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Dr Mark Baker

Research Fellow

School of Environmental and Life Sciences (Biological Sciences)

Kicking goals

Dr Mark Baker has picked up the pace of sperm cell research by harnessing the power of proteomics.

Mark Baker on the soccer field 

There is a competitive side to Dr Mark Baker, who likes to keep fit and, at 37, still enjoys the challenge of matching strides with younger opponents on the soccer field. But the race Baker is most keen to win is the one to develop a male version of the contraceptive pill – and the proteomics expert believes his research team at the University of Newcastle has a real chance of achieving that goal.

"We are the world leaders in sperm proteomics," points out Baker, who was the first researcher to create a complete inventory of the proteins in sperm and has established a state-of-the-art proteomics laboratory at the University. "The work we do is part of a worldwide effort but we are recognised as pioneers and the most progressive group in our field."

Baker joined the University's influential Reproductive Science Group, led by Laureate Professor John Aitken, after being awarded a PhD from Monash University in 2002. Aitken, who specialises in sperm cell biology, recognised the potential in combining their areas of expertise towards the common goal of solving the mysteries of male fertility. 

Whilst Baker's ultimate goal is to develop a male contraceptive, his research has equal potential to assist men who are infertile, as it is necessary to understand the mechanisms of infertility in order to mimic the condition with a male contraceptive. In identifying and publishing the sperm proteome, Baker advanced the number of known proteins from less than 40 to more than 1200. He and the research team have subsequently narrowed down that list of proteins to a handful that can potentially be targeted by fertility drugs.

"One of the important criteria is that the protein is found only in the testes, because if you are designing a drug that inhibits a protein you don't want it to affect other parts of the body," Baker explains.  "So, we have identified a number of proteins that we know are both unique to the testes and are vital for fertility. We are now moving into the next phase of the research, which is designing a drug to target them."

Baker's work has attracted the attention of Bayer (formerly Bayer Schering Pharma), one of the first companies to market the contraceptive pill back in the 1960s. He has also established collaborations with leading international research groups in the field.

"The implications of our research are widespread when you consider that about half of all pregnancies in Australia are unwanted or unplanned and that there are about 250 abortions for every 1000 live births," Baker asserts. "There are also thousands of women who suffer side effects from contraception and there is an inextricable link between poverty and high birth rates in developing counties. As you can imagine, there is a real global imperative to improve options by developing a male contraceptive. At the same time, up to 10 per cent of the male population is infertile, so we want to be able to help them as well."

Baker has contributed his expertise in identifying the protein structure of cells to other interesting collaborations with leading University health researchers. He is currently working with Professor Hubert Hondermark on a project to detect metastatic potential in breast cancer cells and with Conjoint Professor Jim Denham to determine new biomarkers for prostate cancer. He is also working with members of the translational stroke research team on a project investigating indicators of stroke in cerebral spinal fluid.

Baker holds a National Health and Medical Research Council (NHMRC) Career Development Fellowship to research sperm motility and is an investigator on several projects supported by the NHMRC. Late last year he received the 2011 Early Career Researcher Award sponsored by the Hunter Medical Research Institute (HMRI) fundraising group PULSE.

Baker's research in sperm-egg recognition and proteomic analysis of sperm cells is internationally recognised and has been cited more than 950 times. The proteomics laboratory he was instrumental  in establishing, with its world-class mass spectrometry equipment, has made a broad contribution to research at the University of Newcastle,  supporting work across the disciplines of life science, science, medicine and engineering as well as research from HMRI and international and national collaborations.

Two minds are better than one

The powerful combination of Laureate Professor John Aitken's vast knowledge of sperm cell biology and Dr Mark Baker's proteomics prowess makes the University of Newcastle's Reproductive Science Group a formidable force in the research field of sperm function and male fertility.

"John and I decided seven years ago that we needed to investigate the proteins in sperm cells to understand the mechanisms of fertility," Baker advises. "We saw the potential in it and went after it, and the University has backed us all the way. We quickly got into the field and we have raised the bar."

Baker believes he has benefited greatly from working with Aitken, one of the world's most esteemed reproductive scientists. "He has been my primary mentor, but it is a relationship built on mutual respect," Baker emphasises. "There is a willingness to work together but also a willingness to listen to each other's points of view – and that is critical to successful research: to be able to agree but also disagree."

Aitken describes Baker as"an ideal example of how postdoctoral researchers can blossom into fully-fledged professional scientists when the University supports their careers."Now, Mark is generating research income in his own right that will more than repay the investment the University has made in developing his research portfolio."

Visit the Centre for Reproductive Science website

Visit the HMRI website

Mark Baker on the soccer field

Kicking goals

Dr Mark Baker has picked up the pace of sperm cell research by harnessing the power of proteomics.

Read more

Career Summary

Biography

After obtaining an Australia postgraduate Award, I did my PhD at Monash University, Department of Biochemistry and Cell Biology under the supervision of Dr. Alfons Lawen. Following a successful time, I was invited to the join the group at the University of Newcastle under Prof. John Aitken, which was a continuation of my PhD theme in the identifying plasma membrane redox systems. Within two years, I decided to set-up and establish a proteomics platform at the University of Newcastle and have since been successful in establishing 4 mass spectrometers in the Advanced mass spectrometry facility. In 2005 I was awarded the university of Newcastle Fellowship (rank #1 from 96 applications) which allowed 5 years of full time research. In 2011 I was awarded the NHMRC career development fellowship (level 2) from the NHMRC. I have been successful on 3 NHMRC DP grants, 1 ARC project grant and 3 ARC LIEF grants.

Research Expertise

Proteomics and phospho-proteomics comparisons using label-free mass spectrometry.

Teaching Expertise
6 lectures to Biol 3020 on reproductive physiology.

Administrative Expertise
Grants, animal ethics, Health and Safety.

Collaborations
Nuch Tanphaichitr, Senior Scientist, Ottawa Hospital Research Institute, and Professor in Obstetrics and Gynecology, and Biochemistry/Microbiology/Immunology, University of Ottawa Harvey Florman, University of Massachusetts Melissa Junganickel, University of Massachusetts Gibbes Johnson, Acting Deputy Director, Division of Therapeutic Proteins CDER, FDA, Bethesda University John Aitken, University of Newcastle Neil Sprat, University of Newcastle Tony Velkov, University of Newcastle Brett Nixon, University of Newcastle


Qualifications

  • PhD, Monash University

Keywords

  • Biol 3000
  • Fertilization
  • Phosphorylation
  • Spermatozoa

Fields of Research

CodeDescriptionPercentage
060111Signal Transduction40
060199Biochemistry and Cell Biology not elsewhere classified30
111499Paediatrics and Reproductive Medicine not elsewhere classified30

Professional Experience

UON Appointment

DatesTitleOrganisation / Department
28/02/2015 - 27/02/2017Research FellowUniversity of Newcastle
School of Environmental and Life Sciences
Australia

Academic appointment

DatesTitleOrganisation / Department
1/02/2012 - 1/12/2015Fellow - NHMRCUniversity of Newcastle
School of Environmental and Life Sciences
Australia
1/01/2010 - NHMRC Career Development FellowUniversity of Newcastle
School of Environmental and Life Sciences
Australia
1/06/2007 - 1/02/2012Fellow - UONUniversity of Newcastle
School of Environmental and Life Sciences
Australia
1/01/2005 - 1/01/2011University of Newcastle Research FellowUniversity of Newcastle
School of Environmental and Life Sciences
Australia
1/01/2002 - 1/01/2005Post docUniversity of Newcastle
School of Elect Engineering and Computer Science
Australia
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Publications

For publications that are currently unpublished or in-press, details are shown in italics.


