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Emeritus Professor John Rostas

Emeritus Professor

Faculty of Health and Medicine

Career Summary

Biography

I have a distinguished research record in the study of molecular mechanisms of synaptic plasticity in many experimental systems, particularly in studies of brain development and maturation. I have always maintained a multidisciplinary approach in my research and active collaborations with colleagues in different disciplines both in Australia and overseas.

I have an international reputation in two main areas: 1. I am an internationally recognised authority on the role and regulation of the important regulatory enzyme CaMKII, as evidenced by my invitation to be a keynote speaker on this topic in an international symposium at the joint meeting of the International and European Societies for Neurochemistry in Innsbruck, Austria (August, 2005). 2. I am internationally recognised for developing the maturing chicken forebrain as an animal model for the slow maturation of brain observed in humans and for using biochemical, electrophysiological, anatomical and behavioural techniques in chickens to investigate the fundamental biological changes that occur during maturation of the brain.

Evidence for my recognition is an invitation to be a major speaker, and to chair a session, at the forthcoming Brain Mechanisms, Cognition and Behaviour in Birds Satellite Conference to the IBRO (International Brain Research Organisation) World Congress of Neuroscience (July 2007). I am also an invited Editor for the special issue of Brain Research Bulletin that will publish papers from this conference.

When comparing my scientific research track record against those of others, it must be noted that I have always performed my research while holding full academic roles that required major educational and administrative input. The extent of my activities in education and curriculum development is reflected by two peer reviewed publications in the area and invitations to speak at international conferences on problem based learning.

In the last 10 years I have continuously held senior academic administrative responsibilities (Assistant and Deputy Dean of the Faculty of Medicine and Health Sciences, Executive Director of the Hunter Medical Research Institute, Deputy Head of Faculty of Health) and been appointed to State and Commonwealth government advisory panels and chairmanships, including 9 years on the NHMRC Project Grants Committee.

Despite these responsibilities I have continued to publish 3-5 papers per year in high quality, international peer reviewed journals (~50% of papers appeared in journals with an impact factor above 4.8 and 70% above 3). In all, I have published more than 95 papers in high ranking international journals. The fact that much of my research has been performed in chickens accounts for the lower citation rate compared with similar work performed in mammals. My research papers have had a consistent citation rate of 70-90 per year for the last decade. I have received multiple invitations to speak at international and national conferences including the inaugural Lawrie Austin Plenary Lecture at the 2002 meeting of the Australian Neuroscience Society.

Research Expertise
I have a distinguished research record in the study of molecular mechanisms of synaptic plasticity in many experimental systems, particularly in studies of brain development and maturation. I have always maintained a multidisciplinary approach to research and active collaborations with colleagues in different disciplines both in Australia and overseas.

My research expertise is focused in two areas: 1. I am an internationally recognised authority on the role and regulation of the important regulatory enzyme CaMKII. In this field work from my laboratory was the first to show : " That developmental changes in subcellular distribution of CaMKII occur independently of the developmental change in expression level of the enzyme and that the subcellular translocation is indirectly regulated by thyroid hormone " The existence of giant mossy fibre nerve terminals in the chicken hippocampus and of LTP and LTD at these synapses, and to show that CaMKII activation is required for presynaptic LTD at these synapses. " That CaMKII regulation in vivo cannot be explained by currently known mechanisms and that there is a new class of regulatory mechanism operating in vivo. This new class of control mechanisms apparently occur only a specific functionally important locations in neurons (e.g. at the PSD in association with certain receptors) and require interaction with specific local proteins. If this is true, these proteins will provide highly selective drug targets through which the activity of CaMKII could be altered at specific locations in the neuron without altering CaMKII activity elsewhere in neurons or in other cells. This could lead to neuro/psycho-active drugs with fewer side-effects. These activation of these new control mechanisms could also be used as biomarkers of disease or neuropathology. 2. I am internationally recognised for developing the maturing chicken forebrain as an animal model for the slow maturation of brain observed in humans. Work from my laboratory has demonstrated the protracted maturation of neuronal function in chicken brain using biochemical, electrophysiological and anatomical techniques and identified some fundamental changes in biological mechanisms during maturation. The chicken model for brain maturation may provide opportunities to identify the major biological factors controlling the maturation process and to test a variety of drugs or other interventions on the normal progress of this maturation. This is of relevance to a variety of neurodevelopmental disorders.

Teaching Expertise
Supervised 14 postgraduate research students and 7 postdoctoral research fellows. Developed and taught integrated, problem based curricula for Bachelor of Medicine and Bachelor of Biomedical Science. Developed training programs for research planning and research grant preparation for postgraduate research students and university and hospital staff. Invited to speak on curriculum design and evaluation at major international centres in Europe and North America. Peer reviewed publications on problem based curriculum design: Rostas, J.A.P. and Olson,L.G. (1997). Teaching basic sciences in an integrated curriculum: making it work. In: Imperatives in Medical Education: The Newcastle Approach. Eds. R. Henry, K. Byrne and C. Engel. University of Newcastle, Australia, pp 83-94. Rostas, J.A.P. and Rolfe, I. (1997). Training working problem tutors for medical students. In: Imperatives in Medical Education: The Newcastle Approach, Eds. R. Henry, K. Byrne and C. Engel. University of Newcastle, Australia, pp 189-199. External Examiner for University of Papua New Guinea Medical School (1996), Royal Australian College of Obstetricians and Gynaecologists (1989-1994), and PhD theses for Sydney, Melbourne, Monash, New England and RMIT Universities.

Administrative Expertise
SELECTED SENIOR ADMINISTRATIVE APPOINTMENTS (since 1992) 2006 - Deputy Head of Faculty (Research), and Professor of Biomedical Sciences, Faculty of Health, University of Newcastle - responsible for coordinating research and research training across the Faculty at all campuses 1999 - 2006 Founding Executive Director, Hunter Medical Research Institute and Professor of Biomedical Sciences, Faculty of Health, The University of Newcastle - established the scientific organisation and governance foundations of HMRI, its recognition by the NSW Government as the third largest medical research institute in the state, and its infrastructure funding by NSW government on an equal footing with Sydney institutes. 1997 - 2001 Assistant Dean (Research), Faculty of Medicine & Health Sciences, University of Newcastle Chairmanship or Sole Responsibility 2001 - 2006 Chair, Complaints Subcommittee of NH&MRC Projects Grants Committee 2003 - 2006 Deputy Chair, NH&MRC Project Grants Committee 2003 - 2004 Chair, BioFirst High Bandwidth Network Implementation Committee, NSW Government 2002 - 2003 Chair, BioFirst High Bandwidth Network Committee, NSW Government 1997 - 2001 Assistant Dean (Research) and Chair of the Faculty Research Management Committee, Faculty of Medicine and Health Sciences, University of Newcastle 1996 - 1997 Chair, Local Organising Committee for 17th Annual Conference of the Australian Neuroscience Society 1992 - 1995 Chair, International Society for Neurochemistry Committee for Aid for Neurochemistry 1984 - 1995 Editor and member of Council Executive for Australian Neuroscience Society 2006 - Deputy Chair, Research Training Committee of The University of Newcastle Committee Membership 1997 - 2006 Project Grants Committee, National Health and Medical Research Council of Australia 2003 - 2006 Ministerial Advisory Committee on Medical and Health Research, NSW Government 2001 - 2006 Ministerial Advisory Committee on Biotechnology, NSW Government 2001 - 2006 BioFirst Awards Committee, NSW Government 1999 - 2004 Research and Development Advisory Committee, NSW Department of Health 1998 - 2006 Council of Asian Pacific Society for Neurochemistry 2003 - 2005 Cancer Research Advisory Board, Cancer Institute of NSW 1991 - 1995 Council of the International Society for Neurochemistry 1999 - 2004 Reference Group for Medical Research, Australian Business


Qualifications

  • PhD, Monash University
  • Bachelor of Science (Honours), Monash University

Keywords

  • biochemistry
  • cell biology
  • neuroscience

Fields of Research

CodeDescriptionPercentage
110199Medical Biochemistry and Metabolomics not elsewhere classified40
170299Cognitive Sciences not elsewhere classified20
110999Neurosciences not elsewhere classified40

Professional Experience

UON Appointment

DatesTitleOrganisation / Department
24/11/2014 - 24/11/2014Casual AcademicUniversity of Newcastle
School of Medicine and Public Health
Australia
1/07/2013 - 31/12/2013ProfessorUniversity of Newcastle
School of Biomedical Sciences and Pharmacy
Australia

Academic appointment

DatesTitleOrganisation / Department
1/01/2003 - 1/08/2006Ministerial Advisory Committee on Medical and Health ResearchNSW Government
Australia
1/09/1999 - 1/08/2006Executive Director of Hunter Medical Research InstituteUniversity of Newcastle
Australia
1/09/1991 - 1/09/1995Council of the International Society for Neurochemistry and Chair of its Committee for Aid for NeurochemistryInternational Society for Neurochemistry
United Kingdom

Membership

DatesTitleOrganisation / Department
1/01/2006 - 31/12/2006Chair of Grant Review Panel - Molecular and Cellular NeuroscienceNHMRC Committee
Australia
1/01/1995 - member - Neurochemical Research (scientific journal)Neurochemical Research
Australia

Invitations

Participant

YearTitle / Rationale
2005Keynote speaker and Symposium Co-Chair
Organisation: International Society for Neurochemistry Description: Our proposal for a Symposium at the biennial conference (held jointly with the European Society for Neurochemistry) was chosen for inclusion in the final program from a field of more than 200 proposals. The four speakers came from UK, Netherlands, USA and Australia (me).
2002Lawrie Austin Plenary Lecture
Organisation: Australian Neuroscience Society Description: It is an honour to be invited by the ANS Council to present this plenary lecture named after one of the founders of the Scociety. I was the first recipient of this honour which was launched on the occasion of the joint meeting of ANS with the International Society for Developmental Neuroscience in Sydney.
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Publications

For publications that are currently unpublished or in-press, details are shown in italics.


Chapter (2 outputs)

YearCitationAltmetricsLink
2012Skelding KA, Rostas JA, 'The role of molecular regulation and targeting in regulating calcium/calmodulin stimulated protein kinases', Calcium Signaling, Springer New York LLC, New York, NY 703-730 (2012) [B1]
CitationsScopus - 4Web of Science - 4
Co-authorsKathryn Skelding
2010Atkinson RJ, Rosso OA, Figliola A, Serrano E, Moscato PA, Hunter M, Rostas JA, 'Use of the domestic chicken to investigate mechanisms of brain maturation', Translational Neuroscience and Its Advancement of Animal Research Ethics, Nova Science Publishers, Hauppauge 29-53 (2010) [B1]
Co-authorsPablo Moscato, Mick Hunter

Journal article (138 outputs)

YearCitationAltmetricsLink
2014Skelding KA, Arellano JM, Powis DA, Rostas JA, 'Excitotoxic stimulation of brain microslices as an in vitro model of stroke.', J Vis Exp, e51291 (2014) [C1]
DOI10.3791/51291Author URL
Co-authorsKathryn Skelding
2014Hoffman A, Carpenter H, Kahl R, Watt LF, Dickson PW, Rostas JAP, et al., 'Dephosphorylation of CaMKII at T253 controls the metaphase-anaphase transition', Cellular Signalling, 26 748-756 (2014) [C1]

Calcium/calmodulin-stimulated protein kinase II (CaMKII) is a multi-functional serine/threonine protein kinase that controls a range of cellular functions, including proliferation. The biological properties of CaMKII are regulated by multi-site phosphorylation and targeting via interactions with specific proteins. To investigate the role specific CaMKII phosphorylation sites play in controlling cell proliferation and cell cycle progression, we examined phosphorylation of CaMKII at two sites (T253 and T286) at various stages of the cell cycle, and also examined the effects of overexpression of wild-type (WT), T286D phosphomimic, T253D phosphomimic and T253V phosphonull forms of CaMKIIa in MDA-MB-231 breast cancer and SHSY5Y neuroblastoma cells on cellular proliferation and cell cycle progression. We demonstrate herein that whilst there is no change in total CaMKII expression or T286 phosphorylation throughout the cell cycle, a marked dephosphorylation of CaMKII at T253 occurs during the G2 and/or M phases. Additionally, we show by molecular inhibition, as well as pharmacological activation, that protein phosphatase 2A (PP2A) is the phosphatase responsible for this dephosphorylation. Furthermore, we show that inducible overexpression of WT, T286D and T253V forms of CaMKIIa in MDA-MB-231 and SHSY5Y cells increases cellular proliferation, with no alteration in cell cycle profiles. By contrast, overexpression of a T253D phosphomimic form of CaMKIIa significantly decreases proliferation, and cells accumulate in mitosis, specifically in metaphase. Taken together, these results strongly suggest that the dephosphorylation of CaMKII at T253 is involved in controlling the cell cycle, specifically the metaphase-anaphase transition. © 2014 Elsevier Inc.

