Associate Professor Ian Grainge
Associate Professor
School of Environmental and Life Sciences (Biological Sciences)
- Email:ian.grainge@newcastle.edu.au
- Phone:4921 7238
From Oxford to Newcastle
The journey to biology research began for Dr Ian Grainge almost 20 years ago in the United Kingdom.
Two years into an undergraduate degree, Grainge's initial interest in Chemistry had begun to dwindle. He switched to genetics before obtaining his PhD in biochemistry and he hasn't looked back since.
From there he took his interest in research around the world. This included working on a natural yeast plasmid in Texas for three years before returning to work for Cancer Research UK, looking at replication proteins and protein structures (x-ray crystallography).
After doing more work on bacteria in Oxford, Grainge's idea was to link together biochemistry (how proteins work in test tubes) to the actual structural information and has carried on with this theme in his current research.
"At the moment we're trying to understand the molecular details of one of the motor proteins and the way it can bind and convert chemical energy into actual movement of DNA" explained Grainge.
"This FtsK protein is a very efficient molecular pump that moves the DNA through the cell and we're trying to understand the molecular details of how this protein machine works. It is found in most bacteria and plays an important role in the cell, co-ordinating the vital process of cell division and chromosome unlinking, which is one way bacteria keep their chromosome intact" he added.
The four year ARC Future Fellowship that Grainge was awarded in 2012 will contribute to this research, which could potentially form the basis for developing a new antibiotic to fight bacteria.
"It's amazing to get the grant both for opportunity for research and the recognition that somebody thinks my work is important. I've got more than enough work to do over the term of the Fellowship and I'll try to get students interested in the work as well to get them helping me on the project", said Grainge.
Mathematicians will also be involved with the project to try to understand the way in which the protein affects the shape of the DNA, known as DNA Topology, and how that affects the outcomes of these reactions.
Grainge's move to Newcastle 2010 was a big step to becoming independent and setting up his own lab, which was a big motivating factor in his decision making. Since his time at the University of Newcastle, he has obtained four grants, including the Fellowship, which is a major achievement.
While the future appears very busy for Grainge, he hopes to take up a few hobbies while based in Newcastle.
"I'd like to try surfing some day. I've just been too scared to try it so far. Apart from that I'm happy to stay at the University of Newcastle. I like it here," he said.
Related links
From Oxford to Newcastle
Dr Ian Grainge is interested in all aspects of how bacteria pass on their genetic information, from DNA replication to chromosome segregation and accurate
Career Summary
Biography
I am interested in all aspects of how bacteria pass on their genetic information, from DNA replication to chromosome segregation and accurate cell division. My current research focuses on two main topics. Firstly what happens when the process of DNA replication runs into a blockage and stops- how can the cell recover to restart the vital process of copying its DNA? A large number of homologous recombination proteins have been implicated in the processing of collapsed DNA replication forks and their roles, and the pathways used, will be investigated in living cells. Secondly, is the study of the FtsK protein, which co-ordinates the processes of cell division, chromosome unlinking and chromosome segregation in bacteria. Each of these processes has to be completed in a timely manner to allow the cell to divide to produce offspring with a full genetic content. The DNA translocase protein, FtsK, is a key protein in each of these processes, and could additionally act as a cell division checkpoint. Post-doctoral work: Department of Biochemistry, University of Oxford (2005-2009) Cancer Research UK (2000-2004) University of Texas at Austin (1997-2000) PhD: University of Oxford, UK (1994-1997)
Research Expertise
DNA replication in bacteria: restart of stalled replication forks The chromosome of E. coli is a circular DNA molecule which is replicated bi-directionally from a single origin (OriC). Multiple copies of the tetracycline operator (tetO) have been placed in the chromosome 16kb to one side of the origin of replication. Expression of a fluorescent tetracycline repressor (TetR-YFP) allows direct visualization of this region in a fluorescence microscope. Using this system, the origin can effectively be followed during duplication and on through the cell cycle. It was found that overexpression of tetR led to cell inviability. The viability of the cells could be recovered by addition of the effector molecule, anhydrous tetracycline (AT) which reduces the binding of TetR to tetO. Analysis of replication in cells overexpressing TetR showed that the array formed an effective block to replication forks. 2-D gel analysis shows that replication forks can proceed fewer than 500 bp into the tetO array before stalling occurs. Further it is seen that addition of AT leads to a very rapid restart of these stalled forks. Restart of the forks was examined in recA and recB mutant strains and it was found that restart occurred with very similar kinetics to those seen in a wild-type background. This has led us to propose that a stalled replication fork is stable in E. coli for a period of at least 2 hours and that restart does not require recombination. Current work is focusing on what happens when the replisome, stalled at the tetO array block, is disassembled, with a view to understanding replication fork restart pathways and kinetics in vivo. Using this system replication restart can be followed in various mutant backgrounds by examination of fluorescent repressor operators, and by using 2-D gels, to effectively dissect in vivo restart pathways. FtsK: a fast molecular motor The multifunctional FtsK protein is involved in cell division and DNA segregation in E. coli. The C-terminal portion of this large protein forms a hexameric ring with the ability to translocate DNA at speeds of ~ 5kb/sec. The FtsK protein is loaded on DNA in a specific orientation by interactions with polarized sequences on the chromosome which ensure that the protein will subsequently move towards the dif site located in the terminus of the chromosome. Once there, FtsK also interacts with the site-specific recombinase XerD to promote recombination between two dif sites. Further, as a result of translocation the two recombining dif sites are brought together in a topologically simple manner so that recombination leads to a simplification of topology, and eventually chromosome unlinking. Using a variety of biochemical techniques the mechanism of directed loading upon DNA, DNA translocation and activation of recombination within a specific synapse topology is being investigated. Using covalently linked multimers of the translocase protion of the protein, hexameric rings can be formed within which mutations can be targeted to specific subunits. This allows more defined analysis of the mechanism of loading and translocation than would otherwise be possible.
Teaching Expertise
Present course taught: BIOL2010- Biochemistry CHEM3550 Medicinal Chemistry BIOL3100 Microbiology BIOL2230 Biomolecules Courses lectured at the University of Oxford, Department of Biochemistry: Modern Molecular Biology: Methods DNA: Replication and Recombination
Administrative Expertise
Member of Institutional Biosafety Committee Member of Faculty Research and Research Training Committee Convenor of Biological Sciences seminar series.
