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Dr Geoffrey MacFarlane

Senior Lecturer

School of Environmental and Life Sciences (Environmental Science and Management)

Career Summary

Biography

Research Expertise
My principal research interests fall broadly into two main areas -Estuarine and Marine Ecotoxicology -Behavioural Ecology & Animal Behaviour Research support through Australian Research Council Discovery and Linkage Grants 2004, 2004, 2006, 2009

Teaching Expertise
-Vice Chancellors Early Career Academic Teaching Excellence Award (2005) -Australian College of Educators and NSW Department of Education and Trainings Quality Teaching Award (2005) -NUPSA postgraduate supervisor of the year at the University of Newcastle(2006) -CARRICK institute of teaching and learning citation for outstanding contribution to student learning(2007)

Administrative Expertise
Deputy Chair, Animal Care and Ethics Committee, University of Newcastle


Qualifications

  • PhD, University of Technology Sydney
  • Graduate Certificate of Higher Education, University of Technology Sydney
  • Bachelor of Science, University of Newcastle

Keywords

  • Estuarine and Marine Ecotoxicology

Fields of Research

Code Description Percentage
050299 Environmental Science and Management not elsewhere classified 15
060799 Plant Biology not elsewhere classified 30
060899 Zoology not elsewhere classified 55

Professional Experience

UON Appointment

Title Organisation / Department
Senior Lecturer University of Newcastle
School of Environmental and Life Sciences
Australia

Academic appointment

Dates Title Organisation / Department
1/01/2003 - 1/01/2007 Lecturer University of Newcastle
School of Environmental and Life Sciences
Australia
2/03/2001 - 1/01/2003 Associate Lecturer University of Newcastle
School of Environmental and Life Sciences
Australia
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Publications

For publications that are currently unpublished or in-press, details are shown in italics.


Journal article (43 outputs)

Year Citation Altmetrics Link
2016 Tran TKA, MacFarlane GR, Kong RYC, O'Connor WA, Yu RMK, 'Mechanistic insights into induction of vitellogenin gene expression by estrogens in Sydney rock oysters, Saccostrea glomerata', Aquatic Toxicology, 174 146-158 (2016)

© 2016 Elsevier B.V.Marine molluscs, such as oysters, respond to estrogenic compounds with the induction of the egg yolk protein precursor, vitellogenin (Vtg), availing a biomark... [more]

© 2016 Elsevier B.V.Marine molluscs, such as oysters, respond to estrogenic compounds with the induction of the egg yolk protein precursor, vitellogenin (Vtg), availing a biomarker for estrogenic pollution. Despite this application, the precise molecular mechanism through which estrogens exert their action to induce molluscan vitellogenesis is unknown. As a first step to address this question, we cloned a gene encoding Vtg from the Sydney rock oyster Saccostrea glomerata (sgVtg). Using primers designed from a partial sgVtg cDNA sequence available in Genbank, a full-length sgVtg cDNA of 8498 bp was obtained by 5'- and 3'-RACE. The open reading frame (ORF) of sgVtg was determined to be 7980 bp, which is substantially longer than the orthologs of other oyster species. Its deduced protein sequence shares the highest homology at the N- and C-terminal regions with other molluscan Vtgs. The full-length genomic DNA sequence of sgVtg was obtained by genomic PCR and genome walking targeting the gene body and flanking regions, respectively. The genomic sequence spans 20 kb and consists of 30 exons and 29 introns. Computer analysis identified three closely spaced half-estrogen responsive elements (EREs) in the promoter region and a 210-bp CpG island 62 bp downstream of the transcription start site. Upregulation of sgVtg mRNA expression was observed in the ovaries following in vitro (explants) and in vivo (tank) exposure to 17ß-estradiol (E2). Notably, treatment with an estrogen receptor (ER) antagonist in vitro abolished the upregulation, suggesting a requirement for an estrogen-dependent receptor for transcriptional activation. DNA methylation of the 5' CpG island was analysed using bisulfite genomic sequencing of the in vivo exposed ovaries. The CpG island was found to be hypomethylated (with 0-3% methylcytosines) in both control and E2-exposed oysters. However, no significant differential methylation or any correlation between methylation and sgVtg expression levels was observed. Overall, the results support the possible involvement of an ERE-containing promoter and an estrogen-activated receptor in estrogen signalling in marine molluscs.

DOI 10.1016/j.aquatox.2016.02.023
Citations Scopus - 1Web of Science - 1
Co-authors Richard Yu
2016 Tran TKA, MacFarlane GR, Kong RYC, O'Connor WA, Yu RMK, 'Potential mechanisms underlying estrogen-induced expression of the molluscan estrogen receptor (ER) gene', Aquatic Toxicology, 179 82-94 (2016)

© 2016 Elsevier B.V.In vertebrates, estrogens and estrogen mimicking chemicals modulate gene expression mainly through a genomic pathway mediated by the estrogen receptors (ERs).... [more]