DOI10.1016/j.cellsig.2013.12.015
CitationsScopus - 1Web of Science - 1
Co-authorsNikki Verrills, Phil Dickson, Kathryn Skelding
2014Abdul Majeed ABB, Pearsall E, Carpenter H, Brzozowski J, Dickson PW, Rostas JAP, Skelding KA, 'CaMKII Kinase Activity, Targeting and Control of Cellular Functions: Effect of Single and Double Phosphorylation of CaMKIIa', Calcium Signaling, 1 36-51 (2014) [C1]
Co-authorsPhil Dickson, Kathryn Skelding
2012Skelding KA, Dickson PW, Verrills NM, Rostas JA, 'Progression through mitosis can be controlled by dephosphorylation of CaMKII at T253', JOURNAL OF NEUROCHEMISTRY, 123 31-31 (2012) [E3]
Author URL
Co-authorsNikki Verrills, Kathryn Skelding, Phil Dickson
2012Majeed ABA, Rostas JAP, Carpenter H, Dickson PW, Skelding KA, 'Multi-site phosphorylation of CaMKII regulates CaMKII function co-operatively', JOURNAL OF NEUROCHEMISTRY, 123 116-116 (2012) [E3]
Author URL
Co-authorsKathryn Skelding, Phil Dickson
2012Skelding KA, Spratt NJ, Fluechter L, Dickson PW, Rostas JA, 'alpha CaMKII is differentially regulated in brain regions that exhibit differing sensitivities to ischemia and excitotoxicity', Journal of Cerebral Blood Flow and Metabolism, 32 2181-2192 (2012) [C1]
CitationsScopus - 5Web of Science - 6
Co-authorsKathryn Skelding, Neil Spratt, Phil Dickson
2011Skelding KA, Rostas JA, Verrills NM, 'Controlling the cell cycle: The role of calcium/calmodulin-stimulated protein kinases I and II', Cell Cycle, 10 631-639 (2011) [C1]
DOI10.4161/cc.10.4.14798
CitationsScopus - 26Web of Science - 28
Co-authorsKathryn Skelding, Nikki Verrills
2010Atkinson RJ, Rostas JA, Hunter M, 'Changes in mid-to-late latency auditory evoked responses in the chicken during neural maturation', Developmental Psychobiology, 52 24-34 (2010) [C1]
DOI10.1002/dev.20408
Co-authorsMick Hunter
2010Skelding KA, Suzuki T, Gordon SL, Xue J, Verrills NM, Dickson PW, Rostas JA, 'Regulation of CaMKII by phospho-Thr253 or phospho-Thr286 sensitive targeting alters cellular function', Cellular Signalling, 22 759-769 (2010) [C1]
DOI10.1016/j.cellsig.2009.12.011
CitationsScopus - 9Web of Science - 9
Co-authorsNikki Verrills, Kathryn Skelding, Phil Dickson
2009Rosso OA, Mendes ADS, Rostas JA, Hunter M, Moscato PA, 'Distinguishing childhood absence epilepsy patients from controls by the analysis of their background brain electrical activity', Journal of Neuroscience Methods, 177 461-468 (2009) [C1]
DOI10.1016/j.jneumeth.2008.10.017
CitationsScopus - 8Web of Science - 9
Co-authorsMick Hunter, Pablo Moscato
2009Rosso OA, Mendes ADS, Berretta RE, Rostas JA, Hunter M, Moscato PA, 'Distinguishing childhood absence epilepsy patients from controls by the analysis of their background brain electrical activity (II): A combinatorial optimization approach for electrode selection', Journal of Neuroscience Methods, 181 257-267 (2009) [C1]
DOI10.1016/j.jneumeth.2009.04.028
CitationsScopus - 9Web of Science - 9
Co-authorsMick Hunter, Pablo Moscato, Regina Berretta
2009Skelding KA, Rostas JA, 'Regulation of CaMKII in vivo: The importance of targeting and the intracellular microenvironment', Neurochemical Research, 34 1792-1804 (2009) [C1]
DOI10.1007/s11064-009-9985-9
CitationsScopus - 22Web of Science - 23
Co-authorsKathryn Skelding
2008Gurd JW, Rawof S, Zhen Huo J, Dykstra C, Bissoon N, Teves L, et al., 'Ischemia and status epilepitcus result in enhanced phosphorylation of calcium and calmodulin-stimulated protein kinase II on threonine 253', Brain Research, 1218 158-165 (2008) [C1]
DOI10.1016/j.brainres.2008.04.040
CitationsScopus - 6Web of Science - 7
2008Atkinson RJ, Migues PV, Hunter M, Rostas JA, 'Molecular changes in the intermediate medial mesopallium after a one trial avoidance learning in immature and mature chickens', Journal of Neurochemistry, 104 891-902 (2008) [C1]
DOI10.1111/j.1471-4159.2007.05060.x
CitationsScopus - 3Web of Science - 3
Co-authorsMick Hunter
2008Atkinson RJ, Migues PV, Cammarota M, Kavanagh JM, Hunter M, Rostas JA, 'Biochemical, behavioural and electrophysiological investigations of brain maturation in chickens', Brain Research Bulletin, 76 217-223 (2008) [C1]
DOI10.1016/j.brainresbull.2008.02.036
CitationsScopus - 5Web of Science - 4
Co-authorsMick Hunter
2008Gibbs ME, Gibbs C, Csillag A, Matsushima T, Rogers L, Rostas JA, 'Brain mechanisms, cognition and behaviour in birds', Brain Research Bulletin, 76 167-169 (2008) [C3]
DOI10.1016/j.brainresbull.2008.03.012
CitationsScopus - 4Web of Science - 3
2007Migues PV, Cammarota MP, Kavanagh J, Atkinson RJ, Powis DA, Rostas JA, 'Maturational changes in the subunit composition of AMPA receptors and the functional consequences of their activation in chicken forebrain', Developmental Neuroscience, 29 232-240 (2007) [C1]
DOI10.1159/000096408
CitationsScopus - 14Web of Science - 14
2006Sim AT, Herd LM, Proctor DT, Baldwin ML, Meunier FA, Rostas JA, 'High throughput analysis of endogenous glutamate release using a fluorescence plate reader', Journal of Neuroscience Methods, 153 43-47 (2006) [C1]
DOI10.1016/j.jneumeth.2005.10.004
CitationsScopus - 2Web of Science - 2
Co-authorsAlistair Sim, Lynn Herd
2006Baldwin ML, Cammarota MP, Sim AT, Rostas JA, 'Src family tyrosine kinases differentially modulate exocytosis from rat brain nerve terminals', Neurochemistry International, 49 80-86 (2006) [C1]
DOI10.1016/j.neuint.2006.01.002
CitationsScopus - 10Web of Science - 9
Co-authorsAlistair Sim
2006Migues PV, Lehmann IT, Fluechter L, Cammarota MP, Gurd JW, Sim AT, et al., 'Phosphorylation of CaMKII at Thr253 occurs in vivo and enhances binding to isolated postsynaptic densities', Journal of Neurochemistry, 98 289-299 (2006) [C1]
DOI10.1111/j.1471-4159.2006.03876.x
CitationsScopus - 24Web of Science - 22
Co-authorsPhil Dickson, Alistair Sim
2005Rosso OA, Hyslop WA, Gerlach RH, Smith RL, Rostas JA, Hunter M, 'Quantitative EEG analysis of the maturational changes associated with childhood absence epilepsy', Physica A -Statistical Mechanics and Its Applications, 356 184-189 (2005) [C1]
DOI10.1016/j.physa.2005.05.034
CitationsScopus - 12Web of Science - 12
Co-authorsMick Hunter
2005Hunter M, Smith RLL, Hyslop W, Rosso OA, Gerlach R, Rostas JAP, et al., 'The Australian EEG database', CLINICAL EEG AND NEUROSCIENCE, 36 76-81 (2005) [C2]
Author URL
CitationsScopus - 20Web of Science - 14
Co-authorsMick Hunter, Frans Henskens
2004Xue J, Tsang C, Gai W-P, Malladi C, Trimble W, Rostas JA, Robinson P, 'Septin 3 (G-septin) is a developmentally regulated phosphoprotein enriched in presynaptic nerve terminals', Journal of Neurochemistry, 91(3) 579-590 (2004) [C1]
DOI10.1111/j.1471-4159.2004.02755.x
CitationsScopus - 49Web of Science - 45
2004Xue J, Milburn P, Hanna B, Graham M, Rostas JA, Robinson P, 'Phosphorylation of septin 3 on Ser-91 by cGMP-dependent protein kinase-I in nerve terminals', Biochem J, 381 753-760 (2004) [C1]
DOI10.1042/BJ20040455
CitationsScopus - 23Web of Science - 16
2004Lengyel I, Voss K, Cammarota MP, Bradshaw K, Brent VA, Murphy KPSJ, et al., 'Autonomous activity of CaMKII is only transiently increased following the induction of long-term potentiation in the rat hippocampus', European Journal of Neuroscience, 20 3063-3072 (2004) [C1]
DOI10.1111/j.1460-9568.2004.03748.x
CitationsScopus - 57Web of Science - 58
2003Hunter M, Rostas JA, Hyslop WA, 'The John Hunter Hospital EEG database project', Australian Journal of Psychology, 55 104 (2003) [C3]
Co-authorsMick Hunter
2003Baldwin ML, Rostas JA, Sim AT, 'Two modes of excytosis from synaptosomes are differentially regulated by protein phosphatase types 2A and 2B', Journal of Neurochemistry, 85 1190-1199 (2003) [C1]
DOI10.1046/j.1471-4159.2003.01779.x
CitationsScopus - 26Web of Science - 25
Co-authorsAlistair Sim
2003Cammarota MP, Bevilaqua L, Rostas JA, Dunkley PR, 'Histamine activates tyrosine hydroxylase in bovine adrenal chromaffin cells through a pathway that involves ERK1/2 but not p38 or JNK', Journal of Neurochemistry, 84 453-458 (2003) [C1]
DOI10.1046/j.1471-4159.2003.01517.x
CitationsScopus - 25Web of Science - 25
Co-authorsPeter Dunkley
2003Sim AT, Baldwin ML, Rostas JA, Holst J, Ludowyke RI, 'The role of serine/threonine protein phosphatases in exocytosis', Biochemical Journal, 373 641-659 (2003) [C1]
DOI10.1042/BJ20030484
CitationsScopus - 37Web of Science - 36
Co-authorsAlistair Sim
2003Atkinson RJ, Bevilaqua L, Rostas JA, Hunter M, 'Discriminative taste aversion learning: A learning task for older chickens', Neurobiology of Learning and Memory, 79 25-31 (2003)
DOI10.1016/S1074-7427(02)00011-4
CitationsScopus - 5Web of Science - 5
Co-authorsMick Hunter
2001Lengyel I, Voss K, Brent V, Cammarota M, Murphy K, Rostas JAP, Bliss TVP, 'Activation and autophosphorylation of CaMPK-II under conditions that do or do not induce LTP', JOURNAL OF NEUROCHEMISTRY, 78 13-13 (2001)
Author URL
2001Lengyel I, Cammarota MP, Brent VA, Rostas JA, 'Autonomous activity and autophosphorylation of CAMPK-II in rat hippocampal slices: effects of tissue preparation', Journal of Neurochemistry, 76 149-154 (2001) [C1]
CitationsScopus - 8Web of Science - 8
2001Cammarota MP, Bevilaqua L, Dunkley PR, Rostas JA, 'Angiotensin II Promotes the Phosphorylation of Cyclic AMP-Responsive Element Binding Protein (CREB) at Ser133 Through an ERK1/2-Dependent Mechanism', Journal of Neurochemistry, 79 1122-1128 (2001) [C1]
CitationsScopus - 32Web of Science - 31
Co-authorsPeter Dunkley
2001Lengyel I, Nairn AC, McCluskey A, Toth G, Penke B, Rostas JAP, 'Auto-inhibition of Ca2+/calmodulin-dependent protein kinase II by its ATP-binding domain', Journal of Neurochemistry, 76 1066-1072 (2001) [C1]
CitationsScopus - 10Web of Science - 8
Co-authorsAdam Mccluskey
2001Cammarota MP, Bevilaqua L, Dunkley PR, Rostas JA, 'Angiotensin II Promotes a Rapid and Reversible Increase in SRC-Tyrosine Kinase Activity in Bovine Adrenal Chromaffin Cells: Effect on MAPK Pathways', Proceedings of the Australian Neuroscience Society, 12 197 (2001) [C3]
Co-authorsPeter Dunkley
2001Cammarota MP, Bevilaqua L, Dunkley PR, Rostas JA, 'Angiotensin II Promotes a Src and ERK 1/2 Dependent Increase in SER133-CREB Phosphorylation', Journal of Neurochemistry, 78 Supl. 1 143 (2001) [C3]
Co-authorsPeter Dunkley
2000Hunter M, Battilana M, Bragg T, Rostas J, 'EEG as a measure of developmental changes in the chicken brain', Developmental Psychobiology, 36 23-28 (2000) [C1]
CitationsScopus - 13Web of Science - 12
Co-authorsMick Hunter
2000Lengyel I, Fieuw-Makaroff S, Hall AL, Sim AT, Rostas JA, Dunkley PR, 'Modulation of the Phosphorylation and Activity of Calcium/Calmodulin-Dependent Protein Kinase II by Zinc', Journal of Neurochemistry, 75 No. 2 594-605 (2000) [C1]
CitationsScopus - 51Web of Science - 51
Co-authorsPeter Dunkley, Alistair Sim
2000Margrie TW, Rostas JA, Sah P, 'Inhibition of transmitter release and long-term depression in the avian hippocampus', Neuroscience Letters, 284 17-20 (2000) [C1]
CitationsScopus - 4Web of Science - 3
2000Xue J, Wang X, Malladi CS, Kinoshita M, Milburn PJ, Lengyel I, et al., 'Phosphorylatin of a new brain-specific septin, G-Septin, by cyclic-GMP dependent protein kinase', Biological Chemistry, 275 10047-10056 (2000) [C1]
CitationsScopus - 46Web of Science - 41
1999Lengyel I, Hall AL, Fieuw-Makaroff S, Sim ATR, Rostas JAP, Dunkley PR, 'Characterization of the zinc-dependent autophosphorylation sites on calcium/calmodulin-dependent protein kinase II', JOURNAL OF NEUROCHEMISTRY, 73 S33-S33 (1999)
Author URL
Co-authorsPeter Dunkley
1999Lengyel I, Brent V, Rostas JAP, 'Autonomous CaMPK-II activity in rat hippocapal slices: Effects of tissue handling', JOURNAL OF NEUROCHEMISTRY, 73 S36-S36 (1999)
Author URL
1999Rostas JAP, Sievert AD, McCoy K, Lengyel I, 'Mossy fibre-like synapses in chicken hippocampus', JOURNAL OF NEUROCHEMISTRY, 73 S40-S40 (1999)
Author URL
1999Lengyel I, Olson LG, Nichol K, Brain K, Wang X, Robinson PJ, et al., 'Phosphorylation of proteins in chick ciliary ganglion under conditions that induce long-lasting changes in synaptic transmission: phosphoprotein targets for nitric oxide action', Neuroscience, 90 No. 2 607-619 (1999) [C1]
CitationsScopus - 5Web of Science - 3
1998Rostas JAP, 'Nitric oxide responsive protein phosphorylation in neuronal tissue', JOURNAL OF NEUROCHEMISTRY, 70 S4-S4 (1998)
Author URL
1998Capon RJ, Rooney F, Murray LM, Collins E, Sim AT, Rostas JA, et al., 'Dragmacidins: New protein phosphatase inhibitors from a Southern Australian deep-water marine sponge Spongosorites sp', Journal of Natural Products, 61 660-662 (1998) [C1]
CitationsScopus - 111Web of Science - 102
Co-authorsAlistair Sim
1998Wang X, Rostas JA, 'Protein phosphorylation in fast and slow chicken skeletal muscles: Effect of denervation', Muscle and Nerve, 21 504-513 (1998) [C1]
CitationsScopus - 2Web of Science - 2
1998Margrie TW, Rostas JA, Sah P, 'Presynaptic long-term depression at a central glutamatergic synapse: a role for CaMKII', Nature Neuroscience, 1(5) 378-383 (1998) [C1]
CitationsScopus - 13Web of Science - 26
1998Lengyel I, Nichol KA, Bennett MR, Heath JW, Little GJ, Rostas JA, 'Alpha and beta subunits of CaM-kinase II are localized in different neurons in chick ciliary ganglion', NeuroReport, 9(12) 2753-2755 (1998) [C1]
CitationsScopus - 2Web of Science - 2
1998Sim AT, Collins E, Mudge LM, Rostas JA, 'Developmental regulation of protein phosphatase types 1 and 2A in post-hatch chicken brain', Neurochemical Research, 23(4) 487-491 (1998) [C1]
CitationsWeb of Science - 11
Co-authorsAlistair Sim
1998Margrie TW, Rostas JA, Sah P, 'Long-term potentiation of synaptic transmission in the avian hippocampus', The Journal of Neuroscience, 18(4) 1207-1216 (1998) [C1]
CitationsScopus - 41Web of Science - 39
1997Sansom AJ, Brent VA, Jarvie PE, Darlington CL, Smith PF, Laverty R, Rostas JAP, 'In vitro phosphorylation of medial vestibular nucleus and prepositus hypoglossi proteins during behavioural recovery from unilateral vestibular deafferentation in the guinea pig', BRAIN RESEARCH, 778 166-177 (1997)
DOI10.1016/S0006-8993(97)01059-7Author URL
CitationsScopus - 18Web of Science - 17
1997Margrie TW, Rostas JAP, 'Subcellular translocation of Ca2+(calmodulin-dependent protein kinase II: Fact or artifact?', JOURNAL OF NEUROCHEMISTRY, 69 435-436 (1997)
Author URL
CitationsWeb of Science - 2
1997Margrie TW, Rostas JAP, Sah P, 'NMDA receptor independent long-term potentiation in the avian hippocampus', JOURNAL OF NEUROCHEMISTRY, 69 S66-S66 (1997)
Author URL
1997Lengyel I, Olesen L, Wang X, Nichol KA, Bennett MR, Rostas PJ, Rostas JAP, 'Nitric oxide responsive phosphoproteins (NORPs) in chicken ciliary ganglion', JOURNAL OF NEUROCHEMISTRY, 69 S187-S187 (1997)
Author URL
1997Sansom AJ, Brent VA, Jarvie PE, Darlington CL, Smith CL, Laverty R, Rostas JAP, 'In vitro phosphorylation of medial vestibular nucleus and prepositus hypoglossi proteins during behavioural recovery from unilateral vestibular deafferentation in the guinea pig', JOURNAL OF NEUROCHEMISTRY, 69 S208-S208 (1997)
Author URL
1997Margrie TW, Rostas JAP, Suzuki T, 'Subcellular translocation of Ca2+/calmodulin-dependent protein kinase II: Fact or artifact? [1] (multiple letters)', Journal of Neurochemistry, 69 435-437 (1997)
CitationsScopus - 4
1996Wang X, Rostas JAP, 'Effect of hypothyroidism on the subcellular distribution of Ca2+ calmodulin-stimulated protein kinase II in chicken brain during posthatch development', JOURNAL OF NEUROCHEMISTRY, 66 1625-1632 (1996)
Author URL
CitationsScopus - 7Web of Science - 8
1996Rostas JAP, Brent VA, Voss K, Errington ML, Bliss TVP, Gurd JW, 'Enhanced tyrosine phosphorylation of the 2B subunit of the N-methyl-D-aspartate receptor in long-term potentiation', PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 93 10452-10456 (1996)
DOI10.1073/pnas.93.19.10452Author URL
CitationsScopus - 186Web of Science - 190
1996Lengyel I, Nichol KA, Sim ATR, Bennett MR, Dunkley PR, Rostas JAP, 'Characterization of protein kinase and phosphatase systems in chick ciliary ganglion', NEUROSCIENCE, 70 577-588 (1996)
DOI10.1016/0306-4522(95)00356-8Author URL
CitationsScopus - 8Web of Science - 9
Co-authorsPeter Dunkley
1995KAVANAGH JM, BUNN SJ, BOYD TL, ROSTAS JAP, 'DEVELOPMENTAL-CHANGES IN GLUTAMATE-RECEPTOR STIMULATED INOSITOL PHOSPHOLIPID-METABOLISM AND CA-45(2+)-ACCUMULATION IN POSTHATCH CHICKEN FOREBRAIN', NEUROSCIENCE LETTERS, 194 161-164 (1995)
DOI10.1016/0304-3940(95)11749-MAuthor URL
CitationsScopus - 7Web of Science - 8
1995HEATH JW, LITTLE GJ, ROBINSON CS, SCHULMAN H, ROSTAS JAP, 'LM AND EM IMMUNOLOCALIZATION OF ALPHA-CAMPK-II AND BETA-CAMPK-II', JOURNAL OF NEUROCHEMISTRY, 65 S22-S22 (1995)
Author URL
1995SIM ATR, RATCLIFFE E, ROSTAS JAP, 'DIFFERENTIAL REGULATION OF MEMBRANE-BOUND PROTEIN PHOSPHATASES IN BRAIN', JOURNAL OF NEUROCHEMISTRY, 65 S93-S93 (1995)
Author URL
1994SIM ATR, RATCLIFFE E, MUMBY MC, VILLAMORUZZI E, ROSTAS JAP, 'DIFFERENTIAL ACTIVITIES OF PROTEIN PHOSPHATASE TYPE-1 AND TYPE-2A IN CYTOSOLIC AND PARTICULATE FRACTIONS FROM RAT FOREBRAIN', JOURNAL OF NEUROCHEMISTRY, 62 1552-1559 (1994) [C1]
Author URL
CitationsScopus - 47Web of Science - 50
1994SIM ATR, RATCLIFFE E, ROSTAS JAP, 'SELECTIVE REGULATION OF PROTEIN PHOSPHATASE-I DURING DEVELOPMENT IN CHICKEN FOREBRAIN AND CEREBELLUM', JOURNAL OF NEUROCHEMISTRY, 63 S73-S73 (1994)
Author URL
1994MARTIN H, ROSTAS J, PATEL Y, AITKEN A, 'SUBCELLULAR-LOCALIZATION OF 14-3-3-ISOFORMS IN RAT-BRAIN USING SPECIFIC ANTIBODIES', JOURNAL OF NEUROCHEMISTRY, 63 2259-2265 (1994)
Author URL
CitationsScopus - 117Web of Science - 114
1993MURRAY L, SIM ATR, ROSTAS JAP, CAPON RJ, 'ISOPALINURIN - A MILD PROTEIN PHOSPHATASE INHIBITOR FROM A SOUTHERN AUSTRALIAN MARINE SPONGE, DYSIDEA SP', AUSTRALIAN JOURNAL OF CHEMISTRY, 46 1291-1294 (1993)
Author URL
CitationsWeb of Science - 9
1993MURRAY L, SIM ATR, ROSTAS JAP, CAPON RJ, 'ISOPALINURIN - A MILD PROTEIN PHOSPHATASE INHIBITOR FROM A SOUTHERN AUSTRALIAN MARINE SPONGE, DYSIDEA SP (VOL 46, PG 1291-1294, 1993)', AUSTRALIAN JOURNAL OF CHEMISTRY, 46 1824-1824 (1993)
Author URL
1993CAPON RJ, ELSBURY K, BUTLER MS, LU CC, HOOPER JNA, ROSTAS JAP, et al., 'EXTRAORDINARY LEVELS OF CADMIUM AND ZINC IN A MARINE SPONGE, TEDANIA-CHARCOTI TOPSENT - INORGANIC CHEMICAL DEFENSE AGENTS', EXPERIENTIA, 49 263-264 (1993)
DOI10.1007/BF01923536Author URL
CitationsScopus - 21Web of Science - 21
1993SHAHIN S, BARTLETT PF, MILLAR TJ, MCLENNAN I, ROSTAS JAP, 'DISTRIBUTION OF THE SLOW CARDIAC ISOFORM OF SKELETAL-MUSCLE CA2+-ATPASE IN DEVELOPING AND MATURE TISSUES OF CHICKENS DETERMINED USING A MONOCLONAL-ANTIBODY', JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY, 41 215-224 (1993)
Author URL
1993KAVANAGH JM, BOYD TL, BUNN SJ, ROSTAS JAP, 'QUISQUALATE RECEPTOR FUNCTION IN CHICKEN FOREBRAIN', JOURNAL OF NEUROCHEMISTRY, 61 S16-S16 (1993)
Author URL
1993SIM ATR, RATCLIFFE E, DUNKLEY PR, ROSTAS JAP, 'MEMBRANE-BOUND FORMS OF PROTEIN PHOSPHATASE TYPE-1 AND TYPE-2A HAVE LOWER ACTIVITY THAN CYTOSOLIC FORMS IN BRAIN', JOURNAL OF NEUROCHEMISTRY, 61 S136-S136 (1993)
Author URL
Co-authorsPeter Dunkley
1993WANG X, ROSTAS JAP, 'MATURATION CHANGES IN CAM-PKII IN CHICKEN CEREBELLUM', JOURNAL OF NEUROCHEMISTRY, 61 S219-S219 (1993)
Author URL
1993LENGYEL I, NICHOL KA, BENNETT MR, ROSTAS JAP, DUNKLEY PR, 'PROTEIN-PHOSPHORYLATION IN CHICK CILIARY GANGLION', JOURNAL OF NEUROCHEMISTRY, 61 S221-S221 (1993)
Author URL
Co-authorsPeter Dunkley
1993KAVANAGH JM, ROSTAS JAP, 'AMPA RECEPTOR FUNCTION IN CHICKEN FOREBRAIN', JOURNAL OF NEUROCHEMISTRY, 61 S269-S269 (1993)
Author URL
1993SIM ATR, LLOYD HGE, JARVIE PE, MORRISON M, ROSTAS JAP, DUNKLEY PR, 'SYNAPTOSOMAL AMINO-ACID RELEASE - EFFECT OF INHIBITING PROTEIN PHOSPHATASES WITH OKADAIC ACID', NEUROSCIENCE LETTERS, 160 181-184 (1993)
DOI10.1016/0304-3940(93)90408-DAuthor URL
CitationsScopus - 17Web of Science - 19
Co-authorsPeter Dunkley
1992ROSTAS JAP, DUNKLEY PR, 'MULTIPLE FORMS AND DISTRIBUTION OF CALCIUM/CALMODULIN-STIMULATED PROTEIN KINASE-II IN BRAIN', JOURNAL OF NEUROCHEMISTRY, 59 1191-1202 (1992)
DOI10.1111/j.1471-4159.1992.tb08428.xAuthor URL
CitationsScopus - 40Web of Science - 49
Co-authorsPeter Dunkley
1992HEATH JW, GLENFIELD PJ, ROSTAS JAP, 'STRUCTURAL MATURATION OF SYNAPSES IN THE RAT SUPERIOR CERVICAL-GANGLION CONTINUES BEYOND 4 WEEKS OF AGE', NEUROSCIENCE LETTERS, 142 17-21 (1992)
DOI10.1016/0304-3940(92)90610-JAuthor URL
CitationsScopus - 2Web of Science - 3
1992KAVANAGH JM, POWIS DA, DODD PR, ROSTAS JAP, 'NMDA RECEPTOR FUNCTION IN CHICKEN FOREBRAIN DURING MATURATION', MOLECULAR NEUROPHARMACOLOGY, 2 193-195 (1992)
Author URL
1991WEINBERGER RP, ROSTAS JAP, 'EFFECT OF ZINC ON CALMODULIN-STIMULATED PROTEIN KINASE-II AND PROTEIN-PHOSPHORYLATION IN RAT CEREBRAL-CORTEX', JOURNAL OF NEUROCHEMISTRY, 57 605-614 (1991)
DOI10.1111/j.1471-4159.1991.tb03791.xAuthor URL
CitationsScopus - 17Web of Science - 25
1991SIM ATR, DUNKLEY PR, JARVIE PE, ROSTAS JAP, 'MODULATION OF SYNAPTOSOMAL PROTEIN-PHOSPHORYLATION DEPHOSPHORYLATION BY CALCIUM IS ANTAGONIZED BY INHIBITION OF PROTEIN PHOSPHATASES WITH OKADAIC ACID', NEUROSCIENCE LETTERS, 126 203-206 (1991)
DOI10.1016/0304-3940(91)90554-7Author URL
CitationsScopus - 19Web of Science - 29
Co-authorsPeter Dunkley
1991KAVANAGH JM, DODD PR, ROSTAS JAP, '[H-3] MK-801 BINDING IN IMMATURE AND MATURE CHICKEN FOREBRAIN', NEUROSCIENCE LETTERS, 134 83-87 (1991)
DOI10.1016/0304-3940(91)90514-TAuthor URL
CitationsWeb of Science - 13
1991Kavanagh JM, Dodd PR, Rostas JAP, '[3H]MK-801 binding in immature and mature chicken forebrain', Neuroscience Letters, 134 83-87 (1991)

