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Dr Mark Baker

Research Fellow

School of Environmental and Life Sciences (Biological Sciences)

Kicking goals

Dr Mark Baker has picked up the pace of sperm cell research by harnessing the power of proteomics.

Mark Baker on the soccer field 

There is a competitive side to Dr Mark Baker, who likes to keep fit and, at 37, still enjoys the challenge of matching strides with younger opponents on the soccer field. But the race Baker is most keen to win is the one to develop a male version of the contraceptive pill – and the proteomics expert believes his research team at the University of Newcastle has a real chance of achieving that goal.

"We are the world leaders in sperm proteomics," points out Baker, who was the first researcher to create a complete inventory of the proteins in sperm and has established a state-of-the-art proteomics laboratory at the University. "The work we do is part of a worldwide effort but we are recognised as pioneers and the most progressive group in our field."

Baker joined the University's influential Reproductive Science Group, led by Laureate Professor John Aitken, after being awarded a PhD from Monash University in 2002. Aitken, who specialises in sperm cell biology, recognised the potential in combining their areas of expertise towards the common goal of solving the mysteries of male fertility. 

Whilst Baker's ultimate goal is to develop a male contraceptive, his research has equal potential to assist men who are infertile, as it is necessary to understand the mechanisms of infertility in order to mimic the condition with a male contraceptive. In identifying and publishing the sperm proteome, Baker advanced the number of known proteins from less than 40 to more than 1200. He and the research team have subsequently narrowed down that list of proteins to a handful that can potentially be targeted by fertility drugs.

"One of the important criteria is that the protein is found only in the testes, because if you are designing a drug that inhibits a protein you don't want it to affect other parts of the body," Baker explains.  "So, we have identified a number of proteins that we know are both unique to the testes and are vital for fertility. We are now moving into the next phase of the research, which is designing a drug to target them."

Baker's work has attracted the attention of Bayer (formerly Bayer Schering Pharma), one of the first companies to market the contraceptive pill back in the 1960s. He has also established collaborations with leading international research groups in the field.

"The implications of our research are widespread when you consider that about half of all pregnancies in Australia are unwanted or unplanned and that there are about 250 abortions for every 1000 live births," Baker asserts. "There are also thousands of women who suffer side effects from contraception and there is an inextricable link between poverty and high birth rates in developing counties. As you can imagine, there is a real global imperative to improve options by developing a male contraceptive. At the same time, up to 10 per cent of the male population is infertile, so we want to be able to help them as well."

Baker has contributed his expertise in identifying the protein structure of cells to other interesting collaborations with leading University health researchers. He is currently working with Professor Hubert Hondermark on a project to detect metastatic potential in breast cancer cells and with Conjoint Professor Jim Denham to determine new biomarkers for prostate cancer. He is also working with members of the translational stroke research team on a project investigating indicators of stroke in cerebral spinal fluid.

Baker holds a National Health and Medical Research Council (NHMRC) Career Development Fellowship to research sperm motility and is an investigator on several projects supported by the NHMRC. Late last year he received the 2011 Early Career Researcher Award sponsored by the Hunter Medical Research Institute (HMRI) fundraising group PULSE.

Baker's research in sperm-egg recognition and proteomic analysis of sperm cells is internationally recognised and has been cited more than 950 times. The proteomics laboratory he was instrumental  in establishing, with its world-class mass spectrometry equipment, has made a broad contribution to research at the University of Newcastle,  supporting work across the disciplines of life science, science, medicine and engineering as well as research from HMRI and international and national collaborations.

Two minds are better than one

The powerful combination of Laureate Professor John Aitken's vast knowledge of sperm cell biology and Dr Mark Baker's proteomics prowess makes the University of Newcastle's Reproductive Science Group a formidable force in the research field of sperm function and male fertility.

"John and I decided seven years ago that we needed to investigate the proteins in sperm cells to understand the mechanisms of fertility," Baker advises. "We saw the potential in it and went after it, and the University has backed us all the way. We quickly got into the field and we have raised the bar."

Baker believes he has benefited greatly from working with Aitken, one of the world's most esteemed reproductive scientists. "He has been my primary mentor, but it is a relationship built on mutual respect," Baker emphasises. "There is a willingness to work together but also a willingness to listen to each other's points of view – and that is critical to successful research: to be able to agree but also disagree."

Aitken describes Baker as"an ideal example of how postdoctoral researchers can blossom into fully-fledged professional scientists when the University supports their careers."Now, Mark is generating research income in his own right that will more than repay the investment the University has made in developing his research portfolio."

Visit the Centre for Reproductive Science website

Visit the HMRI website

Mark Baker on the soccer field

Kicking goals

Dr Mark Baker has picked up the pace of sperm cell research by harnessing the power of proteomics.

Read more

Career Summary

Biography

After obtaining an Australia postgraduate Award, I did my PhD at Monash University, Department of Biochemistry and Cell Biology under the supervision of Dr. Alfons Lawen. Following a successful time, I was invited to the join the group at the University of Newcastle under Prof. John Aitken, which was a continuation of my PhD theme in the identifying plasma membrane redox systems. Within two years, I decided to set-up and establish a proteomics platform at the University of Newcastle and have since been successful in establishing 4 mass spectrometers in the Advanced mass spectrometry facility. In 2005 I was awarded the university of Newcastle Fellowship (rank #1 from 96 applications) which allowed 5 years of full time research. In 2011 I was awarded the NHMRC career development fellowship (level 2) from the NHMRC. I have been successful on 3 NHMRC DP grants, 1 ARC project grant and 3 ARC LIEF grants.

Research Expertise

Proteomics and phospho-proteomics comparisons using label-free mass spectrometry.

Teaching Expertise
6 lectures to Biol 3020 on reproductive physiology.

Administrative Expertise
Grants, animal ethics, Health and Safety.

Collaborations
Nuch Tanphaichitr, Senior Scientist, Ottawa Hospital Research Institute, and Professor in Obstetrics and Gynecology, and Biochemistry/Microbiology/Immunology, University of Ottawa Harvey Florman, University of Massachusetts Melissa Junganickel, University of Massachusetts Gibbes Johnson, Acting Deputy Director, Division of Therapeutic Proteins CDER, FDA, Bethesda University John Aitken, University of Newcastle Neil Sprat, University of Newcastle Tony Velkov, University of Newcastle Brett Nixon, University of Newcastle

Qualifications

  • PhD, Monash University

Keywords

  • Biol 3000
  • Fertilization
  • Phosphorylation
  • Spermatozoa

Fields of Research

Code Description Percentage
060111 Signal Transduction 40
060199 Biochemistry and Cell Biology not elsewhere classified 30
111499 Paediatrics and Reproductive Medicine not elsewhere classified 30

Professional Experience

UON Appointment

Title Organisation / Department
Research Fellow University of Newcastle
School of Environmental and Life Sciences
Australia

Academic appointment

Dates Title Organisation / Department
1/02/2012 - 1/12/2015 Fellow - NHMRC University of Newcastle
School of Environmental and Life Sciences
Australia
1/01/2010 -  NHMRC Career Development Fellow University of Newcastle
School of Environmental and Life Sciences
Australia
1/06/2007 - 1/02/2012 Fellow - UON University of Newcastle
School of Environmental and Life Sciences
Australia
1/01/2005 - 1/01/2011 University of Newcastle Research Fellow University of Newcastle
School of Environmental and Life Sciences
Australia
1/01/2002 - 1/01/2005 Post doc University of Newcastle
School of Elect Engineering and Computer Science
Australia
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Publications

For publications that are currently unpublished or in-press, details are shown in italics.