Qualifications
- PhD, University of Oxford - UK
- Bachelor of Arts, University of Cambridge - UK
- Master of Arts, University of Cambridge - UK
Keywords
- BIOL2010
- BIOL2230
- BIOL3100
- CHEM3550
- Cell division
- Chromosome segregation
- DNA translocation
- Microbiology
- Novel antibiotics
- Site-specific recombination
- antibiotic resistance
- bacteriophage
Fields of Research
Code | Description | Percentage |
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310704 | Microbial genetics | 60 |
310106 | Enzymes | 20 |
310706 | Virology | 20 |
Professional Experience
UON Appointment
Title | Organisation / Department |
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Associate Professor | University of Newcastle School of Environmental and Life Sciences Australia |
Academic appointment
Dates | Title | Organisation / Department |
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1/11/2012 - |
Fellow ARC ARC - Discovery - Future Fellowships |
University of Newcastle Australia |
Membership
Dates | Title | Organisation / Department |
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1/1/2012 - | Membership - Australian Society for Microbiology | Australian Society for Microbiology Australia |
Teaching
Code | Course | Role | Duration |
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BIOL2010 |
Biochemistry Faculty of Science | University of Newcastle |
Lecturer | 15/2/2010 - 5/8/2021 |
Publications
For publications that are currently unpublished or in-press, details are shown in italics.
Book (2 outputs)
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2017 |
Biomotors, CRC Press
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2007 |
Mobile DNA II, Wiley (2007)
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Chapter (7 outputs)
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2023 |
Guo P, Noji H, Yengo CM, Zhao Z, Grainge I, 'Biological Nanomotors with Linear, Rotation, or Revolution Motion Mechanism', Biomotors and their Nanobiotechnology Applications 1-42 (2023) The ubiquitous biological nanomotors were once classified into two categories: linear and rotation motors. In 2013, a third type of biomotor¿revolution without rotation (see anima... [more] The ubiquitous biological nanomotors were once classified into two categories: linear and rotation motors. In 2013, a third type of biomotor¿revolution without rotation (see animations: https://nanobio.uky.edu/movie.html" xmlns:xlink="https://www.w3.org/1999/xlink">https://nanobio.uky.edu/movie.html)¿was discovered and found to be widespread among bacteria, eukaryotic viruses, and dsDNA bacteriophages. This review focuses on recent findings of various aspects of motors, including chirality, stoichiometry, channel size, entropy, conformational change, and energy usage rate in a variety of well-studied motors, including FOF1 ATPase, helicases, viral dsDNA-packaging motors, bacterial chromosome translocases, myosin, kinesin, and dynein. In particular, dsDNA translocases are used to illustrate how these features relate to the motion mechanism, and how nature elegantly evolved a revolution mechanism to avoid coiling and tangling during lengthy dsDNA genome transportation in cell division. Motor chirality and channel size are the two factors to distinguish rotation motors from revolution motors. Rotation motors use the right-handed channels to drive the right-handed dsDNA, similar to the way a nut drives the bolt with threads in the same orientation; revolution motors use the left-handed motor channels to revolve the right-handed dsDNA. Rotation motors use small channels (< 2 nm in diameter) for the close contact of the channel wall with the 2-nm dsDNA bolt; revolution motors use larger channels (> 3 nm in diameter) with room for the bolt to revolve. Binding and hydrolysis of ATP are linked to different conformational entropy changes in the motor that lead to altered affinity for the substrate and allow work to be done, for example, unwind DNA in a helicase, or move DNA directionally for a translocase.
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2020 |
Mettrick KA, Weaver GM, Grainge I, 'Neutral Neutral 2-Dimensional Agarose Gel Electrophoresis for Visualization of E. coli DNA Replication Structures', Methods in Molecular Biology, Springer Nature, New York, NY 61-72 (2020)
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2012 |
Doherty GP, Mettrick KA, Grainge IR, Lewis PJ, 'Imaging fluorescent protein fusions in live bacteria', Methods in Microbiology, Academic Press, Kidlington, Ox 107-126 (2012) [B1]
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2005 |
Voziyanov Y, Grainge I, Jayaram M, 'Applications of fungal site-specific recombination as a tool in biotechnology and basic biology', Applied Mycology and Biotechnology 189-210 (2005) [B1] Two classes of conservative site-specific recombinases, those belonging to the tyrosine and serine families, have been identified, and several of its members characterized in gene... [more] Two classes of conservative site-specific recombinases, those belonging to the tyrosine and serine families, have been identified, and several of its members characterized in genetic and biochemical detail. These families are named after the active site amino acid, tyrosine or serine, that is utilized as the nucleophile during the strand breaking step of recombination. The Flp recombinase encoded by the 2 micron plasmid of Saccharomyces cerevisiae and related recombinases encoded by similar plasmids found in other yeast species belong to the tyrosine family. The Flp protein has provided several insights into the mechanism of target DNA recognition, strand cleavage and strand exchange during the recombination reaction. Here we describe how the Flp system has been used as a tool for tackling basic and applied problems in biology. © 2005 Elsevier B.V. All rights reserved.
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Journal article (49 outputs)
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2023 |
Basta T, Crozat E, Grainge I, 'Editorial: Chromosome architecture and DNA topology in prokaryotes.', Front Microbiol, 14 1355036 (2023)
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2022 |
Chan H, Mohamed AMT, Grainge I, Rodrigues CDA, 'FtsK and SpoIIIE, coordinators of chromosome segregation and envelope remodeling in bacteria', Trends in Microbiology, 30 480-494 (2022) [C1] The translocation of DNA during bacterial cytokinesis is mediated by the SpoIIIE/FtsK family of proteins. These proteins ensure efficient chromosome segregation into sister cells ... [more] The translocation of DNA during bacterial cytokinesis is mediated by the SpoIIIE/FtsK family of proteins. These proteins ensure efficient chromosome segregation into sister cells by ATP-driven translocation of DNA and they control chromosome dimer resolution. How FtsK/SpoIIIE mediate chromosome translocation during cytokinesis in Gram-positive and Gram-negative organisms has been the subject of debate. Studies on FtsK in Escherichia coli, and recent work on SpoIIIE in Bacillus subtilis, have identified interactions between each translocase and the division machinery, supporting the idea that SpoIIIE and FtsK coordinate the final steps of cytokinesis with completion of chromosome segregation. Here we summarize and discuss the view that SpoIIIE and FtsK play similar roles in coordinating cytokinesis with chromosome segregation, during growth and differentiation.
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2022 |
Bolan S, Seshadri B, Kunhikrishnan A, Grainge I, Talley NJ, Bolan N, Naidu R, 'Differential toxicity of potentially toxic elements to human gut microbes', CHEMOSPHERE, 303 (2022) [C1]
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2021 |
Bhagwat G, Tran TKA, Lamb D, Senathirajah K, Grainge I, O Connor W, et al., 'Biofilms Enhance the Adsorption of Toxic Contaminants on Plastic Microfibers under Environmentally Relevant Conditions', Environmental Science and Technology, 55 8877-8887 (2021) [C1] Microplastics (MPs) exposed to the natural environment provide an ideal surface for biofilm formation, which potentially acts as a reactive phase facilitating the sorption of haza... [more] Microplastics (MPs) exposed to the natural environment provide an ideal surface for biofilm formation, which potentially acts as a reactive phase facilitating the sorption of hazardous contaminants. Until now, changes in the contaminant sorption capacity of MPs due to biofilm formation have not been quantified. This is the first study that compared the capacity of naturally aged, biofilm-covered microplastic fibers (BMFs) to adsorb perfluorooctane sulfonate (PFOS) and lead (Pb) at environmentally relevant concentrations. Changes in the surface properties and morphology of aged microplastic fibers (MF) were studied by surface area analysis, infrared spectroscopy, and scanning electron microscopy. Results revealed that aged MFs exhibited higher surface areas because of biomass accumulation compared to virgin samples and followed the order polypropylene>polyethylene>nylon>polyester. The concentrations of adsorbed Pb and PFOS were 4-25% and 20-85% higher in aged MFs and varied among the polymer types. The increased contaminant adsorption was linked with the altered surface area and the hydrophobic/hydrophilic characteristics of the samples. Overall, the present study demonstrates that biofilms play a decisive role in contaminant-plastic interactions and significantly enhance the vector potential of MFs for toxic environmental contaminants. We anticipate that knowledge generated from this study will help refine the planetary risk assessment of MPs.