© 2016 Elsevier B.V.In vertebrates, estrogens and estrogen mimicking chemicals modulate gene expression mainly through a genomic pathway mediated by the estrogen receptors (ERs). Although the existence of an ER orthologue in the mollusc genome has been known for some time, its role in estrogen signalling has yet to be deciphered. This is largely due to its constitutive (ligand-independent) activation and a limited mechanistic understanding of its regulation. To fill this knowledge gap, we cloned and characterised an ER cDNA (sgER) and the 5'-flanking region of the gene from the Sydney rock oyster Saccostrea glomerata. The sgER cDNA is predicted to encode a 477-amino acid protein that contains a DNA-binding domain (DBD) and a ligand-binding domain (LBD) typically conserved among both vertebrate and invertebrate ERs. A comparison of the sgER LBD sequence with those of other ligand-dependent ERs revealed that the sgER LBD is variable at several conserved residues known to be critical for ligand binding and receptor activation. Ligand binding assays using fluorescent-labelled E2 and purified sgER protein confirmed that sgER is devoid of estrogen binding. In silico analysis of the sgER 5'-flanking sequence indicated the presence of three putative estrogen responsive element (ERE) half-sites and several putative sites for ER-interacting transcription factors, suggesting that the sgER promoter may be autoregulated by its own gene product. sgER mRNA is ubiquitously expressed in adult oyster tissues, with the highest expression found in the ovary. Ovarian expression of sgER mRNA was significantly upregulated following in vitro and in vivo exposure to 17ß-estradiol (E2). Notably, the activation of sgER expression by E2 in vitro was abolished by the specific ER antagonist ICI 182, 780. To determine whether sgER expression is epigenetically regulated, the in vivo DNA methylation status of the putative proximal promoter in ovarian tissues was assessed using bisulfite genomic sequencing. The results showed that the promoter is predominantly hypomethylated (with 0¿3.3% methylcytosines) regardless of sgER mRNA levels. Overall, our investigations suggest that the estrogen responsiveness of sgER is regulated by a novel ligand-dependent receptor, presumably via a non-genomic pathway(s) of estrogen signalling.

DOI 10.1016/j.aquatox.2016.08.015
Co-authors Richard Yu
2016 Grace EJ, MacFarlane GR, 'Assessment of the bioaccumulation of metals to chicken eggs from residential backyards', Science of the Total Environment, 563-564 256-260 (2016)

© 2016 Elsevier B.V.Soil in urban areas contains the residues of past land-uses and practices. Urban farming (keeping chickens, vegetable gardening) requires soil disturbance and... [more]

© 2016 Elsevier B.V.Soil in urban areas contains the residues of past land-uses and practices. Urban farming (keeping chickens, vegetable gardening) requires soil disturbance and can increase exposure of residents to these contaminants. We measured the level of lead, arsenic, cadmium, copper and zinc contaminants in soil and eggs from 26 backyard chicken coops across the Lower Hunter, NSW Australia. We compared the levels of metals in soil to Health Investigation Levels and metals in home-grown eggs to the levels in commercial eggs tested in this study or published by Food Standards Australia New Zealand. The levels of arsenic, cadmium, copper and zinc were low, both in soil and in home-grown eggs and were comparable to commercial eggs tested in this study. The Health Investigation Level for lead in soil (300 mg lead/kg soil) was exceeded at 7 of the 26 sites. The level of lead in home-grown eggs was generally higher than in commercial eggs. The reference health standard for meat (including chicken), fruit and vegetables of 0.1 mg lead/kg produce was exceeded in home-grown eggs from 7 of the 26 sites. There was a significant relationship between the lead level in eggs and the lead level in soil accessible to chickens. As soil lead increased, concentrations of lead in eggs tended to increase. No relationship was detected between the lead level in feed and in eggs. We recommend strategies to reduce ingestion of soil by chickens thereby reducing metal contamination in home-grown eggs.

DOI 10.1016/j.scitotenv.2016.04.128
Citations Scopus - 1
2016 MacFarlane GR, Vasey PL, 'Promiscuous primates engage in same-sex genital interactions', Behavioural Processes, 126 21-26 (2016) [C1]

© 2016 Elsevier B.V.Same-sex genital interactions (SSGIs) occur across the order primates, yet explaining their maintenance in evolutionary terms appears problematic; as such int... [more]

© 2016 Elsevier B.V.Same-sex genital interactions (SSGIs) occur across the order primates, yet explaining their maintenance in evolutionary terms appears problematic; as such interactions seem to counteract reproductive goals. We hypothesised that in more promiscuous species, where sexual motivation, mating effort, and non-conceptive heterosexual behaviour are greater, SSGIs may also occur at greater frequencies without necessarily impeding reproduction. We found that the expression of both male and female SSGIs were greater in multimale systems than in unimale ones. Both male and female SSGIs were positively correlated with the degree of promiscuity (relative testes mass). As mating system confers biases in the sex ratio that may influence the expression of SSGIs, we controlled for availability of members of the same-sex. When employing this control, results were largely congruent. For males, SSGIs were expressed more frequently in multimale systems. For both sexes, SSGIs were expressed more frequently with greater relative testes mass. We suggest SSGIs in primates may be a neutral by-product of selection for increases in promiscuous sexual activity, and that in certain instances these interactions may be co-opted to facilitate adaptive social functions.

