2023 |
Alreshidi M, Dunstan H, Roberts T, Alreshidi F, Hossain A, Bardakci F, et al., 'Cytoplasmic amino acid profiles of clinical and ATCC 29213 strains of Staphylococcus aureus harvested at different growth phases', Biomolecules and Biomedicine, 23 1038-1050 (2023) [C1]
Staphylococcus aureus strains are a great contributor to both hospital acquired infections as well as community acquired infections. The objective of the present investigation was... [more]
Staphylococcus aureus strains are a great contributor to both hospital acquired infections as well as community acquired infections. The objective of the present investigation was to compare potential differences in cytoplasmic amino acid levels between clinical and ATCC 29213 strains of S. aureus. The two strains were grown under ideal conditions to mid-exponential and stationary growth phases, after which they were harvested to analyze their amino acid profiles. Initially, the amino acid patterns of both strains were compared at the mid-exponential phase when grown in controlled conditions. At the mid-exponential phase, both strains shared common features in cytoplasmic amino acid levels, with glutamic acid, aspartic acid, proline, and alanine identified as key amino acids. However, the concentration profiles of seven amino acids exhibited major variances between the strains, even though the total cytoplasmic levels of amino acids did not alter significantly. At the stationary phase, the magnitudes of the amino acids abundant in the mid-exponential phase were altered. Aspartic acid became the most abundant amino acid in both strains accounting for 44% and 59% of the total amino acids in the clinical and ATCC 29213 strains, respectively. Lysine was the second most abundant amino acid in both strains, accounting for 16% of the total cytoplasmic amino acids, followed by glutamic acid, the concentration of which was significantly higher in the clinical strain than in the ATCC 29213 strain. Interestingly, histidine was clearly present in the clinical strain but was virtually lacking in the ATCC 29213 strain. This study reveals the dynamic diversity of amino acid levels among strains, which is an essential step toward illustrating the variability in S. aureus cytoplasmic amino acid profiles and could be significant in explaining variances among strains of S. aureus.
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Nova |
2023 |
Alreshidi M, Dunstan H, MacDonald M, Saeed M, Elkahoui S, Roberts T, 'Significant Changes in Cytoplasmic Amino Acid Composition Occur in the Transition between Mid-Exponential and Stationary Phases of Growth of Staphylococcus aureus: An Example of Adaptive Homeostasis in Response to Nutrient Limitations.', Microorganisms, 11 (2023) [C1]
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2022 |
Alreshidi M, Dunstan H, Roberts T, Bardakci F, Badraoui R, Adnan M, et al., 'Changes in Amino Acid Metabolism of Staphylococcus aureus following Growth to the Stationary Phase under Adjusted Growth Conditions', MICROORGANISMS, 10 (2022) [C1]
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Nova |
2022 |
Idrees M, Imran M, Atiq N, Zahra R, Abid R, Alreshidi M, et al., 'Probiotics, their action modality and the use of multi-omics in metamorphosis of commensal microbiota into target-based probiotics', Frontiers in Nutrition, 9 (2022) [C1]
This review article addresses the strategic formulation of human probiotics and allows the reader to walk along the journey that metamorphoses commensal microbiota into target-bas... [more]
This review article addresses the strategic formulation of human probiotics and allows the reader to walk along the journey that metamorphoses commensal microbiota into target-based probiotics. It recapitulates what are probiotics, their history, and the main mechanisms through which probiotics exert beneficial effects on the host. It articulates how a given probiotic preparation could not be all-encompassing and how each probiotic strain has its unique repertoire of functional genes. It answers what criteria should be met to formulate probiotics intended for human use, and why certain probiotics meet ill-fate in pre-clinical and clinical trials? It communicates the reasons that taint the reputation of probiotics and cause discord between the industry, medical and scientific communities. It revisits the notion of host-adapted strains carrying niche-specific genetic modifications. Lastly, this paper emphasizes the strategic development of target-based probiotics using host-adapted microbial isolates with known molecular effectors that would serve as better candidates for bioprophylactic and biotherapeutic interventions in disease-susceptible individuals.
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2020 |
Howley P, Roberts T, 'Engaging school students and educators with the practice of statistics', Statistics Education Research Journal, 19 29-38 (2020) [C1]
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2020 |
Alreshidi MM, Dunstan RH, Macdonald MM, Gottfries J, Roberts TK, 'The Uptake and Release of Amino Acids by Staphylococcus aureus at Mid-Exponential and Stationary Phases and Their Corresponding Responses to Changes in Temperature, pH and Osmolality', Frontiers in Microbiology, 10 (2020) [C1]
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2020 |
Dunstan RH, Macdonald MM, Thorn B, Wood D, Roberts TK, 'Modelling of amino acid turnover in the horse during training and racing: A basis for developing a novel supplementation strategy', PLoS ONE, 15 (2020) [C1]
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Nova |
2020 |
Alreshidi MM, Dunstan RH, Macdonald MM, Singh VK, Roberts TK, 'Analysis of cytoplasmic and secreted proteins of staphylococcus aureus revealed adaptive metabolic homeostasis in response to changes in the environmental conditions representative of the human wound site', Microorganisms, 8 1-22 (2020) [C1]
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2020 |
Thorn B, Dunstan RH, Macdonald MM, Borges N, Roberts TK, 'Evidence that human and equine erythrocytes could have significant roles in the transport and delivery of amino acids to organs and tissues', Amino Acids, 52 711-724 (2020) [C1]
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2019 |
Murphy GR, Hugh Dunstan R, Macdonald MM, Borges N, Radford Z, Sparkes DL, et al., 'Relationships between electrolyte and amino acid compositions in sweat during exercise suggest a role for amino acids and K in reabsorption of Na and Cl from sweat', PLoS ONE, 14 (2019) [C1]
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2019 |
Dunstan RH, Macdonald MM, Marks A, Sparkes DL, Roberts TK, 'Alterations in red blood cell parameters, plasma amino acids, total cholesterol and fatty acids in Standardbred horses undergoing fitness training', COMPARATIVE EXERCISE PHYSIOLOGY, 15 13-23 (2019) [C1]
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2019 |
Alreshidi MM, Dunstan RH, Macdonald MM, Smith ND, Gottfries J, Roberts TK, 'Amino acids and proteomic acclimation of Staphylococcus aureus when incubated in a defined minimal medium supplemented with 5% sodium chloride', MICROBIOLOGYOPEN, 8 (2019) [C1]
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2019 |
Dunstan RH, Macdonald MM, Murphy GR, Thorn B, Roberts TK, 'Modelling of protein turnover provides insight for metabolic demands on those specific amino acids utilised at disproportionately faster rates than other amino acids', Amino Acids, 51 945-959 (2019) [C1]
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Nova |
2018 |
Ho BT, Roberts TK, Lucas S, 'An overview on biodegradation of polystyrene and modified polystyrene: the microbial approach', Critical Reviews in Biotechnology, 38 308-320 (2018) [C1]
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2018 |
Murphy GR, Dunstan RH, Macdonald MM, Gottfries J, Roberts TK, 'Alterations in amino acid metabolism during growth by Staphylococcus aureus following exposure to H2O2 A multifactorial approach', Heliyon, 4 (2018) [C1]
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Nova |
2017 |
Dunstan RH, Sparkes DL, Dascombe BJ, Stevens CJ, Murphy GR, Macdonald MM, et al., 'Sex differences in amino acids lost via sweating could lead to differential susceptibilities to disturbances in nitrogen balance and collagen turnover', AMINO ACIDS, 49 1337-1345 (2017) [C1]
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Nova |
2017 |
Dunstan RH, Sparkes DL, Macdonald MM, De Jonge XJ, Dascombe BJ, Gottfries J, et al., 'Diverse characteristics of the urinary excretion of amino acids in humans and the use of amino acid supplementation to reduce fatigue and sub-health in adults', NUTRITION JOURNAL, 16 (2017) [C1]
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2016 |
Dunstan RH, Sparkes DL, Dascombe BJ, Macdonald MM, Evans CA, Stevens CJ, et al., 'Sweat facilitated amino acid losses in male athletes during exercise at 32-34°C', PLoS ONE, 11 (2016) [C1]
Sweat contains amino acids and electrolytes derived from plasma and athletes can lose 1-2L of sweat per hour during exercise. Sweat may also contain contributions of amino acids a... [more]
Sweat contains amino acids and electrolytes derived from plasma and athletes can lose 1-2L of sweat per hour during exercise. Sweat may also contain contributions of amino acids as well as urea, sodium and potassium from the natural moisturizing factors (NMF) produced in the stratum corneum. In preliminary experiments, one participant was tested on three separate occasions to compare sweat composition with surface water washings from the same area of skin to assess contributions from NMF. Two participants performed a 40 minute self-paced cycle session with sweat collected from cleansed skin at regular intervals to assess the contributions to the sweat load from NMF over the period of exercise. The main study investigated sweat amino acid composition collected from nineteen male athletes following standardised endurance exercise regimes at 32-34°C and 20-30% RH. Plasma was also collected from ten of the athletes to compare sweat and plasma composition of amino acids. The amino acid profiles of the skin washings were similar to the sweat, suggesting that the NMF could contribute certain amino acids into sweat. Since the sweat collected from athletes contained some amino acid contributions from the skin, this fluid was subsequently referred to as "faux" sweat. Samples taken over 40 minutes of exercise showed that these contributions diminished over time and were minimal at 35 minutes. In the main study, the faux sweat samples collected from the athletes with minimal NMF contributions, were characterised by relatively high levels of serine, histidine, ornithine, glycine and alanine compared with the corresponding levels measured in the plasma. Aspartic acid was detected in faux sweat but not in the plasma. Glutamine and proline were lower in the faux sweat than plasma in all the athletes. Three phenotypic groups of athletes were defined based on faux sweat volumes and composition profiles of amino acids with varying relative abundances of histidine, serine, glycine and ornithine. It was concluded that for some individuals, faux sweat resulting from exercise at 32-34°C and 20-30% RH posed a potentially significant source of amino acid loss.