Chapter (5 outputs)

Year Citation Altmetrics Link
2013 Baker MA, Aitken RJ, 'Mass Spectrometry proteomics and the study of sperm cell biology', Human Gametes and Preimplantation Embryos, Springer, New York, NY 193-203 (2013) [B1]
DOI 10.1007/978-1-4614-6651-2_18
Co-authors John Aitken
2013 Baker MA, 'Phosphoproteomic analysis of peptides', 271-277 (2013) [B2]

Posttranslational modifications represent a major mechanism by which cells control molecular events. Sample preparation is one of the biggest keys in terms of proteomic analysis, ... [more]

Posttranslational modifications represent a major mechanism by which cells control molecular events. Sample preparation is one of the biggest keys in terms of proteomic analysis, and especially when looking at phosphoproteomics. Here we describe two techniques that are used to identify phosphopeptides. These are Titanium Dioxide and Immobilized Metal Affinity Chromatography (IMAC) which enrich for phosphopeptides. These enrichment techniques typically often identify ~35 % of the same and ~65 % unique phosphopeptides. In this chapter, we describe the use of an ion-trap mass spectrometer for the identification of phosphopeptides. © 2013 Springer Science+Business Media, LLC.

DOI 10.1007/978-1-62703-613-9_18
2012 Aitken RJ, De Iuliis GN, Baker MA, 'Direct methods for the detection of reactive oxygen species in human semen samples', Studies on Men's Health and Fertility, Springer, New York 275-299 (2012) [B1]
Co-authors Geoffry DeiuliIs, John Aitken
2010 Aitken RJ, Baker MA, De Iuliis GN, Nixon B, 'New insights into sperm physiology and pathology', Fertility Control, Springer, Berlin 99-115 (2010) [B1]
Citations Scopus - 23
Co-authors Geoffry DeiuliIs, Brett Nixon, John Aitken
2007 Aitken RJ, Roman SD, Baker MA, De Iuliis GN, 'Redox regulation of DNA damage in the male germ line', Male-Mediated Developmental Toxicity, Royal Society of Chemistry, London, United Kingdom 197-209 (2007) [B1]
Citations Web of Science - 2
Co-authors Geoffry DeiuliIs, John Aitken, Shaun Roman
Show 2 more chapters

Journal article (75 outputs)

Year Citation Altmetrics Link
2015 Kongmanas K, Kruevaisayawan H, Saewu A, Sugeng C, Fernandes J, Souda P, et al., 'Proteomic Characterization of Pig Sperm Anterior Head Plasma Membrane Reveals Roles of Acrosomal Proteins in ZP3 Binding', JOURNAL OF CELLULAR PHYSIOLOGY, 230 449-463 (2015)
DOI 10.1002/jcp.24728
Citations Scopus - 1Web of Science - 3
Co-authors John Aitken
2015 Tanphaichitr N, Kongmanas K, Kruevaisayawan H, Saewu A, Sugeng C, Fernandes J, et al., 'Remodeling of the plasma membrane in preparation for sperm-egg recognition: roles of acrosomal proteins', ASIAN JOURNAL OF ANDROLOGY, 17 574-582 (2015)
DOI 10.4103/1008-682X.152817
Co-authors John Aitken
2015 Baker MA, Weinberg A, Hetherington L, Villaverde AI, Velkov T, 'Analysis of protein thiol changes occurring during rat sperm epididymal maturation.', Biol Reprod, 92 11 (2015)
DOI 10.1095/biolreprod.114.123679
Citations Scopus - 1Web of Science - 1
2015 Young SA, Aitken J, Baker MA, 'Phosphorylation of Izumo1 and Its Role in Male Infertility.', Asian J Androl, (2015)
2015 Schneider EK, Huang JX, Carbone V, Baker M, Azad MAK, Cooper MA, et al., 'Drug-drug plasma protein binding interactions of ivacaftor', JOURNAL OF MOLECULAR RECOGNITION, 28 339-348 (2015)
DOI 10.1002/jmr.2447
2015 Aitken RJ, Baker MA, Nixon B, 'Are sperm capacitation and apoptosis the opposite ends of a continuum driven by oxidative stress?', Asian J Androl, 17 633-639 (2015)
DOI 10.4103/1008-682X.153850
Co-authors John Aitken, Brett Nixon
2015 Baker MA, Weinberg A, Hetherington L, Villaverde AI, Velkov T, Baell J, Gordon CP, 'Defining the mechanisms by which the reactive oxygen species by-product, 4-hydroxynonenal, affects human sperm cell function.', Biol Reprod, 92 108 (2015)
DOI 10.1095/biolreprod.114.126680
Citations Web of Science - 1
2015 Carbone V, Schneider EK, Rockman S, Baker M, Huang JX, Ong C, et al., 'Molecular Characterisation of the Haemagglutinin Glycan-Binding Specificity of Egg-Adapted Vaccine Strains of the Pandemic 2009 H1N1 Swine Influenza A Virus', MOLECULES, 20 10415-10434 (2015)
DOI 10.3390/molecules200610415
2015 Baker MA, 'Proteomics of post-translational modifications of mammalian spermatozoa.', Cell Tissue Res, (2015)
DOI 10.1007/s00441-015-2249-x
2014 Baker MA, Hetherington L, Weinberg A, Velkov T, 'Phosphopeptide analysis of rodent epididymal spermatozoa', Journal of visualized experiments : JoVE, (2014) [C1]

Spermatozoa are quite unique amongst cell types. Although produced in the testis, both nuclear gene transcription and translation are switched off once the pre-cursor round cell b... [more]

Spermatozoa are quite unique amongst cell types. Although produced in the testis, both nuclear gene transcription and translation are switched off once the pre-cursor round cell begins to elongate and differentiate into what is morphologically recognized as a spermatozoon. However, the spermatozoon is very immature, having no ability for motility or egg recognition. Both of these events occur once the spermatozoa transit a secondary organ known as the epididymis. During the ~12 day passage that it takes for a sperm cell to pass through the epididymis, post-translational modifications of existing proteins play a pivotal role in the maturation of the cell. One major facet of such is protein phosphorylation. In order to characterize phosphorylation events taking place during sperm maturation, both pure sperm cell populations and pre-fractionation of phosphopeptides must be established. Using back flushing techniques, a method for the isolation of pure spermatozoa of high quality and yield from the distal or caudal epididymides is outlined. The steps for solubilization, digestion, and pre-fractionation of sperm phosphopeptides through TiO2 affinity chromatography are explained. Once isolated, phosphopeptides can be injected into MS to identify both protein phosphorylation events on specific amino acid residues and quantify the levels of phosphorylation taking place during the sperm maturation processes.

DOI 10.3791/51546
2014 Aitken RJ, Smith TB, Jobling MS, Baker MA, De Iuliis GN, 'Oxidative stress and male reproductive health', Asian Journal of Andrology, 16 31-38 (2014) [C1]

One of the major causes of defective sperm function is oxidative stress, which not only disrupts the integrity of sperm DNA but also limits the fertilizing potential of these cell... [more]

One of the major causes of defective sperm function is oxidative stress, which not only disrupts the integrity of sperm DNA but also limits the fertilizing potential of these cells as a result of collateral damage to proteins and lipids in the sperm plasma membrane. The origins of such oxidative stress appear to involve the sperm mitochondria, which have a tendency to generate high levels of superoxide anion as a prelude to entering the intrinsic apoptotic cascade. Unfortunately, these cells have very little capacity to respond to such an attack because they only possess the first enzyme in the base excision repair (BER) pathway, 8-oxoguanine glycosylase 1 (OGG1). The latter successfully creates an abasic site, but the spermatozoa cannot process the oxidative lesion further because they lack the downstream proteins (APE1, XRCC1) needed to complete the repair process. It is the responsibility of the oocyte to continue the BER pathway prior to initiation of S-phase of the first mitotic division. If a mistake is made by the oocyte at this stage of development, a mutation will be created that will be represented in every cell in the body. Such mechanisms may explain the increase in childhood cancers and other diseases observed in the offspring of males who have suffered oxidative stress in their germ line as a consequence of age, environmental or lifestyle factors. The high prevalence of oxidative DNA damage in the spermatozoa of male infertility patients may have implications for the health of children conceivedin vitro and serves as a driver for current research into the origins of free radical generation in the germ line. © 2014 AJA, SIMM & SJTU. All rights reserved.

