Dr Diane Sparkes

Research Associate

School of Environmental and Life Sciences (Biological Sciences)

Career Summary

Biography

Research Expertise
Metabolomics

Fields of Research

Code Description Percentage
060199 Biochemistry and Cell Biology not elsewhere classified 50
110199 Medical Biochemistry and Metabolomics not elsewhere classified 50

Professional Experience

UON Appointment

Title Organisation / Department
Research Associate University of Newcastle
School of Environmental and Life Sciences
Australia
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Publications

For publications that are currently unpublished or in-press, details are shown in italics.


Chapter (2 outputs)

Year Citation Altmetrics Link
2006 Sparkes DL, Robinson GL, Roberts TK, Dunstan RH, 'General Health and Associated Biochemistry in a Visual-Perceptual Subtype of Dyslexia', Learning Disabilities: New Rsearch, Nova Science Publishers, Hauppauge 81-98 (2006) [B1]
Co-authors Tim Roberts, Hugh Dunstan
2004 Robinson GL, Foreman PJ, Dear KB, Sparkes DL, 'The family incidence of Visual-Perceptual subtype of Dyslexia', Focus on Dyslexia Research, Nova Publishers, New York 27-40 (2004) [B1]
Co-authors Phil Foreman

Journal article (10 outputs)

Year Citation Altmetrics Link
2016 Dunstan RH, Sparkes DL, Dascombe BJ, Macdonald MM, Evans CA, Stevens CJ, et al., 'Sweat facilitated amino acid losses in male athletes during exercise at 32-34°C', PLoS ONE, 11 (2016)

© 2016 Dunstan et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and re... [more]

© 2016 Dunstan et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.Sweat contains amino acids and electrolytes derived from plasma and athletes can lose 1-2L of sweat per hour during exercise. Sweat may also contain contributions of amino acids as well as urea, sodium and potassium from the natural moisturizing factors (NMF) produced in the stratum corneum. In preliminary experiments, one participant was tested on three separate occasions to compare sweat composition with surface water washings from the same area of skin to assess contributions from NMF. Two participants performed a 40 minute self-paced cycle session with sweat collected from cleansed skin at regular intervals to assess the contributions to the sweat load from NMF over the period of exercise. The main study investigated sweat amino acid composition collected from nineteen male athletes following standardised endurance exercise regimes at 32-34°C and 20-30% RH. Plasma was also collected from ten of the athletes to compare sweat and plasma composition of amino acids. The amino acid profiles of the skin washings were similar to the sweat, suggesting that the NMF could contribute certain amino acids into sweat. Since the sweat collected from athletes contained some amino acid contributions from the skin, this fluid was subsequently referred to as "faux" sweat. Samples taken over 40 minutes of exercise showed that these contributions diminished over time and were minimal at 35 minutes. In the main study, the faux sweat samples collected from the athletes with minimal NMF contributions, were characterised by relatively high levels of serine, histidine, ornithine, glycine and alanine compared with the corresponding levels measured in the plasma. Aspartic acid was detected in faux sweat but not in the plasma. Glutamine and proline were lower in the faux sweat than plasma in all the athletes. Three phenotypic groups of athletes were defined based on faux sweat volumes and composition profiles of amino acids with varying relative abundances of histidine, serine, glycine and ornithine. It was concluded that for some individuals, faux sweat resulting from exercise at 32-34°C and 20-30% RH posed a potentially significant source of amino acid loss.

