Staff Profile

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Career Summary

Qualifications

  • PhD, University of Newcastle, 13/08/2005
  • Bachelor of Science (Honours), University of Newcastle, 09/12/1998
  • Bachelor of Science, University of Newcastle, 26/09/1997

Research

Research keywords

  • Analytical Chemistry
  • DNA Chemistry
  • Proteomics
  • Sperm Cell Biology

Research expertise

Geoff’s research interests involve sperm cell biology with specific focus on how reactive oxygen species negatively impact the male germ cell including the processes which lead to DNA damage. His expertise includes analytical chemistry, bio-marker development, DNA damage detection and DNA chemistry. Understanding the origins and types of DNA damage in human spermatozoa and its consequences for human fertility, are his key research questions.

Fields of Research

CodeDescriptionPercentage
111201Cancer Cell Biology50
060199Biochemistry And Cell Biology Not Elsewhere Classified40
111404Reproduction10

Memberships

Other

  • The Royal Chemistry Society

Teaching

Teaching keywords

  • Analytical Chemistry
  • Sperm Cell Biology
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Highlighted Publications

For publications that are currently unpublished or in-press, details are shown in italics.

YearCitationAltmetricsLink
2006De Iuliis GN, Wingate JK, Koppers AJ, McLaughlin EA, Aitken RJ, 'Definitive evidence for the nonmitochondrial production of superoxide anion by human spermatozoa', Journal of Clinical Endocrinology and Metabolism, 91 1968-1975 (2006) [C1]

This is a significant paper for the study of male infertility and spermatozoa.We were able to show, for the first time, that human spermatozoa can generate superoxide.

2009De Iuliis GN, Newey RJ, King BV, Aitken RJ, 'Mobile phone radiation induces reactive oxygen species production and DNA damage in human spermatozoa in vitro', PLoS ONE, 4 e6446 (2009) [C1]

A first for the field, this study showed a string of cause and effect from oxidative stress to nuclear DNA damage and motility loss, shedding light on a mechanism of action of non-ionising radiation on biology.

2009De Iuliis GN, Thomson LK, Mitchell LA, Read JM, Koppers AJ, Hedges A, et al., 'DNA damage in human spermatozoa is highly correlated with the efficiency of chromatin remodeling and the formation of 8-hydroxy-2'-deoxyguanosine, a marker of oxidative stress', Biology of Reproduction, 81 517-524 (2009) [C1]

We established an assay which detects oxidative DNA damage in human spermatozoa. We showed that a large proportion of DNA damage incurred by human spermatozoa is mediated by oxidative stress.

2008Koppers AJ, De Iuliis GN, Read JM, McLaughlin EA, Aitken RJ, 'Significance of mitochondrial reactive oxygen species in the generation of oxidative stress in spermatozoa', Journal of Clinical Endocrinology & Metabolism, 93 3199-3207 (2008) [C1]

We present conclusive evidence that human spermatozoa produced superoxide via their mitochondria which leads to a range of adverse effects.

Publications

For publications that are currently unpublished or in-press, details are shown in italics.

Click on a category title below to expand the list of citations for that specific category.

Chapter (5 outputs)

YearCitationAltmetricsLink
2012De Iuliis GN, King BV, Aitken RJ, 'Electromagnetic radiation and oxidative stress in the male germ line', Studies on Men's Health and Fertility, Springer, New York 3-20 (2012) [B1]

Co-authors: Bruce King, John Aitken

2012Aitken RJ, De Iuliis GN, Baker MA, 'Direct methods for the detection of reactive oxygen species in human semen samples', Studies on Men's Health and Fertility, Springer, New York 275-299 (2012) [B1]

Co-authors: John Aitken, Mark Baker

2011Aitken RJ, De Iuliis GN, 'Role of oxidative stress in the etiology of sperm DNA damage', Sperm Chromatin, Springer New York 277-293 (2011)
2010Aitken RJ, Baker MA, De Iuliis GN, Nixon B, 'New insights into sperm physiology and pathology', Fertility Control, Springer, Berlin 99-115 (2010) [B1]
2007Aitken RJ, Roman SD, Baker MA, De Iuliis GN, 'Redox regulation of DNA damage in the male germ line', Male-Mediated Developmental Toxicity, Royal Society of Chemistry, London, United Kingdom 197-209 (2007) [B1]
Show 2 more