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2021 |
Bhagwat G, Carbery M, Anh Tran TK, Grainge I, O'Connor W, Palanisami T, 'Fingerprinting Plastic-Associated Inorganic and Organic Matter on Plastic Aged in the Marine Environment for a Decade', Environmental Science and Technology, 55 7407-7417 (2021) [C1] The long-term aging of plastic leads to weathering and biofouling that can influence the behavior and fate of plastic in the marine environment. This is the first study to fingerp... [more] The long-term aging of plastic leads to weathering and biofouling that can influence the behavior and fate of plastic in the marine environment. This is the first study to fingerprint the contaminant profiles and bacterial communities present in plastic-associated inorganic and organic matter (PIOM) isolated from 10 year-aged plastic. Plastic sleeves were sampled from an oyster aquaculture farm and the PIOM was isolated from the intertidal, subtidal, and sediment-buried segments to investigate the levels of metal(loid)s, polyaromatic hydrocarbons (PAHs), per-fluoroalkyl substances (PFAS) and explore the microbial community composition. Results indicated that the PIOM present on long-term aged high-density polyethylene plastic harbored high concentrations of metal(loid)s, PAHs, and PFAS. Metagenomic analysis revealed that the bacterial composition in the PIOM differed by habitat type, which consisted of potentially pathogenic taxa including Vibrio, Shewanella, and Psychrobacter. This study provides new insights into PIOM as a potential sink for hazardous environmental contaminants and its role in enhancing the vector potential of plastic. Therefore, we recommend the inclusion of PIOM analysis in current biomonitoring regimes and that plastics be used with caution in aquaculture settings to safeguard valuable food resources, particularly in areas of point-source contamination.
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2021 |
Bhagwat G, O Connor W, Grainge I, Palanisami T, 'Understanding the Fundamental Basis for Biofilm Formation on Plastic Surfaces: Role of Conditioning Films', Frontiers in Microbiology, 12 (2021) [C1] Conditioning films (CFs) are surface coatings formed by the adsorption of biomolecules from the surrounding environment that can modify the material-specific surface properties an... [more] Conditioning films (CFs) are surface coatings formed by the adsorption of biomolecules from the surrounding environment that can modify the material-specific surface properties and precedes the attachment of microorganisms. Hence, CFs are a biologically relevant identity that could govern the behavior and fate of microplastics in the aquatic environment. In the present study, polyethylene terephthalate (PET) and polylactic acid (PLA) plastic cards were immersed in natural seawater to allow the formation of CFs. The changes in the surface roughness after 24 h were investigated by atomic force microscopy (AFM), and the surface changes were visualized by scanning electron microscopy (SEM). The global elemental composition of the conditioned surface was investigated by energy dispersive spectroscopy (EDS). Results indicated that marine conditioning of PET and PLA samples for 24 h resulted in an increase of ~11 and 31% in the average surface roughness, respectively. SEM images revealed the attachment of coccoid-shaped bacterial cells on the conditioned surfaces, and the accumulation of salts of sodium and phosphate-containing precipitates was revealed through the EDS analysis. The results indicate that the increase in surface roughness due to conditioning is linked to a material¿s hydrophilicity leading to a rapid attachment of bacteria on the surfaces. Further investigations into the CFs can unfold crucial knowledge surrounding the plastic-microbe interaction that has implications for medical, industrial, and environmental research.
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2021 |
Bolan S, Seshadri B, Grainge I, Talley NJ, Naidu R, 'Gut microbes modulate bioaccessibility of lead in soil', Chemosphere, 270 (2021) [C1] Metabolic uptake of lead (Pb) is controlled by its bioaccessibility. Most studies have examined bioaccessibility of Pb in the absence of gut microbes, which play an important role... [more] Metabolic uptake of lead (Pb) is controlled by its bioaccessibility. Most studies have examined bioaccessibility of Pb in the absence of gut microbes, which play an important role in the metabolic uptake of nutrients and metal(loid)s in intestine. In this study, we examined the effect of three gut microbes, from various locations in the gut, on the bioaccessibility of soil ingested Pb. The gut microbes include Lactobacillus acidophilus, Lactobacillus rhamnosus and Escherichia coli. Lead toxicity to these three microbes was also examined at various pH values. Bioaccessibility of Pb was measured using gastric and intestinal extractions. Both Pb spiked and Pb-contaminated shooting range field soils were used to measure Pb bioaccessibility in the presence and absence of gut microbes. The results indicated that Pb toxicity to gut microbes, as measured by LD50 value, decreased with increasing pH, and was higher for Lactobacillus species. Gut microbes decreased the bioaccessible Pb; the effect was more pronounced at low pH, mimicking gastric conditions than in conditions closer to the intestine. Lead adsorption by these microbes increased at the higher pH tested, and E. coli adsorbed higher amounts of Pb than did the Lactobacillus species. The effect of gut microbes on reducing Pb bioaccessibility may be attributed to microbially-induced immobilization of Pb through adsorption, precipitation, and complexation reactions. The study demonstrates that bioaccessibility and subsequently bioavailability of metal(loid)s can be modulated by gut microbes, and it is important to undertake bioaccessibility measurements in the presence of gut microbes.
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2021 |
Bolan S, Seshadri B, Keely S, Kunhikrishnan A, Bruce J, Grainge I, et al., 'Bioavailability of arsenic, cadmium, lead and mercury as measured by intestinal permeability', Scientific Reports, 11 (2021) [C1] In this study, the intestinal permeability of metal(loid)s (MLs) such as arsenic (As), cadmium (Cd), lead (Pb) and mercury (Hg) was examined, as influenced by gut microbes and che... [more] In this study, the intestinal permeability of metal(loid)s (MLs) such as arsenic (As), cadmium (Cd), lead (Pb) and mercury (Hg) was examined, as influenced by gut microbes and chelating agents using an in vitro gastrointestinal/Caco-2 cell intestinal epithelium model. The results showed that in the presence of gut microbes or chelating agents, there was a significant decrease in the permeability of MLs (As-7.5%, Cd-6.3%, Pb-7.9% and Hg-8.2%) as measured by apparent permeability coefficient value (Papp), with differences in ML retention and complexation amongst the chelants and the gut microbes. The decrease in ML permeability varied amongst the MLs. Chelating agents reduce intestinal absorption of MLs by forming complexes thereby making them less permeable. In the case of gut bacteria, the decrease in the intestinal permeability of MLs may be associated to a direct protection of the intestinal barrier against the MLs or indirect intestinal ML sequestration by the gut bacteria through adsorption on bacterial surface. Thus, both gut microbes and chelating agents can be used to decrease the intestinal permeability of MLs, thereby mitigating their toxicity.