Chapter (5 outputs)

YearCitationAltmetricsLink
2013Baker MA, Aitken RJ, 'Mass Spectrometry proteomics and the study of sperm cell biology', Human Gametes and Preimplantation Embryos, Springer, New York, NY 193-203 (2013) [B1]
DOI10.1007/978-1-4614-6651-2_18
Co-authorsJohn Aitken
2013Baker MA, 'Phosphoproteomic analysis of peptides', 271-277 (2013) [B2]

Posttranslational modifications represent a major mechanism by which cells control molecular events. Sample preparation is one of the biggest keys in terms of proteomic analysis, ... [more]

Posttranslational modifications represent a major mechanism by which cells control molecular events. Sample preparation is one of the biggest keys in terms of proteomic analysis, and especially when looking at phosphoproteomics. Here we describe two techniques that are used to identify phosphopeptides. These are Titanium Dioxide and Immobilized Metal Affinity Chromatography (IMAC) which enrich for phosphopeptides. These enrichment techniques typically often identify ~35 % of the same and ~65 % unique phosphopeptides. In this chapter, we describe the use of an ion-trap mass spectrometer for the identification of phosphopeptides. © 2013 Springer Science+Business Media, LLC.

DOI10.1007/978-1-62703-613-9_18
2012Aitken RJ, De Iuliis GN, Baker MA, 'Direct methods for the detection of reactive oxygen species in human semen samples', Studies on Men's Health and Fertility, Springer, New York 275-299 (2012) [B1]
Co-authorsJohn Aitken, Geoffry DeiuliIs
2010Aitken RJ, Baker MA, De Iuliis GN, Nixon B, 'New insights into sperm physiology and pathology', Fertility Control, Springer, Berlin 99-115 (2010) [B1]
CitationsScopus - 23
Co-authorsGeoffry DeiuliIs, Brett Nixon, John Aitken
2007Aitken RJ, Roman SD, Baker MA, De Iuliis GN, 'Redox regulation of DNA damage in the male germ line', Male-Mediated Developmental Toxicity, Royal Society of Chemistry, London, United Kingdom 197-209 (2007) [B1]
CitationsWeb of Science - 2
Co-authorsShaun Roman, Geoffry DeiuliIs, John Aitken
Show 2 more chapters

Journal article (72 outputs)

YearCitationAltmetricsLink
2015Kongmanas K, Kruevaisayawan H, Saewu A, Sugeng C, Fernandes J, Souda P, et al., 'Proteomic Characterization of Pig Sperm Anterior Head Plasma Membrane Reveals Roles of Acrosomal Proteins in ZP3 Binding', JOURNAL OF CELLULAR PHYSIOLOGY, 230 449-463 (2015)
DOI10.1002/jcp.24728Author URL
CitationsScopus - 1Web of Science - 1
Co-authorsJohn Aitken
2015Baker MA, Weinberg A, Hetherington L, Villaverde AI, Velkov T, 'Analysis of protein thiol changes occurring during rat sperm epididymal maturation.', Biol Reprod, 92 11 (2015)
DOI10.1095/biolreprod.114.123679Author URL
2015Young SA, Aitken J, Baker MA, 'Phosphorylation of Izumo1 and Its Role in Male Infertility.', Asian J Androl, (2015)
Author URL
2015Schneider EK, Huang JX, Carbone V, Baker M, Azad MAK, Cooper MA, et al., 'Drug-drug plasma protein binding interactions of ivacaftor', JOURNAL OF MOLECULAR RECOGNITION, 28 339-348 (2015)
DOI10.1002/jmr.2447Author URL
2015Aitken RJ, Baker MA, Nixon B, 'Are sperm capacitation and apoptosis the opposite ends of a continuum driven by oxidative stress?', Asian J Androl, 17 633-639 (2015)
DOI10.4103/1008-682X.153850Author URL
Co-authorsBrett Nixon, John Aitken
2015Baker MA, Weinberg A, Hetherington L, Villaverde AI, Velkov T, Baell J, Gordon CP, 'Defining the mechanisms by which the reactive oxygen species by-product, 4-hydroxynonenal, affects human sperm cell function.', Biol Reprod, 92 108 (2015)
DOI10.1095/biolreprod.114.126680Author URL
CitationsWeb of Science - 1
2014Baker MA, Hetherington L, Weinberg A, Velkov T, 'Phosphopeptide analysis of rodent epididymal spermatozoa', Journal of visualized experiments : JoVE, (2014) [C1]

Spermatozoa are quite unique amongst cell types. Although produced in the testis, both nuclear gene transcription and translation are switched off once the pre-cursor round cell b... [more]

Spermatozoa are quite unique amongst cell types. Although produced in the testis, both nuclear gene transcription and translation are switched off once the pre-cursor round cell begins to elongate and differentiate into what is morphologically recognized as a spermatozoon. However, the spermatozoon is very immature, having no ability for motility or egg recognition. Both of these events occur once the spermatozoa transit a secondary organ known as the epididymis. During the ~12 day passage that it takes for a sperm cell to pass through the epididymis, post-translational modifications of existing proteins play a pivotal role in the maturation of the cell. One major facet of such is protein phosphorylation. In order to characterize phosphorylation events taking place during sperm maturation, both pure sperm cell populations and pre-fractionation of phosphopeptides must be established. Using back flushing techniques, a method for the isolation of pure spermatozoa of high quality and yield from the distal or caudal epididymides is outlined. The steps for solubilization, digestion, and pre-fractionation of sperm phosphopeptides through TiO2 affinity chromatography are explained. Once isolated, phosphopeptides can be injected into MS to identify both protein phosphorylation events on specific amino acid residues and quantify the levels of phosphorylation taking place during the sperm maturation processes.

DOI10.3791/51546
2014Aitken RJ, Smith TB, Jobling MS, Baker MA, De Iuliis GN, 'Oxidative stress and male reproductive health', Asian Journal of Andrology, 16 31-38 (2014) [C1]

One of the major causes of defective sperm function is oxidative stress, which not only disrupts the integrity of sperm DNA but also limits the fertilizing potential of these cell... [more]

One of the major causes of defective sperm function is oxidative stress, which not only disrupts the integrity of sperm DNA but also limits the fertilizing potential of these cells as a result of collateral damage to proteins and lipids in the sperm plasma membrane. The origins of such oxidative stress appear to involve the sperm mitochondria, which have a tendency to generate high levels of superoxide anion as a prelude to entering the intrinsic apoptotic cascade. Unfortunately, these cells have very little capacity to respond to such an attack because they only possess the first enzyme in the base excision repair (BER) pathway, 8-oxoguanine glycosylase 1 (OGG1). The latter successfully creates an abasic site, but the spermatozoa cannot process the oxidative lesion further because they lack the downstream proteins (APE1, XRCC1) needed to complete the repair process. It is the responsibility of the oocyte to continue the BER pathway prior to initiation of S-phase of the first mitotic division. If a mistake is made by the oocyte at this stage of development, a mutation will be created that will be represented in every cell in the body. Such mechanisms may explain the increase in childhood cancers and other diseases observed in the offspring of males who have suffered oxidative stress in their germ line as a consequence of age, environmental or lifestyle factors. The high prevalence of oxidative DNA damage in the spermatozoa of male infertility patients may have implications for the health of children conceivedin vitro and serves as a driver for current research into the origins of free radical generation in the germ line. © 2014 AJA, SIMM & SJTU. All rights reserved.