DOI 10.4103/1008-682X.122203
Citations Scopus - 25Web of Science - 26
Co-authors Geoffry DeiuliIs, John Aitken
2014 Velkov T, Deris ZZ, Huang JX, Azad MAK, Butler M, Sivanesan S, et al., 'Surface changes and polymyxin interactions with a resistant strain of Klebsiella pneumoniae', INNATE IMMUNITY, 20 350-363 (2014) [C1]
DOI 10.1177/1753425913493337
Citations Scopus - 3Web of Science - 3
2014 Baker MA, Hetherington L, Weinberg A, Velkov T, 'Phosphopeptide analysis of rodent epididymal spermatozoa', Journal of visualized experiments : JoVE, (2014) [C1]

Spermatozoa are quite unique amongst cell types. Although produced in the testis, both nuclear gene transcription and translation are switched off once the pre-cursor round cell b... [more]

Spermatozoa are quite unique amongst cell types. Although produced in the testis, both nuclear gene transcription and translation are switched off once the pre-cursor round cell begins to elongate and differentiate into what is morphologically recognized as a spermatozoon. However, the spermatozoon is very immature, having no ability for motility or egg recognition. Both of these events occur once the spermatozoa transit a secondary organ known as the epididymis. During the ~12 day passage that it takes for a sperm cell to pass through the epididymis, post-translational modifications of existing proteins play a pivotal role in the maturation of the cell. One major facet of such is protein phosphorylation. In order to characterize phosphorylation events taking place during sperm maturation, both pure sperm cell populations and pre-fractionation of phosphopeptides must be established. Using back flushing techniques, a method for the isolation of pure spermatozoa of high quality and yield from the distal or caudal epididymides is outlined. The steps for solubilization, digestion, and pre-fractionation of sperm phosphopeptides through TiO2 affinity chromatography are explained. Once isolated, phosphopeptides can be injected into MS to identify both protein phosphorylation events on specific amino acid residues and quantify the levels of phosphorylation taking place during the sperm maturation processes.

DOI 10.3791/51546
2014 Arjomand A, Baker MA, Li C, Buckle AM, Jans DA, Loveland KL, Miyamoto Y, 'The a-importome of mammalian germ cell maturation provides novel insights for importin biology.', FASEB J, 28 3480-3493 (2014) [C1]
DOI 10.1096/fj.13-244913
Citations Scopus - 4Web of Science - 4
2014 Baker MA, Hetherington L, Weinberg A, Velkov T, 'Phosphopeptide Analysis of Rodent Epididymal Spermatozoa', JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, (2014) [C1]
DOI 10.3791/51546
2013 Carbone V, Kim H, Huang JX, Baker MA, Ong C, Cooper MA, et al., 'Molecular characterization of the receptor binding structure-activity relationships of influenza B virus hemagglutinin', Acta Virologica: international journal, 57 313-332 (2013) [C1]
DOI 10.4149/av_2013_03_313
Citations Scopus - 2
2013 Velkov T, Ong C, Baker MA, Kim H, Li J, Nation RL, et al., 'The antigenic architecture of the hemagglutinin of influenza H5N1 viruses', Molecular Immunology, 56 705-719 (2013) [C1]
DOI 10.1016/j.molimm.2013.07.010
Citations Scopus - 8Web of Science - 8
2013 Rockman S, Camuglia S, Vandenberg K, Ong C, Baker MA, Nation RL, et al., 'Reverse engineering the antigenic architecture of the haemagglutinin from influenza H5N1 clade 1 and 2.2 viruses with fine epitope mapping using monoclonal antibodies', Molecular Immunology, 53 435-442 (2013) [C1]
DOI 10.1016/j.molimm.2012.10.001
Citations Scopus - 4Web of Science - 2
2013 Aitken RJ, Baker MA, 'Oxidative stress, spermatozoa and leukocytic infiltration: Relationships forged by the opposing forces of microbial invasion and the search for perfection', Journal of Reproductive Immunology, 100 11-19 (2013) [C1]
DOI 10.1016/j.jri.2013.06.005
Citations Scopus - 7Web of Science - 6
Co-authors John Aitken
2013 Miyamoto Y, Baker MA, Whiley PA, Arjomand A, Ludeman J, Wong C, et al., 'Towards delineation of a developmental alpha-importome in the mammalian male germline', BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH, 1833 731-742 (2013) [C1]
DOI 10.1016/j.bbamcr.2012.11.005
Citations Scopus - 8Web of Science - 7
2013 Aitken RJ, Baker MA, 'Causes and consequences of apoptosis in spermatozoa; contributions to infertility and impacts on development', International Journal of Developmental Biology, 57 265-272 (2013) [C1]

During early development, apoptosis plays a major role in the ontogeny of the germ line as a means of regulating the germ cell:Sertoli cell ratio. In the adult, apoptosis fulfils ... [more]

During early development, apoptosis plays a major role in the ontogeny of the germ line as a means of regulating the germ cell:Sertoli cell ratio. In the adult, apoptosis fulfils another function in removing damaged germ cells from the seminiferous epithelium in response to a wide range of physiological and environmental triggers. These include various forms of electromagnetic radiation, chemotherapeutic agents and commonly encountered toxicants such as phthalate es-ters, bisphenol A and cadmium. This form of apoptosis can lead to spermatogenic arrest and is predominantly mediated by the Fas/FasL system. In addition, senescent mature spermatozoa can undergo a truncated form of apoptosis in order to ensure their efficient phagocytosis within the male and female reproductive tracts. This apoptotic cascade appears to be triggered by oxidative stress and lipid peroxidation, which leads to activation of mitochondrial reactive oxygen species (ROS) generation in a self-perpetuating redox cycle. The electrophilic aldehydes generated as a result of lipid peroxidation also lead to a rapid loss of sperm motility followed some hours later by caspase activation and phosphatidylserine exposure on the sperm surface. The nuclear DNA suffers oxidative damage during this process but there is no immediate DNA cleavage by endonucleases as there is in somatic cells. The reasons for this deviation from the normal pattern of apoptosis involve the unusual physical architecture of spermatozoa and the limited capacity these cells possess for base-excision repair. These findings have practical implications for the approaches that might be used to detect and prevent DNA damage in spermatozoa. © 2013 UBC Press.