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2016 |
Lloyd-Prichard D, Lucas SA, Roberts T, Haberle S, 'Assessment of Pollen Assemblages from the Hives of Tetragonula Carbonaria for the Presence of the Threatened Species Grevillea Parviflora subsp. Parvaflora', Journal of Pollination Ecology, 18 23-30 (2016) [C1]
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2016 |
Alreshidi MM, Dunstan RH, Gottfries J, Macdonald MM, Crompton MJ, Ang C, et al., 'Changes in the Cytoplasmic Composition of Amino Acids and Proteins Observed in Staphylococcus aureus during Growth under Variable Growth Conditions Representative of the Human Wound Site', PLoS One, 11 (2016) [C1]
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2015 |
Alreshidi MM, Dunstan RH, Macdonald MM, Smith ND, Gottries J, Roberts TK, 'Metabolomic and proteomic responses of Staphylococcus aureus to prolonged cold stress', Journal of Proteomics, 121 44-55 (2015) [C1]
The high pathogenicity of Staphylococcus aureus is thought to be due to its extraordinary capacity to rapidly adapt to changes in environmental conditions. This study was carried ... [more]
The high pathogenicity of Staphylococcus aureus is thought to be due to its extraordinary capacity to rapidly adapt to changes in environmental conditions. This study was carried out to investigate whether the cytoplasmic profiles of metabolites and proteins of S. aureus were altered in response to prolonged exposure to cold stress. Metabolic profiling and proteomics were used to characterise alterations in cytoplasmic proteins and metabolites in cells from the mid-exponential phase of growth under ideal conditions at 37. °C and compared with equivalent cells exposed to prolonged cold stress for 2. weeks at 4. °C. Principle component analysis (PCA) of the metabolomic and proteomic data indicated that, at the mid-exponential phase of growth, prolonged cold stress conditions generated cells with different metabolite and protein profiles compared with those grown at 37. °C. Nine ribosomal proteins and citric acid were substantially elevated in the cytoplasmic fractions from the cells adapted to cold-stress but most amino acids showed a reduction in their concentration in cold-stressed samples. The data provided strong evidence supporting the hypothesis that specific changes in metabolic homeostasis and protein composition were critical to the adaptive processes required for survival under cold stress. Biological significance: Work in our laboratory has shown that prolonged exposure of S. aureus to cold stress can result in the formation of small colony variants (SCVs) associated with significant alterations in the cell wall composition [8]. Further studies revealed that S. aureus altered cell size and cell wall thickness in response to exposure to cold temperatures, alterations in pH and exposure to antibiotics [10]. The current study has utilised the prolonged exposure to cold stress as a model system to explore changes in the proteome and associated metabolic homeostasis following environmental challenges. The study provides an improved understanding of how S. aureus adapts to the changing environment whilst in transition between human hosts. The results indicated an unexpected production of 9 ribosomal proteins and citric acid in response to cold stress suggesting specific survival roles for these proteins and citric acid as an adaptation mechanism for empowering survival under these conditions.
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2015 |
Thanh Ba Ho, Timothy Kilgour Roberts, Steven Lucas, 'Small-Scale Household Biogas Digesters as a Viable Option for Energy Recovery and Global Warming Mitigation Vietnam Case Study', Journal of Agricultural Science and Technology A, 5 (2015)
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2015 |
Dunstan RH, Sparkes DL, Dascombe BJ, Evans CA, Macdonald MM, Crompton M, et al., 'Sweat facilitated losses of amino acids in Standardbred horses and the application of supplementation strategies to maintain condition during training', Comparative Exercise Physiology, 11 201-212 (2015) [C1]
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2014 |
Crompton MJ, Dunstan RH, Macdonald MM, Gottfries J, Von Eiff C, Roberts TK, 'Small changes in environmental parameters lead to alterations in antibiotic resistance, cell morphology and membrane fatty acid composition in Staphylococcus lugdunensis', PLoS ONE, 9 (2014) [C1]
Staphylococcus lugdunensis has emerged as a major cause of community-acquired and nosocomial infections. This bacterium can rapidly adapt to changing environmental conditions to s... [more]
Staphylococcus lugdunensis has emerged as a major cause of community-acquired and nosocomial infections. This bacterium can rapidly adapt to changing environmental conditions to survive and capitalize on opportunities to colonize and infect through wound surfaces. It was proposed that S. lugdunensis would have underlying alterations in metabolic homeostasis to provide the necessary levels of adaptive protection. The aims of this project were to examine the impacts of subtle variations in environmental conditions on growth characteristics, cell size and membrane fatty acid composition in S. lugdunensis. Liquid broth cultures of S. lugdunensis were grown under varying combinations of pH (6-8), temperature (35-39°C) and osmotic pressure (0-5% sodium chloride w/w) to reflect potential ranges of conditions encountered during transition from skin surfaces to invasion of wound sites. The cells were harvested at the mid-exponential phase of growth and assessed for antibiotic minimal inhibitory concentration (MIC), generation time, formation of small colony variants, cell size (by scanning electron microscopy) and membrane fatty acid composition. Stress regimes with elevated NaCl concentrations resulted in significantly higher antibiotic resistance (MIC) and three of the combinations with 5% NaCl had increased generation times (P<0.05). It was found that all ten experimental growth regimes, including the control and centroid cultures, yielded significantly different profiles of plasma membrane fatty acid composition (P<0.0001). Alterations in cell size (P<0.01) were also observed under the range of conditions with the most substantial reduction occurring when cells were grown at 39°C, pH 8 (514±52 nm, mean ± Standard Deviation) compared with cells grown under control conditions at 37°C with pH 7 (702±76 nm, P<0.01). It was concluded that S. lugdunensis responded to slight changes in environmental conditions by altering plasma membrane fatty acid composition, growth rates and morphology to achieve optimal adaptations for survival in changing environments. © 2014 Crompton et al.