DOI10.4103/1008-682X.122203
CitationsScopus - 19Web of Science - 19
Co-authorsJohn Aitken, Geoffry DeiuliIs
2014Velkov T, Deris ZZ, Huang JX, Azad MAK, Butler M, Sivanesan S, et al., 'Surface changes and polymyxin interactions with a resistant strain of Klebsiella pneumoniae', INNATE IMMUNITY, 20 350-363 (2014) [C1]
DOI10.1177/1753425913493337Author URL
CitationsScopus - 2Web of Science - 3
2014Baker MA, Hetherington L, Weinberg A, Velkov T, 'Phosphopeptide analysis of rodent epididymal spermatozoa', Journal of visualized experiments : JoVE, (2014) [C1]

Spermatozoa are quite unique amongst cell types. Although produced in the testis, both nuclear gene transcription and translation are switched off once the pre-cursor round cell b... [more]

Spermatozoa are quite unique amongst cell types. Although produced in the testis, both nuclear gene transcription and translation are switched off once the pre-cursor round cell begins to elongate and differentiate into what is morphologically recognized as a spermatozoon. However, the spermatozoon is very immature, having no ability for motility or egg recognition. Both of these events occur once the spermatozoa transit a secondary organ known as the epididymis. During the ~12 day passage that it takes for a sperm cell to pass through the epididymis, post-translational modifications of existing proteins play a pivotal role in the maturation of the cell. One major facet of such is protein phosphorylation. In order to characterize phosphorylation events taking place during sperm maturation, both pure sperm cell populations and pre-fractionation of phosphopeptides must be established. Using back flushing techniques, a method for the isolation of pure spermatozoa of high quality and yield from the distal or caudal epididymides is outlined. The steps for solubilization, digestion, and pre-fractionation of sperm phosphopeptides through TiO2 affinity chromatography are explained. Once isolated, phosphopeptides can be injected into MS to identify both protein phosphorylation events on specific amino acid residues and quantify the levels of phosphorylation taking place during the sperm maturation processes.

DOI10.3791/51546
2014Arjomand A, Baker MA, Li C, Buckle AM, Jans DA, Loveland KL, Miyamoto Y, 'The a-importome of mammalian germ cell maturation provides novel insights for importin biology.', FASEB J, 28 3480-3493 (2014) [C1]
DOI10.1096/fj.13-244913Author URL
CitationsScopus - 3Web of Science - 3
2014Baker MA, Hetherington L, Weinberg A, Velkov T, 'Phosphopeptide Analysis of Rodent Epididymal Spermatozoa', JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, (2014) [C1]
DOI10.3791/51546Author URL
2013Carbone V, Kim H, Huang JX, Baker MA, Ong C, Cooper MA, et al., 'Molecular characterization of the receptor binding structure-activity relationships of influenza B virus hemagglutinin', Acta Virologica: international journal, 57 313-332 (2013) [C1]
DOI10.4149/av_2013_03_313Author URL
CitationsScopus - 1
2013Velkov T, Ong C, Baker MA, Kim H, Li J, Nation RL, et al., 'The antigenic architecture of the hemagglutinin of influenza H5N1 viruses', Molecular Immunology, 56 705-719 (2013) [C1]
DOI10.1016/j.molimm.2013.07.010Author URL
CitationsScopus - 8Web of Science - 8
2013Rockman S, Camuglia S, Vandenberg K, Ong C, Baker MA, Nation RL, et al., 'Reverse engineering the antigenic architecture of the haemagglutinin from influenza H5N1 clade 1 and 2.2 viruses with fine epitope mapping using monoclonal antibodies', Molecular Immunology, 53 435-442 (2013) [C1]
DOI10.1016/j.molimm.2012.10.001Author URL
CitationsScopus - 4Web of Science - 2
2013Aitken RJ, Baker MA, 'Oxidative stress, spermatozoa and leukocytic infiltration: Relationships forged by the opposing forces of microbial invasion and the search for perfection', Journal of Reproductive Immunology, 100 11-19 (2013) [C1]
DOI10.1016/j.jri.2013.06.005
CitationsScopus - 6Web of Science - 5
Co-authorsJohn Aitken
2013Miyamoto Y, Baker MA, Whiley PA, Arjomand A, Ludeman J, Wong C, et al., 'Towards delineation of a developmental alpha-importome in the mammalian male germline', BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH, 1833 731-742 (2013) [C1]
DOI10.1016/j.bbamcr.2012.11.005Author URL
CitationsScopus - 6Web of Science - 6
2013Aitken RJ, Baker MA, 'Causes and consequences of apoptosis in spermatozoa; contributions to infertility and impacts on development', International Journal of Developmental Biology, 57 265-272 (2013) [C1]

During early development, apoptosis plays a major role in the ontogeny of the germ line as a means of regulating the germ cell:Sertoli cell ratio. In the adult, apoptosis fulfils ... [more]

During early development, apoptosis plays a major role in the ontogeny of the germ line as a means of regulating the germ cell:Sertoli cell ratio. In the adult, apoptosis fulfils another function in removing damaged germ cells from the seminiferous epithelium in response to a wide range of physiological and environmental triggers. These include various forms of electromagnetic radiation, chemotherapeutic agents and commonly encountered toxicants such as phthalate es-ters, bisphenol A and cadmium. This form of apoptosis can lead to spermatogenic arrest and is predominantly mediated by the Fas/FasL system. In addition, senescent mature spermatozoa can undergo a truncated form of apoptosis in order to ensure their efficient phagocytosis within the male and female reproductive tracts. This apoptotic cascade appears to be triggered by oxidative stress and lipid peroxidation, which leads to activation of mitochondrial reactive oxygen species (ROS) generation in a self-perpetuating redox cycle. The electrophilic aldehydes generated as a result of lipid peroxidation also lead to a rapid loss of sperm motility followed some hours later by caspase activation and phosphatidylserine exposure on the sperm surface. The nuclear DNA suffers oxidative damage during this process but there is no immediate DNA cleavage by endonucleases as there is in somatic cells. The reasons for this deviation from the normal pattern of apoptosis involve the unusual physical architecture of spermatozoa and the limited capacity these cells possess for base-excision repair. These findings have practical implications for the approaches that might be used to detect and prevent DNA damage in spermatozoa. © 2013 UBC Press.

