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Dr John Fitter

Laboratory and Research Manager

School of Medicine and Public Health

Career Summary

Qualifications

  • PhD, University of Newcastle
  • Bachelor of Science (Honours), University of Newcastle
  • Advanced Certificate in Aircraft Maint Eng (Elect), Royal Australian Air Force - RTO
  • Diploma of Business (Frontline Management), GE Consultancy Pty Ltd (RTO)

Keywords

  • development, endocrinology, metamorphosis
  • phenotypic plasticity
  • post graduate training curriculum
  • techniques for teaching

Fields of Research

CodeDescriptionPercentage
060199Biochemistry and Cell Biology not elsewhere classified35
060799Plant Biology not elsewhere classified65

Professional Experience

UON Appointment

DatesTitleOrganisation / Department
10/02/2006 - 21/11/2006LecturerUniversity of Newcastle
Centre for Teaching and Learning
Australia

Academic appointment

DatesTitleOrganisation / Department
1/04/1993 - 1/06/1995ARC Research AssociateUniversity of Wollongong
Department of Biological Sciences
Australia

Invitations

Participant

YearTitle / Rationale
2004Sessional Teaching program
Organisation: University of Newcastle Description: Tips and techniques for sessional teachers program and subsequent video production now on-line via university intranet
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Publications

For publications that are currently unpublished or in-press, details are shown in italics.


Chapter (2 outputs)

YearCitationAltmetricsLink
2006Rose RJ, Fitter JT, McCurdy DW, Sheahan MB, 'Mitochondrial Somatic Genetics and Homeotic Effects on Flower Morphology', Floriculture, Ornamental and Plant Biotechnology: advances and topical issues, Global Science Books, Isleworth, United Kingdom 210-215 (2006) [B1]
Co-authorsDavid Mccurdy, Ray Rose
2000Smith R, Changs E-C, Mesiano S, Nicholson R, Cheng Y-H, Bowman M, et al., 'Regulation of parturition: The role of CRH', Hormones and Women's Health The Reproductive Years, Harwood Academic Publishers, Amsterdam, The Netherlands 215-219 (2000) [B1]
Co-authorsVicki Clifton, Gemma Madsen, Maria Bowman, Roger Smith

Journal article (10 outputs)

YearCitationAltmetricsLink
2014Chai SY, Smith R, Fitter JT, Mitchell C, Pan X, Ilicic M, et al., 'Increased progesterone receptor a expression in labouring human myometrium is associated with decreased promoter occupancy by the histone demethylase JARID1A', Molecular Human Reproduction, 20 442-453 (2014) [C1]

Progesterone regulates female reproductive function predominantly through two nuclear progesterone receptors (PRs), PR-A and PR-B. During human parturition myometrial PR expression is altered to favour PR-A, which activates pro-labour genes. We have previously identified histone H3 lysine 4 trimethylation (H3K4me3) as an activator of myometrial PR-A expression at labour. To further elucidate the mechanisms regulating PR isoform expression in the human uterus at labour, we have (i) determined the methylation profile of the cytosine-guanine dinucleotides (CpG) island in the promoter region of the PR gene and (ii) identified the histone-modifying enzymes that target the H3K4me3 mark at the PR promoters in term and preterm human myometrial tissues obtained before and after labour onset. Bisulphite sequencing showed that despite overall low levels of PR CpG island methylation, there was a significant decrease in methylated CpGs with labour in both preterm (P < 0.05) and term (P < 0.01) groups downstream of the PR-B transcription start site. This methylation change was not associated with altered PR-B expression, but may contribute to the increase in PR-A expression with labour. Chromatin immunoprecipitation revealed that the histone methyltransferase, SET and MYND domain-containing protein 3 (SMYD3), bound to the PR gene at significantly higher levels at the PR-A promoter compared with the PR-B promoter (P < 0.010), with no labour-associated changes observed. The H3K4 demethylase, Jumonji AT-rich interactive domain 1A (JARID1A), also bound to the PR-A, but not to the PR-B promoter prior to term labour, and decreased significantly at the onset of labour (P = 0.014), providing a mechanism for the previously reported increase in H3K4me3 level and PR-A expression with labour. Our studies suggest that epigenetic changes mediated by JARID1A, SMYD3 and DNA methylation may be responsible, at least in part, for the functional progesterone withdrawal that precipitates human labour. © The Author 2014. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved.