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2021 |
Bhagwat G, Zhu Q, O'Connor W, Subashchandrabose S, Grainge I, Knight R, Palanisami T, 'Exploring the Composition and Functions of Plastic Microbiome Using Whole-Genome Sequencing', Environmental Science and Technology, 55 4899-4913 (2021) [C1] Besides the ecotoxicological consequences of microplastics and associated chemicals, the association of microbes on plastics has greater environmental implications as microplastic... [more] Besides the ecotoxicological consequences of microplastics and associated chemicals, the association of microbes on plastics has greater environmental implications as microplastics may select for unique microbiome participating in environmentally significant functions. Despite this, the functional potential of the microbiome associated with different types of plastics is understudied. Here, we investigate the interaction between plastic and marine biofilm-forming microorganisms through a whole-genome sequencing approach on four types of microplastics incubated in the marine environment. Taxonomic analysis suggested that the microplastic surfaces exhibit unique microbial profiles and niche partitioning among the substrates. In particular, the abundance of Vibrio alginolyticus and Vibrio campbellii suggested that microplastic pollution may pose a potential risk to the marine food chain and negatively impact aquaculture industries. Microbial genera involved in xenobiotic compound degradation, carbon cycling, and genes associated with the type IV secretion system, conjugal transfer protein TraG, plant-pathogen interaction, CusA/CzcA family heavy metal efflux transfer proteins, and TolC family proteins were significantly enriched on all the substrates, indicating the variety of processes operated by the plastic-microbiome. The present study gives a detailed characterization of the rapidly altering microbial composition and gene pools on plastics and adds new knowledge surrounding the environmental ramifications of marine plastic pollution.
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2020 |
Balalovski P, Grainge I, 'Mobilization of pdif modules in Acinetobacter: A novel mechanism for antibiotic resistance gene shuffling?', Molecular Microbiology, 114 699-709 (2020) [C1] XerCD-dif site-specific recombination is a well characterized system, found in most bacteria and archaea. Its role is resolution of chromosomal dimers that arise from homologous r... [more] XerCD-dif site-specific recombination is a well characterized system, found in most bacteria and archaea. Its role is resolution of chromosomal dimers that arise from homologous recombination. Xer-mediated recombination is also used by several plasmids for multimer resolution to enhance stability and by some phage for integration into the chromosome. In the past decade, it has been hypothesized that an alternate and novel function exists for this system in the dissemination of genetic elements, notably antibiotic resistance genes, in Acinetobacter species. Currently the mechanism underlying this apparent genetic mobility is unknown. Multidrug resistant Acinetobacter baumannii is an increasingly problematic pathogen that can cause recurring infections. Sequencing of numerous plasmids from clinical isolates of A. baumannii revealed the presence of possible mobile modules: genes were found flanked by pairs of Xer recombination sites, called plasmid-dif (pdif) sites. These modules have been identified in multiple otherwise unrelated plasmids and in different genetic contexts suggesting they are mobile elements. In most cases, the pairs of sites flanking a gene (or genes) are in inverted repeat, but there can be multiple modules per plasmid providing pairs of recombination sites that can be used for inversion or fusion/deletion reactions; as many as 16 pdif sites have been seen in a single plasmid. Similar modules including genes for surviving environmental toxins have also been found in strains of Acinetobacter Iwoffi isolated from permafrost cores; this suggests that these mobile modules are an ancient adaptation and not a novel response to antibiotic pressure. These modules bear all the hallmarks of mobile genetic elements, yet, their movement has never been directly observed to date. This review gives an overview of the current state of this novel research field.
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2019 |
Conte E, Mende L, Grainge I, Colloms SD, 'A Mini-ISY100 Transposon Delivery System Effective in gamma Proteobacteria', FRONTIERS IN MICROBIOLOGY, 10 (2019) [C1]
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2019 |
Weaver GM, Mettrick KA, Corocher T-A, Graham A, Grainge I, 'Replication fork collapse at a protein-DNA roadblock leads to fork reversal, promoted by the RecQ helicase', MOLECULAR MICROBIOLOGY, 111 455-472 (2019) [C1]
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2016 |
Keller AN, Xin Y, Boer S, Reinhardt J, Baker R, Arciszewska LK, et al., 'Activation of Xer-recombination at
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2016 |
Mettrick KA, Lawrence N, Mason C, Weaver GM, Corocher TA, Grainge I, 'Inducing a site specific replication blockage in E. coli using a fluorescent repressor operator system', Journal of Visualized Experiments, 2016 (2016) [C1] Obstacles present on DNA, including tightly-bound proteins and various lesions, can severely inhibit the progression of the cell¿s replication machinery. The stalling of a repliso... [more] Obstacles present on DNA, including tightly-bound proteins and various lesions, can severely inhibit the progression of the cell¿s replication machinery. The stalling of a replisome can lead to its dissociation from the chromosome, either in part or its entirety, leading to the collapse of the replication fork. The recovery from this collapse is a necessity for the cell to accurately complete chromosomal duplication and subsequently divide. Therefore, when the collapse occurs, the cell has evolved diverse mechanisms that take place to restore the DNA fork and allow replication to be completed with high fidelity. Previously, these replication repair pathways in bacteria have been studied using UV damage, which has the disadvantage of not being localized to a known site. This manuscript describes a system utilizing a Fluorescence Repressor Operator System (FROS) to create a site-specific protein block that can induce the stalling and collapse of replication forks in Escherichia coli. Protocols detail how the status of replication can be visualized in single living cells using fluorescence microscopy and DNA replication intermediates can be analyzed by 2-dimensional agarose gel electrophoresis. Temperature sensitive mutants of replisome components (e.g. DnaBts) can be incorporated into the system to induce a synchronous collapse of the replication forks. Furthermore, the roles of the recombination proteins and helicases that are involved in these processes can be studied using genetic knockouts within this system.
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2016 |
Mettrick KA, Grainge I, 'Stability of blocked replication forks in vivo', Nucleic Acids Research, 44 657-668 (2016) [C1]
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2016 |
Guo P, Noji H, Yengo CM, Zhao Z, Grainge I, 'Biological Nanomotors with a Revolution, Linear, or Rotation Motion Mechanism', MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS, 80 161-186 (2016) [C1]
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2014 |
Guo P, Grainge I, Zhao Z, Vieweger M, 'Two classes of nucleic acid translocation motors: Rotation and revolution without rotation', Cell and Bioscience, 4 (2014) [C1] Biomotors are extensively involved in biological processes including cell mitosis, bacterial binary fission, DNA replication, DNA repair, homologous recombination, Holliday juncti... [more] Biomotors are extensively involved in biological processes including cell mitosis, bacterial binary fission, DNA replication, DNA repair, homologous recombination, Holliday junction resolution, RNA transcription, and viral genome packaging. Traditionally, they were classified into two categories including linear and rotation motors. In 2013, a third class of motor by revolution mechanism without rotation was discovered. In this issue of " Structure and mechanisms of nanomotors in the cells" , four comprehensive reviews are published to address the latest advancements of the structure and motion mechanism of a variety of biomotors in archaea, animal viruses, bacteria, and bacteriophages.