DOI10.1387/ijdb.130146ja
CitationsScopus - 13Web of Science - 12
Co-authorsJohn Aitken
2013Smith TB, Baker MA, Connaughton HS, Habenicht U, Aitken RJ, 'Functional deletion of Txndc2 and Txndc3 increases the susceptibility of spermatozoa to age-related oxidative stress', Free Radical Biology and Medicine, 65 872-881 (2013) [C1]
DOI10.1016/j.freeradbiomed.2013.05.021Author URL
CitationsScopus - 6Web of Science - 4
Co-authorsJohn Aitken
2013Baker MA, Weinberg AS, Hetherington L, Velkov T, Aitken RJ, 'Post-ejaculatory changes in the metabolic status of rat spermatozoa as measured by GC-MS', Metabolomics, 9 708-721 (2013) [C1]
DOI10.1007/s11306-012-0478-5Author URL
Co-authorsJohn Aitken
2013Baker MA, Naumovski N, Hetherington L, Weinberg A, Velkov T, Aitken RJ, 'Head and flagella subcompartmental proteomic analysis of human spermatozoa', Proteomics, 13 61-74 (2013) [C1]
DOI10.1002/pmic.201200350Author URL
CitationsScopus - 29Web of Science - 29
Co-authorsJohn Aitken
2013Velkov T, Soon RL, Chong PL, Huang JX, Cooper MA, Azad MAK, et al., 'Molecular basis for the increased polymyxin susceptibility of Klebsiella pneumoniae strains with under-acylated lipid A', Innate Immunity, 19 265-277 (2013) [C1]
DOI10.1177/1753425912459092Author URL
CitationsScopus - 3Web of Science - 3
2013Aitken RJ, Smith TB, Lord T, Kuczera L, Koppers AJ, Naumovski N, et al., 'On methods for the detection of reactive oxygen species generation by human spermatozoa: analysis of the cellular responses to catechol oestrogen, lipid aldehyde, menadione and arachidonic acid', Andrology, 1 192-205 (2013) [C1]
DOI10.1111/j.2047-2927.2012.00056.xAuthor URL
CitationsScopus - 11Web of Science - 11
Co-authorsJohn Aitken, Geoffry DeiuliIs
2012Aitken RJ, Whiting SJ, De Iuliis GN, McClymont SL, Mitchell LA, Baker MA, 'Electrophilic aldehydes generated by sperm metabolism activate mitochondrial reactive oxygen species generation and apoptosis by targeting succinate dehydrogenase', Journal of Biological Chemistry, 287 33048-33060 (2012) [C1]
DOI10.1074/jbc.M112.366690
CitationsScopus - 29Web of Science - 26
Co-authorsGeoffry DeiuliIs, John Aitken
2012Aitken RJ, De Iuliis GN, Gibb Z, Baker MA, 'The Simmet lecture: New horizons on an old landscape - oxidative stress, DNA damage and apoptosis in the male germ line', Reproduction in Domestic Animals, 47 7-14 (2012) [C2]
CitationsScopus - 10Web of Science - 10
Co-authorsGeoffry DeiuliIs, John Aitken
2012Huang JX, Cooper MA, Baker MA, Azad MAK, Nation RL, Li J, Velkov T, 'Drug-binding energetics of human alpha-1-acid glycoprotein assessed by isothermal titration calorimetry and molecular docking simulations', Journal of Molecular Recognition, 25 642-656 (2012) [C1]
CitationsScopus - 3Web of Science - 3
2012Baker MA, Hetherington L, Weinberg AS, Naumovski N, Velkov T, Pelzing M, et al., 'Analysis of phosphopeptide changes as spermatozoa acquire functional competence in the epididymis demonstrates changes in the post-translational modification of Izumo1', Journal of Proteome Research, 11 5252-5264 (2012) [C1]
DOI10.1021/pr300468m
CitationsScopus - 5Web of Science - 5
Co-authorsJohn Aitken
2012Redgrove KA, Nixon B, Baker MA, Hetherington L, Baker G, Liu D-Y, Aitken RJ, 'The molecular chaperone HSPA2 plays a key role in regulating the expression of sperm surface receptors that mediate sperm-egg recognition', Plos One, 7 1-16 (2012) [C1]
CitationsScopus - 20Web of Science - 19
Co-authorsJohn Aitken, Brett Nixon
2012Baker MA, Nixon B, Naumovski N, Aitken RJ, 'Proteomic insights into the maturation and capacitation of mammalian spermatozoa', Systems Biology in Reproductive Medicine, 58 211-217 (2012) [C1]
DOI10.3109/19396368.2011.639844
CitationsScopus - 22Web of Science - 20
Co-authorsJohn Aitken, Brett Nixon
2012Huang JX, Azad MAK, Yuriev E, Baker MA, Nation RL, Li J, et al., 'Molecular characterization of lipopolysaccharide binding to human a-1-acid glycoprotein', Journal of Lipids, - 1-15 (2012) [C1]
2011Baker MA, Smith ND, Hetherington L, Pelzing M, Condina MR, Aitken RJ, 'Use of titanium dioxide to find phosphopeptide and total protein changes during epididymal sperm maturation', Journal of Proteome Research, 10 1004-1017 (2011) [C1]
DOI10.1021/pr1007224
CitationsScopus - 21Web of Science - 17
Co-authorsJohn Aitken
2011Dun MD, Smith ND, Baker MA, Lin M, Aitken RJ, Nixon B, 'The chaperonin containing TCP1 complex (CCT/TRiC) is involved in mediating sperm-oocyte interaction', Journal of Biological Chemistry, 286 36875-36887 (2011) [C1]
DOI10.1074/jbc.m110.188888
CitationsScopus - 19Web of Science - 17
Co-authorsMatt Dun, Minjie Lin, Brett Nixon, John Aitken
2011Baker MA, 'The 'omics revolution and our understanding of sperm cell biology', Asian Journal of Andrology, 13 6-10 (2011) [C1]
DOI10.1038/aja.2010.62
CitationsScopus - 21Web of Science - 23
2011Aitken RJ, Baker MA, 'Cell biology of human spermatozoa: Lessons from the Proteome', Australian Biochemist, 42 12-15 (2011) [C3]
Co-authorsJohn Aitken
2010Baker MA, Smith ND, Hetherington L, Taubman K, Graham ME, Robinson PJ, Aitken RJ, 'Label-free quantitation of phosphopeptide changes during rat sperm capacitation', Journal of Proteome Research, 9 718-729 (2010) [C1]
DOI10.1021/pr900513d
CitationsScopus - 25Web of Science - 19
Co-authorsJohn Aitken
2010Baker MA, Reeves GM, Hetherington L, Aitken RJ, 'Analysis of proteomic changes associated with sperm capacitation through the combined use of IPG-strip pre-fractionation followed by RP chromatography LC-MS/MS analysis', Proteomics, 10 482-495 (2010) [C1]
DOI10.1002/pmic.200900574
CitationsScopus - 34Web of Science - 34
Co-authorsJohn Aitken
2009Baker MA, Aitken RJ, 'Proteomic insights into spermatozoa: Critiques, comments and concerns', Expert Review of Proteomics, 6 691-705 (2009) [C1]
DOI10.1586/epr.09.76
CitationsScopus - 29Web of Science - 26
Co-authorsJohn Aitken
2009Ewen KA, Baker MA, Wilhelm D, Aitken RJ, Koopman P, 'Global survey of protein expression during gonadal sex determination in mice', Molecular & Cellular Proteomics, 8 2624-2641 (2009) [C1]
DOI10.1074/mcp.m900108-mcp200
CitationsScopus - 8Web of Science - 7
Co-authorsJohn Aitken
2009Baker MA, Hetherington L, Curry BJ, Aitken RJ, 'Phosphorylation and consequent stimulation of the tyrosine kinase c-Abl by PKA in mouse spermatozoa: Its implications during capacitation', Developmental Biology, 333 57-66 (2009) [C1]
DOI10.1016/j.ydbio.2009.06.022
CitationsScopus - 25Web of Science - 24
Co-authorsBen Curry, John Aitken
2008Mitchell LA, Nixon B, Baker MA, Aitken RJ, 'Investigation of the role of SRC in capacitation-associated tyrosine phosphorylation of human spermatozoa', Molecular Human Reproduction, 14 235-243 (2008) [C1]
DOI10.1093/molehr/gan007
CitationsScopus - 46Web of Science - 44
Co-authorsJohn Aitken, Brett Nixon
2008Baker MA, Hetherington L, Reeves GM, Muller J, Aitken RJ, 'The rat sperm proteome characterized via IPG strip prefractionation and LC-MS/MS identification', Proteomics, 8 2312-2321 (2008) [C1]
DOI10.1002/pmic.200700876
CitationsScopus - 88Web of Science - 64
Co-authorsJohn Aitken
2008Baker MA, Hetherington L, Reeves GM, Aitken RJ, 'The mouse sperm proteome characterized via IPG strip prefractionation and LC-MS/MS identification', Proteomics, 8 1720-1730 (2008) [C1]
DOI10.1002/pmic.200701020
CitationsScopus - 10Web of Science - 76
Co-authorsJohn Aitken
2008Aitken RJ, Hughes LM, Griffith R, Baker MA, 'Bridging the gap between male and female fertility control; contraception-on-demand', Contraception, 78 S28-S35 (2008) [C1]
DOI10.1016/j.contraception.2008.03.020
CitationsScopus - 8Web of Science - 4
Co-authorsJohn Aitken
2008Aitken RJ, Baker MA, Doncel GF, Matzuk MM, Mauck CK, Harper MJK, 'As the world grows: Contraception in the 21st century', Journal of Clinical Investigation, 118 1330-1343 (2008) [C1]
DOI10.1172/jci33873
CitationsScopus - 40Web of Science - 28
Co-authorsJohn Aitken
2008Aitken RJ, Baker MA, 'The role of proteomics in understanding sperm cell biology', International Journal of Andrology, 31 295-302 (2008) [C1]
DOI10.1111/j.1365-2605.2007.00851.x
CitationsScopus - 61Web of Science - 59
Co-authorsJohn Aitken
2007Aitken RJ, Nixon B, Lin M, Koppers AJ, Lee YH, Baker MA, 'Proteomic changes in mammalian spermatozoa during epididymal maturation', Asian Journal of Andrology, 9 554-564 (2007) [C1]
DOI10.1111/j.1745-7262.2007.00280.x
CitationsScopus - 70Web of Science - 62
Co-authorsJohn Aitken, Brett Nixon, Minjie Lin
2007Baker MA, Reeves GE, Hetherington L, Muller J, Baur I, Aitken RJ, 'Identification of gene products present in Triton X-100 soluble and insoluble fractions of human spermatozoa lysates using LC-MS/MS analysis', Proteomics Clinical Applications, 1 524-532 (2007) [C1]
DOI10.1002/prca.200601013
CitationsScopus - 98Web of Science - 93
Co-authorsJohn Aitken
2006Baker MA, Hetherington L, Aitken RJ, 'Identification of SRC as a key PKA-stimulated tyrosine kinase involved in the capacitation-associated hyperactivation of murine spermatozoa', Journal of Cell Science, 119 3182-3192 (2006) [C1]
DOI10.1242/jcs.03055
CitationsScopus - 99Web of Science - 98
Co-authorsJohn Aitken
2006Aitken RJ, Baker MA, 'Oxidative stress, sperm survival and fertility control', Molecular and Cellular Endocrinology, 250 66-69 (2006) [C1]
DOI10.1016/j.mce.2005.12.026
CitationsScopus - 177Web of Science - 158
Co-authorsJohn Aitken
2006Lin M, Lee YH, Xu W, Baker MA, Aitken RJ, 'Ontogeny of tyrosine phosphorylation-signaling pathways during spermatogenesis and epididymal maturation in the mouse', Biology of Reproduction, 75 588-597 (2006) [C1]
DOI10.1095/biolreprod.106.052712
CitationsScopus - 16Web of Science - 15
Co-authorsMinjie Lin, John Aitken
2005Baker MA, Aitken RJ, 'Reactive oxygen species in spermatozoa: methods for monitoring and significance for the origins of genetic disease and infertility', Reproductive Biology and Endocrinology, 3 9 (2005) [C1]
DOI10.1186/1477-7827-3-67
CitationsScopus - 95Web of Science - 82
Co-authorsJohn Aitken
2005Baker MA, Krutskikh A, Curry BJ, Hetherington L, Aitken RJ, 'Identification of cytochrome-b5 reductase as the enzyme responsible for NADH-dependent lucigenin chemiluminescence in human spermatozoa', Biology of Reproduction, 73 334-342 (2005) [C1]
DOI10.1095/biolreprod.104.037960
CitationsScopus - 25Web of Science - 25
Co-authorsBen Curry, John Aitken
2005Baker MA, Witherdin RF, Hetherington L, Cunningham KA, Aitken RJ, 'Identification of post-translational modifications that occur during sperm maturation using difference in two-dimensional gel electrophoresis', Proteomics, 5 1003-1012 (2005) [C1]
DOI10.1002/pmic.200401100
CitationsScopus - 71Web of Science - 68
Co-authorsJohn Aitken
2004Baker MA, Attard B, Aitken RJ, 'TWO- DIMENSIONAL DIFFERENTIAL IN GEL ELECTROPHORESIS ( A NOVEL METHOD FOR HIGH THROUGHPUT PROTEOMICS', Australian Biochemist, 35 40-42 (2004)
2004Baker MA, Krutskikh A, Curry BJ, McLaughlin EA, Aitken RJ, 'Identification of Cytochrome P450-Reductase as the Enzyme Responsible for NADPH-Dependent Lucigenin and Tetrazolium Salt Reduction in Rat Epididymal Sperm Preparations', Biology of Reproduction, 71 307-318 (2004) [C1]
DOI10.1095/biolreprod.104.027748
CitationsScopus - 35Web of Science - 34
Co-authorsJohn Aitken, Eileen Mclaughlin, Ben Curry
2004Baker MA, Lane DJR, Ly JD, De Pinto V, Lawen A, 'VDAC1 Is a Transplasma Membrane NADH-Ferricyanide Reductase', Journal of Biological Chemistry, 279 4811-4819 (2004)