Journal article (32 outputs)

YearCitationAltmetricsLink
2014Aitken RJ, Smith TB, Jobling MS, Baker MA, De Iuliis GN, 'Oxidative stress and male reproductive health', ASIAN JOURNAL OF ANDROLOGY, 16 31-38 (2014)
2014Aitken RJ, Finnie JM, Muscio L, Whiting S, Connaughton HS, Kuczera L, et al., 'Potential importance of transition metals in the induction of DNA damage by sperm preparation media.', Hum Reprod, 29 2136-2147 (2014)
2013Aitken RJ, Smith TB, Lord T, Kuczera L, Koppers AJ, Naumovski N, et al., 'On methods for the detection of reactive oxygen species generation by human spermatozoa: analysis of the cellular responses to catechol oestrogen, lipid aldehyde, menadione and arachidonic acid', Andrology, 1 192-205 (2013) [C1]
2013Aitken RJ, Bronson R, Smith TB, De Iuliis GN, 'The source and significance of DNA damage in human spermatozoa; a commentary on diagnostic strategies and straw man fallacies', Molecular Human Reproduction, 19 475-485 (2013) [C1]
2013Smith TB, De Iuliis GN, Lord T, Aitken RJ, 'The senescence-accelerated mouse prone 8 as a model for oxidative stress and impaired DNA repair in the male germ line', Reproduction, 146 253-262 (2013) [C1]
2013Lewis SEM, Aitken RJ, Conner SJ, De Iuliis G, Evenson DP, Henkel R, et al., 'The impact of sperm DNA damage in assisted conception and beyond: Recent advances in diagnosis and treatment', Reproductive BioMedicine Online, 27 325-337 (2013) [C1]
2012Aitken RJ, Gibb Z, Mitchell LA, Lambourne SR, Connaughton HS, De Iuliis GN, 'Sperm motility is lost in vitro as a consequence of mitochondrial free radical production and the generation of electrophilic aldehydes but can be significantly rescued by the presence of nucleophilic thiols', Biology of Reproduction, 87 1-11 (2012) [C1]

Co-authors: John Aitken

2012Aitken RJ, Whiting SJ, De Iuliis GN, McClymont SL, Mitchell LA, Baker MA, 'Electrophilic aldehydes generated by sperm metabolism activate mitochondrial reactive oxygen species generation and apoptosis by targeting succinate dehydrogenase', Journal of Biological Chemistry, 287 33048-33060 (2012) [C1]

We detail the mechanism of reactive oxygen species production in spermatozoa, which greatly progresses our groups previous insights into the mitochondria’s role in producing reactive oxygen species. [IF 5.1]

2012Aitken RJ, De Iuliis GN, Gibb Z, Baker MA, 'The Simmet lecture: New horizons on an old landscape - oxidative stress, DNA damage and apoptosis in the male germ line', Reproduction in Domestic Animals, 47 7-14 (2012) [C2]

Co-authors: John Aitken, Mark Baker

2011Aitken RJ, Mitchell L, De Iuliis GN, 'Reply: M540 bodies interfere with TUNEL analyses in human semen samples', HUMAN REPRODUCTION, 26 729-730 (2011) [C3]
2010Aitken RJ, De Iuliis GN, Finnie JM, Hedges A, McLachlan RI, 'Analysis of the relationships between oxidative stress, DNA damage and sperm vitality in a patient population: Development of diagnostic criteria', Human Reproduction, 25 2415-2426 (2010) [C1]
2010Mitchell LA, De Iuliis GN, Aitken RJ, 'The TUNEL assay consistently underestimates DNA damage in human spermatozoa and is influenced by DNA compaction and cell vitality: Development of an improved methodology', International Journal of Andrology, 34 2-13 (2010) [C1]
2010Aitken RJ, De Iuliis GN, 'On the possible origins of DNA damage in human spermatozoa', Molecular Human Reproduction, 16 3-13 (2010) [C1]
2009Aitken RJ, De Iuliis GN, McLachlan RI, 'Biological and clinical significance of DNA damage in the male germ line', International Journal of Andrology, 32 46-56 (2009) [C1]
2009De Iuliis GN, Newey RJ, King BV, Aitken RJ, 'Mobile phone radiation induces reactive oxygen species production and DNA damage in human spermatozoa in vitro', PLoS ONE, 4 e6446 (2009) [C1]

