Dr Jenny Irwin
Dr Jenny Irwin has an extensive background in chemistry having worked for many years in industrial and academic research. Her fundamental training is as a bio-inorganic chemist. Dr Irwin holds a BSc Hons Class I (1993) from the University of Newcastle and PhD in bioinorganic chemistry from the University of Sydney (1998) having worked in the laboratory of Professor Peter Lay. Dr Irwin’s doctoral studies focussed on the binding of carcinogenic chromium to saccharides, elucidating solution structures and generating fundamental thermodynamic data for this physiologically relevant system via in vitro EPR spectroscopic studies. Chromium-saccharide complexes were found to damage DNA which is of particular relevance to occupationally induced lung cancers. Her thesis was Sydney University’s nomination for the RACI Cornforth Medal (for best Australian doctoral thesis in the chemical and biological sciences) in 1998. Unfortunately, she was declared in ineligible for the award due to lapsed membership whilst residing in Europe. Over time her interests have focussed on the area of protein chemistry. Fundamental studies of in vitro protein oxidation kinetics in the laboratory of Professor Michael Davies and Professor Roger Dean (Heart Research Institute, Sydney) investigated protein to protein radical transfer under physiologically relevant conditions and the ability of antioxidants to quench these reactions. This research led to more recent forays into proteomics. During her six year tenure with the CSIRO in Canberra she investigated the wheat proteome, and in additional detail the starch biosynthetic pathway, with a view to modifying starch structure in vivo to produce added health benefits. She is currently working in the area of disease biomarker discovery. This work, funded by the CRC for Asthma and Airways, has produced results subject to provisional patenting. She has hands-on experience with all aspects of proteomics including experimental design, sample fractionation, immunodepletion (affinity chromatography), protein separation (2D-DIGE), data analysis, protein identification (mass spectrometry) and biomarker validation. She has considerable expertise in all aspects of biomarker discovery especially in the context of asthma and respiratory diseases.
QualificationsPhD University of Sydney, 1998
B Sc (Hons I) University of Newcastle, 1993
B Sc University of Newcastle, 1985
- Protein Chemistry
- Marker discovery
- Oxidative stress and radical reactions
Refereed Journal Articles
Barr-David, G.; Hambley, T. W.; Irwin, J. A.; Judd. R. J.; Lay, P. A.; Martin, B. D.; Bramley, R.; Dixon, N. E.; Hendry, P.; Ji, J.-Y.; Baker, R. S. U.; Bonin, A. M.; “Suppression by V(IV) of Cr(V)-Mediated DNA Cleavage and Cr(V)/Cr(VI)-Induced Mutagenesis. Synthesis and Crystal Structure of the Vanadium(IV) Complex (NH4)[V(O)(HOC(Et)2COO)(OC(Et)2COO)]”, Inorg. Chem., 1992, 31, 4906-4908. (IF 3.911)
Angus-Dunne, S. J.; Irwin, J. A.; Burns, R. C.; Lawrance, G. A.; Craig, D. C. “Reaction Kinetics and Mechanism of Formation of [MnMo9O32]6- by Hypochlorous Acid Oxidation of Mn2+ in the Presence of Molybdate”. J. Chem. Soc. Dalton Trans., 1993, 18, 2717-2726. (IF 3.01)
Barr-David, G.; Charara, M.; Codd, R.; Farrell, R. P.; Irwin, J. A.; Lay, P. A.; Bramley, R.; Brumby, S.; Ji, J.-Y.; Hanson, G. R. “EPR Characterisation of the CrV Intermediates in the CrVI/V Oxidations of Organic Substrates and of Relevance to Cr-induced Cancers” J. Chem. Soc. Faraday Trans., 1995, 91(8), 1207-1216. (IF 4.73)
Irwin, J. A.; Ostdal, H.; Davies, M. J. “Myoglobin-induced oxidative damage: Evidence for radical transfer from oxidised myoglobin to other proteins and antioxidants” Arch. Biochem. Biophys., 1999, 362, 94-104. (IF 2.969)
Codd R.; Irwin, J. A.; Lay, P. A. “Sialoglycoprotein and carbohydrate complexes in chromium toxicity” Current Opinion in Chemical Biology, 2003, 7, 213-219. (IF 8.32)
Irwin, J. A.; Tolhurst, R.; Jackson, P.; Gale, K. R. “Development of an Enzyme-Linked Immunosorbent Assay for the Detection and Quantification of the Insecticide Tebufenozide in Wine” Food and Agricultural Immunology, 2004, 15(2), 93-104. (IF 0.667)
Nolan, A. L.; Irwin, J. A., Burns, R. C.; Lawrance, G. A. “Reaction kinetics and mechanism of formation of [MnW6O24]8 by hypochlorous acid oxidation of Mn(II)aq in the presence of tungstate” Inorganic Reaction Mechanisms, 2005, 5(3), 181-195. (IF 0.800)
Kosar-Hashemi, B.; Irwin, J. A.; Rahman, S.; Morell, M. K. “Isolation, Characterization and Identification of Starch-interacting Proteins by Two-dimensional Affinity Electrophoresis (2-DAE)” Electrophoresis, 2006, 27(9), 1832-1839. (IF 4.101)
Refereed Conference Proceedings
Baker, R. S. U.; Barr-David, G.; Bonin, A. M.; Dixon, N. E.; Hendry, P.; Farrell, R. P.; Irwin, J. A.; Judd, R. J.; Lay, P. A.; Martin, B. D. “Chemistry, Biochemistry and Mutagenicity of Chromium(V)” J. Inorg. Biochem., 43, 466, presented at the 5th International Conference of Bioinorganic Chemistry, Oxford, U. K., August 4-10, 1991.
Irwin, J. A., Hill, A. S., Jackson, K. L., Webley, D. J. “Antibody Diagnostics for Alternaria alternata Toxins in Wheat and Sorghum” RACI Cereal Chemistry Conference (Gold Coast, 11-14th September, 2000).
Irwin, J. A.; Jackson, P.; Gale, K. R. (2001) “Towards the development of antibody-based diagnostics for selected Alternaria alternata toxins in grain”. Abstract 2.10, p 22. Golden Past, Brilliant Future 51st Australian Cereal Chemistry Conference. Poster.
Irwin, J. A.; Gleeson, M.; Rahman, S; Morell, M.K. (2004) Investigation of the Barley Amyloplast Proteome: a Tool for Better Understanding Cereal Starch Quality. Conference Proceedings of the 54th Australian Cereal Chemistry Conference and 11th Australian Wheat Breeders Assembly, Canberra, ACT, Australia.