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2014 |
Mayne P, Song S, Shao R, Burke J, Wang Y, Roberts T, 'Evidence for Ixodes holocyclus (Acarina: Ixodidae) as a Vector for Human Lyme Borreliosis Infection in Australia', JOURNAL OF INSECT SCIENCE, 14 (2014)
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2014 |
Dunstan RH, Sparkes DL, Roberts TK, Dascombe BJ, 'Preliminary Evaluations of a Complex Amino Acid Supplement, Fatigue Reviva, to Reduce Fatigue in a Group of Professional Male Athletes and a Group of Males Recruited from the General Public', Food and Nutrition Sciences, 5 231-235 (2014) [C1]
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Nova |
2013 |
Crowley ET, Williams LT, Roberts TK, Dunstan RH, Jones PD, 'Does Milk Cause Constipation? A Crossover Dietary Trial', NUTRIENTS, 5 253-266 (2013) [C1]
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Nova |
2013 |
Dunstan RH, Sparkes DL, Roberts TK, Crompton MJ, Gottfries J, Dascombe BJ, 'Development of a complex amino acid supplement, Fatigue Reviva (TM), for oral ingestion: initial evaluations of product concept and impact on symptoms of sub-health in a group of males', NUTRITION JOURNAL, 12 (2013) [C1]
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Nova |
2013 |
Onyango LA, Dunstan RH, Roberts TK, Macdonald MM, Gottfries J, 'Phenotypic Variants of Staphylococci and Their Underlying Population Distributions Following Exposure to Stress', PLOS ONE, 8 (2013) [C1]
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Nova |
2012 |
Onyango LSA, Dunstan RH, Gottfries J, Von Eiff C, Roberts TK, 'Effect of low temperature on growth and ultra-structure of Staphylococcus spp', PLoS One, 7 (2012) [C1]
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Nova |
2012 |
Dunstan RH, Sparkes DL, Wratten C, Denham JW, Gottfries J, Roberts TK, MacDonald MM, 'Metabolic, health and lifestyle profiling of breast cancer radiotherapy patients and the risk of developing fatigue', Journal of Cancer Therapy, 3 731-740 (2012) [C1]
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Nova |
2011 |
Dunstan RH, Ho P-H, Adams MC, Rothkirch TB, Roberts TK, 'Opioid peptide digestion by newly isolated potential probiotic bacteria from foods', Journal of Science and Technology, 49 161-168 (2011)
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2011 |
Dunstan RH, Sparkes DL, MacDonald MM, Roberts TK, Wratten C, Kumar M, et al., 'Altered amino acid homeostasis and the development of fatigue by breast cancer radiotherapy patients: A pilot study', Clinical Biochemistry, 44 208-215 (2011) [C1]
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Nova |
2010 |
Onyango LA, Dunstan RH, Roberts TK, 'Filterability of staphylococcal species through membrane filters following application of stressors', BMC Research Notes, 3 (2010) [C2]
Background. Passage of bacterial cells through filter pores has been reported for a number of bacterial species. In this investigation, we tested the filterability of staphylococc... [more]
Background. Passage of bacterial cells through filter pores has been reported for a number of bacterial species. In this investigation, we tested the filterability of staphylococcal cultures that were exposed to several environmental stress conditions by passing them through 0.22 and 0.45 m sterile filters, which are industry standards. Findings. Results showed repeated passage of viable staphylococcal cells through both pore sizes, although more passage was seen through the 0.45 m pore size. Of the three staphylococcal species, S. lugdunensis showed the best passage at relatively higher numbers regardless of the treatment, while both S. aureus and S. epidermidis showed limited passage or complete inhibition. Conclusion. The data showed that staphylococcal bacteria were capable of passing through sterile filters in a viable state. There was better passage through 0.45 m sterile filters than through the 0.22 m sterile filters. Application of a stress condition did not appear to enhance filterability of these bacterial cultures. © 2010 Roberts et al; licensee BioMed Central Ltd.
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2008 |
Evans CA, Dunstan RH, Rothkirch TB, Roberts TK, Reichelt KL, Cosford RE, et al., 'Altered amino acid excretion in children with autism', Nutritional Neuroscience, 11 9-17 (2008) [C1]
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Nova |
2008 |
Onyango LSA, Dunstan RH, Roberts TK, 'Small colony variants of staphylococci: Pathogenesis and evolutionary significance in causing and sustaining problematic human infections', Journal of Nutritional & Environmental Medicine, 17 56-75 (2008) [C1]
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Nova |
2008 |
Crowley ET, Williams LT, Roberts TK, Jones PD, Dunstan RH, 'Evidence for a role of cow's milk consumption in chronic functional constipation in children: Systematic review of the literature from 1980 to 2006', Nutrition & Dietetics, 65 29-35 (2008) [C1]
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Nova |
2008 |
Shah VG, Dunstan RH, Geary PM, Coombes P, Roberts T, Von Nagy-Felsobuki E, 'Evaluating potential applications of faecal sterols in distinguishing sources of faecal contamination from mixed faecal samples (vol 41, pg 16, 2007)', WATER RESEARCH, 42 1324-1324 (2008) [C3]
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2007 |
Shah V, Dunstan RH, Geary PM, Coombes PJ, Roberts TK, Rothkirch TB, 'Comparisons of water quality parameters from diverse catchments during dry periods and following rain events', Water Research, 41 3655-3666 (2007) [C1]
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2007 |
Niblett SH, King KE, Dunstan RH, Clifton-Bligh P, Hoskin LA, Roberts TK, et al., 'Hematologic and urinary excretion anomalies in patients with chronic fatigue syndrome', Experimental Biology and Medicine, 232 1041-1049 (2007) [C1]
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2007 |
Shah V, Dunstan RH, Geary PM, Coombes PJ, Roberts TK, Von Nagy-Felsobuki EI, 'Evaluating potential applications of faecal sterols in distinguishing sources of faecal contamination from mixed faecal samples', Water Research, 41 3691-3700 (2007) [C1]
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2007 |
Shah V, Dunstan RH, Geary PM, Coombes PJ, Roberts TK, Rothkirch TB, 'Bacterial source tracking from diverse land use catchments by sterol ratios', Water Research, 41 3667-3674 (2007) [C1]
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2007 |
Morrow AC, Dunstan RH, King BV, Roberts TK, 'Metabolic effects of static magnetic fields on Streptococcus Pyogenes', Bioelectromagnetics, 28 439-445 (2007) [C1]
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2006 |
Martin A, Dunstan RH, Roberts TK, Brown GK, 'Babesia canis vogeli: A novel PCR for its detection in dogs in Australia (Short communication)', Experimental Parasitology, 112 63-65 (2006) [C1]
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2006 |
Omwandho CA, Gruessner SE, Falconer J, Mala GO, Mecha EO, Tumbo-Oeri AG, et al., 'Ovine placental eluate immunoglobulins recognise isologous and third party acid-treated trophoblast microvesicle antigens in vitro', Scientific Journal of the South African Veterinary Association, 77 24-27 (2006) [C1] |
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2006 |
Brown GK, Canfield PJ, Dunstan RH, Roberts TK, Martin A, Brown CS, Irving R, 'Detection of Anaplasma platys and Babesia canis vogeli and their impact on platelet numbers in free-roaming dogs associated with remote Aboriginal communities in Australia', Australian Veterinary Journal, 84 321-325 (2006) [C1]
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2005 |
Brown GK, Martin A, Roberts TK, Dunstan RH, 'Molecular detection of Anaplasma platys in lice collected from dogs in Australia', Australian Veterinary Journal, 83 101-102 (2005) [C3]
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Nova |
2005 |
Omwandho CA, Mecha E, Tumbo-Oeri AG, Falconer J, Gruessner SE, Tinneberg H-R, Roberts TK, 'Human placental immunoglobulins show unique re-association patterns with isologous and third party acid treated trophoblast microvesicles in vitro', East African Medical Journal, 82 290-293 (2005) [C1]
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2005 |
Omwandho CA, Gruessner SE, Falconer J, Mecha E, Tumbo-Oeri AG, Tinneberg HR, Roberts TK, 'Immunoglobulin G bound to ovine placenta is eluted by surgical cannulation and acid perfusion in situ', East African Medical Journal, 82 468-472 (2005)
Objective: To elute placental bound immunoglobulin G (IgG) in situ. Design: Laboratory based experimentation. Setting: Biological Sciences Department, The University of Newcastle ... [more]
Objective: To elute placental bound immunoglobulin G (IgG) in situ. Design: Laboratory based experimentation. Setting: Biological Sciences Department, The University of Newcastle Australia and the Department of Biochemistry, University of Nairobi, Kenya. Subjects: Twelve pregnant ewes 10 to 15 days before the onset of natural parturition. Results: Placental eluates were rich in IgG 1 and IgG2. The relative molecular weigh of placental IgG was estimated at 158kDa by gel filtration chromatography. Analysis of eluate by SDS PAGE revealed the heavy and light chains of IgG at 57 and 27kDa respectively together giving a relative molecular weight of 168kDa. Conclusion: Placental bound IgG may be crucial in immunology of pregnancy and together with the cognate antigen thereof may be useful as models for the study of maternal-fetal interaction in human pregnancy and in the development of experimental immunotherapy to immunologically compromised pregnancies in humans and livestock.