A first for the field, this study showed a string of cause and effect from oxidative stress to nuclear DNA damage and motility loss, shedding light on a mechanism of action of non-ionising radiation on biology.

2009Czarny NA, Harris MS, De Iuliis GN, Rodger JC, 'Acrosomal integrity, viability, and DNA damage of sperm from dasyurid marsupials after freezing or freeze drying', Theriogenology, 72 817-825 (2009) [C1]
2009De Iuliis GN, Thomson LK, Mitchell LA, Read JM, Koppers AJ, Hedges A, et al., 'DNA damage in human spermatozoa is highly correlated with the efficiency of chromatin remodeling and the formation of 8-hydroxy-2'-deoxyguanosine, a marker of oxidative stress', Biology of Reproduction, 81 517-524 (2009) [C1]

We established an assay which detects oxidative DNA damage in human spermatozoa. We showed that a large proportion of DNA damage incurred by human spermatozoa is mediated by oxidative stress.

2009Thomson LK, Fleming SD, Aitken RJ, De Iuliis GN, Zieschang JA, Clark AM, 'Cryopreservation-induced human sperm DNA damage is predominantly mediated by oxidative stress rather than apoptosis', Human Reproduction, 24 2061-2070 (2009) [C1]
2009Bennetts LE, De Iuliis GN, Nixon B, Kime M, Zelski K, McVicar CM, et al., 'Impact of estrogenic compounds on DNA integrity in human spermatozoa: Evidence for cross-linking and redox cycling activities', Journal of Urology, 181 914-915 (2009) [C3]
2008Koppers AJ, De Iuliis GN, Read JM, McLaughlin EA, Aitken RJ, 'Significance of mitochondrial reactive oxygen species in the generation of oxidative stress in spermatozoa', Journal of Clinical Endocrinology & Metabolism, 93 3199-3207 (2008) [C1]

We present conclusive evidence that human spermatozoa produced superoxide via their mitochondria which leads to a range of adverse effects.

2008Bennetts LE, De Iuliis GN, Nixon B, Kime M, Nixon K, McVicar CM, et al., 'Impact of estrogenic compounds on DNA integrity in human spermatozoa: Evidence for cross-linking and redox cycling activities', Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 641 1-11 (2008) [C1]
2007Aitken RJ, De Iuliis GN, 'Value of DNA integrity assays for fertility evaluation', Society for Reproduction and Fertility Supplement, 65 81-92 (2007) [C1]

Co-authors: John Aitken

2007Aitken RJ, Wingate JK, De Iuliis GN, McLaughlin EA, 'Analysis of lipid peroxidation in human spermatozoa using BODIPY C11', Molecular Human Reproduction, 13 203-211 (2007) [C1]
2007Aitken RJ, De Iuliis GN, 'Origins and consequences of DNA damage in male germ cells', Reproductive Biomedicine Online, 14 727-733 (2007) [C1]

This article has been cited 114 times as of 2012. It details what is known about the types of DNA damage in the male germ line and their consequences for human fertility. [IF 2.3, 114 citations]

2006Aitken RJ, Wingate JK, De Iuliis GN, Koppers AJ, McLaughlin EA, 'Cis-unsaturated Fatty acids stimulate reactive oxygen species generation and lipid peroxidation in human spermatozoa', Journal of Clinical Endocrinology and Metabolism, 91 4154-4163 (2006) [C1]
2006De Iuliis GN, Wingate JK, Koppers AJ, McLaughlin EA, Aitken RJ, 'Definitive evidence for the nonmitochondrial production of superoxide anion by human spermatozoa', Journal of Clinical Endocrinology and Metabolism, 91 1968-1975 (2006) [C1]

This is a significant paper for the study of male infertility and spermatozoa.We were able to show, for the first time, that human spermatozoa can generate superoxide.