DOI 10.1387/ijdb.130146ja
Citations Scopus - 14Web of Science - 13
Co-authors John Aitken
2013 Smith TB, Baker MA, Connaughton HS, Habenicht U, Aitken RJ, 'Functional deletion of Txndc2 and Txndc3 increases the susceptibility of spermatozoa to age-related oxidative stress', Free Radical Biology and Medicine, 65 872-881 (2013) [C1]
DOI 10.1016/j.freeradbiomed.2013.05.021
Citations Scopus - 6Web of Science - 7
Co-authors John Aitken
2013 Baker MA, Weinberg AS, Hetherington L, Velkov T, Aitken RJ, 'Post-ejaculatory changes in the metabolic status of rat spermatozoa as measured by GC-MS', Metabolomics, 9 708-721 (2013) [C1]
DOI 10.1007/s11306-012-0478-5
Co-authors John Aitken
2013 Baker MA, Naumovski N, Hetherington L, Weinberg A, Velkov T, Aitken RJ, 'Head and flagella subcompartmental proteomic analysis of human spermatozoa', Proteomics, 13 61-74 (2013) [C1]
DOI 10.1002/pmic.201200350
Citations Scopus - 31Web of Science - 34
Co-authors John Aitken
2013 Velkov T, Soon RL, Chong PL, Huang JX, Cooper MA, Azad MAK, et al., 'Molecular basis for the increased polymyxin susceptibility of Klebsiella pneumoniae strains with under-acylated lipid A', Innate Immunity, 19 265-277 (2013) [C1]
DOI 10.1177/1753425912459092
Citations Scopus - 3Web of Science - 3
2013 Aitken RJ, Smith TB, Lord T, Kuczera L, Koppers AJ, Naumovski N, et al., 'On methods for the detection of reactive oxygen species generation by human spermatozoa: analysis of the cellular responses to catechol oestrogen, lipid aldehyde, menadione and arachidonic acid', Andrology, 1 192-205 (2013) [C1]
DOI 10.1111/j.2047-2927.2012.00056.x
Citations Scopus - 13Web of Science - 12
Co-authors Geoffry DeiuliIs, John Aitken
2012 Aitken RJ, Whiting SJ, De Iuliis GN, McClymont SL, Mitchell LA, Baker MA, 'Electrophilic aldehydes generated by sperm metabolism activate mitochondrial reactive oxygen species generation and apoptosis by targeting succinate dehydrogenase', Journal of Biological Chemistry, 287 33048-33060 (2012) [C1]
DOI 10.1074/jbc.M112.366690
Citations Scopus - 31Web of Science - 32
Co-authors Geoffry DeiuliIs, John Aitken
2012 Aitken RJ, De Iuliis GN, Gibb Z, Baker MA, 'The Simmet lecture: New horizons on an old landscape - oxidative stress, DNA damage and apoptosis in the male germ line', Reproduction in Domestic Animals, 47 7-14 (2012) [C2]
Citations Scopus - 13Web of Science - 11
Co-authors John Aitken, Geoffry DeiuliIs
2012 Huang JX, Cooper MA, Baker MA, Azad MAK, Nation RL, Li J, Velkov T, 'Drug-binding energetics of human alpha-1-acid glycoprotein assessed by isothermal titration calorimetry and molecular docking simulations', Journal of Molecular Recognition, 25 642-656 (2012) [C1]
Citations Scopus - 4Web of Science - 4
2012 Baker MA, Hetherington L, Weinberg AS, Naumovski N, Velkov T, Pelzing M, et al., 'Analysis of phosphopeptide changes as spermatozoa acquire functional competence in the epididymis demonstrates changes in the post-translational modification of Izumo1', Journal of Proteome Research, 11 5252-5264 (2012) [C1]
DOI 10.1021/pr300468m
Citations Scopus - 5Web of Science - 6
Co-authors John Aitken
2012 Redgrove KA, Nixon B, Baker MA, Hetherington L, Baker G, Liu D-Y, Aitken RJ, 'The molecular chaperone HSPA2 plays a key role in regulating the expression of sperm surface receptors that mediate sperm-egg recognition', Plos One, 7 1-16 (2012) [C1]
Citations Scopus - 20Web of Science - 21
Co-authors John Aitken, Brett Nixon
2012 Baker MA, Nixon B, Naumovski N, Aitken RJ, 'Proteomic insights into the maturation and capacitation of mammalian spermatozoa', Systems Biology in Reproductive Medicine, 58 211-217 (2012) [C1]
DOI 10.3109/19396368.2011.639844
Citations Scopus - 22Web of Science - 20
Co-authors Brett Nixon, John Aitken
2012 Huang JX, Azad MAK, Yuriev E, Baker MA, Nation RL, Li J, et al., 'Molecular characterization of lipopolysaccharide binding to human a-1-acid glycoprotein', Journal of Lipids, - 1-15 (2012) [C1]
2011 Baker MA, Smith ND, Hetherington L, Pelzing M, Condina MR, Aitken RJ, 'Use of titanium dioxide to find phosphopeptide and total protein changes during epididymal sperm maturation', Journal of Proteome Research, 10 1004-1017 (2011) [C1]
DOI 10.1021/pr1007224
Citations Scopus - 22Web of Science - 17
Co-authors John Aitken
2011 Dun MD, Smith ND, Baker MA, Lin M, Aitken RJ, Nixon B, 'The chaperonin containing TCP1 complex (CCT/TRiC) is involved in mediating sperm-oocyte interaction', Journal of Biological Chemistry, 286 36875-36887 (2011) [C1]
DOI 10.1074/jbc.m110.188888
Citations Scopus - 21Web of Science - 20
Co-authors Brett Nixon, Minjie Lin, Matt Dun, John Aitken
2011 Baker MA, 'The 'omics revolution and our understanding of sperm cell biology', Asian Journal of Andrology, 13 6-10 (2011) [C1]
DOI 10.1038/aja.2010.62
Citations Scopus - 22Web of Science - 24
2011 Aitken RJ, Baker MA, 'Cell biology of human spermatozoa: Lessons from the Proteome', Australian Biochemist, 42 12-15 (2011) [C3]
Co-authors John Aitken
2010 Baker MA, Smith ND, Hetherington L, Taubman K, Graham ME, Robinson PJ, Aitken RJ, 'Label-free quantitation of phosphopeptide changes during rat sperm capacitation', Journal of Proteome Research, 9 718-729 (2010) [C1]
DOI 10.1021/pr900513d
Citations Scopus - 25Web of Science - 19
Co-authors John Aitken
2010 Baker MA, Reeves GM, Hetherington L, Aitken RJ, 'Analysis of proteomic changes associated with sperm capacitation through the combined use of IPG-strip pre-fractionation followed by RP chromatography LC-MS/MS analysis', Proteomics, 10 482-495 (2010) [C1]
DOI 10.1002/pmic.200900574
Citations Scopus - 34Web of Science - 34
Co-authors John Aitken
2009 Baker MA, Aitken RJ, 'Proteomic insights into spermatozoa: Critiques, comments and concerns', Expert Review of Proteomics, 6 691-705 (2009) [C1]
DOI 10.1586/epr.09.76
Citations Scopus - 30Web of Science - 27
Co-authors John Aitken
2009 Ewen KA, Baker MA, Wilhelm D, Aitken RJ, Koopman P, 'Global survey of protein expression during gonadal sex determination in mice', Molecular & Cellular Proteomics, 8 2624-2641 (2009) [C1]
DOI 10.1074/mcp.m900108-mcp200
Citations Scopus - 8Web of Science - 7
Co-authors John Aitken
2009 Baker MA, Hetherington L, Curry BJ, Aitken RJ, 'Phosphorylation and consequent stimulation of the tyrosine kinase c-Abl by PKA in mouse spermatozoa: Its implications during capacitation', Developmental Biology, 333 57-66 (2009) [C1]
DOI 10.1016/j.ydbio.2009.06.022
Citations Scopus - 25Web of Science - 25
Co-authors Ben Curry, John Aitken
2008 Mitchell LA, Nixon B, Baker MA, Aitken RJ, 'Investigation of the role of SRC in capacitation-associated tyrosine phosphorylation of human spermatozoa', Molecular Human Reproduction, 14 235-243 (2008) [C1]
DOI 10.1093/molehr/gan007
Citations Scopus - 46Web of Science - 46
Co-authors John Aitken, Brett Nixon
2008 Baker MA, Hetherington L, Reeves GM, Muller J, Aitken RJ, 'The rat sperm proteome characterized via IPG strip prefractionation and LC-MS/MS identification', Proteomics, 8 2312-2321 (2008) [C1]
DOI 10.1002/pmic.200700876
Citations Scopus - 89Web of Science - 66
Co-authors John Aitken
2008 Baker MA, Hetherington L, Reeves GM, Aitken RJ, 'The mouse sperm proteome characterized via IPG strip prefractionation and LC-MS/MS identification', Proteomics, 8 1720-1730 (2008) [C1]
DOI 10.1002/pmic.200701020
Citations Scopus - 12Web of Science - 77
Co-authors John Aitken
2008 Aitken RJ, Hughes LM, Griffith R, Baker MA, 'Bridging the gap between male and female fertility control; contraception-on-demand', Contraception, 78 S28-S35 (2008) [C1]
DOI 10.1016/j.contraception.2008.03.020
Citations Scopus - 8Web of Science - 4
Co-authors John Aitken
2008 Aitken RJ, Baker MA, Doncel GF, Matzuk MM, Mauck CK, Harper MJK, 'As the world grows: Contraception in the 21st century', Journal of Clinical Investigation, 118 1330-1343 (2008) [C1]
DOI 10.1172/jci33873
Citations Scopus - 41Web of Science - 29
Co-authors John Aitken
2008 Aitken RJ, Baker MA, 'The role of proteomics in understanding sperm cell biology', International Journal of Andrology, 31 295-302 (2008) [C1]
DOI 10.1111/j.1365-2605.2007.00851.x
Citations Scopus - 62Web of Science - 59
Co-authors John Aitken
2007 Aitken RJ, Nixon B, Lin M, Koppers AJ, Lee YH, Baker MA, 'Proteomic changes in mammalian spermatozoa during epididymal maturation', Asian Journal of Andrology, 9 554-564 (2007) [C1]
DOI 10.1111/j.1745-7262.2007.00280.x
Citations Scopus - 71Web of Science - 63
Co-authors Minjie Lin, John Aitken, Brett Nixon
2007 Baker MA, Reeves GE, Hetherington L, Muller J, Baur I, Aitken RJ, 'Identification of gene products present in Triton X-100 soluble and insoluble fractions of human spermatozoa lysates using LC-MS/MS analysis', Proteomics Clinical Applications, 1 524-532 (2007) [C1]
DOI 10.1002/prca.200601013
Citations Scopus - 98Web of Science - 97
Co-authors John Aitken
2006 Baker MA, Hetherington L, Aitken RJ, 'Identification of SRC as a key PKA-stimulated tyrosine kinase involved in the capacitation-associated hyperactivation of murine spermatozoa', Journal of Cell Science, 119 3182-3192 (2006) [C1]
DOI 10.1242/jcs.03055
Citations Scopus - 101Web of Science - 102
Co-authors John Aitken
2006 Aitken RJ, Baker MA, 'Oxidative stress, sperm survival and fertility control', Molecular and Cellular Endocrinology, 250 66-69 (2006) [C1]
DOI 10.1016/j.mce.2005.12.026
Citations Scopus - 183Web of Science - 166
Co-authors John Aitken
2006 Lin M, Lee YH, Xu W, Baker MA, Aitken RJ, 'Ontogeny of tyrosine phosphorylation-signaling pathways during spermatogenesis and epididymal maturation in the mouse', Biology of Reproduction, 75 588-597 (2006) [C1]
DOI 10.1095/biolreprod.106.052712
Citations Scopus - 16Web of Science - 16
Co-authors John Aitken, Minjie Lin
2005 Baker MA, Aitken RJ, 'Reactive oxygen species in spermatozoa: methods for monitoring and significance for the origins of genetic disease and infertility', Reproductive Biology and Endocrinology, 3 9 (2005) [C1]
DOI 10.1186/1477-7827-3-67
Citations Scopus - 95Web of Science - 82
Co-authors John Aitken
2005 Baker MA, Krutskikh A, Curry BJ, Hetherington L, Aitken RJ, 'Identification of cytochrome-b5 reductase as the enzyme responsible for NADH-dependent lucigenin chemiluminescence in human spermatozoa', Biology of Reproduction, 73 334-342 (2005) [C1]
DOI 10.1095/biolreprod.104.037960
Citations Scopus - 25Web of Science - 25
Co-authors John Aitken, Ben Curry
2005 Baker MA, Witherdin RF, Hetherington L, Cunningham KA, Aitken RJ, 'Identification of post-translational modifications that occur during sperm maturation using difference in two-dimensional gel electrophoresis', Proteomics, 5 1003-1012 (2005) [C1]
DOI 10.1002/pmic.200401100
Citations Scopus - 72Web of Science - 71
Co-authors John Aitken
2004 Baker MA, Attard B, Aitken RJ, 'TWO- DIMENSIONAL DIFFERENTIAL IN GEL ELECTROPHORESIS ( A NOVEL METHOD FOR HIGH THROUGHPUT PROTEOMICS', Australian Biochemist, 35 40-42 (2004)
2004 Baker MA, Krutskikh A, Curry BJ, McLaughlin EA, Aitken RJ, 'Identification of Cytochrome P450-Reductase as the Enzyme Responsible for NADPH-Dependent Lucigenin and Tetrazolium Salt Reduction in Rat Epididymal Sperm Preparations', Biology of Reproduction, 71 307-318 (2004) [C1]
DOI 10.1095/biolreprod.104.027748
Citations Scopus - 35Web of Science - 34
Co-authors John Aitken, Ben Curry, Eileen Mclaughlin
2004 Baker MA, Lane DJR, Ly JD, De Pinto V, Lawen A, 'VDAC1 Is a Transplasma Membrane NADH-Ferricyanide Reductase', Journal of Biological Chemistry, 279 4811-4819 (2004)