Porin isoform 1 or VDAC (voltage-dependent anion-selective channel) 1 is the predominant protein in the outer mitochondrial membrane. We demonstrated previously that a plasma memb... [more]

Porin isoform 1 or VDAC (voltage-dependent anion-selective channel) 1 is the predominant protein in the outer mitochondrial membrane. We demonstrated previously that a plasma membrane NADH-ferricyanide reductase activity becomes up-regulated upon mitochondrial perturbation, and therefore suggested that it functions as a cellular redox sensor. VDAC1 is known to be expressed in the plasma membrane; however, its function there remained a mystery. Here we show that VDAC1, when expressed in the plasma membrane, functions as a NADH-ferricyanide reductase. VDAC1 preparations purified from both plasma membrane and mitochondria fractions exhibit NADH-ferricyanide reductase activity, which can be immunoprecipitated with poly- and monoclonal antibodies directed against VDAC(1). Transfecting cells with pl-VDAC1. GFP, which carries an N-terminal signal peptide, directs VDAC1 to the plasma membrane, as shown by confocal microscopy and FACS analysis, and significantly increases the plasma membrane NADH-ferricyanide reductase activity of the transfected cells. This novel enzymatic activity of the well known VDAC1 molecule may provide an explanation for its role in the plasma membrane. Our data suggest that a major function of VDAC1 in the plasma membrane is that of a NADH(-ferricyanide) reductase that may be involved in the maintenance of cellular redox homeostasis.