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2013 |
Shimokawa K, Ishihara K, Grainge I, Sherratt DJ, Vazquez M, 'FtsK-dependent XerCD-dif recombination unlinks replication catenanes in a stepwise manner.', Proc Natl Acad Sci U S A, 110 20906-20911 (2013) [C1]
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2013 |
Grainge I, 'Simple topology: FtsK-directed recombination at the dif site', BIOCHEMICAL SOCIETY TRANSACTIONS, 41 595-600 (2013) [C1]
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2011 |
Grainge IR, Lesterlin C, Sherratt DJ, 'Activation of XerCD-dif recombination by the FtsK DNA translocase', Nucleic Acids Research, 39 5140-5148 (2011) [C1]
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2010 |
Grainge IR, 'FtsK - a bacterial cell division checkpoint?', Molecular Microbiology, 78 1055-1057 (2010) [C1]
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2010 |
Crozat E, Grainge IR, 'FtsK DNA translocase: The fast motor that knows where it's going', ChemBioChem, 11 2232-2243 (2010) [C1]
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2007 |
Grainge I, Sherratt DJ, 'Site-specific recombination', Topics in Current Genetics, 17 27-52 (2007) Site-specific recombination is a reaction in which a pair of genetically defined sites undergoes reciprocal exchange ("crossing-over") via a recombinase-mediated DNA bre... [more] Site-specific recombination is a reaction in which a pair of genetically defined sites undergoes reciprocal exchange ("crossing-over") via a recombinase-mediated DNA breakage and joining process. Such reactions have a wide range of biological outcomes, from integration and excision of virus genomes into and out of host chromosomes, to acquisition of novel genes and drug resistance, and even facilitating bacterial chromosome segregation. Two distinct families of recombinases exist, designated by their active site residues. In both these families recombination is carried out by a core of four recombinase monomers acting at two synapsed DNA sites. In many cases additional recombinase monomers and/or accessory proteins act at adjacent DNA sites to facilitate synapsis and often play a critical role in determining reaction topology. Here, the mechanism of site-specific recombination reactions is examined for both site-specific recombinase families, as well as for related proteins that mediate variant reactions, such as integrons and the integrases of conjugative transposons. © 2006 Springer-Verlag Berlin Heidelberg.
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2007 |
Grainge I, Bregu M, Vazquez M, Sivanathan V, Ip SCY, Sherratt DJ, 'Unlinking chromosome catenanes in vivo by site-specific recombination', EMBO JOURNAL, 26 4228-4238 (2007) [C1]
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2006 |
Grainge I, Gaudier M, Schuwirth BS, Westcott SL, Sandall J, Atanassova N, Wigley DB, 'Biochemical analysis of a DNA replication origin in the archaeon Aeropyrum pernix', JOURNAL OF MOLECULAR BIOLOGY, 363 355-369 (2006) [C1]
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2006 |
Possoz C, Filipe SR, Grainge I, Sherratt DJ, 'Tracking of controlled Escherichia coli replication fork stalling and restart at repressor-bound DNA in vivo', EMBO JOURNAL, 25 2596-2604 (2006) [C1]
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2004 |
Singleton MR, Morales R, Grainge I, Cook N, Isupov MN, Wigley DB, 'Conformational changes induced by nucleotide binding in Cdc6/ORC from Aeropyrum pernix', JOURNAL OF MOLECULAR BIOLOGY, 343 547-557 (2004) [C1]
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2003 |
Grainge I, Scaife S, Wigley DB, 'Biochemical analysis of components of the pre-replication complex of Archaeoglobus fulgidus', NUCLEIC ACIDS RESEARCH, 31 4888-4898 (2003) [C1]
|
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2002 | Sau AK, Tribble GD, Grainge I, Frohlich RF, Knudsen BR, Jayaram M, 'Biochemical and kinetic analysis of the RNase active sites of the integrase/tyrosine family site-specific recombinases. (vol 276, pg 46612, 2001)', JOURNAL OF BIOLOGICAL CHEMISTRY, 277 6758-6758 (2002) | ||||||||||
2002 | Sau AK, DeVue Tribble G, Grainge I, Frøhlich RF, Knudsen BR, Jayaram M, 'Erratum: Biochemical and kinetic analysis of the RNase active sites of the integrase/tyrosine family site-specific recombinases (Journal of Biological Chemistry (2001) 276 (46612-46623))', Journal of Biological Chemistry, 277 6758 (2002) | ||||||||||
2002 |
Grainge I, Pathania S, Vologodskii A, Harshey RM, Jayaram M, 'Symmetric DNA sites are functionally asymmetric within Flp and Cre site-specific DNA recombination synapses', JOURNAL OF MOLECULAR BIOLOGY, 320 515-527 (2002) [C1]
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2001 |
Frohlich RF, Hansen SG, Lisby M, Grainge I, Westergaard O, Jayaram M, Knudsen BR, 'Inhibition of Flp recombinase by the topoisomerase I-targeting drugs, camptothecin and NSC-314622', JOURNAL OF BIOLOGICAL CHEMISTRY, 276 6993-6997 (2001) [C1]
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2001 |
San AK, Tribble GD, Grainge I, Frohlich RF, Knudsen BR, Jayaram M, 'Biochemical and kinetic analysis of the RNase active sites of the integrase/tyrosine family site-specific DNA recombinases', JOURNAL OF BIOLOGICAL CHEMISTRY, 276 46612-46623 (2001)
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2001 |
Grainge I, Lee J, Xu CJ, Jayaram M, 'DNA recombination and RNA cleavage activities of the Flp protein: Roles of two histidine residues in the orientation and activation of the nucleophile for strand cleavage', JOURNAL OF MOLECULAR BIOLOGY, 314 717-733 (2001) [C1]
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2000 |
Grainge I, Buck D, Jayaram M, 'Geometry of site alignment during int family recombination: Antiparallel synapsis by the Flp recombinase', JOURNAL OF MOLECULAR BIOLOGY, 298 749-764 (2000) [C1]
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1999 |
Grainge I, Sherratt DJ, 'Xer site-specific recombination - DNA strand rejoining by recombinase XerC', JOURNAL OF BIOLOGICAL CHEMISTRY, 274 6763-6769 (1999) [C1]
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1999 |
Lee J, Jayaram M, Grainge I, 'Wild-type Flp recombinase cleaves DNA in trans', EMBO JOURNAL, 18 784-791 (1999) [C1]
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1998 |
Xu CJ, Grainge I, Lee J, Harshey RM, Jayaram M, 'Unveiling two distinct ribonuclease activities and a topoisomerase activity in a site-specific DNA recombinase', MOLECULAR CELL, 1 729-739 (1998) [C1]
|
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Show 46 more journal articles |
Review (1 outputs)
Year | Citation | Altmetrics | Link | |||||
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1999 |
Grainge I, Jayaram M, 'The integrase family of recombinases: organization and function of the active site', MOLECULAR MICROBIOLOGY (1999) [D1]
|
Conference (1 outputs)
Year | Citation | Altmetrics | Link | ||
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2008 |
Lowe J, Massey TH, Mercogliano CP, Allen MD, Grainge I, Sherratt DJ, 'DNA translocation by hexameric FtsK', ACTA CRYSTALLOGRAPHICA A-FOUNDATION AND ADVANCES (2008)
|
Preprint (1 outputs)
Year | Citation | Altmetrics | Link | ||
---|---|---|---|---|---|
2018 |
Weaver GM, Mettrick KA, Corocher T-A, Graham A, Grainge I, 'Replication fork collapse at a protein-DNA roadblock leads to fork reversal, promoted by the RecQ helicase (2018)
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Grants and Funding
Summary
Number of grants | 23 |
---|---|
Total funding | $11,299,746 |
Click on a grant title below to expand the full details for that specific grant.
