc over-expressing neuroblastoma and pancreatic cancer tissues, and panobinostat and PD0325901 exert synergistic anti-cancer effects in vitro. AIMS: To define the roles of HDAC2 up-regulation in Myc-induced transcriptional repression, cell proliferation and/or apoptosis, HDAC5 up-regulation in Myc-induced cell differentiation block, HDAC2 and HDAC5 in the initiation and progression of neuroblastoma and pancreatic cancer in vivo, and the anti-cancer efficacy of panobinostat and PD0325901 combination therapy against neuroblastoma and pancreatic cancer in vitro and in vivo. SIGNIFICANCE: This innovative project addresses an unrecognised interaction between HDAC2, HDAC5 and Myc. Successful completion of this project will provide direct evidence for clinical trials of the combination therapy for the treatment of neuroblastoma and pancreatic cancer patients.

Funding body: NHMRC (National Health & Medical Research Council)

Funding body NHMRC (National Health & Medical Research Council)
Project Team

Dr Tao Liu

Scheme Project Grant
Role Investigator
Funding Start 2011
Funding Finish 2013
GNo
Type Of Funding Not Known
Category UNKN
UON N

20102 grants / $919,181

Novel Targeted Therapies for Pancreatic Cancer$599,681

Funding body: Cancer Institute NSW

Funding body Cancer Institute NSW
Project Team

Chris Scarlett

Scheme Career Development Fellowship
Role Lead
Funding Start 2010
Funding Finish 2012
GNo
Type Of Funding Not Known
Category UNKN
UON N

Targeting Myc onco-protein degradation for the treatment of Myc-induced malignancies$319,500

Neuroblastoma is the commonest solid tumour in early childhood. Pancreatic cancer is the fourth leading cause of cancer death in adults with a 5-year survival rate of less than 5%. We will define how proteins called histone deacetylases promote cancer initiation and progression, and whether specific inhibitors of the histone deacetylases exert anti-cancer effects in animal models of neuroblastoma and pancreatic cancer.

Funding body: Cancer Council NSW

Funding body Cancer Council NSW
Project Team

Dr Tao Liu

Scheme Research Grant
Role Investigator
Funding Start 2010
Funding Finish 2012
GNo
Type Of Funding Not Known
Category UNKN
UON N

20091 grants / $330,000

Continuing infrastructure support for the ACRF Genomics Facility$330,000

Continuing infrastructure support for the ACRF Genomics Facility

Funding body: Cancer Instititue NSW

Funding body Cancer Instititue NSW
Project Team

Prof Sue Clark

Scheme Research Infrastructure Grants
Role Investigator
Funding Start 2009
Funding Finish 2011
GNo
Type Of Funding Not Known
Category UNKN
UON N

20071 grants / $539,238

Adult Haematopoietic Stem Cells in The Evolution of Pancreatic Cancer$539,238

This project sought to define the role for bone marrow-derived cells in normal pancreatic development, pancreatic injury and regeneration and pancreatic cancer.

Funding body: Cancer Instititue NSW

Funding body Cancer Instititue NSW
Project Team

Chris Scarlett

Scheme Early Career Fellowship
Role Lead
Funding Start 2007
Funding Finish 2009
GNo
Type Of Funding Not Known
Category UNKN
UON N
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Research Supervision

Number of supervisions

Completed1
Current16

Total current UON EFTSL

PhD5.85

Current Supervision

Commenced Level of Study Research Title Program Supervisor Type
2017 PhD Synthesis, Biological Activities of a,ß-unsaturated Carbonyl Compounds PhD (Chemistry), Faculty of Science, The University of Newcastle Co-Supervisor
2016 PhD Targeting the S100A2 Axis as a Novel Therapeutic Target for Pancreatic Cancer PhD (Biological Sciences), Faculty of Science, The University of Newcastle Principal Supervisor
2015 PhD Aqueous Polyphenol Extracts of Lemon (Citrus limon L.) Waste Generated by Lemon Juice Industry: Optimisation of Extraction & Encapsulation PhD (Food Science), Faculty of Science, The University of Newcastle Co-Supervisor
2015 PhD Identification and Isolation of Bioactive Compounds from Selected Australian and Vietnamese Flora as Novel Agents for Prevention and Treatment of Pancreatic Cancer PhD (Food Science), Faculty of Science, The University of Newcastle Co-Supervisor
2015 PhD Extraction, Isolation and Identification of Phytochemicals from the Tuckeroo (Cupaniopsis anacardioides) fruit as Novel Anti-pancreatic Cancer Agents PhD (Food Science), Faculty of Science, The University of Newcastle Principal Supervisor
2015 PhD Maximizing the Recovery of Bioactive compounds from Banana (Muss Cavendish) peel for Utilization as a Food Ingredient PhD (Food Science), Faculty of Science, The University of Newcastle Co-Supervisor
2015 PhD Anticancer Compounds from Plants Against Pancreatic Cancer Cells: Extraction, Purification and Characterization PhD (Food Science), Faculty of Science, The University of Newcastle Principal Supervisor
2015 PhD Circulating Exosomes in Pancreatic Cancer as a Source of Novel Diagnostic Biomarkers PhD (Biological Sciences), Faculty of Science, The University of Newcastle Principal Supervisor
2015 PhD Edible Films and Coatings for Improving Postharvest Shelf Life of Horticulture Produce PhD (Food Science), Faculty of Science, The University of Newcastle Co-Supervisor
2014 PhD Identification of Biomarkers and Novel Targets for Prostate Cancer from Exosomes PhD (Medical Biochemistry), Faculty of Health and Medicine, The University of Newcastle Co-Supervisor
2014 PhD Developing Synthetic Exosomes to Target and Deliver Anti-Cancer Agents to Prostate Cancer Cells PhD (Medical Biochemistry), Faculty of Health and Medicine, The University of Newcastle Co-Supervisor
2014 PhD Evaluation of Edible Coating Application on Different Fruits PhD (Food Science), Faculty of Science, The University of Newcastle Principal Supervisor
2014 PhD Brown Algae as a Source of Bioactive Compounds for Pancreatic Cancer Treatment PhD (Food Science), Faculty of Science, The University of Newcastle Principal Supervisor
2014 PhD Bioactive Compounds, Antioxidant and Cytotoxic Capacities of Two Vietnamese Medicinal Plants: Phyllanthus Amarus and Paramignya Trimera PhD (Food Science), Faculty of Science, The University of Newcastle Principal Supervisor
2014 PhD Phytochemicals Derived From Australian Eucalypts as Anticancer Agents for Pancreatic Malignancies PhD (Food Science), Faculty of Science, The University of Newcastle Principal Supervisor
2013 PhD Olive Waste Products as a Source of Phenolic Compounds with Chemotherapeutic and Chemopreventative Efficacy Against Pancreatic Cancer PhD (Food Science), Faculty of Science, The University of Newcastle Principal Supervisor

Past Supervision

Year Level of Study Research Title Program Supervisor Type
2017 PhD Development of Valid Performance Testing Protocols for Mountain Bikers PhD (Exercise & Sport Science), Faculty of Science, The University of Newcastle Principal Supervisor
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Associate Professor Christopher Scarlett

Position

Associate Professor
Food Science & Human Nutrition
School of Environmental and Life Sciences
Faculty of Science

Focus area

Applied Sciences

Contact Details

Email c.scarlett@newcastle.edu.au
Phone (02) 4348 4680

Office

Room SO E1.46
Building Science Offices.
Location Ourimbah
10 Chittaway Road
Ourimbah, NSW 2258
Australia
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