DOI10.1074/jbc.M311020200
CitationsScopus - 124Web of Science - 112
2004Baker MA, Hetherington L, Ecroyd HW, Roman SD, Aitken RJ, 'Analysis of the mechanism by which calcium negatively regulates the tyrosine phoshorylation cascade associated with sperm capacitation', Journal of Cell Science, 117 211-222 (2004) [C1]
DOI10.1242/jcs.00842
Co-authorsJohn Aitken, Shaun Roman
2004Aitken RJ, Baker MA, O'Bryan M, 'Shedding Light on Chemiluminescence: The Application of Chemiluminescence in Diagnostic Andrology', Journal of Andrology, 25 455-465 (2004) [C1]
CitationsScopus - 31Web of Science - 34
Co-authorsJohn Aitken
2004Baker MA, Aitken RJ, 'The importance of redox regulated pathways in sperm cell biology', Molecular and Cellular Endocrinology, 216 47-54 (2004) [C1]
DOI10.1016/j.mce.2003.10.068
CitationsScopus - 90Web of Science - 83
Co-authorsJohn Aitken
2004Aitken RJ, Ryan AL, Baker MA, McLaughlin EA, 'Redox activity associated with the maturation and capacitation of mammalian spermatozoa', Free Radical Biology and Medicine, 36 994-1010 (2004) [C1]
DOI10.1016/j.freeradbiomed.2004.01.017
CitationsScopus - 73Web of Science - 64
Co-authorsEileen Mclaughlin, John Aitken
2004Baker MA, Ly JD, Lawen A, 'Characterization of VDAC1 as a plasma membrane NADH-oxidoreductase', BIOFACTORS, 21 215-221 (2004)
Author URL
CitationsScopus - 15Web of Science - 13
2004Aitken RJ, Baker MA, 'Oxidative stress and male reproductive biology', Reproduction, Fertility and Development, 16 581-588 (2004) [C1]
DOI10.1071/RD03089
CitationsScopus - 130Web of Science - 121
Co-authorsJohn Aitken
2004Sidhu KS, Mate KE, Gunasekera T, Veal D, Hetherington L, Baker MA, et al., 'A flow cytometric assay for global estimation of tyrosine phosphorylation associated with capacitation of spermatozoa from two marsupial species, the tammar wallaby (Macropus eugenii) and the brushtail possum (Trichosurus vulpecula)', Reproduction, 127 95-103 (2004) [C1]
DOI10.1530/rep.1.00073
CitationsScopus - 18Web of Science - 19
Co-authorsJohn Rodger, Karen Mate, John Aitken
2003Aitken RJ, Ryan AL, Curry BJ, Baker MA, 'Multiple forms of redox activity in populations of human spermatozoa', Molecular Human Reproduction, 9 645-661 (2003) [C1]
DOI10.1093/molehr/gag086
CitationsScopus - 57Web of Science - 53
Co-authorsBen Curry, John Aitken
2003Aitken RJ, Baker MA, Sawyer DE, 'Oxidative stress in the male germ line and its role in the aetiology of male infertility and genetic disease', Reproductive BioMedicine, 7 65-70 (2003) [C1]
CitationsScopus - 133
Co-authorsJohn Aitken
2003Baker MA, 'New functions of an old protein: The eukaryotic porrin or voltage dependent anion selective channel (VDAC)', Italian Journal of Biochemistry, 17-24 (2003)
2003Baker MA, Krutskikh A, Aitken RJ, 'Biochemical entities involved in reactive oxygen species generation by human spermatozoa', Protoplasma, 145-151 (2003) [C1]
DOI10.1007/s00709-002-0057-0
CitationsScopus - 25Web of Science - 24
Co-authorsJohn Aitken
2003Baker MA, Lewis B, Hetherington L, Aitken RJ, 'Development of the Signalling Pathways Associated With Sperm Capacitation During Epididymal Maturation', Molecular Reproduction and Development, 446-457 (2003) [C1]
DOI10.1002/mrd.10255
CitationsScopus - 24Web of Science - 23
Co-authorsJohn Aitken
2002Aitken RJ, Baker MA, 'Reactive oxygen species generation by human spermatozoa: a continuing enigma', International Journal of Andrology, 25 191-194 (2002) [C1]
CitationsScopus - 38Web of Science - 35
Co-authorsJohn Aitken
2002Baker MA, Grubb D, Lawen A, 'Didemnin B induces apoptosis in proliferating but not resting peripheral blood mononuclear cells', Apoptosis: an international journal on programmed cell death, 407-412 (2002) [C1]
2002Baker MA, Grubb DR, Lawen A, 'Didemnin B induces apoptosis in proliferating but not resting peripheral blood mononuclear cells', APOPTOSIS, 7 407-412 (2002) [C1]
DOI10.1023/A:1020078907108Author URL
CitationsScopus - 11Web of Science - 8
2000Baker MA, Lawen A, 'Plasma Membrane NADH-Oxidoreductase System: A Critical Review of the Structural and Functional Data', ANTIOXIDANTS & REDOX SIGNALING, 2 197-212 (2000)
DOI10.1089/ars.2000.2.2-197Author URL
CitationsScopus - 45Web of Science - 39
Show 69 more journal articles

Conference (19 outputs)

YearCitationAltmetricsLink
2013Baker M, Aitken J, 'ANALYSIS OF PHOSPHOPEPTIDE CHANGES AS SPERMATOZOA ACQUIRE FUNCTIONAL COMPETENCE IN THE EPIDIDYMIS DEMONSTRATES CHANGES IN THE POST-TRANSLATIONAL MODIFICATION OF IZUMO1', ANDROLOGY (2013) [E3]
Author URL
Co-authorsJohn Aitken
2012Nixon B, Redgrove KA, Baker MA, Aitken RJ, 'The role of molecular chaperones in regulating human sperm-egg recognition', Abstracts. The Annual Scientific Meeting of the Endocrine Society of Australia and the Society for Reproductive Biology 2012, Gold Coast, QLD (2012) [E3]
Co-authorsBrett Nixon, John Aitken
2012Aitken RJ, Nixon B, Redgrove KA, Dun M, Baker MA, 'The molecular origins of defective sperm function', HUMAN REPRODUCTION, Istanbul, TURKEY (2012) [E3]
Author URL
Co-authorsMatt Dun, John Aitken, Brett Nixon
2012Kongmanas K, Sugeng C, Souda P, Faull K, Kitajima K, Aitken RJ, et al., 'Proteomic analysis of sperm anterior head plasma membrane: Unfolding the molecular components underlying sperm-egg interaction', Reproduction in Domestic Animals, Vancouver, Canada (2012) [E3]
Co-authorsJohn Aitken
2008Baker MA, Hetherington L, Reeves GM, Smith ND, Aitken RJ, 'Comparative and phospho proteomics: Identification of protein changes during sperm maturation', Proceedings of 41st Annual Meeting of the Society for the Study of Reproduction. "Reproductive Biology: Basic Discoveries that Affect Our Lives", Kailua-Kona, Hawaii (2008) [E3]
Co-authorsJohn Aitken
2008Mitchell L, Nixon B, Baker M, Aitken R, 'Investigation of the role of SRC in capacitation associated tyrosine phosphorylation of human spermatozoa', BIOLOGY OF REPRODUCTION, Kona, HI (2008) [E3]
Author URL
Co-authorsBrett Nixon
2008Baker M, Hetherington L, Reeves G, Smith N, Aitken R, 'Comparative and phospho proteomics: Identification of protein changes during sperm maturation', BIOLOGY OF REPRODUCTION, Kona, HI (2008) [E3]
Author URL
2007Baker MA, 'The proteomic analysis of sperm maturation', Gordon Research Conferences: Fertilization & Activation of Development. Program, Plymouth, UK (2007) [E3]
2006Baker MA, Reeves GM, Aitken RJ, 'New approaches towads the proteomic analysis of the human spermatozoon', JOURNAL OF ANDROLOGY, Chicago, IL (2006)
Author URL
Co-authorsJohn Aitken
2006Aitken RJ, Baker MA, Lee YH, Lin M, Nixon B, 'Proteomic Changes in Mouse Spermatozoa During Epididymal Transit', Abstracts, Chatel-Guyon, Auvergne, France (2006) [E3]
Co-authorsBrett Nixon, John Aitken, Minjie Lin
2006Baker MA, Reeves GE, Aitken RJ, 'New Approaches Towards the Proteomic Analysis of the Human Spermatozoon', Book of Abstracts, Lorne, Victoria, Australia (2006) [E3]
Co-authorsJohn Aitken
2006Baker MA, Curry BJ, Aitken RJ, 'Inactivation of C-ABL Via PKA During Capacitation of Mouse Spermatozoa', Book of Abstracts, Lorne, Victoria, Australia (2006) [E3]
Co-authorsJohn Aitken, Ben Curry
2006Baker MA, Reeves GM, Aitken RJ, 'New approaches towards the proteomic analysis of the human spermatozoon', Book of Abstracts, Chicago, Illinois (2006) [E3]
Co-authorsJohn Aitken
2005Baker MA, Aitken RJ, 'Identification of PP60C-SRC as the enzyme responsible for tyrosine phosphorylation and consequent hyperactivation during mouse sperm capacitation', JOURNAL OF ANDROLOGY, Seattle, WA (2005)
Author URL
Co-authorsJohn Aitken
2005Baker MA, Aitken RJ, 'Identification of PP60C-SRC as the enzyme responsible for tyrosine phosphorylation and consequent hyperactivation during mouse sperm capacitation', JOURNAL OF ANDROLOGY, Seattle, WA (2005)
Author URL
Co-authorsJohn Aitken
2003Baker MA, Hetherington L, Ecroyd HW, Roman SD, Aitken RJ, 'Analysis of the mechanism by which calcium negatively regulates the tyrosine phosphorylation cascade associated with sperm capacitation', ComBio Combined Conference Abstracts, Melbourne (2003) [E3]
CitationsScopus - 63Web of Science - 56
Co-authorsShaun Roman, John Aitken
2002Baker MA, Krutskikh A, Aitken RJ, 'Redox regulation of sperm function', Proceedings of the Thirty-third annual Conference of the Society for Reproductive Biology, Adelaide, SA (2002) [E3]
Co-authorsJohn Aitken
2002Aitken RJ, Baker MA, Krutskikh A, 'Oxidative stress and redox regulation in the epididymis', Epididymis III. Third International Conference on the Epididymis., Charlottesville, Virginia, USA (2002) [E3]
Co-authorsJohn Aitken
1998Lawen A, Baker MA, Malik S, 'Apoptosis and redox homostasis: on a possible mechanism of action of Bcl-2', PROTOPLASMA, UNIV ANTWERP, ANTWERP, BELGIUM (1998)
DOI10.1007/BF01279288Author URL
CitationsScopus - 7Web of Science - 6
Show 16 more conferences
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Grants and Funding