Previous studies have shown that the rate of calcium uptake stimulated by N-methyl-d-aspartate (NMDA) increased during the maturation phase of synapse development in chicken forebrain. To investigate whether this change in function is due to a change in the properties of NMDA receptor associated ion channels, we measured the binding of [3H]MK-801 (a ligand which binds to the NMDA receptor associated ion channel) to membranes from immature and mature chicken brain. The binding properties of MK-801 in chicken brain were similar to those in mammalian brain. There was no significant difference in any of the binding parameters measured at the two ages, i.e. KD, Bmax and optimal glutamate concentration for and maximal enhancement by glutamate of MK-801 binding. These results suggest that there is no change in the NMDA operated ion-channels during maturation. Thus the maturational change in NMDA receptor function could be due to: a change in the agonist portion of the NMDA receptor, a change in the regulation of the receptor/ionophore complex, perhaps by the postsynaptic density whose structure and composition changes during the same period, or a change in the number of voltage-sensitive calcium channels recruited as a result of NMDA receptor activation. © 1991.

DOI10.1016/0304-3940(91)90514-T
CitationsScopus - 10
1991JEITNER TM, JARVIE PE, COSTA M, ROSTAS JAP, DUNKLEY PR, 'PROTEIN-PHOSPHORYLATION IN GUINEA-PIG MYENTERIC GANGLIA AND BRAIN - PRESENCE OF CALMODULIN KINASE-II, PROTEIN-KINASE-C AND CYCLIC-AMP KINASE AND CHARACTERIZATION OF MAJOR PHOSPHOPROTEINS', NEUROSCIENCE, 40 555-569 (1991)
DOI10.1016/0306-4522(91)90141-AAuthor URL
CitationsScopus - 8Web of Science - 14
Co-authorsPeter Dunkley
1991KOSZKA C, BRENT VA, ROSTAS JAP, 'DEVELOPMENTAL-CHANGES IN PHOSPHORYLATION OF MAP-2 AND SYNAPSIN-I IN CYTOSOL AND TAXOL POLYMERIZED MICROTUBULES FROM CHICKEN BRAIN', NEUROCHEMICAL RESEARCH, 16 637-644 (1991)
DOI10.1007/BF00965549Author URL
CitationsScopus - 8Web of Science - 8
1991ROSTAS JAP, KAVANAGH JM, DODD PR, HEATH JW, POWIS DA, 'MECHANISMS OF SYNAPTIC PLASTICITY - CHANGES IN POSTSYNAPTIC DENSITIES AND GLUTAMATE RECEPTORS IN CHICKEN FOREBRAIN DURING MATURATION', MOLECULAR NEUROBIOLOGY, 5 203-216 (1991)
DOI10.1007/BF02935546Author URL
CitationsScopus - 24Web of Science - 21
1990SHAHIN S, JEFFREY PL, ROSTAS JAP, 'THY-1-LIKE IMMUNOREACTIVITY IN DEVELOPING CHICKEN SKELETAL-MUSCLE - IDENTIFICATION OF A CROSS-REACTIVE SLOW-FIBER SPECIFIC MOLECULE THAT IS NOT THY-1', JOURNAL OF NEUROSCIENCE RESEARCH, 26 45-54 (1990)
DOI10.1002/jnr.490260106Author URL
CitationsScopus - 1Web of Science - 3
1990Wang X, Rostas JA, 'Development of Ca2(+)-calmodulin activated protein kinase II and cAMP activated protein kinase in the central nervous system', Sheng li ke xue jin zhan [Progress in physiology], 21 58-60 (1990)
1990WEBB GR, REDMAN S, HENNRIKUS D, ROSTAS JAP, SANSONFISHER RW, 'THE PREVALENCE AND SOCIODEMOGRAPHIC CORRELATES OF HIGH-RISK AND PROBLEM DRINKING AT AN INDUSTRIAL WORKSITE', BRITISH JOURNAL OF ADDICTION, 85 495-507 (1990)
Author URL
CitationsScopus - 22Web of Science - 21
Co-authorsRob Sanson-Fisher
1989SHAHIN S, LASHER RS, MILLAR TJ, ROSTAS JAP, 'A MONOCLONAL-ANTIBODY AGAINST THE SLOW ISOFORM OF SKELETAL-MUSCLE CA-2+-ATPASE SELECTIVELY STAINS A SUBPOPULATION OF NEURONS IN THE CENTRAL NERVOUS-SYSTEM', NEUROSCIENCE LETTERS, 106 163-168 (1989)
DOI10.1016/0304-3940(89)90220-6Author URL
CitationsScopus - 1Web of Science - 3
1989ROSTAS JAP, BRENT VA, SECCOMBE M, WEINBERGER RP, DUNKLEY PR, 'PURIFICATION AND CHARACTERIZATION OF CALMODULIN-STIMULATED PROTEIN KINASE-II FROM 2-DAY AND ADULT CHICKEN FOREBRAIN', JOURNAL OF MOLECULAR NEUROSCIENCE, 1 93-104 (1989)
DOI10.1007/BF02918895Author URL
CitationsScopus - 5Web of Science - 10
Co-authorsPeter Dunkley
1989Rostas JAP, Brent VA, Seccombe M, Weinberger RP, Dunkley PR, 'Purification and characterization of calmodulin-stimulated protein kinase II from two-day and adult chicken forebrain', Journal of Molecular Neuroscience, 1 93-104 (1989)

Soluble calmodulin-stimulated protein kinase II has been purified from 2-day and adult chicken forebrain At both ages the holoenzyme eluted from a Superose-6B column with an apparent molecular weight of approximately 700,000 daltons and contained three subunits. The subunits were found to be the counterparts of the alpha, beta, and betá subunits of the enzyme purified from adult rat brain in that they had one-dimensional phosphopeptide maps that were indistinguishable from those of the corresponding subunit in the rat enzyme and they migrated in SDS-polyacrylamide gels with the same apparent molecular weights. However, the doublet formed by the beta subunit was much more clearly resolved in the chicken enzyme and the betá subunit, which was much more abundant in the adult chicken than in the adult rat, was also found to be a doublet. The ratio of the concentrations of the alpha and beta subunits changed during development. By autoradiography following autophosphorylation, the alpha:beta ratios of the 2-day and adult enzymes were 0.89±0.07 and 1.92±0.26, reapectively; by silver staining the alpha:beta ratios were 0.95±0.11 and 1.85±0.17, respectively. The concentration of the betá subunit was equal to that of the beta subunit at both ages. Autophosphorylation produced a decrease in the electrophoretic mobility of the alpha and beta subunits in SDS-polyacrylamide gels and a marked decrease in the calcium dependence of the substrate phosphorylation activity of the enzyme at both ages. The purified enzyme from chicken brain appeared to be more stable under standard in vitro assay conditions than the rat enzyme, and this was particularly so for the enzyme from 2-day forebrain. © 1989 Humana Press Inc.