Porin isoform 1 or VDAC (voltage-dependent anion-selective channel) 1 is the predominant protein in the outer mitochondrial membrane. We demonstrated previously that a plasma memb... [more]

Porin isoform 1 or VDAC (voltage-dependent anion-selective channel) 1 is the predominant protein in the outer mitochondrial membrane. We demonstrated previously that a plasma membrane NADH-ferricyanide reductase activity becomes up-regulated upon mitochondrial perturbation, and therefore suggested that it functions as a cellular redox sensor. VDAC1 is known to be expressed in the plasma membrane; however, its function there remained a mystery. Here we show that VDAC1, when expressed in the plasma membrane, functions as a NADH-ferricyanide reductase. VDAC1 preparations purified from both plasma membrane and mitochondria fractions exhibit NADH-ferricyanide reductase activity, which can be immunoprecipitated with poly- and monoclonal antibodies directed against VDAC(1). Transfecting cells with pl-VDAC1. GFP, which carries an N-terminal signal peptide, directs VDAC1 to the plasma membrane, as shown by confocal microscopy and FACS analysis, and significantly increases the plasma membrane NADH-ferricyanide reductase activity of the transfected cells. This novel enzymatic activity of the well known VDAC1 molecule may provide an explanation for its role in the plasma membrane. Our data suggest that a major function of VDAC1 in the plasma membrane is that of a NADH(-ferricyanide) reductase that may be involved in the maintenance of cellular redox homeostasis.