DOI10.1093/molehr/gau005
CitationsScopus - 2Web of Science - 2
Co-authorsGemma Madsen, Roger Smith
2005Fitter JT, Thomas MR, Niu C, Rose RJ, 'Investigation of Nicotiana tabacum (plus) N suaveolens cybrids with carpelloid stamens', Journal of Plant Physiology, 162 225-235 (2005) [C1]
DOI10.1016/j.jplph.2004.02.006
CitationsScopus - 14Web of Science - 11
Co-authorsRay Rose
2002Mesiano S, Chan EC, Fitter JT, Kwek K, Yeo G, Smith R, 'Progesterone withdrawal and estrogen activation in human parturition are coordinated by progesterone receptor-A expression in the myometrium', Journal of Clinical Endocrinology and Metabolism, 87 2924-2930 (2002) [C1]
CitationsScopus - 194Web of Science - 175
Co-authorsRoger Smith
2000West NP, Jungnitz H, Fitter JT, McArthur JD, Guzman CA, Walker MJ, 'Role of phosphoglucomutase of Bordetella bronchiseptica in lipopolysaccharide biosynthesis and virulence', INFECTION AND IMMUNITY, 68 4673-4680 (2000)
DOI10.1128/IAI.68.8.4673-4680.2000Author URL
CitationsScopus - 33Web of Science - 30
2000Cheng Y, Nicholson RC, King BR, Chan EC, Fitter JT, Smith R, 'Corticotropin-Releasing Hormone Gene Expresssion in Primary Placental Cells is Modulated by Cyclic Adenosine 3',5'-Monophosphate', The Journal of Clinical Endocrinology & Metabolism, 85 1239-1244 (2000) [C1]
CitationsScopus - 33Web of Science - 34
Co-authorsRoger Smith, Bruce King
2000Cheng Y, Nicholson RC, King BR, Chan EC, Fitter JT, Smith R, 'Glucocorticoid stimulation of Corticiotrophin-releasing hormone gene expression requires a Cyclic Adenosine 3',5'-Monophosphate regulatory element in human primary Placental Cytotrophoblast Cells', The journal of Clinical Endocrinology And Metabolism, 85 (2000) [C1]
CitationsScopus - 62Web of Science - 56
Co-authorsBruce King, Roger Smith
1997Ni X, Chan EC, Fitter JT, Smith R, 'Nitric oxide inhibits corticotropin-releasing hormone exocytosis but not synthesis by cultured human trophoblasts', JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 82 4171-4175 (1997)
DOI10.1210/jc.82.12.4171Author URL
CitationsScopus - 32Web of Science - 26
Co-authorsRoger Smith
1997West NP, Fitter JT, Jakubzik U, Rodhe M, Guzman CA, Walker MJ, 'Non-motile mini-transposon mutants of Bordetella bronchiseptica exhibit altered abilities to invade and survive in eukaryotic cells', FEMS MICROBIOLOGY LETTERS, 146 263-269 (1997)
DOI10.1016/S0378-1097(96)00486-7Author URL
CitationsScopus - 9Web of Science - 9
1996Fitter JT, Thomas MR, Rose RJ, SteeleScott N, 'Heteroplasmy of the chloroplast genome of Medicago sativa L cv 'Regen S' confirmed by sequence analysis', THEORETICAL AND APPLIED GENETICS, 93 685-690 (1996)
DOI10.1007/BF00224063Author URL
CitationsScopus - 9Web of Science - 9
Co-authorsRay Rose
1993FITTER JT, ROSE RJ, 'INVESTIGATION OF CHLOROPLAST DNA HETEROPLASMY IN MEDICAGO-SATIVA L USING CULTURED TISSUE', THEORETICAL AND APPLIED GENETICS, 86 65-70 (1993)
Author URL
CitationsScopus - 6Web of Science - 8
Co-authorsRay Rose
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Conference (5 outputs)