20224 grants / $1,819,642
Biomass optimisation and microbial biopolymer synthesis for the compostable bioplastic production$1,309,295
Funding body: Department of Industry, Science, Energy and Resources
Funding body | Department of Industry, Science, Energy and Resources |
---|---|
Project Team | Doctor Thava Palanisami, Associate Professor Ian Grainge, Associate Professor Ajay Karakoti, Miss Haryni Jayaradhika Raghuraman Rengarajan, Professor Ajayan Vinu, Professor Jiabao Yi |
Scheme | Regional Decentralisation Agenda - Securing Raw Materials Program |
Role | Investigator |
Funding Start | 2022 |
Funding Finish | 2023 |
GNo | G2101319 |
Type Of Funding | C2200 - Aust Commonwealth – Other |
Category | 2200 |
UON | Y |
BioSHeM: A High-Resolution Imaging and Spectroscopic Helium Atom Microscope$420,347
Funding body: ARC (Australian Research Council)
Funding body | ARC (Australian Research Council) |
---|---|
Project Team | Professor Paul Dastoor, Laureate Professor Roger Smith, Professor Alan Brichta, Professor Chris Dayas, Associate Professor Ian Grainge, Jamie Quinton, Elizabeth Dinsdale, Prof Peter Cumpson, Jane Evans, Elizabeth Dinsdale, Jane Evans, Jamie Quinton |
Scheme | Linkage Infrastructure Equipment & Facilities (LIEF) |
Role | Investigator |
Funding Start | 2022 |
Funding Finish | 2022 |
GNo | G2100336 |
Type Of Funding | Scheme excluded from IGS |
Category | EXCL |
UON | Y |
BioSHeM: A High-Resolution Imaging and Spectroscopic Helium Atom Microscope$50,000
Funding body: Flinders University
Funding body | Flinders University |
---|---|
Project Team | Professor Paul Dastoor, Laureate Professor Roger Smith, Professor Alan Brichta, Professor Chris Dayas, Associate Professor Ian Grainge, Jamie Quinton, Elizabeth Dinsdale, Prof Peter Cumpson, Jane Evans, Elizabeth Dinsdale, Jane Evans, Jamie Quinton |
Scheme | Linkage Infrastructure Equipment & Facilities (LIEF) Partner Funding |
Role | Investigator |
Funding Start | 2022 |
Funding Finish | 2022 |
GNo | G2201230 |
Type Of Funding | Scheme excluded from IGS |
Category | EXCL |
UON | Y |
BioSHeM: A High-Resolution Imaging and Spectroscopic Helium Atom Microscope$40,000
Funding body: University of New South Wales
Funding body | University of New South Wales |
---|---|
Project Team | Professor Paul Dastoor, Laureate Professor Roger Smith, Professor Alan Brichta, Professor Chris Dayas, Associate Professor Ian Grainge, Jamie Quinton, Elizabeth Dinsdale, Prof Peter Cumpson, Jane Evans, Elizabeth Dinsdale, Jane Evans, Jamie Quinton |
Scheme | Linkage Infrastructure Equipment & Facilities (LIEF) Partner Funding |
Role | Investigator |
Funding Start | 2022 |
Funding Finish | 2022 |
GNo | G2201231 |
Type Of Funding | Scheme excluded from IGS |
Category | EXCL |
UON | Y |
20211 grants / $2,119,901
Novel Bioplastic Products from Biomass: Development, testing and validation$2,119,901
Funding body: Innovationclub Pty Ltd
Funding body | Innovationclub Pty Ltd |
---|---|
Project Team | Doctor Thava Palanisami, Professor Ajayan Vinu, Associate Professor Ajay Karakoti, Professor Jiabao Yi, Associate Professor Ian Grainge, . Shiv Basant Kumar, Dr Augusti Mary Priyanka Joseph Stalin, Dr Augusti Mary Priyanka Joseph Stalin |
Scheme | Research Grant |
Role | Investigator |
Funding Start | 2021 |
Funding Finish | 2023 |
GNo | G2001477 |
Type Of Funding | C3100 – Aust For Profit |
Category | 3100 |
UON | Y |
20192 grants / $4,881,745
Yeast fermentation and strain optimisation for the Ethtec Pilot Plant Project$4,857,314
Funding body: Ethanol Technologies Limited
Funding body | Ethanol Technologies Limited |
---|---|
Project Team | Associate Professor Ian Grainge, Professor Richard Bush, Dr Geoff Doherty, Cprof PETER Lewis |
Scheme | Research Grant |
Role | Lead |
Funding Start | 2019 |
Funding Finish | 2027 |
GNo | G1900188 |
Type Of Funding | C3100 – Aust For Profit |
Category | 3100 |
UON | Y |
Establishment of an Antimicrobial Research Taskforce in NSW: NUW ART$24,431
Funding body: NUW Alliance
Funding body | NUW Alliance |
---|---|
Project Team | Cprof PETER Lewis, Associate Professor Ian Grainge, Associate Professor Karl Hassan, Professor Brett Neilan, Doctor Jennette Sakoff, Associate Professor Naresh Kumar, Professor Mike Manefield, Professor Mark Willcox, Professor Antoine van Oijen, Professor Nicholas Dixon, Associate Professor Aaron Oakley, Dr Michael Kelso |
Scheme | 2019 Projects |
Role | Investigator |
Funding Start | 2019 |
Funding Finish | 2019 |
GNo | G1801287 |
Type Of Funding | C3100 – Aust For Profit |
Category | 3100 |
UON | Y |
20181 grants / $20,000
Analysis of luminal bacteria at the site of colorectal anastomoses and their association with anastomotic leaks$20,000
Funding body: Hunter Medical Research Institute
Funding body | Hunter Medical Research Institute |
---|---|
Project Team | Professor Simon Keely, Doctor Peter Pockney, Doctor Steve Smith, Associate Professor Ian Grainge, Doctor Andrea Johns |
Scheme | Project Grant |
Role | Investigator |
Funding Start | 2018 |
Funding Finish | 2018 |
GNo | G1701630 |
Type Of Funding | C3300 – Aust Philanthropy |
Category | 3300 |
UON | Y |
20171 grants / $50,000
UON 2017 Researcher Equipment Grant $50,000
Funding body: University of Newcastle
Funding body | University of Newcastle |
---|---|
Project Team | Associate Professor Ian Grainge |
Scheme | Researcher Equipment Grants |
Role | Lead |
Funding Start | 2017 |
Funding Finish | 2017 |
GNo | G1701164 |
Type Of Funding | Internal |
Category | INTE |
UON | Y |
20161 grants / $20,000
Superresolution fluorescence imaging in microbiology$20,000
Funding body: University of Newcastle
Funding body | University of Newcastle |
---|---|
Project Team | Professor Antoine van Oijen, Professor Nicholas Dixon, Associate Professor Cynthia Whitchurch, Associate Professor Elizabeth Harry, Dr Lynne Turnbull, Dr Till Boecking, Associate Professor Slade Jensen, Professor Ian Paulsen, Cprof PETER Lewis, Associate Professor Ian Grainge, Boecking, Dr Till, Jensen, A/Prof Slade, Turnbull, Dr Lynne |