DOI10.1007/BF02918895
Co-authorsPeter Dunkley
1988DUNKLEY PR, HEATH JW, HARRISON SM, JARVIE PE, GLENFIELD PJ, ROSTAS JAP, 'A RAPID PERCOLL GRADIENT PROCEDURE FOR ISOLATION OF SYNAPTOSOMES DIRECTLY FROM AN S-1 FRACTION - HOMOGENEITY AND MORPHOLOGY OF SUBCELLULAR-FRACTIONS', BRAIN RESEARCH, 441 59-71 (1988)
DOI10.1016/0006-8993(88)91383-2Author URL
CitationsScopus - 274Web of Science - 315
Co-authorsPeter Dunkley
1988Weinberger RP, Rostas JA, 'Developmental changes in protein phosphorylation in chicken forebrain. I. cAMP-stimulated phosphorylation.', Brain Research, 471 249-257 (1988)

The net level of cyclic AMP-stimulated protein phosphorylation was investigated in cytosolic and membrane fractions from chicken forebrain between embryonic day 13 (E13) and 52 days post-hatching. Throughout this period the majority of the net level of cAMP-stimulated phosphorylation of endogenous proteins was in the cytosolic fractions. Between day -8 (E13) and adult, the net level of cAMP-stimulated phosphorylation of endogenous proteins in the cytosol (S3) and crude synaptic plasma membrane (P2-M) fractions fell by 3 and 4 fold, respectively, when expressed per mg protein and rose by 5 and 10 fold, respectively, when expressed per fraction. The changes in specific activity were completed by 6-15 days post-hatching. The occluded cytosol (P2-S) fraction showed little change in the net level of cAMP-stimulated phosphorylation of endogenous proteins per mg protein. Major changes in phosphoprotein patterns involving both decreases and increases in phosphorylation occurred in all fractions from day -8 (E13) to day 6 post-hatch; thereafter the phosphoprotein bands and their relative intensities were unchanged. Three bands (P90 in S3; P41 and P31 in P2-M) contained major cAMP-stimulated phosphoproteins in embryonic brain but were absent after hatching. When cAMP-stimulated phosphorylation activity was measured in S3 and P-2M using an exogenous peptide substrate (Kemptide) there was no change in kinase activity per mg protein between day -8 (E13) and 30 days post-hatch. This suggests that the decrease in the net level of cAMP stimulated phosphorylation of endogenous proteins was due to the decrease in levels of endogenous phosphoproteins rather than protein kinase activity.(ABSTRACT TRUNCATED AT 250 WORDS)

CitationsScopus - 1
1988Weinberger RP, Rostas JA, 'Developmental changes in protein phosphorylation in chicken forebrain. II. Calmodulin stimulated phosphorylation.', Brain Research, 471 259-272 (1988)

The development of calmodulin stimulated protein phosphorylation, with particular reference to calmodulin-stimulated protein kinase II (CMK II), was investigated in 3 subcellular fractions of chicken forebrain: cytosol (S3), crude synaptic plasma membranes (P2-M) and occluded cytosol (P2-S). Changes in the level of calmodulin-stimulated phosphorylation of endogenous proteins occurred over a protracted time course and were not complete until after day 52 post-hatching. By day 15 post-hatching, calmodulin-stimulated phosphoproteins characteristic of embryonic fractions had all disappeared and those characteristic of adult tissue were present but not necessarily at their mature levels. The levels of CMK II were estimated from the autophosphorylation of the alpha-subunit which was the only phosphoprotein present at 53,000 Da in the 3 fractions. Overall, calmodulin-stimulated phosphorylation and CMK II levels were low in embryonic brain and high in adult brain but two specific changes in CMK II were observed during development: (1) although CMK II concentrations increased in both membrane and cytosolic fractions until day 23 the kinase was predominantly cytoplasmic (approximately 75%) until day 23, after which it became increasingly membrane bound so that by day 52 post-hatching the majority of CMK II was present in the synaptic membrane fraction, and (2) the relative concentrations of the alpha- and beta-subunits changed from an alpha:beta-value of approximately 1:1 in the 19 day embryo to approximately 1:2 by 15 days post-hatch after which no further change was seen. The occurrence of major changes in the calmodulin stimulated protein phosphorylation system for up to 6-8 weeks after synapse formation is completed in the forebrain, provides further support for the existence of a synapse maturation phase of neuronal differentiation which is distinct from synapse formation. This phase involves only a specific subset of the developmental changes occurring in the calmodulin-stimulated phosphorylation system.

CitationsScopus - 6
1988ROSTAS JAP, SECCOMBE M, WEINBERGER RP, '2 DEVELOPMENTALLY REGULATED ISOENZYMES OF CALMODULIN-STIMULATED PROTEIN KINASE-II IN RAT FOREBRAIN', JOURNAL OF NEUROCHEMISTRY, 50 945-953 (1988)
DOI10.1111/j.1471-4159.1988.tb03003.xAuthor URL
CitationsScopus - 31Web of Science - 48
1988DUNKLEY PR, JARVIE PE, ROSTAS JAP, 'DISTRIBUTION OF CALMODULIN-STIMULATED AND CYCLIC AMP-STIMULATED PROTEIN-KINASES IN SYNAPTOSOMES', JOURNAL OF NEUROCHEMISTRY, 51 57-68 (1988)
DOI10.1111/j.1471-4159.1988.tb04835.xAuthor URL
CitationsScopus - 7Web of Science - 20
Co-authorsPeter Dunkley
1988WEINBERGER RP, ROSTAS JAP, 'DEVELOPMENTAL-CHANGES IN PROTEIN-PHOSPHORYLATION IN CHICKEN FOREBRAIN .1. CAMP-STIMULATED PHOSPHORYLATION', DEVELOPMENTAL BRAIN RESEARCH, 43 249-257 (1988)
DOI10.1016/0165-3806(88)90103-4Author URL
CitationsScopus - 6Web of Science - 11
1988WEINBERGER RP, ROSTAS JAP, 'DEVELOPMENTAL-CHANGES IN PROTEIN-PHOSPHORYLATION IN CHICKEN FOREBRAIN .2. CALMODULIN STIMULATED PHOSPHORYLATION', DEVELOPMENTAL BRAIN RESEARCH, 43 259-272 (1988)
DOI10.1016/0165-3806(88)90104-6Author URL
CitationsScopus - 10Web of Science - 19
1988DICKINSON JA, ROSTAS JAP, NICHOLS B, EBELING W, DICKESON J, MATTHEWS D, et al., 'THE USE OF COTININE TO VALIDATE SMOKING SELF-REPORT IN PRIMARY CARE', COMMUNITY HEALTH STUDIES, 12 472-473 (1988)
Author URL
1987SHELDRAKE RF, CROCKER BD, HUSBAND AJ, ROSTAS JAP, 'ISOLATION AND PURIFICATION OF A THY-1-LIKE GLYCOPROTEIN FROM SHEEP BRAIN AND ITS DISTRIBUTION IN OVINE TISSUES', RESEARCH IN VETERINARY SCIENCE, 42 358-364 (1987)
Author URL
CitationsScopus - 1Web of Science - 3
1987Rostas JAP, Brent V, Dunkley PR, 'The effect of calmodulin and autophosphorylation on the activity of calmodulin-stimulated protein kinase II', Neuroscience Research Communications, 1 3-8 (1987)
CitationsScopus - 2
Co-authorsPeter Dunkley
1986DUNKLEY PR, JARVIE PE, HEATH JW, KIDD GJ, ROSTAS JAP, 'A RAPID METHOD FOR ISOLATION OF SYNAPTOSOMES ON PERCOLL GRADIENTS', BRAIN RESEARCH, 372 115-129 (1986)
DOI10.1016/0006-8993(86)91464-2Author URL
CitationsScopus - 272Web of Science - 366
Co-authorsPeter Dunkley
1986Sedman GL, Jeffrey PL, Austin L, Rostas JA, 'The metabolic turnover of the major proteins of the postsynaptic density.', Brain Research, 387 221-230 (1986)

We have used the method of Austin, Lowry, Brown and Carter, to measure the steady-state metabolic half-life of tubulin (alpha and beta individually) and actin (beta and gamma together) in the total cytosolic (S3), microsomal (P3), synaptic plasma membrane (SPM) and synaptic junction (SJ) subcellular fractions from 6-day-old and adult chicken forebrain. In the SPM and SJ fractions we also measured the steady-state metabolic half-life of the major postsynaptic density protein (mPSDp). In SPM and SJ fractions from 6-day-old chickens tubulin and actin turned over approximately twice as slowly (t1/2 approximately equal to 24 days) as tubulin and actin in the S3 fraction (t1/2 approximately equal to 13 days). This difference was unlikely merely to be due to association with membranes since the t1/2 values for the proteins were the same in P3 and S3. The estimated t1/2 values for mPSDp were similar to that for tubulin and actin in SPM and SJ fractions. Similar results were obtained in adult chickens except that all t1/2 values in all fractions were approximately 30% larger. The calculated t1/2 values did not change between labelling periods of 4 and 6.5 h suggesting that the lag phase of incorporation of newly synthesized PSD proteins is sufficiently rapid to not produce this result artefactually. When the brain from a non-labelled chicken was homogenized in the presence of the S3 fraction from a labelled chicken and sub-fractionated the relative specific activities of the SPM and SJ fractions produced were 1-2% of those from the labelled brain. These results support the notion that tubulin and actin are intrinsic components of the PSD.

CitationsScopus - 6
1986Weinberger RP, Rostas JA, 'Subcellular distribution of a calmodulin-dependent protein kinase activity in rat cerebral cortex during development.', Brain research, 394 37-50 (1986)

The postnatal development of calmodulin-stimulated phosphorylation of endogenous proteins, in particular the autophosphorylated subunits of the calmodulin-stimulated protein kinase II, were investigated in subcellular fractions of rat cerebral cortex. The major subunit had a mol. wt. of 53,000 Da (designated 50 kDa) and the minor one a mol. wt. of 63,000 Da (designated 60 kDa). The 50-kDa subunit was found to be the only significant phosphoprotein in each fraction and throughout development at its molecular weight. However, the 60-kDa subunit was found to comigrate with other phosphoproteins that accounted for up to 15% of the radioactivity at this molecular weight and which differed between the fractions. 50-kDa autophosphorylation was found to be 3-fold greater in cytoplasmic fractions at day 10 and by adults was evenly distributed between membrane and cytoplasmic fractions. A similar pattern was also found for the total calmodulin-stimulated phosphorylation. Changes in autophosphorylation activity of the 50-kDa subunit were found to represent changes in kinase activity rather than alterations in phosphatase activity. In the membrane, this change was shown to be due to changes in the amount of enzyme. Although in the adult autophosphorylation activity is evenly distributed between membrane and soluble fractions, when differences in phosphatase activity and lack of autophosphorylation activity of the majority of post-synaptic density-associated kinase is taken into account, it is clear that the vast majority of the enzyme is membrane-bound. Phosphorylation of endogenous substrates paralleled the development of 50-kDa subunit autophosphorylation, most of which occurred between day 14 and day 30, a period which follows the most rapid phase of synaptogenesis. This pattern was different from that of the phosphorylation of myelin basic protein and two substrates of the calcium-phospholipid-dependent protein kinase. There was also a change in the ratio of autophosphorylation activity of the 50-kDa and 60-kDa subunits during development which appears to be due to a change in the amount of the subunits themselves. This ratio was the same in all fractions at any one age. We suggest that this change is due to the existence of at least two developmentally regulated isoenzymes in the cortex.