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2005 |
Richards RS, McGregor NR, Roberts TK, 'Association Between Oxidative Damage Markers and Self-Reported Temporomandibular Dysfunction Symptoms in Patients with Chronic Fatigue Syndrome', Journal of Chronic Fatigue Syndrome, 12 45-61 (2005) [C1]
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Nova |
2005 |
Metcalf LN, McGregor NR, Roberts TK, 'Membrane Damaging Toxins from Coagulase-Negative Staphylococcus Are Associated with Self-Reported Temporomandibular Disorder (TMD) in Patients with Chronic Fatigue Syndrome', Journal of Chronic Fatigue Syndrome, 12 25-43 (2005) [C1]
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Nova |
2005 |
Martin A, Brown GK, Dunstan RH, Roberts TK, 'Anaplasma platys: an improved PCR for its detection in dogs', Experimental Parasitology, 109 176-180 (2005) [C1]
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Nova |
2004 |
Gifford SP, Dunstan RH, O'Connor W, Roberts TK, Toia RF, 'Pearl aquaculture-profitable environmental remediation?', Science of the Total Environment, 319 27-37 (2004) [C1]
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2004 |
Omwandho COA, Greussner SEM, Roberts TK, Tinneberg HR, 'Intravenous immunoglobulin (IVIG): modes of action in the clinical management of recurrent pregnancy loss (RPL) and selected autoimmune disorders', Clinical Chemistry and Laboratory Medicine, 42 359-370 (2004) [C1]
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2003 |
Sparkes DL, Robinson GL, Dunstan RH, Roberts TK, 'Plasma Cholesterol Levels And Irlen Syndrome: Preliminary Study of 10- to 17-yr-old Students', Perceptual And Motor Skills, Vol 97 743-752 (2003) [C1]
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2003 |
Roberts TK, Brown GK, Martin A, Dunstan RH, 'Going to the dogs: ticks and emerging diseases', Today's Life Science, 15 32-34 (2003) [C1] |
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2003 |
McGregor N, Zerbes M, Niblett SH, Dunstan RH, Roberts TK, Butt HL, Klineberg IJ, 'Coagulase-Negative Staphylococcol Membrane-Damaging Toxins, Pain Intensity, and Metabolic Changes in Temporomandibular Disorder Patients with Chronic Muscle Pain', Journal of Orofacial Pain, 17 125-132 (2003) [C1]
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Nova |
2003 |
McGregor N, Zerbes M, Niblett SH, Dunstan RH, Roberts TK, Butt HL, Klineberg IJ, 'Pain Intensity, Illness Duration, and Protein Catabolism in Temporomandibular Disorder Patients with Chronic Muscle Pain', Journal of Orofacial Pain, 17 112-124 (2003) [C1]
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2001 |
Brown GK, Martin A, Roberts TK, Aitken RJ, 'Detection of Ehrlichia platys in dogs in Australia', Australian Veterinary Journal, 79 23-27 (2001) [C1]
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Nova |
2001 |
Robinson GL, McGregor NR, Roberts TK, Dunstan RH, Butt H, 'A Biochemical analysis of people with chronic fatigue who have irlen syndrome: Speculation concerning immune system dysfunction', Perceptual and Motor Skills, 93 486-504 (2001) [C1]
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2000 |
Omwandho CA, Tinneberg HR, Tumbo-Oeri AG, Roberts TK, Falconer J, 'Recurrent pregnancy losses and the role of immunotherapy', ARCHIVES OF GYNECOLOGY AND OBSTETRICS, 264, ISSUE 1 3-12 (2000) [C1]
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2000 |
Richards RS, Roberts TK, Mathers D, Dunstan RH, McGregor NR, Butt HL, 'Erythrocyte morphology in rheumatoid arthritis and chronic fatigue syndrome. A preliminary study', JOURNAL OF CHRONIC FATIGUE SYNDROME, 6, NO. 1 23-35 (2000) [C1]
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2000 |
Richards RS, Roberts TK, Mathers D, Dunstan RH, McGregor NR, Butt HL, 'Investigation of erythrocyte oxidative damage in rheumatoid arthritis in chronic fatigue syndrome', JOURNAL OF CHRONIC FATIGUE SYNDROME, 6, NO. 1 37-46 (2000) [C1]
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2000 |
McGregor NR, Niblett SH, Bligh PC, Dunstan RH, Fulcher G, Hoskin L, et al., 'The biochemistry of chronic pain and fatigue', JOURNAL OF CHRONIC FATIGUE SYNDROME, 7, NO.1 3-21 (2000) [C1]
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2000 |
Dunstan RH, McGregor NR, Roberts TK, Butt HL, Niblett SH, Rothkirch TB, 'The development of laboratory-based tests inchronic pain fatigue. 1. Muscle catabolism and coagulase negative staphylococci which produce membrane damaging toxins', JOURNAL OF CHRONIC FATIGUE SYNDROME, 7, NO. 2 53-57 (2000) [C1]
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2000 |
Dunstan RH, McGregor NR, Roberts TK, Butt HL, Taylor WG, Carter AC, 'The development of laboratory-based tests in chronic pain and fatigue: 2. Essential fatty acids and cholesterol', JOURNAL OF CHRONIC FATIGUE SYNDROME, 7, NO. 2 59-62 (2000) [C1] |
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2000 |
Dunstan RH, McGregor NR, Roberts TK, Butt H, Niblett S, Rothkirch T, 'The development of laboratory-based tests in chronic pain and fatigue: 1. Muscle catabolism and coagulase negative staphylococci which produce membrane damaging toxins', Journal of Chronic Fatigue Syndrome, 7 23-27 (2000)
Background: The diagnosis of chronic fatigue syndrome (CFS) requires the exclusion of other known fatigue-related diseases because the core symptoms of CFS represent a general hos... [more]
Background: The diagnosis of chronic fatigue syndrome (CFS) requires the exclusion of other known fatigue-related diseases because the core symptoms of CFS represent a general host response to many well-defined diseases. The patient set derived by this process is heterogeneous in their polysymptomatic presentation and has proved very difficult to study clinically and scientifically. Objectives: To investigate the alterations in urine excretion and microbiology in patients with CFS. Results: CFS patients had multiple anomalies in their amino and organic acid homeostasis. Sub-groups of CFS patients could be delineated on the basis of their urine excretion and their symptom presentation. The most common feature was an active muscle catabolism resulting in a depletion of amino acids and associated organic and keto-acids. The extent of muscle catabolism was directly correlated to pain severity. The carriage of toxin-producing coagulase negative staphylococci (MDT-CoNS) was strongly correlated with the catabolic response and pare severity. Conclusions: An hypothesis has been constructed where an occult pathogen, such as MDT-CoNS, may be an aetiological agent contributing to the sustenance of a chronic fatigue/pain disorder, a comorbid pathogen. Urine analysis offers an opportunity for assessment of muscle catabolism and sub-classification of chronic fatigue patients leading to a number of management options. The detection of MDT-CoNS identifies potentially treatable agents that contribute to the fatigue and pain condition.