DOI 10.1016/j.beproc.2016.02.016
Citations Scopus - 1Web of Science - 1
2012 Andrew-Priestley MN, O'Connor WA, Dunstan RH, Van Zwieten L, Tyler T, Kumar A, Macfarlane GR, 'Estrogen mediated effects in the Sydney rock oyster, Saccostrea glomerata, following field exposures to sewage effluent containing estrogenic compounds and activity', Aquatic Toxicology, 120-121 99-108 (2012) [C1]
Citations Scopus - 11Web of Science - 11
Co-authors Hugh Dunstan
2011 Jelbart JE, Schreider MJ, Macfarlane GR, 'An investigation of benthic sediments and macrofauna within pearl farms of Western Australia', Aquaculture, 319 466-478 (2011) [C1]
DOI 10.1016/j.aquaculture.2011.07.011
Citations Scopus - 4Web of Science - 3
Co-authors Maria Schreider
2010 Griffin AS, Boyce HM, Macfarlane GR, 'Social learning about places: Observers may need to detect both social alarm and its cause to learn', Animal Behaviour, 79 459-465 (2010) [C1]
DOI 10.1016/j.anbehav.2009.11.029
Citations Scopus - 5Web of Science - 5
Co-authors Andrea Griffin
2010 Macfarlane GR, Blomberg SP, Vasey PL, 'Homosexual behaviour in birds: Frequency of expression is related to parental care disparity between the sexes', Animal Behaviour, 80 375-390 (2010) [C1]
DOI 10.1016/j.anbehav.2010.05.009
Citations Scopus - 15Web of Science - 16
2010 Winning GB, Macfarlane GR, 'Establishment of Sarcocornia Quinqueflora and Sporobolus Virginicus in a created saltmarsh: Species-specific responses to topsoil addition and assisted planting', Wetlands (Australia), 26 1-14 (2010) [C1]
2010 Priestley MN, O'Connor WA, Dunstan RH, Macfarlane GR, 'Exposure to 17 a-ethynylestradiol causes dose and temporally dependent changes in intersex, females and vitellogenin production in the Sydney rock oyster', Ecotoxicology, 19 1440-1451 (2010) [C1]
DOI 10.1007/s10646-010-0529-5
Citations Scopus - 19Web of Science - 17
Co-authors Hugh Dunstan
2010 Howe A, Rodriguez JF, Spencer J, Macfarlane GR, Saintilan N, 'Response of estuarine wetlands to reinstatement of tidal flows', Marine and Freshwater Research, 61 702-713 (2010) [C1]
DOI 10.1071/MF09171
Citations Scopus - 8Web of Science - 9
Co-authors Jose Rodriguez
2009 Gifford S, Macfarlane GR, Koller CE, Dunstan RH, O'Connor W, 'Zooremediation of contaminated aquatic systems through aquaculture initiatives 750-768 (2009)

The ability of animals to act in a bioremediative capacity is not widely known. Animals are rarely considered for bioremediation initiatives due largely to ethical or human health... [more]

The ability of animals to act in a bioremediative capacity is not widely known. Animals are rarely considered for bioremediation initiatives due largely to ethical or human health concerns. Nonetheless, specific examples in the literature reveal that many aquatic species, including species employed in aquaculture, are effective remediators of metals, microbial contaminants, hydrocarbons, nutrients and persistent organic pollutants. We introduce zoological equivalents of the definitions used in the phytoremediation literature (zooextraction, zootransformation, zoostabilisation and animal hyperaccumulation), to serve as useful benchmarks in the evaluation of candidate animal species for zooremediation initiatives. Further, we present a case study assessing the deployment of pearl oysters to remove metals and nutrients from aquatic ecosystems. © 2009 Woodhead Publishing Limited. All rights reserved.