CitationsScopus - 3
1986LEUNG WN, JEFFREY PL, ROSTAS JAP, 'EFFECT OF DENERVATION ON SARCOLEMMAL PROTEINS AND GLYCOPROTEINS OF FAST AND SLOW MAMMALIAN SKELETAL-MUSCLE', EXPERIMENTAL NEUROLOGY, 91 229-245 (1986)
DOI10.1016/0014-4886(86)90064-6Author URL
CitationsScopus - 4Web of Science - 11
1986ROSTAS JAP, WEINBERGER RP, DUNKLEY PR, 'MULTIPLE POOLS AND MULTIPLE FORMS OF CALMODULIN-STIMULATED PROTEIN-KINASE DURING DEVELOPMENT - RELATIONSHIP TO POSTSYNAPTIC DENSITIES', PROGRESS IN BRAIN RESEARCH, 69 355-371 (1986)
DOI10.1016/S0079-6123(08)61070-5Author URL
CitationsScopus - 10Web of Science - 26
Co-authorsPeter Dunkley
1986WEINBERGER RP, ROSTAS JAP, 'SUBCELLULAR-DISTRIBUTION OF A CALMODULIN-DEPENDENT PROTEIN-KINASE ACTIVITY IN RAT CEREBRAL-CORTEX DURING DEVELOPMENT', DEVELOPMENTAL BRAIN RESEARCH, 29 37-50 (1986)
DOI10.1016/0165-3806(86)90080-5Author URL
CitationsScopus - 12Web of Science - 32
1986SEDMAN GL, JEFFREY PL, AUSTIN L, ROSTAS JAP, 'THE METABOLIC TURNOVER OF THE MAJOR PROTEINS OF THE POSTSYNAPTIC DENSITY', MOLECULAR BRAIN RESEARCH, 1 221-230 (1986)
DOI10.1016/0169-328X(86)90028-8Author URL
CitationsScopus - 3Web of Science - 10
1986ROSTAS JAP, BRENT VA, HEATH JW, NEAME RLB, POWIS DA, WEINBERGER RP, DUNKLEY PR, 'THE SUBCELLULAR-DISTRIBUTION OF A MEMBRANE-BOUND CALMODULIN-STIMULATED PROTEIN-KINASE', NEUROCHEMICAL RESEARCH, 11 253-268 (1986)
DOI10.1007/BF00967973Author URL
CitationsScopus - 11Web of Science - 23
Co-authorsPeter Dunkley
1985JEFFREY PL, LEUNG WN, ROSTAS JAP, 'THE EFFECT OF TETRODOTOXIN ON MAMMALIAN SARCOLEMMAL PROTEINS AND GLYCOPROTEINS', BIOCHEMISTRY INTERNATIONAL, 10 715-722 (1985)
Author URL
1984LEUNG WN, JEFFREY PL, ROSTAS JAP, 'THE EFFECT OF DENERVATION ON MAMMALIAN SARCOLEMMAL PROTEINS AND GLYCOPROTEINS', MUSCLE & NERVE, 7 35-49 (1984)
DOI10.1002/mus.880070107Author URL
CitationsScopus - 4Web of Science - 12
1984ROSTAS JAP, BRENT VA, GULDNER FH, 'THE MATURATION OF POST-SYNAPTIC DENSITIES IN CHICKEN FOREBRAIN', NEUROSCIENCE LETTERS, 45 297-304 (1984)
DOI10.1016/0304-3940(84)90242-8Author URL
CitationsScopus - 14Web of Science - 24
1984SEDMAN GL, JEFFREY PL, ROSTAS JAP, 'THE TURNOVER RATES OF CHICK BRAIN SYNAPTIC JUNCTION PROTEINS', PROCEEDINGS OF THE AUSTRALIAN BIOCHEMICAL SOCIETY, 16 32-32 (1984)
Author URL
1984WEINBERGER RP, DUNKLEY PR, ROSTAS JAP, 'DEVELOPMENTAL ACCUMULATION OF A CALMODULIN STIMULATED PROTEIN-KINASE IN SYNAPTIC-MEMBRANES', PROCEEDINGS OF THE AUSTRALIAN BIOCHEMICAL SOCIETY, 16 87-87 (1984)
Author URL
CitationsWeb of Science - 1
Co-authorsPeter Dunkley
1983ROSTAS JAP, SHEVENAN TA, SINCLAIR CM, JEFFREY PL, 'THE PURIFICATION AND CHARACTERIZATION OF A THY-1-LIKE GLYCOPROTEIN FROM CHICKEN BRAIN', BIOCHEMICAL JOURNAL, 213 143-152 (1983)
Author URL
CitationsScopus - 23Web of Science - 41
1983ROSTAS JAP, BRENT VA, DUNKLEY PR, 'THE MAJOR CALMODULIN-STIMULATED PHOSPHOPROTEIN OF SYNAPTIC JUNCTIONS AND THE MAJOR POST-SYNAPTIC DENSITY PROTEIN ARE DISTINCT', NEUROSCIENCE LETTERS, 43 161-165 (1983)
DOI10.1016/0304-3940(83)90181-7Author URL
CitationsScopus - 5Web of Science - 18
Co-authorsPeter Dunkley
1982LEUNG WN, JEFFREY PL, ROSTAS JAP, 'DENERVATION EXPOSES CRYPTIC CONCANAVALIN A BINDING-SITES IN SKELETAL-MUSCLE MEMBRANES', NEUROSCIENCE LETTERS, 30 31-36 (1982)
DOI10.1016/0304-3940(82)90007-6Author URL
CitationsScopus - 3Web of Science - 8
1981LEUNG WN, ROSTAS JAP, JEFFREY PL, 'PLASTICITY OF SKELETAL-MUSCLE MEMBRANES IN FOLLOWING DENERVATION', PROCEEDINGS OF THE AUSTRALIAN BIOCHEMICAL SOCIETY, 14 118-118 (1981)
Author URL
1981ROSTAS JAP, LEUNG WN, JEFFREY PL, 'GLYCOSYLTRANSFERASE ACTIVITIES IN CHICKEN BRAIN SYNAPTIC JUNCTIONS', NEUROSCIENCE LETTERS, 24 155-160 (1981)
DOI10.1016/0304-3940(81)90240-8Author URL
CitationsScopus - 3Web of Science - 6
1981ROSTAS JAP, JEFFREY PL, 'MATURATION OF SYNAPSES IN CHICKEN FOREBRAIN', NEUROSCIENCE LETTERS, 25 299-304 (1981)
DOI10.1016/0304-3940(81)90408-0Author URL
CitationsScopus - 8Web of Science - 16
1980ROSTAS JAP, SHEVENAN TA, JEFFREY PL, 'A DEVELOPMENTALLY REGULATED THY-1-LIKE MEMBRANE GLYCOPROTEIN IN CHICKENS', PROCEEDINGS OF THE AUSTRALIAN BIOCHEMICAL SOCIETY, 13 119-119 (1980)
Author URL
CitationsWeb of Science - 1
1979ROSTAS JAP, KELLY PT, PESIN RH, COTMAN CW, 'PROTEIN AND GLYCOPROTEIN COMPOSITION OF SYNAPTIC JUNCTIONS PREPARED FROM DISCRETE SYNAPTIC REGIONS AND DIFFERENT SPECIES', BRAIN RESEARCH, 168 151-167 (1979)
DOI10.1016/0006-8993(79)90133-1Author URL
CitationsScopus - 27Web of Science - 67
1979ROSTAS JAP, AUSTIN L, JEFFREY PL, 'SELECTIVE LABELING OF 2 PHASES OF AXONAL-TRANSPORT OF CHOLESTEROL IN THE CHICK OPTIC-SYSTEM', JOURNAL OF NEUROCHEMISTRY, 32 1461-1466 (1979)
DOI10.1111/j.1471-4159.1979.tb11085.xAuthor URL
CitationsScopus - 3Web of Science - 7
1979LEUNG WN, ROSTAS JAP, JEFFREY PL, 'ALTERATIONS IN SURFACE-MEMBRANES OF MAMMALIAN SKELETAL-MUSCLE FOLLOWING DENERVATION', PROCEEDINGS OF THE AUSTRALIAN BIOCHEMICAL SOCIETY, 12 93-93 (1979)
Author URL
1979JEFFREY PL, LEUNG WN, ROSTAS JAP, 'DENERVATION ALTERATIONS IN SURFACE-MEMBRANES OF MAMMALIAN SKELETAL-MUSCLE', MUSCLE & NERVE, 2 310-310 (1979)
Author URL
1977ROSTAS JAP, KELLY PT, COTMAN CW, 'IDENTIFICATION OF MEMBRANE GLYCO-COMPONENTS IN POLYACRYLAMIDE GELS - RAPID METHOD USING I-125-LABELED LECTINS', ANALYTICAL BIOCHEMISTRY, 80 366-372 (1977)
DOI10.1016/0003-2697(77)90657-1Author URL
CitationsScopus - 14Web of Science - 48
1977HOWE PRC, FENWICK EM, ROSTAS JAP, LIVETT BG, 'IMMUNOCHEMICAL COMPARISON OF SYNAPTIC PLASMA-MEMBRANE AND SYNAPTIC VESICLE MEMBRANE ANTIGENS', JOURNAL OF NEUROCYTOLOGY, 6 339-352 (1977)
DOI10.1007/BF01175195Author URL
CitationsScopus - 3Web of Science - 10
Co-authorsPeter Howe
1977ROSTAS JAP, JEFFREY PL, 'IMMUNOCHEMICAL CHARACTERIZATION OF SYNAPTOSOMAL MEMBRANE ANTIGENS FROM CHICKEN BRAIN - HISTOCHEMICAL LOCALIZATION IN DAY-OLD CHICK', NEUROCHEMICAL RESEARCH, 2 59-85 (1977)
DOI10.1007/BF00966022Author URL
CitationsScopus - 1Web of Science - 8
1977ROSTAS JAP, JEFFREY PL, 'IMMUNOCHEMICAL CHARACTERIZATION OF SYNAPTOSOMAL MEMBRANE ANTIGENS FROM CHICKEN BRAIN - HISTOCHEMICAL DEMONSTRATION OF CHANGES IN DISTRIBUTION DURING DEVELOPMENT', NEUROCHEMICAL RESEARCH, 2 171-189 (1977)
DOI10.1007/BF00964095Author URL
CitationsScopus - 2Web of Science - 3
1977ROSTAS JAP, JEFFREY PL, 'IMMUNOCHEMICAL CHARACTERIZATION OF SYNAPTOSOMAL MEMBRANE ANTIGENS FROM CHICKEN BRAIN - QUANTITATIVE-DETERMINATION AND PARTIAL CHARACTERIZATION', NEUROCHEMICAL RESEARCH, 2 395-406 (1977)
DOI10.1007/BF00965463Author URL
CitationsWeb of Science - 1
1975ROSTAS JAP, MCGREGOR A, JEFFREY PL, 'TRANSPORT OF CHOLESTEROL IN CHICK OPTIC-SYSTEM', JOURNAL OF NEUROCHEMISTRY, 24 295-302 (1975)
DOI10.1111/j.1471-4159.1975.tb11879.xAuthor URL
CitationsScopus - 8Web of Science - 14
1975HOWE PRC, ROSTAS JAP, FENWICK EM, LIVETT BG, 'FURTHER EVIDENCE FOR INCORPORATION OF SYNAPTIC VESICLE PROTEINS INTO SYNAPTOSOMAL PLASMA-MEMBRANES', PROCEEDINGS OF THE AUSTRALIAN BIOCHEMICAL SOCIETY, 8 95-95 (1975)
Author URL
CitationsWeb of Science - 1
Co-authorsPeter Howe
1975ROSTAS JAP, JEFFREY PL, 'ANTIGENIC MEMBRANE COMPONENTS IN DEVELOPING NERVE-CELLS', PROCEEDINGS OF THE AUSTRALIAN BIOCHEMICAL SOCIETY, 8 117-117 (1975)
Author URL
1975ROSTAS JAP, JEFFREY PL, 'RESTRICTED MOBILITY OF NEURONAL MEMBRANE ANTIGENS', NEUROSCIENCE LETTERS, 1 47-53 (1975)
DOI10.1016/0304-3940(75)90011-7Author URL
CitationsWeb of Science - 4
1974LIVETT BG, ROSTAS JAP, JEFFREY PL, AUSTIN L, 'ANTIGENICITY OF ISOLATED SYNAPTOSOMAL MEMBRANES', EXPERIMENTAL NEUROLOGY, 43 330-338 (1974)
DOI10.1016/0014-4886(74)90174-5Author URL
CitationsScopus - 12Web of Science - 24
1974ROSTAS JAP, MCGREGOR A, JEFFREY PL, AUSTIN L, 'CHOLESTEROL TRANSPORT IN CRANIAL AND PERIPHERAL-NERVES', PROCEEDINGS OF THE AUSTRALIAN BIOCHEMICAL SOCIETY, 7 35-35 (1974)
Author URL
1973ROSTAS JAP, JEFFREY PL, 'UNUSUAL DISRUPTION OF SYNAPTOSOMES BY FRENCH PRESS TREATMENT', PROCEEDINGS OF THE AUSTRALIAN BIOCHEMICAL SOCIETY, 6 46-46 (1973)
Author URL
Show 135 more journal articles

Conference (31 outputs)