Summary

Number of grants26
Total funding$6,064,682

Click on a grant title below to expand the full details for that specific grant.


20152 grants / $801,477

Characterising a newly identified mechanism causing elevation of intracranial pressure after acute neurological injury$494,400

Funding body: NHMRC (National Health & Medical Research Council)

Funding bodyNHMRC (National Health & Medical Research Council)
Project TeamDoctor Neil Spratt, Doctor Mark Baker
SchemeProject Grant
RoleInvestigator
Funding Start2015
Funding Finish2015
GNoG1400231
Type Of FundingAust Competitive - Commonwealth
Category1CS
UONY

Izumo1 and its role in male infertility and male contraception$307,077

Funding body: NHMRC (National Health & Medical Research Council)

Funding bodyNHMRC (National Health & Medical Research Council)
Project TeamDoctor Mark Baker, Laureate Professor John Aitken, Dr Christopher Gordon
SchemeProject Grant
RoleLead
Funding Start2015
Funding Finish2015
GNoG1400190
Type Of FundingAust Competitive - Commonwealth
Category1CS
UONY

20132 grants / $314,000

Triple Quadrupole Mass Spectrometer with nano-infusion for targeted protein quantification$300,000

Funding body: University of Newcastle

Funding bodyUniversity of Newcastle
Project TeamDoctor Mark Baker, Laureate Professor John Aitken, Conjoint Professor Bogdan Dlugogorski, Professor Hubert Hondermarck
SchemeEquipment Grant
RoleLead
Funding Start2013
Funding Finish2013
GNoG1200137
Type Of FundingInternal
CategoryINTE
UONY

NucleoCounter SP100$14,000

Funding body: NHMRC (National Health & Medical Research Council)

Funding bodyNHMRC (National Health & Medical Research Council)
Project TeamLaureate Professor John Aitken, Associate Professor Brett Nixon, Doctor Shaun Roman, Doctor Mark Baker, Doctor Geoffry De Iuliis, Doctor Lisa Mitchell, Doctor Matthew Jobling, Doctor Zamira Gibb, Mr Nenad Naumovski
SchemeEquipment Grant
RoleInvestigator
Funding Start2013
Funding Finish2013
GNoG1201175
Type Of FundingOther Public Sector - Commonwealth
Category2OPC
UONY

20123 grants / $457,568

Understanding sperm motility for infertility and contraceptive purposes$432,568

Funding body: NHMRC (National Health & Medical Research Council)

Funding bodyNHMRC (National Health & Medical Research Council)
Project TeamDoctor Mark Baker
SchemeCareer Development Fellowships
RoleLead
Funding Start2012
Funding Finish2012
GNoG1100192
Type Of FundingAust Competitive - Commonwealth
Category1CS
UONY

BMG SPECTROstar Nano$15,000

Funding body: NHMRC (National Health & Medical Research Council)

Funding bodyNHMRC (National Health & Medical Research Council)
Project TeamLaureate Professor John Aitken, Professor Eileen McLaughlin, Ms Belinda Nixon, Doctor Shaun Roman, Doctor Mark Baker, Doctor Geoffry De Iuliis, Doctor Lisa Mitchell, Doctor Matthew Jobling
SchemeEquipment Grant
RoleInvestigator
Funding Start2012
Funding Finish2012
GNoG1100971
Type Of FundingOther Public Sector - Commonwealth
Category2OPC
UONY

PULSE Early Career Researcher$10,000

Funding body: Hunter Medical Research Institute

Funding bodyHunter Medical Research Institute
Project TeamDoctor Mark Baker
SchemePULSE Early Career Researcher of the Year Award
RoleLead
Funding Start2012
Funding Finish2012
GNoG1200578
Type Of FundingGrant - Aust Non Government
Category3AFG
UONY

20115 grants / $1,735,222

Chemical principles underpinning a spermostatic-microbiostatic agent capable of preventing pregnancy and the spread of sexually transmitted disease$735,000

Funding body: ARC (Australian Research Council)

Funding bodyARC (Australian Research Council)
Project TeamLaureate Professor John Aitken, Doctor Mark Baker, Professor Kenneth Beagley, Professor Adam McCluskey
SchemeDiscovery Projects
RoleInvestigator
Funding Start2011
Funding Finish2011
GNoG1000173
Type Of FundingAust Competitive - Commonwealth
Category1CS
UONY

Understanding the Molecular Basis of Epididymal Maturation: How Does the Epididymis Modify Spermatozoa, Allowing them to Recognise the Egg?$565,222

Funding body: NHMRC (National Health & Medical Research Council)

Funding bodyNHMRC (National Health & Medical Research Council)
Project TeamDoctor Mark Baker
SchemeProject Grant
RoleLead
Funding Start2011
Funding Finish2011
GNoG1000306
Type Of FundingAust Competitive - Commonwealth
Category1CS
UONY

An Advanced Mass Spectrometer for Applications in Phospho-Proteomics, Glycomics and Top-Down Sequencing of Proteins$250,000

Funding body: ARC (Australian Research Council)

Funding bodyARC (Australian Research Council)
Project TeamDoctor Mark Baker, Laureate Professor John Aitken, Professor Roger Smith, Conjoint Professor Keith Jones, Conjoint Professor Ray Rose, Professor Lois Salamonsen
SchemeLinkage Infrastructure Equipment & Facilities (LIEF)
RoleLead
Funding Start2011
Funding Finish2011
GNoG1000632
Type Of FundingScheme excluded from IGS
CategoryEXCL
UONY

3D Ion Trap Mass Spectrometer with ETD for Top Down Proteomics Applications$170,000

Funding body: University of Newcastle

Funding bodyUniversity of Newcastle
Project TeamDoctor Mark Baker, Laureate Professor John Aitken
SchemeEquipment Grant
RoleLead
Funding Start2011
Funding Finish2011
GNoG1000470
Type Of FundingInternal
CategoryINTE
UONY

An Advanced Mass Spectrometer for Applications in Phospho-Proteomics, Glycomics and Top-Down Sequencing of Proteins$15,000