YearCitationAltmetricsLink
2014Pan X, Smith R, Scott RJ, Fitter J, Zakar T, 'Corticotropin Releasing Hormone (CRH) Expression Is Controlled by DNA Methylation in the Trophoblast', REPRODUCTIVE SCIENCES (2014) [E3]
Author URL
Co-authorsRodney Scott, Roger Smith
2012Chai S, Fitter JT, Smith R, Madsen GM, 'DNA methylation of progesterone receptor isoforms in term human myometrium', Abstracts. The Annual Scientific Meeting of the Endocrine Society of Australia and the Society for Reproductive Biology 2012, Gold Coast, QLD (2012) [E3]
Co-authorsRoger Smith, Gemma Madsen
2012Pan X, Nicholson RC, Scott R, Fitter JT, Smith R, Zakar T, 'DNA methylation associated with induction of CRH gene expression in trophoblast cells', Abstracts. The Annual Scientific Meeting of the Endocrine Society of Australia and the Society for Reproductive Biology 2012, Gold Coast, QLD (2012) [E3]
Co-authorsRoger Smith, Rodney Scott
2006Minahan KL, Clulow J, Mahony MJ, Smith R, Fitter JT, 'Different environmental stresses elicit differential CRH responses in limnodynastes peronii', Endocrine Journal-Continuation of Endocrinologia Japonica, Queensland, Australia (2006) [E3]
Co-authorsJohn Clulow, Roger Smith
1990ROSE RJ, THOMAS MR, FITTER JT, 'THE TRANSFER OF CYTOPLASMIC AND NUCLEAR GENOMES BY SOMATIC HYBRIDIZATION', AUSTRALIAN JOURNAL OF PLANT PHYSIOLOGY, UNIV QUEENSLAND, BRISBANE, AUSTRALIA (1990)
Author URL
CitationsWeb of Science - 18
Co-authorsRay Rose
Show 2 more conferences
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Grants and Funding

Summary

Number of grants13
Total funding$549,054

Click on a grant title below to expand the full details for that specific grant.


20123 grants / $191,814

Nikon C2 + Si Confocal Microscope$75,000

Funding body: Ramaciotti Foundations

Funding bodyRamaciotti Foundations
Project TeamProfessor Ian Symonds, Doctor John Fitter
SchemeMajor Equipment Award
RoleInvestigator
Funding Start2012
Funding Finish2012
GNoG1200728
Type Of FundingGrant - Aust Non Government
Category3AFG
UONY

Nikon C2 + Si Confocal Microscope$75,000

Funding body: University of Newcastle

Funding bodyUniversity of Newcastle
Project TeamDoctor John Fitter, Professor Ian Symonds
SchemeEquipment Grant
RoleLead
Funding Start2012
Funding Finish2012
GNoG1200747
Type Of FundingInternal
CategoryINTE
UONY

Nikon C2 + Si Confocal Microscope $41,814

Funding body: Hunter Medical Research Institute

Funding bodyHunter Medical Research Institute
Project TeamDoctor John Fitter
SchemeEquipment Grant
RoleLead
Funding Start2012
Funding Finish2012
GNoG1201046
Type Of FundingContract - Aust Non Government
Category3AFC
UONY

20082 grants / $24,533

Equipment Grant - Bio Doc IT with M-26 (21 x 26 cm H/L transluminator)$14,933

Funding body: Hunter Medical Research Institute

Funding bodyHunter Medical Research Institute
Project TeamDoctor John Fitter
SchemeEquipment Grant
RoleLead
Funding Start2008
Funding Finish2008
GNoG0189034
Type Of FundingOther Public Sector - State
Category2OPS
UONY

LED fluorescence illuminators and filter set (525nm + 575DF20) for LAS3000 image analysis system$9,600

Funding body: NHMRC (National Health & Medical Research Council)

Funding bodyNHMRC (National Health & Medical Research Council)
Project TeamProfessor Roger Smith, Professor Ian Symonds, Conjoint Associate Professor Andrew Bisits, Conjoint Professor Tam Zakar, Doctor John Fitter, Doctor Cheng Chan, Conjoint Associate Professor Rick Nicholson, Doctor Giavanna Angeli, Doctor Kaushik Maiti, Doctor Jonathan Paul, Professor Jon Hirst, Doctor Hannah Palliser, Professor Eugenie Lumbers
SchemeEquipment Grant
RoleInvestigator
Funding Start2008
Funding Finish2008
GNoG0188543
Type Of FundingOther Public Sector - Commonwealth
Category2OPC
UONY

20071 grants / $25,670

Beta Radiation Counter Instrument facility$25,670

Funding body: NHMRC (National Health & Medical Research Council)

Funding bodyNHMRC (National Health & Medical Research Council)
Project TeamProfessor Roger Smith, Conjoint Associate Professor Vicki Clifton, Conjoint Professor Tam Zakar, Conjoint Associate Professor Rick Nicholson, Dr Mark Read, Doctor Cheng Chan, Doctor John Fitter, Conjoint Associate Professor Andrew Bisits, Professor Jon Hirst, Doctor Hannah Palliser, Professor Ian Symonds
SchemeEquipment Grant
RoleInvestigator
Funding Start2007
Funding Finish2007
GNoG0188194
Type Of FundingOther Public Sector - Commonwealth
Category2OPC
UONY

20051 grants / $7,537

Endocrine Regulation of Normal and Stress-Induced Metamorphosis in an Australian Frog: Limnodynastes peronii$7,537