DOI 10.1533/9781845696474.4.750
Co-authors Hugh Dunstan
2009 McLean CM, Koller CE, Rodger JC, Macfarlane GR, 'Mammalian hair as an accumulative bioindicator of metal bioavailability in Australian terrestrial environments', Science of the Total Environment, 407 3588-3596 (2009) [C1]
DOI 10.1016/j.scitotenv.2009.01.038
Citations Scopus - 24Web of Science - 20
Co-authors John Rodger
2009 Greenwood ME, Macfarlane GR, 'Effects of salinity on competitive interactions between two Juncus species', Aquatic Botany, 90 23-29 (2009) [C1]
DOI 10.1016/j.aquabot.2008.05.001
Citations Scopus - 16Web of Science - 13
2008 Koller CE, Patrick JW, Rose RJ, Offler CE, Macfarlane GR, 'Arsenic and heavy metal accumulation by Pteris vittata L. and P-umbrosa R. Br', Bulletin of Environmental Contamination and Toxicology, 80 128-133 (2008) [C1]
DOI 10.1007/s00128-007-9330-4
Citations Scopus - 5Web of Science - 3
Co-authors Tina Offler, John Patrick, Ray Rose
2008 Caregnato FF, Koller CE, Macfarlane GR, Moreira JCF, 'The glutathione antioxidant system as a biomarker suite for the assessment of heavy metal exposure and effect in the grey mangrove, Avicennia marina (Forsk.) Vierh', Marine Pollution Bulletin, 56 1119-1127 (2008) [C1]
DOI 10.1016/j.marpolbul.2008.03.019
Citations Scopus - 31Web of Science - 24
2008 Priestley MN, Dunstan RH, O'Connor WA, Van Zwieten L, Nixon B, Macfarlane GR, 'Effects of 4-nonylphenol and 17 alpha-ethynylestradiol exposure in the Sydney rock oyster, Saccostrea glomerata: Vitellogenin induction and gonadal development', Aquatic Toxicology, 88 39-47 (2008) [C1]
DOI 10.1016/j.aquatox.2008.03.003
Citations Scopus - 40Web of Science - 37
Co-authors Hugh Dunstan, Brett Nixon
2007 Macfarlane GR, Blomberg SP, Kaplan G, Rogers LJ, 'Same-sex sexual behavior in birds: expression is related to social mating system and state of development at hatching', Behavioral Ecology, 18 21-33 (2007) [C1]
DOI 10.1093/beheco/arl065
Citations Scopus - 26Web of Science - 21
2007 Macfarlane GR, 'Birds of a feather', Australasian Science, 16-17 (2007) [C2]
2007 Koller CE, Patrick JW, Rose RJ, Offler CE, Macfarlane GR, 'Pteris umbrosa R.Br. as an arsenic hyperaccumulator: accumulation, partitioning and comparison with the established As hyperaccumulator Pteris vittata', Chemosphere, 66 1256-1263 (2007) [C1]
DOI 10.1016/j.chemosphere.2006.07.029
Citations Scopus - 15Web of Science - 11
Co-authors Tina Offler, John Patrick, Ray Rose
2007 Macfarlane GR, Koller CE, Blomberg SP, 'Accumulation and partitioning of heavy metals in mangroves: A synthesis of field-based studies', Chemosphere, 69 1454-1464 (2007) [C1]
DOI 10.1016/j.chemosphere.2007.04.059
Citations Scopus - 90Web of Science - 73
2007 Gifford SP, Dunstan RH, O'Connor W, Koller CE, Macfarlane GR, 'Aquatic zooremediation: deploying animals to remediate contaminated aquatic environments', Trends in Biotechnology, 25 60-65 (2007) [C1]
DOI 10.1016/j.tibtech.2006.12.002
Citations Scopus - 33Web of Science - 20
Co-authors Hugh Dunstan
2006 Macfarlane GR, Markwell KW, Date-Huxtable EM, 'Modelling the research process as a deep learning strategy', Journal of Biological Education, 41 13-20 (2006) [C1]
DOI 10.1080/00219266.2006.9656051
Citations Scopus - 7Web of Science - 4
2006 Macfarlane GR, Schreider MJ, McLennan BS, 'Biomarkers of Heavy Metal Contamination in the Red Fingered Marsh Crab, Parasesarma erythodactyla', Archives of Environmental Contamination and Toxicology, 51 584-593 (2006) [C1]
DOI 10.1007/s00244-005-5067-4
Citations Scopus - 11Web of Science - 11
Co-authors Maria Schreider
2006 Macfarlane GR, Markich SJ, Linz K-B, Gifford SP, Dunstan RH, O'Connor W, Russell RA, 'The Akoya pearl oyster shell as an archival monitor of lead exposure', Environmental Pollution, 143 166-173 (2006) [C1]
DOI 10.1016/j.envpol.2005.10.042
Citations Scopus - 9Web of Science - 7
Co-authors Hugh Dunstan
2006 Greenwood ME, Macfarlane GR, 'Effects of salinity and temperature on the germination of Phragmites australis, Juncus kraussii, and Juncus acutus: Implications for estuarine restoration initiatives', Wetlands, 26 854-861 (2006) [C1]
DOI 10.1672/0277-5212(2006)26[854:EOSATO]2.0.CO;2
Citations Scopus - 27Web of Science - 27
2006 Gifford SP, Macfarlane GR, O'Connor WA, Dunstan RH, 'Effect of the pollutants lead, zinc, hexadecane and octocosane on total growth and shell growth in the Akoya pearl oyster, Pinctada imbricata', Journal of Shellfish Research, 25 159-165 (2006) [C1]
DOI 10.2983/0730-8000(2006)25[159:EOTPLZ]2.0.CO;2
Citations Scopus - 7Web of Science - 6
Co-authors Hugh Dunstan
2005 Gifford SP, Dunstan RH, O'Connor W, Macfarlane GR, 'Quantification of in situ nutrient and heavy metal remediation by a small pearl oyster (Pinctada imbricata) farm at Port Stephens, Australia', Marine Pollution Bulletin, 50 417-422 (2005) [C1]
DOI 10.1016/j.marpolbul.2004.11.024
Citations Scopus - 29Web of Science - 25
Co-authors Hugh Dunstan
2004 Macfarlane GR, Reid DJ, Eckersley CE, 'Sublethal Behavioural Effects of the Water Accommodated Fractions of Crude Oil to Gastropod Molluscs', Bulletin of Environmental Contamination and Toxicology, 72 1025-1031 (2004) [C1]
DOI 10.1007/s00128-004-0346-8
Citations Scopus - 4Web of Science - 2
2004 Macfarlane GR, Markwell KW, 'Homosexuals, naturally', Nature Australia, 27 52-60 (2004) [C3]
2004 Macfarlane GR, 'Oyster Plan for Toxic Waste', Australasian Science, 25 18-19 (2004) [C3]
2003 Macfarlane GR, 'Chlorophyll a Fluorescence as a Potential Biomarker of Zinc Stress in the Grey Mangrove, Avicennia marina (Forsk.) Vierh', Bulletin of Environmental Contamination and Toxicology, 90-96 (2003) [C1]
DOI 10.1007/s00128-002-0160-0
Citations Scopus - 31Web of Science - 30
2003 Reid DJ, Macfarlane GR, 'Potential biomarkers of crude oil exposure in the gastropod mollusc, Austrocochlea porcata: laboratory and manipulative field studies', Environmental Pollution, 147-155 (2003) [C1]
DOI 10.1016/S0269-7491(03)00209-4
Citations Scopus - 31Web of Science - 29
2003 Macfarlane GR, Pulkownik A, Burchett MD, 'Accumulation and distribution of heavy metals in the grey mangrove, Avicennia marina (Forsk.A) Fierh: biological indication potential', Environmental Pollution, 139-151 (2003) [C1]
DOI 10.1016/S0269-7491(02)00342-1
Citations Scopus - 108Web of Science - 91
2003 Macfarlane GR, Burchett MD, 'Assessing effects of petroleum oil on intertidal invertebrate communities in Sydney Harbour: preparedness pays off', Australasian Journal of Ecotoxicology, 9 29-38 (2003) [C1]
2002 Macfarlane GR, 'Leaf biochemical parameters as potential biomarkers of heavy metal stress in the grey mangrove, Avicennia marina (Forsk.)', Marine Pollution Bulletin, 44 244-256 (2002) [C1]
2002 Macfarlane GR, Burchett M, 'Toxicity, growth and accumulation relationships of copper, lead and zinc in the grey mangrove, Avicennia marine (Forsk.) Vierh', Marine Environmental Research, 54 65-84 (2002) [C1]
Citations Scopus - 127Web of Science - 96
2002 Macfarlane GR, 'Potential biological indicators of heavy metal stress in mangrove ecosystems.', Wetlands, 20 29-42 (2002) [C1]
2002 Macfarlane GR, 'Non-destructive sampling techniques for the rapid assessment of population parameters in estuarine shore crabs', Wetlands (Australia), 20 49-54 (2002) [C1]
2002 MacFarlane GR, 'Leaf biochemical parameters in Avicennia marina (Forsk.) Vierh as potential biomarkers of heavy metal stress in estuarine ecosystems', MARINE POLLUTION BULLETIN, 44 244-256 (2002)
DOI 10.1016/S0025-326X(01)00255-7
Citations Scopus - 50Web of Science - 44
2002 Macfarlane GR, King S, 'Observer presence influences the behaviour of the Semaphore crab, Heloecious cordiformis', Animal Behaviour, 63 1191-1194 (2002) [C1]
Citations Scopus - 10Web of Science - 11
2001 MacFarlane GR, Burchett MD, 'Photosynthetic pigments and peroxidase activity as indicators of heavy metal stress in the grey mangrove, Avicennia marina (Forsk.) Vierh', Marine Pollution Bulletin, 42 233-240 (2001) [C1]