DOI 10.1371/journal.pone.0167844
Co-authors Craig Evans, Tim Roberts, Hugh Dunstan
2015 Dunstan RH, Sparkes DL, Dascombe BJ, Evans CA, Macdonald MM, Crompton M, et al., 'Sweat facilitated losses of amino acids in Standardbred horses and the application of supplementation strategies to maintain condition during training', Comparative Exercise Physiology, 11 201-212 (2015) [C1]
DOI 10.3920/CEP150027
Co-authors Tim Roberts, Hugh Dunstan, Ben Dascombe, Craig Evans
2014 Dunstan RH, Sparkes DL, Roberts TK, Dascombe BJ, 'Preliminary Evaluations of a Complex Amino Acid Supplement, Fatigue Reviva, to Reduce Fatigue in a Group of Professional Male Athletes and a Group of Males Recruited from the General Public', Food and Nutrition Sciences, 5 231-235 (2014) [C1]
DOI 10.4236/fns.2014.52028
Co-authors Hugh Dunstan, Ben Dascombe, Tim Roberts
2013 Dunstan RH, Sparkes DL, Roberts TK, Crompton MJ, Gottfries J, Dascombe BJ, 'Development of a complex amino acid supplement, Fatigue Reviva (TM), for oral ingestion: initial evaluations of product concept and impact on symptoms of sub-health in a group of males', NUTRITION JOURNAL, 12 (2013) [C1]
DOI 10.1186/1475-2891-12-115
Citations Scopus - 4Web of Science - 4
Co-authors Hugh Dunstan, Tim Roberts, Ben Dascombe
2012 Dunstan RH, Sparkes DL, Wratten C, Denham JW, Gottfries J, Roberts TK, MacDonald MM, 'Metabolic, health and lifestyle profiling of breast cancer radiotherapy patients and the risk of developing fatigue', Journal of Cancer Therapy, 3 731-740 (2012) [C1]
Co-authors Hugh Dunstan, Tim Roberts
2011 Dunstan RH, Sparkes DL, MacDonald MM, Roberts TK, Wratten C, Kumar M, et al., 'Altered amino acid homeostasis and the development of fatigue by breast cancer radiotherapy patients: A pilot study', Clinical Biochemistry, 44 208-215 (2011) [C1]
DOI 10.1016/j.clinbiochem.2010.10.002
Citations Scopus - 4Web of Science - 4
Co-authors Surinder Baines, Tony Rothkirch, Hugh Dunstan, Jim Denham, Tim Roberts
2008 Evans CA, Dunstan RH, Rothkirch TB, Roberts TK, Reichelt KL, Cosford RE, et al., 'Altered amino acid excretion in children with autism', Nutritional Neuroscience, 11 9-17 (2008) [C1]
DOI 10.1179/147683008x301360
Citations Scopus - 20Web of Science - 19
Co-authors Tim Roberts, Craig Evans, Tony Rothkirch, Hugh Dunstan
2003 Sparkes DL, Robinson GL, Dunstan RH, Roberts TK, 'Plasma Cholesterol Levels And Irlen Syndrome: Preliminary Study of 10- to 17-yr-old Students', Perceptual And Motor Skills, Vol 97 743-752 (2003) [C1]
DOI 10.2466/PMS.97.7.743-752
Citations Scopus - 2Web of Science - 1
Co-authors Hugh Dunstan, Tim Roberts
2003 Sparkes DL, 'Biochemical anomalies in people with visual processing problems causing reading difficulties', Australian Journal of Psychology, 55 108-108 (2003)
2001 Robinson GL, Conway RN, Sparkes DL, 'Visual dysfunction in people with dyslexia: Essential fatty acid metabolism, possible immune system system dysfunction and the role of diet', Journal of the South Pacific Educators in Vision Impairment, 2 (1) 8-16 (2001) [C3]
Show 7 more journal articles

Conference (2 outputs)

Year Citation Altmetrics Link
2004 Sparkes DL, Robinson GL, Dunstan RH, Roberts TK, 'Biochemical anomalies in people with a visual sub-type of dyslexia', CDROM (2004) [E1]
Co-authors Hugh Dunstan, Tim Roberts
2004 Robinson GL, Sparkes DL, Roberts TK, Dunstan RH, 'Biochemical anomalies in people with Irlen Syndrome', Conference Paper (2004) [E2]
Co-authors Tim Roberts, Hugh Dunstan
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Dr Diane Sparkes

Position

Research Associate
School of Environmental and Life Sciences
Faculty of Science

Focus area

Biological Sciences

Contact Details

Email diane.sparkes@newcastle.edu.au
Phone (02) 4913 8081

Office

Room LS402
Building Life Sciences Building.
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