YearCitationAltmetricsLink
2013Skelding KA, Majeed ABA, Carpenter H, Dickson PW, Rostas JA, 'Can functional outcomes of CaMKII double phosphorylation be predicted from outcomes following single phosphorylation of CaMKII?', JOURNAL OF NEUROCHEMISTRY, Cancun, MEXICO (2013) [E3]
Author URL
Co-authorsKathryn Skelding, Phil Dickson
2012Skelding KA, Abdul Majeed ABB, Dickson PW, Spratt NJ, Rostas JA, 'CAMKII is regulated differently in brains regions with differing sensitivities to ischaemia/excitotoxicity', Abstracts. Australian Neuroscience Society 32nd Annual Meeting, Gold Coast, Queensland (2012) [E3]
Co-authorsPhil Dickson, Kathryn Skelding, Neil Spratt
2012Skelding KA, Dickson PW, Verrills NM, Rostas JA, 'Dephosphorylation of CAMKII at T253 controls progression through metaphase', Abstracts. Australian Neuroscience Society 32nd Annual Meeting, Gold Coast, Queensland (2012) [E3]
Co-authorsNikki Verrills, Phil Dickson, Kathryn Skelding
2012Abdul Majeed ABB, Skelding KA, Dickson PW, Rostas JA, 'Does phosphorylation of CaMKII at multiple sites regulate CaMKII targeting co-operatively or independently?', Abstracts. Australian Neuroscience Society 32nd Annual Meeting, Gold Coast, Queensland (2012) [E3]
Co-authorsPhil Dickson, Kathryn Skelding
2012Rostas JA, Skelding KA, Fluechter L, Dickson PW, Spratt NJ, 'CaMKII is Differentially Regulated in Striatum and Cortex', JOURNAL OF NEUROCHEMISTRY (2012) [E3]
Author URL
Co-authorsPhil Dickson, Neil Spratt, Kathryn Skelding
2012Rostas JA, Skelding KA, Fluechter L, Dickson PW, Spratt NJ, 'CaMKII is differentially regulated in striatum and cortex', Journal of Molecular Neuroscience: Abstracts The 21st Annual Meeting of the Israel Society for Neuroscience & The First Binational Australian-Israeli Meeting in Neuroscience, Eilat, Israel (2012) [E3]
Co-authorsPhil Dickson, Neil Spratt, Kathryn Skelding
2011Skelding KA, Chung S, Pepperall D-G, Tomkins AJ, Spratt NJ, Rostas JA, 'The role of CaMKII in neuronal sensitivity to ischaemia', Oral abstracts. Australian Neuroscience Society Annual Meeting, Auckland, NZ (2011) [E3]
Co-authorsKathryn Skelding, Neil Spratt
2010Rostas JA, Skelding KA, 'The role of targeting in the regulation of CaMKII in vivo', OzBio 2010: The Molecules of Life - from Discovery to Biotechnology Symposium Abstracts, Melbourne, Vic (2010) [E3]
Co-authorsKathryn Skelding
2010Skelding KA, Tomkins AJ, Fluechter L, Pepperall D-G, Spratt NJ, Rostas JA, 'Ischaemia-induced CaMKII phosphorylation in hypertensive and normotensive rats', Proceding of the Australian Neuroscience Society, Sydney, NSW (2010) [E3]
Co-authorsNeil Spratt, Kathryn Skelding
2010Skelding KA, Verrills NM, Dickson PW, Rostas JA, 'Regulation of proliferation of neuroblastoma cells by CaMKII', Proceding of the Australian Neuroscience Society, Sydney, NSW (2010) [E3]
Co-authorsNikki Verrills, Kathryn Skelding, Phil Dickson
2010Skelding KA, Xue J, Suzuki T, Verrills NM, Dickson PW, Rostas JA, 'Mechanisms of phosphorylation-sensitive CaMKII targeting', Proceding of the Australian Neuroscience Society, Sydney, NSW (2010) [E3]
Co-authorsPhil Dickson, Nikki Verrills, Kathryn Skelding
2009Skelding KA, Liao X, Verrills NM, Fluechter L, Sim AT, Dickson PW, Rostas JA, 'Functional consequences of CaMKII phosphorylation at THR253 in neurons', ANS 2009 Abstracts: Posters, Canberra, ACT (2009) [E3]
Co-authorsPhil Dickson, Alistair Sim, Nikki Verrills, Kathryn Skelding
2009Tomkins AJ, Rostas JA, Pepperall D-G, Calford MB, Spratt NJ, 'Infarction occurs more rapidly in hypertensive rats', ANS 2009 Abstracts: Posters, Canberra, ACT (2009) [E3]
Co-authorsNeil Spratt
2009Rostas JA, 'Regulation of CaMKII activity', ANS 2009 Abstracts: Symposia, Canberra, ACT (2009) [E3]
2009Rostas JA, Skelding KA, Liao X, Verrills NM, Dickson PW, 'Regulation of CaMKII by targeting', Proceedings of the 2nd Australia-China Biomedical Research Conference, - (2009) [E3]
Co-authorsKathryn Skelding, Nikki Verrills, Phil Dickson
2009Skelding KA, Liao X, Verrills NM, Fluechter L, Dickson PW, Rostas JA, 'CaMKII phosphorylation at T253 alters neuronal growth rates and morphology', Journal of Neurochemistry, Busan, Korea (2009) [E3]
DOI10.1111/j.1471-4159.2009.06238.x
Co-authorsKathryn Skelding, Nikki Verrills, Phil Dickson
2009Rostas JA, Skelding KA, Verrills NM, Suzuki PW, Dickson T, 'CaMKII binding partners vary with cell type and phosphorylation state', Journal of Neurochemistry, Busan, Korea (2009) [E3]
DOI10.1111/j.1471-4159.2009.06238.x
Co-authorsKathryn Skelding, Nikki Verrills
2008Skelding KA, Verrills NM, Fluechter L, Sim AT, Dickson PW, Rostas JA, 'Identification of CaMKII binding proteins in brain sensitive to CaMKII phosphorylation state', Proceedings of the Australian Neuroscience Society, Hobart, TAS (2008) [E3]
Co-authorsKathryn Skelding, Phil Dickson, Nikki Verrills, Alistair Sim
2008Skelding KA, Verrills NM, Fluechter L, Sim AT, Dickson PW, Rostas JA, 'Development of a novel method for the identification of CaMKII binding proteins', Journal of Neurochemistry, Shanghai, China (2008) [E3]
Co-authorsNikki Verrills, Phil Dickson, Alistair Sim, Kathryn Skelding
2007Hunter M, Atkinson RJ, Rostas JA, 'Perturbed maturation in chicken forebrain via the induction of mild hypothyroidism: developmental changes measured by auditory event related potentials (Poster)', 7th IBRO 2007 World Congress of Neuroscience Program, Melbourne (2007) [E3]
Co-authorsMick Hunter
2007Figliola A, Serrano E, Rostas JA, Hunter M, Rosso OA, 'Study of EEG brain maturation signals with multifractal detrended fluctuation analysis', Nonequilibrium Statistical Mechanics and Nonlinear Physics: XV Conference on Nonequilibrium Statistical Mechanics and Nonlinear Physics. AIP Conference Proceedings, Mar del Plato, Argentina (2007) [E1]
DOI10.1063/1.2746746
CitationsScopus - 3Web of Science - 1
Co-authorsMick Hunter
2007Fernandez JG, Larrondo HA, Figliola A, Serrano E, Rostas JA, Hunter M, Rosso OA, 'Brain maturation changes characterized by algorithmic complexity (lempel and ziv complexity)', Nonequilibrium Statistical Mechanics and Nonlinear Physics: XV Conference on Nonequilibrium Statistical Mechanics and Nonlinear Physics. AIP Conference Proceedings, Mar del Plata, Argentina (2007) [E1]
CitationsScopus - 3Web of Science - 5
Co-authorsMick Hunter
2006Rostas JAP, Migues P, Lehmann IT, Fluechter L, Cammarota M, Gurd JW, et al., 'Phosphorylation of CAMKII at Thr253 occurs in vivo and enhances binding to isolated post synaptic densities', JOURNAL OF NEUROCHEMISTRY, Singapore, SINGAPORE (2006)
Author URL
Co-authorsPhil Dickson
2006Rostas JA, Migues PV, Fluechter L, Dickson PW, Sim AT, Rawoff S, et al., 'Phosphorylation of CAMKII at T253 induced by transient global ischaemia', Proceedings of the Australian Neuroscience Society, Sydney (2006) [E3]
Co-authorsPhil Dickson, Alistair Sim
2006Atkinson RJ, Thomas (Duff) L, Migues PV, Fluechter L, Rostas JA, Hunter M, 'Perturbed maturation in the chicken forebrain by the induction of late onset mild hypo-thyroidsim', Proceedings of the Australian Neuroscience Society, Sydney (2006) [E3]
Co-authorsMick Hunter
2005Migues PV, Lehmann IT, Fluechter L, Cammarota M, Sim AT, Gurd JW, et al., 'Phosphorylation of CaMKII on T253 enhances its association with postsynaptic densities', JOURNAL OF NEUROCHEMISTRY, Innsbruck, AUSTRIA (2005)
Author URL
Co-authorsPhil Dickson
2005Rostas JA, 'New mechanisms of control of CaMKII: Regulating the regulator', JOURNAL OF NEUROCHEMISTRY, Innsbruck, AUSTRIA (2005)
Author URL
2005Atkinson RJ, Migues PV, Fluechter L, Hunter M, Rostas JA, 'Biochemical characterisation of maturational changes in components of glutamatergic synapses in the chick IMHV', Australian Neuroscience Society: Proceedings of the 25th Annual, Perth, Australia (2005) [E3]
Co-authorsMick Hunter
2004Rostas JA, Baldwin ML, Cammarota MP, Sim AT, 'The regulation of synaptosomal exocytosis by Src tyrosine kinases', Journal of Neurochemistry, Hong Kong (2004) [E3]
Co-authorsAlistair Sim
2004Migues PV, Sim AT, Rostas JA, 'Changes in KCI-induced phosphorylation of AMPA and NMDA receptors during chicken brain maturation', Journal of Neurochemistry, Hong Kong (2004) [E3]
Co-authorsAlistair Sim
2003Migues PV, Cammarota M, Fluechter L, Bissoon N, Gurd JW, Rostas JAP, 'A new phosphorylation site on CaMKII in vivo', JOURNAL OF NEUROCHEMISTRY, WANCHAI, PEOPLES R CHINA (2003)
Author URL
Show 28 more conferences
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Grants and Funding

Summary

Number of grants85
Total funding$9,729,934

Click on a grant title below to expand the full details for that specific grant.


20133 grants / $68,096

Central Contribution to Faculty Peer Review - FHM$41,500

Funding body: University of Newcastle

Funding bodyUniversity of Newcastle
Project TeamEmeritus Professor John Rostas
SchemeSpecial Project Grant
RoleLead
Funding Start2013
Funding Finish2013
GNoG1301329
Type Of FundingInternal
CategoryINTE
UONY

International Society for Neurochemistry - American Society for Neurochemsity Biennial Conference, Cancun Mexico, 20 - 24 April 2013$2,000

Funding body: University of Newcastle - Faculty of Health and Medicine

Funding bodyUniversity of Newcastle - Faculty of Health and Medicine
Project TeamEmeritus Professor John Rostas
SchemeTravel Grant
RoleLead
Funding Start2013
Funding Finish2013
GNoG1300537
Type Of FundingInternal
CategoryINTE
UONY

20124 grants / $58,000

Role of CaMKII targeting in neuronal susceptibility to excitotoxic cell death$20,000

Funding body: University of Newcastle

Funding bodyUniversity of Newcastle
Project TeamEmeritus Professor John Rostas, Doctor Neil Spratt, Associate Professor Phillip Dickson, Doctor Nikki Verrills
SchemeNear Miss Grant
RoleLead
Funding Start2012
Funding Finish2012
GNoG1200673
Type Of FundingInternal
CategoryINTE
UONY

Regulation of the cell cycle by phosphorylation dependent targeting of CaMKII$20,000

Funding body: University of Newcastle

Funding bodyUniversity of Newcastle
Project TeamDoctor Nikki Verrills, Doctor Kathryn Skelding, Emeritus Professor John Rostas, Associate Professor Phillip Dickson, Conjoint Professor Keith Jones
SchemeNear Miss Grant
RoleInvestigator
Funding Start2012
Funding Finish2012
GNoG1200679
Type Of FundingInternal
CategoryINTE
UONY

FT13 Future Fellowship External Applicant Support - FH$16,000

Funding body: University of Newcastle

Funding bodyUniversity of Newcastle
Project TeamEmeritus Professor John Rostas
SchemeSpecial Project Grant
RoleLead
Funding Start2012
Funding Finish2012
GNoG1201092
Type Of FundingInternal
CategoryINTE
UONY

Asian Pacific Society for Neurochemistry/Japanese Society for Neurochemistry, Kobe, Japan, 30 September - 2 October 2012$2,000

Funding body: University of Newcastle - Faculty of Health and Medicine

Funding bodyUniversity of Newcastle - Faculty of Health and Medicine
Project TeamEmeritus Professor John Rostas
SchemeTravel Grant
RoleLead
Funding Start2012
Funding Finish2012
GNoG1201003
Type Of FundingInternal
CategoryINTE
UONY

20115 grants / $80,200

Regulation of Breast Cancer Growth by a Novel Phosphorylation-Dependent Targeting Mechanism$35,000

Funding body: Hunter Medical Research Institute

Funding bodyHunter Medical Research Institute
Project TeamDoctor Kathryn Skelding, Doctor Nikki Verrills, Emeritus Professor John Rostas
SchemeBreast Cancer Project Grant
RoleInvestigator
Funding Start2011
Funding Finish2011
GNoG1001005
Type Of FundingContract - Aust Non Government
Category3AFC
UONY

Role of CaMKII targeting in stroke susceptibility and outcome$18,200

Funding body: Hunter Medical Research Institute

Funding bodyHunter Medical Research Institute
Project TeamEmeritus Professor John Rostas, Doctor Neil Spratt, Doctor Kathryn Skelding
SchemeStroke Research Project Grant
RoleLead
Funding Start2011
Funding Finish2011
GNoG1001013
Type Of FundingContract - Aust Non Government
Category3AFC
UONY

Role of CaMKII targeting in stroke susceptibility and outcome$15,000

Funding body: Brain Foundation (NSW Branch)

Funding bodyBrain Foundation (NSW Branch)
Project TeamDoctor Neil Spratt, Emeritus Professor John Rostas
SchemeResearch Grant
RoleInvestigator
Funding Start2011
Funding Finish2011
GNoG1000716
Type Of FundingGrant - Aust Non Government
Category3AFG
UONY

IMPLEN NanoPhotometer pearl$10,000

Funding body: NHMRC (National Health & Medical Research Council)

Funding bodyNHMRC (National Health & Medical Research Council)
Project TeamConjoint Associate Professor Murray Cairns, Associate Professor Paul Tooney, Professor Alan Brichta, Emeritus Professor John Rostas, Emeritus Professor Patricia Michie, Conjoint Professor Keith Jones, Professor Ulli Schall, Associate Professor Phillip Dickson, Doctor Frederick Walker, Doctor Rick Thorne, Doctor Chris Dayas, Doctor Nikki Verrills, Doctor Janet Holt, Doctor Severine Roselli, Doctor Kathryn Skelding, Doctor Jude Weidenhofer, Associate Professor Liz Milward, Doctor Charles De Bock, Doctor Julie Merriman-Jones, Doctor Jing Qin Wu, Doctor Bing Liu, Mr Dan Johnstone, Ms Belinda Goldie, Doctor Natalie Beveridge
SchemeEquipment Grant
RoleInvestigator
Funding Start2011
Funding Finish2011
GNoG1100030
Type Of FundingOther Public Sector - Commonwealth
Category2OPC
UONY

23rd Biennial Meeting of International Society for Neurochemistry joint with the European Society for Neurochemistry, Athens, Greece, 28/8/2011 - 1/9/2011$2,000

Funding body: University of Newcastle - Faculty of Health and Medicine

Funding bodyUniversity of Newcastle - Faculty of Health and Medicine
Project TeamEmeritus Professor John Rostas
SchemeTravel Grant
RoleLead
Funding Start2011
Funding Finish2011
GNoG1100698
Type Of FundingInternal
CategoryINTE
UONY

20104 grants / $542,900

Molecular and cellular characterisation of schizophrenia associated dysfunction in microRNA biogenesis$478,500

Funding body: NHMRC (National Health & Medical Research Council)

Funding bodyNHMRC (National Health & Medical Research Council)
Project TeamConjoint Associate Professor Murray Cairns, Professor Rodney Scott, Associate Professor Paul Tooney, Emeritus Professor John Rostas, Professor Alan Brichta
SchemeProject Grant
RoleInvestigator
Funding Start2010
Funding Finish2010
GNoG0190196
Type Of FundingAust Competitive - Commonwealth
Category1CS
UONY

ABI 7500 Real Time PCR System $34,000

Funding body: NHMRC (National Health & Medical Research Council)

Waters, Ultra high performance liquid chromatography system (nano-Aquity) $30,000

Funding body: NHMRC (National Health & Medical Research Council)

Funding bodyNHMRC (National Health & Medical Research Council)
Project TeamDoctor Mark Baker, Laureate Professor John Aitken, Professor Adam McCluskey, Associate Professor Brett Nixon, Emeritus Professor John Rostas, Professor Eileen McLaughlin
SchemeEquipment Grant
RoleInvestigator
Funding Start2010
Funding Finish2010
GNoG1000051
Type Of FundingOther Public Sector - Commonwealth
Category2OPC
UONY

OzBio2010 - The Molecules of Life, Melbourne Convention & Exhibition Centre, 26 September 2010 - 1 October 2010$400

Funding body: University of Newcastle - Faculty of Health and Medicine

Funding bodyUniversity of Newcastle - Faculty of Health and Medicine
Project TeamEmeritus Professor John Rostas
SchemeTravel Grant
RoleLead
Funding Start2010
Funding Finish2010
GNoG1000742
Type Of FundingInternal
CategoryINTE
UONY

20094 grants / $777,775

China-Australia therapeutic hypothermia in stroke (CATHS) research program: identification of diagnostic molecular markers and therapeutic targets involved in re-warming related brain injury$230,275

Funding body: NSW Office for Science & Medical Research

Funding bodyNSW Office for Science & Medical Research
Project TeamConjoint Professor Chris Levi, Doctor Mark Baker, Doctor Neil Spratt, Emeritus Professor John Rostas
SchemeChina-NSW Collaborative Research Program
RoleInvestigator
Funding Start2009
Funding Finish2009
GNoG0190392
Type Of FundingOther Public Sector - State
Category2OPS
UONY

22nd Biennial Meeting of the International Society for Neurochemistry, Busan, South Korea, 23-28 August 2009$2,500

Funding body: University of Newcastle - Faculty of Health and Medicine

Funding bodyUniversity of Newcastle - Faculty of Health and Medicine
Project TeamEmeritus Professor John Rostas
SchemeTravel Grant
RoleLead
Funding Start2009
Funding Finish2009
GNoG0190449
Type Of FundingInternal
CategoryINTE
UONY

20083 grants / $27,891

Is CaMKII autophosphorylation a mechanism of endogenous neuroprotection after stroke?$14,417

Funding body: University of Newcastle

Funding bodyUniversity of Newcastle
Project TeamEmeritus Professor John Rostas, Doctor Neil Spratt
SchemePilot Grant
RoleLead
Funding Start2008
Funding Finish2008
GNoG0189110
Type Of FundingInternal
CategoryINTE
UONY

