Staff Profile

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Career Summary

Biography

Dr Lin Kooi Ong completed Bachelor of Biomedical Science (Honours) in 2008 and obtained a PhD in medical biochemistry from the University of Newcastle in 2012. Dr Ong is a Postdoctoral Researcher at the University of Newcastle and the HMRI Brain and Mental Health Program, investigating the role of infection in the development of Parkinson’s disease.

Dr Ong’s research focus has been on the investigation of the regulation of tyrosine hydroxylase in vivo. In this he has provided the first comprehensive analysis linking tyrosine hydroxylase phosphorylation and tyrosine hydroxylase activation in the various catecholamine neuronal groups and adrenal chromaffin cells. His recent work has focused on the influence of the immune system on the regulation of tyrosine hydroxylase. He has provided the first evidence that peripheral inflammation can induce long-term effects on the regulation of tyrosine hydroxylase in the peripheral and central catecholaminergic systems. In 2013 Dr Ong initiated a new research focus in A/Prof Phillip Dickson’s laboratory (Parkinson/Tyrosine hydroxylase laboratory) on the investigation of the role of inflammation and dopamine synthesis in the specific subset of dopaminergic neurons that are susceptible to degeneration in Parkinson’s disease. This work is currently funded by an HMRI Project Grant and a Faculty of Health and Medicine Pilot Grant.

Dr Ong has in total 12 publications since 2007, including 6 journal articles from his PhD studies. He has been first author on 4 papers and has published in respected scientific journals such as Journal of Neurochemistry, PloS One, Neuroscience and Behavioural Brain Research. Dr Ong has also presented his work at many international and national conferences; from his 20 conference publications he has been first author on 10. Since 2010, Dr Ong has 9 travel grants to attend both international and national conferences.

Qualifications

  • PhD (Medical Biochemistry), University of Newcastle, 26/07/2012
  • Bachelor of Biomedical Sciences (Hons), University of Newcastle, 11/07/2008
  • Bachelor of Biomedical Sciences, University of Newcastle, 01/08/2007

Research

Research keywords

  • Parkinson's disease
  • catecholamines
  • neurochemistry
  • protein phosphorylation
  • signal transduction
  • stress biology
  • tyrosine hydroxylase

Research expertise

My major focus has been the investigation of tyrosine hydroxylase (TH, the rate limiting enzyme of catecholamine biosynthesis) regulation. I have provided the first comprehensive analysis linking TH phosphorylation and TH activation in both cell bodies and nerve terminals in the various catecholamine neuronal groups and adrenal chromaffin cells. I have established that different catecholaminergic cells response differently in term of the temporal profile of TH phosphorylation in a range of animal models of stress. I have also established that TH activation by a range of stressors is transient and only lasts for hours.

My more recent work has focused on the influence of the immune system on TH regulation. I have established that postnatal endotoxin challenge was able to induce sustained TH activation which was maintained into adolescence and adulthood without further interventions. Recently, I have initiated a new research focus on the pathogenic mechanisms in an inflammatory animal model of Parkinson's disease.

My broad technical skills and expertise include neurochemistry, biochemical analyses, immunohistochemistry and behavioural tests on animal models of stress and Parkinson’s disease.

Languages

  • English
  • Malay

Fields of Research

CodeDescriptionPercentage
110100Medical Biochemistry And Metabolomics50
110900Neurosciences50

Memberships

Committee/Associations (relevant to research).

  • Member - The International Society for Neurochemistry
  • Member - The Asian Pacific Society for Neurochemistry
  • Member - Australasian Neuroscience Society
  • Member - International Brain Research Organization
  • Member - Australian Society for Medical Research

Awards

International Travel Grant

2014International Brain Research Organization International Travel Grant
International Brain Research Organization (Australia)
To cover travel and lodging expenses and other costs associated with the 12th Meeting of The Asia Pacific Society for Neurochemistry, Kaohsiung, Taiwan

Invitations

The role of inflammation and dopamine synthesis in Parkinson’s disease
Universiti Putra Malaysia, Malaysia (Invited Presenter)
2014
The role of inflammation and dopamine synthesis in Parkinson’s disease
The Asia Pacific Society for Neurochemistry, Taiwan (Oral Presentation)
2014
Tyrosine hydroxylase phosphorylation and activation in vivo
University of South Australia, Australia (Invited Presenter)
2012

Collaboration

Professor Deborah Hodgson, University of Newcastle

  • the role of postnatal immune challenge in neurodevelopment

Professor Rodrigo Leal, Universidade Federal de Santa Catarina

  • the role of systemic manganese exposure in parkinsonism

Professor Daniel Gelain, Federal University of Rio Grande do Sul Brazil

  • the expression of human tyrosine hydroxylase isoforms in the differentiation of neuroblastoma cells

Teaching

Teaching keywords

  • Human Anatomy and Physiology
  • Medical Biochemistry
  • Neuroscience

Teaching expertise

Musculoskeletal Anatomy

Human Bioscience

Human Molecular Science

Human Biochemistry and Cell Biology

Neuroscience

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Highlighted Publications

For publications that are currently unpublished or in-press, details are shown in italics.