2006McCann N, De Iuliis GN, Lawrance GA, Maeder M, Schrader K, Moore P, 'Mono- and dinuclear copper(II) complexes of pendant-arm macrocyclic polyamines: Synthesis, characterization and investigation as hydrolytic cleavage agents for DNA', Inorganic Reaction Mechanisms, 6 91-112 (2006) [C1]
2004Robertson MJ, De Iuliis GN, Maeder M, Lawrance GA, 'Metal-directed synthesis of a chiral acyclic pentaamine and pendant-arm macrocyclic hexaamine derived from an amino acid', Inorganica Chimica Acta, 357 557-570 (2004) [C1]
2003Comba P, De Iuliis GN, Lawrance GA, Luther S, Maeder M, Nolan AL, et al., 'Geometrical isomerism in octahedral complexes arising from the presence of a fused ring on a triaza macrocycle', Dalton Transactions, 2188-2193 (2003) [C1]
2003De Iuliis GN, Lawrance GA, Bernhardt PV, 'DNA cleavage with dinuclear metal complexes of ligands with varying flexibility', Journal of Inorganic Biochemistry, 96 158 (2003) [C3]

Co-authors: Geoffrey Lawrance

2002De Iuliis GN, Lawrance GA, Wilson N, 'Metal complex-promoted cleavage of RNA dimers and trimers; electrospray ionisation mass spectrometry evidence for cleavage', Inorganic Reaction Mechanisms, 4 169-186 (2002) [C1]

Co-authors: Geoffrey Lawrance

2000De Iuliis GN, Lawrance GA, Fieuw-Makaroff S, 'Superior hydrolytic DNA cleavage by a dinuclear copper(II) N4S4-donor complex compared with a mononuclear N2S2-donor close analogue', INORGANIC CHEMISTRY COMMUNICATIONS, 3 307-309 (2000) [C1]

Artificial nucleases were designed and synthesised. We proposed a 2-centred Cu(ii) complex would elevate rates of DNA hydrolytic cleavage. One compound exhibited one of the highest activities at the time. [IF 2.0, 32 citations]

Co-authors: Geoffrey Lawrance

Show 29 more

Conference (10 outputs)

YearCitationAltmetricsLink
2009Aitken RJ, De Iuliis GN, Koppers AJ, 'OXIDATIVE STRESS AND SPERMATOZOA HEALTH', JOURNAL OF ANDROLOGY, Barcelona, SPAIN (2009) [E3]
2009de Iuliis GN, Newey RJ, King BV, Aitken RJ, 'MOBILE PHONE RADIATION INDUCES REACTIVE OXYGEN SPECIES PRODUCTION AND DNA DAMAGE IN HUMAN SPERMATOZOA IN VITRO', JOURNAL OF ANDROLOGY, Barcelona, SPAIN (2009) [E3]
2008Aitken RJ, De Iuliis GN, Nixon B, Roman SD, 'Causes and clinical significance of DNA damage in the male germ line', Proceedings of 41st Annual Meeting of the Society for the Study of Reproduction. "Reproductive Biology: Basic Discoveries that Affect Our Lives", Kailua-Kona, Hawaii (2008) [E3]
2008De Iuliis GN, Nixon K, Aitken RJ, 'Estrogenic compounds induce oxidative stress in human spermatozoa: Implications for DNA integrity and fertilization success', Biology of Reproduction, Kailua-Kona, Hawaii (2008) [E3]