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2000 |
Richards RS, Roberts TK, McGregor NR, Dunstan RH, Butt HL, 'Blood parameters indicative of oxidative stress are associated with symptom expression in chronic fatigue syndrome', REDOX REPORT, 5, NO. 1 35-41 (2000) [C1]
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2000 |
Richards RS, Roberts TK, Dunstan RH, McGregor NR, Butt HL, 'Free radicals in chronic fatigue syndrome: cause or effect?', Redox Report, 5 146-147 (2000) [C2]
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2000 |
McGregor NR, Dunstan RH, Donohoe M, Roberts TK, Butt HL, Watkins JA, et al., 'Assessment of plasma fatty acids and sterols in sudden- and gradual-onset chronic fatigue syndrome patients', JOURNAL OF NUTRITIONAL & ENVIRONMENTAL MEDICINE, 10 13-23 (2000) [C1]
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2000 |
Dunstan RH, McGregor NR, Butt HL, Roberts TK, Klineberg IJ, Niblett SH, et al., 'Characterization of differential amino acid homeostasis amongst population subgroups: A basis for determining specific amino acid requirements', JOURNAL OF NUTRITIONAL & ENVIRONMENTAL MEDICINE, 10 211-223 (2000) [C1]
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1999 |
Robinson GL, Roberts TK, McGregor NR, Dunstan RH, Butt H, 'Understanding the causal mechanisms of visual processing problems - A possible biochemical basis for Irlen Syndrome?', Australian Journal of Learning Disabilities, 4 (4) 21-29 (1999) [C1] |
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1999 |
Mburu DN, Roberts TK, Boettcher B, 'Overexpression of human testis antigens in Escherichia coli host cells is influenced by site of expression and the induction temperature', BIOCHEMISTRY AND MOLECULAR BIOLOGY INTERNATIONAL, 47 1009-1018 (1999) |
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1999 |
Dunstan RH, McGregor NR, Butt HL, Roberts TK, 'Biochemical and microbiological anomalies in chronic fatigue syndrome: the development of laboratory based tests and the possible role of toxic chemicals', Journal of Nutritional & Environmental Medicine, 9 97-108 (1999) [C1]
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1999 |
Dunstan RH, McGregor NR, Watkins JA, Donohoe M, Roberts TK, Butt HL, et al., 'Changes in plasma lipid homeostasis observed in chromic fatigue syndrome patients', Journal of Nutritional & Environmental Medicine, 9 267-279 (1999) [C1]
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1998 |
Klineberg I, McGregor N, Butt HL, Dunstan RH, Roberts TK, Zerbes M, 'Chronic Orofacial Muscle Pain: A new approach to diagnosis and management', Alpha Omegan, 91 25-28 (1998) [C3]
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1998 |
Richards RS, Roberts TK, Dunstan RH, McGregor NR, Butt HL, 'Erythrocyte antioxidant systems protect cultured endothelial cells against oxidant damage', Biochemistry and Molecular Biology International, 46 857-865 (1998) [C1]
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1998 |
Butt HL, Dunstan RH, McGregor NR, Roberts TK, Zerbes M, Klineberg IJ, 'An association of membrane-damaging toxins from coagulase-negative staphylococci and chronic orofacial muscle pain', J. Med. Microbiol, 47 577-584 (1998) [C1]
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1998 |
Roberts TK, McGregor NR, Dunstan RH, Donohoe M, Murdoch RN, Hope D, et al., 'Immunological and haematological parameters in patients with chronic fatigue syndrome', Journal of Chronic Fatigue Syndrome, 4 51-65 (1998) [C1]
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1998 |
Richards RS, Roberts TK, McGregor NR, Dunstan RH, Butt HL, 'The role of erythrocytes in the inactivation of free radicals', Medical Hypotheses, 50 363-367 (1998) [C1]
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1997 |
McGregor NR, Dunstan RH, Butt HL, Roberts TK, Klineberg IJ, Zerbes M, 'A preliminary assessment of the association of SCL-90-R psychological inventory responses with changes in urinary metabolites in patients with chronic fatigue syndrome', Journal of Chronic Fatigue Syndrome, 3 17-37 (1997)
A previous investigation of a cohort of 20 chronic fatigue syndrome (CFS) patients revealed an increased urinary excretion of an unusual metabolite, tentatively identified as amin... [more]
A previous investigation of a cohort of 20 chronic fatigue syndrome (CFS) patients revealed an increased urinary excretion of an unusual metabolite, tentatively identified as amino-hydroxy-N-methyl-pyrrolidine (coded CFSUM1) and ß-alanine, compared with 45 control subjects. The relative abundances of both CFSUM1 and ß-alanine were positively associated with the core diagnostic symptoms of CFS and associated changes in amino and organic acid excretion. The psychological attributes of these CFS patients and controls were assessed in this study by using the Symptom Check List-90- revised (SCL-90-R) psychological inventory. The CFS patients had increases in the SCL-90-R somatization, obsessive compulsive, depression, anxiety and phobic anxiety dimension scores. Nineteen of 20 CFS patients had somatization T-scores = 63 (P < 0.0001), suggestive of a somatization disorder. Multiple regression analysis indicated that somatization was the most important SCL- 90-R-defined dimension discriminating CFS from control subjects. Depression and anxiety were not found to be important inter-group determinants. The dimension scores were each related to specific changes in the urinary excretion of organic and amino acids, suggesting that each is biochemically distinct and has an organic basis. Cluster analysis of dimension profiles revealed that the profile with increased prevalence (P < 0.0001) in CFS patients was associated with increased excretion of CFSUM1 (P < 0.005) and had increases in somatization, obsessive compulsion and depression dimension scores. The PSDI as a measure of SCL-90-R symptom severity was positively correlated with CFSUM1 (model P < 0.003). CFSUM1 was also the primary correlate for the somatization dimension (model P < 0.0008), but was not associated with any other SCL-90-R-defined dimension. Another unidentified urinary metabolite, coded UM15, was the primary correlate for depression (model P < 0.0004) and was associated with multiple dimension elevations by both cluster and logistic regression analysis; the excretion of this compound was unrelated to CFSUM1. These results indicated that, in this CFS cohort, the SCL-90-R defined psychological changes were strongly associated with changes in the biochemical homeostasis of patients, suggestive of an organic basis to CFS.