Mangroves have been observed to possess a tolerance to high levels of heavy metals, yet accumulated metals may induce subcellular biochemical changes, which can impact on processe... [more]

Mangroves have been observed to possess a tolerance to high levels of heavy metals, yet accumulated metals may induce subcellular biochemical changes, which can impact on processes at the organism level. Six month-old seedlings of the grey mangrove, Avicennia marina (Forsk.) Vierh, were exposed to a range of Cu (0-800 µg/g), Pb (0-800 µg/g) and Zn (0-1000 µg/g) concentrations in sediments under laboratory conditions, to determine leaf tissue metal accumulation patterns, effects on photosynthetic pigments (chlorophyll a, chlorophyll b and carotenoids), and the activity of the antioxidant enzyme peroxidase. Limited Cu uptake to leaves was observed at low sediment Cu levels, with saturation and visible toxicity to Cu at sediment levels greater than 400 µg/g. Leaf Pb concentrations remained low over a range of Pb sediment concentrations, up to 400 µg/g Pb, above which it appeared that unrestricted transport of Pb occurred, although no visible signs of Pb toxicity were observed. Zn was accumulated linearly with sediment zinc concentration, and visible toxicity occurring at the highest concentration, 1000 µg/g Zn. Significant increases in peroxidase activity and decreases in photopigments were found with Cu and Zn at concentrations lower than those inducing visible toxicity. Significant increases in peroxidase activity only, were found when plants were exposed to Pb. Positive linear relationships between peroxidase activity and leaf tissue metal concentrations were found for all metals. Significant linear decreases in photosynthetic pigments with increasing leaf tissue metal concentrations were observed with Cu and Zn only. Photosynthetic pigments and peroxidase activity may be applicable as sensitive biological indicators of Cu and Zn stress, and peroxidase activity for Pb stress in A. marina. Copyright © 2001 Elsevier Science Ltd.

DOI 10.1016/S0025-326X(00)00147-8
Citations Scopus - 144
2001 MacFarlane GR, Booth DJ, 'Estuarine macrobenthic community structure in the Hawkesbury River, Australia: Relationships with sediment physicochemical and anthropogenic parameters', Environmental Monitoring and Assessment, 72 51-78 (2001) [C1]

Macrobenthic community assemblage diversity and abundance were monitored on both Cowan and Berowra Creeks in the estuarine reaches of the Hawkesbury River, Sydney Australia during... [more]

Macrobenthic community assemblage diversity and abundance were monitored on both Cowan and Berowra Creeks in the estuarine reaches of the Hawkesbury River, Sydney Australia during 1997-1998. Natural sediment physicochemical differences were assessed, along with low-level anthropogenic contaminants including copper, lead, zinc, phosphorus and nitrogen. Contaminant levels at all sites were below sediment guideline values for biological effects. Natural physicochemical sediment differences were the main determinants in species assemblage patterns among sites. Three groupings of sites with similar assemblages were observed during February 1998. Berowra Creek sites, which were higher in organic content and silt/clay (and thus metals and nutrients), higher in pH and lower in salinity, were similar in terms of contributions by the polychaetes Ceratoneresis aequisetis, Scoloplos normalis, the isopod Cyathura hakea and the bivalve mollusc Soletellina alba. Sites on upper Cowan Creek, higher in organic content, silt/clay (nutrients and metals) and lower in salinity, were similar and separated from other sites in terms of the polychaete Carazziella victoriensis and the gastropod mollusc Nassarius jonasii. Sites lower in Cowan Creek, tended to be higher in sand content, more saline and lower in organic content and thus nutrient and metal concentrations. These sites were similar in terms of contributions of the polychaete Sigalion bandaensis and the bivalve molluscs, Mysella vitrea and Tellina deltoidalis. The biotic assemblage patterns were not maintained temporally, suggesting the importance of monitoring over time to assess possible future impacts. Aggregating species data to the family level resulted in similar site discrimination. Site differences were less distinct at higher taxonomic levels and suggests future monitoring at the family level is sufficient to detect assemblage differences among sites. The results obtained represent the difficulty in detecting responses to low-level contamination at the community level, and provide a sound anticipatory baseline for the assessment of future possible anthropogenic disturbance in the Hawkesbury River.

