| 2016 |
Gibb Z, Lambourne SR, Curry BJ, Hall SE, Aitken RJ, 'Aldehyde Dehydrogenase Plays a Pivotal Role in the Maintenance of Stallion Sperm Motility', BIOLOGY OF REPRODUCTION, 94 (2016) [C1]
|
|
|
| 2015 |
Swegen A, Curry BJ, Gibb Z, Lambourne SR, Smith ND, Aitken RJ, 'Investigation of the stallion sperm proteome by mass spectrometry', Reproduction, 149 235-244 (2015) [C1]
© 2015 Society for Reproduction and Fertility. Stallion spermatozoa continue to present scientific and clinical challenges with regard to the biological mechanisms responsible for... [more] © 2015 Society for Reproduction and Fertility. Stallion spermatozoa continue to present scientific and clinical challenges with regard to the biological mechanisms responsible for their survival and function. In particular, deeper understanding of sperm energy metabolism, defence against oxidative damage and cell-cell interactions should improve fertility assessment and the application of advanced reproductive technologies in the equine species. In this study, we used highly sensitive LC-MS/MS technology and sequence database analysis to identify and characterise the proteome of Percoll-isolated ejaculated equine spermatozoa, with the aim offurthering our understanding of this cell's complex biological machinery. We were able to identify 9883 peptides comprising 1030 proteins, which were subsequently attributed to 975 gene products. Gene ontology analysis for molecular and cellular processes revealed new information about the metabolism, antioxidant defences and receptors of stallion spermatozoa. Mitochondrial proteins and those involved in catabolic processes constituted dominant categories. Several enzymes specific to ß-oxidation of fatty acids were identified, and further experiments were carried out to ascertain their functional significance. Inhibition of carnitine palmitoyl transferase 1, a rate-limiting enzyme of ß-oxidation, reduced motility parameters, indicating that b-oxidation contributes to maintenance of motility in stallion spermatozoa.
|
|
|
| 2015 |
Aitken JB, Naumovski N, Curry B, Grupen CG, Gibb Z, Aitken RJ, 'Characterization of an L-amino acid oxidase in equine spermatozoa.', Biol Reprod, 92 125 (2015) [C1]
|
|
|
| 2015 |
Houston B, Curry B, Aitken RJ, 'Human spermatozoa possess an IL4I1 L-amino acid oxidase with a potential role in sperm function', Reproduction, 149 587-596 (2015) [C1]
© 2015 Society for Reproduction and Fertility. Reactive oxygen species (ROS) are known to play an important role in the regulation of human sperm function. In this study, we demon... [more] © 2015 Society for Reproduction and Fertility. Reactive oxygen species (ROS) are known to play an important role in the regulation of human sperm function. In this study, we demonstrate for the first time that human spermatozoa possess interleukin-induced gene 1 (IL4I1), an L-amino acid oxidase (LAAO) which is capable of generating ROS on exposure to aromatic amino acids in the presence of oxygen. The preferred substrates were found to be phenylalanine and tryptophan while the enzyme was located in the acrosomal region and midpiece of these cells. In contrast to equine and bovine spermatozoa, enzyme activity was lost as soon as the spermatozoa became non-viable. On a cell-to-cell basis human spermatozoa were also shown to generate lower levels of hydrogen peroxide than their equine counterparts on exposure to phenylalanine. Stimulation of LAAO activity resulted in the induction of several hallmarks of capacitation including tyrosine phosphorylation of the sperm flagellum and concomitant activation of phospho-SRC expression. In addition, stimulation of LAAO resulted in an increase in the levels of acrosomal exocytosis in both the presence and absence of progesterone stimulation, via mechanisms that could be significantly reversed by the presence of catalase. As is often the case with free radical-mediated phenomena, prolonged exposure of human spermatozoa to phenylalanine resulted in the stimulation of apoptosis as indicated by significant increases in mitochondrial superoxide generation and the activation of intracellular caspases. These results confirm the existence of an LAAO in human spermatozoa with a potential role in driving the redox regulation of sperm capacitation and acrosomal exocytosis.