DOI 10.1074/jbc.M311020200
Citations Scopus - 124Web of Science - 113
2004 Baker MA, Hetherington L, Ecroyd HW, Roman SD, Aitken RJ, 'Analysis of the mechanism by which calcium negatively regulates the tyrosine phoshorylation cascade associated with sperm capacitation', Journal of Cell Science, 117 211-222 (2004) [C1]
DOI 10.1242/jcs.00842
Co-authors Shaun Roman, John Aitken
2004 Aitken RJ, Baker MA, O'Bryan M, 'Shedding Light on Chemiluminescence: The Application of Chemiluminescence in Diagnostic Andrology', Journal of Andrology, 25 455-465 (2004) [C1]
Citations Scopus - 31Web of Science - 34
Co-authors John Aitken
2004 Baker MA, Aitken RJ, 'The importance of redox regulated pathways in sperm cell biology', Molecular and Cellular Endocrinology, 216 47-54 (2004) [C1]
DOI 10.1016/j.mce.2003.10.068
Citations Scopus - 93Web of Science - 86
Co-authors John Aitken
2004 Aitken RJ, Ryan AL, Baker MA, McLaughlin EA, 'Redox activity associated with the maturation and capacitation of mammalian spermatozoa', Free Radical Biology and Medicine, 36 994-1010 (2004) [C1]
DOI 10.1016/j.freeradbiomed.2004.01.017
Citations Scopus - 75Web of Science - 66
Co-authors John Aitken, Eileen Mclaughlin
2004 Baker MA, Ly JD, Lawen A, 'Characterization of VDAC1 as a plasma membrane NADH-oxidoreductase', BIOFACTORS, 21 215-221 (2004)
Citations Scopus - 15Web of Science - 13
2004 Aitken RJ, Baker MA, 'Oxidative stress and male reproductive biology', Reproduction, Fertility and Development, 16 581-588 (2004) [C1]
DOI 10.1071/RD03089
Citations Scopus - 132Web of Science - 122
Co-authors John Aitken
2004 Sidhu KS, Mate KE, Gunasekera T, Veal D, Hetherington L, Baker MA, et al., 'A flow cytometric assay for global estimation of tyrosine phosphorylation associated with capacitation of spermatozoa from two marsupial species, the tammar wallaby (Macropus eugenii) and the brushtail possum (Trichosurus vulpecula)', Reproduction, 127 95-103 (2004) [C1]
DOI 10.1530/rep.1.00073
Citations Scopus - 18Web of Science - 19
Co-authors John Aitken, Karen Mate, John Rodger
2003 Aitken RJ, Ryan AL, Curry BJ, Baker MA, 'Multiple forms of redox activity in populations of human spermatozoa', Molecular Human Reproduction, 9 645-661 (2003) [C1]
DOI 10.1093/molehr/gag086
Citations Scopus - 58Web of Science - 55
Co-authors John Aitken, Ben Curry
2003 Aitken RJ, Baker MA, Sawyer DE, 'Oxidative stress in the male germ line and its role in the aetiology of male infertility and genetic disease', Reproductive BioMedicine, 7 65-70 (2003) [C1]
Citations Scopus - 135
Co-authors John Aitken
2003 Baker MA, 'New functions of an old protein: The eukaryotic porrin or voltage dependent anion selective channel (VDAC)', Italian Journal of Biochemistry, 17-24 (2003)
2003 Baker MA, Krutskikh A, Aitken RJ, 'Biochemical entities involved in reactive oxygen species generation by human spermatozoa', Protoplasma, 145-151 (2003) [C1]
DOI 10.1007/s00709-002-0057-0
Citations Scopus - 25Web of Science - 24
Co-authors John Aitken
2003 Baker MA, Lewis B, Hetherington L, Aitken RJ, 'Development of the Signalling Pathways Associated With Sperm Capacitation During Epididymal Maturation', Molecular Reproduction and Development, 446-457 (2003) [C1]
DOI 10.1002/mrd.10255
Citations Scopus - 24Web of Science - 23
Co-authors John Aitken
2002 Aitken RJ, Baker MA, 'Reactive oxygen species generation by human spermatozoa: a continuing enigma', International Journal of Andrology, 25 191-194 (2002) [C1]
Citations Scopus - 38Web of Science - 35
Co-authors John Aitken
2002 Baker MA, Grubb D, Lawen A, 'Didemnin B induces apoptosis in proliferating but not resting peripheral blood mononuclear cells', Apoptosis: an international journal on programmed cell death, 407-412 (2002) [C1]
2002 Baker MA, Grubb DR, Lawen A, 'Didemnin B induces apoptosis in proliferating but not resting peripheral blood mononuclear cells', APOPTOSIS, 7 407-412 (2002) [C1]
DOI 10.1023/A:1020078907108
Citations Scopus - 11Web of Science - 8
2000 Baker MA, Lawen A, 'Plasma Membrane NADH-Oxidoreductase System: A Critical Review of the Structural and Functional Data', ANTIOXIDANTS & REDOX SIGNALING, 2 197-212 (2000)
DOI 10.1089/ars.2000.2.2-197
Citations Scopus - 45Web of Science - 39
Show 72 more journal articles

Conference (19 outputs)

Year Citation Altmetrics Link
2013 Baker M, Aitken J, 'ANALYSIS OF PHOSPHOPEPTIDE CHANGES AS SPERMATOZOA ACQUIRE FUNCTIONAL COMPETENCE IN THE EPIDIDYMIS DEMONSTRATES CHANGES IN THE POST-TRANSLATIONAL MODIFICATION OF IZUMO1', ANDROLOGY (2013) [E3]
Co-authors John Aitken
2012 Nixon B, Redgrove KA, Baker MA, Aitken RJ, 'The role of molecular chaperones in regulating human sperm-egg recognition', Abstracts. The Annual Scientific Meeting of the Endocrine Society of Australia and the Society for Reproductive Biology 2012, Gold Coast, QLD (2012) [E3]
Co-authors John Aitken, Brett Nixon
2012 Aitken RJ, Nixon B, Redgrove KA, Dun M, Baker MA, 'The molecular origins of defective sperm function', HUMAN REPRODUCTION, Istanbul, TURKEY (2012) [E3]
Co-authors Matt Dun, Brett Nixon, John Aitken
2012 Kongmanas K, Sugeng C, Souda P, Faull K, Kitajima K, Aitken RJ, et al., 'Proteomic analysis of sperm anterior head plasma membrane: Unfolding the molecular components underlying sperm-egg interaction', Reproduction in Domestic Animals, Vancouver, Canada (2012) [E3]
Co-authors John Aitken
2008 Baker MA, Hetherington L, Reeves GM, Smith ND, Aitken RJ, 'Comparative and phospho proteomics: Identification of protein changes during sperm maturation', Proceedings of 41st Annual Meeting of the Society for the Study of Reproduction. "Reproductive Biology: Basic Discoveries that Affect Our Lives", Kailua-Kona, Hawaii (2008) [E3]
Co-authors John Aitken
2008 Mitchell L, Nixon B, Baker M, Aitken R, 'Investigation of the role of SRC in capacitation associated tyrosine phosphorylation of human spermatozoa', BIOLOGY OF REPRODUCTION, Kona, HI (2008) [E3]
Co-authors Brett Nixon
2008 Baker M, Hetherington L, Reeves G, Smith N, Aitken R, 'Comparative and phospho proteomics: Identification of protein changes during sperm maturation', BIOLOGY OF REPRODUCTION, Kona, HI (2008) [E3]
2007 Baker MA, 'The proteomic analysis of sperm maturation', Gordon Research Conferences: Fertilization & Activation of Development. Program, Plymouth, UK (2007) [E3]
2006 Baker MA, Reeves GM, Aitken RJ, 'New approaches towads the proteomic analysis of the human spermatozoon', JOURNAL OF ANDROLOGY, Chicago, IL (2006)
Co-authors John Aitken
2006 Aitken RJ, Baker MA, Lee YH, Lin M, Nixon B, 'Proteomic Changes in Mouse Spermatozoa During Epididymal Transit', Abstracts, Chatel-Guyon, Auvergne, France (2006) [E3]
Co-authors John Aitken, Brett Nixon, Minjie Lin
2006 Baker MA, Reeves GE, Aitken RJ, 'New Approaches Towards the Proteomic Analysis of the Human Spermatozoon', Book of Abstracts, Lorne, Victoria, Australia (2006) [E3]
Co-authors John Aitken
2006 Baker MA, Curry BJ, Aitken RJ, 'Inactivation of C-ABL Via PKA During Capacitation of Mouse Spermatozoa', Book of Abstracts, Lorne, Victoria, Australia (2006) [E3]
Co-authors John Aitken, Ben Curry
2006 Baker MA, Reeves GM, Aitken RJ, 'New approaches towards the proteomic analysis of the human spermatozoon', Book of Abstracts, Chicago, Illinois (2006) [E3]
Co-authors John Aitken
2005 Baker MA, Aitken RJ, 'Identification of PP60C-SRC as the enzyme responsible for tyrosine phosphorylation and consequent hyperactivation during mouse sperm capacitation', JOURNAL OF ANDROLOGY, Seattle, WA (2005)
Co-authors John Aitken
2005 Baker MA, Aitken RJ, 'Identification of PP60C-SRC as the enzyme responsible for tyrosine phosphorylation and consequent hyperactivation during mouse sperm capacitation', JOURNAL OF ANDROLOGY, Seattle, WA (2005)
Co-authors John Aitken
2003 Baker MA, Hetherington L, Ecroyd HW, Roman SD, Aitken RJ, 'Analysis of the mechanism by which calcium negatively regulates the tyrosine phosphorylation cascade associated with sperm capacitation', ComBio Combined Conference Abstracts, Melbourne (2003) [E3]
Citations Scopus - 65Web of Science - 57
Co-authors Shaun Roman, John Aitken
2002 Baker MA, Krutskikh A, Aitken RJ, 'Redox regulation of sperm function', Proceedings of the Thirty-third annual Conference of the Society for Reproductive Biology, Adelaide, SA (2002) [E3]
Co-authors John Aitken
2002 Aitken RJ, Baker MA, Krutskikh A, 'Oxidative stress and redox regulation in the epididymis', Epididymis III. Third International Conference on the Epididymis., Charlottesville, Virginia, USA (2002) [E3]
Co-authors John Aitken
1998 Lawen A, Baker MA, Malik S, 'Apoptosis and redox homostasis: on a possible mechanism of action of Bcl-2', PROTOPLASMA, UNIV ANTWERP, ANTWERP, BELGIUM (1998)
DOI 10.1007/BF01279288
Citations Scopus - 7Web of Science - 6
Show 16 more conferences
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Grants and Funding