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1997 |
Butt H, Mcgregor N, Au A, Wong E, Harrison T, Dunstan H, et al., 'Staphylococcal species and orofacial pain patient management.', JOURNAL OF DENTAL RESEARCH, 76 936-936 (1997) |
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1997 |
Au A, Butt H, Mcgregor N, Wong E, Harrison T, Dunstan H, et al., 'Carriage of Staphylococci and urinary organic and amino acids.', JOURNAL OF DENTAL RESEARCH, 76 936-936 (1997) |
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1997 |
Wong EW, Au AR, McGregor NR, Dunstan RH, Taylor W, Roberts T, et al., 'Preliminary assessment of urinary profiles of chronic orofacial pain patients.', JOURNAL OF DENTAL RESEARCH, 76 939-939 (1997) |
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1997 |
Holmgreen SP, Wang X, Clarke GR, Noltorp RS, Roberts TK, Burton RC, et al., 'Phosphorylation of the NC-1.1 receptor and regulation of natural cytotoxicity by protein kinase C and cyclic GMP-Dependent protein kinase', JOURNAL OF IMMUNOLOGY, 158 2035-2041 (1997)
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1997 |
Brown R, Andren J, Andrews K, Brown L, Chidgey J, Geary N, et al., 'Erratum: Muramyl peptides and the functions of sleep (Behavioural Brain Research 69 (1995) 85-90)', Behavioural Brain Research, 82 245 (1997)
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1997 |
Omwandho CA, Hall AL, Falconer J, Roberts TK, 'Elution and partial characterization of immunoglobulins bound to ovine placenta', IMMUNOLOGY AND CELL BIOLOGY, 75 231-237 (1997)
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1997 |
Richards RS, Roberts TK, McGregor NR, Dunstan RH, Butt HL, 'Erythrocytes protect cultured endothelial cells from neutrophil-mediated damage', Australian Journal of Medical Science, 18 73-77 (1997)
A study was undertaken to assess the ability of the erythrocyte to provide protection to other tissues against oxidative damage, Radiolabelled (51Cr) human umbilical vein endothel... [more]
A study was undertaken to assess the ability of the erythrocyte to provide protection to other tissues against oxidative damage, Radiolabelled (51Cr) human umbilical vein endothelial cells (HUVEC) were incubated with erythrocytes and neutrophils activated with phorbol myristate acetate (PMA). Damage to the endothelial cells was indicated by release of radioactivity into the suspending medium. We found that the co-incubation of HUVEC with an increasing range of erythrocyte concentrations resulted in a dose dependent reduction in the release of radioactivity. These data suggest that the erythrocytes afforded some protection against endothelial cell damage by the activated neutrophils. When PMA-activated neutrophils were incubated with erythrocytes, the erythrocytes had reduced levels of 2,3-diphosphoglycerate (p < 0.01) and glutathione (p < 0.01) compared with erythrocytes incubated alone, indicating utilisation of antioxidant potential. The same erythrocytes had increased concentrations of the oxidative damage products, malondialdehyde (p < 0.02) and methaemoglobin (p < 0.002). These data support the hypothesis that erythrocytes can provide antioxidant protection to other tissues.
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1997 |
Richards RS, Roberts TK, Dunstan RH, McGregor NR, Butt HL, 'Erythrocytes as scavengers of free radicals', Australian Journal of Medical Science, 18 142 (1997)
We have recently proposed that the eiythran acts as a major deactivate! of reactive free radical species in vivo in addition to its recognised role of gas transport (1). The expen... [more]
We have recently proposed that the eiythran acts as a major deactivate! of reactive free radical species in vivo in addition to its recognised role of gas transport (1). The expendable nature of the individual erythrocyte would be a desirable attribute for such a detoxification unit As a consequence of this function, the labile components of individual erythrocytes would suffer oxidative damage over a period of time leading to biochemical and structural changes. The alterations, including those to membrane integrity, would be reflected by changes in shape and function. To test this hypothesis, radiolabelled ("Cor) human umbilical vein endothelial cells (HUVEQ, were incubated with neutrophils activated with phorbol myristate acetate (PMA) and red cells. Damage to the endothelial cells, indicated by release of radioactivity into the suspending medium, was proportionately reduced by increasing concentrations of erythrocytes. Erythrocytes, modified so that Superoxide was unable to cross the membrane or so that the glutathione or catalase mechanism was inactive, were less efficient at preventing release of radioactivity. Erythrocytes incubated with PMA activated neutrophils contained less 2,3-diphosphoglycerate (2,3-DPG) (p=0.01), less glutathione (p=0.01), more malondialdehyde (p=0.02) and more methaemoglobin (p=0.002) than those incubated alone or with unactivated neutrophils. The depleted 2,3-DPG and glutathione are presumably the result of the acute oxidative stress in this environment We have found increased levels of these metabolites in erythrocytes from chronically stressed environments such as rheumatoid arthritis or renal dialysis presumably to compensate for the increased demand. These findings demonstrate that erythrocytes undergo biochemical changes in environments of high oxidative stress and support our hypothesis that they behave as free radical scavengers under certain conditions.
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1996 |
Dunstan RH, Donohoe M, Taylor W, Roberts TK, Murdoch RN, Watkins JA, McGregor NR, 'Chlorinated hydrocarbons and chronic fatigue syndrome', MEDICAL JOURNAL OF AUSTRALIA, 164 251-251 (1996)
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1996 |
McGregor NR, Butt HL, Zerbes M, Klineberg IJ, Dunstan RH, Roberts TK, 'Assessment of pain (distribution and onset), symptoms, SCL-90-R inventory responses, and the association with infectious events in patients with chronic orofacial pain', Journal of Orofacial Pain, 10 339-350 (1996)
A visual analog pain scale and scalar responses to 13 pain/symptom indicator Symptom Checklist-90-Revised (SCL-90-R) questions were used to assess symptom prevalence and pain seve... [more]
A visual analog pain scale and scalar responses to 13 pain/symptom indicator Symptom Checklist-90-Revised (SCL-90-R) questions were used to assess symptom prevalence and pain severity in 43 chronic orofacial muscle pain patients and 40 control subjects. The orofacial muscle pain group reported pain in an axial skeletal distribution; neurocognitive, gastrogenitourinary, and musculoskeletal symptoms; infectious events at or preceding onset; similar symptoms in sexual partners; and low prevalence of trauma. Sudden onset was reported by 30.2% of pain patients. Strong associations were found between chronic orofacial muscle pain and (1) onset-related infectiouslike events (67.4%); (2) a higher prevalence of history of respiratory and gastrogenitourinary infectious events; and (3) high prevalences of similar pain symptoms in long-term sexual partners. The SCL-90-R somatization scores (> 62) had a higher prevalence in the chronic pain group. No prevalence differences or associations with pain/symptom indicators were found for depression or anxiety dimension scores. These data suggest that patients with recurrent systemic infectious events have a higher prevalence of reporting of chronic orofacial muscle pain compared with control subjects, and these infectious events are associated with the onset of chronic orofacial muscle pain in 67% of patients.