YearCitationAltmetricsLink
2014Ong LK, Guan L, Damanhuri H, Goodchild AK, Bobrovskaya L, Dickson PW, Dunkley PR, 'Neurobiological consequences of acute footshock stress: Effects on tyrosine hydroxylase phosphorylation and activation in the rat brain and adrenal medulla', Journal of Neurochemistry, 128 547-560 (2014)
2012Ong LK, Sominsky L, Dickson PW, Hodgson DM, Dunkley PR, 'The sustained phase of Tyrosine hydroxylase activation in vivo', Neurochemical Research, 37 1938-1943 (2012) [C1]
2011Ong LK, Guan L, Stutz B, Dickson PW, Dunkley PR, Bobrovskaya L, 'The effects of footshock and immobilization stress on tyrosine hydroxylase phosphorylation in the rat locus coeruleus and adrenal gland', Neuroscience, 192 20-27 (2011) [C1]
2011Ong LK, Bobrovskaya L, Walker FR, Day TA, Dickson PW, Dunkley PR, 'The effect of social defeat on tyrosine hydroxylase phosphorylation in the rat brain and adrenal gland', Neurochemical Research, 36 27-33 (2011) [C1]

Publications

For publications that are currently unpublished or in-press, details are shown in italics.

Click on a category title below to expand the list of citations for that specific category.

Journal article (12 outputs)

YearCitationAltmetricsLink
2014James MH, Quinn RK, Ong LK, Levi EM, Charnley JL, Smith DW, et al., 'mTORC1 inhibition in the nucleus accumbens 'protects' against the expression of drug seeking and 'relapse' and is associated with reductions in GluA1 AMPAR and CAMKIIa levels.', Neuropsychopharmacology, 39 1694-1702 (2014) [C1]
2014Ong LK, Guan L, Damanhuri H, Goodchild AK, Bobrovskaya L, Dickson PW, Dunkley PR, 'Neurobiological consequences of acute footshock stress: Effects on tyrosine hydroxylase phosphorylation and activation in the rat brain and adrenal medulla', Journal of Neurochemistry, 128 547-560 (2014)
2013Sominsky L, Fuller EA, Bondarenko E, Ong LK, Averell L, Nalivaiko E, et al., 'Functional Programming of the Autonomic Nervous System by Early Life Immune Exposure: Implications for Anxiety', PLOS ONE, 8 (2013) [C1]
2013Bobrovskaya L, Maniam J, Ong LK, Dunkley PR, Morris MJ, 'Early Life Stress and Post-Weaning High Fat Diet Alter Tyrosine Hydroxylase Regulation and AT1 Receptor Expression in the Adrenal Gland in a Sex Dependent Manner', NEUROCHEMICAL RESEARCH, 38 826-833 (2013) [C1]
2012Ong LK, Sominsky L, Dickson PW, Hodgson DM, Dunkley PR, 'The sustained phase of Tyrosine hydroxylase activation in vivo', Neurochemical Research, 37 1938-1943 (2012) [C1]
2012Damanhuri HA, Burke PGR, Ong LK, Bobrovskaya L, Dickson PW, Dunkley PR, Goodchild AK, 'Tyrosine hydroxylase phosphorylation in catecholaminergic brain regions: A marker of activation following acute hypotension and glucoprivation', Plos One, 7 1-19 (2012) [C1]
2012Sominsky Bar L, Walker AK, Ong LK, Tynan R, Walker FR, Hodgson DM, 'Increased microglial activation in the rat brain following neonatal exposure to a bacterial mimetic', Behavioural Brain Research, 226 351-356 (2012) [C1]
2011Ong LK, Guan L, Stutz B, Dickson PW, Dunkley PR, Bobrovskaya L, 'The effects of footshock and immobilization stress on tyrosine hydroxylase phosphorylation in the rat locus coeruleus and adrenal gland', Neuroscience, 192 20-27 (2011) [C1]
2011Ong LK, Bobrovskaya L, Walker FR, Day TA, Dickson PW, Dunkley PR, 'The effect of social defeat on tyrosine hydroxylase phosphorylation in the rat brain and adrenal gland', Neurochemical Research, 36 27-33 (2011) [C1]
2011Wynne OL, Horvat JC, Kim RY, Ong LK, Smith R, Hansbro PM, et al., 'Neonatal respiratory infection and adult re-infection: Effect on glucocorticoid and mineralocorticoid receptors in the hippocampus in BALB/c mice', Brain Behavior and Immunity, 25 1214-1222 (2011) [C1]
2010Bobrovskaya L, Damanhuri HA, Ong LK, Schneider JJ, Dickson PW, Dunkley PR, Goodchild AK, 'Signal transduction pathways and tyrosine hydroxylase regulation in the adrenal medulla following glucoprivation: An in vivo analysis', Neurochemistry International, 57 162-167 (2010) [C1]
2007O'Leary MA, Schneider JJ, Krishnan BP, Lavis C, McKendry A, Ong LK, Isbister GK, 'Cross-neutralisation of Australian brown and tiger snake venoms with commercial antivenoms: Cross-reactivity or antivenom mixtures?', Toxicon, 50 206-213 (2007) [C1]
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Conference (13 outputs)