|
|
|
| 2013 |
Smith TB, Dun MD, Smith ND, Curry BJ, Connaughton HS, Aitken RJ, 'The presence of a truncated base excision repair pathway in human spermatozoa that is mediated by OGG1', JOURNAL OF CELL SCIENCE, 126 1488-1497 (2013) [C1]
|
|
|
| 2011 |
Aitken RJ, Curry BJ, 'Redox regulation of human sperm function: From the physiological control of sperm capacitation to the etiology of infertility and DNA damage in the germ line', Antioxidants & Redox Signaling, 14 367-381 (2011) [C1]
|
|
|
| 2010 |
Pujianto DA, Curry BJ, Aitken RJ, 'Prolactin exerts a prosurvival effect on human spermatozoa via mechanisms that involve the stimulation of Akt phosphorylation and suppression of caspase activation and capacitation', Endocrinology, 151 1269-1279 (2010) [C1]
|
|
|
| 2009 |
Baker MA, Hetherington L, Curry BJ, Aitken RJ, 'Phosphorylation and consequent stimulation of the tyrosine kinase c-Abl by PKA in mouse spermatozoa: Its implications during capacitation', Developmental Biology, 333 57-66 (2009) [C1]
|
|
|
| 2006 |
Curry BJ, Holt JE, McLaughlin EA, Aitken RJ, 'Characterization of structure and expression of the Dzip1 gene in the rat and mouse', Genomics, 87 275-285 (2006) [C1]
|
|
|
| 2005 |
Baker MA, Krutskikh A, Curry BJ, Hetherington L, Aitken RJ, 'Identification of cytochrome-b5 reductase as the enzyme responsible for NADH-dependent lucigenin chemiluminescence in human spermatozoa', Biology of Reproduction, 73 334-342 (2005) [C1]
|
|
|
| 2004 |
Baker MA, Krutskikh A, Curry BJ, McLaughlin EA, Aitken RJ, 'Identification of Cytochrome P450-Reductase as the Enzyme Responsible for NADPH-Dependent Lucigenin and Tetrazolium Salt Reduction in Rat Epididymal Sperm Preparations', Biology of Reproduction, 71 307-318 (2004) [C1]
|
|
|
| 2004 |
Curry BJ, Su H, Law EG, McLaughlin EA, Nixon B, Aitken RJ, 'Identification of RARhoGAP, a novel putative RhoGAP gene expressed in male germ cells', Genomics, 84 406-418 (2004) [C1]
|
|
|
| 2004 |
Curry BJ, Roman SD, Wallace C, Scott RJ, Miriami E, Aitken RJ, 'Identification and characterisation of a novel splice variant of mouse and rat cytochrome b5/cytochrome b5 reductase', Genomics, 83 425-438 (2004) [C1]
|
|
|
| 2003 |
Aitken RJ, Ryan AL, Curry BJ, Baker MA, 'Multiple forms of redox activity in populations of human spermatozoa', Molecular Human Reproduction, 9 645-661 (2003) [C1]
|
|
|
| 2001 |
Curry JB, Myers K, Hersey P, 'Utility of Tests for Circulating Melanoma Cells in Identifying Patients Who Develop Recurrent Melanoma', Cancer Research, 158 211-230 (2001) [C1]
|
|
|
| 1999 |
Curry BJ, Myers K, Hersey P, 'MART-1 is expressed less frequently on circulating melanoma cells in patients who develop distant compared with locoregional metastases', JOURNAL OF CLINICAL ONCOLOGY, 17 2562-2571 (1999)
|
|
|
| 1999 |
Curry BJ, Farrelly M, Hersey P, 'Evaluation of S-100 beta assays for the prediction of recurrence and prognosis in patients with AJCC stage I-III melanoma', MELANOMA RESEARCH, 9 557-567 (1999)
|
|
|
| 1998 |
Curry BJ, Myers K, Hersey P, 'Polymerase chain reaction detection of melanoma cells in the circulation: Relation to clinical stage, surgical treatment, and recurrence from melanoma', JOURNAL OF CLINICAL ONCOLOGY, 16 1760-1769 (1998)
|
|
|
| 1996 |
Curry BJ, Smith MJ, Hersey P, 'Detection and quantitation of melanoma cells in the circulation of patients', MELANOMA RESEARCH, 6 45-54 (1996)
|
|
|