Hypoxic culture chamber and low-pressure gas regulators$11,500

Funding body: NHMRC (National Health & Medical Research Council)

Funding bodyNHMRC (National Health & Medical Research Council)
Project TeamDoctor Neil Spratt, Emeritus Professor John Rostas, Professor Mike Calford
SchemeEquipment Grant
RoleInvestigator
Funding Start2008
Funding Finish2008
GNoG0188544
Type Of FundingOther Public Sector - Commonwealth
Category2OPC
UONY

8th Biennial Meetingof the Asian-Pacific Society for Neurochemistry, Shanghai, 23/6/2008 - 26/6/2008$1,974

Funding body: University of Newcastle

Funding bodyUniversity of Newcastle
Project TeamEmeritus Professor John Rostas
SchemeTravel Grant
RoleLead
Funding Start2008
Funding Finish2008
GNoG0189136
Type Of FundingInternal
CategoryINTE
UONY

20073 grants / $541,631

Functional Characterisation of a New Regulatory Mechanism for CaMKII at Synapses in Vivo$526,500

Funding body: NHMRC (National Health & Medical Research Council)

Funding bodyNHMRC (National Health & Medical Research Council)
Project TeamEmeritus Professor John Rostas, Associate Professor Phillip Dickson, Professor Alistair Sim
SchemeProject Grant
RoleLead
Funding Start2007
Funding Finish2007
GNoG0186415
Type Of FundingAust Competitive - Commonwealth
Category1CS
UONY

RQF Support for Assistant Dean (Research)$14,000

Funding body: University of Newcastle

Funding bodyUniversity of Newcastle
Project TeamEmeritus Professor John Rostas
SchemeSpecial Project Grant
RoleLead
Funding Start2007
Funding Finish2007
GNoG0187947
Type Of FundingInternal
CategoryINTE
UONY

Brain Mechanisms, Cognition and Behaviour in Birds (official satellite to IBRO World Congress of Neuroscience, Melbourne, 12/7/2007 - 17/7/2007$1,131

Funding body: University of Newcastle

Funding bodyUniversity of Newcastle
Project TeamEmeritus Professor John Rostas
SchemeTravel Grant
RoleLead
Funding Start2007
Funding Finish2007
GNoG0187938
Type Of FundingInternal
CategoryINTE
UONY

20065 grants / $1,167,920

PRC - Priority Research Centre for Brain & Mental Health Research (CBMHR)$524,282

Funding body: University of Newcastle

Funding bodyUniversity of Newcastle
Project TeamConjoint Professor Chris Levi, Professor Amanda Baker, Professor Mike Calford, Professor Trevor Day, Emeritus Professor Peter Dunkley, Professor Michael Hazelton, Professor Andrew Heathcote, Professor Brian Kelly, Conjoint Professor Vaughan Carr, Emeritus Professor Patricia Michie, Professor David Pow, Emeritus Professor John Rostas, Professor Ulli Schall, Professor Alistair Sim, Professor Mike Startup
SchemePriority Research Centre
RoleInvestigator
Funding Start2006
Funding Finish2006
GNoG0186947
Type Of FundingInternal
CategoryINTE
UONY

Functional Characterisation of CaMKII Phosphorylate at a New Site in vivo$20,000

Funding body: University of Newcastle

Funding bodyUniversity of Newcastle
Project TeamEmeritus Professor John Rostas, Associate Professor Phillip Dickson, Professor Alistair Sim
SchemeNear Miss Grant
RoleLead
Funding Start2006
Funding Finish2006
GNoG0186059
Type Of FundingInternal
CategoryINTE
UONY

7th Biennial Meeting of the Asian Pacific Society for Neurochemistry (APSN 2006), 2-5 July 2006$1,500

Funding body: University of Newcastle

Funding bodyUniversity of Newcastle
Project TeamEmeritus Professor John Rostas
SchemeTravel Grant
RoleLead
Funding Start2006
Funding Finish2006
GNoG0186659
Type Of FundingInternal
CategoryINTE
UONY

BioRad Mini-PROTEAN3 Electrophoresis system$995

Funding body: Hunter Medical Research Institute

Funding bodyHunter Medical Research Institute
Project TeamEmeritus Professor Leonie Ashman, Associate Professor Phillip Dickson, Emeritus Professor Peter Dunkley, Emeritus Professor John Rostas, Professor Judith Scott, Professor Alistair Sim, Doctor Nikki Verrills
SchemeEquipment Grant
RoleInvestigator
Funding Start2006
Funding Finish2006
GNoG0187280
Type Of FundingNot Known
CategoryUNKN
UONY

20054 grants / $239,689

The Australian EEG Database - Infrastructure Support$20,000

Funding body: Hunter Medical Research Institute

Funding bodyHunter Medical Research Institute
Project TeamEmeritus Professor Patricia Michie, Conjoint Associate Professor Mick Hunter, Emeritus Professor John Rostas, Conjoint Associate Professor David Williams, Professor Pablo Moscato, Associate Professor Frans Henskens
SchemeHMRI Brain and Mental Health Research Program
RoleInvestigator
Funding Start2005
Funding Finish2005
GNoG0185719
Type Of FundingNot Known
CategoryUNKN
UONY

Mechanisms and functional consequences of glutamate receptor maturation in chicken brain and New ways of controlling CaMKII in cells$10,000

Funding body: University of Newcastle

Funding bodyUniversity of Newcastle
Project TeamEmeritus Professor John Rostas, Professor Ulli Schall
SchemeProject Grant
RoleLead
Funding Start2005
Funding Finish2005
GNoG0184707
Type Of FundingInternal
CategoryINTE
UONY

20th Biennial International Society for Neurochemistry (ISN) and European Society for Neurochemistry (ESN) Conference, 21-26 August 2005$2,500

Funding body: University of Newcastle

Funding bodyUniversity of Newcastle
Project TeamEmeritus Professor John Rostas
SchemeTravel Grant
RoleLead
Funding Start2005
Funding Finish2005
GNoG0185425
Type Of FundingInternal
CategoryINTE
UONY

20043 grants / $583,491

Differential regulation of two modes of exocytosis by protein phosphatases$393,750

Funding body: NHMRC (National Health & Medical Research Council)

Funding bodyNHMRC (National Health & Medical Research Council)
Project TeamProfessor Alistair Sim, Emeritus Professor John Rostas
SchemeProject Grant
RoleInvestigator
Funding Start2004
Funding Finish2004
GNoG0182930
Type Of FundingAust Competitive - Commonwealth
Category1CS
UONY

Biacore3000-Expansion of Proteomics Facility$187,341

Funding body: ARC (Australian Research Council)

Funding bodyARC (Australian Research Council)
Project TeamProfessor Alistair Sim, Laureate Professor John Aitken, Emeritus Professor Peter Dunkley, Emeritus Professor John Rostas, Associate Professor Phillip Dickson, Emeritus Professor Leonie Ashman, Professor Roger Smith, Professor Gordon Burns, Professor Adam McCluskey, Associate Professor Paul Tooney, Dr Fraser Ross, Conjoint Professor Ray Rose
SchemeLinkage Infrastructure Equipment & Facilities (LIEF)
RoleInvestigator
Funding Start2004
Funding Finish2004
GNoG0183030
Type Of FundingScheme excluded from IGS
CategoryEXCL
UONY

12th International Conference on Second Messengers and Phosphoproteins, 3-7 August 2004, Canada$2,400

Funding body: University of Newcastle

Funding bodyUniversity of Newcastle
Project TeamEmeritus Professor John Rostas
SchemeTravel Grant
RoleLead
Funding Start2004
Funding Finish2004
GNoG0184474
Type Of FundingInternal
CategoryINTE
UONY

20034 grants / $547,000

The Emlyn and Jennie Thomas Fund for Postgraduate Sholarships in Cardiovascular Health.$500,000

Funding body: Hunter Medical Research Institute

Funding bodyHunter Medical Research Institute
Project TeamEmeritus Professor John Rostas
SchemeResearch Grant
RoleLead
Funding Start2003
Funding Finish2003
GNoG0182686
Type Of FundingContract - Aust Non Government
Category3AFC
UONY

Quantitative EEG analysis of the maturational changes associated with childhood and juvenile absence epilepsy.$28,000

Funding body: John Hunter Children`s Hospital Research Foundation

Funding bodyJohn Hunter Children`s Hospital Research Foundation
Project TeamDoctor Robert Smith, Conjoint Associate Professor Mick Hunter, Emeritus Professor John Rostas
SchemeResearch Grant (Defunct)
RoleInvestigator
Funding Start2003
Funding Finish2003
GNoG0181850
Type Of FundingOther Public Sector - State
Category2OPS
UONY

New Control Mechanism for CaMKII in Nerve Cells: regulating the regulator.$13,000

Funding body: University of Newcastle

Funding bodyUniversity of Newcastle
Project TeamEmeritus Professor John Rostas
SchemeProject Grant
RoleLead
Funding Start2003
Funding Finish2003
GNoG0182499
Type Of FundingInternal
CategoryINTE
UONY

Characterisation of the Pre-pulse Inhibition learning paradigm in chickens$6,000

Funding body: University of Newcastle

Funding bodyUniversity of Newcastle
Project TeamConjoint Associate Professor Mick Hunter, Emeritus Professor John Rostas
SchemeProject Grant
RoleInvestigator
Funding Start2003
Funding Finish2003
GNoG0182457
Type Of FundingInternal
CategoryINTE
UONY

20024 grants / $923,647

Proteomic and Genomic Analysis Facility.$473,332

Funding body: Wellcome Trust

Funding bodyWellcome Trust
Project TeamProfessor Alistair Sim, Emeritus Professor Peter Dunkley, Emeritus Professor John Rostas, Professor Rodney Scott, Emeritus Professor Leonie Ashman
SchemeMajor Equipment Award
RoleInvestigator
Funding Start2002
Funding Finish2002
GNoG0181527
Type Of FundingScheme excluded from IGS
CategoryEXCL
UONY

Mechanisms of Glutamate Receptor Maturation in Chicken Brain$417,000

Funding body: NHMRC (National Health & Medical Research Council)

Funding bodyNHMRC (National Health & Medical Research Council)
Project TeamEmeritus Professor John Rostas, Dr Martin Cammarota, Professor Alistair Sim, Conjoint Associate Professor Mick Hunter, Professor Ulli Schall
SchemeProject Grant
RoleLead
Funding Start2002
Funding Finish2002
GNoG0180913
Type Of FundingAust Competitive - Commonwealth
Category1CS
UONY

Peltier Thermal Cycler and associated equipment essential for establishing cDNA Retroviral Libraries$21,315

Funding body: NHMRC (National Health & Medical Research Council)

Funding bodyNHMRC (National Health & Medical Research Council)
Project TeamEmeritus Professor Leonie Ashman, Dr Petranel Ferrao, Professor Gordon Burns, Professor Alistair Sim, Emeritus Professor Peter Dunkley, Emeritus Professor John Rostas, Associate Professor Phillip Dickson, Professor Dirk Van Helden, Dr Perry Hartfield
SchemeEquipment Grant
RoleInvestigator
Funding Start2002
Funding Finish2002
GNoG0181919
Type Of FundingAust Competitive - Commonwealth
Category1CS
UONY

Biochemical characerization of the DTAL learning task: comparison between immature and mature chickens$12,000

Funding body: University of Newcastle

Funding bodyUniversity of Newcastle
Project TeamEmeritus Professor John Rostas, Conjoint Associate Professor Mick Hunter
SchemeProject Grant
RoleLead
Funding Start2002
Funding Finish2002
GNoG0181301
Type Of FundingInternal
CategoryINTE
UONY

20013 grants / $2,117,500

HMRI Infrastructure Block Grant.$2,100,000

Funding body: Hunter Medical Research Institute

Funding bodyHunter Medical Research Institute
Project TeamEmeritus Professor John Rostas
SchemeNSW Dept Health - Infrastructure Funding
RoleLead
Funding Start2001
Funding Finish2001
GNoG0180712
Type Of FundingContract - Aust Non Government
Category3AFC
UONY

Reciprocal Regulation of Kinases and Phosphatases in Long Term Synaptic Plasticity$15,000

Funding body: University of Newcastle

Funding bodyUniversity of Newcastle
Project TeamEmeritus Professor John Rostas, Professor Alistair Sim, Professor Robert Callister
SchemeProject Grant
RoleLead
Funding Start2001
Funding Finish2001
GNoG0180078
Type Of FundingInternal
CategoryINTE
UONY

Joint Meeting of ISN & ASN & Satellite, Argentina 26 August - 4 September 2001$2,500

Funding body: University of Newcastle

Funding bodyUniversity of Newcastle
Project TeamEmeritus Professor John Rostas
SchemeTravel Grant
RoleLead
Funding Start2001
Funding Finish2001
GNoG0181432
Type Of FundingInternal
CategoryINTE
UONY

20003 grants / $44,500

Neurolucida Imaging System.$24,000

Funding body: NHMRC (National Health & Medical Research Council)

Funding bodyNHMRC (National Health & Medical Research Council)
Project TeamConjoint Professor Loris Chahl, Associate Professor Paul Tooney, Emeritus Professor Peter Dunkley, Emeritus Professor John Rostas
SchemeEquipment Grant
RoleInvestigator
Funding Start2000
Funding Finish2000
GNoG0179167
Type Of FundingAust Competitive - Commonwealth
Category1CS
UONY

Regulating the Regulator: Using Immunochemical Tools to Reveal How CaMPK-II is Controlled by Phosporylation In Vivo.$13,500

Funding body: University of Newcastle

Funding bodyUniversity of Newcastle
Project TeamEmeritus Professor John Rostas, Emeritus Professor Peter Dunkley, Dr Martin Cammarota, Dr Sabine Fieuw-Makaroff
SchemeProject Grant
RoleLead
Funding Start2000
Funding Finish2000
GNoG0178869
Type Of FundingInternal
CategoryINTE
UONY

On the mechanisms involved in learning and memory in the mature chicken brain.$7,000

Funding body: University of Newcastle

Funding bodyUniversity of Newcastle
Project TeamConjoint Associate Professor Mick Hunter, Emeritus Professor John Rostas
SchemeProject Grant
RoleInvestigator
Funding Start2000
Funding Finish2000
GNoG0178909
Type Of FundingInternal
CategoryINTE
UONY

19996 grants / $254,007

Confocal Laser Scanning Microscope Facility.$203,107

Funding body: ARC (Australian Research Council)

Funding bodyARC (Australian Research Council)
Project TeamLaureate Professor John Aitken, Emeritus Professor John Patrick, Conjoint Professor Christina Offler, Associate Professor David McCurdy, Conjoint Professor Ray Rose, Doctor Tim Roberts, Conjoint Associate Professor Russell Jones, Doctor John Clulow, Emeritus Professor John Rostas, Professor Alistair Sim, Professor Gordon Burns, Conjoint Professor Loris Chahl, DR DAVID KAY
SchemeLarge Equipment Grant
RoleInvestigator
Funding Start1999
Funding Finish1999
GNoG0177945
Type Of FundingScheme excluded from IGS
CategoryEXCL
UONY

FPLC Protein Purification System$16,000

Funding body: NHMRC (National Health & Medical Research Council)

Funding bodyNHMRC (National Health & Medical Research Council)
Project TeamProfessor Alistair Sim, Emeritus Professor John Rostas, Emeritus Professor Peter Dunkley, Associate Professor Phillip Dickson
SchemeEquipment Grant
RoleInvestigator
Funding Start1999
Funding Finish1999
GNoG0180790
Type Of FundingAust Competitive - Commonwealth
Category1CS
UONY