Summary

Number of grants 26
Total funding $6,064,682

Click on a grant title below to expand the full details for that specific grant.


20152 grants / $801,477

Characterising a newly identified mechanism causing elevation of intracranial pressure after acute neurological injury$494,400

Funding body: NHMRC (National Health & Medical Research Council)

Funding body NHMRC (National Health & Medical Research Council)
Project Team Doctor Neil Spratt, Doctor Mark Baker
Scheme Project Grant
Role Investigator
Funding Start 2015
Funding Finish 2015
GNo G1400231
Type Of Funding Aust Competitive - Commonwealth
Category 1CS
UON Y

Izumo1 and its role in male infertility and male contraception$307,077

Funding body: NHMRC (National Health & Medical Research Council)

Funding body NHMRC (National Health & Medical Research Council)
Project Team Doctor Mark Baker, Laureate Professor John Aitken, Dr Christopher Gordon
Scheme Project Grant
Role Lead
Funding Start 2015
Funding Finish 2015
GNo G1400190
Type Of Funding Aust Competitive - Commonwealth
Category 1CS
UON Y

20132 grants / $314,000

Triple Quadrupole Mass Spectrometer with nano-infusion for targeted protein quantification$300,000

Funding body: University of Newcastle

Funding body University of Newcastle
Project Team Doctor Mark Baker, Laureate Professor John Aitken, Conjoint Professor Bogdan Dlugogorski, Professor Hubert Hondermarck
Scheme Equipment Grant
Role Lead
Funding Start 2013
Funding Finish 2013
GNo G1200137
Type Of Funding Internal
Category INTE
UON Y

NucleoCounter SP100$14,000

Funding body: NHMRC (National Health & Medical Research Council)

Funding body NHMRC (National Health & Medical Research Council)
Project Team Laureate Professor John Aitken, Associate Professor Brett Nixon, Doctor Shaun Roman, Doctor Mark Baker, Doctor Geoffry De Iuliis, Doctor Lisa Mitchell, Doctor Matthew Jobling, Doctor Zamira Gibb, Mr Nenad Naumovski
Scheme Equipment Grant
Role Investigator
Funding Start 2013
Funding Finish 2013
GNo G1201175
Type Of Funding Other Public Sector - Commonwealth
Category 2OPC
UON Y

20123 grants / $457,568

Understanding sperm motility for infertility and contraceptive purposes$432,568

Funding body: NHMRC (National Health & Medical Research Council)

Funding body NHMRC (National Health & Medical Research Council)
Project Team Doctor Mark Baker
Scheme Career Development Fellowships
Role Lead
Funding Start 2012
Funding Finish 2012
GNo G1100192
Type Of Funding Aust Competitive - Commonwealth
Category 1CS
UON Y

BMG SPECTROstar Nano$15,000

Funding body: NHMRC (National Health & Medical Research Council)

Funding body NHMRC (National Health & Medical Research Council)
Project Team Laureate Professor John Aitken, Professor Eileen McLaughlin, Ms Belinda Nixon, Doctor Shaun Roman, Doctor Mark Baker, Doctor Geoffry De Iuliis, Doctor Lisa Mitchell, Doctor Matthew Jobling
Scheme Equipment Grant
Role Investigator
Funding Start 2012
Funding Finish 2012
GNo G1100971
Type Of Funding Other Public Sector - Commonwealth
Category 2OPC
UON Y

PULSE Early Career Researcher$10,000

Funding body: Hunter Medical Research Institute

Funding body Hunter Medical Research Institute
Project Team Doctor Mark Baker
Scheme PULSE Early Career Researcher of the Year Award
Role Lead
Funding Start 2012
Funding Finish 2012
GNo G1200578
Type Of Funding Grant - Aust Non Government
Category 3AFG
UON Y

20115 grants / $1,735,222

Chemical principles underpinning a spermostatic-microbiostatic agent capable of preventing pregnancy and the spread of sexually transmitted disease$735,000

Funding body: ARC (Australian Research Council)

Funding body ARC (Australian Research Council)
Project Team Laureate Professor John Aitken, Doctor Mark Baker, Professor Kenneth Beagley, Professor Adam McCluskey
Scheme Discovery Projects
Role Investigator
Funding Start 2011
Funding Finish 2011
GNo G1000173
Type Of Funding Aust Competitive - Commonwealth
Category 1CS
UON Y

Understanding the Molecular Basis of Epididymal Maturation: How Does the Epididymis Modify Spermatozoa, Allowing them to Recognise the Egg?$565,222

Funding body: NHMRC (National Health & Medical Research Council)

Funding body NHMRC (National Health & Medical Research Council)
Project Team Doctor Mark Baker
Scheme Project Grant
Role Lead
Funding Start 2011
Funding Finish 2011
GNo G1000306
Type Of Funding Aust Competitive - Commonwealth
Category 1CS
UON Y

An Advanced Mass Spectrometer for Applications in Phospho-Proteomics, Glycomics and Top-Down Sequencing of Proteins$250,000

Funding body: ARC (Australian Research Council)

Funding body ARC (Australian Research Council)
Project Team Doctor Mark Baker, Laureate Professor John Aitken, Professor Roger Smith, Conjoint Professor Keith Jones, Conjoint Professor Ray Rose, Professor Lois Salamonsen
Scheme Linkage Infrastructure Equipment & Facilities (LIEF)
Role Lead
Funding Start 2011
Funding Finish 2011
GNo G1000632
Type Of Funding Scheme excluded from IGS
Category EXCL
UON Y

3D Ion Trap Mass Spectrometer with ETD for Top Down Proteomics Applications$170,000

Funding body: University of Newcastle

Funding body University of Newcastle
Project Team Doctor Mark Baker, Laureate Professor John Aitken
Scheme Equipment Grant
Role Lead
Funding Start 2011
Funding Finish 2011
GNo G1000470
Type Of Funding Internal
Category INTE
UON Y

An Advanced Mass Spectrometer for Applications in Phospho-Proteomics, Glycomics and Top-Down Sequencing of Proteins$15,000