Scheme | Equipment Grant |
Role | Investigator |
Funding Start | 2016 |
Funding Finish | 2016 |
GNo | G1500398 |
Type Of Funding | Internal |
Category | INTE |
UON | Y |
20141 grants / $10,000
DNA Replication fork stability, collapse and processing in living Escherichia coli cells$10,000
Funding body: University of Newcastle
Funding body | University of Newcastle |
---|---|
Project Team | Associate Professor Ian Grainge |
Scheme | Near Miss Grant |
Role | Lead |
Funding Start | 2014 |
Funding Finish | 2014 |
GNo | G1301382 |
Type Of Funding | Internal |
Category | INTE |
UON | Y |
20134 grants / $74,156
DVC(R) Research Support for Future Fellow (FT12)$58,012
Funding body: University of Newcastle
Funding body | University of Newcastle |
---|---|
Project Team | Associate Professor Ian Grainge |
Scheme | Future Fellowship Support |
Role | Lead |
Funding Start | 2013 |
Funding Finish | 2016 |
GNo | G1201101 |
Type Of Funding | Internal |
Category | INTE |
UON | Y |
Development of new genetic tools for protein knockouts in pathogenic bacteria$13,129
Funding body: University of Newcastle - Faculty of Science & IT
Funding body | University of Newcastle - Faculty of Science & IT |
---|---|
Project Team | Associate Professor Ian Grainge |
Scheme | Strategic Small Grant |
Role | Lead |
Funding Start | 2013 |
Funding Finish | 2013 |
GNo | G1401062 |
Type Of Funding | Internal |
Category | INTE |
UON | Y |
Faculty PVC Conference Assistance Grant 2013$2,000
Funding body: University of Newcastle - Faculty of Science & IT
Funding body | University of Newcastle - Faculty of Science & IT |
---|---|
Project Team | Associate Professor Ian Grainge |
Scheme | PVC Conference Assistance Grant |
Role | Lead |
Funding Start | 2013 |
Funding Finish | 2013 |
GNo | G1401158 |
Type Of Funding | Internal |
Category | INTE |
UON | Y |
Characterisation of the interaction between the essential bacterial transcription factor NusA and RNA Polymerase$1,015
Funding body: Australian Synchrotron
Funding body | Australian Synchrotron |
---|---|
Project Team | Associate Professor Ian Grainge |
Scheme | Travel Grant |
Role | Lead |
Funding Start | 2013 |
Funding Finish | 2013 |
GNo | G1301024 |
Type Of Funding | Other Public Sector - State |
Category | 2OPS |
UON | Y |
20123 grants / $1,099,319
Characterization of a powerful molecular motor, the FtsK DNA translocase$777,880
Funding body: ARC (Australian Research Council)
Funding body | ARC (Australian Research Council) |
---|---|
Project Team | Associate Professor Ian Grainge |
Scheme | Future Fellowships |
Role | Lead |
Funding Start | 2012 |
Funding Finish | 2016 |
GNo | G1101070 |
Type Of Funding | Aust Competitive - Commonwealth |
Category | 1CS |
UON | Y |
Targeting nucleic acid synthesis and cell division in gram-negative bacterial pathogens$311,439
Funding body: NHMRC (National Health & Medical Research Council)
Funding body | NHMRC (National Health & Medical Research Council) |
---|---|
Project Team | Professor Nicholas Dixon, Associate Professor Elizabeth Harry, Cprof PETER Lewis, Associate Professor Aaron Oakley, Associate Professor Ian Grainge |
Scheme | Project Grant |
Role | Investigator |
Funding Start | 2012 |
Funding Finish | 2014 |
GNo | G1101133 |
Type Of Funding | Aust Competitive - Commonwealth |
Category | 1CS |
UON | Y |
Ultrasonic Homogenizer System and -80 ºC Freezers for chemical and biological sample storage$10,000
Funding body: NHMRC (National Health & Medical Research Council)
Funding body | NHMRC (National Health & Medical Research Council) |
---|---|
Project Team | Professor Adam McCluskey, Professor Eileen McLaughlin, Cprof PETER Lewis, Ms Belinda Nixon, Doctor Shaun Roman, Doctor Jennette Sakoff, Associate Professor Ian Grainge |
Scheme | Equipment Grant |
Role | Investigator |
Funding Start | 2012 |
Funding Finish | 2012 |
GNo | G1100986 |
Type Of Funding | Other Public Sector - Commonwealth |
Category | 2OPC |
UON | Y |
20114 grants / $1,199,983
Molecular characterization of the role of FtsK in chromosome unlinking and segregation.$455,022
Funding body: NHMRC (National Health & Medical Research Council)
Funding body | NHMRC (National Health & Medical Research Council) |
---|---|
Project Team | Associate Professor Ian Grainge |
Scheme | Project Grant |
Role | Lead |
Funding Start | 2011 |
Funding Finish | 2013 |
GNo | G1000271 |
Type Of Funding | Aust Competitive - Commonwealth |
Category | 1CS |
UON | Y |
Chemical Biology$444,961
Funding body: University of Newcastle
Funding body | University of Newcastle |
---|---|
Project Team | Professor Adam McCluskey, Doctor Warwick Belcher, Associate Professor Ian Grainge, Professor Christopher Grof, Cprof PETER Lewis, Professor Eileen McLaughlin, Doctor Shaun Roman, Emeritus Professor Ray Rose, Doctor Jennette Sakoff, Associate Professor Nikki Verrills |
Scheme | Priority Research Centre |
Role | Investigator |
Funding Start | 2011 |
Funding Finish | 2013 |
GNo | G1100052 |
Type Of Funding | Internal |
Category | INTE |
UON | Y |
DNA Replication fork processing and recovery in living Escherichia coli cells$285,000
Funding body: ARC (Australian Research Council)
Funding body | ARC (Australian Research Council) |
---|---|
Project Team | Associate Professor Ian Grainge |
Scheme | Discovery Projects |
Role | Lead |
Funding Start | 2011 |
Funding Finish | 2013 |
GNo | G1000148 |
Type Of Funding | Aust Competitive - Commonwealth |
Category | 1CS |
UON | Y |
Eppendorf mastercycler pro with thermomixer comfort and 5430R centrifuge$15,000
Funding body: NHMRC (National Health & Medical Research Council)
Funding body | NHMRC (National Health & Medical Research Council) |
---|---|
Project Team | Professor Eileen McLaughlin, Cprof PETER Lewis, Professor Adam McCluskey, Conjoint Professor Keith Jones, Professor Brett Nixon, Doctor Shaun Roman, Doctor Jennette Sakoff, Associate Professor Ian Grainge, Doctor Janet Bristow, Doctor Xiao Yang |