DOI 10.1023/A:1011959721146
Citations Scopus - 21
Show 40 more journal articles

Review (1 outputs)

Year Citation Altmetrics Link
2008 Markwell KW, Macfarlane GR, 'Mangrove', The Encyclopedia of Tourism and Recreation in Marine Environments (2008) [D2]

Conference (52 outputs)

Year Citation Altmetrics Link
2010 Priestley MN, Dunstan RH, O'Connor W, Van Zweiten R, Yu MKR, Macfarlane GR, 'Molluscan bio-monitor for quantification and impcat assessment of estrogenically active compounds in Australian marine ecosystems', 20th SETAC Europe Annual Meeting, Seville, Spain: Science and Technology Environmental Protection: Programme Book (2010) [E3]
Co-authors Hugh Dunstan, Richard Yu
2008 Priestley MN, Dunstan RH, O'Connor W, Macfarlane GR, 'Assessing the dose-response relationship between 17 [alpha symbol]-ethynylestradiol, vitellogenin and effects on gonad development in the Sydney rock oyster, Saccostrea glomerata', 5th SETAC World Congress. Abstracts (2008) [E3]
Co-authors Hugh Dunstan
2008 Caregnato FF, Koller CE, Moreira JCF, Macfarlane GR, 'Oxidative stress biomarkers for heavy metal exposure and effect in the Grey Mangrove', 5th SETAC World Congress. Abstracts (2008) [E3]
2008 McLean CM, Koller CE, Macfarlane GR, 'Examining the utility of mammalian and marsupial hair as an accumulative bioindicator tissue of metal bioavailability in Australian terrestrial ecosystems', 5th SETAC World Congress. Abstracts (2008) [E3]
2008 Peak RA, Macfarlane GR, Clulow J, Mahony MJ, 'Investigations into the synchronous calling behaviour of the hip-pocket frog Assa darlingtoni (ANURA: Myobatrachidae)', 6th World Congress of Herpetology CD-ROM (2008) [E3]
Co-authors John Clulow
2007 Greenwood ME, Macfarlane GR, Toia RF, 'A field comparison of salinity stress indicators in phragmites Australis', 2007 SWIS International Conference. Water, Wetlands, and Wildlife - Resolving Conflicts and Restoring Habitat. Final Program and Abstracts (2007) [E3]
2007 Priestley MN, Dunstan RH, O'Connor WA, Van Zwieten L, Macfarlane GR, 'The Sydney Rock Oyster, Saccostrea glomerata, as a potential biomonitoring species for estrogenically active contaminants: Vitellogenin induction and gonadal development', 2nd Australasian Symposium on Ecological Risk Assessment and Management of Endocrine Disrupting Chemicals (EDCs), Pharmaceuticals and Personal Care Products (PPCPs) in the Australasian Environment (2007) [E3]
Co-authors Hugh Dunstan
2007 Jelbart JE, Dixon K, Greenwood ME, Schreider MJ, Prince J, Gifford SP, Macfarlane GR, 'Monitoring for potential impacts of pearl oyster aquaculture on marine benthos', 42nd European Marine Biology Symposium. Abstracts (2007) [E3]
Co-authors Maria Schreider
2007 Macfarlane GR, Koller CE, Caregnato F, 'Predictive biomarkers of heavy metal stress in the grey mangrove, Avicennia Marina', 5th International Conference on Marine Pollution and Ecotoxicology. Programme and Abstracts (2007) [E3]
2007 Macfarlane GR, Priestley MN, Dunstan RH, O'Connor W, Van Zwieten L, 'Vitellogenin as a predictive biomarker of exposure to the estrogenically active compounds nonylphenol and ethynylestradiol, in the Sydney Rock Oyster', 5th International Conference on Marine Pollution and Ecotoxicology. Programme and Abstracts (2007) [E3]
Co-authors Hugh Dunstan
2007 Howe A, Rodriguez JF, Spencer J, Macfarlane GR, Saintilan N, 'Effect of hydraulic manipulation on migratory shorebird habitat at an Australian coastal wetland', 6th International Ecohydraulics Symposium. Proceedings (2007) [E3]
Co-authors Jose Rodriguez
2007 Howe A, Rodriguez JF, Macfarlane GR, 'Optimising shorebird roost habitat by hydraulic manipulation', Australasian Shorebird Conference. Abstracts (2007) [E3]
Co-authors Jose Rodriguez
2007 Dixon K, Jelbart J, Prince J, Schreider M, Gifford S, Macfarlane GR, 'Assessing the impact of pearl farms on polycheate assemblages', Australian Marine Science Association conference (2007) [E1]
2007 Caregnato F, Koller CE, Moreira JCF, Macfarlane GR, 'Oxidative stress biochemistry: Potential biomarkers of heavy metal contamination in the Grey Mangrove, Avicennia marina (Forsk.) Vierh', Free Radicals in Montevideo. V Meeting of SFRBM - South American Group. V International Conference on Peroxynitrite and Reactive Nitrogen Species. Book of Abstracts (2007) [E3]
2007 Dixon K, Jelbart JE, Gifford SP, Schreider MJ, Greenwood ME, Prince J, Macfarlane GR, 'The impact of pearl aquaculture on polychaete assemblages in Western Australia', Marine Science in a Changing World (2007) [E3]
Co-authors Maria Schreider
2007 Jelbart J, Prince J, Schreider M, Dixon K, Macfarlane GR, 'Monitoring for potential impacts of pearl oyster aquaculture on marine benthos' (2007) [E3]
2006 Greenwood ME, Macfarlane GR, 'Effects of Salinity on the Germination and Growth of Three Dominant Marsh Marcrophytes', Conference Proceedings and Handbook (2006) [E1]
2006 Linz K-B, Macfarlane GR, Dunstan RH, 'Verification of a Rapid Assessment Tool and the Continuation of an Intertidal Monitoring Study in Port Jackson, Sydney', Conference Proceedings and Handbook (2006) [E1]
Co-authors Hugh Dunstan
2006 Greenwood ME, Macfarlane GR, 'Out of the fryingpan into the fire: probable effects of salinity on three dominant coastal macrophytes', Book of Abstracts (2006) [E3]
2006 Macfarlane GR, Koller CE, 'Biomarkers of heavy metal contaminations in the grey mangrove Avicennia marina', Book of Abstracts (2006) [E3]
2006 Macfarlane GR, Koller CE, 'Biological Indicators of Heavy Metal Stress in the Grey Mangrove, Avicennia Marina', Abstracts (2006) [E3]
2006 Russell RA, Linz K-B, Markich S, Gifford SP, O'Connor W, Dunstan RH, Macfarlane GR, 'Microanalysis of Pearl and Shell of the Akoya Pearl Oyster', Abstracts (2006) [E3]
Co-authors Hugh Dunstan
2005 Howe A, Rodriguez JF, Macfarlane GR, 'Vegetation-Sediment-Flow Interactions In Estuarine Wetlands', MODSIM05 : International Congress on Modelling and Simulation : advances and applications for management and decision making, Melbourne, 12-15 December (2005) [E1]
Citations Scopus - 2Web of Science - 1
Co-authors Jose Rodriguez
2005 Macfarlane GR, Markwell KW, Date-Huxtable EM, 'Encouraging students to 'think as biologists': independent field-based projects and peer assessment as a deep learning strategy', Proceedings of the Blended Learning in Science Teaching and Learning Symposium (2005) [E1]