YearCitationAltmetricsLink
2014Ong LK, Briggs GD, Dunkley PR, Dickson PW, 'The role of inflammation and dopamine synthesis in Parkinson's disease', JOURNAL OF NEUROCHEMISTRY, Kaohsiung, TAIWAN (2014)
2012Sominsky Bar L, Fuller AE, Bondarenko E, Ong LK, Clark VR, Bobrovskaya L, et al., 'Neonatal programming of the autonomic nervous system by immunological challenge: Implications for anxiety', Abstracts of the 21st Annual Meeting of the International Behavioral Neuroscience Society, Kona, Hawaii (2012) [E3]
2012Ong LK, Guan L, Bobrovskaya L, Dickson PW, Dunkley PR, 'Neurobiological consequences of acute footshock stress', Journal of Neurochemistry, Kobe, Japan (2012) [E3]
2012Sominsky Bar L, Fuller EA, Bondarenko E, Ong LK, Clark VR, Bobrovskaya L, et al., 'Neonatal immune challenge induces anxiety in adulthood and is associated with functional alterations to the autonomic nervous system', Brain, Behavior, and Immunity, San Diego, CA (2012) [E3]
2011Sominsky Bar L, Walker AK, Ong LK, Tynan R, Walker FR, Hodgson DM, 'Epigenetic inheritance of anxiety-like behaviour in rats - Role of early life exposure to a bacterial mimetic', Journal of Developmental Origins of Health and Disease, Portland, Oregon (2011) [E3]
2011Wynnea O, Horvat JC, Kim RY, Ong LK, Smith R, Hansbro PM, et al., 'Sex differences in the effect of neonatal infection and adult re-infection on hippocampal corticosterone receptors and stress response outcomes', Brain, Behavior, and Immunity, Chicago, Illinois (2011) [E3]
2011Sominsky Bar L, Walker AK, Ong LK, Walker FR, Hodgson DM, 'Postnatal exposure to a bacterial mimetic increases microglial activation and histone H3 acetylation in rats', Brain, Behavior, and Immunity, Chicago, Illinois (2011) [E3]
2010Ong LK, Guan L, Stutz B, Dickson PW, Dunkley PR, Bobrovskaya L, 'Tyrosine hydroxylase phosphorylation in response to footshock and restraint stress', Journal of Neurochemistry, Phuket, Thailand (2010) [E3]
2009Ong LK, Maniam J, Dunkley PR, Bobrovskaya L, Morris MJ, 'The effect of voluntary exercise and high fat diet on tyrosine hydroxylase phosphorylation in male Sprague-Dawley rats exposed to early life stress', Autonomic Neuroscience: Basic and Clinical, Sydney, NSW (2009) [E3]
2009Bobrovskaya L, Damanhuri H, Ong LK, Dickson PW, Dunkley PR, Goodchild AK, 'The effect of glucoprivation on tyrosine hydroxylase phosphorylation in adrenals of Sprague-Dawley rats', Autonomic Neuroscience: Basic and Clinical, Sydney, NSW (2009) [E3]
2009Ong LK, Bobrovskaya L, Walker FR, Day TA, Dickson PW, Dunkley PR, 'The effect of social conflict on tyrosine hydroxylase phosphorylation in catecholamine-producing cells from sprague-dawley rats', Journal of Neurochemistry, Busan, Korea (2009) [E3]
2009Damanhuri H, Ong LK, Bobrovskaya L, Dunkley PR, Goodchild AK, 'Activation of tyrosine hydroxylase in the A8, A9 and A10 cell groups following hypotension and glucoprivation in Sprague Dawley rat', Autonomic Neuroscience: Basic and Clinical, Sydney, NSW (2009) [E3]
2009Bobrovskaya L, Ong LK, Walker RA, Day TA, Dickson PW, Dunkley PR, 'The effect of social stress on tyrosine hydroxylase phosphorylation', ANS 2009 Abstracts: Posters, Canberra, ACT (2009) [E3]
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Grants and Funding

Summary

Number of grants1
Total funding$24,596

- Indicates that the researcher may be seeking students for this project.

Click on a grant title below to expand the full details for that specific grant.

2013 (1 grants)

Ultra-Low Temperature Cryogenic Freezer$24,596
Funding Body: NHMRC (National Health & Medical Research Council)

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Research Supervision

Number of current supervisions0

No Supervisions.

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Dr Lin Kooi Ong

Work Phone(02) 49215736
Email
PositionResearch Associate
Tyrosine Hydroxylase Laboratory
School of Biomedical Sciences and Pharmacy
Faculty of Health and Medicine
The University of Newcastle, Australia
Office
LS339,
Life Sciences Building ,
Callaghan
University Drive
Callaghan NSW 2308
Australia
URL:www.newcastle.edu.au/profile/linkooi-ong