Scheme | Equipment Grant |
Role | Investigator |
Funding Start | 2011 |
Funding Finish | 2011 |
GNo | G1100028 |
Type Of Funding | Other Public Sector - Commonwealth |
Category | 2OPC |
UON | Y |
20101 grants / $5,000
Chromosome stability in pathogenic bacteria$5,000
Funding body: University of Newcastle
Funding body | University of Newcastle |
---|---|
Project Team | Associate Professor Ian Grainge |
Scheme | New Staff Grant |
Role | Lead |
Funding Start | 2010 |
Funding Finish | 2010 |
GNo | G1000625 |
Type Of Funding | Internal |
Category | INTE |
UON | Y |
Research Supervision
Number of supervisions
Current Supervision
Commenced | Level of Study | Research Title | Program | Supervisor Type |
---|---|---|---|---|
2024 | PhD | Yeast Fermentation and Strain Optimisation For the Ethtec Cellulosic Ethanol Pilot Plant Project | PhD (Biological Sciences), College of Engineering, Science and Environment, The University of Newcastle | Principal Supervisor |
2023 | PhD | Toxicological implication microplastics and human health | PhD (Biological Sciences), College of Engineering, Science and Environment, The University of Newcastle | Co-Supervisor |
2023 | PhD | Microplastic and Nano Plastics Degradation in Environment via Biotechnological Approaches | PhD (Biological Sciences), College of Engineering, Science and Environment, The University of Newcastle | Co-Supervisor |
2023 | PhD | Isolation And Characterisation Of Novel Bacteriophage Against Pathogenic Bacteria | PhD (Biological Sciences), College of Engineering, Science and Environment, The University of Newcastle | Principal Supervisor |
2023 | PhD | Identifying Bacteriophage to Target Antibiotic-Resistant ESKAPE Pathogens | PhD (Biological Sciences), College of Engineering, Science and Environment, The University of Newcastle | Principal Supervisor |
2023 | PhD | Engineering Yeast Strains for Enhanced Cellulosic Bioethanol Production and High-Value Chemical Synthesis | PhD (Biological Sciences), College of Engineering, Science and Environment, The University of Newcastle | Principal Supervisor |
2017 | PhD | Developing a Yeast Biocatalyst for Efficient Fermentation of Lignocellulosic Sugars | PhD (Biological Sciences), College of Engineering, Science and Environment, The University of Newcastle | Principal Supervisor |
Past Supervision
Year | Level of Study | Research Title | Program | Supervisor Type |
---|---|---|---|---|
2023 | PhD | Type III Polyketide Biosynthesis in Cyanobacteria | PhD (Biological Sciences), College of Engineering, Science and Environment, The University of Newcastle | Co-Supervisor |
2023 | PhD | Biological Characterisation of Anthranilic Acid Holoenzyme Assembly Inhibitors | PhD (Biological Sciences), College of Engineering, Science and Environment, The University of Newcastle | Principal Supervisor |
2022 | PhD | A Study into DNA Recombination Proteins and Novel Plasmid Recombination Sites from Acinetobacter baumannii | PhD (Biological Sciences), College of Engineering, Science and Environment, The University of Newcastle | Principal Supervisor |
2021 | PhD | PlastiBiome: Unravelling the Interaction of Microorganisms with Plastics and its Environmental Implication | PhD (Biological Sciences), College of Engineering, Science and Environment, The University of Newcastle | Co-Supervisor |
2020 | PhD | When Forks Collide: Analysis of Recombination-Dependent Stable DNA Replication (RSDR) | PhD (Biological Sciences), College of Engineering, Science and Environment, The University of Newcastle | Principal Supervisor |
2019 | PhD | Understanding Chromosome Dimer Resolution Systems of Pathogenic Bacteria at a Molecular Level | PhD (Biological Sciences), College of Engineering, Science and Environment, The University of Newcastle | Principal Supervisor |
2019 | PhD | The Rescue of Replication Forks Stalled by Nucleoproteins in E. Coli | PhD (Biological Sciences), College of Engineering, Science and Environment, The University of Newcastle | Principal Supervisor |
2018 | Masters | Processing of DNA Replication Forks After Encountering a Protein Roadblock | M Philosophy (Biological Sc), College of Engineering, Science and Environment, The University of Newcastle | Principal Supervisor |
2017 | PhD | Transcription Factors and Complementation Strategies in Bacteria | PhD (Biological Sciences), College of Engineering, Science and Environment, The University of Newcastle | Co-Supervisor |
2016 | PhD | Development of Novel Genetic Tools for Molecular Investigations of Pathogenic Bacteria | PhD (Biological Sciences), College of Engineering, Science and Environment, The University of Newcastle | Principal Supervisor |
2014 | PhD | Increased Understanding of the Molecular Interactions Involved in Bacterial Transcription and Recombination | PhD (Biological Sciences), College of Engineering, Science and Environment, The University of Newcastle | Co-Supervisor |
Research Collaborations
The map is a representation of a researchers co-authorship with collaborators across the globe. The map displays the number of publications against a country, where there is at least one co-author based in that country. Data is sourced from the University of Newcastle research publication management system (NURO) and may not fully represent the authors complete body of work.
Country | Count of Publications | |
---|---|---|
Australia | 26 | |
United Kingdom | 25 | |
United States | 19 | |
Japan | 3 | |
Denmark | 2 | |
More... |
Associate Professor Ian Grainge
Position
Associate Professor
School of Environmental and Life Sciences
College of Engineering, Science and Environment
Focus area
Biological Sciences
Contact Details
ian.grainge@newcastle.edu.au | |
Phone | 4921 7238 |
Fax | 4921 5472 |
Office
Room | BG09 |
---|---|
Building | Biological Sciences |
Location | BG09, Biological Sciences, Callaghan University Drive Callaghan, NSW 2308 Australia |