Characterisation of giant mossy fibre-like nerve terminal in chicken hippocampus.$12,500

Funding body: ARC (Australian Research Council)

Funding bodyARC (Australian Research Council)
Project TeamEmeritus Professor John Rostas
SchemeSmall Grant
RoleLead
Funding Start1999
Funding Finish1999
GNoG0178123
Type Of FundingScheme excluded from IGS
CategoryEXCL
UONY

Development of a Versatile, Sensitive, Non-Isotopoic Method for Detecting Phosphorylated and Glycosylated Serines in Proteins and Peptides$10,000

Funding body: University of Newcastle

Funding bodyUniversity of Newcastle
Project TeamEmeritus Professor John Rostas, Professor Adam McCluskey
SchemeCollaborative Research Grant
RoleLead
Funding Start1999
Funding Finish1999
GNoG0178350
Type Of FundingInternal
CategoryINTE
UONY

RMC Collab Partner.Development of a Versatile, Sensitive, Non-Isotopic Method for Detecting Phosphorylated and Glucosylated Serines in Proteins & Peptides.$10,000

Funding body: Bio-Rad Laboratories Pty Ltd (Australia)

Funding bodyBio-Rad Laboratories Pty Ltd (Australia)
Project TeamEmeritus Professor John Rostas, Professor Adam McCluskey
SchemeResearch Grant
RoleLead
Funding Start1999
Funding Finish1999
GNoG0178764
Type Of FundingContract - Aust Non Government
Category3AFC
UONY

Joint Meeting of the International & European Societies for Neurochemistry, Berlin, Germany.$2,400

Funding body: University of Newcastle

Funding bodyUniversity of Newcastle
Project TeamEmeritus Professor John Rostas
SchemeTravel Grant
RoleLead
Funding Start1999
Funding Finish1999
GNoG0180516
Type Of FundingInternal
CategoryINTE
UONY

19985 grants / $403,890

Protein phosphorylation events in long term potentiation$339,775

Funding body: NHMRC (National Health & Medical Research Council)

Funding bodyNHMRC (National Health & Medical Research Council)
Project TeamEmeritus Professor John Rostas
SchemeProject Grant
RoleLead
Funding Start1998
Funding Finish1998
GNoG0177187
Type Of FundingAust Competitive - Commonwealth
Category1CS
UONY

Molecular Dynamics Storm 860 Tri-mode Analysis System$35,000

Funding body: Ramaciotti Foundations

Funding bodyRamaciotti Foundations
Project TeamProfessor Alistair Sim, Emeritus Professor John Rostas, Emeritus Professor Peter Dunkley, Dr Stephen Bunn, Dr Tat Cheah, Conjoint Professor Kenneth Beagley, Conjoint Professor Ray Rose, Professor Judith Scott, Doctor Cheng Chan, Professor Roger Smith
SchemeResearch Grant
RoleInvestigator
Funding Start1998
Funding Finish1998
GNoG0177453
Type Of FundingAust Competitive - Non Commonwealth
Category1NS
UONY

Investigation of giant mossy fibre-like nerve terminals in chicken hippocampus.$14,000

Funding body: ARC (Australian Research Council)

Funding bodyARC (Australian Research Council)
Project TeamEmeritus Professor John Rostas
SchemeSmall Grant
RoleLead
Funding Start1998
Funding Finish1998
GNoG0177356
Type Of FundingScheme excluded from IGS
CategoryEXCL
UONY

Pharmaco-behavioural study of changes in the biochemical pathways involved in learning and memory in immature and mature chicken brain.$14,000

Funding body: University of Newcastle

Funding bodyUniversity of Newcastle
Project TeamConjoint Associate Professor Mick Hunter, Emeritus Professor John Rostas
SchemeProject Grant
RoleInvestigator
Funding Start1998
Funding Finish1998
GNoG0177408
Type Of FundingInternal
CategoryINTE
UONY

16th International Australasian Winter Conference on Brain Research and the Satellite Symposium on Learning and Memory Mechanisms$1,115

Funding body: University of Newcastle

Funding bodyUniversity of Newcastle
Project TeamEmeritus Professor John Rostas
SchemeTravel Grant
RoleLead
Funding Start1998
Funding Finish1998
GNoG0180590
Type Of FundingInternal
CategoryINTE
UONY

19974 grants / $187,958

Characteristation of Novel Molecular Probes for Protein Phosphatases.$160,000

Funding body: ARC (Australian Research Council)

Funding bodyARC (Australian Research Council)
Project TeamProfessor Alistair Sim, Emeritus Professor John Rostas, Dr R Capon
SchemeLarge Grant
RoleInvestigator
Funding Start1997
Funding Finish1997
GNoG0176188
Type Of FundingAust Competitive - Commonwealth
Category1CS
UONY

Application of a new non-isotopic techniequ to measure changes in protein phosphorylation in vivo.$15,000

Funding body: University of Newcastle

Funding bodyUniversity of Newcastle
Project TeamEmeritus Professor John Rostas
SchemeProject Grant
RoleLead
Funding Start1997
Funding Finish1997
GNoG0177494
Type Of FundingInternal
CategoryINTE
UONY

Molecular mechanisms underlying presynaptic long-term potentiation in hippocampal synaptosomes.$10,000

Funding body: ARC (Australian Research Council)

Funding bodyARC (Australian Research Council)
Project TeamImre Lengyel, Emeritus Professor John Rostas
SchemeSmall Grant
RoleInvestigator
Funding Start1997
Funding Finish1997
GNoG0176720
Type Of FundingScheme excluded from IGS
CategoryEXCL
UONY

Joint Meeting of the International Society for Neurochemistry and the American Society for Neurochemistry, Boston, USA, 20-26 July 1997$2,958

Funding body: University of Newcastle

Funding bodyUniversity of Newcastle
Project TeamEmeritus Professor John Rostas
SchemeTravel Grant
RoleLead
Funding Start1997
Funding Finish1997
GNoG0179556
Type Of FundingInternal
CategoryINTE
UONY

19963 grants / $25,120

Establishment of techniques for preparing genetically engineered molecules to study the regulation of neuronal and cellular function$12,000

Funding body: University of Newcastle

Funding bodyUniversity of Newcastle
Project TeamEmeritus Professor John Rostas, Emeritus Professor Peter Dunkley, Professor Robert Callister
SchemeProject Grant
RoleLead
Funding Start1996
Funding Finish1996
GNoG0175744
Type Of FundingInternal
CategoryINTE
UONY

Combined biochemical and electrophysiological investigations of the molecular mechanisms responsible for synaptic plasticity$11,620

Funding body: Ramaciotti Foundations

Funding bodyRamaciotti Foundations
Project TeamEmeritus Professor John Rostas
SchemeResearch Grant
RoleLead
Funding Start1996
Funding Finish1996
GNoG0175557
Type Of FundingAust Competitive - Non Commonwealth
Category1NS
UONY

Avian Brain and Behaviour Meeting, Tihany, Hungary, 25-28 August 1996$1,500

Funding body: University of Newcastle

Funding bodyUniversity of Newcastle
Project TeamEmeritus Professor John Rostas
SchemeTravel Grant
RoleLead
Funding Start1996
Funding Finish1996
GNoG0176529
Type Of FundingInternal
CategoryINTE
UONY

19955 grants / $352,386

Subcellular pools of CaMPK-II and protein phosphorylation: Role in long term potentiation.$312,026

Funding body: NHMRC (National Health & Medical Research Council)

Funding bodyNHMRC (National Health & Medical Research Council)
Project TeamEmeritus Professor John Rostas, Conjoint Associate Professor John Heath
SchemeProject Grant
RoleLead
Funding Start1995
Funding Finish1995
GNoG0174555
Type Of FundingAust Competitive - Commonwealth
Category1CS
UONY

95APP. High resolution purification of intra-cellular signalling proteins from brain by FPLC.$20,000

Funding body: Ramaciotti Foundations

Funding bodyRamaciotti Foundations
Project TeamProfessor Alistair Sim, Emeritus Professor Peter Dunkley, Emeritus Professor John Rostas
SchemeResearch Grant
RoleInvestigator
Funding Start1995
Funding Finish1995
GNoG0174696
Type Of FundingAust Competitive - Non Commonwealth
Category1NS
UONY

Establishment of new model systems and techniques for studying neurotransmision$9,000

Funding body: University of Newcastle

Funding bodyUniversity of Newcastle
Project TeamEmeritus Professor Peter Dunkley, Emeritus Professor John Rostas, Dr B Walmsley
SchemeProject Grant
RoleInvestigator
Funding Start1995
Funding Finish1995
GNoG0174902
Type Of FundingInternal
CategoryINTE
UONY

Phosphorylation of glycoprotein GP-180:- Implications for NMDA-type glutamate receptor function$9,000

Funding body: University of Newcastle

Funding bodyUniversity of Newcastle
Project TeamProfessor Alistair Sim, Emeritus Professor John Rostas
SchemeProject Grant
RoleInvestigator
Funding Start1995
Funding Finish1995
GNoG0174918
Type Of FundingInternal
CategoryINTE
UONY

15th Biennial Meeting of the International Society for Neurochemistry (Kyoto, Japan)$2,360

Funding body: University of Newcastle

Funding bodyUniversity of Newcastle
Project TeamEmeritus Professor John Rostas
SchemeTravel Grant
RoleLead
Funding Start1995
Funding Finish1995
GNoG0175540
Type Of FundingInternal
CategoryINTE
UONY

19944 grants / $41,000

Mechanism of Nitric Oxide Modulation of Secretion in Autonomic Ganglia$22,500

Funding body: National Heart Foundation of Australia

Funding bodyNational Heart Foundation of Australia
Project TeamEmeritus Professor Peter Dunkley, Emeritus Professor John Rostas
SchemeGrant-In-Aid
RoleInvestigator
Funding Start1994
Funding Finish1994
GNoG0175235
Type Of FundingAust Competitive - Non Commonwealth
Category1NS
UONY

Establishment of new model systems and techniques for studying neurotransmission$13,500

Funding body: ARC (Australian Research Council)

Funding bodyARC (Australian Research Council)
Project TeamEmeritus Professor John Rostas, Emeritus Professor Peter Dunkley, Dr B Walmsley
SchemeSmall Grant
RoleLead
Funding Start1994
Funding Finish1994
GNoG0174822
Type Of FundingScheme excluded from IGS
CategoryEXCL
UONY

'Physiological & Biochemical Basis of Brain Activity', St. Petersburg, Russia, 22 - 24 June 1994$2,500

Funding body: University of Newcastle

Funding bodyUniversity of Newcastle
Project TeamEmeritus Professor John Rostas
SchemeTravel Grant
RoleLead
Funding Start1994
Funding Finish1994
GNoG0174519
Type Of FundingInternal
CategoryINTE
UONY

Physiological & Biochemical Basis of Brain Activity - St Petersburg - 22-24 June 1994$2,500

Funding body: University of Newcastle

Funding bodyUniversity of Newcastle
Project TeamEmeritus Professor John Rostas
SchemeTravel Grant
RoleLead
Funding Start1994
Funding Finish1994
GNoG0175126
Type Of FundingInternal
CategoryINTE
UONY

19932 grants / $120,730

Advance Warning Of Cyanobacterial Activity$119,637

Funding body: Urban Water Research Association

Funding bodyUrban Water Research Association
Project TeamProfessor Alistair Sim, Emeritus Professor John Rostas
SchemeResearch Grant
RoleInvestigator
Funding Start1993
Funding Finish1993
GNoG0173334
Type Of FundingOther Public Sector - Commonwealth
Category2OPC
UONY

'14th Biennial Meeting of the Int. Soc. for Neurochemistry', Montpellier, France, 22 - 27 August 1993$1,093

Funding body: University of Newcastle

Funding bodyUniversity of Newcastle
Project TeamEmeritus Professor John Rostas
SchemeTravel Grant
RoleLead
Funding Start1993
Funding Finish1993
GNoG0174413
Type Of FundingInternal
CategoryINTE
UONY

19922 grants / $487,489

Molecular Mechanisms Of Neuronal Maturation: A Model Of Synaptic Plasticity$304,483

Funding body: NHMRC (National Health & Medical Research Council)

Funding bodyNHMRC (National Health & Medical Research Council)
Project TeamEmeritus Professor John Rostas, Conjoint Associate Professor John Heath
SchemeProject Grant
RoleLead
Funding Start1992
Funding Finish1992
GNoG0174272
Type Of FundingAust Competitive - Commonwealth
Category1CS
UONY

92,93,94GRANT. The Regulation Of Protein Phosphatases In The Brain$183,006

Funding body: NHMRC (National Health & Medical Research Council)

Funding bodyNHMRC (National Health & Medical Research Council)
Project TeamProfessor Alistair Sim, Emeritus Professor Peter Dunkley, Emeritus Professor John Rostas
SchemeProject Grant
RoleInvestigator
Funding Start1992
Funding Finish1992
GNoG0174274
Type Of FundingAust Competitive - Commonwealth
Category1CS
UONY

19912 grants / $137,114

Molecular Mechanisms Of Neuronal Maturation$99,562

Funding body: NHMRC (National Health & Medical Research Council)

Funding bodyNHMRC (National Health & Medical Research Council)
Project TeamEmeritus Professor John Rostas
SchemeProject Grant
RoleLead
Funding Start1991
Funding Finish1991
GNoG0173956
Type Of FundingAust Competitive - Commonwealth
Category1CS
UONY

Beta Scintillation Spectrometer$37,552

Funding body: NHMRC (National Health & Medical Research Council)

Funding bodyNHMRC (National Health & Medical Research Council)
Project TeamConjoint Professor David Powis, Emeritus Professor Peter Dunkley, Emeritus Professor John Rostas, Emeritus Professor Anthony Smith
SchemeEquipment Grant
RoleInvestigator
Funding Start1991
Funding Finish1991
GNoG0173965
Type Of FundingAust Competitive - Commonwealth
Category1CS
UONY
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Research Supervision

Past Supervision

YearResearch Title / Program / Supervisor Type
2013Effects of Multi-site Phosphorylation on CaMKII Function
Medical Science, Faculty of Health and Medicine
Principal Supervisor
2008Brain Maturation in Chickens: Biochemical, Behavioural and Electrophysiological Investigations
Psychology, Faculty of Science and Information Technology
Co-Supervisor
2004Differential Regulation of Two Modes of Synaptic Vesicle Exocytosis by Protein Phosphorylation
Medical Science, Faculty of Health and Medicine
Co-Supervisor
2000Identification of brain proteins whose phosphorylation is regulated by NO and cGMP
Biochemistry & Cell Biology, University of Newcastle
Principal Supervisor
1998Synaptic plasticity in chicken hippocampus.
Biochemistry & Cell Biology, University of Newcastle
Principal Supervisor
1996Mechanisms responsible for neuronal compensation following unilateral labarynthectomy in the guinea pig.
Behavioural Science N.E.C., Unknown
Co-Supervisor
1996. Changes in glutamate receptors in chicken forebrain during maturation.
Biochemistry & Cell Biology, University of Newcastle
Principal Supervisor
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Emeritus Professor John Rostas

Position

Emeritus Professor
Faculty of Health and Medicine

Contact Details

Emailjohn.rostas@newcastle.edu.au
Phone(02) 4921 5615
Fax(02) 4921 7903

Office

RoomLS3-40
BuildingLife Sciences
LocationCallaghan
University Drive
Callaghan, NSW 2308
Australia
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