Dr Annalucia Darbey
Postdoctoral Research Associate
School of Environmental and Life Sciences
My first experience of research began at the University of Edinburgh where I undertook my degree in Masters in Research in Reproductive Sciences. During this time, I was afforded the opportunity to work under the supervision of Professor Lee Smith investigating the roles of Sertoli cells and using gene editing technologies such as Crispr/Cas9 for the study of Androgen Receptor.
I completed my PhD in 2018, continuing with Professor Lee Smith, as part of the Tissue Repair Programme at the University of Edinburgh. My PhD project was focussed upon the targeting and repair of adult testicular somatic cells through viral gene therapy. Following recruitment to The University of Newcastle as a Postdoctoral Research Associate in 2019, I now continue to investigate deliverable transgenics and their potential applications in the adult testis and wider endocrine systems. Development of this novel technology will permit the investigation of the genetics underpinning male fertility and androgen production and will lead to the development of novel bespoke therapeutics and contraceptives fulfilling a currently unmet need for millions of men worldwide.
Current Research Themes
My current research themes are focussed upon the development of novel technologies for both the investigation and the treatment of male reproductive disorders, including the investigation of alternative options for long lasting male contraceptives. To complement this, I am also investigating novel in vitro models of endocrine organs, which may prove vital for the investigation of new therapeutics in human cells. Therefore, I have a keen interest in deliverable transgenics, viral vectors, nanoparticle technology and 3D printing and biofabrication.
Whilst at the University of Edinburgh, I was involved in the teaching and supervision of undergraduate students; in particular on the Rebroductive Biology undergraduate course. This included the direct demonstration of techniques in practical lab sessions as well as supervision of tutorial groups through group assignments. Since being appointed at the University of Newcastle, I have lectured on Reproductive Physiology and Development Modules (BIOL3020) and supervised Biotechnology students on 3rd year placement projects.
- Doctor of Philosophy, University of Edinburgh - Scotland
- 3D Printing
- Deliverable Transgenics
- Gene Therapy
- Male Reproductive Biology
- Viral Vectors
- English (Mother)
Fields of Research
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For publications that are currently unpublished or in-press, details are shown in italics.
Chapter (1 outputs)
Rebourcet D, Darbey A, Curley M, O Shaughnessy P, Smith LB, 'Testicular cell selective ablation using diphtheria toxin receptor transgenic mice', Sertoli Cells: Methods and Protocols, Humana Press, New York, NY 203-228 (2018) [B1]
Journal article (4 outputs)
Darbey A, Smith LB, 'Deliverable transgenics & gene therapy possibilities for the testes', Molecular and Cellular Endocrinology, 468 81-94 (2018) [C1]
Curley M, Milne L, Smith S, Atanassova N, Rebourcet D, Darbey A, et al., 'Leukemia Inhibitory Factor-Receptor is Dispensable for Prenatal Testis Development but is Required in Sertoli cells for Normal Spermatogenesis in Mice', SCIENTIFIC REPORTS, 8 (2018) [C1]
Rebourcet D, Darbey A, Monteiro A, Soffientini U, Tsai YT, Handel I, et al., 'Sertoli Cell Number Defines and Predicts Germ and Leydig Cell Population Sizes in the Adult Mouse Testis', ENDOCRINOLOGY, 158 2955-2969 (2017) [C1]
Patel SH, O'Hara L, Atanassova N, Smith SE, Curley MK, Rebourcet D, et al., 'Low-dose tamoxifen treatment in juvenile males has long-term adverse effects on the reproductive system: implications for inducible transgenics.', Scientific Reports, 7 (2017) [C1]
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