Co-authors: John Aitken

2007Schjenken JE, Stanger SJ, Smith V, Douglas A, De Iuliis GN, Depaz I, et al., 'Expression and activity of phase 1 detoxifying enzymes in the male sperm line of the mouse', 7th Hunter Cellular Biology Meeting. Program, Hunter Valley, NSW (2007) [E3]
2007De Iuliis GN, Zelski K, Aitken RJ, 'The Impact of Estrogenic Compounds on DNA Integrity in the Male Germ Line', Endocrine Society of Australia (ESA) & Society for Reproductive Biology (SRB) Annual Scientific Meeting. Proceedings, Christchurch, New Zealand (2007) [E3]

Co-authors: John Aitken

2007Aitken RJ, De Iuliis GN, 'Midnight's broken toll: The male germ cell and its role in the aetiology of genetic disease', Early Human Development, Perth, W.A. (2007) [E3]

Co-authors: John Aitken

2006Aitken RJ, De Iuliis GN, 'Value of DNA integrity assays for fertility evaluation', Abstracts, Madrid, Spain (2006) [E3]

Co-authors: John Aitken

2004De Iuliis GN, MacDougall IJA, Griffith R, 'Molecular modelling of alpha 1b and alpha 1d adrenoceptors and investigations into the effects of water and adrenaline', MM2004: 9th Molecular Modelling Workshop, Sydney, Australia (2004) [E3]
2003De Iuliis GN, Griffith R, 'Molecular Dynamics Studies On A Model Of The a1b-Adrenergic Receptor: The Effects Of Water, Adrenaline And Site Directed Mutagenesis', TOMECS International Symposium AIMECS 03 Post Symposium Meeting Abstracts, Sunforte, Japan (2003) [E3]
Show 7 more
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Grants and Funding

Summary

Number of grants2
Total funding$29,000

- Indicates that the researcher may be seeking students for this project.

Click on a grant title below to expand the full details for that specific grant.

2013 (1 grants)

NucleoCounter SP100$14,000
Funding Body: NHMRC (National Health & Medical Research Council)

Project Team
Laureate Professor John Aitken, Associate Professor Brett Nixon, Doctor Shaun Roman, Doctor Mark Baker, Doctor Geoffry De Iuliis, Doctor Lisa Mitchell, Doctor Matthew Jobling, Doctor Zamira Gibb, Mr Nenad Naumovski
SchemeRole
Equipment GrantInvestigator
Total AmountFunding StartFunding Finish
$14,00020132013
GNo:G1201175

2012 (1 grants)

BMG SPECTROstar Nano$15,000
Funding Body: NHMRC (National Health & Medical Research Council)

Project Team
Laureate Professor John Aitken, Professor Eileen McLaughlin, Ms Belinda Nixon, Doctor Shaun Roman, Doctor Mark Baker, Doctor Geoffry De Iuliis, Doctor Lisa Mitchell, Doctor Matthew Jobling
SchemeRole
Equipment GrantInvestigator
Total AmountFunding StartFunding Finish
$15,00020122012
GNo:G1100971
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Research Supervision

Number of current supervisions1

For supervisions undertaken at an institution other that the University of Newcastle, the institution name is listed below the program name.

Current Supervision

CommencedProposed
Completion
ProgramSupervisor TypeResearch Title
20062007Honours Biol Sc Not Elsewhere Classifd
University of Newcastle
Co-SupervisorDNA Damage in the Male Germ Line

Past Supervision

YearProgramSupervisor TypeResearch Title
2006Honours Biol Sc Not Elsewhere Classifd
University of Newcastle
Co-SupervisorDNA Damage in the Male Germ Line
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Dr Geoffry De Iuliis

Work Phone(02) 4921 7295
Fax(02) 4921 6308
Email
PositionResearch Fellow
School of Biomedical Sciences and Pharmacy
Faculty of Health and Medicine
The University of Newcastle, Australia
Focus AreaReproductive Medicine
Office
LS3-39,
Life Sciences,
Callaghan
University Drive
Callaghan NSW 2308
Australia
URL:www.newcastle.edu.au/profile/geoffry-deiuliis