2005 Macfarlane GR, Markwell KW, 'Towards inclusive teaching practice in science education in relation to gender and sexuality', Uniserve Science 2005: Blended Learning in Science Teaching and Learning (2005) [E3]
2005 Macfarlane GR, Rogers LJ, Kaplan G, Blomberg S, 'Same-sex sexual behaviour in birds: expression is related to social mating system', 3rd International behavioural development symposium (2005) [E3]
2005 Linz K-B, Gifford SP, Dunstan RH, O'Connor W, Macfarlane GR, 'The Effects of Intermittent versus Constant Lead (Pb) Exposure on the Pearl Oyster Pinctada imbricata', Australasian Society for Ecotoxicology Annual Conference. Toxicological approaches to predict and assess ecosystem stress (2005) [E3]
Co-authors Hugh Dunstan
2005 Koller C, Patrick JW, Rose RJ, Offler CE, Macfarlane GR, 'Pteris umbrosa as an arsenic hyperaccumulator?', Australasian Society for Ecotoxicology Annual Conference. Toxicological approaches to predict and assess ecosystem stress (2005) [E3]
Co-authors Tina Offler, John Patrick, Ray Rose
2005 Macfarlane GR, Schreider MJ, McLennan BS, 'Biomarkers of Heavy metal stress in an estuarine crab species', Australasian Society for Ecotoxicology Annual Conference. Toxicological approaches to predict and assess ecosystem stress (2005) [E3]
Co-authors Maria Schreider
2005 Flanagan NA, Reddon A, Macfarlane GR, 'Effects of runnelling on non-target saltmarsh species', Australasian Society of Wetland Scientists Annual Meeting: Australasian Saltmarshes (2005) [E3]
2005 Flanagan NA, Redden AM, Macfarlane GR, Williams R, 'Efficacy of a modiefied Breder trap for sampling nekton in shallow estuarine habitats', Book of Abstracts (2005) [E3]
2005 Greenwood ME, Macfarlane GR, 'Effects of salinity and temperature fluctuations on the germination of three macrophytes in a coastal marsh system designated for restoration', Book of Abstracts (2005) [E3]
2005 Macfarlane GR, Schreider MJ, McLennan BS, 'Biomarkers of heavy metals stress in saltmarshes', Book of Abstracts (2005) [E3]
Co-authors Maria Schreider
2005 Flanagan NA, Redden AM, Macfarlane GR, 'Does runnelling affects saltmarsh fauna? Indications for benthic macroinvertebrates, fishes and decapods in Port Stephens, NSW', Book of Abstracts (2005) [E3]
2005 Gifford SP, Macfarlane GR, O'Connor WA, Dunstan RH, 'Evaluation of the Pearl Oyster Pinctada Imbricata as a Remediator of Dissolved Lead and Zinc in Coastal Ecosystems', Proceedings 3rd European Bioremediation Conference (2005) [E2]
Co-authors Hugh Dunstan
2005 Gifford SP, Macfarlane GR, O'Connor WA, Dunstan RH, 'Zooremediation:Using Animals to Remediate Aquatic Environments', Proceedings of 3rd European Bioremediation Conference (2005) [E2]
Co-authors Hugh Dunstan
2005 Greenwood ME, Macfarlane GR, Toia RF, 'Controlled study and field assessment data: do the results match up?', Society of Wetland Scientists 26th Annual Meeting (2005) [E3]
2005 Gifford SP, Dunstan RH, O'Connor W, Macfarlane GR, 'Uptake of the veterinary antibiotic sulphamethazine by the pearl oyster, P. imbricata', Toxicological approaches to predict and assess ecosystem stress (2005) [E3]
Co-authors Hugh Dunstan
2005 McHenry CR, Macfarlane GR, 'Odysseys in Pastryland - a bird's eye view of microevolution', Uniserve Science 2005 Symposium. Blended Learning: Design and implementation (2005) [E3]
2005 O'Connor W, Gifford SP, Macfarlane GR, Dunstan RH, 'Mollusc culture development on the Australian east coast: issues, perceptions and management measures', World Aquaculture 2005: International peace and development through aquaculture (2005) [E3]
Co-authors Hugh Dunstan
2004 Gifford SP, Dunstan RH, O'Connor WA, Macfarlane GR, Gosavi K, Toia RF, 'Monitoring for potential negative environmental impacts associated with pearl aquaculture', Abstract Book, Aquaculture 2004 (2004) [E3]
Co-authors Hugh Dunstan
2004 Gifford SP, Dunstan RH, O'Connor W, Macfarlane GR, Toia RF, 'Pearl aquaculture: the first candidate for 'Zooremediation'?', Abstract Book, Aquaculture 2004 (2004) [E3]
Co-authors Hugh Dunstan
2004 Gifford SP, Dunstan RH, O'Connor W, Macfarlane GR, 'Are environmental monitoring programs necessary for bivalve aquaculture? The Port Stephens, Australia, experience', Abstract Book, Australasian Aquaculture 2004 (2004) [E3]
Co-authors Hugh Dunstan
2004 Greenwood ME, Macfarlane GR, Toia RF, 'Germination characteristics of two Juncus species, with regard to salinity and temperature', Charting the Future: A Quarter Century of Lessons Learned (2004) [E3]
2004 Greenwood M, Macfarlane GR, 'Lost translation: pitfalls associated with using Northern Hemisphere data to answer Australasian questions', Conference Abstracts, Utrecht 2004 Wetlands Conference (2004) [E3]
2004 Gifford SP, Macfarlane GR, O'Connor W, Dunstan RH, 'Effects of the pollutants lead, zinc, hexadecane and octocasane on total and shell growth in the akoya pearl oyster, Pinctada imbricata', Molluscan Megadiversity: Sea, Land and Freshwater (2004) [E3]
Co-authors Hugh Dunstan
2004 Macfarlane GR, 'Group projects in ecology foster a deep learning approach', Proceedings of Scholarly Inquiry into Science Teaching and Learning (2004) [E3]
2003 Gifford SP, Dunstan RH, Macfarlane GR, O'Connor WA, Toia RF, 'Pearl Aquaculture: Profitable nutrient remediation of coastal ecosystems', Solutions to Pollution: Programme Abstract Book (2003) [E3]
Co-authors Hugh Dunstan
2003 Macfarlane GR, Albrecht GA, Shea M, Dapas F, 'Freshwater Mussels as indicators of ecosystem health in the Upper Hunter Region of New South Wales', Proceedings of the Airs Waters Places Transdisciplinary Conference on Ecosystem Health in Australia (2003) [E1]
2002 Macfarlane G, King SA, 'Observer presence influences the behaviour of the Semaphore crab' (2002) [E3]
2001 Macfarlane GR, Burchett MD, 'Assessing effects of petroleum oil on intertidal invertebrate communities in Sydney Harbour: preparedness pays off' (2001) [E3]
2001 Macfarlane GR, 'Potential biological indicators of heavy metal stress in mangrove ecosystems' (2001) [E3]
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Research Supervision