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1996 |
McGregor NR, Dunstan RH, Zerbes M, Butt HL, Roberts TK, Klineberg IJ, 'Preliminary determination of a molecular basis to chronic fatigue syndrome', BIOCHEMICAL AND MOLECULAR MEDICINE, 57 73-80 (1996)
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1996 |
Dunstan RH, Roberts TK, Donohoe M, McGregor NR, Hope D, Taylor WG, et al., 'Bioaccumulated chlorinated hydrocarbons and red/white blood cell parameters', BIOCHEMICAL AND MOLECULAR MEDICINE, 58 77-84 (1996)
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1996 |
McGregor NR, Dunstan RH, Zerbes M, Butt HL, Roberts TK, Klineberg IJ, 'Preliminary determination of the association between symptom expression and urinary metabolites in subjects with chronic fatigue syndrome', BIOCHEMICAL AND MOLECULAR MEDICINE, 58 85-92 (1996)
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1995 |
DUNSTAN RH, DONOHOE M, TAYLOR W, ROBERTS TK, MURDOCH RN, WATKINS JA, MCGREGOR NR, 'A PRELIMINARY INVESTIGATION OF CHLORINATED HYDROCARBONS AND CHRONIC FATIGUE SYNDROME', MEDICAL JOURNAL OF AUSTRALIA, 163 294-297 (1995)
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1994 |
WALSH K, DUNSTAN RH, MURDOCH RN, CONROY BA, ROBERTS TK, LAKE P, 'BIOACCUMULATION OF POLLUTANTS AND CHANGES IN POPULATION PARAMETERS IN THE GASTROPOD MOLLUSK AUSTROCOCHLEA-CONSTRICTA', ARCHIVES OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY, 26 367-373 (1994)
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1994 |
CLARKE GR, ROBERTS TK, SMART YC, 'NATURAL-KILLER AND NATURAL CYTOTOXIC-CELLS ARE PRESENT AT THE MATERNAL-FETAL INTERFACE DURING MURINE PREGNANCY', IMMUNOLOGY AND CELL BIOLOGY, 72 153-160 (1994)
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1992 |
GRAY AB, SMART YC, TELFORD RD, WEIDEMANN MJ, ROBERTS TK, 'ANAEROBIC EXERCISE CAUSES TRANSIENT CHANGES IN LEUKOCYTE SUBSETS AND IL-2R EXPRESSION', MEDICINE AND SCIENCE IN SPORTS AND EXERCISE, 24 1332-1338 (1992)
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1992 |
PODSIADLY BT, ADAMSON LM, STANGER JD, SMART YC, ROBERTS TK, 'PREFERTILIZATION TREATMENT OF MICE WITH PLATELET-ACTIVATING-FACTOR AFFECTS PREGNANCY', MOLECULAR REPRODUCTION AND DEVELOPMENT, 32 363-368 (1992)
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1991 |
HANF V, OETTLING G, ROBERTS TK, TINNEBERG HR, 'EFFECTS OF EPF TREATMENT IN HUMAN MONONUCLEAR-CELLS', JOURNAL OF REPRODUCTIVE IMMUNOLOGY, 20 175-182 (1991)
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1991 |
ADAMSON LM, PODSIADLY B, SMART YC, STANGER JD, ROBERTS TK, 'STUDIES ON MURINE EMBRYO-DERIVED PLATELET-ACTIVATING-FACTOR (EPAF)', MOLECULAR REPRODUCTION AND DEVELOPMENT, 30 207-213 (1991)
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1989 |
SHAFREN DR, TANNOCK GA, ROBERTS TK, 'DEVELOPMENT AND APPLICATION OF AN ELISA TECHNIQUE FOR THE DETECTION OF ANTIBODY TO AVIAN ENCEPHALOMYELITIS VIRUSES', RESEARCH IN VETERINARY SCIENCE, 46 95-99 (1989)
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1988 |
ALLEN RD, ROBERTS TK, 'THE ROLE OF SPERMINE OXIDATION IN SEMINAL PLASMA IMMUNOSUPPRESSION INVITRO', AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY AND MICROBIOLOGY, 16 106-106 (1988) |
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1988 |
ALLEN RD, ROBERTS TK, 'IMMUNOMODULATION BY SEMINAL PLASMA - STILL SOME CONFUSION - REPLY', AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY AND MICROBIOLOGY, 16 172-173 (1988) |
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1987 |
Allen RD, Roberts TK, 'The role of spermine in the cytotoxic effects of seminal plasma.', Journal of Immunology, 138 656 (1987)
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1987 |
ROBERTS TK, ADAMSON LM, SMART YC, STANGER JD, MURDOCH RN, 'AN EVALUATION OF PERIPHERAL-BLOOD PLATELET ENUMERATION AS A MONITOR OF FERTILIZATION AND EARLY-PREGNANCY', FERTILITY AND STERILITY, 47 848-854 (1987)
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1987 |
ALLEN RD, ROBERTS TK, 'ROLE OF SPERMINE IN THE CYTOTOXIC EFFECTS OF SEMINAL PLASMA', AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY AND MICROBIOLOGY, 13 4-8 (1987)
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1987 |
ADAMSON LM, SMART YC, STANGER JD, MURDOCH RN, ROBERTS TK, 'MECHANISTIC STUDIES OF EARLY-PREGNANCY ASSOCIATED THROMBOCYTOPENIA (EPAT) IN THE MOUSE', AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY AND MICROBIOLOGY, 13 117-120 (1987)
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1986 |
HANCOCK RJT, 'IMMUNOSUPPRESSION BY SEMINAL PLASMA INVITRO - AN ARTIFACT', IMMUNOLOGY TODAY, 7 356-356 (1986)
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1986 |
SMART YC, COX J, ROBERTS TK, BRINSMEAD MW, BURTON RC, 'DIFFERENTIAL EFFECT OF CIGARETTE-SMOKING ON RECIRCULATING LYMPHOCYTE-T SUBSETS IN PREGNANT-WOMEN', JOURNAL OF IMMUNOLOGY, 137 1-3 (1986)
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1986 |
ALLEN RD, ROBERTS TK, 'IMMUNOSUPPRESSION BY SEMINAL PLASMA INVITRO - AN ARTIFACT', IMMUNOLOGY TODAY, 7 356-357 (1986)
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1986 |
Allen RD, Roberts TK, 'Seminal plasma immunosuppression: An irrelevant biological phenomenon?', Clinical Reproduction and Fertility, 4 353-355 (1986)
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1986 |
ALLEN RD, STEVENSON KL, ROBERTS TK, 'ANALYSIS OF ANTIBODIES AGAINST MOUSE SPERMATOZOA USING AN ENZYME-LINKED-IMMUNOSORBENT-ASSAY (ELISA)', AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY AND MICROBIOLOGY, 10 26-31 (1986)
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1986 |
ALLEN RD, ROBERTS TK, 'THE RELATIONSHIP BETWEEN THE IMMUNOSUPPRESSIVE AND CYTOTOXIC EFFECTS OF HUMAN SEMINAL PLASMA', AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY AND MICROBIOLOGY, 11 59-64 (1986)
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1984 |
PRICE RJ, ROBERTS TK, GREEN D, BOETTCHER B, 'ANTICOMPLEMENTARY ACTIVITY IN HUMAN-SEMEN AND ITS POSSIBLE IMPORTANCE IN REPRODUCTION', AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY, 6 92-98 (1984)
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1983 |
SMART YC, CLANCY RL, ROBERTS TK, FRASER IS, CRIPPS AW, 'A MODE OF ACTION OF IUDS - REPLY', FERTILITY AND STERILITY, 39 114-115 (1983) |
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1983 |
SILBURN PA, KHOO SK, DAUNTER B, HILL R, ROBERTS TK, MACKAY EV, 'TYPES OF IMMUNE-COMPLEXES IN THE ASCITIC FLUID OF WOMEN WITH CARCINOMA OF THE OVARY', INTERNATIONAL ARCHIVES OF ALLERGY AND APPLIED IMMUNOLOGY, 71 219-223 (1983)
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1982 |
SMART YC, FRASER IS, CLANCY RL, ROBERTS TK, CRIPPS AW, 'EARLY-PREGNANCY FACTOR AS A MONITOR FOR FERTILIZATION IN WOMEN WEARING INTRAUTERINE-DEVICES', FERTILITY AND STERILITY, 37 201-204 (1982)
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1982 |
SMART YC, ROBERTS TK, FRASER IS, CRIPPS AW, CLANCY RL, 'VALIDATION OF THE ROSETTE INHIBITION TEST FOR THE DETECTION OF EARLY-PREGNANCY IN WOMEN', FERTILITY AND STERILITY, 37 779-785 (1982)
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1982 |
Smart YC, Fraser IS, Roberts TK, Clancy RL, Cripps AW, 'Fertilization and early pregnancy loss in healthy women attempting conception.', Clinical reproduction and fertility, 1 177-184 (1982)
The rosette inhibition test was used to monitor the activity of an early pregnancy factor (EPF) in sera collected from 18 healthy women who were attempting to conceive. Serial blo... [more]
The rosette inhibition test was used to monitor the activity of an early pregnancy factor (EPF) in sera collected from 18 healthy women who were attempting to conceive. Serial blood samples collected during the luteal phase of 21 menstrual cycles demonstrated the appearance of EPF in 14 of the cycles, of which six showed only transient EPF activity over a 5-10 day period following ovulation. These results indicate a 67% rate of fertilization and a 36% rate of early conceptus losses.