Funding body: Prince Henry's Institute of Medical Research

Funding body Prince Henry's Institute of Medical Research
Project Team Doctor Mark Baker, Laureate Professor John Aitken, Professor Roger Smith, Conjoint Professor Keith Jones, Conjoint Professor Ray Rose, Professor Lois Salamonsen
Scheme Linkage Infrastructure Equipment & Facilities (LIEF) Partner funding
Role Lead
Funding Start 2011
Funding Finish 2011
GNo G1100844
Type Of Funding Scheme excluded from IGS
Category EXCL
UON Y

20105 grants / $837,000

An Integrated LC-MS-NMR facility for Applications in Proteomics and Organic Chemistry$500,000

Funding body: ARC (Australian Research Council)

Funding body ARC (Australian Research Council)
Project Team Professor Adam McCluskey, Laureate Professor John Aitken, Professor Paul Dastoor, Professor Phillip Robinson, Professor Eileen McLaughlin, Emeritus Professor Geoffrey Lawrance, Professor Marcel Maeder, Professor Hugh Dunstan, Doctor Shaun Roman, Professor Rob Atkin, Doctor Clovia Holdsworth, Doctor Mark Baker, Doctor Nikki Verrills, Professor Gottfried Otting, Associate Professor Brett Nixon, Doctor Xiaojing Zhou, Ms Megan Chircop, Doctor Warwick Belcher
Scheme Linkage Infrastructure Equipment & Facilities (LIEF)
Role Investigator
Funding Start 2010
Funding Finish 2010
GNo G0190402
Type Of Funding Scheme excluded from IGS
Category EXCL
UON Y

An Integrated LC-MS-NMR facility for Applications in Proteomics and Organic Chemistry$280,000

Funding body: University of Newcastle

Funding body University of Newcastle
Project Team Professor Adam McCluskey, Laureate Professor John Aitken, Professor Paul Dastoor, Professor Phillip Robinson, Professor Eileen McLaughlin, Emeritus Professor Geoffrey Lawrance, Professor Marcel Maeder, Professor Hugh Dunstan, Doctor Shaun Roman, Professor Rob Atkin, Doctor Clovia Holdsworth, Doctor Mark Baker, Doctor Nikki Verrills, Professor Gottfried Otting, Associate Professor Brett Nixon, Doctor Xiaojing Zhou, Ms Megan Chircop, Doctor Warwick Belcher
Scheme Equipment Grant
Role Investigator
Funding Start 2010
Funding Finish 2010
GNo G1000873
Type Of Funding Internal
Category INTE
UON Y

Waters, Ultra high performance liquid chromatography system (nano-Aquity) $30,000

Funding body: NHMRC (National Health & Medical Research Council)

Funding body NHMRC (National Health & Medical Research Council)
Project Team Doctor Mark Baker, Laureate Professor John Aitken, Professor Adam McCluskey, Associate Professor Brett Nixon, Emeritus Professor John Rostas, Professor Eileen McLaughlin
Scheme Equipment Grant
Role Lead
Funding Start 2010
Funding Finish 2010
GNo G1000051
Type Of Funding Other Public Sector - Commonwealth
Category 2OPC
UON Y

The design of novel, affinity-based technology to specifically isolate phosphotyrosine-containing pedtides$25,000

Funding body: Hunter Children`s Research Foundation

Funding body Hunter Children`s Research Foundation
Project Team Doctor Mark Baker, Professor Adam McCluskey
Scheme Research Grant
Role Lead
Funding Start 2010
Funding Finish 2010
GNo G0900191
Type Of Funding Contract - Aust Non Government
Category 3AFC
UON Y

2009 FSIT Award for Research Excellence$2,000

Funding body: University of Newcastle

Funding body University of Newcastle
Project Team Doctor Mark Baker
Scheme Award for Research Excellence
Role Lead
Funding Start 2010
Funding Finish 2010
GNo G1000404
Type Of Funding Internal
Category INTE
UON Y

20095 grants / $1,192,621

Identification and characterisation of phosphorylation events taking place during human sperm capacitation$269,250

Funding body: NHMRC (National Health & Medical Research Council)

Funding body NHMRC (National Health & Medical Research Council)
Project Team Doctor Mark Baker
Scheme Project Grant
Role Lead
Funding Start 2009
Funding Finish 2009
GNo G0188834
Type Of Funding Aust Competitive - Commonwealth
Category 1CS
UON Y

China-Australia therapeutic hypothermia in stroke (CATHS) research program: identification of diagnostic molecular markers and therapeutic targets involved in re-warming related brain injury$230,275

Funding body: NSW Office for Science & Medical Research

Funding body NSW Office for Science & Medical Research
Project Team Conjoint Professor Chris Levi, Doctor Mark Baker, Doctor Neil Spratt, Emeritus Professor John Rostas
Scheme China-NSW Collaborative Research Program
Role Investigator
Funding Start 2009
Funding Finish 2009
GNo G0190392
Type Of Funding Other Public Sector - State
Category 2OPS
UON Y

Assessing the significance of the sperm thioredoxins as contraceptive targets for the male$148,096

Funding body: Contraceptive Research and Development Program

Funding body Contraceptive Research and Development Program
Project Team Laureate Professor John Aitken, Doctor Mark Baker
Scheme Development of New or Improved Contraceptive Products
Role Investigator
Funding Start 2009
Funding Finish 2009
GNo G0189339
Type Of Funding International - Non Competitive
Category 3IFB
UON Y

20081 grants / $4,000

PULSE Education Prize$4,000

Funding body: Hunter Medical Research Institute

Funding body Hunter Medical Research Institute
Project Team Doctor Mark Baker
Scheme PULSE Education Prize
Role Lead
Funding Start 2008
Funding Finish 2008
GNo G0188535
Type Of Funding Contract - Aust Non Government
Category 3AFC
UON Y

20073 grants / $722,794

2007 Research Fellowship - PRCRS$509,053

Funding body: University of Newcastle

Funding body University of Newcastle
Project Team Doctor Mark Baker
Scheme Research Fellowship
Role Lead
Funding Start 2007
Funding Finish 2007
GNo G0187158
Type Of Funding Internal
Category INTE
UON Y

AMPPAII Proposal for completion of the Sptrx KO mice study$198,741

Funding body: Schering AG

Funding body Schering AG
Project Team Laureate Professor John Aitken, Doctor Mark Baker
Scheme Research Networks
Role Investigator
Funding Start 2007
Funding Finish 2007
GNo G0188468
Type Of Funding Contract - Aust Non Government
Category 3AFC
UON Y

2007 Research Fellowship Project Grant$15,000

Funding body: University of Newcastle

Funding body University of Newcastle
Project Team Doctor Mark Baker
Scheme Fellowship Grant
Role Lead
Funding Start 2007
Funding Finish 2007
GNo G0188117
Type Of Funding Internal
Category INTE
UON Y
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Research Supervision

Number of supervisions

Completed0
Current2

Total current UON EFTSL

PhD0.35

Current Supervision

Commenced Level of Study Research Title / Program / Supervisor Type
2014 PhD Determining Factors within Cerebrospinal Fluid that Cause Intracranial Pressure to Rise Post-Stroke
Human Biology, Faculty of Health and Medicine, The University of Newcastle
Co-Supervisor
2013 PhD Generation of Gene-Disrupted Mice to Further Elucidate Reproductive Mechanisms in the Male Germ Cell
Biological Sciences, Faculty of Science and Information Technology, The University of Newcastle
Co-Supervisor
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Dr Mark Baker

Position

Research Fellow
School of Environmental and Life Sciences
Faculty of Science and Information Technology

Focus area

Biological Sciences

Contact Details

Email mark.baker@newcastle.edu.au
Phone (02) 4921 7880
Fax (02) 4921 6308

Office

Room LS4-42
Building Life Sciences
Location Callaghan
University Drive
Callaghan, NSW 2308
Australia
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