Number of supervisions

Completed5
Current4

Total current UON EFTSL

PhD1.5

Current Supervision

Commenced Level of Study Research Title / Program / Supervisor Type
2016 PhD Assessing of Potential Estrogenic Effects on Freshwater Mussel (Hyridelladepressa) in New South Wales, Australia
PhD (Environmental Sc), Faculty of Science, The University of Newcastle
Principal Supervisor
2014 PhD Molecular cloning and characterisation of insulin-like growth factor binding protein (IGFBP) genes in the Sydney rock oyster
PhD (Environmental Sc), Faculty of Science, The University of Newcastle
Co-Supervisor
2012 PhD Investigation of the Molecular Mechanisms Underlying Chemical-induced Endocrine Disruption in Sydney Rock Oysters (Saccostrea glomerata)
PhD (Environmental Sc), Faculty of Science, The University of Newcastle
Co-Supervisor
2011 PhD Responses of Invasive Birds to Control: The Case of Common Mynas in Australia
PhD (Psychology - Science), Faculty of Science, The University of Newcastle
Co-Supervisor

Past Supervision

Year Level of Study Research Title / Program / Supervisor Type
2016 PhD Influence of Metal Exposure History on Metal Tolerance in the Sydney Rock Oyster (Saccostrea Glomerata)
PhD (Biological Sciences), Faculty of Science, The University of Newcastle
Principal Supervisor
2011 PhD Molluscan Biomonitor for Quantification and Impact Assessment of Estrogenic and Metallic Contaminants in Australian Marine Ecosystems
PhD (Environmental Sc), Faculty of Science, The University of Newcastle
Principal Supervisor
2008 PhD Predicting the Effects of Salinity on Three Dominant Macrophytes: An Anticipatory Approach to the Restoration of Degraded Coastal Wetlands in NSW, Australia
PhD (Environmental Sc), Faculty of Science, The University of Newcastle
Sole Supervisor
2006 PhD Environmental Considerations Associated with the Development of an Akoya Pearl Aquaculture Industry in NSW, Australia
PhD (Biological Sciences), Faculty of Science, The University of Newcastle
Co-Supervisor
2005 PhD Effects of Runnelling on Saltmarsh in Port Stephens, New South Wales, Australia
PhD (Biological Sciences), Faculty of Science, The University of Newcastle
Co-Supervisor
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Dr Geoffrey MacFarlane

Position

Senior Lecturer
School of Environmental and Life Sciences
Faculty of Science

Focus area

Environmental Science and Management

Contact Details

Email geoff.macfarlane@newcastle.edu.au
Phone (02) 4921 7858
Fax (02) 4921 8977

Office

Room LS2-47
Building Life Sciences.
Location Callaghan
University Drive
Callaghan, NSW 2308
Australia
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