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1981 |
SMART YC, ROBERTS TK, CLANCY RL, CRIPPS AW, 'EARLY-PREGNANCY FACTOR - ITS ROLE IN MAMMALIAN REPRODUCTION - RESEARCH REVIEW', FERTILITY AND STERILITY, 35 397-402 (1981)
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1981 |
SMART YC, CRIPPS AW, CLANCY RL, ROBERTS TK, LOPATA A, SHUTT DA, 'DETECTION OF AN IMMUNOSUPPRESSIVE FACTOR IN HUMAN PRE-IMPLANTATION EMBRYO CULTURES', MEDICAL JOURNAL OF AUSTRALIA, 1 78-79 (1981)
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1980 |
TINNEBERG HR, ROBERTS TK, 'DO HIGH HYDROSTATIC PRESSURES AFFECT THE VIABILITY OF ANIMAL AND HUMAN-SPERMATOZOA', ZUCHTHYGIENE-REPRODUCTION IN DOMESTIC ANIMALS, 15 91-91 (1980) |
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1980 |
TINNEBERG HR, ROBERTS TK, CHENG CY, METTLER L, 'HIGH HYDROSTATIC-PRESSURE AS AN IMPROVEMENT FOR SPERM-CRYOPRESERVATION', ARCHIVES OF ANDROLOGY, 5 42-43 (1980)
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1979 |
TUMBOHOERI AG, ROBERTS TK, 'IMMUNOLOGICAL AND MORPHOLOGICAL CONSEQUENCES OF VASECTOMY IN THE RABBIT', EXPERIENTIA, 35 675-676 (1979)
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1979 |
ROBERTS TK, 'VACCINATION BY NATURAL IMPREGNATION', UMSCHAU IN WISSENSCHAFT UND TECHNIK, 79 103-104 (1979) |
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1978 |
DORSMAN BG, TUMBOHOERI AG, ROBERTS TK, 'DETECTION OF CELL-MEDIATED-IMMUNITY TO SPERMATOZOA IN MICE AND MAN BY LEUKOCYTE ADHERENCE-INHIBITION TEST', JOURNAL OF REPRODUCTION AND FERTILITY, 53 277-283 (1978)
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1978 |
TUMBOHOERI AG, ROBERTS TK, 'INDUCTION OF CELL-MEDIATED-IMMUNITY TO SPERMATOZOA FOLLOWING VASECTOMY IN INBRED MICE', INTERNATIONAL JOURNAL OF ANDROLOGY, 1 81-85 (1978)
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1978 |
MISKO IS, BOETTCHER B, ROBERTS TK, KAY DJ, 'SPERMATOZOAL CELLS IN HUMAN SEMEN DO NOT STIMULATE ALLOGENEIC LEUKOCYTES IN CULTURE', LANCET, 1 560-561 (1978)
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1978 |
TUMBOHOERI AG, ROBERTS TK, 'RESEARCH PROGRESS IN VASECTOMY - BIOMEDICAL REVIEW', EAST AFRICAN MEDICAL JOURNAL, 55 523-529 (1978) |
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1977 |
TUMBOHOERI AG, ROBERTS TK, 'CELL-MEDIATED-IMMUNITY TO SPERMATOZOA FOLLOWING VASECTOMY', THERIOGENOLOGY, 8 166-166 (1977)
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1977 |
COUGHLAN M, BOETTCHER B, ROBERTS T, 'BINDING OF LACTATE-DEHYDROGENASE ISOENZYME X (LDH-X) BY SPERM ANTIBODIES OF INFERTILE HUMANS', THERIOGENOLOGY, 8 205-205 (1977)
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1976 |
BOETTCHER B, NANRA RS, ROBERTS TK, MALLAN M, WATTERSON CA, 'SPECIFICITY AND POSSIBLE ORIGIN OF ANTI-N ANTIBODIES DEVELOPED BY PATIENTS UNDERGOING CHRONIC-HEMODIALYSIS', VOX SANGUINIS, 31 408-415 (1976)
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1976 |
ROBERTS TK, MASSON PL, LAUWERYS R, HEREMANS JF, '2 ESTERASES COMMON TO SEMINAL PLASMA, OTHER EXTERNAL SECRETIONS AND LEUKOCYTES IN MAN', ANDROLOGIA, 8 67-71 (1976)
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1976 |
BOETTCHER B, NANRA RS, MALLAN M, ROBERTS TK, WATTERSON CA, 'ORIGIN AND SPECIFICITY OF ANTI-N-LIKE ANTIBODIES IN SERA OF CHRONIC-HEMODIALYSIS PATIENTS', AUSTRALIAN AND NEW ZEALAND JOURNAL OF MEDICINE, 6 97-97 (1976) |
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1975 |
ROBERTS TK, BOURSNELL JC, 'ISOLATION AND CHARACTERIZATION OF LACTOFERRIN FROM SOW MILK AND BOAR SEMINAL PLASMA', JOURNAL OF REPRODUCTION AND FERTILITY, 42 579-582 (1975)
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1975 |
BUTLER WJ, ROBERTS TK, 'EFFECTS OF SOME PHOSPHATIDYL COMPOUNDS ON BOAR SPERMATOZOA FOLLOWING COLD SHOCK OR SLOW COOLING', JOURNAL OF REPRODUCTION AND FERTILITY, 43 183-187 (1975)
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1974 |
ROBERTS TK, BOURSNELL JC, BROWN AD, 'ROLE OF ZINC IN PROMOTING OPALESCENCE AND COLD PRECIPITATION OF BOAR SEMINAL PLASMA', JOURNAL OF REPRODUCTION AND FERTILITY, 37 373-386 (1974)
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1974 |
BOURSNELL JC, ROBERTS TK, 'ROLE OF ZINC IN PROMOTING OPALESCENCE AND COLD-PRECIPITATION OF BOAR SEMINAL PLASMA', JOURNAL OF REPRODUCTION AND FERTILITY, 36 91-99 (1974)
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1974 |
ROBERTS TK, BOURSNEL JC, WINSOR SE, MUSTILL EA, 'ROLE OF ZINC IN PROMOTING OPALESCENCE AND COLD PRECIPITATION OF BOAR SEMINAL PLASMA .3. FURTHER OBSERVATIONS ON NATURE OF COLD-OPALESCENCE PHENOMENON', JOURNAL OF REPRODUCTION AND FERTILITY, 40 411-422 (1974)
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1974 |
ROBERTS TK, BOURSNELL JC, 'INHIBITORY ACTION OF PHOSPHATIDYL COMPOUNDS ON BOAR SEMINAL HEMAGGLUTININ', JOURNAL OF REPRODUCTION AND FERTILITY, 41 489-492 (1974)
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1973 |
KOBAYASHI K, ROBERTS TK, VAERMAN JP, MASSON PL, HEREMANS JF, 'ESTERASE-ACTIVITY ASSOCIATED WITH SECRETORY IGA AND FREE SECRETORY COMPONENT PREPARATIONS FROM HUMAN MILK', BIOCHIMICA ET BIOPHYSICA ACTA, 317 517-523 (1973)
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1973 |
RAO K, ROBERTS TK, MASSON PL, HEREMANS JF, 'LACTOFERRIN, A MAJOR SOLUBLE-PROTEIN OF BOVINE ESTROUS CERVICAL MUCUS', JOURNAL OF REPRODUCTION AND FERTILITY, 32 89-& (1973)
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1972 |
ROBERTS TK, RAO K, HEREMANS JF, MASSON PL, 'STUDIES ON PROTEINS OF HUMAN SEMINAL PLASMA .1. CHARACTERIZATION OF 2 ESTERASE SYSTEMS', BIOCHIMICA ET BIOPHYSICA ACTA, 278 219-+ (1972)
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1970 |
BOETTCHER B, HAY J, KAY DJ, BALDO BA, ROBERTS TK, 'SPERMAGGLUTINATING ACTIVITY IN SOME HUMAN SERA', INTERNATIONAL JOURNAL OF FERTILITY, 15 143-+ (1970)
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1969 |
ROBERTS TK, BOETTCHER B, 'IDENTIFICATION OF HUMAN SPERM-COATING ANTIGEN', JOURNAL OF REPRODUCTION AND FERTILITY, 18 347-+ (1969)
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