Funding body: University of Newcastle

Funding bodyUniversity of Newcastle
Project TeamProfessor Michael Mahony, Doctor John Clulow, Doctor John Fitter
SchemeProject Grant
RoleInvestigator
Funding Start2005
Funding Finish2005
GNoG0184655
Type Of FundingInternal
CategoryINTE
UONY

20041 grants / $11,000

Endocrine regulation of normal and stress-induced metamorphosis in an Australian Frog: Limnodynastes peronii$11,000

Funding body: University of Newcastle

Funding bodyUniversity of Newcastle
Project TeamDoctor John Clulow, Professor Michael Mahony, Doctor John Fitter
SchemeProject Grant
RoleInvestigator
Funding Start2004
Funding Finish2004
GNoG0183520
Type Of FundingInternal
CategoryINTE
UONY

20031 grants / $12,000

Interactions of genome, phenome and environmental stress on tadpole metamorphosis$12,000

Funding body: University of Newcastle

Funding bodyUniversity of Newcastle
Project TeamProfessor Michael Mahony, Doctor John Clulow, Doctor John Fitter
SchemeProject Grant
RoleInvestigator
Funding Start2003
Funding Finish2003
GNoG0182398
Type Of FundingInternal
CategoryINTE
UONY

20021 grants / $13,000

Expression of the homeobox gene HOXA10 in human parturition$13,000

Funding body: University of Newcastle

Funding bodyUniversity of Newcastle
Project TeamDoctor John Fitter, Professor Roger Smith
SchemeProject Grant
RoleLead
Funding Start2002
Funding Finish2002
GNoG0181295
Type Of FundingInternal
CategoryINTE
UONY

20011 grants / $5,000

The role of CRH and the CRH receptor in hormonal control of frog metamorphosis$5,000

Funding body: University of Newcastle

Funding bodyUniversity of Newcastle
Project TeamDoctor John Fitter, Professor Michael Mahony, Conjoint Professor Anthony Leong
SchemeProject Grant
RoleLead
Funding Start2001
Funding Finish2001
GNoG0180088
Type Of FundingInternal
CategoryINTE
UONY

19981 grants / $14,000

The regulation and the signalling pathway of placental corticotropin-releasing hormone receptor/s in human pregnancy$14,000

Funding body: University of Newcastle

Funding bodyUniversity of Newcastle
Project TeamDoctor John Fitter, Professor Roger Smith
SchemeProject Grant
RoleLead
Funding Start1998
Funding Finish1998
GNoG0177268
Type Of FundingInternal
CategoryINTE
UONY

19961 grants / $244,500

South-East Asian Australian Preterm Labour Study Group.$244,500

Funding body: Department of Education, Training & Youth Affairs

Funding bodyDepartment of Education, Training & Youth Affairs
Project TeamEmeritus Professor William Walters, Professor Roger Smith, Conjoint Professor Warwick Giles, Conjoint Associate Professor Andrew Bisits, Doctor Cheng Chan, Dr Stephen O'Callaghan, Doctor Jenny Stewart Williams, Mrs Maria Bowman, Doctor John Fitter
SchemeTargetted Institutional Links Program
RoleInvestigator
Funding Start1996
Funding Finish1996
GNoG0176589
Type Of FundingOther Public Sector - Commonwealth
Category2OPC
UONY
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Research Supervision

Current Supervision

CommencedResearch Title / Program / Supervisor Type
2009Epigenetic Regulation of the CRH Gene in Pregnancy
General Medicine, Faculty of Health and Medicine
Co-Supervisor

Past Supervision

YearResearch Title / Program / Supervisor Type
2005The role of CRH in development and plasticity oin the amphibian Lymnodynastes peronii
Biol Sc Not Elsewhere Classifd, University of Newcastle
Co-Supervisor
2002The role of corticotropin releasing hormone and its receptor in the metamorphosis of Australian anurans
Biol Sc Not Elsewhere Classifd, University of Newcastle
Co-Supervisor
1997Regulation of Corticotropin Releasing Hormone in Human Placental Cells
Biol Sc Not Elsewhere Classifd, University of Newcastle
Sole Supervisor
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Dr John Fitter

Position

Laboratory and Research Manager
Mothers & Babies Research Centre
School of Medicine and Public Health
Faculty of Health and Medicine

Contact Details

Emailjohn.fitter@newcastle.edu.au
Phone(02) 40420361
Fax(02) 40420045

Office

RoomHMRI 3406
BuildingHunter Medical Research Institute
LocationJohn Hunter Hospital campus

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