Funding body: Prince Henry's Institute of Medical Research

Funding bodyPrince Henry's Institute of Medical Research
Project TeamDoctor Mark Baker, Laureate Professor John Aitken, Professor Roger Smith, Conjoint Professor Keith Jones, Conjoint Professor Ray Rose, Professor Lois Salamonsen
SchemeLinkage Infrastructure Equipment & Facilities (LIEF) Partner funding
RoleLead
Funding Start2011
Funding Finish2011
GNoG1100844
Type Of FundingScheme excluded from IGS
CategoryEXCL
UONY

20105 grants / $837,000

An Integrated LC-MS-NMR facility for Applications in Proteomics and Organic Chemistry$500,000

Funding body: ARC (Australian Research Council)

Funding bodyARC (Australian Research Council)
Project TeamProfessor Adam McCluskey, Laureate Professor John Aitken, Professor Paul Dastoor, Professor Phillip Robinson, Professor Eileen McLaughlin, Emeritus Professor Geoffrey Lawrance, Professor Marcel Maeder, Professor Hugh Dunstan, Doctor Shaun Roman, Professor Rob Atkin, Doctor Clovia Holdsworth, Doctor Mark Baker, Doctor Nikki Verrills, Professor Gottfried Otting, Associate Professor Brett Nixon, Doctor Xiaojing Zhou, Ms Megan Chircop, Doctor Warwick Belcher
SchemeLinkage Infrastructure Equipment & Facilities (LIEF)
RoleInvestigator
Funding Start2010
Funding Finish2010
GNoG0190402
Type Of FundingScheme excluded from IGS
CategoryEXCL
UONY

An Integrated LC-MS-NMR facility for Applications in Proteomics and Organic Chemistry$280,000

Funding body: University of Newcastle

Funding bodyUniversity of Newcastle
Project TeamProfessor Adam McCluskey, Laureate Professor John Aitken, Professor Paul Dastoor, Professor Phillip Robinson, Professor Eileen McLaughlin, Emeritus Professor Geoffrey Lawrance, Professor Marcel Maeder, Professor Hugh Dunstan, Doctor Shaun Roman, Professor Rob Atkin, Doctor Clovia Holdsworth, Doctor Mark Baker, Doctor Nikki Verrills, Professor Gottfried Otting, Associate Professor Brett Nixon, Doctor Xiaojing Zhou, Ms Megan Chircop, Doctor Warwick Belcher
SchemeEquipment Grant
RoleInvestigator
Funding Start2010
Funding Finish2010
GNoG1000873
Type Of FundingInternal
CategoryINTE
UONY

Waters, Ultra high performance liquid chromatography system (nano-Aquity) $30,000

Funding body: NHMRC (National Health & Medical Research Council)

Funding bodyNHMRC (National Health & Medical Research Council)
Project TeamDoctor Mark Baker, Laureate Professor John Aitken, Professor Adam McCluskey, Associate Professor Brett Nixon, Emeritus Professor John Rostas, Professor Eileen McLaughlin
SchemeEquipment Grant
RoleLead
Funding Start2010
Funding Finish2010
GNoG1000051
Type Of FundingOther Public Sector - Commonwealth
Category2OPC
UONY

The design of novel, affinity-based technology to specifically isolate phosphotyrosine-containing pedtides$25,000

Funding body: Hunter Children`s Research Foundation

Funding bodyHunter Children`s Research Foundation
Project TeamDoctor Mark Baker, Professor Adam McCluskey
SchemeResearch Grant
RoleLead
Funding Start2010
Funding Finish2010
GNoG0900191
Type Of FundingContract - Aust Non Government
Category3AFC
UONY

2009 FSIT Award for Research Excellence$2,000

Funding body: University of Newcastle

Funding bodyUniversity of Newcastle
Project TeamDoctor Mark Baker
SchemeAward for Research Excellence
RoleLead
Funding Start2010
Funding Finish2010
GNoG1000404
Type Of FundingInternal
CategoryINTE
UONY

20095 grants / $1,192,621

Identification and characterisation of phosphorylation events taking place during human sperm capacitation$269,250

Funding body: NHMRC (National Health & Medical Research Council)

Funding bodyNHMRC (National Health & Medical Research Council)
Project TeamDoctor Mark Baker
SchemeProject Grant
RoleLead
Funding Start2009
Funding Finish2009
GNoG0188834
Type Of FundingAust Competitive - Commonwealth
Category1CS
UONY

China-Australia therapeutic hypothermia in stroke (CATHS) research program: identification of diagnostic molecular markers and therapeutic targets involved in re-warming related brain injury$230,275

Funding body: NSW Office for Science & Medical Research

Funding bodyNSW Office for Science & Medical Research
Project TeamConjoint Professor Chris Levi, Doctor Mark Baker, Doctor Neil Spratt, Emeritus Professor John Rostas
SchemeChina-NSW Collaborative Research Program
RoleInvestigator
Funding Start2009
Funding Finish2009
GNoG0190392
Type Of FundingOther Public Sector - State
Category2OPS
UONY

Assessing the significance of the sperm thioredoxins as contraceptive targets for the male$148,096

Funding body: Contraceptive Research and Development Program

Funding bodyContraceptive Research and Development Program
Project TeamLaureate Professor John Aitken, Doctor Mark Baker
SchemeDevelopment of New or Improved Contraceptive Products
RoleInvestigator
Funding Start2009
Funding Finish2009
GNoG0189339
Type Of FundingInternational - Non Competitive
Category3IFB
UONY

20081 grants / $4,000

PULSE Education Prize$4,000

Funding body: Hunter Medical Research Institute

Funding bodyHunter Medical Research Institute
Project TeamDoctor Mark Baker
SchemePULSE Education Prize
RoleLead
Funding Start2008
Funding Finish2008
GNoG0188535
Type Of FundingContract - Aust Non Government
Category3AFC
UONY

20073 grants / $722,794

2007 Research Fellowship - PRCRS$509,053

Funding body: University of Newcastle

Funding bodyUniversity of Newcastle
Project TeamDoctor Mark Baker
SchemeResearch Fellowship
RoleLead
Funding Start2007
Funding Finish2007
GNoG0187158
Type Of FundingInternal
CategoryINTE
UONY

AMPPAII Proposal for completion of the Sptrx KO mice study$198,741

Funding body: Schering AG

Funding bodySchering AG
Project TeamLaureate Professor John Aitken, Doctor Mark Baker
SchemeResearch Networks
RoleInvestigator
Funding Start2007
Funding Finish2007
GNoG0188468
Type Of FundingContract - Aust Non Government
Category3AFC
UONY

2007 Research Fellowship Project Grant$15,000

Funding body: University of Newcastle

Funding bodyUniversity of Newcastle
Project TeamDoctor Mark Baker
SchemeFellowship Grant
RoleLead
Funding Start2007
Funding Finish2007
GNoG0188117
Type Of FundingInternal
CategoryINTE
UONY
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Research Supervision

Current Supervision

CommencedResearch Title / Program / Supervisor Type
2014Determining Factors within Cerebrospinal Fluid that Cause Intracranial Pressure to Rise Post-Stroke
Human Biology, Faculty of Health and Medicine
Co-Supervisor
2013Generation of Gene-Disrupted Mice to Further Elucidate Reproductive Mechanisms in the Male Germ Cell
Biological Sciences, Faculty of Science and Information Technology
Co-Supervisor
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Dr Mark Baker

Position

Research Fellow
School of Environmental and Life Sciences
Faculty of Science and Information Technology

Focus area

Biological Sciences

Contact Details

Emailmark.baker@newcastle.edu.au
Phone(02) 4921 7880
Fax(02) 4921 6308

Office

RoomLS4-42
BuildingLife Sciences
LocationCallaghan
University Drive
Callaghan, NSW 2308
Australia
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